KR20160083742A - Emt 저해제 스크리닝 방법 - Google Patents

Emt 저해제 스크리닝 방법 Download PDF

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KR20160083742A
KR20160083742A KR1020150000243A KR20150000243A KR20160083742A KR 20160083742 A KR20160083742 A KR 20160083742A KR 1020150000243 A KR1020150000243 A KR 1020150000243A KR 20150000243 A KR20150000243 A KR 20150000243A KR 20160083742 A KR20160083742 A KR 20160083742A
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송지애
김성훈
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Abstract

본 발명은 EPRS의 신규한 용도로서 이를 이용한 EMT 저해제 스크리닝 방법에 관한 것으로, 더욱 상세하게는 (a) EPRS(Glutamyl-prolyl-tRNA synthetase) 전체 또는 이의 단편, snail 1 단백질 및 시험제제를 접촉시키는 단계; 및 (b) 상기 EPRS와 snail 1의 결합수준의 변화를 측정하는 단계를 포함하는 EMT(epithelial-mesenchymal transition) 저해제 스크리닝 방법에 관한 것이다. 본 발명은의 방법은 암을 포함하여 다양한 EMT 관련 질환의 새로운 치료제의 개발에 유용하게 이용될 수 있어 산업상 이용가능성이 크다.

Description

EMT 저해제 스크리닝 방법{Method for screening EMT inhibitor}
본 발명은 EMT 저해제 스크리닝 방법에 관한 것으로, 더욱 상세하게는 (a) EPRS(Glutamyl-prolyl-tRNA synthetase) 전체 또는 이의 단편, snail 1 단백질 및 시험제제를 접촉시키는 단계; 및 (b) 상기 EPRS와 snail 1의 결합수준의 변화를 측정하는 단계를 포함하는 EMT(epithelial-mesenchymal transition) 저해제 스크리닝 방법에 관한 것이다.
상피-중간엽 전환(epithelial-mesenchymal transition; EMT)은 정상적인 배아 발달 과정에서 발생하는 현상으로, 세포가 상피성(epithelial) 세포 표현형을 상실하고 이동성이 높은 중간엽성(mesenchymal) 세포 표현형으로 전환하는 과정을 말한다. 하지만 이것이 비가역적으로 진행될 경우에는 심장, 간, 신장 및 혈관 기능 부전을 초래할 뿐 아니라 악성 종양의 전환에도 관여하는 것으로 알려져 있다. EMT가 일어나면 상피세포가 세포의 위쪽과 기저쪽이 구분되는 극성을 잃고 세포 모양이 정방형에서 섬유아세포형으로 바뀌고 상피 세포 마커는 감소하고 간엽 세포 마커가 증가한다. 최근에 EMT가 태생기의 조직 형성과 분화 외에도 조직의 재생과 섬유화, 암의 발생과 전환 과정에 다양한 역할을 한다는 것이 알려져 있다.
스네일(Snail)은 현재까지 암의 전이과정에서 침입의 억제자로서 알려진 이-카데린(E-cadherin)을 억제함으로써 EMT 과정에 관여함이 알려져 있으며 또한 태아기의 중배엽과 신경관 형성에 있어서도 관여함이 알려져 있다. 지금까지 알려진 실험결과로부터 스네일은 흑색종, 방광암, 직장암, 췌장암 등에 관여함이 증명되었다 [Cano, A. et al. Nat Cell Biol 2, 76-83 (2000); Batlle, E. et al. Nat Cell Biol 2, 84-9 (2000); Poser, I. et al. J Biol Chem 276, 24661-6 (2001); De Craene, B. et al. Cancer Res 65, 6237-44 (2005)]. 이러한 종양들에서는 이-카데린의 발현양이 현저히 줄어들었음을 알 수 있는데 이 배경에는 스네일 단백질의 대량발현이 관련되었음이 알려졌다. 이러한 스네일 유전자의 발현은 전사레벨에서 티지에프(TGF)와 웬트(Wnt) 같은 신호전달계에서 조절된다.
TGF-베타(TGF-β)는 상처 치유에 있어서 주요한 성장 인자 중 하나이다 (O'Kane (1997) Int J Biochem Cell Biol 29:79-89 에 요약). 과립화 단계 동안에, TGF-베타가 상처 부위에서 혈소판으로부터 나온다. 이때 TGF-베타는 대식세포에서 그 자신의 생성을 조절하고, 기타 성장 인자의 분비를 예를 들어 단핵구에 의해 유도한다. 상처 치유 동안 가장 중요한 기능에는, 염증 세포의 화학주성 촉진, 세포외 기질의 합성, 및 상처 치유 프로세스에 관여하는 모든 중요한 세포 유형의 유전자 발현, 증식 및 분화 조절이 포함된다. 병리 상태 하에서, 이러한 TGF-베타-매개된 효과, 특히 세포외 기질 (ECM) 의 생성 조절로, 섬유증 또는 피부내 상처를 야기할 수 있다 (Border (1994) N Engl J Med 331:1286-1292).
섬유증 질병, 당뇨병성 신병증 및 사구체신염에 있어서, TGF-베타가 신장세포 비대 및 세포외 기질의 병원성 축적을 촉진하는 것이 보고되어져 있다. 항-TGF-베타 항체를 이용한 치료로 TGF-베타 신호경로의 중단은, 혈관사이 기질의 확장, 신장 기능의 점진적인 감소를 방지하고, 당뇨병이 있는 동물에게서 당뇨 사구체병의 확립된 병변을 감소시킨다 (Border (1990) 346: 371-374, Yu (2004) Kind-ney Int 66: 1774-1784, Fukasawah (2004) Kindney Int 65: 63-74, Sharma (1996) Diabetes 45: 522-530). TGF-베타는 또한 간 섬유증에서 중요한 역할을 한다. 간 섬유증 발생에 있어서 필수적인, 간경화 발생 과정에서 세포외 기질의 주요 생성자인, 간 별형 세포의 근섬유모세포 생성을 위한 활성화가 TGF-베타에 의해 고무된다. TGF-베타 신호경로의 중단은 실험 모델에서 섬유증을 감소시킨다는 것이 마찬가지로 여기에서 나타나져 있다 (Yata (2002) Hepatology 35:1022-1030; Arias (2003) BMC Gastroenterol 3:29).
TGF-베타는 또한 암 형성에서 주요 기능을 맡는다 (Derynck (2001) Nature Genetics: 29: 117-129; Elliott (2005) J Clin Onc 23: 2078-2093 에 요약됨). 암 발생 초기 단계에서, TGF 베타는 암 형성에 대응한다. 이 종양-억제 활성은 주로 TGF-베타의 상피세포 분할 억제능에 토대를 둔다. 그에 반해, TGF-베타는 암 성장 및 후기 종양 단계에서 전이 형성을 촉진한다. 이는 대부분의 상피 종양이 TGF-베타의 성장-억제 작용에 대한 저항성을 발전시키고, TGF-베타가 동시에 다른 메카니즘을 통해 암 세포의 성장을 지지한다는 점에 기인할 수 있다. 이러한 메카니즘에는 혈관형성 촉진, 면역-억제 작용 (종양 세포가 면역계의 제어 기능을 모면하도록 지지함 (면역감시)), 및 전이의 형성 및 침투 조장이 포함된다. 종양 세포의 침투 표현형 형성은 전이 형성에 대한 주된 전제조건이다. TGF-베타는 세포 부착, 운동성 및 세포외 기질의 형성을 조절하는 이의 능력을 통해 상기 프로세스를 촉진시킨다. 더욱이, TGF-베타는 세포 상피 표현형에서 침투성 중간엽 표현형으로의 변이를 유도한다 (상피 중간엽 변이　=　EMT). 암 성장의 촉진에서 TGF-베타가 수행하는 중요한 역할은 또한, 강한 TGF-베타 발현과 열악한 예후 사이의 연관성을 보여주는 조사로 증명된다. 증가된 TGF-베타 수준은 그중에서도 특히 전립샘, 유방, 창자 및 폐암을 앓는 환자에게서 발견되어 왔다 (Wikstroem (1998) Prostate 37: 19-29; Hasegawa (2001) Cancer 91: 964-971; Friedman (1995), Cancer Epidemiol Biomarkers Prev. 4:549-54).
아미노아실 tRNA 합성효소(aminoacyl-tRNA synthetase)는 특정 아미노산을 대응되는 tRNA에 정확하게 결합시키는 효소로서, 단백질 합성계에서는 꼭 필요하다. 아미노산과 tRNA을 결합시키는 과정은 크게 두 단계의 과정으로 나뉘는데, 첫 번째 과정은 하나의 ATP를 소모하여, 아미노산을 아미노아실아데닐레이트로 활성화하는 단계이고, 두 번째 과정은 활성화된 아미노산을 tRNA에 전달하는 단계이다. 포유동물의 아미노아실 tRNA 합성효소는 원핵생물의 아미노아실 tRNA 합성효소와 역할은 비슷하지만, 다른 부가적인 도메인을 가진다. 이 부가적인 도메인은 아미노아실 티알엔에이 합성효소나 다른 조절인자들과의 다양한 복합체 형성에 관여한다. 이런 구조적인 복잡성이 아미노아실 tRNA 합성효소의 기능적인 다양함과 사람의 여러 가지 질병과 관련이 있다는 것이 최근 밝혀졌다.
본 발명자들은 암, 섬유증 등의 다양한 EMT 관련 질환의 효과적인 치료 방법 및 치료물질에 대해 연구하던 중, EPRS와 Snail 1의 물리적 상호작용으로 인해 EMT(epithelial- mesenchymal transition)가 활성화된다는 EPRS의 신규한 기능을 발견하고 EPRS와 Snail 1의 결합을 저해하면 EMT가 억제됨을 확인하여 본 발명을 완성하였다.
따라서 본 발명의 목적은 (a) EPRS(Glutamyl-prolyl-tRNA synthetase) 전체 또는 서열번호 2 내지 서열번호 6으로 이루어지는 군에서 선택된 어느 하나의 아미노산 서열을 가지는 이의 단편, snail 1 단백질 및 시험제제를 접촉시키는 단계; 및 (b) 상기 EPRS와 snail 1의 결합수준의 변화를 측정하는 단계를 포함하는 EMT(epithelial-mesenchymal transition) 저해제 스크리닝 방법을 제공하는 것이다.
상기의 목적을 달성하기 위하여, 본 발명은 (a) EPRS(Glutamyl-prolyl-tRNA synthetase) 전체 또는 서열번호 2 내지 서열번호 6 으로 이루어지는 군에서 선택된 어느 하나의 아미노산 서열을 가지는 이의 단편, snail 1 단백질 및 시험제제를 접촉시키는 단계; 및 (b) 상기 EPRS와 snail 1의 결합수준의 변화를 측정하는 단계를 포함하는 EMT(epithelial-mesenchymal transition) 저해제 스크리닝 방법을 제공한다.
이하 본 발명을 상세히 설명한다.
본 발명자들은 EPRS가 snail 1과 상호작용하여 EMT경로를 조절한다는 사실을 처음으로 규명하였으며, 이는 본 명세서에서 최초로 공개하는 것이다.
정의
다른 정의가 없는 한, 본 명세서에 사용된 모든 기술적 및 과학적 용어는 당업자들에 의해 통상적으로 이해되는 동일한 의미를 가진다. 다음의 참고문헌은 본 발명의 명세서에 사용된 여러 용어들의 일반적인 정의를 갖는 기술(skill)의 하나를 제공한다. Singleton et al., DICTIONARY OF MICROBIOLOGY AND MOLECULAR BIOLOGY(2d ed. 1994); THE CAMBRIDGE DICTIONARY OF SCIENCE AND TECHNOLOGY(Walker ed., 1988); 및 Hale ＆ Marham, THE HARPER COLLINS DICTIONARY OF BIOLOGY. 또한 다음의 정의는 본 발명의 실시를 위해 독자(reader)에게 도움을 주기 위해 제공된다.
본 명세서에서 용어 "단백질"은 "폴리펩티드(polypeptide)" 또는 “펩티드(peptide)"와 호환성 있게 사용되며, 예컨대, 자연상태의 단백질에서 일반적으로 발견되는 바와 같이 아미노산 잔기의 중합체를 말한다.
본 명세서에서 용어 "발현(expression)"이라 함은 세포에서 단백질 또는 핵산의 생성을 의미한다.
본 명세서에서 용어 “EPRS(Glutamyl-prolyl-tRNA synthetase)”는 글루타메이트 및 프롤린의 tRNA와의 결합을 촉진하는 아미노아실 tRNA 합성효소의 일종을 의미한다. 아미노아실 tRNA 합성효소 중, 사람의 글루타메이트와 프롤린의 tRNA와의 결합을 촉진하는 효소(EPRS)는 특이하게도 하나의 폴리펩타이드 사슬에 존재한다. 글루타메이트를 촉매하는 도메인은 N 말단 부분에, 프롤린을 촉매하는 도메인은 C 말단에 각각 위치하며, 상기 두 도메인은 WHEP 도메인으로 연결되어 있다. 글루타밀 tRNA 합성효소는 class 1 아미노아실 tRNA 합성효소에 속하고, 프롤린 tRNA 합성효소는 class 2 아미노아실 tRNA 합성효소에 속한다. 두 도메인을 연결하는 WHEP 도메인은 57개의 아미노산 사슬이 3번 반복되어 있는 구조로 구성되어 있는데, 단백질-단백질 또는 단백질-RNA 상호작용을 매개한다. 본 발명의 상기 EPRS 전체 단백질(또는 폴리펩타이드)은 공지의 포유류 유래 EPRS 서열이라면 그 종류가 특별히 제한되지 않으며, 예를 들어 서열번호 1로 표시되는 인간 EPRS 단백질 일 수 있고, 또한 본 발명의 EPRS는 이의 기능적 동등물을 포함한다.
본 명세서에서 용어‘EPRS 단편’은 상기 EPRS 전체 폴리펩티드로부터의 단편 서열을 의미하는 것으로서, 바람직하게 글루타메이트 결합 도메인(ERS domain) 또는/및 프롤린 결합 도메인(PRS domain)을 포함하는 서열이라면 그 서열이 특별히 제한되지 않으나, 예를 들어 서열번호 2(ERS-WHEP domain, 도 14의 “EPRS(△PRS)”즉, 서열번호 1로 표시되는 EPRS 서열에서 1 ~ 1024번째 아미노산 서열 중 발췌), 서열번호 3(ERS domain, 도 14의 “EPRS(△WHEP, PRS)”즉, 서열번호 1로 표시되는 EPRS 서열에서 1 ~ 682번째 아미노산 서열을 포함하여 발췌), 서열번호 4(WHEP-PRS domain, 도 14의 “EPRS(△ERS)”즉, 서열번호 1로 표시되는 EPRS 서열에서 682 ~ 1512번째 아미노산 서열 중 발췌), 서열번호 5(PRS domain, 도 14의 “EPRS(△ERS,WHEP)”즉, 서열번호 1로 표시되는 EPRS 서열에서 1024 ~ 1512번째 아미노산 서열을 포함하여 발췌), 서열번호 6(ERS-PRS domain, 도 14의 “EPRS(△WHEP)”, 상기 서열번호 3 및 5의 연결체) 등을 포함한다(Ref; Mol Cell 35, 164-180, 2009). 본 발명에서 상기 EPRS 단편은 가장 바람직하게 프롤린 결합도메인을 포함하는 것일 수 있으며, 예를 들어 서열번호 4, 서열번호 5 및 서열번호 6으로 이루어진 군에서 선택된 어느 하나의 폴리펩티드 일 수 있으나, 이에 제한되지 않는다. 또한 본 발명의 상기 EPRS 단편은 이의 기능적 동등물을 포함한다.
상기 기능적 동등물이란 EPRS 또는 이의 단편의 아미노산 서열과 적어도 70％ 이상, 바람직하게는 80％ 이상, 보다 바람직하게는 90％ 이상의 서열 상동성(즉, 동일성)을 갖는 폴리펩티드를 말한다. 예를 들면, 70％, 71％, 72％, 73％, 74％, 75％, 76％, 77％, 78％, 79％, 80％, 81％, 82％, 83％, 84％, 85％, 86％, 87％, 88％, 89％, 90％, 91％, 92％, 93％, 94％, 95％, 96％, 97％, 98％, 99％, 100％의 서열 상동성을 갖는 폴리펩티드를 포함하는 것으로, 서열번호 1 내지 서열번호 6 으로 이루어진 군에서 선택된 어느 하나의 서열로 표시되는 폴리펩티드와 실질적으로 동질의 생리활성을 나타내는 폴리펩티드를 말한다. 여기서,"실질적으로 동질의 생리활성"이란 Snail, 특히 snail 1과 결합하여 EMT 경로의 진행을 촉발 및 촉진시키는 것을 말한다. 상기 기능적 동등물은 EPRS 또는 이의 단편의 아미노산 서열 중 일부가 부가, 치환 또는 결실의 결과 생성될 것일 수 있다. 상기에서 아미노산의 치환은 바람직하게는 보존적 치환이다. 천연에 존재하는 아미노산의 보존적 치환의 예는 다음과 같다; 지방족 아미노산(Gly, Ala, Pro), 소수성 아미노산(Ile, Leu, Val), 방향족 아미노산(Phe, Tyr, Trp), 산성 아미노산(Asp, Glu), 염기성 아미노산(His, Lys, Arg, Gln, Asn) 및 황함유 아미노산(Cys, Met). 또한 상기 기능적 동등물에는 본 발명의 EPRS 폴리펩티드의 아미노산 서열상에서 아미노산의 일부가 결실된 변형체도 포함된다. 상기 아미노산의 결실 또는 치환은 바람직하게는 EPRS 폴리펩티드의 생리활성에 직접 관여하지 않는 부분에 위치한다. 아울러 상기 EPRS 폴리펩티드의 아미노산 서열의 양 말단 또는 서열 내에 몇몇의 아미노산이 부가된 변형체도 포함된다. 또한 본 발명의 기능적 동등물의 범위에는 본 발명에 따른 폴리펩티드의 기본 골격 및 이의 생리활성을 유지하면서 폴리펩티드의 일부 화학 구조가 변형된 폴리펩티드 유도체도 포함된다. 예를 들어, 본 발명의 폴리펩티드의 안정성, 저장성, 휘발성 또는 용해도 등을 변경시키기 위한 구조변경이 이에 포함된다.
본 명세서에서 서열 상동성 및 동질성은 EPRS의 아미노산 서열(서열번호 1) 또는 이의 단편(서열번호 2 내지 서열번호 6)의 아미노산 서열과 후보 서열을 정렬하고 갭(gaps)을 도입한 후 EPRS의 아미노산 서열에 대한 후보 서열의 아미노산 잔기의 백분율로서 정의된다. 필요한 경우, 최대 백분율 서열 동질성을 수득하기 위하여 서열 동질성의 부분으로서 보존적 치환은 고려하지 않는다. 또한, EPRS 또는 이의 단편의 아미노산 서열의 N-말단, C-말단 또는 내부 신장, 결손 또는 삽입은 서열 동질성 또는 상동성에 영향을 주는 서열로서 해석되지 않는다. 또한, 상기 서열 동질성은 두 개의 폴리펩티드의 아미노산 서열의 유사한 부분을 비교하기 위해 사용되는 일반적인 표준 방법에 의해 결정할 수 있다. BLAST와 같은 컴퓨터 프로그램은 두 개의 폴리펩티드를 각각의 아미노산이 최적으로 매칭되도록 정렬한다(하나 또는 두 서열의 전장서열을 따라 또는 하나 또는 두 서열의 예측된 부분을 따라). 상기 프로그램은 디펄트 오프닝 페널티(default opening penalty) 및 디펄트 갭 페널티(default gap penalty)를 제공하며 컴퓨터 프로그램과 함께 연계되어 사용될 수 있는 PAM250(표준 스코링 매트릭스; Dayhoff et al., in Atlas of Protein Sequence and Structure, vol 5, supp 3, 1978)와 같은 스코링 매트릭스를 제공한다. 예를 들어, 백분율 동질성은 다음과 같이 계산할 수 있다. 일치하는 서열(identical matches)의 총 수에 100을 곱한 다음 대응되는 스팬(matched span) 내의 보다 긴 서열의 길이와 두 서열을 정렬하기 위해 보다 긴 서열 내로 도입된 갭(gaps)의 수의 합으로 나눈다.
바람직하게 본원 발명에서 상기 EPRS의 기능적 동등물은 서열번호 1의 아미노산 서열과 99% 상동서열인 NCBI Gene Bank Accession Number EAW93309.1(서열번호 25), CAI45949.1(서열번호 26), XP_001172425.1(서열번호 27), XP_003807230.1(서열번호 28), XP_009439782.1(서열번호 29), XP_003265132.1(서열번호 30), EAW93310.1(서열번호 31), CAA38224.1(서열번호 32) ; 98% 상동서열인 NCBI Gene Bank Accession Number XP_010366377.1(서열번호 33), XP_005540939.1(서열번호 34), XP_007986597.1(서열번호 35), XP_005540938.1(서열번호 36), XP_007986596.1(서열번호 37), XP_007986598.1(서열번호 38); 97% 상동서열인 NCBI Gene Bank Accession Number XP_003930387.1(서열번호 39), XP_010339237.1(서열번호 40), EHH50530.1(서열번호 41); 96% 상동서열인 NCBI Gene Bank Accession Number XP_002808297.1(서열번호 42), XP_009185375.1(서열번호 43), EHH15538.1(서열번호 44) ; 95% 상동서열인 NCBI Gene Bank Accession Number XP_004028474.1(서열번호 45), XP_008983438.1(서열번호 46); 94% 상동서열인 NCBI Gene Bank Accession Number XP_008053500.1(서열번호 47); 93% 상동서열인 NCBI Gene Bank Accession Number XP_001488980.1(서열번호 48), XP_008541056.1(서열번호 49), XP_849468.1(서열번호 50), XP_004013652.1(서열번호 51), XP_005640882.1(서열번호 52), XP_005981363.1(서열번호 53), XP_005690536.1(서열번호 54), XP_002920053.1(서열번호 55), XP_005981362.1(서열번호 56), XP_004271072.1(서열번호 57), XP_008053499.1(서열번호 58), XP_004415195.1(서열번호 59), XP_004324147.1(서열번호 60), EFB20321.1(서열번호 61), XP_004751515.1(서열번호 62), XP_008683957.1(서열번호 63), XP_003999559.1(서열번호 64), XP_007072912.1(서열번호 65), XP_008586040.1(서열번호 66); 92% 상동서열인 NCBI Gene Bank Accession Number XP_006188215.1(서열번호 67), XP_006198933.1(서열번호 68), XP_004480290.1(서열번호 69), XP_008266633.1(서열번호 70), XP_006056008.1(서열번호 71), XP_007935118.1(서열번호72), XP_007455449.1(서열번호 73), NP_001230249.1(서열번호 74), XP_006056007.1(서열번호 75), XP_005905625.1(서열번호 76), XP_007129327.1(서열번호 77), XP_007172136.1(서열번호 78), XP_005335922.1(서열번호 79), XP_007172135.1(서열번호 80); 91% 상동서열인 NCBI Gene Bank Accession Number XP_003419927.1(서열번호 81), XP_006869760.1(서열번호 82), XP_007521381.1(서열번호 83), XP_005335921.1(서열번호 84), XP_008148043.1(서열번호 85), XP_004439743.1(서열번호 86), XP_004685497.1(서열번호 87), XP_004578688.1(서열번호 88), XP_005879946.1(서열번호 89); 90% 상동서열인 NCBI Gene Bank Accession Number XP_008825021.1(서열번호 90), XP_008825020.1(서열번호 91), XP_004699962.1(서열번호 92), XP_006140298.1(서열번호 93), XP_007642504.1(서열번호 94), ERE73005.1(서열번호 95), AAH94679.1(서열번호 96), EDL13067.1(서열번호 97), XP_008825022.1(서열번호 98); 89% 상동서열인 NCBI Gene Bank Accession Number XP_005348894.1(서열번호 99), AAI41050.1(서열번호 100), NP_084011.1(서열번호 101), XP_006497180.1(서열번호 102), XP_005082493.1(서열번호 103), ERE73006.1(서열번호 104), XP_007636777.1(서열번호 105); 88% 상동서열인 NCBI Gene Bank Accession Number XP_004670165.1(서열번호 106), XP_006916292.1(서열번호 107), XP_006250488.1(서열번호 108), XP_006250487.1(서열번호 109), XP_006094389.1(서열번호 110); 87% 상동서열인 NCBI Gene Bank Accession Number XP_005409334.1(서열번호 111), XP_004626936.1(서열번호 112), XP_004613070.1(서열번호 113), KFO25396.1(서열번호 114); 86% 상동서열인 NCBI Gene Bank Accession Number XP_003474570.1(서열번호 115), XP_005005650.1(서열번호 116), XP_004867452.1(서열번호 117), XP_004878984.1(서열번호 118); 85% 상동서열인 NCBI Gene Bank Accession Number EPQ16188.1(서열번호 119); 84% 상동서열인 NCBI Gene Bank Accession Number NP_001019409.1(서열번호 120); 83% 상동서열인 NCBI Gene Bank Accession Number XP_008177290.1(서열번호 121), XP_009094640.1(서열번호 122), XP_006022406.1(서열번호 123) 등을 포함하며, 이에 제한되지 않는다.
본 발명에 따른 단백질(폴리펩티드)는 천연에서 추출하거나 유전공학적 방법에 의해 작제될 수 있다. 예를 들면, 통상적인 방법에 따라 상기 폴리펩티드 또는 이의 기능적 동등물을 암호화하는 핵산(예를 들어, EPRS의 경우 서열번호 8, EPRS 단편의 경우 서열번호 9 내지 13 으로 이루어진 군에서 선택된 어느 하나)을 작제한다. 상기 핵산은 적절한 프라이머를 사용하여 PCR 증폭함으로써 작제 할 수 있다. 다른 방법으로 당업계에 공지된 표준 방법에 의해, 예컨대, 자동 DNA 합성기(Biosearch 또는 Applied Biosystems 사에서 판매하는 것)을 사용하여 DNA 서열을 합성할 수도 있다. 작제된 핵산은 이에 작동가능하게 연결되어 (operatively linked) 핵산의 발현을 조절하는 하나 이상의 발현 조절 서열(expression control sequence)(예: 프로모터, 인핸서 등)을 포함하는 벡터에 삽입시키고, 이로부터 형성된 재조합 발현 벡터로 숙주세포를 형질전환시킨다. 생성된 형질전환체를 상기 핵산이 발현되기에 적절한 배지 및 조건 하에서 배양하여, 배양물로부터 상기 핵산에 의해 발현된, 실질적으로 순수한 폴리펩티드를 회수한다. 상기 회수는 당업계에 공지된 방법(예컨대, 크로마토그래피)을 이용하여 수행할 수 있다. 상기에서 "실질적으로 순수한 폴리펩티드(substally pure polypeptide)"라 함은 본 발명에 따른 폴리펩티드가 숙주세포로부터 유래된 어떠한 다른 단백질도 실질적으로 포함하지 않는 것을 의미한다. 본 발명의 폴리펩티드 합성을 위한 유전공학적 방법은 다음의 문헌을 참고할 수 있다: Maniatis et al., Molecular Cloning; A laboratory Manual, Cold Spring Harbor laboratory, 1982; Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, N.Y., Second(1998) and Third(2000) Editions; Gene Expression Technology, Method in Enzymology, Genetics and Molecular Biology, Method in Enzymology, Guthrie ＆ Fink(eds.), Academic Press, San Diego, Calif, 1991; 및 Hitzeman et al., J. Biol. Chem., 255:12073-12080, 1990.
또한, 본 발명의 폴리펩티드는 당업계에 공지된 화학적 합성(Creighton, Proteins; Structures and Molecular Principles, W. H. Freeman and Co., NY, 1983)에 의해 쉽게 제조될 수 있다. 대표적인 방법으로서 이들로 한정되는 것은 아니지만 액체 또는 고체상 합성, 단편 응축, F-MOC 또는 T-BOC 화학법이 포함된다(Chemical Approaches to the Synthesis of Peptides and Proteins, Williams et al., Eds., CRC Press, Boca Raton Florida, 1997; A Practical Approach, Athert on ＆ Sheppard, Eds., IRL Press, Oxford, England, 1989).
본 발명에서 '프로모터'란 특정한 숙주 세포에서 작동 가능하게 연결된 핵산 서열의 발현을 조절하는 DNA 서열을 의미하며, '작동 가능하게 연결된다(operably linked)'는 것은 하나의 핵산 단편이 다른 핵산 단편과 결합되어 그의 기능 또는 발현이 다른 핵산 단편에 의해 영향을 받는 것을 말한다. 아울러, 전사를 조절하기 위한 임의의 오퍼레이터 서열, 적합한 mRNA 리보좀 결합 부위를 코딩하는 서열 및 전사 및 해독의 종결을 조절하는 서열을 추가로 포함할 수 있다. 상기 프로모터로는 모든 시간대에 상시적으로 목적 유전자의 발현을 유도하는 프로모터(constitutive promoter) 또는 특정한 위치, 시기에 목적 유전자의 발현을 유도하는 프로모터(inducible promoter)를 사용할 수 있다.
본 발명에서 "숙주세포(host cell)"이라 함은 임의의 수단(예: 전기충격법, 칼슘 포스파타제 침전법, 미세주입법, 형질전환법, 바이러스 감염 등)에 의해 세포 내로 도입된 이종성 DNA를 포함하는 원핵 또는 진핵 세포를 말한다.
본 발명에서 "핵산", "DNA 서열" 또는 "폴리뉴클레오티드" 는 단일-또는 이중-가닥의 형태로 된 데옥시리보뉴클레오티드 또는 리보뉴클레오티드를 말한다. 다른 제한이 없는 한, 자연적으로 생성되는 뉴클레오티드와 비슷한 방법으로 핵산에 혼성화되는 자연적 뉴클레오티드의 공지된 아날로그도 포함된다.
본 발명에서 "EPRS를 암호화하는 폴리뉴클레오티드"는 서열번호 1로 표시되는 아미노산 서열 또는 이와 적어도 70％ 이상의 서열 상동성을 갖는 아미노산서열을 암호화하는 염기서열을 가질 수 있다. 상기 핵산은 DNA, cDNA 및 RNA 서열을 모두 포함한다. 즉, 상기 폴리뉴클레오티드는 서열번호 1의 아미노산 서열 또는 이와 적어도 70％ 이상의 상동성을 갖는 아미노산 서열을 암호화하는 염기서열을 갖거나 상기 염기서열에 상보적인 염기서열을 가질 수 있다. 바람직하게는 서열번호 8로 표시되는 염기서열을 갖는 것 일 수 있다. 상기 핵산은 천연에서 분리되거나 위에서 기술한 바와 같이 유전공학적인 방법에 의해 제조될 수 있다.
또한 본 발명에서 EPRS 단편을 암호화하는 폴리뉴클레오티드는 서열번호 2 내지 6 으로 이루어진 군에서 선택된 어느 하나의 아미노산 서열 또는 이와 적어도 70% 이상의 서열 상동성을 갖는 아미노산 서열을 암호화하는 염기서열을 가질 수 있다. 바람직하게 서열번호 9 내지 13 으로 이루어진 군에서 선택된 어느 하나의 염기서열을 갖는 것일 수 있다. 상기 핵산은 천연에서 분리되거나 위에서 기술한 바와 같이 유전공학적인 방법에 의해 제조될 수 있다.
본 발명에서 용어 "상동체(homologues)"는 단백질 및/또는 단백질 서열을 언급하는 경우에는 공통의 조상 단백질(common ancestral protein) 또는 단백질 서열에서 자연적으로 또는 인위적으로 유래된 것을 나타낸다. 유사하게 핵산 및/또는 핵산 서열은 그들이 공통의 조상 핵산 또는 핵산 서열로부터 자연적으로 또는 인위적으로 유래되었을 때 상동하다.
본 명세서에서 용어 "아날로그(analog)"는 표준 물질(reference molecule)과 구조적으로 유사하지만, 표준 물질의 특이적 치환기가 치환에 의해 대체됨으로써 표적이나 조절 방법이 변형된 물질을 말한다. 표준물질과 비교할 때, 아날로그는 당업자에 의해 예상될 수 있는 바와 같이 동일 또는 유사하거나 향상된 유용성(utility)를 가진다. 향상된 성질(예: 표적 물질에 대한 더 높은 결합 친화력)을 갖는 공지의 화합물 변이체를 규명하기 위한 아날로그의 합성 및 스크리닝은 약리 화학적 분야에 공지된 방법이다.
본 명세서에서 용어 "접촉(contacting)"은 이의 일반적인 의미이며, 2개 이상의 제제(예: 2개의 폴리펩티드)를 결합(combine)시키거나, 제제와 세포(예; 단백질과 세포)를 결합시키는 것을 말한다. 접촉은 시험관 내(in vitro)에서 일어날 수 있다. 예컨대, 시험관(test tube) 또는 다른 컨테이너(container)에서 2개 이상의 제제를 결합시키거나 시험 제제와 세포 또는 세포 용해물과 시험 제제를 결합시키는 것이다. 또한 접촉은 세포 또는 인시투(in situ)에서 일어날 수도 있다. 예컨대, 2개의 폴리펩티드를 암호화하는 재조합 폴리뉴클레오티드를 세포 내에서 공동발현(coexpresion)시킴으로써 세포 또는 세포 용해물에서 2개의 폴리펩티드를 접촉시키는 것이다.
본 발명에서 "제제(agent)" 또는 "시험 제제(test agent)"라 함은 임의의 물질(substance), 분자(molecule), 원소(element), 화합물(compound), 실재물(entity) 또는 이들의 조합을 포함한다. 예컨대, 이에 제한되지는 않으나, 단백질, 폴리펩티드, 소 유기 물질(small organic molecule), 다당류(polysaccharide), 폴리뉴클레오티드 등을 포함한다. 또한 자연 산물(natural product), 합성 화합물 또는 화학 화합물 또는 2개 이상의 물질의 조합일 수도 있다. 다르게 지정되지 않는 한, 제제, 물질 및 화합물은 호환성 있게(interchangeably) 사용할 수 있다.
보다 구체적으로 본 발명의 스크리닝 방법으로 스크리닝 될 수 있는 시험 제제는, 폴리펩티드, 베타-턴 미메틱(beta-turn mimetics), 다당류, 인지질, 호르몬, 프로스타글란딘, 스테로이드, 방향족 화합물, 헤테로사이클릭 화합물, 벤조디아제핀(benzodiazepines), 올리고머릭 N-치환 글리신(oligomeric N-substituted glycines), 올리고카르바메이트(oligocarbamates), 당류(saccharides), 지방산, 퓨린, 피리미딘 또는 이들의 유도체, 구조적 아날로그 또는 조합을 포함한다. 어떤 시험 제제는 합성 물질일 수 있으며, 다른 시험 제제는 천연물질일 수 있다. 상기 시험 제제는 합성 또는 자연 화합물의 라이브러리를 포함하는 광범위하고 다양한 출처로부터 얻어질 수 있다. 조합(combinatorial) 라이브러리는 스텝-바이-스텝 방식으로 합성될 수 있는 여러 종류의 화합물로 생산 될 수 있다. 다수의 조합 라이브러리의 화합물들은 ESL(encoded synthetic libraries) 방법(WO 95/12608, WO93/06121, WO 94/08051, WO 95/395503 및 WO 95/30642)에 의해 제조될 수 있다. 펩티드 라이브러리는 파지 디스플레이 방법(WO91/18980)에 의해 제조될 수 있다. 박테리아, 곰팡이, 식물 및 동물 추출물 형태의 자연 화합물의 라이브러리는 상업적인 출처로부터 얻거나 또는 필드(field)에서 수집될 수 있다. 공지된 약리학적(pharmacological) 제제가 구조적 아날로그를 제조하기 위하여 아실화, 알킬화, 에스테르화 반응(esterification), 아미드화 반응(amidification)과 같은 지시되거나(direct) 무작위한 화학적 수식에 적용될 수 있다.
상기 시험 제제는 자연적으로 생성되는 단백질 또는 그의 단편일 수 있다. 이런 시험 제제는 자연 출처(natural source), 예컨대, 세포 또는 조직 용해물로부터 수득될 수 있다. 폴리펩티드 제제의 라이브러리는 예컨대, 통상적인 방법에 의해 생성되거나 상업적으로 입수할 수 있는 cDNA 라이브러리로부터 수득될 수 있다.상기 시험 제제는 펩티드, 예컨대, 약 5-30개, 바람직하게는 약 5-20개, 보다 바람직하게는 약 7-15개의 아미노산을 가지는 펩티드일 수 있다. 상기 펩티드는 자연적으로 생성되는 단백질, 랜덤 펩티드 또는 "바이어스(biased)" 랜덤 펩티드의 절단물일 수 있다.
또한 상기 시험 제제는 "핵산"일 수 있다. 핵산 시험 제제는 자연적으로 생성되는 핵산, 랜덤 핵산, 또는 "바이어스(biased)" 랜덤 핵산일 수 있다. 예컨대, 원핵 또는 진핵 게놈의 절단물을 위에서 기재한 바와 유사하게 사용될 수 있다.
또한 상기 시험 제제는 소분자(예: 약 1,000 이하의 분자량을 갖는 분자)일 수 있다. 소분자의 조절 제제를 스크리닝하기 위한 방법에는 바람직하게는 고속 분석 어세이(high throughput assay)가 적용될 수 있다. 많은 어세이가 상기 스크리닝에 유용하다(Shultz, Bioorg. Med. Chem. Lett., 8:2409-2414, 1998; Weller, Mol.Drivers., 3:61-70, 1997; Fernandes, Curr. Opin. Chem. Biol., 2:597-603, 1998; and Sittampalam, Curr.Opin. Chem. Biol., 1:384-91, 1997).
본 발명의 방법에 스크리닝되는 시험 제제의 라이브러리는 Snail 1과 EPRS 또는 이의 단편이나 아날로그에 대한 구조 연구를 근거로 제조될 수 있다. 이런 구조 연구는 Snail 1 또는 EPRS에 결합할 가능성이 있는 시험 제제의 규명을 가능하게 한다. Snail 1 또는 EPRS의 3차원적 구조는 여러 방법, 예컨대, 결정학 구조 및 분자적 모델링(crystal structure and molecular modeling)으로 연구될 수 있다. X-선 결정학(X-ray crystallography)을 이용하는 단백질 구조 연구 방법이 문헌에 잘 알려져 있다: Physical Bio-Chemistry, Van Holde, K. E.(Prentice-Hall, New Jersey 1971), pp.221-239, and Physical Chemistry with Applications to the Life Sciences, D. Eisengerg ＆ D.Crothers(Benjamin Cummings, Menlo Park 1979). Snail 1 또는 EPRS의 구조에 대한 컴퓨터 모델링은 스크리닝하기 위해 시험 제제의 디자인을 위한 다른 수단을 제공한다. 분자적 모델링 방법은 문헌에 개시되어 있다: U.S. Pat. No. 612,894 and U.S. Pat. No. 5,583,973. 또한 단백질 구조는 중성자 회절(neutron diffraction) 및 NMR(nuclear magnetic resonance)에 의해 결정될 수 있다: Physical Chemistry, 4th Ed. Moore, W. J.(Prentice-Hall, New Jersey 1972) and NMR of Proteins and Nucleic Acids, K. Wuthrich(Wiley-Interscience, New York 1986).
본 발명은
(a) EPRS(Glutamyl-prolyl-tRNA synthetase) 전체 또는 서열번호 2 내지 서열번호 6 으로 이루어지는 군에서 선택된 어느 하나의 아미노산 서열을 가지는 이의 단편, snail 1 단백질 및 시험제제를 접촉시키는 단계; 및
(b) 상기 EPRS와 snail 1의 결합수준의 변화를 측정하는 단계를 포함하는 EMT(epithelial-mesenchymal transition) 저해제 스크리닝 방법을 제공한다.
본 명세서에서 용어 “Snail 1(스네일 1)”은 인간 스네일 1 단백질 또는 이와 동등한 기능을 갖는 인간 이외 기원(바람직하게 포유류 기원)의 상동성(homologous) 스네일 1 단백질을 포함한다. 예를들어 서열번호 15로 표시되는 인간 스네일 1 단백질 일 수 있으나, 이에 제한되지 않는다. 또한 본 발명에서 상기 Snail 1은 이의 기능적 동등물을 포함한다.
상기 Snail 1을 암호화하는 폴리뉴클레오티드는 서열번호 15로 표시되는 아미노산 서열 또는 이와 적어도 70% 이상의 서열 상동성을 갖는 아미노산 서열을 암호화하는 염기서열을 가질 수 있다. 바람직하게 서열번호 16으로 표시되는 염기서열을 갖는 것일 수 있다. 상기 핵산은 천연에서 분리되거나 위에서 기술한 바와 같이 유전공학적인 방법에 의해 제조될 수 있다.
상기 Snail 1의 기능적 동등물은, Snail 1의 아미노산 서열과 적어도 70％ 이상, 바람직하게는 80％ 이상, 보다 바람직하게는 90％ 이상의 서열 상동성(즉, 동일성)을 갖는 폴리펩티드를 말한다. 구체적으로 서열번호 15의 Sainl 1 아미노산 서열과 99% 상동서열인 NCBI Gene Bank Accession Number AAD17332.1(서열번호 124), BAG36039.1(서열번호 125), XP_004062397.1(서열번호 126), AAF32527.1(서열번호 127); 98% 상동서열인 NCBI Gene Bank Accession Number XP_003809303.1(서열번호 128), XP_003252972.1(서열번호 129), XP_010382148.1(서열번호 130), XP_001097698.1(서열번호 131), XP_002830458.1(서열번호 132), XP_009435687.1(서열번호 133); 97% 상동서열인 NCBI Gene Bank Accession Number XP_003932616.1(서열번호 134); 95% 상동서열인 NCBI Gene Bank Accession Number XP_003787739.1(서열번호 135); 93% 상동서열인 NCBI Gene Bank Accession Number XP_008060134.1(서열번호 136); 92% 상동서열인 NCBI Gene Bank Accession Number XP_008570069.1(서열번호 137), XP_004883645.1(서열번호 138), XP_003732892.1(서열번호 139); 91% 상동서열인 NCBI Gene Bank Accession Number KFO35879.1(서열번호 140); 90% 상동서열인 NCBI Gene Bank Accession Number XP_003467769.1(서열번호 141), XP_006141336.1(서열번호 142), XP_005325275.1(서열번호 143), ELW71708.1(서열번호 144), EFB26680.1(서열번호 145), XP_008696422.1(서열번호 146); 89% 상동서열인 NCBI Gene Bank Accession Number NP_446257.1(서열번호 147), XP_005392380.1(서열번호 148), XP_008833387.1(서열번호 149), XP_005362911.1(서열번호 150), XP_006992689.1(서열번호 151), XP_005688790.1(서열번호 152), XP_005635224.1(서열번호 153), XP_008532217.1(서열번호 154); 88% 상동서열인 NCBI Gene Bank Accession Number XP_002913027.1(서열번호 155), XP_004687529.1(서열번호 156), XP_004412574.1(서열번호 157), XP_007185370.1(서열번호 158), XP_007446447.1(서열번호 159), XP_007128099.1(서열번호 160), XP_004282941.1(서열번호 161), NP_035557.1(서열번호 162), XP_004746271.1(서열번호 163), XP_006206723.1(서열번호 164), XP_006175044.1(서열번호 165), NP_001106179.1(서열번호 166), XP_004430338.1(서열번호 167), XP_005074485.1(서열번호 168), XP_007608553.1(서열번호 169); 87% 상동서열인 NCBI Gene Bank Accession Number XP_005983433.1(서열번호 170), CAA47675.1(서열번호 171), XP_003983440.1(서열번호 172), XP_001501267.2(서열번호 173), XP_003502520.1(서열번호 174), XP_004014930.1(서열번호 175); 86% 상동서열인 NCBI Gene Bank Accession Number XP_004663926.1(서열번호 176), XP_006758858.1(서열번호 177), XP_006098041.1(서열번호 178), XP_007936620.1(서열번호 179), XP_004462255.1(서열번호 180), XP_008139391.1(서열번호 181), XP_004636165.1(서열번호 182), XP_006921969.1(서열번호 183), XP_004370603.1(서열번호 184), XP_004618816.1(서열번호 185), XP_004325726.1(서열번호 186); 85% 상동서열인 NCBI Gene Bank Accession Number XP_007533425.1(서열번호 187), XP_003419974.1(서열번호 188), XP_005574198.1(서열번호 189), AAQ21376.1(서열번호 190), XP_007641286.1(서열번호 191); 84% 상동서열인 NCBI Gene Bank Accession Number XP_005866258.1(서열번호 192), XP_006839389.1(서열번호 193), XP_004698375.1(서열번호 194), XP_006881815.1(서열번호 195), EHH21632.1(서열번호 196); 83% 상동서열인 NCBI Gene Bank Accession Number XP_005860983.1(서열번호 197); 81% 상동서열인 NCBI Gene Bank Accession Number XP_005658936.1(서열번호 198), 79% 상동서열인 NCBI Gene Bank Accession Number XP_006099783.1(서열번호 199), EAW70471.1(서열번호 200), XP_004585952.1(서열번호 201), 78% 상동서열인 NCBI Gene Bank Accession Number XP_004033198.1(서열번호 202), 70% 상동서열인 NCBI Gene Bank Accession Number XP_004451686.1(서열번호 203) 등을 포함하며, 이에 제한되지 않는다.
본 명세서에서 용어“EMT(epithelial-mesenchymal transition)”는 상피 세포의 중간엽 세포로의 전환 현상 및 질환을 의미한다. 상기 EMT는 조직 섬유화, 암의 발생 등등 많은 질병과의 연관성이 밝혀졌다. 예를들어 세포, 특히 암세포는 초기 과정인 EMT를 거쳐 이동성을 획득하고, 주변의 조직에 침투함이 알려져 있다. 이는 EMT 프로세스를 억제하면 초기에 상기 조직 섬유화, 암 등의 많은 EMT 관련 질병에 대한 예방 및 치료가 가능함을 시사한다.
본 명세서에서 용어 “EMT 저해제”는 공지의 EMT 유도성 질환 또는 증상에 대한 예방 및/또는 치료제이며, 구체적으로 암 전이(cancer metastasis), 섬유성 질환(fibrotic disease), 신생혈관형성(angiogenesis), 당뇨병성 신증(diabetic renal nephropathy), 동종이식편 기능장애(allograft dysfunction), 백내장(cataract) 및 심장판막형성 결함(defects in cardiac valve formation)으로 이루어진 군에서 선택된 질환 또는 증상의 예방 또는 치료제일 수 있으나, 이에 제한되지 않는다.
구체적으로, 상기‘암 전이’에서 암의 종류는 예를 들어, 비소세포 폐암, 소세포 폐암, 흑색종, 백혈병, 대장암, 간암, 위암, 식도암, 췌장암, 담낭암, 신장암, 방광암, 전립선암, 고환암, 자궁경부암, 자궁내막암, 융모암, 난소암, 유방암, 갑상선암, 뇌암, 두경부암, 피부암, 림프종, 재생불량성 빈혈, 담도암, 구강암, 복막암, 소장암 및 안종양로 이루어진 군에서 선택되는 것일 수 있으나, 이에 제한되지 않는다.
또한 상기 섬유증은 예를 들어 신장 섬유증, 간 섬유증, 폐 섬유증, 피부 섬유증, 심장 섬유증, 관절 섬유증, 신경 섬유증, 근 섬유증 및 복막 섬유증으로 이루어진 군에서 선택되는 것일 수 있으나, 이에 제한되지 않는다.
본 발명의 EMT 저해제는 바람직하게 암(특히, 암 전이)의 예방 또는 치료제 일 수 있고, 가장 바람직하게 비소세포폐암(특히 비소세포폐암 전이)의 예방 또는 치료제일 수 있다.
상기 용어‘결합’은 EPRS 전체 또는 서열번호 2 내지 서열번호 6 으로 이루어지는 군에서 선택된 어느 하나의 아미노산 서열을 가지는 이의 단편과 snail 1 단백질의 직접적 또는 간접적 결합일 수 있다. 상기 간접적 결합은 두 단백질간의 결합이 다른 매개인자를 통하여, 또는 매개인자와 함께 복합체(complex)를 이루는 것을 의미한다.
본 발명에서 상기 EPRS와 snail 1의 결합 수준의 변화는 바람직하게 결합 수준(level)의 감소일 수 있다.
상기 결합수준의 감소는 EPRS와 snail 1의 결합을 제거, 방지, 또는 억제하는 것을 의미한다. 구체적으로, 상기 결합수준의 감소는 시험제제가 EPRS 또는 Snail 1을 제거, 생성 방지 또는 생성 억제하여 EPRS 또는 Snail 1의 발현된 수준을 변화시키거나, 또는 시험제제가 EPRS 또는 Snail 1에 경쟁적 또는 비경쟁적으로 결합하여 둘 간의 상호작용(결합) 수준을 변화시키는 방법, 또는 이미 세포내에 생성된 EPRS-Snail1 단백질 복합체에 대하여 시험제제에 의해 상기 복합체의 EPRS 및 Snail 1 사이의 상호작용(결합)을 제거하는 방법 등에 의하여 이루어질 수 있다. 상기 경쟁적으로 결합하는 것은 EPRS 또는 Snail 1이 서로 상호작용(결합)하는 부위에 시험제제가 결합하여 EPRS와 Snail 1의 상호작용을 제거, 방지, 또는 억제하는 것을 의미하며, 비경쟁적으로 결합하는 것은 EPRS 또는 Snail 1이 서로 상호작용(결합)하는 부위 외의 부위에 시험제제가 결합하여 EPRS와 Snail 1의 상호작용을 제거, 방지 또는 억제하는 것을 말한다. 즉, 본원 발명은 부작용을 일으키지 않는 범위 내에서, EPRS 또는 Snail 1의 발현 및 본연의 기능들을 억제함과 동시에 (혹은 비의존적으로) Snail 1 과 EPRS의 세포내 상호작용(결합)수준을 억제 또는 감소시키는 시험제제를 스크리닝하는 것이다.
바람직하게 본 발명의 결합수준의 감소는 바람직하게 시험제제가 EPRS 또는 Snail 1에 경쟁적 또는 비경쟁적으로 결합하여 둘 간의 상호작용(결합) 수준을 변화시키는 방법, 또는 이미 세포내에 생성된 EPRS-Snail1 단백질 복합체에 대하여 시험제제에 의해 상기 복합체의 EPRS 및 Snail 1 사이의 상호작용(결합)을 제거되는 방법으로 이루어질 수 있다.
상기 시험제제들은 반드시 EPRS 또는 Snail 1의 발현 및 본연의 기능들을 기능적으로 억제할 필요는 없으며, EPRS 와 Snail 1 과의 상호작용(결합)을 억제하는 정도면 충분하다.
상기 본 발명의 스크리닝 방법은 표지된 시험관 내 단백질-단백질 결합 어세이(시험관 내 풀-다운 어세이), EMSA,단백질 결합을 위한 면역어세이, 기능적 어세이(인산화 어세이 등), 효모-2 하이브리드 어세이, 비면역침전 어세이, 면역 침전 웨스턴 블럿 어세이, 면역-공동-위치화 어세이 등 당업계에 공지된 다양한 방법으로 수행될 수 있으며, 상기한 바에 의해 제한되지 않는다.
예컨대, 박테리아 리프레서 LexA 또는 효모 GAL4의 DNA-결합 도메인 및 효모 GAL4 단백질의 트랜스엑티베이션(transactivaton) 도메인에 각각 융합된, EPRS와 Snail 1 또는 이들 단백질의 일부 또는 상동체(homologues)를 발현하는 효모를 이용하여 효모-2 하이브리드 어세이를 수행할 수 있다(KIM, M. J. et al., Nat. Gent., 34:330-336, 2003). EPRS와 Snail 1의 상호작용은 LexA 단백질 또는 GAL4의 DNA-결합 도메인에 결합된 조절 서열을 가지고 있는 프로모터의 통제 하에서 리포터 유전자의 발현을 유도하는 트랜스엑티베이터를 재구성하게 한다.
상기 리포터 유전자로는 위에서 기재한 바와 같이 당업계에 공지된 임의의 검출가능한 폴리펩티드를 암호화하는 유전자(예: CAT(chloramphenicol acetyltransferase), 루시퍼라제, 베타-갈락토시다제, 베타-글루코시타제, 알칼린 포스파타제 및 GFP(green fluorescent protein) 등 를 사용할 수 있다. 만약 EPRS와 Snail 1 또는 이들 단백질의 일부 또는 상동체의 결합이 시험 제제에 의해 저해되거나 약화되는 경우, 상기 리포터 유전자는 발현되지 않거나 정상 조건에 비해 덜 발현된다.
또한 리포터 유전자로는 효모의 성장을 가능하게 하는 단백질(즉, 상기 리포터 유전자가 발현되지 않을 때 효모의 성장이 억제된다)을 암호화하는 것으로 선택될 수 있다. 예를 들면, 아미노산 또는 질소 염기를 위한 생합성 과정에 관계있는 효소를 암호화하는 영양요구성(auxotropic) 유전자(예: ADE3, HIS3 등의 효모 유전자 또는 다른 종 유래의 동등 유전자)일 수 있다. 이 시스템에서 발현된 EPRS와 Snail 1 또는 이들 단백질의 일부 또는 상동체의 결합은 시험 제제에 의해 저해되는 경우, 리포터 유전자는 발현되지 않는다. 따라서, 상기 조건 하에서 효모의 성장은 정지되거나 느려진다. 이러한 리포터 유전자의 발현에 의한 효과는 육안이나 장치(예: 현미경)를 통하여 관찰될 수 있다.
상기 (a) 및 (b) 단계 후에 추가적으로 하기의 (c) 단계를 수행할 수 있다; (c) 상기 (b) 단계에서 EPRS와 snail 1의 결합수준이 변화된 시험제제를 TGF-β1과 함께 snail 1을 발현하는 세포와 접촉시키고 상기 세포에서 EMT 억제 효과를 확인하는 단계;
상기 TGF-β1은 세포 상피 표현형에서 침투성 중간엽 표현형으로의 변이 즉, EMT를 유도하는 것으로 알려져 있다. 상기 Snail 1은 EMT 표현형과의 관련성이 알려져 있으며, 또한 TGF-β1의 처리에 의해 세포내 존재량(발현량)이 증가된다(실시예 1). 따라서 상기 (C) 단계에서는 Snail 1을 발현하는 임의의 세포(특히 정상 상피세포 등)에 TGF-β1을 처리하여 EMT를 유발하고, 여기에 상기 (b) 단계를 통해 일차적으로 선별된 시험제제가 실제 EMT 증상에 있어 치료 효능이 있는지를 확인하는 추가적 공정을 수행한다.
본 발명에서 EPRS는 Snail 1과 상호작용(결합)하여 EMT 경로를 통해 암, 섬유증 등의 기타 EMT 관련 질환을 촉발시키므로, (b) 단계에서 EPRS와 Snail 1의 결합수준을 변화시킨 시험제제는 EMT를 억제할 수 있다.
상기 snail 1을 발현하는 세포는 공지의 Snail 1 발현 세포라면 그 종류가 특별히 제한되지 않으나, 예를 들어 상피세포, 암세포 및 섬유증 세포 등 일 수 있다. 상기 상피 세포(정상 상피세포)는 동물 몸 표면이나 몸속의 빈 공간 또는 관(내장관 등)의 내면을 덮고 있는 세포를 의미하는 것으로서, 망막, 대장, 소장, 혈관, 피부 등등 세포가 유래되는 부위(기관)는 특별히 제한되지 않는다.
상기 암세포로는 예를 들어 비소세포 폐암 세포, 소세포 폐암 세포, 흑색종 세포, 백혈병 세포, 대장암 세포, 간암 세포, 위암 세포, 식도암 세포, 췌장암 세포, 담낭암 세포, 신장암 세포, 방광암 세포, 전립선암 세포, 고환암 세포, 자궁경부암 세포, 자궁내막암 세포, 융모암 세포, 난소암 세포, 유방암 세포, 갑상선암 세포, 뇌암 세포, 두경부암 세포, 피부암 세포, 림프종 세포, 재생불량성 빈혈 세포, 담도암 세포, 구강암 세포, 복막암 세포, 소장암 세포 및 안종양 세포로 이루어지는 군에서 선택되는 것 또는 이들로부터 유래 또는 분화된 세포일 수 있으며, 이에 제한되지 않는다.
상기 섬유증 세포로는 예를 들어 신장 섬유증 세포, 간 섬유증 세포, 폐 섬유증 세포, 피부 섬유증 세포, 심장 섬유증 세포, 관절 섬유증 세포, 신경 섬유증 세포, 근 섬유증 세포 및 복막 섬유증 세포로 이루어지는 군에서 선택되는 것 또는 이들로부터 유래 또는 분화된 세포일 수 있으며, 이에 제한되지 않는다.
상기‘EMT 억제 효과 확인’은 시험제제가 TGF-β1의 처리에 의해 세포에서 촉발되는 EMT 진행현상을 억제하는 지를 확인하는 것으로, 바람직하게 시험제제는 처리되지 않은 채 TGF-β1가 처리된 snail 1을 발현하는 세포를 대조군으로 하여 비교함으로서 이루어지는 것일 수 있다. 상기 EMT 억제 효과 확인 방법은 예를 들어, 공지의 EMT 마커들의 발현 수준을 확인하거나, 또는 세포 이동성(cell migration), 세포 침투성과 같은 EMT에 의해 나타나는 세포 표현형이 나타나는 지를 공지의 방법으로 확인(예를 들어 cell migration assay, cell invasion assay)하는 것일 수 있으며, 이에 제한되지 않는다.
상기 EMT 마커란, 정상상태와 비교하여 EMT 가 진행되는 동안에 특이적으로 변화된 발현을 보이는 단백질을 의미하는 것으로, 공지의 EMT 마커라면 그 종류가 제한되지 않으나 바람직하게 예를들어, p-SMAD3, SMAD2/3, SLUG, E-cadherin, N-cadherin 등으로 이루어진 군에서 선택된 하나 이상의 마커 단백질의 변화를 확인하는 것일 수 있다.
본 발명의 스크리닝 방법은 당업계에 공지된 다양한 생화학적 및 분자생물학적 기술이 상기 방법을 실시하는데 이용될 수 있다. 상기 기술은 다음의 문헌에 개시되어 있다: Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, N.Y., Second(1998) and Third(2000) Editions; and Ausubel et al., Current Protocols in Molecular Biology, John Wiley ＆ Sons, Inc., New York(1987-1999).
바람직하게는 본 발명의 상기 (a) 및 (b), 또는 (a) 내지 (c) 단계를 포함하는 스크리닝 방법에 앞서, 상기 시험 제제가 먼저 EPRS 또는 Snail 1의 생물학적 활성을 조절하는 능력이 있는지 선택적으로 어세이할 수 있다(1차 어세이 단계). 구체적으로 1차 단계에서는 시험 제제의 존재 하에서 분리된 EPRS 또는 Snail 1의 생물학적 활성을 어세이(어세이)하여 상기 폴리펩티드의 생물학적 활성을 조절하는 조절 제제(modulating agent)를 규명(identify)한다. 보다 바람직하게는 다음의 단계를 포함할 수 있다:
(i) 시험 제제의 존재하에서 EPRS 또는 Snail 1과 시험 제제를 접촉시키는 단계;
(ii) EPRS 또는 Snail 1의 활성을 측정하여 이의 활성을 변화시키는 시험 제제를 선별하는 단계;
상기 1차 어세이 단계에서는 EPRS 또는 Snail 1의 여러 생물학적 활성에 대한 조절이 어세이될 수 있다. 예컨대, 시험 제제는 발현 수준, 예컨대, 전사 또는 번역을 조절하는 활성이 있는지에 대하여 어세이될 수 있다. 또한 상기 시험 제제는 세포 내 수준 또는 안정성, 예컨대 번역 후 수식(post-translational modification) 또는 가수분해를 조절하는 활성이 있는지에 대하여 어세이될 수 있다.
상기 1차 어세이 단계를 통하여 생물학적 활성을 변화시키는 조절 제제를 규명한 후, 상기 시험 제제가 EPRS와 Snail 1의 상호작용을 변화시키는 능력이 있는지를 본 발명의 상기 상기 (a) 및 (b), 또는 (a) 내지 (c) 단계를 포함하는 스크리닝 방법으로 테스트한다(2차 어세이 단계).
상기 1차 및 2차 단계에서 모두, 변하지 않은(intact) EPRS 및 이의 단편, 아날로그, 또는 기능적 동등물을 사용할 수 있다. 이들 어세이에 사용될 수 있는 단편은 일반적으로 EPRS 의 생물학적 활성을 하나 이상 보유한다. 또한 상기 단편 또는 아날로그를 포함하는 융합 단백질이 시험 제제를 위한 스크리닝에 사용될 수 있다. EPRS의 기능적 동등물은 아미노산 결실 및/또는 삽입 및/또는 치환을 가지기는 하나 EPRS와 동일한 생체활성을 보유하는 것이므로 본 발명의 스크리닝 방법을 실시하는데 사용될 수 있으며, 이는 전술한 바와 같다.
당업계에 통상적으로 실시되고 있는 다양한 어세이들이 EPRS 또는 Snail 1, 이들의 상호작용를 조절하는 제제를 규명하는데 사용될 수 있다. 바람직하게, 상기 제제는 세포 기반 어세이 시스템(cell based 어세이 system)으로 스크리닝될 수 있다. 예컨대, 스크리닝하기 위한 전형적인 세포 기반 어세이에서(즉, 2차 시험 단계에서), 시험제제의 존재 하에서 리포터 유전자 활성(예: 효소 활성)을 측정하고, 이를 시험 제제의 부재 하에서의 리포터 유전자의 활성과 비교한다. 상기 리포터 유전자는 당업계에 공지된 임의의 검출가능한 폴리펩티드(반응 또는 리포터 폴리펩티드), 예컨대, 형광 또는 인광(phosphorescence)에 의해 검출가능한 폴리펩티드나 그것이 보유하고 있는 효소 활성에 의해 검출가능한 폴리펩티드를 암호화할 수 있다. 검출가능한 반응 폴리펩티드(detectable reponse polypeptide)는, 예컨대, 루시퍼라제, 알파-글루쿠로니다제(glucuronidase), 알파-갈락토시다제, 클로람페니콜 아세틸 전이효소, 녹색 형광 단백질, 강화된 녹색 형광 단백질 및 인간 분비된 알칼린 인산화효소일수 있다.
세포-기반 어세이에서, 시험 제제(예: 펩티드 또는 폴리펩티드)는 숙주세포 내에 존재하는 다른 벡터에 의해 발현될 수 있다. 어떤 방법에서는, 시험 제제의 라이브러리가 상기 벡터의 라이브러리(예: cDNA 라이브러리)에 의해 암호화된다. 상기 라이브러리는 당업계에 공지된 방법을 이용하여 제조할 수 있으며(Sambrook et al. andAusubel et al., supra), 또는 다양한 상업적인 출처로부터 얻어질 수 있다.
상기 기재한 세포-기반 어세이 이외에, 비-세포 기반 방법에 의해서도 스크리닝될 수 있다. 상기 방법은, 예컨대, 모빌리티 쉬프트 DNA 결합 어세이(mobility shift DNA-binding assay), 메틸화 및 우라실 간섭 어세이(methylation and uracil interference assay), DNase 및 히드록시 라디칼 풋프린팅 분석(DNase and hydroxylradical footprinting analysis), 형광 편광(fluorescence polarization) 및 UV 교차결합(crosslinking) 또는 화학적 가교제(cross-linkers)를 포함한다. 일반적인 개요는 Ausubel 등의 문헌에 개시되어 있다(Ausubel et al., supra, chapter 12, DNA-Protein Interaction). 핵산 및 DNA/RNA 결합 단백질을 포함하는 공동-결합된 단백질(co-associating protein)을 분리하는 기술은, 절단 가능한 가교제 디티오비스(dithiobis)(succinimidylpropionate) 및 3,3'-디티오비스(sulfosuccinimidyl-propionate)를 포함하는 UV 교차 결합 또는 화학적 가교제를 포함한다(McLaughlin, Am. J. Hum. Genet., 59:561-569, 1996; Tang,Biochemistry, 35:8216-8225, 1996; Lingner, Proc. Natl. Acad. Sci. U.S.A., 93:10712, 1996; and Chodosh, Mol. Cell. Biol., 6:4723-4733, 1986).
이하에서, 상기 1차 어세이 및 2차 어세이에 대하여 상세히 서술한다.
상기 1차 어세이는 EPRS 또는 Snail 1에 결합, 또는 이를 조절하는 제제를 스크리닝하는 것으로, 본 과정은 당업자의 필요에 따라 선택적으로 사용 될 수 있다. 이하 EPRS로 예를 들어 설명하면, 구체적으로 1차 어세이에서 EPRS에 대한 시험 제제의 결합은, 예컨대 표지된 시험관 내 단백질-단백질 결합 어세이, EMSA(electrophoretic mobility shift assays), 단백질 결합을 검출하기 위한 면역어세이, 기능적 어세이(인산화 어세이 등) 등과 같은 다양한 방법으로 어세이될 수 있다(U.S. Pat. Nos. 4,366,241:4,376,110; 4,517,288 and 4,837,168; and Bevan et al., Trends in Biotechnology, 13:115-122, 1995; Ecker et al., Bio/Technology, 13:351-360, 1995; and Hodgson, Bio/Technology, 10:973-980, 1992). 시험 제제는 EPRS와의 직접적인 결합, 예컨대, EPRS에 대한 항체에 의한 EPRS 폴리펩티드(또는 단백질)와의 공동-침전(co-immunoprecipitation)을 검출함으로써 확인될 수 있다. 또한 시험 제제는 EPRS와 시험 제제의 결합을 나타낼 수 있는 시그널, 예컨대, 형광 퀀칭(quenching)을 검출함으로써 확인될 수 있다.
경쟁 어세이(competition assays)는 EPRS에 특이적으로 결합하는 시험 제제를 규명하는데 적당한 포맷(format)을 제공한다. 상기 포맷에서, 시험 제제는 EPRS와 결합하는 것으로 이미 공지된 화합물과의 경쟁을 통해 스크리닝된다. 공지된 결합 화합물은 합성 화합물일 수 있다. 또한 그것은 EPRS를 특이적으로 인식하는 항체, 예컨대, EPRS에 대한 단일클론 항체일 수 있다. 만일 시험 제제가 EPRS와 상기 공지된 화합물의 결합을 저해한다면, 상기 시험 제제는 EPRS에 결합할 수 있다. 다양한 종류의 경쟁 어세이들이 당업계에 공지되어 있다. 예컨대, 고체상 직접 또는 간접적 효소 방사면역측정법(solid phase direct or indirect radioimmunoassay, RIA), 고체상 직접 또는 간접적 효소 면역측정법(EIA), 샌드위치 경쟁 어세이(Stahli et al., Methods in Enzymology, 9:242-2453, 1983); 고체상 직접 바이오틴-아비틴 EIA(Kirkland et al., J. Immunol., 137:3614-3619, 1986); 고체상 직접 표지화된 어세이, 고체상 직접 표지화된 샌드위치 어세이(Harlow and Lane, Antibodies, A laboratory Manual, Cold Spring Harbor Press, 1988); 125I를 이용한 고체상 직접 표지 RIA(Morel et al., Mol. Immuno., 25(1);7-15, 1988; 고체상 직접 바이오틴-아비틴 EIA(Cheung et al., Virology, 176:546-552, 1990); 및 직접 표지화된 RIA(Moldenhauer et al., Sacnd. J. Immunol., 32:77-82, 1990). 일반적으로 상기 어세이들은 비표지화된 시험 제제 및 표지화된 대조 화합물을 포함하고 있는 세포 또는 고체 표면에 결합되어 있는 정제된 폴리펩티드의 이용을 포함한다. 경쟁적 저해는 시험 제제의 존재 하에서 고체 표면 또는 세포에 결합된 표지의 양을 결정함으로써 측정된다. 경쟁 어세이에 의해 규명된 조절 제제는 대조 화합물과 동일한 에피토프에 결합하는 제제 및 입체 저해(steric hindrance)가 일어나도록 하기 위해 대조 화합물에 의해 결합된 에피토프에 충분하게 근접한 인접 에피토프와 결합하는 제제를 포함한다. 통상적으로 경쟁 저해가 과도하게 존재할 때, 일반적인 표적 폴리펩티드에 대한 대조 화합물의 특이적인 결합이 적어도 50 또는 75％ 저해될 것이다.
상기 스크리닝 어세이는 불용성 또는 용해성 포맷일 수 있다. 불용성 어세이의 일 예는 EPRS 또는 이의 단편을 고체 상 매트릭스에서 고정화하는 것이다. 이후, 시험 제제가 결합하기에 충분한 시간 동안 고체상 매트릭스를 시험 제제와 접촉하여 둔다. 이후, 고체상 매트릭스로부터 결합되지 않은 물질을 세척하여 제거한 후, 고체상에 결합된 제제의 존재를 확인한다. 상기 방법은 고체상 매트릭스로부터 결합된 제제를 용리하여 제제를 분리하는 단계를 더욱 포함할 수 있다. 양자택일적으로, EPRS를 고정화시키는 다른 방법은 시험 제제를 고체상 매트릭스에 결합시킨 후, EPRS를 첨가하는 것이다.
용해성 어세이는 위에 기재된 몇몇 결합 라이브러리 스크리닝 방법을 포함한다. 용해성 어세이 포맷하에서, 시험 제제나 EPRS는 모두 고체 지지대에 결합하지 않는다. 시험 제제에 대한 EPRS 또는 이의 단편의 결합은 예컨대, EPRS 및/또는 시험 제제의 형광에 의해 측정될 수 있다. 형광은 내재적이거나(intrinsic) 형광물질(fluorophor)을 가진 성분의 표지에 의해 부여될 수 있다.
몇몇 결합 어세이에서, EPRS, 시험 제제 또는 제3의 물질(예: EPRS에 결합하는 항체)은, 주어진 조건에서 상기 폴리펩티드의 확인, 검출 및 정량을 용이하게 하기 위하여, 표지된 상태로 제공될 수 있다. 즉, 공유적으로 결합 또는 검출가능한 표지나 그룹, 또는 가교가능한 그룹에 링크되어 제공될 수 있다. 이들 검출가능한 그룹들은 검출가능한 폴리펩티드 그룹, 예컨대, 어세이 가능한(assayable) 효소 또는 항체 에피토프를 포함한다. 양자택일적으로, 상기 검출가능한 그룹은 방사성동위원소(예: 125I, 32P, 35S) 또는 화학발광성 또는 형광성 그룹과 같은 다른 검출가능한 그룹 또는 라벨에서 선택될 수 있다. 유사하게, 상기 검출가능한 그룹은 기질(substrate), 보조인자(cofactor), 저해제 또는 친화 리간드일 수 있다.
상기 1차 어세이에서 EPRS와 시험 제제의 결합은 시험 제제가 EPRS의 조절자임을 나타낸다. 또한 이는 상기 제제가 Snail, 바람직하게는 Snail 1의 생체활성을 조절할 수 있음을 제시한다. 따라서, EPRS와 결합하는 시험 제제는 Snail 1 활성을 조절하는 능력을 가지고 있는지 더욱 테스트되어야 한다.
양자택일적으로, EPRS와 결합하는 시험 제제는 EPRS에 대한 그의 활성을 측정하기 위하여 더욱 조사될 수 있다. 그런 활성의 존재, 성질 또는 범위는 활성 어세이에 의해 테스트될 수 있다. 상기 활성 어세이는 EPRS에 대한 시험 제제의 결합이 실제로 EPRS에 대한 조절 활성을 갖는 것을 확인할 수 있다. 매우 흔히, 상기 조절 어세이들은 EPRS의 활성을 조절하는 시험 제제를 규명하기 위하여 독립적으로 사용될 수 있다(즉, EPRS에 결합하는 능력을 어세이하는 첫 단계 없이). 일반적으로 상기 방법들은 EPRS의 생물학적 활성을 테스트하는데 필요한 다른 물질 또는 시약의 존재 또는 부재 하에서 EPRS를 포함하는 샘플에 시험 제제를 첨가하고, EPRS의 생물학적 활성의 변경을 측정하는 것을 포함한다. EPRS의 효소 또는 다른 생물학적 활성을 조절하는 제제를 스크리닝하는 어세이에 더하여, 상기 활성 어세이는 EPRS의 발현 또는 세포 내 수준의 변화를 위한 시험관 내 스크리닝 및 생체 내 스크리닝을 포함한다.
2차 어세이 단계는 본 발명의 상기 (a) 및 (b), 또는 (a) 내지 (c) 단계의 스크리닝방법을 수행하는 단계를 의미하는 것으로, 상기 1차 어세이에서 일단 시험제제가 EPRS와 결합하거나 및/또는 EPRS의 생물학적 활성(세포 내 수준을 포함)을 조절하는 것으로 규명되면, 상기 제제는 Sanil 1을 통한 EMT 경로의 조절, 더 나아가서는 상기한 EMT 관련(매개) 질환의 예방 또는 치료하는 능력을 가지고 있는지에 대하여 더욱 테스트될 수 있다. 상기 조절 제제에 의한 조절은 일반적으로 EPRS의 존재 하에서 테스트된다. 만약 세포-기반 스크리닝 시스템을 이용하는 경우, EPRS는 숙주세포에 도입된 발현 벡터로부터 발현될 수 있다. 양자택일적으로 EPRS는 스크리닝 시스템에서 숙주세포에 의하여 내생적으로 공급될 수도 있다.
본 발명은 EPRS의 신규한 용도에 관한 것으로, EMT 억제제를 스크리닝하는 방법을 제공한다. 본 발명의 방법은 암을 포함하여 다양한 EMT 관련 질환의 새로운 치료제의 개발에 유용하게 이용될 수 있다.
본 명세서의 전 도면에서 + 기호는 해당 물질의 존재(즉, 트랜스팩션에 의한 발현 및 물질의 처리 상태)를 의미하고 -는 해당물질의 비존재를 의미한다.
도 1은 A549 세포에 TGF-β1을 농도별로 48시간동안 처리하고, 핵과 세포질분획 각각에 대하여 Protein A agarose bead 및 anti-EPRS antibody를 이용해 내생적 면역침강(endogenous immunoprecipitation(IP))을 수행한 후, 이의 웨스턴블롯 결과를 나타낸다(WB/SNAIL: anti-snail 1 antibody로 웨스텃블롯 , WB/EPRS: anti-EPRS antibody로 웨스텃블롯, Cyto:세포질 분획, Nuc: 핵 분획).
도 2는 Flag-tagged EPRS 및/또는 Strep-tagged snail 1이 co-transfection된 HEK293T 세포에서, anti-Flag M2 affinity gel을 사용하여 면역침강(IP)시키고anti-Strep-HRP 및 anti-Flag-HRP antibody로 면역블롯(IB)한 결과를 나타낸다(WCL: whole cell lysate).
도 3은 Strep-tagged snail 1로 일시적 형질전환된 HEK293T 세포에서, snail 1을 MagStrep 2HC로 면역침강(IP)시키고, anti-EPRS, anti-HDAC1, anti-L13a, anti-Strep-HRP antibody로 면역블롯한 결과를 나타낸다(STREP-EV: Strep-tagged empty vector로 형질전환된 HEK293T 세포를 이용한 실험군 , WCL: whole cell lysate).
도 4는 Strep-tagged ERS-WHEP(E로 표시), Strep-tagged WHEP-PRS(P로 표시) 또는 2X Strep-tagged EPRS(EP로 표시)를 Flag tagged-Snail 1과 함께 발현시킨 HEK293T 세포에서, MagStrep 2HC로 면역침강(IP)시키고 anti-Flag-HRP 및 anti-strep-HRP antibody를 이용하여 웨스턴 블롯으로 분석한 결과를 나타낸다(WCL: whole cell lysate).
도 5의 A는 Flag tagged empty vector 또는 Flag-tagged EPRS를 A549 세포에 발현시키고 TGF-β1를 처리 또는 비처리한 후, 각 실험군 별 세포용해물에 대하여 anti-Snail 1, anti-SMAD2, anti-SMAD3, anti-FLAG-HRP, anti-βactin antibody를 사용하여 면역블롯을 수행한 결과를 나타낸다.
도 5의 B는 2X strep-tagged empty vector 또는 2X strep-tagged EPRS를 HCC44 세포에 발현시키고 TGF-β1를 처리 또는 비처리한 후, 각 실험군 별 세포용해물에 대하여 anti-Snail 1, anti-strep-HRP antibody를 사용하여 면역블롯을 수행한 결과를 나타낸다.
도 5의 C는 2X strep-tagged empty vector 또는 2X strep-tagged EPRS를 HEK293T 세포에 발현시키고 TGF-β1를 처리 또는 비처리한 후, 각 실험군 별 세포용해물에 대하여 anti-Snail 1, anti-strep-HRP antibody를 사용하여 면역블롯을 수행한 결과를 나타낸다.
도 5의 D는 2X strep-tagged Mock 또는 2X strep-tagged EPRS를 H1299세포에 서로 다른 양으로 발현시키고, 세포용해물에 대하여 anti-Snail 1, anti-strep-HRP antibody를 사용하여 면역블롯을 수행한 결과를 나타낸다(MOCK: empty vector,
Figure pat00001
: 세포내에서 해당 단백질의 발현량을 증가시킴을 의미).
도 6은 2XStrep-tagged EPRS가 과발현되도록 일시적 형질전환된 A549세포들에 TGF-β1을 처리 또는 비처리한 후, snail(A) 및 EPRS(B)의 mRNA 발현량을 알아보기위해 qRT-PCR assay를 수행한 결과를 나타낸다(MocK: 2XStrep-tagged empty vector plasmid DNA로 형질전환된 A549 세포를 이용한 실험군, EPRS: 2XStrep-tagged EPRS plasmid DNA로 형질전환된 A549 세포를 이용한 실험군, Cont: TGF-β1 비처리군, TGFβ1: TGF-β1 처리군)
도 7은 Strep-tagged ERS, Strep-tagged PRS, Strep-tagged EPRS 각각의 단백질과 Flag-tagged snail 1을 단독으로 또는 함께 HEK293T 세포에서 발현시키고, MG132 처리군 및 비처리군에서 세포 용해물에 대해 anti-Flag-HRP로 면역블롯한 결과를 나타낸다(EV:STREP-tag empty vector와 FLAG-tag empty vector 발현).
도 8은 HA-tagged Ubiquitin을 Strep-snail 1 및, Flag-Mock 또는 Flag-EPRS로 co-transfection하고, 세포를 회수하기 6시간 전에 Proteasome inhibitor인 MG-132를 처리한 후, 이의 용해물을 streptavidin agarose bead(GE healthcare lifesciences) 으로 침전시키고 anti-HA antibody로 면역블롯하여 snail 1의 유비퀴틴화에 대한 EPRS의 영향을 확인한 결과를 나타낸다(WCL: whole cell lysate).
도 9의 A는 EPRS가 넉다운(knockdown)된 A549 세포에 TGF-β1를 1시간 처리 또는 비처리하고, 상기 세포의 용해물을 핵 및 세포질 단백질로 분획하여 snail 1, p-SMAD3, SMAD2/3, SLUG, EPRS의 EMT marker 단백질들에 대하여 면역블롯을 수행한 결과를 나타낸다(si-con: non-silencing control siRNA 도입군, si-EPRS: 인간 EPRS 유전자 특이적인 siRNA를 도입하여 넉다운 유도군, HSP90α/β, p84은 각각 세포질, 핵에서의 단백질 발현양을 확인하는 loading control 로 사용함).
도 9의 B는 EPRS가 넉다운(knockdown)된 A549 세포에 TGF-β1를 48시간 처리 또는 비처리하고, 상기 세포의 용해물을 핵 및 세포질 단백질로 분획하여 E-cadherin, N-cadherin, EPRS의 EMT marker 단백질들에 대하여 면역블롯을 수행한 결과를 나타낸다(si-con: non-silencing control siRNA 도입군, si-EPRS: 인간 EPRS 유전자 특이적인 siRNA를 도입하여 넉다운 유도군, HSP90α/β, p84은 각각 세포질, 핵에서의 단백질 발현양을 확인하는 loading control 로 사용함).
도 10은 EPRS가 넉다운된 A549 세포의 TGF-β1처리군 및 비처리군에서, 세포의 이동성분석결과 중 현미경 확대 이미지를 나타낸다(si-con: non-silencing control siRNA 도입군, si-EPRS: 인간 EPRS 유전자 특이적인 siRNA를 도입하여 넉다운 유도군).
도 11은 EPRS가 넉다운된 A549 세포의 TGF-β1처리군 및 비처리군에서, 세포의 이동성분석 결과를 정량화하여 나타낸것이다(si-con: non-silencing control siRNA 도입군, si-EPRS: 인간 EPRS 유전자 특이적인 siRNA를 도입하여 넉다운 유도군).
도 12는 EPRS가 넉다운된 A549 세포의 TGF-β1처리군 및 비처리군에서, 세포의 침투성(invasion) 분석결과 중 현미경 확대 이미지를 나타낸다(si-con: non-silencing control siRNA 도입군, si-EPRS: 인간 EPRS 유전자 특이적인 siRNA를 도입하여 넉다운 유도군).
도 13은 EPRS가 넉다운된 A549 세포의 TGF-β1처리군 및 비처리군에서, 세포의 침투성(invasion) 분석결과를 정량화하여 나타낸 것이다(si-con: non-silencing control siRNA 도입군, si-EPRS: 인간 EPRS 유전자 특이적인 siRNA를 도입하여 넉다운 유도군).
도 14는 서열번호 1 내지 서열번호 7로 표시되는 폴리펩티드에 대하여, 각각을 구성하는 도메인(domain)을 나타낸 모식도이다( 각각, “EPRS(WT)는 서열번호 1에 해당,“EPRS(△PRS)”는 서열번호 2에 해당, “EPRS(△WHEP, PRS)”는 서열번호 3에 해당, “EPRS(△ERS)”는 서열번호 4에 해당, “EPRS(△ERS,WHEP)”는 서열번호 5에 해당, “EPRS(△WHEP)”는 서열번호 6에 해당, “EPRS(△ERS,PRS)”는 서열번호 7에 해당).
이하 본 발명을 상세히 설명한다.
단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.
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1. 세포 배양 및 재료
하기의 비소세포성암(NSCLC, Non-small cell lung cancer) 세포주와 정상 세포주가 본 실험에 사용되었다: adenocarcinoma (A549, HCC44), large-cell (H1299), embryonic kidney cell line HEK293T cell line.
상기 NSCLC 세포주들은 10% fetal bovine serum 및 1% antibiotic를 포함하는 RPMI 배지에서 배양되었고, HEK293T 세포는 1% antibiotics와 함께 10% fetal bovine serum을 함유시킨 DMEM에서 유지되었다. 일시적 형질전환(transiently transfection)을 위하여 Fugene HD (Roche) 및 Lipofectamine 2000 (Invitrogen)이, siRNA 형질전환을 위해서 Lipofectamine RNAiMAX (Invitrogen) reagent가 제조사의 프로토콜에 따라 사용되었다. 핵 및 세포질 추출물은 Nuclear extract kit(Active motif)를 제조사의 프로토콜에 따라 사용하여 수득하였고 TGF-β1 (10 ng/ml) 은 R&D systems으로부터 구입하였다.
2. Plasmid 및 siRNA
Origene으로부터 인간 EPRS(서열번호 1)를 암호화하는 cDNA를 입수하여, 서열번호 8로 표시되는 상기 EPRS cDNA와 각각 ERS-WHEP domain(서열번호 2), WHEP-PRS domain(서열번호 4)을 암호화하는 서열번호 9, 서열번호 11로 표시되는 cDNA 각각을 Strep tag(목적단백질의 N-말단에 위치)와 함께 pEXPR-IBA-105(iba) 플라스미드에 서브클론(subclone)하였다. 이렇게 생성된 각각의 발현 벡터 pEXPR-2xIBA105-EPRS, pEXPR-IBA105-ERS, pEXPR-IBA105-PRS 들은 각 실험별로 선택적으로 세포에 트랜스 펙션transfection) 되었다. 또한 Origene으로부터 인간 Flag tagged EPRS 발현벡터를 입수하였다.
Snail 1의 발현을 위하여 Addgene으로부터 pCMV-Tag2B N-terminal Flag-tagged snail 1 expression vector를 수득하였다. 또한 상기와 유사한 방법으로 pEXPR-IBA-105 벡터를 이용하여 Strep tagged Snail 발현벡터(pEXPR-IBA105-Snail1)를 제조하였다. 상기 pEXPR-IBA105-Snail1 벡터는 서열번호 16으로 표시되는 DNA 서열을 포함하여, 서열번호 15의 Snail 1 단백질을 발현한다.
대조군으로서 상기 pEXPR-IBA-105에 Strep 또는 Flag만 태그된 각각의 벡터Strep-tag empty vector, Flag-tag empty vector를 제작하여 사용하였다. 또한 HA-tagged ubiquitin 발현벡터는 addgene으로부터 입수하였다.
서열번호 23의 센스 및 서열번호 24의 안티센스 서열을 포함하는 인간 EPRS 유전자 특이적인 siRNA(si-EPRS)와 non-silencing control siRNA(si-control, Catalog Number: 4390843) 는 Invitrogen Silencer Select siRNAs products로 부터 구입하였다.
3. 세포 이동 분석법(Cell migration assay)
A549 세포를 이용한 세포 이동 분석은 Transwell chamber (8.0 μM pore, Costar)에서 수행되었다. Fibronectin (10μg/ml,BD Biosciences)은 멤브레인(membrane)에 코팅되었고, 700μl의 serum-free RPMI은 하부 chamber에 위치되었다. A549 세포들을 트립신화(trypsinize)한 후, 1,000 rpm 으로 5분동안 원심분리하고, 세포에 serum-free RPMI 을 첨가하하여 현탁액을 제조하였다. 그리고나서, 1X105 세포들은 24-well Transwell chamber에 투입되었고, 이들은 CO2 인큐베이터에서 37℃, 12시간동안 배양되었다. 멤브레인을 PBS 로 2회 세척한 후, 70% methyl alcohol 이 포함된 PBS 를 사용하여 세포를 고정하였다. 그 뒤에 PBS로 3회 세척하고, hematoxylin (Sigma) 을 사용하여 세포 염색하였다. 증류수로 몇번 멤브레인을 세척한 후, 상부의 비이동 세포들은 면봉을 이용하여 제거되었다. 그 다음에 상기 멤브레인은 Gel Mount (Biomeda)을 사용하여 마운팅(mounting)되었다. 이동 세포들을 카운팅하기 위하여, 무작위로 선정된 3군데가 high-power fields (X10 , 및 X20)조건에서 현미경으로 확대되었다. 모든 샘플들은 3반복 시험되었다.
4. 면역침강법(Immunoprecipitation, IP)
Strep-tagged EPRS, Strep-tagged ERS-WHEP, Strep-tagged WHEP-PRS 또는/및 Flag-tagged Snail1 등과 같이, Strep-tag된 단백질과 Flag-tag된 단백질들이 일시적으로 co-transfection 된 세포들은 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 10% glycerol 및 protease inhibitor (Calbiochem)를 포함하는 20mM의 Tris-HCl (pH 8.0) buffer 로 용해되었다. Strep-tag된 단백질들을 정제하기 위하여, MagStrep Type 2HC beads (IBA lifesciences)을 제조사의 프로토콜에 따라 사용하였다. Flag-tag된 단백질들을 정제하기 위하여, anti-FLAG M2 affinity gel(Sigma)을 제조사의 프로토콜에 따라 사용하였다.
핵 및 세포질에서 내생성 EPRS(endogenous EPRS)와 snail 1의 상호작용을 검출하기 위하여, 단백질 추출물들은 얼음 위에서 30분동안 protein A agarose bead(Invitrogen)로 배양되었고, 그 후 비특이적 IgG 결합 단백질을 제거하기위하여 원심분리되었다. 상기 전처리된(pre-cleared) 상층액은 anti-EPRS antibody와 혼합되었고, 상기 혼합물을 부드럽게 교반해주며(gentle agitation) 4℃에서 2시간동안 배양하였고, protein A agarose bead을 2시간동안 처리하고 원심분리하였다. 침전물을 차가운 용해버퍼(lysis buffer)로 3~5회 세척한 후, 침전물은 2X SDS sample buffer에 용해되었고 SDS-PAGE에 의해 분리되었다.
5. 유비퀴틴화 분석(Ubiquitination assay)
Snail 1의 유비퀴틴화에 대한 EPRS의 영향을 조사하기 위하여, HA-tagged ubiquitin은 Strep-tagged Snail1 및, Flag-tagged Mock 또는 Flag-tagged EPRS 와 A549 세포에 co-transfaction되었고, 세포를 harvest하기 6시간 전에 50μM 농도의 MG-132를 전 처리하였다. 세포 용해물은 streptavidin agarose bead(GE healthcare lifesciences)로 면역침강되었다. 상기 비드(bead)들은 차가운 워싱버퍼로 3회 세척되었고, 침전물은 2X SDS sample buffer에 용해하고 SDS-PAGE를 수행하였다. 그 다음에 anti-HA antibody(SCBT)로 웨스턴 블롯 분석을 수행하였다.
6. 웨스턴 블롯(면역 블롯, IB) 분석
세포들은 용해버퍼(150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 10% glycerol 및 protease inhibitor를 포함하는 20mM Tris-HCl (pH 8.0) buffer)로 용해되었고, 1.5 ml tube에 모아진 뒤, 15분동안 부드럽게 교반(gentle agitation)해주며 4℃에서 배양되었다. 그리고 나서, 4 ℃ 에서 15,000 rpm 으로 20분동안 원심분리하고 상층액을 모은 후 Bradford reagent를 사용하여 정량하였다. 5× SDS sample buffer를 첨가한 후, 각 샘플들은 100℃에서 5분동안 가열되었다. 단백질 샘플들은 SDS-PAGE에 의해 분리되었고, nitrocellulose membrane로 트랜스퍼(transfer)되었으며, 목적하는 마커단백질에 특이적 항체로 면역블롯되었다. 그 후 상기 면역블롯된 것은 horseradish peroxidase-coupled anti-mouse 및 anti-rabbit (Pierce) IgG secondary antibody와 반응시켰다. 신호는 ECL-PLUS detection reagent (GE healthcare lifesciences)로 증가되었다.
본 실험에 사용된 각 마커 단백질에 특이적인 항체는 하기와 같다; StrepMAB classic-HRP(IBA), Monoclonal anti-FLAG M2-HRP(Sigma), anti-Snail1(Cell signaling), anti-Slug(Cell signaling), anti-L13a(Cell signaling), anti-p-Smad2(Cell signaling), anti-p-Smad3(Cell signaling), anti-E-cadherin(Cell signaling), anti-N-cadherin(Cell signaling), anti-HDAC1(Bio vision), anti-beta-actin(SCBT), anti-Tubulin(Sigma), anti-HSP90 alpha/beta(SCBT), anti-p84(SCBT), anti-HA(SCBT), 그리고 anti-EPRS antibody는 서열번호 1로 표시되는 EPRS 단백질을 뉴질랜드 흰토끼에 주입하여 면역반응을 일으킨 후 이에 대한 항체를 수득하는 통상적인 과정으로 제작하였다.
7. qRT-PCR assay
strep-tag empty vector 또는 strep-tag EPRS 발현 벡터로 트랜스펙션(transfection)된 A549 세포들에대하여 TGF-β1(10ng/ml)을 12시간동안 처리한 군과 비처리군을 준비하였다. 그 후, GeneJET RNA Purification kit (Thermo Scientific)를 사용하여 모든 RNA를 추출하였다. RNA 2㎍을 사용하여 Maxima first strand cDNA synthesis kit for RT-qPCR (Thermo Scientific, #K1641)를 가지고 제조사의 프로토콜에따라 cDNA를 합성하였다. qRT-PCR은 상기 cDNA 템플릿, SYBR green Master MIX with ROX solution (Thermo Scientific)과, 하기 [표 1]에 기재된 정방향 및 역방향 프라이머들을 0.3uM 씩 이용하여 ABI 7500 instrument (life technologies)로 수행되었다. 실행 온도는 denaturation 95℃, primer annealing/polymerase extension 60℃로 수행되었다.
Target direction sequence 서열번호
EPRS Forward 5'-ATCCGCGTTAGAGCTGATTT-3' 17
Reverse 5'-TAATGGGAACTCCCTTGAGC-3' 18
SNAIL1 Forward 5'-CCTCCCTGTCAGATGAGGAC-3' 19
Reverse 5'-CCAGGCTGAGGTATTCCTTG-3' 20
GAPDH Forward 5'-CAATGACCCCTTCATTGACC-3' 21
Reverse 5'-GACAAGCTTCCCGTTCTCAG-3' 22
8. 세포 침투(invasion) 분석
A549 세포를 이용한 세포 침투 분석은 BD사에서 제조된 Matrigel invasion chamber 에서 수행되었다. 제조사의 프로토콜에 따라 matrigel을 두 시간 동안 rehydration 하였고, 750μl의 serum-free RPMI은 하부 chamber에 위치되었다. A549 세포들을 트립신화(trypsinize)한 후, 1,000 rpm 으로 5분동안 원심분리하고, 세포에 serum-free RPMI 을 첨가하여 현탁액을 제조하였다. 그리고나서, 1X105 세포들은 upper chamber에 투입되었고, 이들은 CO2 인큐베이터에서 37℃, 12시간동안 배양되었다. 멤브레인을 PBS 로 2회 세척한 후, 70% methyl alcohol 이 포함된 PBS 를 사용하여 세포를 고정하였다. 그 뒤에 PBS로 3회 세척하고, hematoxylin (Sigma) 을 사용하여 세포 염색하였다. 증류수로 몇번 멤브레인을 세척한 후, 상부의 비이동 세포들은 면봉을 이용하여 제거되었다. 그 다음에 상기 멤브레인은 Gel Mount (Biomeda)을 사용하여 마운팅(mounting)되었다. 이동 세포들을 카운팅하기 위하여, 무작위로 선정된 3군데가 현미경으로 확대되었다. 모든 샘플들은 3반복 시험되었다.
<실시예 1>
핵 EPRS와 Snail 1의 상호작용 확인
<1-1> 암세포의 endogenous level에서 상호작용 확인
EPRS와 Snail1의 상호작용을 세포질과 핵의 endogenous level에서 확인하였다. A549 세포에 TGF-beta1을 각각 다른 농도 (0, 1, 10, 20 ng/ml) 로 48 시간 처리한 후, 세포질과 핵으로 나누어 Protein A agarose bead(Invitrogen)와 EPRS antibody를 이용, endo-IP를 진행하였다.
상기 IP한 시료를 Snail1 antibody를 이용한 면역 블롯으로 확인한 결과, 도 1에서 보는 바와 같이 TGF-beta1의 농도가 높아질수록 세포질과 핵에서 EPRS와 Snail1의 결합이 강해지는 것을 확인하였다.
<1-2> co-transfection에 의한 상호작용 확인
EPRS와 Snail1의 상호작용을, 상기 단백질들의 co-transfection 후 IP를 통해 확인하였다. HEK293T 세포에 (1) FLAG-tag empty vector와 STREP-tag empty vector, (2) FLAG-tag empty vector와 STREP tag된 Snail1 발현벡터, (3) FLAG tag된 EPRS 발현벡터와 STREP-tag empty vector, (4) FLAG tag된 EPRS 발현벡터와 STREP tag된 Snail1 발현벡터의 총 네 가지 조건으로 co-transfection하여 각각의 단백질을 과발현한 후, anti-FLAG M2 affinity gel(Sigma)을 이용하여, IP를 진행하였다.
상기 FLAG IP한 네 가지 조건의 시료를 STREP antibody로 면역 블롯한 결과, 도 2에서 보는 바와 같이 과발현된 EPRS와 Snail1이 상호작용 하는 것을 확인하였다.
<1-3> Snail1의 과발현 조건에서의 상호작용 확인
EPRS와 Snail1의 상호작용을, Snail1의 과발현 후 IP를 통해 확인하였다. HEK293T 세포에 (1) STREP-tag empty vector, (2) STREP tag된 Snail1 발현벡터의 총 두 가지 조건으로 transfection하여 단백질을 과발현한 후, MagSTREP type 2HC bead(IBA)를 이용, IP를 진행하였다.
상기 STREP IP한 두 가지 조건의 시료에서 EPRS antibody로 면역 블롯한 결과를 도 3에서 보여준다. empty vector 과발현 시료에서는 EPRS band가 관찰되지 않고, Snail1 과발현 시료에서는 EPRS band가 관찰되었다. 즉, 과발현 된 Snail1과 EPRS가 상호작용 하는 것을 확인하였다. 이 때, 과발현 된 Snail1의 IP가 잘 되었는 지를 증명하기 위해, Snail1과 상호작용을 한다고 알려진 HDAC1을 함께 면역 블롯 하였다. IFN-r 신호 전달에 의해 EPRS와 함께 GAIT complex를 이루어 translation을 조절한다고 알려진 (Mukhopadhyay, Jia et al. 2009) 구성 성분들도 Snail1과 상호작용을 하는 지 알아보기 위해 그 구성 성분 중 하나인 L13a를 면역 블롯 하였고, 그 결과 Snail1과의 상호작용은 관찰되지 않았다.
<1-4> EPRS 단편과 Snail1의 상호작용 확인
EPRS를 ERS-WHEP domain(서열번호 2), WHEP-PRS domain(서열번호 4), EPRS(서열번호 1)으로 나누어, Snail1과의 상호작용을 co-transfection 후 IP를 통해 확인하였다. HEK293T 세포에 (1) STREP-tag empty vector와 FLAG-tag empty vector, (2) STREP tag된 ERS-WEHP 발현벡터와 FLAG tag된 Snail1 발현벡터, (3) STREP tag된 WHEP-PRS 발현벡터와 FLAG tag된 Snail1 발현벡터, (4) STREP tag된 EPRS 발현벡터와 FLAG tag된 Snail1 벡터 총 네 가지 조건으로 transfection하여 단백질을 과발현한 후, MagSTREP type 2HC를 이용하여 IP를 진행하였다.
STREP IP한 네 가지 조건의 시료를 FLAG antibody로 면역 블롯한 결과 도 4에서 보는 바와 같이, 과발현된 ERS-WHEP, WHEP-PRS, EPRS와 Snail1이 상호작용 하는 것을 확인하였다. 그 중에서도 WHEP-PRS과 Snail1의 상호작용이 ERS-WHEP, EPRS보다 강하게 관찰되었다.
<실시예 2>
물리적 상호작용을 통한 EPRS의 Snail 1 안정성 증가 효과 확인
<2-1> 단백질 수준에서의 영향 확인
EPRS의 과발현이 Snail1의 발현에 어떠한 영향을 주는 지 알아보기 위한 실험으로, 각각 A549, HCC44, 293T 세포에 (1) Empty vector를 과발현 후, TGF-beta1을 처리하지 않은 실험군, (2) Empty vector를 과발현 후, TGF-beta1을 처리한 실험군, (3) EPRS를 과발현 후, TGF-beta1을 처리하지 않은 실험군, (4) EPRS를 과발현 후, TGF-beta1을 처리한 실험군 총 네 가지 조건으로 나누어 실험하였다. 이때 TGF-beta1 처리군은 10ng/ml의 농도로 한 시간 처리되었다. 상기 네 가지 실험군의 세포들은 각각 용해된 후, Snail1 antibody로 면역블롯되었다.
그 결과 도 5의 A,B 및 C에서 보는 바와 같이 A549, HCC44, 293T 세포주에서 모두 EPRS를 과발현하면, TGF-beta1을 처리하지 않아도 Snail1의 발현이 증가하며, TGF-beta1을 처리하면 그 증가율이 더 강해지는 것을 Snail1 antibody로 면역 블롯을 하여 확인하였다.
이 외에도 도 5의 D에서보는 바와 같이 H1299 세포내에서 EPRS의 발현량을 점차 증가 시킬수록, Snail1의 발현 또한 더욱 증가하는 것을 Snail1 antibody로 면역 블롯을 하여 확인하였다 (도 5의 D에서‘
Figure pat00002
’기호는 세포내에서 해당 단백질(즉,EPRS)의 발현량을 증가시킴을 의미).
<2-2> mRNA 발현 수준에서의 확인
EPRS의 과발현이 Snail 1의 mRNA level 변화에 어떠한 영향을 주는 지 알아보기 위한 실험으로, A549 세포에 (1) Empty vector를 과발현 후, TGF-beta1을 처리하지 않은 실험군, (2) Empty vector를 과발현 후, TGF-beta1을 처리한 실험군, (3) EPRS를 과발현 후, TGF-beta1을 처리하지 않은 실험군, (4) EPRS를 과발현 후, TGF-beta1을 처리한 실험군 총 네 가지 조건으로 나누어 qRT-PCR 실험하였다. 이때 상기 TGF-beta1군은 10ng/ml의 농도로 12시간 처리되었다.
그 결과 도 6에서 보는 바와 같이, EPRS 과발현에 따라 Snail1의 relative mRNA level이 변하지 않았고, 이를 통해 EPRS가 Snail1의 mRNA를 조절하는 것 이외의 방법으로 상호작용을 할 것이라 예측할 수 있다.
<2-3> EPRS의 Snail1 안정성 증가 효과 확인
Proteasomal inhibitor인 MG132의 처리를 통해, EPRS가 Snail1의 안정성을 증가시키는 지 알아보기 위한 실험으로, HEK293T 세포에 (1) STREP-tag empty vector와 FLAG-tag empty vector, (2) STREP-tag empty vector와 FLAG tag된 Snail1, (3) STREP tag된 WHEP-PRS와 FLAG-tag empty vector, (4) STREP tag된 ERS-WHEP과 FLAG-tag empty vector, (5) STREP tag된 EPRS와 FLAG-tag empty vector, 위의 (3), (4), (5) 조건에 FLAG-tag empty vector 대신 FLAG tag된 Snail1을 co-transfection한 (6), (7), (8), 그리고 위의 (6), (7), (8) 조건에 MG132를 50 μM, 6시간 처리한 (9), (10), (11)까지 총 열 한 개의 실험군으로 실험하였다. 상기 실험군의 세포들은 각각 용해된 후, anti-Flag antibody로 면역블롯되었다.
그 결과, 도 7에서 보는 바와 같이 단독 과발현에 비해 co-transfection에 의해 EPRS와 함께 과발현된 Snail1의 단백질 발현 수준이 보다 증가 하였으며, EPRS 중 WHEP-PRS 조각 부위가 ERS-WHEP 조각 부위에 비해 Snail1의 안정성에 보다 기여를 하는 것으로 관찰된다. 이는 도 8의 유비퀴틴화 분석에 의해, EPRS의 과발현이 Snail의 폴리 유비퀴틴화를 억제하는 효과가 있음을 보여주는 결과와 일치한다.
<2-4> EPRS 과발현을 통한 Snail1 안정성 증가 효과 확인
EPRS의 과발현이 ubiquitination 억제 작용을 통해 Snail1의 안정성을 조절하는지 알아보기 위한 실험으로, A549 세포에 (1) FLAG-tag empty vector와 STREP tag된 Snail1, (2) FLAG tag된 EPRS와 STREP tag된 Snail1 총 두 가지 조건에 HA tag된 Ubiquitin을 co-transfection한 후, 세포를 회수하기 6시간 전에 MG-132를 50μM 처리하고, Streptavidin bead 를 이용하여 IP하였다.
상기 IP한 시료를 HA antibody로 면역 블롯한 결과, 도 8에서 보는 바와 같이 EPRS가 과발현된 실험군에서 ubiquitination이 감소하였다. Snail1의 조절은 ubiquitination에 의한 degradation이 잘 알려져 있는데 (Zhou, Deng et al. 2004), EPRS가 과발현 되면 Snail1의 ubiquitination을 줄여 이를 통해 Snail1의 안정성이 증가하는 것으로 예측할 수 있다.
<실시예 3>
EPRS 및 Snail의 결합 저해에 따른 EMT 진행억제 효과확인
<3-1> EPRS 넉다운(knock down)이 Snail1과 EMT진행에 미치는 영향 확인
감소된 EPRS가 Snail1과 EMT 진행에 어떠한 영향을 주는 지, siRNA를 이용하여 EPRS의 발현을 knock down한 실험으로 확인하였다. 실험군 별로 A549 세포에 si-control 또는 si-EPRS를 처리하고 TGF-beta1을 10ng/ml 1시간, 48시간으로 처리 또는 비처리하여, Snail1을 비롯한 EMT 관련 마커들(p-SMAD3, SMAD2/3, SLUG, EPRS, E-cadherin, N-cadherin)의 변화를 세포질과 핵으로 나누어 면역블롯 방법으로 관찰하였다. HSP90α/β, p84은 각각 세포질, 핵에서의 단백질 발현양을 확인하는 loading control 로 사용하였다.
그 결과 도 9의 A 및 B에서 보는 바와 같이 EPRS knock down 시 Snail1 및 p-SMAD3이 감소되었으며, E-cadherin은 증가하였다. 이는 EMT 진행이 저해되었음을 나타낸다.
<3-2> EPRS 넉다운(knock down)이 세포 이주(migration)에 미치는 영향 확인
감소된 EPRS가 세포의 migration에 어떠한 영향을 주는 지 알아보기 위한 실험으로, A549 세포에 (1) si-control 처리 후, TGF-beta1을 처리하지 않은 조건, (2) si-control 처리 후, TGF-beta1을 처리한 조건, (3) si-EPRS 처리 후, TGF-beta1을 처리하지 않은 조건, (4) si-EPRS 처리 후, TGF-beta1을 처리한 조건 총 네 가지 실험군에서 세포의 이동성을 관찰하였다. 이때 상기 TGF-beta1군은 10ng/ml의 농도로 12 시간 처리되었다.
그 결과 도 10 및 도 11에서 보는 바와 같이, EPRS knock down 시 세포의 이동성이 감소하였다. 이때, TGF-beta1 처리군 및 비처리군 모두에서 세포의 이동성이 감소하였다.
<3-3> EPRS 넉다운(knock down)이 세포 침투(invasion)에 미치는 영향 확인
감소된 EPRS가 세포의 invasion에 어떠한 영향을 주는 지 알아보기 위한 실험으로, A549 세포에 (1) si-control 처리 후, TGF-beta1을 처리하지 않은 조건, (2) si-control 처리 후, TGF-beta1을 처리한 조건, (3) si-EPRS 처리 후, TGF-beta1을 처리하지 않은 조건, (4) si-EPRS 처리 후, TGF-beta1을 처리한 조건 총 네 가지 실험군에서 세포의 이동성을 관찰하였다. 이때 상기 TGF-beta1군은 10ng/ml의 농도로 12 시간 처리되었다.
그 결과 도 12 및 도 13에서 보는 바와 같이, EPRS knock down 시 상기 실시예 <3-2>에서 확인한 세포의 이동성뿐만 아니라 세포의 침투성 또한 감소하였다. 이때, TGF-beta1 처리군 및 비처리군 모두에서 세포의 침투성이 감소하였다.
세포의 이동성 및 침투성 증가는 EMT 진행 세포(특히 암세포 등)에서의 주요 특징으로서, 상기와 같은 실험결과들을 통하여 EPRS와 Snail 1의 상호작용(결합)을 억제하면 EMT 진행을 억제할 수 있음을 확인하였고 이는 EMT와 관계된 것으로 알려진 암(전이), 섬유증 등의 질환의 치료제 개발에 있어 주요 타겟을 시사한다.
이상 살펴본 바와 같이, 본 발명은 EPRS의 신규한 용도에 관한 것으로, EMT 억제제를 스크리닝하는 방법을 제공한다. 본 발명인의 방법은 암을 포함하여 다양한 EMT 관련 질환의 새로운 치료제의 개발에 유용하게 이용될 수 있어 산업상 이용가능성이 크다.
<110> Medicinal Bioconvergence Research Center <120> Method for screening EMT inhibitor <130> NP14-0067 <160> 203 <170> KopatentIn 2.0 <210> 1 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> Wild type EPRS (whole amino acid sequence)_[Homo sapiens] GenBank: AAI26276.1 accession BC126275.1 <400> 1 Met Ala Thr Leu Ser Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Thr Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Asp Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Val Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Val Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro 725 730 735 Thr Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser 805 810 815 Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ser Pro Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu 885 890 895 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val 915 920 925 Asp Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn 980 985 990 Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 His Asp Ile Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile 1045 1050 1055 Trp Glu Ala Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Ser Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Ile Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 Gln Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 2 <211> 1000 <212> PRT <213> Artificial Sequence <220> <223> ERS-WHEP domain _amino acid sequence <400> 2 Met Ala Thr Leu Ser Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Thr Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Asp Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Val Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Val Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro 725 730 735 Thr Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser 805 810 815 Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ser Pro Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu 885 890 895 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val 915 920 925 Asp Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn 980 985 990 Gln Gly Gly Gly Leu Ser Ser Ser 995 1000 <210> 3 <211> 720 <212> PRT <213> Artificial Sequence <220> <223> ERS domain _amino acid sequence <400> 3 Met Ala Thr Leu Ser Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Thr Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Asp Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Val Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Val Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 <210> 4 <211> 792 <212> PRT <213> Artificial Sequence <220> <223> WHEP-PRS domain _amino acid sequence <400> 4 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro 1 5 10 15 Thr Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val 20 25 30 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 35 40 45 Ala Lys Lys Ala Pro Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu 50 55 60 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 65 70 75 80 Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser 85 90 95 Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 100 105 110 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ser Pro Lys Ala 115 120 125 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 130 135 140 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 145 150 155 160 Gln Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu 165 170 175 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 180 185 190 Glu Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val 195 200 205 Asp Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 210 215 220 Leu Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 225 230 235 240 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 245 250 255 Lys Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn 260 265 270 Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro 275 280 285 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 290 295 300 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 305 310 315 320 His Asp Ile Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile 325 330 335 Trp Glu Ala Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 340 345 350 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Ser Ala Leu Glu 355 360 365 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 370 375 380 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 385 390 395 400 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 405 410 415 His Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg 420 425 430 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 435 440 445 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu 450 455 460 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 465 470 475 480 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 485 490 495 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 500 505 510 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 515 520 525 Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Ile Pro Gly Glu 530 535 540 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 545 550 555 560 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 565 570 575 Pro Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr 580 585 590 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn 595 600 605 Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp 610 615 620 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 625 630 635 640 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 645 650 655 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 660 665 670 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 675 680 685 Gln Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 690 695 700 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly 705 710 715 720 Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 725 730 735 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 740 745 750 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 755 760 765 Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr 770 775 780 Tyr Thr Leu Phe Gly Arg Ser Tyr 785 790 <210> 5 <211> 512 <212> PRT <213> Artificial Sequence <220> <223> PRS domain _amino acid sequence <400> 5 Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu 1 5 10 15 Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile 20 25 30 Thr Lys Ser Glu Met Ile Glu Tyr His Asp Ile Ser Gly Cys Tyr Ile 35 40 45 Leu Arg Pro Trp Ala Tyr Ala Ile Trp Glu Ala Ile Lys Asp Phe Phe 50 55 60 Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met 65 70 75 80 Phe Val Ser Gln Ser Ala Leu Glu Lys Glu Lys Thr His Val Ala Asp 85 90 95 Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu 100 105 110 Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro 115 120 125 Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Lys Leu 130 135 140 Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro 145 150 155 160 Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe 165 170 175 Ala Thr Met Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu 180 185 190 Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly 195 200 205 Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr 210 215 220 Ile Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Gly Thr 225 230 235 240 Ser His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Val Phe 245 250 255 Glu Asp Pro Lys Ile Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser 260 265 270 Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly 275 280 285 Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val 290 295 300 Val Ile Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Lys 305 310 315 320 Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser 325 330 335 Val Asn Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly 340 345 350 Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu 355 360 365 Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg 370 375 380 Asp Thr Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys 385 390 395 400 Leu Gln Ala Ile Leu Glu Asp Ile Gln Val Thr Leu Phe Thr Arg Ala 405 410 415 Ser Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp 420 425 430 Phe Gln Lys Ile Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys 435 440 445 Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp 450 455 460 Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys 465 470 475 480 Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Val 485 490 495 Cys Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 500 505 510 <210> 6 <211> 1232 <212> PRT <213> Artificial Sequence <220> <223> ERS-PRS domain _amino acid sequence <400> 6 Met Ala Thr Leu Ser Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Thr Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Asp Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Val Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Val Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu 725 730 735 Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile 740 745 750 Thr Lys Ser Glu Met Ile Glu Tyr His Asp Ile Ser Gly Cys Tyr Ile 755 760 765 Leu Arg Pro Trp Ala Tyr Ala Ile Trp Glu Ala Ile Lys Asp Phe Phe 770 775 780 Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met 785 790 795 800 Phe Val Ser Gln Ser Ala Leu Glu Lys Glu Lys Thr His Val Ala Asp 805 810 815 Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu 820 825 830 Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro 835 840 845 Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Lys Leu 850 855 860 Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro 865 870 875 880 Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe 885 890 895 Ala Thr Met Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu 900 905 910 Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly 915 920 925 Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr 930 935 940 Ile Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Gly Thr 945 950 955 960 Ser His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Val Phe 965 970 975 Glu Asp Pro Lys Ile Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser 980 985 990 Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly 995 1000 1005 Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val 1010 1015 1020 Val Ile Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Lys 1025 1030 1035 1040 Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser 1045 1050 1055 Val Asn Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly 1060 1065 1070 Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu 1075 1080 1085 Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg 1090 1095 1100 Asp Thr Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys 1105 1110 1115 1120 Leu Gln Ala Ile Leu Glu Asp Ile Gln Val Thr Leu Phe Thr Arg Ala 1125 1130 1135 Ser Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp 1140 1145 1150 Phe Gln Lys Ile Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys 1155 1160 1165 Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp 1170 1175 1180 Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys 1185 1190 1195 1200 Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Val 1205 1210 1215 Cys Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1220 1225 1230 <210> 7 <211> 280 <212> PRT <213> Artificial Sequence <220> <223> WHEP domain _amino acid sequence <400> 7 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro 1 5 10 15 Thr Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val 20 25 30 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 35 40 45 Ala Lys Lys Ala Pro Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu 50 55 60 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 65 70 75 80 Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser 85 90 95 Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 100 105 110 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ser Pro Lys Ala 115 120 125 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 130 135 140 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 145 150 155 160 Gln Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu 165 170 175 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 180 185 190 Glu Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val 195 200 205 Asp Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 210 215 220 Leu Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 225 230 235 240 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 245 250 255 Lys Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn 260 265 270 Gln Gly Gly Gly Leu Ser Ser Ser 275 280 <210> 8 <211> 4701 <212> DNA <213> Artificial Sequence <220> <223> Wild type EPRS (whole nucleotide sequence)_Homo sapiens glutamyl-prolyl-tRNA synthetase, mRNA (cDNA clone MGC:161553 IMAGE:8991991), complete CDs GenBank: BC126275.1 <400> 8 ccaggcgtac tgacaggtgg ccaggcgtac tgacaggtgg ccaggcgtac tgacaggtgg 60 ccaggcgtac tgacaggtgg ccaggcgtac tgacaggtgg ccaggcgtac tgacaggtgg 120 accagcggac tggtggagat ggcgacgctc tctctgaccg tgaattcagg agaccctccg 180 ctaggagctt tgctggcagt agaacacgtg aaagacgatg tcagcatttc cgttgaagaa 240 gggaaagaga atattcttca tgtttctgaa aatgtgatat tcacagatgt gaattctata 300 cttcgctact tggctagagt tgcaactaca gctgggttat atggctctaa tctgatggaa 360 catactgaga ttgatcactg gttggagttc agtgctacaa aattatcttc atgtgattcc 420 tttacttcta caattaatga actcaatcat tgcctgtctc tgagaacata cttagttgga 480 aactccttga gtttagcaga tttatgtgtt tgggccaccc taaaaggaaa tgctgcctgg 540 caagaacagt tgaaacagaa gaaagctcca gttcatgtaa aacgttggtt tggctttctt 600 gaagcccagc aggccttcca gtcagtaggt accaagtggg atgtttcaac aaccaaagct 660 cgagtggcac ctgagaaaaa gcaagatgtt gggaaatttg ttgagcttcc aggtgcggag 720 atgggaaagg ttaccgtcag atttcctcca gaggccagtg gttacttaca cattgggcat 780 gcaaaagctg ctcttctgaa ccagcactac caggttaact ttaaagggaa actgatcatg 840 agatttgatg acacaaatcc tgaaaaagaa aaggaagatt ttgagaaggt tatcttggaa 900 gatgttgcaa tgttgcatat caaaccagat caatttactt atacttcgga tcattttgaa 960 actataatga agtatgcaga gaagctaatt caagaaggga aggcttatgt ggatgatact 1020 cctgctgaac agatgaaagc agaacgtgag cagaggatag actctaaaca tagaaaaaac 1080 cctattgaga agaatctaca aatgtgggaa gaaatgaaaa aagggagcca gtttggtcag 1140 tcctgttgtt tgcgagcaaa aattgacatg agtagtaaca atggatgcat gagagatcca 1200 accctttatc gctgcaaaat tcaaccacat ccaagaactg gaaataaata caatgtttat 1260 ccaacatatg attttgcctg ccccatagtt gacagcatcg aaggtgttac acatgccctg 1320 agaacaacag aataccatga cagagatgag cagttttact ggattattga agctttaggc 1380 ataagaaaac catatatttg ggaatatagt cggctaaatc tcaacaacac agtgctatcc 1440 aaaagaaaac tcacatggtt tgtcaatgaa ggactagtag atggatggga tgacccaaga 1500 tttcctacgg ttcgtggtgt actgagaaga gggatgacag ttgaaggact gaaacagttt 1560 attgctgctc agggctcctc acgttcagtc gtgaacatgg agtgggacaa aatctgggcg 1620 tttaacaaaa aggttattga cccagtggct ccacgatatg ttgcattact gaagaaagaa 1680 gtgatcccag tgaatgtacc tgaagctcag gaggagatga aagaagtagc caaacaccca 1740 aagaatcctg aggttggctt gaagcctgtg tggtatagtc ccaaagtttt cattgaaggt 1800 gctgatgcag agactttttc ggagggtgag atggttacat ttataaattg gggcaacctc 1860 aacattacaa aaatacacaa aaatgcagat ggaaaaatca tatctcttga tgcaaagttg 1920 aatttggaaa acaaagacta caagaaaacc actaaggtca cttggcttgc agagactaca 1980 catgctcttc ctattccagt aatctgtgtc acttatgagc acttgatcac aaagccagtg 2040 ctaggaaaag acgaggactt taagcagtat gtcaacaaga acagtaagca tgaagagcta 2100 atgctagggg atccctgcct taaggatttg aaaaaaggag atattataca actccagaga 2160 agaggattct tcatatgtga tcaaccttat gaacctgtta gcccatatag ttgcaaggaa 2220 gccccgtgtg ttttgatata cattcctgat gggcacacaa aggaaatgcc aacatcaggg 2280 tcaaaggaaa agaccaaagt agaagccaca aaaaatgaga cctctgctcc ttttaaggaa 2340 agaccaacac cttctctgaa taataattgt actacatctg aggattcctt ggtcctttac 2400 aatagagtgg ctgttcaagg agatgtggtt cgtgaattaa aagccaagaa agcaccaaag 2460 gaagatgtag atgcagctgt aaaacagctt ttgtctttga aagctgaata taaggagaaa 2520 actggccagg aatataaacc tggaaaccct cctgctgaaa taggacagaa tatttcttct 2580 aattcctcag caagtattct ggaaagtaaa tctctgtatg atgaagttgc tgcacaaggg 2640 gaggtggttc gtaagctaaa agctgaaaaa tcccctaagg ctaaaataaa tgaagctgta 2700 gaatgcttac tgtccctgaa ggctcagtat aaagaaaaaa ctgggaagga gtacatacct 2760 ggtcagcccc cattatctca aagttcggat tcaagcccaa ccagaaattc tgaacctgct 2820 ggtttagaaa caccagaagc gaaagtactt tttgacaaag tagcttctca aggggaagta 2880 gttcggaaac ttaaaactga aaaagcccct aaggatcaag tagatatagc tgttcaagaa 2940 ctccttcagc taaaggcaca gtacaagtct ttgataggag tagagtataa gcctgtgtcg 3000 gccactggag ctgaggacaa agataagaag aagaaagaaa aagaaaataa atctgaaaag 3060 cagaataagc ctcagaaaca aaatgatggc caaaggaaag acccttctaa aaaccaagga 3120 ggtgggctct catcaagtgg agcaggagaa gggcaggggc ctaagaaaca gaccaggttg 3180 ggtcttgagg caaaaaaaga agaaaatctt gctgattggt attctcaggt catcacaaag 3240 tcagaaatga ttgaatacca tgacataagt ggctgttata ttcttcgtcc ctgggcctat 3300 gccatttggg aagccatcaa ggactttttt gatgctgaga tcaagaaact tggtgttgaa 3360 aactgctact tccccatgtt tgtgtctcaa agtgcattag agaaagagaa gactcatgtt 3420 gctgactttg ccccagaggt tgcttgggtt acaagatctg gcaaaaccga gctggcagaa 3480 ccaattgcca ttcgtcctac tagtgaaaca gtaatgtatc ctgcatatgc aaaatgggta 3540 cagtcacaca gagacctgcc catcaagctc aatcagtggt gcaatgtggt gcgttgggaa 3600 ttcaagcatc ctcagccttt cctacgtact cgtgaatttc tttggcagga agggcacagt 3660 gcttttgcta ccatggaaga ggcagcggaa gaggtcttgc agatacttga cttatatgct 3720 caggtatatg aagaactcct ggcaattcct gttgttaaag gaagaaagac ggaaaaggaa 3780 aaatttgcag gaggagacta tacaactaca atagaagcat ttatatctgc tagtggaaga 3840 gctatccagg gaggaacatc acatcattta gggcagaatt tttccaaaat gtttgaaatc 3900 gtttttgaag atccaaagat accaggagag aagcaatttg cctatcaaaa ctcctggggc 3960 ctgacaactc gaactattgg tgttatgacc atggttcatg gggacaacat gggtttagta 4020 ttaccacccc gtgtagcatg tgttcaggtg gtgattattc cttgtggcat taccaatgca 4080 ctttctgaag aagacaaaga agcgctgatt gcaaaatgca atgattatcg aaggcgatta 4140 ctcagtgtta acatccgcgt tagagctgat ttacgagata attattctcc aggttggaaa 4200 ttcaatcact gggagctcaa gggagttccc attagacttg aagttgggcc acgtgatatg 4260 aagagctgtc agtttgtagc cgtcagacga gatactggag aaaagctgac agttgctgaa 4320 aatgaggcag agactaaact tcaagctatt ttggaagaca tccaggtcac ccttttcaca 4380 agggcttctg aagaccttaa gactcatatg gttgtggcta atacaatgga agactttcag 4440 aagatactag attctggaaa gattgttcag attccattct gtggggaaat tgactgtgag 4500 gactggatca aaaagaccac tgccagggat caagatcttg aacctggtgc tccatccatg 4560 ggagctaaaa gcctttgcat ccccttcaaa ccactctgtg aactgcagcc tggagccaaa 4620 tgtgtctgtg gcaagaaccc tgccaagtac tacaccttat ttggtcgcag ctactgaggg 4680 atgaacgaaa gccccctctt c 4701 <210> 9 <211> 3000 <212> DNA <213> Artificial Sequence <220> <223> ERS-WHEP domain _nucleotide sequence <400> 9 atggcgacgc tctctctgac cgtgaattca ggagaccctc cgctaggagc tttgctggca 60 gtagaacacg tgaaagacga tgtcagcatt tccgttgaag aagggaaaga gaatattctt 120 catgtttctg aaaatgtgat attcacagat gtgaattcta tacttcgcta cttggctaga 180 gttgcaacta cagctgggtt atatggctct aatctgatgg aacatactga gattgatcac 240 tggttggagt tcagtgctac aaaattatct tcatgtgatt cctttacttc tacaattaat 300 gaactcaatc attgcctgtc tctgagaaca tacttagttg gaaactcctt gagtttagca 360 gatttatgtg tttgggccac cctaaaagga aatgctgcct ggcaagaaca gttgaaacag 420 aagaaagctc cagttcatgt aaaacgttgg tttggctttc ttgaagccca gcaggccttc 480 cagtcagtag gtaccaagtg ggatgtttca acaaccaaag ctcgagtggc acctgagaaa 540 aagcaagatg ttgggaaatt tgttgagctt ccaggtgcgg agatgggaaa ggttaccgtc 600 agatttcctc cagaggccag tggttactta cacattgggc atgcaaaagc tgctcttctg 660 aaccagcact accaggttaa ctttaaaggg aaactgatca tgagatttga tgacacaaat 720 cctgaaaaag aaaaggaaga ttttgagaag gttatcttgg aagatgttgc aatgttgcat 780 atcaaaccag atcaatttac ttatacttcg gatcattttg aaactataat gaagtatgca 840 gagaagctaa ttcaagaagg gaaggcttat gtggatgata ctcctgctga acagatgaaa 900 gcagaacgtg agcagaggat agactctaaa catagaaaaa accctattga gaagaatcta 960 caaatgtggg aagaaatgaa aaaagggagc cagtttggtc agtcctgttg tttgcgagca 1020 aaaattgaca tgagtagtaa caatggatgc atgagagatc caacccttta tcgctgcaaa 1080 attcaaccac atccaagaac tggaaataaa tacaatgttt atccaacata tgattttgcc 1140 tgccccatag ttgacagcat cgaaggtgtt acacatgccc tgagaacaac agaataccat 1200 gacagagatg agcagtttta ctggattatt gaagctttag gcataagaaa accatatatt 1260 tgggaatata gtcggctaaa tctcaacaac acagtgctat ccaaaagaaa actcacatgg 1320 tttgtcaatg aaggactagt agatggatgg gatgacccaa gatttcctac ggttcgtggt 1380 gtactgagaa gagggatgac agttgaagga ctgaaacagt ttattgctgc tcagggctcc 1440 tcacgttcag tcgtgaacat ggagtgggac aaaatctggg cgtttaacaa aaaggttatt 1500 gacccagtgg ctccacgata tgttgcatta ctgaagaaag aagtgatccc agtgaatgta 1560 cctgaagctc aggaggagat gaaagaagta gccaaacacc caaagaatcc tgaggttggc 1620 ttgaagcctg tgtggtatag tcccaaagtt ttcattgaag gtgctgatgc agagactttt 1680 tcggagggtg agatggttac atttataaat tggggcaacc tcaacattac aaaaatacac 1740 aaaaatgcag atggaaaaat catatctctt gatgcaaagt tgaatttgga aaacaaagac 1800 tacaagaaaa ccactaaggt cacttggctt gcagagacta cacatgctct tcctattcca 1860 gtaatctgtg tcacttatga gcacttgatc acaaagccag tgctaggaaa agacgaggac 1920 tttaagcagt atgtcaacaa gaacagtaag catgaagagc taatgctagg ggatccctgc 1980 cttaaggatt tgaaaaaagg agatattata caactccaga gaagaggatt cttcatatgt 2040 gatcaacctt atgaacctgt tagcccatat agttgcaagg aagccccgtg tgttttgata 2100 tacattcctg atgggcacac aaaggaaatg ccaacatcag ggtcaaagga aaagaccaaa 2160 gtagaagcca caaaaaatga gacctctgct ccttttaagg aaagaccaac accttctctg 2220 aataataatt gtactacatc tgaggattcc ttggtccttt acaatagagt ggctgttcaa 2280 ggagatgtgg ttcgtgaatt aaaagccaag aaagcaccaa aggaagatgt agatgcagct 2340 gtaaaacagc ttttgtcttt gaaagctgaa tataaggaga aaactggcca ggaatataaa 2400 cctggaaacc ctcctgctga aataggacag aatatttctt ctaattcctc agcaagtatt 2460 ctggaaagta aatctctgta tgatgaagtt gctgcacaag gggaggtggt tcgtaagcta 2520 aaagctgaaa aatcccctaa ggctaaaata aatgaagctg tagaatgctt actgtccctg 2580 aaggctcagt ataaagaaaa aactgggaag gagtacatac ctggtcagcc cccattatct 2640 caaagttcgg attcaagccc aaccagaaat tctgaacctg ctggtttaga aacaccagaa 2700 gcgaaagtac tttttgacaa agtagcttct caaggggaag tagttcggaa acttaaaact 2760 gaaaaagccc ctaaggatca agtagatata gctgttcaag aactccttca gctaaaggca 2820 cagtacaagt ctttgatagg agtagagtat aagcctgtgt cggccactgg agctgaggac 2880 aaagataaga agaagaaaga aaaagaaaat aaatctgaaa agcagaataa gcctcagaaa 2940 caaaatgatg gccaaaggaa agacccttct aaaaaccaag gaggtgggct ctcatcaagt 3000 3000 <210> 10 <211> 2160 <212> DNA <213> Artificial Sequence <220> <223> ERS domain _nucleotide sequence <400> 10 atggcgacgc tctctctgac cgtgaattca ggagaccctc cgctaggagc tttgctggca 60 gtagaacacg tgaaagacga tgtcagcatt tccgttgaag aagggaaaga gaatattctt 120 catgtttctg aaaatgtgat attcacagat gtgaattcta tacttcgcta cttggctaga 180 gttgcaacta cagctgggtt atatggctct aatctgatgg aacatactga gattgatcac 240 tggttggagt tcagtgctac aaaattatct tcatgtgatt cctttacttc tacaattaat 300 gaactcaatc attgcctgtc tctgagaaca tacttagttg gaaactcctt gagtttagca 360 gatttatgtg tttgggccac cctaaaagga aatgctgcct ggcaagaaca gttgaaacag 420 aagaaagctc cagttcatgt aaaacgttgg tttggctttc ttgaagccca gcaggccttc 480 cagtcagtag gtaccaagtg ggatgtttca acaaccaaag ctcgagtggc acctgagaaa 540 aagcaagatg ttgggaaatt tgttgagctt ccaggtgcgg agatgggaaa ggttaccgtc 600 agatttcctc cagaggccag tggttactta cacattgggc atgcaaaagc tgctcttctg 660 aaccagcact accaggttaa ctttaaaggg aaactgatca tgagatttga tgacacaaat 720 cctgaaaaag aaaaggaaga ttttgagaag gttatcttgg aagatgttgc aatgttgcat 780 atcaaaccag atcaatttac ttatacttcg gatcattttg aaactataat gaagtatgca 840 gagaagctaa ttcaagaagg gaaggcttat gtggatgata ctcctgctga acagatgaaa 900 gcagaacgtg agcagaggat agactctaaa catagaaaaa accctattga gaagaatcta 960 caaatgtggg aagaaatgaa aaaagggagc cagtttggtc agtcctgttg tttgcgagca 1020 aaaattgaca tgagtagtaa caatggatgc atgagagatc caacccttta tcgctgcaaa 1080 attcaaccac atccaagaac tggaaataaa tacaatgttt atccaacata tgattttgcc 1140 tgccccatag ttgacagcat cgaaggtgtt acacatgccc tgagaacaac agaataccat 1200 gacagagatg agcagtttta ctggattatt gaagctttag gcataagaaa accatatatt 1260 tgggaatata gtcggctaaa tctcaacaac acagtgctat ccaaaagaaa actcacatgg 1320 tttgtcaatg aaggactagt agatggatgg gatgacccaa gatttcctac ggttcgtggt 1380 gtactgagaa gagggatgac agttgaagga ctgaaacagt ttattgctgc tcagggctcc 1440 tcacgttcag tcgtgaacat ggagtgggac aaaatctggg cgtttaacaa aaaggttatt 1500 gacccagtgg ctccacgata tgttgcatta ctgaagaaag aagtgatccc agtgaatgta 1560 cctgaagctc aggaggagat gaaagaagta gccaaacacc caaagaatcc tgaggttggc 1620 ttgaagcctg tgtggtatag tcccaaagtt ttcattgaag gtgctgatgc agagactttt 1680 tcggagggtg agatggttac atttataaat tggggcaacc tcaacattac aaaaatacac 1740 aaaaatgcag atggaaaaat catatctctt gatgcaaagt tgaatttgga aaacaaagac 1800 tacaagaaaa ccactaaggt cacttggctt gcagagacta cacatgctct tcctattcca 1860 gtaatctgtg tcacttatga gcacttgatc acaaagccag tgctaggaaa agacgaggac 1920 tttaagcagt atgtcaacaa gaacagtaag catgaagagc taatgctagg ggatccctgc 1980 cttaaggatt tgaaaaaagg agatattata caactccaga gaagaggatt cttcatatgt 2040 gatcaacctt atgaacctgt tagcccatat agttgcaagg aagccccgtg tgttttgata 2100 tacattcctg atgggcacac aaaggaaatg ccaacatcag ggtcaaagga aaagaccaaa 2160 2160 <210> 11 <211> 2379 <212> DNA <213> Artificial Sequence <220> <223> WHEP-PRS domain _nucleotide sequence <400> 11 gtagaagcca caaaaaatga gacctctgct ccttttaagg aaagaccaac accttctctg 60 aataataatt gtactacatc tgaggattcc ttggtccttt acaatagagt ggctgttcaa 120 ggagatgtgg ttcgtgaatt aaaagccaag aaagcaccaa aggaagatgt agatgcagct 180 gtaaaacagc ttttgtcttt gaaagctgaa tataaggaga aaactggcca ggaatataaa 240 cctggaaacc ctcctgctga aataggacag aatatttctt ctaattcctc agcaagtatt 300 ctggaaagta aatctctgta tgatgaagtt gctgcacaag gggaggtggt tcgtaagcta 360 aaagctgaaa aatcccctaa ggctaaaata aatgaagctg tagaatgctt actgtccctg 420 aaggctcagt ataaagaaaa aactgggaag gagtacatac ctggtcagcc cccattatct 480 caaagttcgg attcaagccc aaccagaaat tctgaacctg ctggtttaga aacaccagaa 540 gcgaaagtac tttttgacaa agtagcttct caaggggaag tagttcggaa acttaaaact 600 gaaaaagccc ctaaggatca agtagatata gctgttcaag aactccttca gctaaaggca 660 cagtacaagt ctttgatagg agtagagtat aagcctgtgt cggccactgg agctgaggac 720 aaagataaga agaagaaaga aaaagaaaat aaatctgaaa agcagaataa gcctcagaaa 780 caaaatgatg gccaaaggaa agacccttct aaaaaccaag gaggtgggct ctcatcaagt 840 ggagcaggag aagggcaggg gcctaagaaa cagaccaggt tgggtcttga ggcaaaaaaa 900 gaagaaaatc ttgctgattg gtattctcag gtcatcacaa agtcagaaat gattgaatac 960 catgacataa gtggctgtta tattcttcgt ccctgggcct atgccatttg ggaagccatc 1020 aaggactttt ttgatgctga gatcaagaaa cttggtgttg aaaactgcta cttccccatg 1080 tttgtgtctc aaagtgcatt agagaaagag aagactcatg ttgctgactt tgccccagag 1140 gttgcttggg ttacaagatc tggcaaaacc gagctggcag aaccaattgc cattcgtcct 1200 actagtgaaa cagtaatgta tcctgcatat gcaaaatggg tacagtcaca cagagacctg 1260 cccatcaagc tcaatcagtg gtgcaatgtg gtgcgttggg aattcaagca tcctcagcct 1320 ttcctacgta ctcgtgaatt tctttggcag gaagggcaca gtgcttttgc taccatggaa 1380 gaggcagcgg aagaggtctt gcagatactt gacttatatg ctcaggtata tgaagaactc 1440 ctggcaattc ctgttgttaa aggaagaaag acggaaaagg aaaaatttgc aggaggagac 1500 tatacaacta caatagaagc atttatatct gctagtggaa gagctatcca gggaggaaca 1560 tcacatcatt tagggcagaa tttttccaaa atgtttgaaa tcgtttttga agatccaaag 1620 ataccaggag agaagcaatt tgcctatcaa aactcctggg gcctgacaac tcgaactatt 1680 ggtgttatga ccatggttca tggggacaac atgggtttag tattaccacc ccgtgtagca 1740 tgtgttcagg tggtgattat tccttgtggc attaccaatg cactttctga agaagacaaa 1800 gaagcgctga ttgcaaaatg caatgattat cgaaggcgat tactcagtgt taacatccgc 1860 gttagagctg atttacgaga taattattct ccaggttgga aattcaatca ctgggagctc 1920 aagggagttc ccattagact tgaagttggg ccacgtgata tgaagagctg tcagtttgta 1980 gccgtcagac gagatactgg agaaaagctg acagttgctg aaaatgaggc agagactaaa 2040 cttcaagcta ttttggaaga catccaggtc acccttttca caagggcttc tgaagacctt 2100 aagactcata tggttgtggc taatacaatg gaagactttc agaagatact agattctgga 2160 aagattgttc agattccatt ctgtggggaa attgactgtg aggactggat caaaaagacc 2220 actgccaggg atcaagatct tgaacctggt gctccatcca tgggagctaa aagcctttgc 2280 atccccttca aaccactctg tgaactgcag cctggagcca aatgtgtctg tggcaagaac 2340 cctgccaagt actacacctt atttggtcgc agctactga 2379 <210> 12 <211> 1539 <212> DNA <213> Artificial Sequence <220> <223> PRS domain _nucleotide sequence <400> 12 ggagcaggag aagggcaggg gcctaagaaa cagaccaggt tgggtcttga ggcaaaaaaa 60 gaagaaaatc ttgctgattg gtattctcag gtcatcacaa agtcagaaat gattgaatac 120 catgacataa gtggctgtta tattcttcgt ccctgggcct atgccatttg ggaagccatc 180 aaggactttt ttgatgctga gatcaagaaa cttggtgttg aaaactgcta cttccccatg 240 tttgtgtctc aaagtgcatt agagaaagag aagactcatg ttgctgactt tgccccagag 300 gttgcttggg ttacaagatc tggcaaaacc gagctggcag aaccaattgc cattcgtcct 360 actagtgaaa cagtaatgta tcctgcatat gcaaaatggg tacagtcaca cagagacctg 420 cccatcaagc tcaatcagtg gtgcaatgtg gtgcgttggg aattcaagca tcctcagcct 480 ttcctacgta ctcgtgaatt tctttggcag gaagggcaca gtgcttttgc taccatggaa 540 gaggcagcgg aagaggtctt gcagatactt gacttatatg ctcaggtata tgaagaactc 600 ctggcaattc ctgttgttaa aggaagaaag acggaaaagg aaaaatttgc aggaggagac 660 tatacaacta caatagaagc atttatatct gctagtggaa gagctatcca gggaggaaca 720 tcacatcatt tagggcagaa tttttccaaa atgtttgaaa tcgtttttga agatccaaag 780 ataccaggag agaagcaatt tgcctatcaa aactcctggg gcctgacaac tcgaactatt 840 ggtgttatga ccatggttca tggggacaac atgggtttag tattaccacc ccgtgtagca 900 tgtgttcagg tggtgattat tccttgtggc attaccaatg cactttctga agaagacaaa 960 gaagcgctga ttgcaaaatg caatgattat cgaaggcgat tactcagtgt taacatccgc 1020 gttagagctg atttacgaga taattattct ccaggttgga aattcaatca ctgggagctc 1080 aagggagttc ccattagact tgaagttggg ccacgtgata tgaagagctg tcagtttgta 1140 gccgtcagac gagatactgg agaaaagctg acagttgctg aaaatgaggc agagactaaa 1200 cttcaagcta ttttggaaga catccaggtc acccttttca caagggcttc tgaagacctt 1260 aagactcata tggttgtggc taatacaatg gaagactttc agaagatact agattctgga 1320 aagattgttc agattccatt ctgtggggaa attgactgtg aggactggat caaaaagacc 1380 actgccaggg atcaagatct tgaacctggt gctccatcca tgggagctaa aagcctttgc 1440 atccccttca aaccactctg tgaactgcag cctggagcca aatgtgtctg tggcaagaac 1500 cctgccaagt actacacctt atttggtcgc agctactga 1539 <210> 13 <211> 3699 <212> DNA <213> Artificial Sequence <220> <223> ERS-PRS domain _nucleotide sequence <400> 13 atggcgacgc tctctctgac cgtgaattca ggagaccctc cgctaggagc tttgctggca 60 gtagaacacg tgaaagacga tgtcagcatt tccgttgaag aagggaaaga gaatattctt 120 catgtttctg aaaatgtgat attcacagat gtgaattcta tacttcgcta cttggctaga 180 gttgcaacta cagctgggtt atatggctct aatctgatgg aacatactga gattgatcac 240 tggttggagt tcagtgctac aaaattatct tcatgtgatt cctttacttc tacaattaat 300 gaactcaatc attgcctgtc tctgagaaca tacttagttg gaaactcctt gagtttagca 360 gatttatgtg tttgggccac cctaaaagga aatgctgcct ggcaagaaca gttgaaacag 420 aagaaagctc cagttcatgt aaaacgttgg tttggctttc ttgaagccca gcaggccttc 480 cagtcagtag gtaccaagtg ggatgtttca acaaccaaag ctcgagtggc acctgagaaa 540 aagcaagatg ttgggaaatt tgttgagctt ccaggtgcgg agatgggaaa ggttaccgtc 600 agatttcctc cagaggccag tggttactta cacattgggc atgcaaaagc tgctcttctg 660 aaccagcact accaggttaa ctttaaaggg aaactgatca tgagatttga tgacacaaat 720 cctgaaaaag aaaaggaaga ttttgagaag gttatcttgg aagatgttgc aatgttgcat 780 atcaaaccag atcaatttac ttatacttcg gatcattttg aaactataat gaagtatgca 840 gagaagctaa ttcaagaagg gaaggcttat gtggatgata ctcctgctga acagatgaaa 900 gcagaacgtg agcagaggat agactctaaa catagaaaaa accctattga gaagaatcta 960 caaatgtggg aagaaatgaa aaaagggagc cagtttggtc agtcctgttg tttgcgagca 1020 aaaattgaca tgagtagtaa caatggatgc atgagagatc caacccttta tcgctgcaaa 1080 attcaaccac atccaagaac tggaaataaa tacaatgttt atccaacata tgattttgcc 1140 tgccccatag ttgacagcat cgaaggtgtt acacatgccc tgagaacaac agaataccat 1200 gacagagatg agcagtttta ctggattatt gaagctttag gcataagaaa accatatatt 1260 tgggaatata gtcggctaaa tctcaacaac acagtgctat ccaaaagaaa actcacatgg 1320 tttgtcaatg aaggactagt agatggatgg gatgacccaa gatttcctac ggttcgtggt 1380 gtactgagaa gagggatgac agttgaagga ctgaaacagt ttattgctgc tcagggctcc 1440 tcacgttcag tcgtgaacat ggagtgggac aaaatctggg cgtttaacaa aaaggttatt 1500 gacccagtgg ctccacgata tgttgcatta ctgaagaaag aagtgatccc agtgaatgta 1560 cctgaagctc aggaggagat gaaagaagta gccaaacacc caaagaatcc tgaggttggc 1620 ttgaagcctg tgtggtatag tcccaaagtt ttcattgaag gtgctgatgc agagactttt 1680 tcggagggtg agatggttac atttataaat tggggcaacc tcaacattac aaaaatacac 1740 aaaaatgcag atggaaaaat catatctctt gatgcaaagt tgaatttgga aaacaaagac 1800 tacaagaaaa ccactaaggt cacttggctt gcagagacta cacatgctct tcctattcca 1860 gtaatctgtg tcacttatga gcacttgatc acaaagccag tgctaggaaa agacgaggac 1920 tttaagcagt atgtcaacaa gaacagtaag catgaagagc taatgctagg ggatccctgc 1980 cttaaggatt tgaaaaaagg agatattata caactccaga gaagaggatt cttcatatgt 2040 gatcaacctt atgaacctgt tagcccatat agttgcaagg aagccccgtg tgttttgata 2100 tacattcctg atgggcacac aaaggaaatg ccaacatcag ggtcaaagga aaagaccaaa 2160 ggagcaggag aagggcaggg gcctaagaaa cagaccaggt tgggtcttga ggcaaaaaaa 2220 gaagaaaatc ttgctgattg gtattctcag gtcatcacaa agtcagaaat gattgaatac 2280 catgacataa gtggctgtta tattcttcgt ccctgggcct atgccatttg ggaagccatc 2340 aaggactttt ttgatgctga gatcaagaaa cttggtgttg aaaactgcta cttccccatg 2400 tttgtgtctc aaagtgcatt agagaaagag aagactcatg ttgctgactt tgccccagag 2460 gttgcttggg ttacaagatc tggcaaaacc gagctggcag aaccaattgc cattcgtcct 2520 actagtgaaa cagtaatgta tcctgcatat gcaaaatggg tacagtcaca cagagacctg 2580 cccatcaagc tcaatcagtg gtgcaatgtg gtgcgttggg aattcaagca tcctcagcct 2640 ttcctacgta ctcgtgaatt tctttggcag gaagggcaca gtgcttttgc taccatggaa 2700 gaggcagcgg aagaggtctt gcagatactt gacttatatg ctcaggtata tgaagaactc 2760 ctggcaattc ctgttgttaa aggaagaaag acggaaaagg aaaaatttgc aggaggagac 2820 tatacaacta caatagaagc atttatatct gctagtggaa gagctatcca gggaggaaca 2880 tcacatcatt tagggcagaa tttttccaaa atgtttgaaa tcgtttttga agatccaaag 2940 ataccaggag agaagcaatt tgcctatcaa aactcctggg gcctgacaac tcgaactatt 3000 ggtgttatga ccatggttca tggggacaac atgggtttag tattaccacc ccgtgtagca 3060 tgtgttcagg tggtgattat tccttgtggc attaccaatg cactttctga agaagacaaa 3120 gaagcgctga ttgcaaaatg caatgattat cgaaggcgat tactcagtgt taacatccgc 3180 gttagagctg atttacgaga taattattct ccaggttgga aattcaatca ctgggagctc 3240 aagggagttc ccattagact tgaagttggg ccacgtgata tgaagagctg tcagtttgta 3300 gccgtcagac gagatactgg agaaaagctg acagttgctg aaaatgaggc agagactaaa 3360 cttcaagcta ttttggaaga catccaggtc acccttttca caagggcttc tgaagacctt 3420 aagactcata tggttgtggc taatacaatg gaagactttc agaagatact agattctgga 3480 aagattgttc agattccatt ctgtggggaa attgactgtg aggactggat caaaaagacc 3540 actgccaggg atcaagatct tgaacctggt gctccatcca tgggagctaa aagcctttgc 3600 atccccttca aaccactctg tgaactgcag cctggagcca aatgtgtctg tggcaagaac 3660 cctgccaagt actacacctt atttggtcgc agctactga 3699 <210> 14 <211> 840 <212> DNA <213> Artificial Sequence <220> <223> WHEP domain _nucleotide sequence <400> 14 gtagaagcca caaaaaatga gacctctgct ccttttaagg aaagaccaac accttctctg 60 aataataatt gtactacatc tgaggattcc ttggtccttt acaatagagt ggctgttcaa 120 ggagatgtgg ttcgtgaatt aaaagccaag aaagcaccaa aggaagatgt agatgcagct 180 gtaaaacagc ttttgtcttt gaaagctgaa tataaggaga aaactggcca ggaatataaa 240 cctggaaacc ctcctgctga aataggacag aatatttctt ctaattcctc agcaagtatt 300 ctggaaagta aatctctgta tgatgaagtt gctgcacaag gggaggtggt tcgtaagcta 360 aaagctgaaa aatcccctaa ggctaaaata aatgaagctg tagaatgctt actgtccctg 420 aaggctcagt ataaagaaaa aactgggaag gagtacatac ctggtcagcc cccattatct 480 caaagttcgg attcaagccc aaccagaaat tctgaacctg ctggtttaga aacaccagaa 540 gcgaaagtac tttttgacaa agtagcttct caaggggaag tagttcggaa acttaaaact 600 gaaaaagccc ctaaggatca agtagatata gctgttcaag aactccttca gctaaaggca 660 cagtacaagt ctttgatagg agtagagtat aagcctgtgt cggccactgg agctgaggac 720 aaagataaga agaagaaaga aaaagaaaat aaatctgaaa agcagaataa gcctcagaaa 780 caaaatgatg gccaaaggaa agacccttct aaaaaccaag gaggtgggct ctcatcaagt 840 840 <210> 15 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Wild type Snail1(whole amino acid sequence)_zinc finger protein SNAI1 [Homo sapiens](NCBI Reference Sequence: NP_005976.2) <400> 15 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Leu Gln 65 70 75 80 Glu Ser Pro Arg Val Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Val Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Glu 130 135 140 Ala Lys Asp Leu Gln Ala Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Cys Pro Arg 260 <210> 16 <211> 1722 <212> DNA <213> Artificial Sequence <220> <223> Wild type Snail1(whole nucleotide sequence)_Homo sapiens snail family zinc finger 1 (SNAI1), mRNA <400> 16 attcattgcg ccgcggcacg gcctagcgag tggttcttct gcgctactgc tgcgcgaatc 60 ggcgacccca gtgcctcgac cactatgccg cgctctttcc tcgtcaggaa gccctccgac 120 cccaatcgga agcctaacta cagcgagctg caggactcta atccagagtt taccttccag 180 cagccctacg accaggccca cctgctggca gccatcccac ctccggagat cctcaacccc 240 accgcctcgc tgccaatgct catctgggac tctgtcctgg cgccccaagc ccagccaatt 300 gcctgggcct cccttcggct ccaggagagt cccagggtgg cagagctgac ctccctgtca 360 gatgaggaca gtgggaaagg ctcccagccc cccagcccac cctcaccggc tccttcgtcc 420 ttctcctcta cttcagtctc ttccttggag gccgaggcct atgctgcctt cccaggcttg 480 ggccaagtgc ccaagcagct ggcccagctc tctgaggcca aggatctcca ggctcgaaag 540 gccttcaact gcaaatactg caacaaggaa tacctcagcc tgggtgccct caagatgcac 600 atccgaagcc acacgctgcc ctgcgtctgc ggaacctgcg ggaaggcctt ctctaggccc 660 tggctgctac aaggccatgt ccggacccac actggcgaga agcccttctc ctgtccccac 720 tgcagccgtg ccttcgctga ccgctccaac ctgcgggccc acctccagac ccactcagat 780 gtcaagaagt accagtgcca ggcgtgtgct cggaccttct cccgaatgtc cctgctccac 840 aagcaccaag agtccggctg ctcaggatgt ccccgctgac cctcgaggct ccctcttcct 900 ctccatacct gcccctgcct gacagccttc cccagctcca gcaggaagga ccccacatcc 960 ttctcactgc catggaattc cctcctgagt gccccacttc tggccacatc agccccacag 1020 gactttgatg aagaccattt tctggttctg tgtcctctgc ctgggctctg gaagaggcct 1080 tcccatggcc atttctgtgg agggagggca gctggccccc agccctgggg gattcctgag 1140 ctggcctgtc tgcgtgggtt tttgtatcca gagctgtttg gatacagctg ctttgagcta 1200 caggacaaag gctgacagac tcactgggaa gctcccaccc cactcagggg accccactcc 1260 cctcacacac acccccccac aaggaaccct caggccaccc tccacgaggt gtgactaact 1320 atgcaataat ccacccccag gtgcagcccc agggcctgcg gaggcggtgg cagactagag 1380 tctgagatgc cccgagccca ggcagctatt tcagcctcct gtttggtggg gtggcacctg 1440 tttcccgggc aatttaacaa tgtctgaaaa gggactgtga gtaatggctg tcacttgtcg 1500 ggggcccaag tggggtgctc tggtctgacc gatgtgtctc ccagaactat tctgggggcc 1560 cgacaggtgg gcctgggagg aagatgttta catttttaaa ggtacactgg tatttatatt 1620 tcaaacattt tgtatcaagg aaacgttttg tatagttata tgtacagttt attgatattc 1680 aataaagcag ttaatttata tattaaaaaa aaaaaaaaaa aa 1722 <210> 17 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for EPRS <400> 17 atccgcgtta gagctgattt 20 <210> 18 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for EPRS <400> 18 taatgggaac tcccttgagc 20 <210> 19 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for SNAIL1 <400> 19 cctccctgtc agatgaggac 20 <210> 20 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for SNAIL1 <400> 20 ccaggctgag gtattccttg 20 <210> 21 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for GAPDH <400> 21 caatgacccc ttcattgacc 20 <210> 22 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for GAPDH <400> 22 gacaagcttc ccgttctcag 20 <210> 23 <211> 21 <212> RNA <213> Artificial Sequence <220> <223> siRNA Sense sequence for EPRS (si-EPRS) <400> 23 cgaugucagc auuuccguut t 21 <210> 24 <211> 21 <212> RNA <213> Artificial Sequence <220> <223> siRNA Antisense sequence for EPRS (si-EPRS) <400> 24 aacggaaaug cugacaucgt c 21 <210> 25 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS EAW93309.1 <400> 25 Met Ala Thr Leu Ser Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Ala 1 5 10 15 Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val Glu 20 25 30 Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe Thr 35 40 45 Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr Ala 50 55 60 Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His Trp 65 70 75 80 Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr Ser 85 90 95 Thr Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val 100 105 110 Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys 115 120 125 Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro Val 130 135 140 His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe Gln 145 150 155 160 Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val Ala 165 170 175 Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala 180 185 190 Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr 195 200 205 Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln 210 215 220 Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro 225 230 235 240 Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala 245 250 255 Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe 260 265 270 Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala 275 280 285 Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln 290 295 300 Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu Gln 305 310 315 320 Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys Cys 325 330 335 Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp 340 345 350 Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn 355 360 365 Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp 370 375 380 Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp 385 390 395 400 Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys 405 410 415 Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu 420 425 430 Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly 435 440 445 Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly 450 455 460 Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser 465 470 475 480 Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys 485 490 495 Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys 500 505 510 Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys Glu 515 520 525 Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val Trp 530 535 540 Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe Ser 545 550 555 560 Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile Thr 565 570 575 Lys Ile His Lys Asn Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala Lys 580 585 590 Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Val Thr Trp 595 600 605 Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Val Ile Cys Val Thr 610 615 620 Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe 625 630 635 640 Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu Gly 645 650 655 Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln 660 665 670 Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro 675 680 685 Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp Gly 690 695 700 His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Val 705 710 715 720 Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro Thr 725 730 735 Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Pro Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser Ser 805 810 815 Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ser Pro Lys Ala Lys 835 840 845 Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln 865 870 875 880 Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu Glu 885 890 895 Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val Asp 915 920 925 Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn Gln 980 985 990 Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr His 1025 1030 1035 1040 Asp Ile Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile Trp 1045 1050 1055 Glu Ala Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Ser Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Ile Pro Gly Glu Lys 1250 1255 1260 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn Asp 1315 1320 1325 Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala 1380 1385 1390 Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile Gln 1395 1400 1405 Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1475 1480 1485 Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 26 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS CAI45949.1 <400> 26 Met Ala Thr Leu Ser Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Thr Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Val Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Val Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro 725 730 735 Thr Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser 805 810 815 Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ser Pro Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu 885 890 895 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val 915 920 925 Asp Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Glu Ser 930 935 940 Leu Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn 980 985 990 Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 His Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile 1045 1050 1055 Trp Glu Ala Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Ser Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Val Thr Val Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Ile Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 Gln Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly 1425 1430 1435 1440 Arg Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 27 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_001172425.1 <400> 27 Met Ala Thr Leu Ser Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Val Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro 725 730 735 Thr Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser 805 810 815 Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu 885 890 895 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val 915 920 925 Asp Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn 980 985 990 Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 His Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile 1045 1050 1055 Trp Glu Ala Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Ser Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Ile Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Arg Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 Gln Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 28 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_003807230.1 <400> 28 Met Ala Thr Leu Ser Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Val Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro 725 730 735 Thr Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser 805 810 815 Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu 885 890 895 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val 915 920 925 Asp Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn 980 985 990 Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 His Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile 1045 1050 1055 Trp Glu Ala Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Ser Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Ile Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Arg Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 Gln Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 29 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_009439782.1 <400> 29 Met Ala Thr Leu Ser Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Val Pro 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Pro 530 535 540 Glu Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Ile Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Leu Asn Ile Thr Lys Ile His Lys Asn Ala Asp Gly 580 585 590 Lys Ile Ile Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr His Ala Leu 610 615 620 Pro Ile Pro Val Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys 690 695 700 Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Ala Thr Lys Asn Glu Thr Ser 725 730 735 Ala Pro Phe Lys Glu Arg Pro Thr Pro Ser Leu Asn Asn Asn Cys Thr 740 745 750 Thr Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Val Gln Gly 755 760 765 Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Pro Lys Glu Asp Val 770 775 780 Asp Ala Ala Val Lys Gln Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Glu Ile Gly 805 810 815 Gln Asn Ile Ser Ser Asn Ser Ser Ala Ser Ile Leu Glu Ser Lys Ser 820 825 830 Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys 835 840 845 Ala Glu Lys Ala Pro Lys Ala Lys Ile Asn Glu Ala Val Glu Cys Leu 850 855 860 Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Ile 865 870 875 880 Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp Ser Ser Pro Thr Arg 885 890 895 Asn Ser Glu Pro Ala Gly Leu Glu Thr Pro Glu Ala Lys Val Leu Phe 900 905 910 Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Thr Glu 915 920 925 Lys Ala Pro Lys Asp Gln Val Asp Ile Ala Val Gln Glu Leu Leu Gln 930 935 940 Leu Lys Ala Gln Tyr Lys Ser Leu Ile Gly Val Glu Tyr Lys Pro Val 945 950 955 960 Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu 965 970 975 Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Gln 980 985 990 Arg Lys Asp Pro Ser Lys Asn Gln Gly Gly Gly Leu Ser Ser Ser Gly 995 1000 1005 Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ser Glu Met Ile Glu Tyr His Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Ala Tyr Ala Ile Trp Glu Ala Ile Lys Asp Phe Phe Asp 1060 1065 1070 Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe 1075 1080 1085 Val Ser Gln Ser Ala Leu Glu Lys Glu Lys Thr His Val Ala Asp Phe 1090 1095 1100 Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala 1105 1110 1115 1120 Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala 1125 1130 1135 Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Lys Leu Asn 1140 1145 1150 Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe 1155 1160 1165 Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala 1170 1175 1180 Thr Met Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr 1185 1190 1195 1200 Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg 1205 1210 1215 Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Ile 1220 1225 1230 Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Gly Thr Ser 1235 1240 1245 His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Val Phe Glu 1250 1255 1260 Asp Pro Lys Ile Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp 1265 1270 1275 1280 Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp 1285 1290 1295 Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val 1300 1305 1310 Ile Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Lys Glu 1315 1320 1325 Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Arg Val 1330 1335 1340 Asn Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp 1345 1350 1355 1360 Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val 1365 1370 1375 Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp 1380 1385 1390 Thr Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys Leu 1395 1400 1405 Gln Ala Ile Leu Glu Asp Ile Gln Val Thr Leu Phe Thr Arg Ala Ser 1410 1415 1420 Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp Phe 1425 1430 1435 1440 Gln Lys Ile Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln 1460 1465 1470 Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Val Cys 1490 1495 1500 Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 30 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_003265132.1 <400> 30 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro 725 730 735 Thr Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Phe Val 740 745 750 Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser 805 810 815 Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu 885 890 895 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val 915 920 925 Asp Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Ala Gln Arg Lys Asp Pro Ser Lys Asn 980 985 990 Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 His Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile 1045 1050 1055 Trp Glu Ala Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Asn Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Val Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 Gln Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 31 <211> 1522 <212> PRT <213> Artificial Sequence <220> <223> EPRS EAW93310.1 <400> 31 Met Ala His Cys Gly His Asp Phe Pro Gly Ser Ser Ser Pro Ala Thr 1 5 10 15 Ser Ala Pro Trp Val Ala Gly Thr Thr Gly Ala Leu Leu Ala Val Glu 20 25 30 His Val Lys Asp Asp Val Ser Ile Ser Val Glu Glu Gly Lys Glu Asn 35 40 45 Ile Leu His Val Ser Glu Asn Val Ile Phe Thr Asp Val Asn Ser Ile 50 55 60 Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr Ala Gly Leu Tyr Gly Ser 65 70 75 80 Asn Leu Met Glu His Thr Glu Ile Asp His Trp Leu Glu Phe Ser Ala 85 90 95 Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr Ser Thr Ile Asn Glu Leu 100 105 110 Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn Ser Leu Ser 115 120 125 Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn Ala Ala Trp 130 135 140 Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro Val His Val Lys Arg Trp 145 150 155 160 Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe Gln Ser Val Gly Thr Lys 165 170 175 Trp Asp Val Ser Thr Thr Lys Ala Arg Val Ala Pro Glu Lys Lys Gln 180 185 190 Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met Gly Lys Val 195 200 205 Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His Ile Gly His 210 215 220 Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn Phe Lys Gly 225 230 235 240 Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys Glu Lys Glu 245 250 255 Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu His Ile Lys 260 265 270 Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile Met Lys 275 280 285 Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr 290 295 300 Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Ile Glu Ser Lys 305 310 315 320 His Arg Lys Asn Pro Ile Glu Lys Asn Leu Gln Met Trp Glu Glu Met 325 330 335 Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile 340 345 350 Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg 355 360 365 Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr 370 375 380 Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val 385 390 395 400 Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe 405 410 415 Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu 420 425 430 Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu 435 440 445 Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg 450 455 460 Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly 465 470 475 480 Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn 485 490 495 Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Val Ile Asp Pro 500 505 510 Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val 515 520 525 Asn Val Pro Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro 530 535 540 Lys Asn Pro Glu Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val 545 550 555 560 Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val 565 570 575 Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile Thr Lys Ile His Lys Asn 580 585 590 Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn 595 600 605 Lys Asp Tyr Lys Lys Thr Thr Lys Val Thr Trp Leu Ala Glu Thr Thr 610 615 620 His Ala Leu Pro Ile Pro Val Ile Cys Val Thr Tyr Glu His Leu Ile 625 630 635 640 Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn 645 650 655 Lys Asn Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys 660 665 670 Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe 675 680 685 Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu 690 695 700 Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met 705 710 715 720 Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Val Glu Ala Thr Lys Asn 725 730 735 Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro Thr Pro Ser Leu Asn Asn 740 745 750 Asn Cys Thr Thr Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala 755 760 765 Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Pro Lys 770 775 780 Glu Asp Val Asp Ala Ala Val Lys Gln Leu Leu Ser Leu Lys Ala Glu 785 790 795 800 Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala 805 810 815 Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser Ser Ala Ser Ile Leu Glu 820 825 830 Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg 835 840 845 Lys Leu Lys Ala Glu Lys Ser Pro Lys Ala Lys Ile Asn Glu Ala Val 850 855 860 Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys 865 870 875 880 Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp Ser Ser 885 890 895 Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu Glu Thr Pro Glu Ala Lys 900 905 910 Val Leu Phe Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu 915 920 925 Lys Thr Glu Lys Ala Pro Lys Asp Gln Val Asp Ile Ala Val Gln Glu 930 935 940 Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Ile Gly Val Glu Tyr 945 950 955 960 Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys 965 970 975 Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn 980 985 990 Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn Gln Gly Gly Gly Leu Ser 995 1000 1005 Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu 1010 1015 1020 Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln 1025 1030 1035 1040 Val Ile Thr Lys Ser Glu Met Ile Glu Tyr His Asp Ile Ser Gly Cys 1045 1050 1055 Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile Trp Glu Ala Ile Lys Asp 1060 1065 1070 Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe 1075 1080 1085 Pro Met Phe Val Ser Gln Ser Ala Leu Glu Lys Glu Lys Thr His Val 1090 1095 1100 Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr 1105 1110 1115 1120 Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met 1125 1130 1135 Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile 1140 1145 1150 Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro 1155 1160 1165 Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser 1170 1175 1180 Ala Phe Ala Thr Met Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu 1185 1190 1195 1200 Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val 1205 1210 1215 Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr 1220 1225 1230 Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly 1235 1240 1245 Gly Thr Ser His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile 1250 1255 1260 Val Phe Glu Asp Pro Lys Ile Pro Gly Glu Lys Gln Phe Ala Tyr Gln 1265 1270 1275 1280 Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val 1285 1290 1295 His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val 1300 1305 1310 Gln Val Val Ile Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu 1315 1320 1325 Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu 1330 1335 1340 Leu Ser Val Asn Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser 1345 1350 1355 1360 Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg 1365 1370 1375 Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val 1380 1385 1390 Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu 1395 1400 1405 Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile Gln Val Thr Leu Phe Thr 1410 1415 1420 Arg Ala Ser Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met 1425 1430 1435 1440 Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly Lys Ile Val Gln Ile Pro 1445 1450 1455 Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala 1460 1465 1470 Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser 1475 1480 1485 Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys 1490 1495 1500 Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg 1505 1510 1515 1520 Ser Tyr <210> 32 <211> 1440 <212> PRT <213> Artificial Sequence <220> <223> EPRS CAA38224.1 <400> 32 Met Glu His Thr Glu Ile Asp His Trp Leu Glu Phe Ser Ala Thr Lys 1 5 10 15 Leu Ser Ser Cys Asp Ser Phe Thr Ser Thr Ile Asn Glu Leu Asn His 20 25 30 Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn Ser Leu Ser Leu Ala 35 40 45 Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn Ala Ala Trp Gln Glu 50 55 60 Gln Leu Lys Gln Lys Lys Ala Pro Val His Val Lys Arg Trp Phe Gly 65 70 75 80 Phe Leu Glu Ala Gln Gln Ala Phe Gln Ser Val Gly Thr Lys Trp Asp 85 90 95 Val Ser Thr Thr Lys Ala Arg Val Ala Pro Glu Lys Lys Gln Asp Val 100 105 110 Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met Gly Lys Val Thr Val 115 120 125 Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His Ile Gly His Ala Lys 130 135 140 Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn Phe Lys Gly Lys Leu 145 150 155 160 Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys Glu Lys Glu Asp Phe 165 170 175 Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu His Ile Lys Pro Asp 180 185 190 Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile Met Lys Tyr Ala 195 200 205 Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala 210 215 220 Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Ile Glu Ser Lys His Arg 225 230 235 240 Lys Asn Pro Ile Glu Lys Asn Leu Gln Met Trp Glu Glu Met Lys Lys 245 250 255 Gly Ser Gln Phe Gly His Ser Cys Cys Leu Arg Ala Lys Ile Asp Met 260 265 270 Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys 275 280 285 Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr Pro Thr 290 295 300 Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val Thr His 305 310 315 320 Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp 325 330 335 Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser 340 345 350 Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu Thr Trp 355 360 365 Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg Phe Pro 370 375 380 Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly Leu Lys 385 390 395 400 Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn Met Glu 405 410 415 Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Val Ile Asp Pro Val Ala 420 425 430 Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Val 435 440 445 Pro Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn 450 455 460 Pro Glu Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Ile 465 470 475 480 Glu Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe 485 490 495 Ile Asn Trp Gly Asn Leu Asn Ile Thr Lys Ile His Lys Asn Ala Asp 500 505 510 Gly Lys Ile Ile Ser Leu Asp Ala Lys Phe Asn Leu Glu Asn Lys Asp 515 520 525 Tyr Lys Lys Thr Thr Lys Val Thr Trp Leu Ala Glu Thr Thr His Ala 530 535 540 Leu Pro Ile Pro Val Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys 545 550 555 560 Pro Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn 565 570 575 Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu 580 585 590 Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys 595 600 605 Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro 610 615 620 Cys Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr 625 630 635 640 Ser Gly Ser Lys Glu Lys Thr Lys Val Glu Ala Thr Lys Asn Glu Thr 645 650 655 Ser Ala Pro Phe Lys Glu Arg Pro Thr Pro Ser Leu Asn Asn Asn Cys 660 665 670 Thr Thr Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Val Gln 675 680 685 Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Pro Lys Glu Asp 690 695 700 Val Asp Ala Ala Val Lys Gln Leu Leu Ser Leu Lys Ala Glu Tyr Lys 705 710 715 720 Glu Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Glu Ile 725 730 735 Gly Gln Asn Ile Ser Ser Asn Ser Ser Ala Ser Ile Leu Glu Ser Lys 740 745 750 Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu 755 760 765 Lys Ala Glu Lys Ser Pro Lys Ala Lys Ile Asn Glu Ala Val Glu Cys 770 775 780 Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr 785 790 795 800 Ile Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp Ser Ser Pro Thr 805 810 815 Arg Asn Ser Glu Pro Ala Gly Leu Glu Thr Pro Glu Ala Lys Val Leu 820 825 830 Phe Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Thr 835 840 845 Glu Lys Ala Pro Lys Asp Gln Val Asp Ile Ala Val Gln Glu Leu Leu 850 855 860 Gln Leu Lys Ala Gln Tyr Lys Ser Leu Ile Gly Val Glu Tyr Lys Pro 865 870 875 880 Val Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys 885 890 895 Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly 900 905 910 Gln Arg Lys Asp Pro Ser Lys Asn Gln Gly Gly Gly Leu Ser Ser Ser 915 920 925 Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu 930 935 940 Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile 945 950 955 960 Thr Lys Ser Glu Met Ile Glu Tyr His Asp Ile Ser Gly Cys Tyr Ile 965 970 975 Leu Arg Pro Trp Ala Tyr Ala Ile Trp Glu Ala Ile Lys Asp Phe Phe 980 985 990 Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met 995 1000 1005 Phe Val Ser Gln Ser Ala Leu Glu Lys Glu Lys Thr His Val Ala Asp 1010 1015 1020 Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu 1025 1030 1035 1040 Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro 1045 1050 1055 Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Lys Leu 1060 1065 1070 Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro 1075 1080 1085 Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe 1090 1095 1100 Ala Thr Met Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu 1105 1110 1115 1120 Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly 1125 1130 1135 Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr 1140 1145 1150 Ile Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Gly Thr 1155 1160 1165 Ser His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Val Phe 1170 1175 1180 Glu Asp Pro Lys Ile Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser 1185 1190 1195 1200 Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly 1205 1210 1215 Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val 1220 1225 1230 Val Ile Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Lys 1235 1240 1245 Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser 1250 1255 1260 Val Asn Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly 1265 1270 1275 1280 Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu 1285 1290 1295 Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg 1300 1305 1310 Asp Thr Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys 1315 1320 1325 Leu Gln Ala Ile Leu Glu Asp Ile Gln Val Thr Leu Phe Thr Arg Ala 1330 1335 1340 Ser Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp 1345 1350 1355 1360 Phe Gln Lys Ile Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys 1365 1370 1375 Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp 1380 1385 1390 Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys 1395 1400 1405 Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Val 1410 1415 1420 Cys Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1425 1430 1435 1440 <210> 33 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_010366377.1 <400> 33 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Ile 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Cys Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ser Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ser Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Val Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Thr Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro 725 730 735 Thr Pro Ser Leu Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser Ser 805 810 815 Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Gln Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln 865 870 875 880 Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu Glu 885 890 895 Thr Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala Ser Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val Asp 915 920 925 Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn Gln 980 985 990 Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr His 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile Trp 1045 1050 1055 Glu Ala Ile Lys Asp Phe Phe Asp Ala Gly Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1250 1255 1260 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn Asp 1315 1320 1325 Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala 1380 1385 1390 Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile His 1395 1400 1405 Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1475 1480 1485 Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 34 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005540939.1 <400> 34 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Ile 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Cys Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Thr Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Val Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Thr Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Pro Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro 725 730 735 Thr Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser 805 810 815 Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Gln Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu 885 890 895 Glu Thr Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val 915 920 925 Asp Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn 980 985 990 Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 His Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile 1045 1050 1055 Trp Glu Ala Ile Lys Asp Phe Phe Asp Ala Gly Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Asn Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 His Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 35 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007986597.1 <400> 35 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Ile 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Pro Gly Leu Tyr Gly Cys Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Thr Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Val Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Thr Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Ala Phe Lys Glu Arg Pro 725 730 735 Thr Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser 805 810 815 Ser Ala Ser Ile Leu Glu Ser Arg Ser Leu Tyr Asp Gln Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Asn Arg Asn Ser Glu Pro Ala Gly Leu 885 890 895 Glu Thr Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val 915 920 925 Asp Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Ser Gln Arg Lys Asp Pro Ser Lys Asn 980 985 990 Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 His Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile 1045 1050 1055 Trp Glu Ala Ile Lys Asp Phe Phe Asp Ala Gly Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Asn Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 His Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 36 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005540938.1 <400> 36 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Ile 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Cys Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Thr Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Val Pro 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Pro 530 535 540 Glu Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Val Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Leu Asn Ile Thr Lys Ile His Lys Asn Thr Asp Gly 580 585 590 Lys Ile Ile Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr His Ala Pro 610 615 620 Pro Val Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys 690 695 700 Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Ala Thr Lys Asn Glu Thr Ser 725 730 735 Ala Pro Phe Lys Glu Arg Pro Thr Pro Ser Leu Asn Asn Asn Cys Thr 740 745 750 Thr Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Val Gln Gly 755 760 765 Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Pro Lys Glu Asp Ile 770 775 780 Asp Ala Ala Val Lys Gln Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Glu Ile Gly 805 810 815 Gln Asn Ile Ser Ser Asn Ser Ser Ala Ser Ile Leu Glu Ser Lys Ser 820 825 830 Leu Tyr Asp Gln Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys 835 840 845 Ala Glu Lys Ala Pro Lys Ala Lys Ile Asn Glu Ala Val Glu Cys Leu 850 855 860 Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Ile 865 870 875 880 Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp Ser Ser Pro Thr Arg 885 890 895 Asn Ser Glu Pro Ala Gly Leu Glu Thr Pro Glu Ala Lys Leu Leu Phe 900 905 910 Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Thr Glu 915 920 925 Lys Ala Pro Lys Asp Gln Val Asp Ile Ala Val Gln Glu Leu Leu Gln 930 935 940 Leu Lys Ala Gln Tyr Lys Ser Leu Ile Gly Val Glu Tyr Lys Pro Val 945 950 955 960 Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu 965 970 975 Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Gln 980 985 990 Arg Lys Asp Pro Ser Lys Asn Gln Gly Gly Gly Leu Ser Ser Ser Gly 995 1000 1005 Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ser Glu Met Ile Glu Tyr His Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Ala Tyr Ala Ile Trp Glu Ala Ile Lys Asp Phe Phe Asp 1060 1065 1070 Ala Gly Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe 1075 1080 1085 Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe 1090 1095 1100 Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala 1105 1110 1115 1120 Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala 1125 1130 1135 Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Lys Leu Asn 1140 1145 1150 Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe 1155 1160 1165 Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala 1170 1175 1180 Thr Met Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr 1185 1190 1195 1200 Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Arg Gly Arg 1205 1210 1215 Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val 1220 1225 1230 Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Gly Thr Ser 1235 1240 1245 His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Val Phe Glu 1250 1255 1260 Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp 1265 1270 1275 1280 Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp 1285 1290 1295 Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val 1300 1305 1310 Ile Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Lys Glu 1315 1320 1325 Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Asn Val 1330 1335 1340 Asn Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp 1345 1350 1355 1360 Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val 1365 1370 1375 Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp 1380 1385 1390 Thr Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys Leu 1395 1400 1405 Gln Ala Ile Leu Glu Asp Ile His Val Thr Leu Phe Thr Arg Ala Ser 1410 1415 1420 Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp Phe 1425 1430 1435 1440 Gln Lys Ile Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln 1460 1465 1470 Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Val Cys 1490 1495 1500 Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 37 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007986596.1 <400> 37 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Ile 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Pro Gly Leu Tyr Gly Cys Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Thr Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Val Pro 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Pro 530 535 540 Glu Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Val Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Leu Asn Ile Thr Lys Ile His Lys Asn Thr Asp Gly 580 585 590 Lys Ile Ile Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr His Ala Leu 610 615 620 Pro Val Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys 690 695 700 Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Ala Thr Lys Asn Glu Thr Ser 725 730 735 Ala Ala Phe Lys Glu Arg Pro Thr Pro Ser Leu Asn Asn Asn Cys Thr 740 745 750 Thr Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Val Gln Gly 755 760 765 Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Pro Lys Glu Asp Ile 770 775 780 Asp Ala Ala Val Lys Gln Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Glu Ile Gly 805 810 815 Gln Asn Ile Ser Ser Asn Ser Ser Ala Ser Ile Leu Glu Ser Arg Ser 820 825 830 Leu Tyr Asp Gln Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys 835 840 845 Ala Glu Lys Ala Pro Lys Ala Lys Ile Asn Glu Ala Val Glu Cys Leu 850 855 860 Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Ile 865 870 875 880 Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp Ser Ser Pro Asn Arg 885 890 895 Asn Ser Glu Pro Ala Gly Leu Glu Thr Pro Glu Ala Lys Leu Leu Phe 900 905 910 Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Thr Glu 915 920 925 Lys Ala Pro Lys Asp Gln Val Asp Ile Ala Val Gln Glu Leu Leu Gln 930 935 940 Leu Lys Ala Gln Tyr Lys Ser Leu Ile Gly Val Glu Tyr Lys Pro Val 945 950 955 960 Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu 965 970 975 Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Ser Gln 980 985 990 Arg Lys Asp Pro Ser Lys Asn Gln Gly Gly Gly Leu Ser Ser Ser Gly 995 1000 1005 Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ser Glu Met Ile Glu Tyr His Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Ala Tyr Ala Ile Trp Glu Ala Ile Lys Asp Phe Phe Asp 1060 1065 1070 Ala Gly Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe 1075 1080 1085 Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe 1090 1095 1100 Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala 1105 1110 1115 1120 Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala 1125 1130 1135 Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Lys Leu Asn 1140 1145 1150 Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe 1155 1160 1165 Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala 1170 1175 1180 Thr Met Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr 1185 1190 1195 1200 Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Arg Gly Arg 1205 1210 1215 Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Ile 1220 1225 1230 Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Gly Thr Ser 1235 1240 1245 His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Val Phe Glu 1250 1255 1260 Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp 1265 1270 1275 1280 Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp 1285 1290 1295 Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val 1300 1305 1310 Ile Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Lys Glu 1315 1320 1325 Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Asn Val 1330 1335 1340 Asn Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp 1345 1350 1355 1360 Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val 1365 1370 1375 Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp 1380 1385 1390 Thr Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys Leu 1395 1400 1405 Gln Ala Ile Leu Glu Asp Ile His Val Thr Leu Phe Thr Arg Ala Ser 1410 1415 1420 Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp Phe 1425 1430 1435 1440 Gln Lys Ile Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln 1460 1465 1470 Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Val Cys 1490 1495 1500 Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 38 <211> 1447 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007986598.1 <400> 38 Met Glu His Thr Glu Ile Asp His Trp Leu Glu Phe Ser Ala Thr Lys 1 5 10 15 Leu Ser Ser Cys Asp Ser Phe Thr Ser Ala Ile Asn Glu Leu Asn His 20 25 30 Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn Ser Leu Ser Leu Ala 35 40 45 Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn Ala Ala Trp Gln Glu 50 55 60 Gln Leu Lys Gln Asn Lys Ala Pro Val His Val Lys Arg Trp Phe Gly 65 70 75 80 Phe Leu Glu Ala Gln Gln Thr Phe Gln Ser Val Gly Thr Lys Trp Asp 85 90 95 Val Ser Thr Thr Lys Ala Arg Val Ala Pro Glu Lys Lys Gln Asp Val 100 105 110 Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met Gly Lys Val Thr Val 115 120 125 Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His Ile Gly His Ala Lys 130 135 140 Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn Phe Lys Gly Lys Leu 145 150 155 160 Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys Glu Lys Glu Asp Phe 165 170 175 Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu His Ile Lys Pro Asp 180 185 190 Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile Met Lys Tyr Ala 195 200 205 Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala 210 215 220 Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Ile Glu Ser Lys His Arg 225 230 235 240 Lys Asn Pro Ile Glu Lys Asn Leu Gln Met Trp Glu Glu Met Lys Lys 245 250 255 Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile Asp Met 260 265 270 Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys 275 280 285 Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr Pro Thr 290 295 300 Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val Thr His 305 310 315 320 Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp 325 330 335 Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser 340 345 350 Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu Thr Trp 355 360 365 Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg Phe Pro 370 375 380 Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly Leu Lys 385 390 395 400 Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn Met Glu 405 410 415 Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Leu Arg Ala Leu Cys Lys 420 425 430 Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys 435 440 445 Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys Glu 450 455 460 Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val Trp 465 470 475 480 Tyr Ser Pro Lys Val Phe Val Glu Gly Ala Asp Ala Glu Thr Phe Ser 485 490 495 Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile Thr 500 505 510 Lys Ile His Lys Asn Thr Asp Gly Lys Ile Ile Ser Leu Asp Ala Lys 515 520 525 Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp 530 535 540 Leu Ala Glu Thr Thr His Ala Leu Pro Val Pro Ala Ile Cys Val Thr 545 550 555 560 Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe 565 570 575 Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu Gly 580 585 590 Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln 595 600 605 Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro 610 615 620 Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp Gly 625 630 635 640 His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Val 645 650 655 Glu Ala Thr Lys Asn Glu Thr Ser Ala Ala Phe Lys Glu Arg Pro Thr 660 665 670 Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val Leu 675 680 685 Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala 690 695 700 Lys Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 705 710 715 720 Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 725 730 735 Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser Ser 740 745 750 Ala Ser Ile Leu Glu Ser Arg Ser Leu Tyr Asp Gln Val Ala Ala Gln 755 760 765 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 770 775 780 Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 785 790 795 800 Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln 805 810 815 Ser Ser Asp Ser Ser Pro Asn Arg Asn Ser Glu Pro Ala Gly Leu Glu 820 825 830 Thr Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala Ser Gln Gly Glu 835 840 845 Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val Asp 850 855 860 Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 865 870 875 880 Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys 885 890 895 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 900 905 910 Pro Gln Lys Gln Asn Asp Ser Gln Arg Lys Asp Pro Ser Lys Asn Gln 915 920 925 Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys 930 935 940 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 945 950 955 960 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr His 965 970 975 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile Trp 980 985 990 Glu Ala Ile Lys Asp Phe Phe Asp Ala Gly Ile Lys Lys Leu Gly Val 995 1000 1005 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1010 1015 1020 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1025 1030 1035 1040 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1045 1050 1055 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1060 1065 1070 Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1075 1080 1085 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1090 1095 1100 Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu Glu 1105 1110 1115 1120 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1125 1130 1135 Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1140 1145 1150 Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser Gly 1155 1160 1165 Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe Ser 1170 1175 1180 Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1185 1190 1195 1200 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1205 1210 1215 Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1220 1225 1230 Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr Asn 1235 1240 1245 Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn Asp 1250 1255 1260 Tyr Arg Arg Arg Leu Leu Asn Val Asn Ile Arg Val Arg Ala Asp Leu 1265 1270 1275 1280 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1285 1290 1295 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1300 1305 1310 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala 1315 1320 1325 Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile His 1330 1335 1340 Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1345 1350 1355 1360 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly Lys 1365 1370 1375 Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1380 1385 1390 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1395 1400 1405 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1410 1415 1420 Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr 1425 1430 1435 1440 Thr Leu Phe Gly Arg Ser Tyr 1445 <210> 39 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_003930387.1 <400> 39 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Ser Phe Ala 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Gln Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Val Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Lys Pro 725 730 735 Thr Pro Ser Leu Asn Asn Thr Cys Thr Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Met Ser Ser Asn Ser 805 810 815 Ser Thr Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Leu Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Ala Arg Asn Ser Glu Pro Ala Gly Leu 885 890 895 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ala Gln Gly 900 905 910 Asp Val Val Arg Lys Leu Lys Thr Glu Lys Ala Ser Lys Asp Gln Ile 915 920 925 Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Ile Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn 980 985 990 Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ala Glu Met Ile Glu Tyr 1025 1030 1035 1040 His Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile 1045 1050 1055 Trp Glu Ala Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Ile Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Thr Phe Glu Asp Pro Lys Met Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Ile His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Met Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Asn 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 His Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 40 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_010339237.1 <400> 40 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Ser Phe Ala 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Gln Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Val Pro 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Pro 530 535 540 Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Val Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Leu Asn Ile Thr Lys Ile His Lys Asn Ala Asp Gly 580 585 590 Lys Ile Ile Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr His Ala Leu 610 615 620 Pro Ile Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys 690 695 700 Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Ala Thr Lys Asn Glu Thr Ser 725 730 735 Ala Pro Phe Lys Glu Lys Pro Thr Pro Ser Leu Asn Asn Thr Cys Thr 740 745 750 Thr Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Val Gln Gly 755 760 765 Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Pro Lys Glu Asp Ile 770 775 780 Asp Ala Ala Val Lys Gln Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Glu Ile Gly 805 810 815 Gln Asn Met Ser Ser Asn Ser Ser Thr Ser Ile Leu Glu Ser Lys Ser 820 825 830 Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys 835 840 845 Ala Glu Lys Ala Leu Lys Ala Lys Ile Asn Glu Ala Val Glu Cys Leu 850 855 860 Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Ile 865 870 875 880 Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp Ser Ser Pro Ala Arg 885 890 895 Asn Ser Glu Pro Ala Gly Leu Glu Thr Pro Glu Ala Lys Val Leu Phe 900 905 910 Asp Lys Val Ala Ala Gln Gly Asp Val Val Arg Lys Leu Lys Thr Glu 915 920 925 Lys Ala Ser Lys Asp Gln Ile Asp Thr Ala Val Gln Glu Leu Leu Gln 930 935 940 Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly Ile Glu Tyr Lys Pro Val 945 950 955 960 Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu 965 970 975 Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Gln 980 985 990 Arg Lys Asp Pro Ser Lys Asn Gln Gly Gly Gly Leu Ser Ser Ser Gly 995 1000 1005 Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ala Glu Met Ile Glu Tyr His Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Ala Tyr Ala Ile Trp Glu Ala Ile Lys Asp Phe Phe Asp 1060 1065 1070 Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe 1075 1080 1085 Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Val Ala Asp Phe 1090 1095 1100 Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala 1105 1110 1115 1120 Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala 1125 1130 1135 Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn 1140 1145 1150 Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe 1155 1160 1165 Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala 1170 1175 1180 Thr Ile Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr 1185 1190 1195 1200 Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg 1205 1210 1215 Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val 1220 1225 1230 Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Gly Thr Ser 1235 1240 1245 His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Thr Phe Glu 1250 1255 1260 Asp Pro Lys Met Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp 1265 1270 1275 1280 Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Ile His Gly Asp 1285 1290 1295 Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val 1300 1305 1310 Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Lys Glu 1315 1320 1325 Ala Leu Met Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser Val 1330 1335 1340 Asn Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp 1345 1350 1355 1360 Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val 1365 1370 1375 Gly Pro Arg Asp Met Lys Asn Cys Gln Phe Val Ala Val Arg Arg Asp 1380 1385 1390 Thr Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys Leu 1395 1400 1405 Gln Ala Ile Leu Glu Asp Ile His Val Thr Leu Phe Thr Arg Ala Ser 1410 1415 1420 Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp Phe 1425 1430 1435 1440 Gln Lys Ile Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln 1460 1465 1470 Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Val Cys 1490 1495 1500 Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 41 <211> 1490 <212> PRT <213> Artificial Sequence <220> <223> EPRS EHH50530.1 <400> 41 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Gly Ala Leu 1 5 10 15 Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Ile Glu Glu 20 25 30 Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ile Phe Thr Asp 35 40 45 Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr Ala Gly 50 55 60 Leu Tyr Gly Cys Asn Leu Met Glu His Thr Glu Ile Asp His Trp Leu 65 70 75 80 Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr Ser Ala 85 90 95 Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly 100 105 110 Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly 115 120 125 Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro Val His 130 135 140 Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Thr Phe Gln Ser 145 150 155 160 Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val Ala Pro 165 170 175 Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu 180 185 190 Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu 195 200 205 His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val 210 215 220 Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu 225 230 235 240 Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met 245 250 255 Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu 260 265 270 Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr 275 280 285 Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg 290 295 300 Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu Gln Met 305 310 315 320 Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu 325 330 335 Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro 340 345 350 Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys 355 360 365 Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser 370 375 380 Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg 385 390 395 400 Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro 405 410 415 Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser 420 425 430 Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp 435 440 445 Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met 450 455 460 Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Val Ile Asp Pro 465 470 475 480 Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val 485 490 495 Asn Val Pro Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro 500 505 510 Lys Asn Pro Glu Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val 515 520 525 Phe Val Glu Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val 530 535 540 Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile Thr Lys Ile His Lys Asn 545 550 555 560 Thr Asp Gly Lys Ile Ile Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn 565 570 575 Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr 580 585 590 His Ala Pro Pro Val Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile 595 600 605 Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn 610 615 620 Lys Asn Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys 625 630 635 640 Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe 645 650 655 Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu 660 665 670 Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met 675 680 685 Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Val Glu Ala Thr Lys Asn 690 695 700 Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro Thr Pro Ser Leu Asn Asn 705 710 715 720 Asn Cys Thr Thr Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala 725 730 735 Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Pro Lys 740 745 750 Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu Ser Leu Lys Ala Glu 755 760 765 Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala 770 775 780 Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser Ser Ala Ser Ile Leu Glu 785 790 795 800 Ser Lys Ser Leu Tyr Asp Gln Val Ala Ala Gln Gly Glu Val Val Arg 805 810 815 Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Ile Asn Glu Ala Val 820 825 830 Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys 835 840 845 Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp Ser Ser 850 855 860 Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu Glu Thr Pro Glu Ala Lys 865 870 875 880 Leu Leu Phe Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu 885 890 895 Lys Thr Glu Lys Ala Pro Lys Asp Gln Val Asp Ile Ala Val Gln Glu 900 905 910 Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Ile Gly Val Glu Tyr 915 920 925 Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys 930 935 940 Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn 945 950 955 960 Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn Gln Gly Gly Gly Leu Ser 965 970 975 Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu 980 985 990 Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln 995 1000 1005 Val Ile Thr Lys Ser Glu Met Ile Glu Tyr His Asp Val Ser Gly Cys 1010 1015 1020 Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile Trp Glu Ala Ile Lys Asp 1025 1030 1035 1040 Phe Phe Asp Ala Gly Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe 1045 1050 1055 Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Ile 1060 1065 1070 Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr 1075 1080 1085 Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met 1090 1095 1100 Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile 1105 1110 1115 1120 Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro 1125 1130 1135 Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser 1140 1145 1150 Ala Phe Ala Thr Met Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu 1155 1160 1165 Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val 1170 1175 1180 Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr 1185 1190 1195 1200 Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly 1205 1210 1215 Gly Thr Ser His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile 1220 1225 1230 Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln 1235 1240 1245 Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val 1250 1255 1260 His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val 1265 1270 1275 1280 Gln Val Val Ile Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu 1285 1290 1295 Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu 1300 1305 1310 Leu Asn Val Asn Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser 1315 1320 1325 Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg 1330 1335 1340 Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val 1345 1350 1355 1360 Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu 1365 1370 1375 Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile His Val Thr Leu Phe Thr 1380 1385 1390 Arg Ala Ser Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met 1395 1400 1405 Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly Lys Ile Val Gln Ile Pro 1410 1415 1420 Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala 1425 1430 1435 1440 Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser 1445 1450 1455 Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys 1460 1465 1470 Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg 1475 1480 1485 Ser Tyr 1490 <210> 42 <211> 1527 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_002808297.1 <400> 42 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Ile 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Cys Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Thr Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Val Pro 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Pro 530 535 540 Glu Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Val Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Leu Asn Ile Thr Lys Ile His Lys Asn Thr Asp Gly 580 585 590 Lys Ile Ile Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr His Ala Pro 610 615 620 Pro Val Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys 690 695 700 Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Ala Thr Lys Asn Glu Thr Ser 725 730 735 Ala Pro Phe Lys Glu Arg Pro Thr Pro Ser Leu Asn Asn Asn Cys Thr 740 745 750 Thr Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Val Gln Gly 755 760 765 Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Pro Lys Glu Asp Ile 770 775 780 Asp Ala Ala Val Lys Gln Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Glu Ile Gly 805 810 815 Gln Asn Ile Ser Ser Asn Ser Ser Ala Ser Ile Leu Glu Ser Lys Ser 820 825 830 Leu Tyr Asp Gln Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys 835 840 845 Ala Glu Lys Ala Pro Lys Ala Lys Ile Asn Glu Ala Val Glu Cys Leu 850 855 860 Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Ile 865 870 875 880 Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp Ser Ser Pro Thr Arg 885 890 895 Asn Ser Glu Pro Ala Gly Leu Glu Thr Pro Glu Ala Lys Leu Leu Phe 900 905 910 Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Thr Glu 915 920 925 Lys Ala Pro Lys Asp Gln Val Asp Ile Ala Val Gln Glu Leu Leu Gln 930 935 940 Leu Lys Ala Gln Tyr Lys Ser Leu Ile Gly Val Glu Tyr Lys Pro Val 945 950 955 960 Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu 965 970 975 Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Gln 980 985 990 Arg Lys Asp Pro Ser Lys Asn Gln Gly Gly Gly Leu Ser Ser Ser Gly 995 1000 1005 Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ser Glu Met Ile Glu Tyr His Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Ala Tyr Ala Ile Trp Glu Ala Ile Lys Asp Phe Phe Asp 1060 1065 1070 Ala Gly Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe 1075 1080 1085 Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe 1090 1095 1100 Ala Pro Glu Val Ser Glu Leu Leu Phe Tyr Phe Phe Ala Val Thr Tyr 1105 1110 1115 1120 Ser Leu Lys Gly Glu Ile Lys Pro Xaa Met Thr Val Ser Ile Phe Pro 1125 1130 1135 Cys Leu Ser Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1140 1145 1150 Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1155 1160 1165 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1170 1175 1180 Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu Glu 1185 1190 1195 1200 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1205 1210 1215 Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1220 1225 1230 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1235 1240 1245 Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe Ser 1250 1255 1260 Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1265 1270 1275 1280 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1285 1290 1295 Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1300 1305 1310 Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr Asn 1315 1320 1325 Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn Asp 1330 1335 1340 Tyr Arg Arg Arg Leu Leu Asn Val Asn Ile Arg Val Arg Ala Asp Leu 1345 1350 1355 1360 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1365 1370 1375 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1380 1385 1390 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala 1395 1400 1405 Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile His 1410 1415 1420 Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1425 1430 1435 1440 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly Lys 1445 1450 1455 Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1460 1465 1470 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1475 1480 1485 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1490 1495 1500 Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr 1505 1510 1515 1520 Thr Leu Phe Gly Arg Ser Tyr 1525 <210> 43 <211> 1499 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_009185375.1 <400> 43 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Ile 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Cys Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Leu Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Ile Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Asn Ser Glu Phe Met Ala Ala Val Val Asn Ile Ser Leu 545 550 555 560 Asn Thr Phe Asn Leu Leu Ser Arg Asn Thr Asp Gly Lys Ile Ile Ser 565 570 575 Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr 580 585 590 Lys Ile Thr Trp Leu Ala Glu Thr Thr His Ala Leu Pro Val Pro Ala 595 600 605 Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys 610 615 620 Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu 625 630 635 640 Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile 645 650 655 Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu 660 665 670 Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr 675 680 685 Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu 690 695 700 Lys Thr Lys Val Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys 705 710 715 720 Glu Arg Pro Thr Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp 725 730 735 Ser Leu Val Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg 740 745 750 Glu Leu Lys Ala Lys Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val 755 760 765 Lys Gln Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln 770 775 780 Glu Tyr Lys Pro Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser 785 790 795 800 Ser Asn Ser Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Gln 805 810 815 Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala 820 825 830 Pro Lys Ala Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys 835 840 845 Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro 850 855 860 Pro Leu Ser Gln Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro 865 870 875 880 Ala Gly Leu Glu Thr Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala 885 890 895 Ser Gln Gly Glu Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys 900 905 910 Asp Gln Ile Asp Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln 915 920 925 Tyr Lys Ser Leu Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly 930 935 940 Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu 945 950 955 960 Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro 965 970 975 Ser Lys Asn Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly 980 985 990 Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu 995 1000 1005 Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met 1010 1015 1020 Ile Glu Tyr His Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala 1025 1030 1035 1040 Tyr Ala Ile Trp Glu Ala Ile Lys Asp Phe Phe Asp Ala Gly Ile Lys 1045 1050 1055 Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly 1060 1065 1070 Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val 1075 1080 1085 Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala 1090 1095 1100 Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp 1105 1110 1115 1120 Val Gln Ser His Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn 1125 1130 1135 Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg 1140 1145 1150 Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu 1155 1160 1165 Ala Glu Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr 1170 1175 1180 Glu Glu Leu Leu Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys 1185 1190 1195 1200 Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile 1205 1210 1215 Ser Ala Ser Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly 1220 1225 1230 Gln Asn Phe Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr 1235 1240 1245 Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr 1250 1255 1260 Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu 1265 1270 1275 1280 Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys 1285 1290 1295 Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala 1300 1305 1310 Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Asn Val Asn Ile Arg Val 1315 1320 1325 Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His 1330 1335 1340 Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp 1345 1350 1355 1360 Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys 1365 1370 1375 Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu 1380 1385 1390 Glu Asp Ile His Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys 1395 1400 1405 Thr His Met Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu 1410 1415 1420 Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys 1425 1430 1435 1440 Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro 1445 1450 1455 Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro 1460 1465 1470 Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro 1475 1480 1485 Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1490 1495 <210> 44 <211> 1510 <212> PRT <213> Artificial Sequence <220> <223> EPRS EHH15538.1 <400> 44 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Gly Ala Leu 1 5 10 15 Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Ile Glu Glu 20 25 30 Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ile Phe Thr Asp 35 40 45 Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr Ala Gly 50 55 60 Leu Tyr Gly Cys Asn Leu Met Glu His Thr Glu Ile Asp His Trp Leu 65 70 75 80 Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr Ser Ala 85 90 95 Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly 100 105 110 Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly 115 120 125 Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro Val His 130 135 140 Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Thr Phe Gln Ser 145 150 155 160 Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val Ala Pro 165 170 175 Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu 180 185 190 Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu 195 200 205 His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val 210 215 220 Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu 225 230 235 240 Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met 245 250 255 Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu 260 265 270 Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr 275 280 285 Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg 290 295 300 Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu Gln Met 305 310 315 320 Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu 325 330 335 Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro 340 345 350 Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys 355 360 365 Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser 370 375 380 Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg 385 390 395 400 Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro 405 410 415 Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser 420 425 430 Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp 435 440 445 Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met 450 455 460 Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg 465 470 475 480 Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys 485 490 495 Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu 500 505 510 Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys Glu Val 515 520 525 Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val Trp Tyr 530 535 540 Ser Pro Lys Val Phe Val Glu Gly Ala Asp Ala Glu Thr Phe Ser Glu 545 550 555 560 Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile Thr Lys 565 570 575 Ile His Lys Asn Thr Asp Gly Lys Ile Ile Ser Leu Asp Ala Lys Leu 580 585 590 Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu 595 600 605 Ala Glu Thr Thr His Ala Pro Pro Val Pro Ala Ile Cys Val Thr Tyr 610 615 620 Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys 625 630 635 640 Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu Gly Asp 645 650 655 Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg 660 665 670 Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr 675 680 685 Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp Gly His 690 695 700 Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Val Glu 705 710 715 720 Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro Thr Pro 725 730 735 Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val Leu Tyr 740 745 750 Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys 755 760 765 Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu Ser 770 775 780 Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro Gly 785 790 795 800 Asn Arg Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser Ser Ala 805 810 815 Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Gln Val Ala Ala Gln Gly 820 825 830 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Ile 835 840 845 Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu 850 855 860 Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln Ser 865 870 875 880 Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu Glu Thr 885 890 895 Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala Ser Gln Gly Glu Val 900 905 910 Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val Asp Ile 915 920 925 Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Ile 930 935 940 Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp 945 950 955 960 Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro 965 970 975 Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn Gln Gly 980 985 990 Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys 995 1000 1005 Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp 1010 1015 1020 Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr His Asp 1025 1030 1035 1040 Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile Trp Glu 1045 1050 1055 Ala Ile Lys Asp Phe Phe Asp Ala Gly Ile Lys Lys Leu Gly Val Glu 1060 1065 1070 Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys Glu 1075 1080 1085 Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg 1090 1095 1100 Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser 1105 1110 1115 1120 Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg 1125 1130 1135 Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu 1140 1145 1150 Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln 1155 1160 1165 Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu Glu Val 1170 1175 1180 Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala 1185 1190 1195 1200 Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly 1205 1210 1215 Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly Arg 1220 1225 1230 Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe Ser Lys 1235 1240 1245 Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln 1250 1255 1260 Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val 1265 1270 1275 1280 Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg 1285 1290 1295 Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr Asn Ala 1300 1305 1310 Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr 1315 1320 1325 Arg Arg Arg Leu Leu Asn Val Asn Ile Arg Val Arg Ala Asp Leu Arg 1330 1335 1340 Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly 1345 1350 1355 1360 Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys Gln 1365 1370 1375 Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala Glu 1380 1385 1390 Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile His Val 1395 1400 1405 Thr Leu Phe Thr Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 1410 1415 1420 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ile 1425 1430 1435 1440 Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys 1445 1450 1455 Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met 1460 1465 1470 Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln 1475 1480 1485 Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr Thr 1490 1495 1500 Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 45 <211> 1495 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004028474.1 <400> 45 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Ile Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Ser Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Lys Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Val Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Val Ser Thr Thr Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Lys Ser Val Val 245 250 255 Met Thr Leu Cys Gln Lys Ser Xaa Ile Lys Val Thr Gly Leu Pro Asn 260 265 270 Thr Phe Met Asn Phe Thr Ile Ile Lys Pro Gly Val Pro His Leu Gly 275 280 285 Ser Ile Thr Ile Glu Lys Asn Leu Gln Met Trp Glu Glu Met Lys Lys 290 295 300 Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile Asp Met 305 310 315 320 Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys 325 330 335 Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr Pro Thr 340 345 350 Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val Thr His 355 360 365 Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp 370 375 380 Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser 385 390 395 400 Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu Thr Trp 405 410 415 Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg Phe Pro 420 425 430 Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly Leu Lys 435 440 445 Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn Met Glu 450 455 460 Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Leu Arg Ala Leu Cys Lys 465 470 475 480 Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys 485 490 495 Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys Glu 500 505 510 Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val Trp 515 520 525 Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe Leu 530 535 540 Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile Thr 545 550 555 560 Lys Ile His Lys Asn Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala Lys 565 570 575 Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp 580 585 590 Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Val Ile Cys Val Thr 595 600 605 Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe 610 615 620 Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu Gly 625 630 635 640 Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln 645 650 655 Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro 660 665 670 Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp Gly 675 680 685 His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Val 690 695 700 Glu Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Arg Pro Thr 705 710 715 720 Pro Ser Leu Asn Asn Asn Cys Thr Thr Ser Glu Asp Ser Leu Val Leu 725 730 735 Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala 740 745 750 Lys Lys Ala Pro Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu Leu 755 760 765 Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 770 775 780 Gly Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser Ser 785 790 795 800 Ala Ser Ile Leu Glu Ser Lys Pro Leu Tyr Asp Glu Val Ala Ala Gln 805 810 815 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 820 825 830 Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 835 840 845 Glu Lys Thr Gly Lys Glu Tyr Ile Ser Gly Gln Pro Pro Leu Ser Gln 850 855 860 Ser Ser Asp Ser Ser Pro Thr Arg Asn Ser Glu Pro Ala Gly Leu Glu 865 870 875 880 Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly Glu 885 890 895 Val Val Arg Lys Leu Lys Thr Glu Lys Ala Pro Lys Asp Gln Val Asp 900 905 910 Ile Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 915 920 925 Ile Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys 930 935 940 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 945 950 955 960 Pro Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn Gln 965 970 975 Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys 980 985 990 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 995 1000 1005 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr His 1010 1015 1020 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile Trp 1025 1030 1035 1040 Glu Ala Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1045 1050 1055 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Ser Ala Leu Glu Lys 1060 1065 1070 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1075 1080 1085 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1090 1095 1100 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1105 1110 1115 1120 Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1125 1130 1135 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1140 1145 1150 Gln Glu Gly His Ser Ala Phe Ala Thr Met Glu Glu Ala Ala Glu Glu 1155 1160 1165 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1170 1175 1180 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1185 1190 1195 1200 Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser Gly 1205 1210 1215 Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe Ser 1220 1225 1230 Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1235 1240 1245 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1250 1255 1260 Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1265 1270 1275 1280 Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr Asn 1285 1290 1295 Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn Asp 1300 1305 1310 Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp Leu 1315 1320 1325 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1330 1335 1340 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1345 1350 1355 1360 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala 1365 1370 1375 Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile Gln 1380 1385 1390 Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1395 1400 1405 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly Lys 1410 1415 1420 Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1425 1430 1435 1440 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1445 1450 1455 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1460 1465 1470 Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr 1475 1480 1485 Thr Leu Phe Gly Arg Ser Tyr 1490 1495 <210> 46 <211> 1494 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_008983438.1 <400> 46 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Thr Leu Arg Val Ser Glu Leu Lys Cys Trp 35 40 45 Ser Ala Phe Phe Phe Tyr Leu Leu Cys Ser Gln Ile Asp His Trp Leu 50 55 60 Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Ser Phe Thr Ser Ala 65 70 75 80 Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly 85 90 95 Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly 100 105 110 Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro Val His 115 120 125 Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe Gln Ser 130 135 140 Val Gly Thr Gln Trp Asp Val Ser Thr Ala Lys Ala Arg Val Ala Pro 145 150 155 160 Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu 165 170 175 Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu 180 185 190 His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val 195 200 205 Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu 210 215 220 Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met 225 230 235 240 Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu 245 250 255 Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr 260 265 270 Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg 275 280 285 Ile Glu Ser Lys His Arg Lys Asn Pro Ile Glu Lys Asn Leu Gln Met 290 295 300 Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu 305 310 315 320 Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro 325 330 335 Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys 340 345 350 Phe Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser 355 360 365 Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg 370 375 380 Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Val Gly Ile Arg Lys Pro 385 390 395 400 Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser 405 410 415 Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp 420 425 430 Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met 435 440 445 Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg 450 455 460 Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys 465 470 475 480 Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu 485 490 495 Val Ile Pro Val Asn Val Pro Gly Ala Gln Glu Glu Met Lys Glu Val 500 505 510 Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val Trp Tyr 515 520 525 Ser Pro Lys Val Phe Val Glu Gly Ala Asp Ala Glu Thr Phe Ser Glu 530 535 540 Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Leu Asn Ile Thr Lys 545 550 555 560 Ile His Lys Asn Ala Ala Gly Lys Ile Thr Ser Leu Asp Ala Lys Leu 565 570 575 Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu 580 585 590 Ala Glu Thr Thr His Ala Leu Pro Ile Pro Ala Ile Cys Val Thr Tyr 595 600 605 Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys 610 615 620 Gln Tyr Val Asn Lys Asp Ser Lys His Glu Glu Leu Met Leu Gly Asp 625 630 635 640 Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg 645 650 655 Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr 660 665 670 Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp Gly His 675 680 685 Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Val Glu 690 695 700 Ala Thr Lys Asn Glu Thr Ser Ala Pro Phe Lys Glu Glu Pro Thr Pro 705 710 715 720 Ser Leu Asp Asn Thr Cys Thr Ala Ser Glu Asp Ser Leu Val Leu Tyr 725 730 735 Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys 740 745 750 Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu Ser 755 760 765 Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro Gly 770 775 780 Asn Pro Pro Ala Glu Ile Gly Gln Asn Ile Ser Ser Asn Ser Ser Ala 785 790 795 800 Ser Ile Leu Gln Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly 805 810 815 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Ile 820 825 830 Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu 835 840 845 Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln Ser 850 855 860 Ser Asp Ser Ser Pro Ala Arg Asn Ser Glu Pro Ala Gly Leu Glu Thr 865 870 875 880 Pro Glu Ala Lys Val Leu Phe Asn Gln Val Ala Ser Gln Gly Asp Val 885 890 895 Val Arg Lys Leu Lys Thr Glu Lys Ala Ser Lys Asp Gln Ile Asp Thr 900 905 910 Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr 915 920 925 Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp 930 935 940 Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro 945 950 955 960 Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Pro Ser Lys Asn Gln Gly 965 970 975 Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys 980 985 990 Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp 995 1000 1005 Trp Tyr Ser Gln Val Ile Thr Lys Ala Glu Met Ile Glu Tyr His Asp 1010 1015 1020 Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile Trp Glu 1025 1030 1035 1040 Ala Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu 1045 1050 1055 Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys Glu 1060 1065 1070 Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg 1075 1080 1085 Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser 1090 1095 1100 Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg 1105 1110 1115 1120 Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu 1125 1130 1135 Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln 1140 1145 1150 Glu Gly His Ser Ala Phe Ala Thr Ile Glu Glu Ala Ala Glu Glu Val 1155 1160 1165 Leu Gln Ile Leu Asp Leu Tyr Ala Gln Ile Tyr Glu Glu Leu Leu Ala 1170 1175 1180 Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly 1185 1190 1195 1200 Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly Arg 1205 1210 1215 Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe Ser Lys 1220 1225 1230 Met Phe Glu Ile Met Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln 1235 1240 1245 Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val 1250 1255 1260 Met Thr Met Ile His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg 1265 1270 1275 1280 Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala 1285 1290 1295 Leu Ser Glu Glu Asp Lys Glu Ala Leu Met Ala Lys Cys Asn Asp Tyr 1300 1305 1310 Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp Leu Arg 1315 1320 1325 Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly 1330 1335 1340 Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Asn Cys Gln 1345 1350 1355 1360 Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ser Glu 1365 1370 1375 Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile His Val 1380 1385 1390 Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val Val 1395 1400 1405 Ala Asn Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly Lys Ile 1410 1415 1420 Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys 1425 1430 1435 1440 Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met 1445 1450 1455 Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln 1460 1465 1470 Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr Thr 1475 1480 1485 Leu Phe Gly Arg Ser Tyr 1490 <210> 47 <211> 1513 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_008053500.1 <400> 47 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Ile Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Val Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr 85 90 95 Ser Ala Ile Ser Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Gln Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Ile Ser Thr Thr Lys Ala Lys Val 165 170 175 Thr Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Ile Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Val Glu Arg Glu 290 295 300 Gln Arg Val Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Leu Asp Ile Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Ile Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Thr Gly Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ser Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Ala Glu Thr Ile Lys Asn Glu Lys Thr Ser Ala Ser Phe Lys Glu Arg 725 730 735 Pro Ala Pro Thr Leu Asn Asn Pro Cys Ala Ile Ser Gly Asp Ser Leu 740 745 750 Ala Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu 755 760 765 Lys Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln 770 775 780 Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu Arg Thr Ser Gln Glu Tyr 785 790 795 800 Lys Pro Gly Asn Pro Pro Ala Gly Ile Ala Gln Asn Ile Ser Phe Lys 805 810 815 Ser Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala 820 825 830 Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Thr Ser Lys 835 840 845 Ala Lys Ile Asn Glu Ala Val Glu Tyr Leu Leu Ser Leu Lys Ala Gln 850 855 860 Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu 865 870 875 880 Ser Gln Ser Ser Asp Ser Ser Pro Ser Arg Thr Ser Glu Pro Thr Asp 885 890 895 Pro Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Thr Gln 900 905 910 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln 915 920 925 Val Asp Thr Ala Val Gln Glu Leu Leu Lys Leu Lys Ala Leu Tyr Lys 930 935 940 Ser Leu Thr Gly Val Asp Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu 945 950 955 960 Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln 965 970 975 Asn Lys Ala Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Ser Ser Lys 980 985 990 Asn Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly 995 1000 1005 Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn 1010 1015 1020 Leu Ala Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu 1025 1030 1035 1040 Tyr Tyr Asp Ile Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser 1045 1050 1055 Ile Trp Glu Ser Ile Lys Glu Phe Phe Asp Ala Gly Ile Lys Lys Leu 1060 1065 1070 Gly Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu 1075 1080 1085 Glu Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp 1090 1095 1100 Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg 1105 1110 1115 1120 Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln 1125 1130 1135 Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val 1140 1145 1150 Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe 1155 1160 1165 Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr Val Glu Glu Ala Ala 1170 1175 1180 Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Asp 1185 1190 1195 1200 Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys 1205 1210 1215 Phe Ala Gly Gly Asp Tyr Thr Thr Thr Leu Glu Ala Phe Ile Ser Ala 1220 1225 1230 Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn 1235 1240 1245 Phe Ser Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly 1250 1255 1260 Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr 1265 1270 1275 1280 Ile Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu 1285 1290 1295 Pro Pro Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile 1300 1305 1310 Thr Asn Ala Leu Ser Glu Glu Asp Arg Glu Ala Leu Ile Ala Lys Cys 1315 1320 1325 Asn Asp Tyr Arg Arg Arg Leu Leu Asn Val Asn Ile Arg Val Lys Val 1330 1335 1340 Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu 1345 1350 1355 1360 Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys 1365 1370 1375 Ser Phe Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr 1380 1385 1390 Val Thr Glu Asn Glu Ala Glu Ile Lys Leu Gln Ala Ile Leu Glu Asp 1395 1400 1405 Ile His Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His 1410 1415 1420 Met Val Val Ala Asn Thr Met Glu Glu Phe Gln Lys Ile Leu Asp Ser 1425 1430 1435 1440 Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp 1445 1450 1455 Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Ile Glu Pro Gly Ala 1460 1465 1470 Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys 1475 1480 1485 Glu Leu Gln Pro Gly Ala Lys Cys Ile Cys Gly Lys Asn Pro Ala Lys 1490 1495 1500 Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 48 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_001488980.1 <400> 48 Met Ala Thr Leu Cys Leu Thr Val Asn Thr Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Thr Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Ala Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Ala 85 90 95 Ser Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Phe Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Ser Gln Ser Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Leu Val Gly Thr Lys Trp Asn Val Ser Ala Ala Lys Ala Lys Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Leu Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Arg Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala Arg Ala Leu Pro Val Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Arg Glu Ala Pro Cys Val Leu Ile Phe Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Thr Thr Pro Leu Lys Glu Arg Pro 725 730 735 Ala Pro Ser Leu Asn Asn Thr Cys Ala Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Lys Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Ala Val Val Gln Asn Val Ser Ser Lys Ser 805 810 815 Ser Ala Thr Val Leu Glu Ser Lys Ala Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Asn Glu Ala Val Glu Ser Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Thr Arg Ser Ser Glu Pro Cys Gly Pro 885 890 895 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ala 930 935 940 Leu Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Val Glu Asp 945 950 955 960 Arg Asp Lys Arg Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Ser Gln Lys Gln Asn Asp Gly Gln Lys Lys Asp Pro Phe Arg Asn 980 985 990 Gln Gly Ser Gly Leu Ser Ser Asp Gly Thr Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ser Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Asn Val Asn Ile Arg Val Arg Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 His Val Asn Leu Phe Thr Lys Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Val Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Arg Cys Val Cys Gly Lys Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 49 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRSXP_008541056.1 <400> 49 Met Ala Thr Leu Cys Leu Thr Val Asn Thr Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Thr Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Ala Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Ala 85 90 95 Ser Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Phe Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Ser Gln Ser Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Leu Val Gly Thr Lys Trp Asn Val Ser Ala Ala Lys Ala Lys Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Leu Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Arg Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala Arg Ala Leu Pro Val Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Arg Glu Ala Pro Cys Val Leu Ile Phe Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Thr Lys Asn Glu Thr Thr Thr Pro Leu Lys Glu Arg Pro 725 730 735 Ala Pro Ser Leu Asn Asn Thr Cys Ala Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Lys Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Ala Val Val Gln Asn Val Ser Ser Lys Ser 805 810 815 Ser Ala Thr Val Leu Glu Ser Lys Ala Leu Tyr Asp Glu Val Ala Val 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Asn Glu Ala Val Glu Ser Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Asp Tyr Ile Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Thr Arg Ser Ser Glu Pro Cys Gly Pro 885 890 895 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ala 930 935 940 Leu Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Val Glu Asp 945 950 955 960 Arg Asp Lys Arg Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Ser Gln Lys Gln Asn Asp Gly Gln Lys Lys Asp Pro Phe Arg Asn 980 985 990 Gln Gly Ser Gly Leu Ser Ser Asp Gly Thr Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ser Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Asn Val Asn Ile Arg Val Arg Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 His Val Asn Leu Phe Thr Lys Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Val Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Arg Cys Val Cys Gly Lys Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 50 <211> 1509 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_849468.1 <400> 50 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Ile Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Thr Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Ile Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Ile Ser Ala Thr Lys Ala Lys Val 165 170 175 Thr Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Ser Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Ala Lys Ile His Lys Asn Ala Asp Gly Lys Ile Glu Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala His Ala Leu Pro Ile Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Glu Pro Thr Thr Pro Phe Lys Glu Lys Ala 725 730 735 Gly Pro Ser Leu Leu Asn Thr Cys Ala Thr Phe Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Val Val Pro Asn Val Ser Ser Thr Ser Ser 805 810 815 Ala Ser Ile Val Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Val Val Cys Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln 865 870 875 880 Ser Ser His Ser Thr Ala Gly Ser Glu Ser Ser Gly Pro Glu Ala Pro 885 890 895 Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly Glu Val Val 900 905 910 Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Val Ala 915 920 925 Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly 930 935 940 Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys 945 950 955 960 Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Leu 965 970 975 Lys Gln Ser Asp Gly Gln Lys Lys Asp Ser Tyr Lys Asn Gln Gly Ser 980 985 990 Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln 995 1000 1005 Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp 1010 1015 1020 Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val 1025 1030 1035 1040 Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp Glu Ser 1045 1050 1055 Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn 1060 1065 1070 Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys Glu Lys 1075 1080 1085 Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser 1090 1095 1100 Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr Ser Glu 1105 1110 1115 1120 Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp 1125 1130 1135 Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe 1140 1145 1150 Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu 1155 1160 1165 Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu Val Leu 1170 1175 1180 Gln Ile Leu Asp Leu Tyr Ala Lys Val Tyr Glu Glu Leu Leu Ala Ile 1185 1190 1195 1200 Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly 1205 1210 1215 Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala 1220 1225 1230 Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser Lys Met 1235 1240 1245 Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe 1250 1255 1260 Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met 1265 1270 1275 1280 Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val 1285 1290 1295 Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala Leu 1300 1305 1310 Ser Glu Glu Asp Arg Asp Ala Leu Ile Ile Lys Cys Asn Asp Tyr Arg 1315 1320 1325 Arg Arg Leu Leu Ser Val Asn Val Arg Val Lys Val Asp Leu Arg Asp 1330 1335 1340 Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val 1345 1350 1355 1360 Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe 1365 1370 1375 Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala Asp Asn 1380 1385 1390 Glu Ala Glu Thr Lys Leu Lys Ala Ile Leu Glu Asp Ile His Val Asn 1395 1400 1405 Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val Val Ala 1410 1415 1420 Asn Thr Met Glu Asp Phe Gln Lys Met Leu Asp Ser Gly Lys Ile Ala 1425 1430 1435 1440 Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys 1445 1450 1455 Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly 1460 1465 1470 Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro 1475 1480 1485 Gly Ala Lys Cys Ile Cys Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu 1490 1495 1500 Phe Gly Arg Ser Tyr 1505 <210> 51 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004013652.1 <400> 51 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Gly Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Phe Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Ser Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Glu Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Glu His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Ile Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Glu Ala Ser Thr Pro Leu Lys Thr Arg Ser 725 730 735 Ala Pro Ser Leu Ser Thr Cys Ala Thr Ser Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Thr Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Ala Ala Ala Gly Gln Asn Val Ser Ser Lys Ser Ser 805 810 815 Ser Asn Ile Val Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ser Ser Gln 865 870 875 880 Ser Ser Asp Ser Ser Pro Pro Arg Ser Ser Glu Pro Ser Gly Pro Glu 885 890 895 Thr Pro Glu Ala Lys Leu Leu Phe Asp Arg Val Ala Ser Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp 915 920 925 Thr Ala Val Gln Asp Leu Leu Gln Leu Lys Ala Gln Tyr Arg Ser Leu 930 935 940 Thr Gly Val Asp Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Glu Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Gln Asn Asp Thr Gln Lys Lys Asp Phe Ser Lys Asn Gln 980 985 990 Gly Ser Gly Leu Ser Pro Asn Gly Ala Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Arg Lys Glu Asp Ser Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr His 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Ala Gly Glu Lys 1250 1255 1260 Gln Leu Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Thr Met Val His Gly Asp Asn Val Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Leu Ala Lys Cys Asn Asp 1315 1320 1325 Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Val Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Asn Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala 1380 1385 1390 Glu Asn Glu Ala Glu Thr Lys Leu Arg Ala Ile Leu Glu Asp Ile His 1395 1400 1405 Thr Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1475 1480 1485 Pro Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 52 <211> 1516 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005640882.1 <400> 52 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Ile Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Thr Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Ile Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Ile Ser Ala Thr Lys Ala Lys Val 165 170 175 Thr Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Ile Pro 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Pro 530 535 540 Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Ser Lys Val Phe Ile Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Leu Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Ile Asn Ile Ala Lys Ile His Lys Asn Ala Asp Gly 580 585 590 Lys Ile Glu Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Ala His Ala Leu 610 615 620 Pro Ile Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys 690 695 700 Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Thr Thr Lys Asn Glu Pro Thr 725 730 735 Thr Pro Phe Lys Glu Lys Ala Gly Pro Ser Leu Leu Asn Thr Cys Ala 740 745 750 Thr Phe Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Ala Gln Gly 755 760 765 Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp Ile 770 775 780 Asp Ala Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Val Val Pro 805 810 815 Asn Val Ser Ser Thr Ser Ser Ala Ser Ile Val Glu Ser Lys Ser Leu 820 825 830 Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Cys Lys Leu Lys Ala 835 840 845 Glu Lys Ala Pro Lys Ala Lys Val Asn Glu Ala Val Glu Cys Leu Leu 850 855 860 Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro 865 870 875 880 Gly Gln Pro Pro Leu Ser Gln Ser Ser His Ser Thr Ala Gly Ser Glu 885 890 895 Ser Ser Gly Pro Glu Ala Pro Glu Ala Lys Val Leu Phe Asp Lys Val 900 905 910 Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser 915 920 925 Lys Asp Gln Val Asp Val Ala Val Gln Glu Leu Leu Gln Leu Lys Ala 930 935 940 Gln Tyr Lys Ser Leu Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Thr 945 950 955 960 Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser 965 970 975 Glu Lys Gln Asn Lys Pro Leu Lys Gln Ser Asp Gly Gln Lys Lys Asp 980 985 990 Ser Tyr Lys Asn Gln Gly Ser Gly Leu Ser Ser Ser Gly Ala Gly Glu 995 1000 1005 Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys 1010 1015 1020 Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu 1025 1030 1035 1040 Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp 1045 1050 1055 Ala Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile 1060 1065 1070 Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln 1075 1080 1085 Gly Ala Leu Glu Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu 1090 1095 1100 Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile 1105 1110 1115 1120 Ala Val Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys 1125 1130 1135 Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys 1140 1145 1150 Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr 1155 1160 1165 Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu 1170 1175 1180 Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Lys Val 1185 1190 1195 1200 Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu 1205 1210 1215 Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe 1220 1225 1230 Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu 1235 1240 1245 Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys 1250 1255 1260 Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr 1265 1270 1275 1280 Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp Asn Met Gly 1285 1290 1295 Leu Val Leu Pro Pro Arg Val Ala Ser Val Gln Val Val Val Ile Pro 1300 1305 1310 Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile 1315 1320 1325 Ile Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Val Arg 1330 1335 1340 Val Lys Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn 1345 1350 1355 1360 His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg 1365 1370 1375 Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu 1380 1385 1390 Lys Leu Thr Val Ala Asp Asn Glu Ala Glu Thr Lys Leu Lys Ala Ile 1395 1400 1405 Leu Glu Asp Ile His Val Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu 1410 1415 1420 Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Met 1425 1430 1435 1440 Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp 1445 1450 1455 Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu 1460 1465 1470 Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys 1475 1480 1485 Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Ile Cys Gly Lys Asn 1490 1495 1500 Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 53 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005981363.1 <400> 53 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Gly Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Phe Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Ser Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Glu Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Glu His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Ile Ser 675 680 685 Pro Tyr Ser Cys Ile Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Glu Ala Ser Thr Pro Leu Lys Thr Gly Ser 725 730 735 Ala Pro Ser Leu Ser Thr Cys Ala Thr Ser Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Thr Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Ala Ala Ala Gly Gln Asn Ile Ser Ser Lys Ser Ser 805 810 815 Ser Asn Ile Val Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ser Ser Gln 865 870 875 880 Ser Ser Asp Ser Ser Pro Pro Arg Ser Ser Glu Pro Ser Gly Pro Glu 885 890 895 Thr Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala Ser Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp 915 920 925 Thr Ala Val Gln Asp Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Thr Gly Val Asp Tyr Lys Pro Ile Ser Ala Thr Gly Ala Glu Glu Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Gln Asn Asp Thr Gln Lys Lys Asp Ser Ser Lys Asn Gln 980 985 990 Gly Ser Gly Leu Ser Pro Asn Gly Ala Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Arg Lys Glu Asp Ser Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr His 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Ala Gly Glu Lys 1250 1255 1260 Gln Leu Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Thr Met Val His Gly Asp Asn Val Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Leu Ala Lys Cys Asn Asp 1315 1320 1325 Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Val Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Asn Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala 1380 1385 1390 Glu Asn Glu Ala Glu Thr Lys Leu His Ala Ile Leu Glu Asp Ile His 1395 1400 1405 Ile Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1475 1480 1485 Pro Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 54 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005690536.1 <400> 54 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Thr Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Gly Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Phe Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Ser Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Glu Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Glu His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Ile Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Glu Ala Ser Thr Pro Leu Lys Thr Arg Ser 725 730 735 Ala Pro Ser Leu Gly Thr Cys Ala Thr Ala Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Thr Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ala Leu Lys Ala Asp Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Ala Ala Ala Gly Gln Asn Ile Ser Ser Lys Ser Ser 805 810 815 Ser Asn Ile Val Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ser Ser Gln 865 870 875 880 Ser Ser Asp Ser Ser Pro Pro Arg Ser Ser Glu Pro Ser Gly Pro Glu 885 890 895 Thr Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala Ser Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp 915 920 925 Thr Ala Val Gln Asp Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Thr Gly Val Asp Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Glu Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Gln Asn Asp Thr Gln Lys Lys Asp Ser Ser Lys Asn Gln 980 985 990 Gly Ser Gly Leu Ser Pro Asn Gly Ala Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Arg Lys Glu Asp Ser Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr His 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Ala Gly Glu Lys 1250 1255 1260 Gln Leu Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Thr Met Val His Gly Asp Asn Val Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Leu Ala Lys Cys Asn Asp 1315 1320 1325 Tyr Arg Arg Arg Leu Leu Ser Val Ser Ile Arg Val Arg Val Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Asn Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala 1380 1385 1390 Glu Asn Glu Ala Glu Thr Lys Leu Arg Ala Val Leu Glu Asp Ile His 1395 1400 1405 Ile Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1475 1480 1485 Pro Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 55 <211> 1509 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_002920053.1 <400> 55 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Ala 165 170 175 Ile Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Gln Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Ile Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile Asn Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Arg Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Glu Pro Thr Thr Pro Phe Lys Glu Arg Pro 725 730 735 Gly Pro Ser Leu Asp Asn Thr Cys Ala Pro Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Val Val Gln Asp Val Ser Ser Lys Ser Ser 805 810 815 Ala Ser Thr Val Asp Ser Lys Ser Leu Tyr Glu Glu Ile Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln 865 870 875 880 Ser Ser Asp Ser Thr Asn Ser Ser Glu Pro Ser Gly Pro Glu Thr Pro 885 890 895 Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly Glu Val Val 900 905 910 Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Thr Ala 915 920 925 Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly 930 935 940 Val Glu Tyr Lys Pro Val Ser Val Thr Gly Ala Glu Asp Lys Asp Lys 945 950 955 960 Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln 965 970 975 Lys Gln Ser Asp Gly Gln Lys Lys Asp Ser Phe Lys Ser Gln Gly Ser 980 985 990 Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln 995 1000 1005 Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp 1010 1015 1020 Tyr Ser Gln Val Ile Thr Lys Ser Glu Leu Ile Glu Tyr Tyr Asp Val 1025 1030 1035 1040 Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp Glu Ser 1045 1050 1055 Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn 1060 1065 1070 Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys Glu Lys 1075 1080 1085 Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser 1090 1095 1100 Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr Ser Glu 1105 1110 1115 1120 Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp 1125 1130 1135 Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe 1140 1145 1150 Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu 1155 1160 1165 Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu Val Leu 1170 1175 1180 Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile 1185 1190 1195 1200 Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly 1205 1210 1215 Asp Tyr Thr Thr Thr Leu Glu Val Phe Ile Ser Ala Ser Gly Arg Ala 1220 1225 1230 Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser Lys Met 1235 1240 1245 Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe 1250 1255 1260 Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met 1265 1270 1275 1280 Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val 1285 1290 1295 Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala Leu 1300 1305 1310 Ser Glu Glu Asp Arg Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg 1315 1320 1325 Lys Arg Leu Leu Ser Val Asn Ile Arg Val Arg Val Asp Leu Arg Asp 1330 1335 1340 Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val 1345 1350 1355 1360 Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe 1365 1370 1375 Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala Asp Asn 1380 1385 1390 Glu Ala Glu Thr Lys Leu His Ala Leu Leu Glu Asp Ile His Val Asn 1395 1400 1405 Leu Phe Thr Arg Ala Ser Lys Asp Leu Lys Thr His Met Val Val Ala 1410 1415 1420 Asn Thr Met Glu Asp Phe Gln Gln Met Leu Asp Ser Gly Lys Ile Val 1425 1430 1435 1440 Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys 1445 1450 1455 Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly 1460 1465 1470 Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro 1475 1480 1485 Gly Ala Lys Cys Ile Cys Gly Arg Asn Pro Ala Lys Tyr Tyr Thr Leu 1490 1495 1500 Phe Gly Arg Ser Tyr 1505 <210> 56 <211> 1518 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005981362.1 <400> 56 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Gly Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Phe Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Val Pro Val Asn Val Pro 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Pro 530 535 540 Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Ser Lys Val Phe Ile Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Leu Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Asn Ala Glu Gly 580 585 590 Lys Ile Val Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Glu His Ala Leu 610 615 620 Pro Val Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Ile Ser Pro Tyr Ser Cys Ile Glu Ala Pro Cys 690 695 700 Ile Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Thr Thr Lys Asn Glu Ala Ser 725 730 735 Thr Pro Leu Lys Thr Gly Ser Ala Pro Ser Leu Ser Thr Cys Ala Thr 740 745 750 Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp 755 760 765 Val Val Arg Glu Leu Lys Ala Lys Lys Ala Thr Lys Glu Asp Ile Asp 770 775 780 Ala Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys 785 790 795 800 Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Ala Ala Gly Gln 805 810 815 Asn Ile Ser Ser Lys Ser Ser Ser Asn Ile Val Glu Ser Lys Ser Leu 820 825 830 Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala 835 840 845 Glu Lys Ala Pro Lys Ala Lys Val Asn Glu Ala Val Glu Cys Leu Leu 850 855 860 Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro 865 870 875 880 Gly Gln Pro Pro Ser Ser Gln Ser Ser Asp Ser Ser Pro Pro Arg Ser 885 890 895 Ser Glu Pro Ser Gly Pro Glu Thr Pro Glu Ala Lys Leu Leu Phe Asp 900 905 910 Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys 915 920 925 Ala Ser Lys Asp Gln Val Asp Thr Ala Val Gln Asp Leu Leu Gln Leu 930 935 940 Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val Asp Tyr Lys Pro Ile Ser 945 950 955 960 Ala Thr Gly Ala Glu Glu Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn 965 970 975 Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Thr Gln Lys 980 985 990 Lys Asp Ser Ser Lys Asn Gln Gly Ser Gly Leu Ser Pro Asn Gly Ala 995 1000 1005 Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala 1010 1015 1020 Arg Lys Glu Asp Ser Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys 1025 1030 1035 1040 Ser Glu Met Ile Glu Tyr His Asp Val Ser Gly Cys Tyr Ile Leu Arg 1045 1050 1055 Pro Trp Ala Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala 1060 1065 1070 Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe Val 1075 1080 1085 Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe Ala 1090 1095 1100 Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu 1105 1110 1115 1120 Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr 1125 1130 1135 Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Lys Leu Asn Gln 1140 1145 1150 Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu 1155 1160 1165 Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr 1170 1175 1180 Phe Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala 1185 1190 1195 1200 Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys 1205 1210 1215 Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu 1220 1225 1230 Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His 1235 1240 1245 His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Val Phe Glu Asp 1250 1255 1260 Pro Lys Thr Ala Gly Glu Lys Gln Leu Ala Tyr Gln Asn Ser Trp Gly 1265 1270 1275 1280 Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp Asn 1285 1290 1295 Val Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val Val 1300 1305 1310 Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala 1315 1320 1325 Leu Leu Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn 1330 1335 1340 Ile Arg Val Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys 1345 1350 1355 1360 Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly 1365 1370 1375 Pro Arg Asp Met Lys Asn Cys Gln Phe Val Ala Val Arg Arg Asp Thr 1380 1385 1390 Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys Leu His 1395 1400 1405 Ala Ile Leu Glu Asp Ile His Ile Asn Leu Phe Thr Arg Ala Ser Glu 1410 1415 1420 Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp Phe Gln 1425 1430 1435 1440 Lys Glu Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu 1445 1450 1455 Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp 1460 1465 1470 Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro 1475 1480 1485 Phe Lys Pro Leu Cys Glu Leu Pro Pro Gly Ala Lys Cys Val Cys Gly 1490 1495 1500 Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 57 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004271072.1 <400> 57 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Ser His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Glu Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Thr Ser Lys Ala Lys Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Leu Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asp Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Thr Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Arg Asn Ala Glu Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Glu Arg Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Ile Ser 675 680 685 Pro Tyr Ser Cys Ile Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Glu Ala Ala Thr Pro Leu Lys Thr Met Ser 725 730 735 Ala Pro Ser Leu Asn Asn Thr Cys Ala Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Leu Gly Asn Pro Pro Ala Ala Thr Val Gln Asn Val Ser Ser Thr Ser 805 810 815 Ser Ser Asp Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Ala Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ser Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Ser Arg Ser Ser Glu Pro Arg Gly Pro 885 890 895 Glu Thr Pro Glu Ala Lys Ser Leu Phe Asp Lys Val Ala Thr Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Val Asp Tyr Lys Pro Val Ser Ala Asn Gly Ala Glu Glu 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Thr Gln Lys Gln Asn Asp Gly Gln Lys Lys Asp Ser Ser Lys Asn 980 985 990 Gln Gly Ser Gly Leu Ser Ser Ser Gly Pro Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Lys Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Ser Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu His Ala Ile Leu Glu Asp Ile 1395 1400 1405 His Val Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Arg Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Gln Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 58 <211> 1520 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_008053499.1 <400> 58 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Ile Glu His Val Lys Asp Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Val Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr 85 90 95 Ser Ala Ile Ser Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Gln Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asp Ile Ser Thr Thr Lys Ala Lys Val 165 170 175 Thr Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Ile Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Val Glu Arg Glu 290 295 300 Gln Arg Val Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Val Pro 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Leu 530 535 540 Asp Ile Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Ile Phe Ile Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Asn Thr Gly Gly 580 585 590 Lys Ile Val Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr His Ala Leu 610 615 620 Pro Ile Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ser Pro Cys 690 695 700 Ile Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Ala Glu Thr Ile Lys Asn Glu Lys Thr 725 730 735 Ser Ala Ser Phe Lys Glu Arg Pro Ala Pro Thr Leu Asn Asn Pro Cys 740 745 750 Ala Ile Ser Gly Asp Ser Leu Ala Leu Tyr Asn Arg Val Ala Val Gln 755 760 765 Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp 770 775 780 Ile Asp Ala Ala Val Lys Gln Leu Leu Ser Leu Lys Ala Glu Tyr Lys 785 790 795 800 Glu Arg Thr Ser Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Gly Ile 805 810 815 Ala Gln Asn Ile Ser Phe Lys Ser Ser Ala Ser Ile Leu Glu Ser Lys 820 825 830 Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu 835 840 845 Lys Ala Glu Lys Thr Ser Lys Ala Lys Ile Asn Glu Ala Val Glu Tyr 850 855 860 Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr 865 870 875 880 Ile Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp Ser Ser Pro Ser 885 890 895 Arg Thr Ser Glu Pro Thr Asp Pro Glu Thr Pro Glu Ala Lys Val Leu 900 905 910 Phe Asp Lys Val Ala Thr Gln Gly Glu Val Val Arg Lys Leu Lys Ala 915 920 925 Glu Lys Ala Ser Lys Asp Gln Val Asp Thr Ala Val Gln Glu Leu Leu 930 935 940 Lys Leu Lys Ala Leu Tyr Lys Ser Leu Thr Gly Val Asp Tyr Lys Pro 945 950 955 960 Val Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys 965 970 975 Glu Asn Lys Ser Glu Lys Gln Asn Lys Ala Gln Lys Gln Asn Asp Gly 980 985 990 Gln Arg Lys Asp Ser Ser Lys Asn Gln Gly Gly Gly Leu Ser Ser Ser 995 1000 1005 Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu 1010 1015 1020 Glu Ala Lys Lys Glu Glu Asn Leu Ala Glu Trp Tyr Ser Gln Val Ile 1025 1030 1035 1040 Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp Ile Ser Gly Cys Tyr Ile 1045 1050 1055 Leu Arg Pro Trp Ala Tyr Ser Ile Trp Glu Ser Ile Lys Glu Phe Phe 1060 1065 1070 Asp Ala Gly Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met 1075 1080 1085 Phe Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Val Ala Asp 1090 1095 1100 Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu 1105 1110 1115 1120 Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro 1125 1130 1135 Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu 1140 1145 1150 Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro 1155 1160 1165 Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe 1170 1175 1180 Ala Thr Val Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu 1185 1190 1195 1200 Tyr Ala Gln Val Tyr Glu Asp Leu Leu Ala Ile Pro Val Val Lys Gly 1205 1210 1215 Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr 1220 1225 1230 Leu Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr 1235 1240 1245 Ser His His Leu Gly Gln Asn Phe Ser Lys Met Cys Glu Ile Val Phe 1250 1255 1260 Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser 1265 1270 1275 1280 Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly 1285 1290 1295 Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val 1300 1305 1310 Val Ile Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg 1315 1320 1325 Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Asn 1330 1335 1340 Val Asn Ile Arg Val Lys Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly 1345 1350 1355 1360 Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu 1365 1370 1375 Val Gly Pro Arg Asp Met Lys Ser Phe Gln Phe Val Ala Val Arg Arg 1380 1385 1390 Asp Thr Gly Glu Lys Leu Thr Val Thr Glu Asn Glu Ala Glu Ile Lys 1395 1400 1405 Leu Gln Ala Ile Leu Glu Asp Ile His Val Thr Leu Phe Thr Arg Ala 1410 1415 1420 Ser Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Glu 1425 1430 1435 1440 Phe Gln Lys Ile Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys 1445 1450 1455 Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp 1460 1465 1470 Gln Asp Ile Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys 1475 1480 1485 Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Ile 1490 1495 1500 Cys Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 1520 <210> 59 <211> 1566 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004415195.1 <400> 59 Met Thr His Arg Val Val Arg Glu Asp Gly Gly Ser His Asp Ala Gly 1 5 10 15 Glu Gly Arg Arg Leu Arg Gln Arg Gln Ala His Ala His Val Ala Ala 20 25 30 Ser Cys Phe Arg Arg Val Arg Arg Gly Ser Asp Gly Gln Ala Gly Ala 35 40 45 Leu Thr Gly Gly Arg Arg Pro Ala Glu Met Ala Thr Leu Cys Leu Thr 50 55 60 Val Asn Ala Gly Asp Pro Pro Leu Gly Ala Leu Leu Ala Val Glu His 65 70 75 80 Val Lys Asp Asn Val Ser Ile Ser Val Glu Glu Gly Lys Glu Asn Ile 85 90 95 Leu Phe Val Ser Glu Asn Val Val Phe Thr Asp Ile Asn Ser Ile Leu 100 105 110 Arg Tyr Leu Ala Arg Val Ala Ala Thr Ala Gly Leu Tyr Gly Ser Asn 115 120 125 Leu Leu Glu His Thr Glu Ile Asp His Trp Leu Glu Phe Ser Ala Thr 130 135 140 Lys Leu Ser Ser Cys Asn Leu Phe Thr Ser Ala Ile Asn Glu Leu Asn 145 150 155 160 His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn Ser Leu Ser Leu 165 170 175 Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Ser Ala Ala Trp Gln 180 185 190 Glu Gln Leu Lys Gln Asn Lys Ala Pro Val His Val Lys Arg Trp Phe 195 200 205 Gly Phe Leu Glu Ala Gln Gln Ala Phe Gln Ser Val Gly Thr Lys Trp 210 215 220 Asn Val Ser Ala Thr Lys Ala Lys Val Thr Pro Glu Lys Lys Gln Asp 225 230 235 240 Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met Gly Lys Val Thr 245 250 255 Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His Ile Gly His Ala 260 265 270 Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn Phe Lys Gly Lys 275 280 285 Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys Glu Lys Glu Asp 290 295 300 Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu His Ile Lys Pro 305 310 315 320 Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile Met Lys Tyr 325 330 335 Ala Glu Thr Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr Pro 340 345 350 Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Ile Glu Ser Lys His 355 360 365 Arg Lys Asn Ser Ile Glu Lys Asn Leu Gln Met Trp Glu Glu Met Lys 370 375 380 Lys Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile Asp 385 390 395 400 Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys 405 410 415 Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr Pro 420 425 430 Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val Thr 435 440 445 His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr 450 455 460 Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu Tyr 465 470 475 480 Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu Thr 485 490 495 Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg Phe 500 505 510 Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly Leu 515 520 525 Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn Met 530 535 540 Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Val Ile Asp Pro Val 545 550 555 560 Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn 565 570 575 Ile Pro Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys 580 585 590 Asn Pro Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe 595 600 605 Ile Glu Gly Ala Asp Ala Glu Thr Leu Ser Glu Gly Glu Met Val Thr 610 615 620 Phe Ile Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile Asn Lys Asn Ala 625 630 635 640 Asp Gly Lys Ile Val Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys 645 650 655 Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Ala His 660 665 670 Ala Leu Pro Ile Pro Val Ile Cys Val Thr Tyr Glu His Leu Ile Thr 675 680 685 Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys 690 695 700 Asn Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp 705 710 715 720 Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile 725 730 735 Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala 740 745 750 Pro Cys Val Leu Ile Tyr Val Pro Asp Gly His Thr Lys Glu Met Pro 755 760 765 Thr Ser Gly Ser Arg Glu Lys Thr Lys Val Glu Thr Thr Lys Asn Glu 770 775 780 Pro Thr Ala Pro Phe Lys Glu Arg Pro Gly Pro Ser Leu Asp Asn Thr 785 790 795 800 Cys Ala Ser Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Ala 805 810 815 Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu 820 825 830 Asp Ile Asp Ser Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr 835 840 845 Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Val 850 855 860 Val Gln Asn Val Ser Ser Ile Ser Ser Ala Ser Ile Val Asp Ser Arg 865 870 875 880 Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu 885 890 895 Lys Ala Glu Lys Ala Pro Lys Ala Lys Val Asn Glu Ala Val Glu Cys 900 905 910 Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr 915 920 925 Ile Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp Ser Thr Asn Ser 930 935 940 Ser Glu Ala Ser Gly Pro Glu Thr Pro Glu Ser Lys Val Leu Phe Asp 945 950 955 960 Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys 965 970 975 Ala Ser Lys Asp Gln Val Asp Thr Ala Val Gln Glu Leu Leu Gln Leu 980 985 990 Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val Glu Tyr Lys Pro Val Ser 995 1000 1005 Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn 1010 1015 1020 Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Ser Asp Gly Gln Lys 1025 1030 1035 1040 Lys Asp Ser Phe Lys Ser Gln Gly Ser Gly Leu Ser Ser Ser Gly Ala 1045 1050 1055 Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala 1060 1065 1070 Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys 1075 1080 1085 Ser Glu Leu Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg 1090 1095 1100 Pro Trp Ala Tyr Ser Ile Trp Glu Ser Ile Lys Glu Phe Ile Asp Ala 1105 1110 1115 1120 Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe Val 1125 1130 1135 Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe Ala 1140 1145 1150 Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu 1155 1160 1165 Pro Ile Ala Val Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr 1170 1175 1180 Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln 1185 1190 1195 1200 Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu 1205 1210 1215 Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr 1220 1225 1230 Phe Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala 1235 1240 1245 Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys 1250 1255 1260 Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Leu Glu 1265 1270 1275 1280 Val Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His 1285 1290 1295 His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Ile Phe Glu Asp 1300 1305 1310 Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly 1315 1320 1325 Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp Asn 1330 1335 1340 Met Gly Leu Val Leu Pro Pro Arg Val Ala Ser Val Gln Val Met Val 1345 1350 1355 1360 Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg Glu Ala 1365 1370 1375 Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn 1380 1385 1390 Ile Arg Val Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys 1395 1400 1405 Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly 1410 1415 1420 Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr 1425 1430 1435 1440 Gly Glu Lys Leu Thr Val Ala Asp Asn Glu Ala Glu Thr Lys Leu His 1445 1450 1455 Ala Leu Leu Glu Asp Ile His Val Asn Leu Phe Thr Arg Ala Ser Glu 1460 1465 1470 Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp Phe Gln 1475 1480 1485 Lys Met Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu 1490 1495 1500 Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp 1505 1510 1515 1520 Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Val Pro 1525 1530 1535 Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Arg Cys Val Cys Gly 1540 1545 1550 Arg Asn Ala Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1555 1560 1565 <210> 60 <211> 1508 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004324147.1 <400> 60 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Ser His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Glu Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Thr Ser Lys Ala Lys Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys Gln Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Leu Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asp Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Thr Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Arg Asn Ala Glu Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Glu Arg Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Ile Ser 675 680 685 Pro Tyr Ser Cys Ile Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Glu Ala Ala Thr Pro Leu Lys Thr Met Ser 725 730 735 Ala Pro Ser Leu Asn Asn Thr Cys Ala Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Leu Gly Asn Pro Pro Ala Ala Thr Val Gln Asn Val Ser Ser Thr Ser 805 810 815 Ser Ser Asp Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ser Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Ser Arg Ser Ser Glu Pro Arg Gly Pro 885 890 895 Glu Thr Pro Glu Ala Lys Ser Leu Phe Asp Lys Val Ala Thr Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Val Asp Tyr Lys Pro Val Ser Ala Asn Gly Ala Glu Glu 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Thr Gln Lys Gln Asn Asp Gly Gln Lys Lys Asp Ser Ser Lys Asn 980 985 990 Gln Gly Ser Gly Leu Ser Ser Ser Gly Pro Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Lys Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu His Ala Ile Leu Glu Asp Ile 1395 1400 1405 His Val Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Arg Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Lys Val Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Phe Thr Ser Lys 1505 <210> 61 <211> 1495 <212> PRT <213> Artificial Sequence <220> <223> EPRS EFB20321.1 <400> 61 Val Gly Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile 1 5 10 15 Ser Val Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val 20 25 30 Val Phe Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala 35 40 45 Thr Thr Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile 50 55 60 Asp His Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu 65 70 75 80 Phe Thr Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr 85 90 95 Tyr Leu Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala 100 105 110 Thr Leu Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys 115 120 125 Ala Pro Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln 130 135 140 Ala Phe Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala 145 150 155 160 Lys Ala Ile Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu 165 170 175 Pro Gly Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala 180 185 190 Ser Gly Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln 195 200 205 His Tyr Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp 210 215 220 Thr Asn Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu 225 230 235 240 Asp Val Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser 245 250 255 Asp His Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu 260 265 270 Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu 275 280 285 Arg Glu Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Ile Glu Lys 290 295 300 Asn Leu Gln Met Trp Glu Glu Met Lys Gln Gly Ser Gln Phe Gly Gln 305 310 315 320 Ser Cys Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys 325 330 335 Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg 340 345 350 Thr Gly Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro 355 360 365 Ile Val Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu 370 375 380 Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly 385 390 395 400 Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn 405 410 415 Thr Val Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu 420 425 430 Val Asp Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu 435 440 445 Arg Arg Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln 450 455 460 Gly Ser Ser Arg Ser Ile Val Asn Met Glu Trp Asp Lys Ile Trp Ala 465 470 475 480 Phe Asn Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu 485 490 495 Leu Lys Lys Glu Val Ile Pro Val Asn Ile Pro Glu Ala Gln Glu Glu 500 505 510 Met Lys Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys 515 520 525 Pro Val Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu 530 535 540 Thr Leu Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile 545 550 555 560 Asn Ile Thr Lys Ile Asn Lys Asn Ala Asp Gly Lys Ile Val Ser Leu 565 570 575 Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys 580 585 590 Ile Thr Trp Leu Ala Glu Thr Ala His Ala Leu Pro Val Pro Ala Ile 595 600 605 Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp 610 615 620 Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu 625 630 635 640 Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile 645 650 655 Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro 660 665 670 Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile 675 680 685 Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Arg Glu Lys 690 695 700 Thr Lys Val Glu Thr Thr Lys Asn Glu Pro Thr Thr Pro Phe Lys Glu 705 710 715 720 Arg Pro Gly Pro Ser Leu Asp Asn Thr Cys Ala Pro Ser Glu Asp Ser 725 730 735 Leu Val Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu 740 745 750 Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp Val Asp Ala Ala Val Lys 755 760 765 Gln Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu 770 775 780 Tyr Lys Pro Gly Asn Pro Pro Ala Val Val Gln Asp Val Ser Ser Lys 785 790 795 800 Ser Ser Ala Ser Thr Val Asp Ser Lys Ser Leu Tyr Glu Glu Ile Ala 805 810 815 Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys 820 825 830 Ala Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln 835 840 845 Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu 850 855 860 Ser Gln Ser Ser Asp Ser Thr Asn Ser Ser Glu Pro Ser Gly Pro Glu 865 870 875 880 Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly Glu 885 890 895 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp 900 905 910 Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 915 920 925 Thr Gly Val Glu Tyr Lys Pro Val Ser Val Thr Gly Ala Glu Asp Lys 930 935 940 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 945 950 955 960 Pro Gln Lys Gln Ser Asp Gly Gln Lys Lys Asp Ser Phe Lys Ser Gln 965 970 975 Gly Ser Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys 980 985 990 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 995 1000 1005 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Leu Ile Glu Tyr Tyr 1010 1015 1020 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1025 1030 1035 1040 Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1045 1050 1055 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1060 1065 1070 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1075 1080 1085 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr 1090 1095 1100 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1105 1110 1115 1120 Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1125 1130 1135 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1140 1145 1150 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1155 1160 1165 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1170 1175 1180 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1185 1190 1195 1200 Gly Gly Asp Tyr Thr Thr Thr Leu Glu Val Phe Ile Ser Ala Ser Gly 1205 1210 1215 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1220 1225 1230 Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1235 1240 1245 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1250 1255 1260 Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1265 1270 1275 1280 Arg Val Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1285 1290 1295 Ala Leu Ser Glu Glu Asp Arg Glu Ala Leu Ile Ala Lys Cys Asn Asp 1300 1305 1310 Tyr Arg Lys Arg Leu Leu Ser Val Asn Ile Arg Val Arg Val Asp Leu 1315 1320 1325 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1330 1335 1340 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1345 1350 1355 1360 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala 1365 1370 1375 Asp Asn Glu Ala Glu Thr Lys Leu His Ala Leu Leu Glu Asp Ile His 1380 1385 1390 Val Asn Leu Phe Thr Arg Ala Ser Lys Asp Leu Lys Thr His Met Val 1395 1400 1405 Val Ala Asn Thr Met Glu Asp Phe Gln Gln Met Leu Asp Ser Gly Lys 1410 1415 1420 Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1425 1430 1435 1440 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1445 1450 1455 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1460 1465 1470 Gln Pro Gly Ala Lys Cys Ile Cys Gly Arg Asn Pro Ala Lys Tyr Tyr 1475 1480 1485 Thr Leu Phe Gly Arg Ser Tyr 1490 1495 <210> 62 <211> 1508 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004751515.1 <400> 62 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Ser Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Ser Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Leu Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Ala 165 170 175 Ile Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Met Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Asp Val Val Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile Asn Lys Ser Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala Arg Ala Leu Pro Ile Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Arg Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Glu Pro Ser Thr Pro Arg Lys Glu Arg Pro 725 730 735 Gly Pro Leu Asp Asn Thr Cys Ala Pro Ser Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Ala Val Val Gln Asn Val Ser Ser Gly Ser Ser Arg 805 810 815 Ser Val Val Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly 820 825 830 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Ile 835 840 845 Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu 850 855 860 Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln Ser 865 870 875 880 Ser Asp Ser Thr Asn Ser Ser Glu His Ser Ser Pro Glu Thr Pro Glu 885 890 895 Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg 900 905 910 Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Ser Ala Val 915 920 925 Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val 930 935 940 Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys 945 950 955 960 Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys 965 970 975 Gln Ser Asp Gly Gln Lys Lys Glu Ser Phe Lys Ser Gln Gly Ser Gly 980 985 990 Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr 995 1000 1005 Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr 1010 1015 1020 Ser Gln Val Ile Thr Lys Ser Glu Leu Ile Glu Tyr Tyr Asp Val Ser 1025 1030 1035 1040 Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp Glu Ser Ile 1045 1050 1055 Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys 1060 1065 1070 Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr 1075 1080 1085 His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly 1090 1095 1100 Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr Ser Glu Thr 1105 1110 1115 1120 Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu 1125 1130 1135 Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys 1140 1145 1150 His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly 1155 1160 1165 His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu Val Leu Gln 1170 1175 1180 Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro 1185 1190 1195 1200 Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp 1205 1210 1215 Tyr Thr Thr Thr Leu Glu Val Phe Ile Ser Ala Ser Gly Arg Ala Ile 1220 1225 1230 Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser Arg Met Phe 1235 1240 1245 Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala 1250 1255 1260 Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr 1265 1270 1275 1280 Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala 1285 1290 1295 Ser Val Gln Val Met Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser 1300 1305 1310 Glu Glu Asp Arg Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg 1315 1320 1325 Arg Leu Leu Ser Val Asn Ile Arg Val Arg Val Asp Leu Arg Asp Asn 1330 1335 1340 Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro 1345 1350 1355 1360 Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val 1365 1370 1375 Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala Asp Asn Glu 1380 1385 1390 Ala Glu Thr Lys Leu His Ala Leu Leu Glu Asp Ile His Val Asn Leu 1395 1400 1405 Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val Val Ala Asn 1410 1415 1420 Thr Met Glu Asp Phe Gln Lys Met Leu Asp Ser Gly Lys Ile Ala Gln 1425 1430 1435 1440 Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr 1445 1450 1455 Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala 1460 1465 1470 Lys Ser Leu Cys Ile Pro Phe Gln Pro Leu Cys Glu Leu Gln Pro Gly 1475 1480 1485 Ala Arg Cys Ile Cys Gly Arg Asn Pro Ala Lys Tyr Tyr Thr Leu Phe 1490 1495 1500 Gly Arg Ser Tyr 1505 <210> 63 <211> 1522 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_008683957.1 <400> 63 Met Glu Thr Ile Ser Gln Leu Ala Leu Leu Ser Leu Arg Ser Val Val 1 5 10 15 Lys Ser Ser Ser Leu Arg Arg Leu Pro Arg Arg Arg Glu Ser Pro Arg 20 25 30 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 35 40 45 Glu Xaa Asn Ile Leu His Val Ser Glu Ser Val Val Phe Thr Asp Ile 50 55 60 Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Ala Thr Ala Gly Leu 65 70 75 80 Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His Trp Leu Glu 85 90 95 Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr Ser Ala Ile 100 105 110 Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn 115 120 125 Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn 130 135 140 Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro Val His Val 145 150 155 160 Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe Gln Ser Val 165 170 175 Gly Thr Lys Trp Asn Val Ser Ala Ser Lys Ala Lys Ala Ile Pro Glu 180 185 190 Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met 195 200 205 Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His 210 215 220 Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn 225 230 235 240 Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys 245 250 255 Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu 260 265 270 His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr 275 280 285 Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val 290 295 300 Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Ile 305 310 315 320 Glu Ser Glu His Arg Lys Asn Ser Ile Glu Lys Asn Leu Gln Met Trp 325 330 335 Glu Glu Met Lys Gln Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu Arg 340 345 350 Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr 355 360 365 Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr 370 375 380 Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile 385 390 395 400 Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp 405 410 415 Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr 420 425 430 Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys 435 440 445 Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp 450 455 460 Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr 465 470 475 480 Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser 485 490 495 Ile Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Val 500 505 510 Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val 515 520 525 Ile Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys Glu Val Ala 530 535 540 Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val Trp Tyr Ser 545 550 555 560 Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu Ser Glu Gly 565 570 575 Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile 580 585 590 Asn Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala Lys Leu Asn 595 600 605 Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala 610 615 620 Glu Thr Ala His Ala Leu Pro Val Pro Ala Ile Cys Val Thr Tyr Glu 625 630 635 640 His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys Gln 645 650 655 Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro 660 665 670 Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg 675 680 685 Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser 690 695 700 Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp Gly His Thr 705 710 715 720 Lys Glu Met Pro Thr Ser Gly Ser Arg Glu Lys Thr Lys Val Glu Thr 725 730 735 Thr Lys Asn Glu Pro Thr Ala Pro Phe Lys Glu Arg Pro Gly Pro Ser 740 745 750 Leu Asp Asn Thr Cys Ala Pro Ser Glu Asp Ser Leu Val Leu Tyr Asn 755 760 765 Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys 770 775 780 Ala Ala Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu Leu Ala Leu 785 790 795 800 Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn 805 810 815 Pro Pro Ala Val Val Gln Asp Val Ser Ser Lys Ser Ser Ala Ser Thr 820 825 830 Val Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val 835 840 845 Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Val Asn Glu 850 855 860 Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr 865 870 875 880 Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp 885 890 895 Ser Thr Asn Ser Ser Glu Pro Ser Gly Pro Glu Thr Pro Glu Ala Lys 900 905 910 Val Leu Phe Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu 915 920 925 Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Thr Ala Val Gln Glu 930 935 940 Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val Glu Tyr 945 950 955 960 Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys 965 970 975 Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Ser 980 985 990 Asp Gly Gln Lys Lys Asp Ser Phe Lys Ser Gln Gly Ser Gly Leu Ser 995 1000 1005 Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu 1010 1015 1020 Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln 1025 1030 1035 1040 Val Ile Thr Lys Ser Glu Leu Ile Glu Tyr Tyr Asp Val Ser Gly Cys 1045 1050 1055 Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp Glu Ser Ile Lys Asp 1060 1065 1070 Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe 1075 1080 1085 Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Ile 1090 1095 1100 Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr 1105 1110 1115 1120 Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr Ser Glu Thr Val Met 1125 1130 1135 Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile 1140 1145 1150 Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro 1155 1160 1165 Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser 1170 1175 1180 Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu 1185 1190 1195 1200 Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val 1205 1210 1215 Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr 1220 1225 1230 Thr Thr Leu Glu Val Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly 1235 1240 1245 Ala Thr Ser His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile 1250 1255 1260 Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln 1265 1270 1275 1280 Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val 1285 1290 1295 His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Ser Val 1300 1305 1310 Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu 1315 1320 1325 Asp Arg Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Lys Arg Leu 1330 1335 1340 Leu Ser Val Ser Ile Arg Val Arg Val Asp Leu Arg Asp Asn Tyr Ser 1345 1350 1355 1360 Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg 1365 1370 1375 Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val 1380 1385 1390 Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala Asp Asn Glu Ala Glu 1395 1400 1405 Thr Lys Leu His Ser Leu Leu Glu Asp Ile His Val Asn Leu Phe Thr 1410 1415 1420 Arg Ala Ser Lys Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met 1425 1430 1435 1440 Glu Asp Phe Gln Gln Leu Leu Asp Ser Gly Lys Ile Val Gln Ile Pro 1445 1450 1455 Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala 1460 1465 1470 Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser 1475 1480 1485 Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys 1490 1495 1500 Cys Ile Cys Gly Arg Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg 1505 1510 1515 1520 Ser Tyr <210> 64 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_003999559.1 <400> 64 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Arg Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Pro Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Thr Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala His Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Thr Thr Lys Ala Lys Val 165 170 175 Thr Ser Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Xaa Ser 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Ile Lys Ile Asn Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr Gln Ala Leu Pro Val Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Ser Glu Pro Thr Ile Pro Phe Lys Glu Arg Pro 725 730 735 Gly Pro Ser Leu Asp Asn Thr Cys Ala Thr Phe Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Ala Thr Val Val Gln Asn Val Ser Ser Val Ser Val 805 810 815 Ala Ser Pro Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln 865 870 875 880 Ser Ser Asp Ser Ser Ala Thr Asn Ser Ser Glu Pro Arg Gly Pro Glu 885 890 895 Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Glu Gln Val Asp 915 920 925 Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Arg Ser Leu 930 935 940 Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys 945 950 955 960 Asp Lys Arg Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Gln Ser Asp Gly Pro Lys Lys Asp Ser Leu Lys Asn Gln 980 985 990 Gly Ser Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1250 1255 1260 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile Ala Lys Cys Asn Asp 1315 1320 1325 Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Val Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser His 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Phe Ala 1380 1385 1390 Asp Asn Glu Ala Glu Thr Lys Leu Gln Ala Val Leu Glu Asp Ile His 1395 1400 1405 Val Asn Leu Phe Thr Lys Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Met Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Thr Pro Leu Cys Glu Leu 1475 1480 1485 Gln Pro Gly Ala Arg Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 65 <211> 1571 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007072912.1 <400> 65 Met Thr His His Gly Val Arg Gly Asp Gly Gly Ser His Asp Ala Gly 1 5 10 15 Gly Gly Arg Arg Leu Arg Glu Arg Asp Gly Glu Ala Cys Arg Leu Pro 20 25 30 Glu Thr Val His Gly Val Ser Ser Ala Ala Leu Ile Phe Arg Pro Trp 35 40 45 Arg Val Asp Leu Leu Pro Glu Thr Val Tyr Ser Gly Val Glu Gly Gly 50 55 60 Val Phe Ile Lys Lys Asn Leu Phe Met Ser Leu Phe Leu Gly Ala Leu 65 70 75 80 Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val Glu Glu 85 90 95 Gly Asn Ile Leu His Val Ser Glu Asn Val Val Phe Thr Asp Ile Asn 100 105 110 Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr Ala Arg Leu Tyr 115 120 125 Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His Trp Leu Glu Phe 130 135 140 Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr Ser Ala Ile Asn 145 150 155 160 Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn Ser 165 170 175 Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn Pro 180 185 190 Ala Trp Gln Glu Gln Leu Lys Gln Ser Lys Thr Pro Val His Val Lys 195 200 205 Arg Trp Phe Gly Phe Leu Glu Ala His Gln Ala Phe Gln Ser Val Gly 210 215 220 Ala Lys Trp Asn Val Ser Thr Thr Lys Ala Lys Val Thr Ser Glu Lys 225 230 235 240 Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met Gly 245 250 255 Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His Ile 260 265 270 Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn Phe 275 280 285 Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys Glu 290 295 300 Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu His 305 310 315 320 Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile 325 330 335 Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp 340 345 350 Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Ile Glu 355 360 365 Ser Lys His Arg Lys Asn Ser Ile Glu Lys Asn Leu Gln Met Trp Glu 370 375 380 Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu Arg Ala 385 390 395 400 Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu 405 410 415 Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn 420 425 430 Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu 435 440 445 Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu 450 455 460 Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile 465 470 475 480 Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg 485 490 495 Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp 500 505 510 Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr Val 515 520 525 Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val 530 535 540 Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Val Ile 545 550 555 560 Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile 565 570 575 Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys 580 585 590 His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro 595 600 605 Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu Ser Glu Gly Glu 610 615 620 Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile Ile Lys Ile Asn 625 630 635 640 Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala Lys Leu Asn Leu 645 650 655 Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu 660 665 670 Thr Ala Gln Ala Leu Pro Ile Pro Ala Val Cys Val Thr Tyr Glu His 675 680 685 Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr 690 695 700 Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys 705 710 715 720 Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly 725 730 735 Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys 740 745 750 Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys 755 760 765 Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Val Glu Thr Thr 770 775 780 Lys Asn Glu Pro Thr Ile Pro Phe Lys Glu Arg Pro Gly Pro Ser Leu 785 790 795 800 Asp Asn Thr Cys Ala Thr Phe Glu Asp Ser Leu Val Leu Tyr Asn Arg 805 810 815 Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala 820 825 830 Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu Ala Leu Lys 835 840 845 Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro 850 855 860 Ala Ala Leu Val Gln Asn Val Ser Ser Val Ser Val Ala Arg Pro Leu 865 870 875 880 Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val 885 890 895 Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Val Asn Glu Ala 900 905 910 Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly 915 920 925 Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser Gln Ser Ser Asp Ser 930 935 940 Ser Ala Thr Asn Ser Ser Glu Pro Arg Gly Pro Glu Thr Pro Glu Ala 945 950 955 960 Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys 965 970 975 Leu Lys Ala Glu Lys Ala Ser Lys Glu Gln Val Asp Thr Ala Val Gln 980 985 990 Glu Leu Leu Arg Leu Lys Ala Gln Tyr Thr Ser Leu Thr Gly Val Glu 995 1000 1005 Tyr Gln Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Arg Lys 1010 1015 1020 Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln 1025 1030 1035 1040 Ser Asp Gly Pro Lys Lys Asp Ser Leu Lys Asn Arg Gly Ser Gly Leu 1045 1050 1055 Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Arg Gln Thr Arg 1060 1065 1070 Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser 1075 1080 1085 Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly 1090 1095 1100 Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp Glu Ser Ile Lys 1105 1110 1115 1120 Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr 1125 1130 1135 Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His 1140 1145 1150 Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys 1155 1160 1165 Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr Ser Glu Thr Val 1170 1175 1180 Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro 1185 1190 1195 1200 Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His 1205 1210 1215 Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His 1220 1225 1230 Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu Val Leu Gln Ile 1235 1240 1245 Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val 1250 1255 1260 Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr 1265 1270 1275 1280 Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln 1285 1290 1295 Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu 1300 1305 1310 Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr 1315 1320 1325 Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met 1330 1335 1340 Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys 1345 1350 1355 1360 Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu 1365 1370 1375 Glu Asp Arg Asp Ala Leu Leu Ala Lys Cys Asn Asp Tyr Arg Arg Arg 1380 1385 1390 Leu Leu Ser Val Asn Ile Arg Val Arg Val Asp Leu Arg Asp Asn Tyr 1395 1400 1405 Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile 1410 1415 1420 Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Arg Gln Phe Val Ala 1425 1430 1435 1440 Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Phe Ala Asp Asn Glu Ala 1445 1450 1455 Glu Thr Lys Leu Gln Ala Val Leu Glu Asp Ile His Val Asn Leu Phe 1460 1465 1470 Thr Lys Ala Ser Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr 1475 1480 1485 Met Glu Asp Phe Gln Lys Met Leu Asp Ser Gly Lys Ile Ala Gln Ile 1490 1495 1500 Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr 1505 1510 1515 1520 Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys 1525 1530 1535 Ser Leu Cys Ile Pro Phe Thr Pro Leu Cys Glu Leu Gln Pro Gly Ala 1540 1545 1550 Arg Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly 1555 1560 1565 Arg Ser Tyr 1570 <210> 66 <211> 1438 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_008586040.1 <400> 66 Met Glu His Thr Glu Ile Asp His Trp Leu Glu Phe Ser Ala Thr Lys 1 5 10 15 Leu Ser Ser Cys Ser Leu Phe Thr Ser Ala Ile Asn Glu Leu Asn His 20 25 30 Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn Met Leu Ser Leu Ala 35 40 45 Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn Ala Ala Trp Gln Glu 50 55 60 Gln Leu Lys Gln Asn Lys Ser Leu Val His Ala Lys Arg Trp Phe Gly 65 70 75 80 Phe Leu Glu Ala Gln Gln Thr Phe Gln Ser Val Gly Thr Lys Trp Asp 85 90 95 Val Ser Thr Thr Thr Thr Arg Val Ala Pro Glu Lys Lys Gln Asp Val 100 105 110 Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met Gly Lys Val Thr Val 115 120 125 Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His Ile Gly His Ala Lys 130 135 140 Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn Phe Lys Gly Lys Leu 145 150 155 160 Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys Glu Lys Glu Asp Phe 165 170 175 Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu His Ile Lys Pro Asp 180 185 190 Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile Met Lys Tyr Ala 195 200 205 Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala 210 215 220 Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Ile Glu Ser Lys His Arg 225 230 235 240 Lys Asn Ser Val Glu Lys Asn Leu Gln Met Trp Glu Glu Met Lys Lys 245 250 255 Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile Asp Met 260 265 270 Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys 275 280 285 Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr Pro Thr 290 295 300 Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val Thr His 305 310 315 320 Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp 325 330 335 Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser 340 345 350 Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu Thr Trp 355 360 365 Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg Phe Pro 370 375 380 Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly Leu Lys 385 390 395 400 Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn Met Glu 405 410 415 Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Val Ile Asp Pro Val Ala 420 425 430 Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Ile 435 440 445 Pro Glu Ala Gln Glu Ala Met Lys Glu Val Ala Arg His Pro Lys Asn 450 455 460 Pro Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Ile 465 470 475 480 Glu Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe 485 490 495 Ile Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Asn Ala Asp 500 505 510 Gly Lys Ile Val Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp 515 520 525 Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr His Ala 530 535 540 Leu Pro Ile Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys 545 550 555 560 Pro Val Leu Gly Lys Glu Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn 565 570 575 Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu 580 585 590 Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys 595 600 605 Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Arg Glu Ala Pro 610 615 620 Cys Ile Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Met 625 630 635 640 Phe Gly Ser Lys Glu Lys Thr Lys Val Glu Ala Thr Lys Asn Glu Thr 645 650 655 Thr Thr Pro Leu Lys Glu Lys Pro Ala Ala Ser Leu Asn Asn Thr Cys 660 665 670 Gly Thr Phe Glu Ser Val Val Leu Tyr Asn Arg Val Ala Val Gln Gly 675 680 685 Asp Met Val Arg Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp Ile 690 695 700 Asp Ala Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu 705 710 715 720 Lys Thr Gly Gln Glu Tyr Lys Pro Gly His Pro Pro Ala Val Val Gln 725 730 735 Asp Ile Ser Ser Lys Ser Ser Ala Ser Ile Leu Glu Ser Gln Ser Leu 740 745 750 Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala 755 760 765 Asp Lys Ala Pro Lys Ala Lys Ile Asn Glu Ala Val Glu Cys Leu Leu 770 775 780 Ser Leu Lys Thr Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro 785 790 795 800 Gly Gln Pro Pro Leu Ser Arg Ser Ser Asp Ser Ser Pro Thr Arg Asn 805 810 815 Ser Glu Ser Thr Gly Ser Glu Thr Pro Glu Ala Lys Val Leu Phe Asp 820 825 830 Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys 835 840 845 Ala Ser Lys Asp Gln Ile Asp Val Ala Val Gln Glu Leu Leu Gln Leu 850 855 860 Lys Ala Gln Tyr Thr Ser Leu Thr Gly Val Glu Tyr Lys Pro Val Ser 865 870 875 880 Val Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn 885 890 895 Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Gln Arg 900 905 910 Lys Asp Pro Ser Lys Asn Gln Gly Ser Gly Leu Ser Pro Ser Gly Ala 915 920 925 Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala 930 935 940 Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys 945 950 955 960 Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg 965 970 975 Pro Trp Ala Tyr Ser Ile Trp Glu Phe Ile Lys Asp Phe Phe Asp Ala 980 985 990 Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe Val 995 1000 1005 Ser Gln Ser Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe Ala 1010 1015 1020 Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu 1025 1030 1035 1040 Pro Ile Ala Val Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr 1045 1050 1055 Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln 1060 1065 1070 Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu 1075 1080 1085 Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr 1090 1095 1100 Phe Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala 1105 1110 1115 1120 Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys 1125 1130 1135 Thr Glu Lys Glu Lys Phe Ala Gly Gly His Tyr Thr Thr Thr Val Glu 1140 1145 1150 Ala Phe Ile Ser Ala Ser Gly Arg Ala Val Gln Gly Ala Thr Ser His 1155 1160 1165 His Leu Gly Gln Asn Phe Ser Arg Met Phe Glu Ile Ile Phe Glu Asp 1170 1175 1180 Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly 1185 1190 1195 1200 Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp Asn 1205 1210 1215 Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val Val 1220 1225 1230 Ile Pro Cys Gly Ile Thr Asn Ala Leu Pro Glu Glu Asp Arg Glu Ala 1235 1240 1245 Leu Ile Thr Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn 1250 1255 1260 Ile Arg Val Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys 1265 1270 1275 1280 Phe Asn His Trp Glu Leu Lys Gly Val Pro Val Arg Leu Glu Val Gly 1285 1290 1295 Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr 1300 1305 1310 Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln 1315 1320 1325 Ala Ile Leu Glu Asp Ile His Val Asn Leu Phe Thr Arg Ala Ser Glu 1330 1335 1340 Asp Leu Lys Thr His Met Val Ile Ala Asp Thr Met Glu Asp Phe Gln 1345 1350 1355 1360 Lys Ala Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu 1365 1370 1375 Val Asp Cys Glu Asp Trp Ile Lys Lys Ala Thr Ala Arg Asp Gln Asp 1380 1385 1390 Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro 1395 1400 1405 Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Arg Cys Val Cys Gly 1410 1415 1420 Lys Asn Val Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1425 1430 1435 <210> 67 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006188215.1 <400> 67 Met Gly Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Ser Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Thr Ala Trp Gln Asp Gln Leu Glu Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Thr Thr Lys Ala Lys Ala 165 170 175 Ala Pro Glu Lys Lys Gln Asp Ile Gly Arg Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Ile Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Glu Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Leu Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Lys Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Leu Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala Arg Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asp Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Val Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Gln Thr Pro Ser Pro Leu Lys Thr Arg Ser 725 730 735 Pro Pro Ser Leu Asn Ser Thr Cys Ala Thr Ser Glu Glu Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Asp Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Leu Val Val Gln Asp Val Ser Ser Lys Ser 805 810 815 Ser Ser Gly Ile Leu Asp Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Ser Pro 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Thr Arg Ser Ala Glu Pro Ser Gly Pro 885 890 895 Glu Thr Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala Leu Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Val Asp Tyr Lys Pro Val Ser Thr Ile Gly Ala Glu Glu 945 950 955 960 Lys Asp Lys Arg Lys Lys Glu Lys Glu Asn Lys Cys Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Ser Leu Lys Lys Asp Trp Ser Lys Ser 980 985 990 Gln Gly Ser Gly Leu Pro Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Ile Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Ile Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Lys Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Arg Ala Val Leu Glu Asp Ile 1395 1400 1405 His Val Thr Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 68 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006198933.1 <400> 68 Met Gly Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Ser Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Thr Ala Trp Gln Asp Gln Leu Glu Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Thr Lys Trp Asn Val Ser Thr Thr Lys Ala Lys Ala 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Ile Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Glu Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Leu Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Leu Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala Arg Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asp Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Val Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Gln Thr Pro Ser Pro Leu Lys Thr Arg Ser 725 730 735 Pro Pro Ser Leu Asn Ser Thr Cys Ala Thr Ser Glu Glu Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Asp Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Leu Val Val Gln Asp Val Ser Ser Lys Ser 805 810 815 Ser Ser Gly Ile Leu Asp Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Ser Pro 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Thr Arg Ser Ala Glu Pro Ser Gly Pro 885 890 895 Glu Thr Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala Leu Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Val Asp Tyr Lys Pro Val Ser Ala Ile Gly Ala Glu Glu 945 950 955 960 Lys Asp Lys Arg Lys Lys Glu Lys Glu Asn Lys Cys Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Ser Leu Lys Lys Asp Trp Ser Lys Ser 980 985 990 Gln Gly Ser Gly Leu Pro Ser Asn Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Thr Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Val Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Thr Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Lys Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Arg Ala Ile Leu Glu Asp Ile 1395 1400 1405 His Val Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 69 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004480290.1 <400> 69 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Thr Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Asp Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Val Phe Thr 85 90 95 Ser Ala Ile Asn Asp Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Thr Leu Ser Leu Ala Asp Ile Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu His Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Pro Thr Asn Lys Ala Lys Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Gly Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys Gln Arg Asn Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile His Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro His Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Ile Gly Leu Lys Leu Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Arg Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Ala Glu Pro Thr Lys Asn Glu Thr Ala Ala Pro Phe Asn Gly Ser Ser 725 730 735 Ala Pro Ser Leu Ser Asn Thr Cys Ala Ala Ala Glu Asp Ser Leu Ala 740 745 750 Leu Tyr Ser Lys Val Ala Ala Gln Gly Asp Val Val Arg Asp Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Gly Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Val Val Val Gln Asn Ile Pro Ser Lys Ser 805 810 815 Ser Ala Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Arg Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Asn Ser Asp Ser Gly Pro Thr Arg Ser Ala Glu Pro Ser Gly Pro 885 890 895 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Ser Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Ile 915 920 925 Asp Leu Ala Val Gln Glu Leu Leu Gly Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Val Glu Tyr Lys Pro Leu Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Pro Asn Asp Gly Gln Lys Lys Asp Ser Ser Lys Ser 980 985 990 Gln Gly Gly Gly Leu Ala Pro Gly Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Val Ser Ala Asn 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Ala Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Ser Thr Arg Thr Leu 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Lys Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Asn 1365 1370 1375 Cys Gln Phe Ile Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Met Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Leu Leu Glu Asp Ile 1395 1400 1405 His Val Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr Arg Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Glu Phe Gln Lys Ala Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Arg Cys Leu Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 70 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_008266633.1 <400> 70 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Leu Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Val Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Asn Arg 50 55 60 Val Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Thr Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Ser Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Ser Lys Trp Gly Val Ser Ala Asn Lys Ala Lys Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Leu Glu Ser Lys His Arg Gln Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Thr Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Ser Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Ser Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ser Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Ile Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys Arg Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro His Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Ile Lys Asn Glu Thr Thr Ala Pro Phe Lys Asp Arg Pro 725 730 735 Ala Pro Ser Leu Ser Asn Thr Cys Ala Thr Ser Glu Asp Pro Val Leu 740 745 750 Leu Tyr Asn Lys Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ser Lys Lys Ala Ala Lys Glu Asp Ile Asp Ile Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Thr Ala Val Ile Gln Asn Ile Ser Ser Lys Ser 805 810 815 Ser Thr Ser Val Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr 850 855 860 Lys Ala Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Ala Gly Asn Ser Asp Pro Arg Gly Pro 885 890 895 Glu Thr Pro Glu Ala Lys Ala Leu Phe Asp Lys Val Ala Cys Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Thr Ser Lys Asp Gln Ile 915 920 925 Asp Ala Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Glu 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Ser Gln Arg Lys Asp Thr Ser Lys Ser 980 985 990 Gln Gly Gly Gly Leu Ser Ala Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ser Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Arg Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Ile Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Cys Arg Arg Leu Leu Ser Val Ser Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Asn 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Thr Ile Leu Glu Asp Ile 1395 1400 1405 Gln Ala Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asp Thr Met Glu Asp Phe Gln Lys Ile Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Arg Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Ile Cys Gly Lys Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 71 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006056008.1 <400> 71 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ala Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Gly Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Thr Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Ser Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Glu Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Phe Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Ser Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Ser Ala Glu Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Glu His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Ile Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Ala Lys Asn Glu Ala Ser Thr Pro Leu Lys Thr Arg Ser 725 730 735 Ala Pro Ser Leu Ser Thr Cys Ala Thr Ser Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Thr Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Ala Ala Val Gly Gln Asn Ile Ser Ser Lys Ser Ser 805 810 815 Ser Asn Ile Val Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Leu Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ser Ser Gln 865 870 875 880 Ser Ser Asp Ser Ser Pro Pro Arg Ser Ser Glu Pro Ser Gly Pro Glu 885 890 895 Thr Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala Ser Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp 915 920 925 Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Thr Gly Val Asp Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Glu Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Gln Asn Asp Ser Gln Lys Lys Asp Ser Ser Lys Asn Gln 980 985 990 Glu Ser Gly Leu Ser Pro Asn Gly Thr Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Arg Lys Glu Asp Ser Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Lys Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Val Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Asp Leu Tyr Ala Arg Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Ala Gly Glu Lys 1250 1255 1260 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Thr Met Val His Gly Asp Asn Val Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Leu Ala Lys Cys Asn Asp 1315 1320 1325 Tyr Arg Arg Arg Leu Leu Ser Val Ser Ile Arg Val Arg Val Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Asn Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala 1380 1385 1390 Glu Asn Glu Ala Glu Thr Lys Leu His Ala Ile Leu Glu Asp Ile His 1395 1400 1405 Ile Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1475 1480 1485 Pro Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 72 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007935118.1 <400> 72 Met Glu Thr Leu Cys Leu Thr Val Asn Ala Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Val Ala Val Glu His Val Lys Asp Ser Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Ser Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr 85 90 95 Ser Ala Ile Ser Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly His Ala Thr Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Leu 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Ser Lys Trp Tyr Val Ser Thr Thr Lys Pro Lys Val 165 170 175 Ala Ser Glu Lys Lys Gln Asp Val Gly Lys Phe Ile Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Val Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Asn Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Thr Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Thr Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Thr Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Ile Pro Glu Ala Gln Glu Lys Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Arg Asn Thr Asp Gly Lys Ile Leu Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Phe Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Arg Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Met Ala Lys Asn Glu Thr Ile Pro Cys Lys Glu Lys Pro Ala 725 730 735 Pro Ala Met Asp Asn Thr Cys Ala Thr Ser Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Val Gln Gly Glu Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Ala Lys Glu Asp Ile Asp Ile Ala Val Lys Gln Leu Leu 770 775 780 Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Gln Pro 785 790 795 800 Gly Asn Pro Pro Ala Ala Val Val Gln Asn Ile Ser Ser Glu Ser Ser 805 810 815 Glu Gly Ile Leu Asp Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Glu Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Val Pro Arg Gln Pro Leu Leu Ser Gln 865 870 875 880 Asn Ser Asp Ser Ser Pro Thr Arg Ser Ser Glu His Ser Gly Pro Glu 885 890 895 Thr Pro Glu Ala Lys Gly Leu Phe Asp Lys Val Ala Ser Gln Gly Glu 900 905 910 Val Val Arg Thr Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Ile Glu 915 920 925 Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Thr Gly Val Glu Tyr Lys Pro Val Ser Val Thr Gly Ala Glu Asp Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Ser Gln Lys Gln Asn Asp Gly Gln Arg Lys Asp Ser Ser Lys Asn Gln 980 985 990 Gly Ser Gly Gln Ser Ser Gly Gly Pro Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1250 1255 1260 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Glu Lys Cys Asn Asp 1315 1320 1325 Tyr Arg Lys Arg Leu Leu Asn Val Asn Ile Arg Val Arg Val Asp Leu 1330 1335 1340 Arg Glu Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Thr 1380 1385 1390 Glu Asn Glu Ala Glu Ser Lys Leu Gln Ala Ile Leu Glu Asp Ile His 1395 1400 1405 Val Asn Leu Tyr Thr Arg Ala Ser Gln Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asn Thr Met Asp Asp Phe Gln Lys Val Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Thr Pro Phe Lys Pro Leu Cys Glu Leu 1475 1480 1485 Gln Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 73 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007455449.1 <400> 73 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Xaa Xaa Xaa Xaa Xaa Xaa Gly Lys Glu Asn Ile Leu His 35 40 45 Val Ser Glu Asn Val Val Phe Thr Asp Ile Asn Ser Ile Leu Arg Tyr 50 55 60 Leu Ala Arg Ile Ala Thr Thr Ala Gly Leu Tyr Gly Ser Asn Leu Leu 65 70 75 80 Glu His Thr Glu Ile Asp His Trp Leu Glu Phe Ser Ala Thr Lys Leu 85 90 95 Ser Ser Cys Asp Leu Phe Thr Ser Ala Ile Asn Glu Leu Ser His Cys 100 105 110 Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn Ser Leu Ser Leu Ala Asp 115 120 125 Leu Cys Val Trp Ala Thr Leu Lys Gly Asn Ala Ala Trp Gln Glu Gln 130 135 140 Leu Glu Gln Asn Lys Ala Pro Val His Val Lys Arg Trp Phe Gly Phe 145 150 155 160 Leu Glu Ala Gln Gln Ala Phe Gln Ser Val Gly Thr Lys Trp Asn Val 165 170 175 Ser Thr Thr Lys Ala Lys Val Val Pro Glu Lys Lys Gln Asp Val Gly 180 185 190 Lys Phe Val Glu Leu Pro Gly Ala Glu Met Gly Lys Val Thr Val Arg 195 200 205 Phe Pro Pro Glu Ala Ser Gly Tyr Leu His Ile Gly His Ala Lys Ala 210 215 220 Ala Leu Leu Asn Gln His Tyr Gln Val Asn Phe Lys Gly Lys Leu Ile 225 230 235 240 Met Arg Phe Asp Asp Thr Asn Pro Glu Lys Glu Lys Glu Asp Phe Glu 245 250 255 Lys Val Ile Leu Glu Asp Val Ala Met Leu His Ile Lys Pro Asp Gln 260 265 270 Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile Met Lys Tyr Ala Glu 275 280 285 Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala Glu 290 295 300 Gln Met Lys Ala Glu Arg Glu Gln Arg Ile Glu Ser Lys His Arg Lys 305 310 315 320 Asn Ser Val Glu Lys Asn Leu Gln Met Trp Glu Glu Met Lys Lys Gly 325 330 335 Ser Gln Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile Asp Met Ser 340 345 350 Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys Ile 355 360 365 Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr Pro Thr Tyr 370 375 380 Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val Thr His Ala 385 390 395 400 Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp Ile 405 410 415 Ile Glu Ala Leu Gly Leu Arg Lys Pro Tyr Ile Trp Glu Tyr Ser Arg 420 425 430 Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu Thr Trp Phe 435 440 445 Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg Phe Pro Thr 450 455 460 Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly Leu Lys Gln 465 470 475 480 Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn Met Glu Trp 485 490 495 Asp Lys Ile Trp Ala Phe Asn Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asp Ile Pro 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Arg His Pro Lys Asn Pro 530 535 540 Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Ile Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Leu Ser Glu Gly Glu Thr Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile His Arg Asn Ala Glu Gly 580 585 590 Lys Ile Val Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Glu Arg Ala Leu 610 615 620 Pro Val Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Ile Ser Pro Tyr Ser Cys Ile Glu Ala Pro Cys 690 695 700 Ile Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Thr Thr Lys Asn Glu Ala Ala 725 730 735 Thr Pro Leu Lys Thr Val Ser Ala Pro Ser Leu Asn Asn Thr Cys Ala 740 745 750 Thr Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Val Gln Gly 755 760 765 Asn Val Val Arg Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp Ile 770 775 780 Asp Ala Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Lys Leu Gly Asn Pro Pro Ala Ala Ile Val 805 810 815 Gln Asn Val Ser Ser Thr Ser Ser Ser Asp Ile Leu Glu Arg Lys Ser 820 825 830 Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys 835 840 845 Ala Glu Lys Ala Pro Lys Ala Lys Val Asn Glu Ala Val Glu Cys Leu 850 855 860 Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Val 865 870 875 880 Pro Gly Gln Pro Pro Ser Ser Gln Ser Ser Asp Ser Ser Pro Ser Arg 885 890 895 Ser Ser Glu Leu His Gly Pro Glu Thr Pro Glu Ala Lys Ser Leu Phe 900 905 910 Asp Arg Val Ala Thr Gln Gly Asp Val Val Arg Lys Leu Lys Ala Glu 915 920 925 Lys Ala Ser Lys Asp Gln Val Asp Thr Ala Val Gln Glu Leu Leu Gln 930 935 940 Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val Asp Tyr Lys Pro Val 945 950 955 960 Ser Ala Asn Gly Ala Glu Glu Lys Asp Lys Lys Lys Lys Glu Lys Glu 965 970 975 Asn Lys Ser Glu Lys Gln Asn Lys Thr Gln Lys Gln Asn Asp Gly Gln 980 985 990 Lys Lys Asp Ser Cys Lys Asn Gln Gly Ser Gly Leu Ser Ser Ser Gly 995 1000 1005 Pro Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Ala Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp 1060 1065 1070 Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe 1075 1080 1085 Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Val Ala Asp Phe 1090 1095 1100 Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala 1105 1110 1115 1120 Glu Pro Ile Ala Val Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala 1125 1130 1135 Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn 1140 1145 1150 Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe 1155 1160 1165 Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala 1170 1175 1180 Thr Phe Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr 1185 1190 1195 1200 Ala Lys Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg 1205 1210 1215 Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val 1220 1225 1230 Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser 1235 1240 1245 His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Ile Phe Glu 1250 1255 1260 Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp 1265 1270 1275 1280 Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp 1285 1290 1295 Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val 1300 1305 1310 Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg Asp 1315 1320 1325 Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser Val 1330 1335 1340 Asn Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp 1345 1350 1355 1360 Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val 1365 1370 1375 Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp 1380 1385 1390 Thr Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys Leu 1395 1400 1405 His Ala Ile Leu Glu Asp Ile His Val Asn Leu Phe Thr Arg Ala Ser 1410 1415 1420 Glu Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp Phe 1425 1430 1435 1440 Gln Lys Glu Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln 1460 1465 1470 Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Arg Pro Leu Cys Glu Leu Gln Pro Gly Ala Gln Cys Ile Cys 1490 1495 1500 Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 74 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS NP_001230249.1 <400> 74 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Ala 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Gly Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Glu Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Lys Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Lys Ala His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Phe Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Ser Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Ser Ala Glu Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Glu His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Ile Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Glu Ala Ser Thr Pro Leu Lys Thr Arg Ser 725 730 735 Ala Pro Ser Leu Ser Thr Cys Ala Thr Ser Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Thr Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Ala Ala Val Gly Gln Asn Ile Ser Ser Lys Ser Ser 805 810 815 Ser Asn Ile Val Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ser Ser Gln 865 870 875 880 Ser Ser Asp Ser Ser Pro Pro Arg Ser Ser Glu Pro Ser Gly Pro Glu 885 890 895 Thr Pro Glu Ala Lys Leu Leu Phe Asp Lys Val Ala Ser Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp 915 920 925 Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Ala Gly Val Asp Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Glu Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Gln Asn Asp Ser Gln Lys Lys Asp Ser Phe Lys Thr Gln 980 985 990 Gly Ser Gly Leu Ser Pro Asn Gly Ala Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Arg Lys Glu Asp Ser Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Val Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Asp Leu Tyr Ala Arg Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Ala Gly Glu Lys 1250 1255 1260 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Thr Met Val His Gly Asp Asn Val Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Leu Ala Lys Cys Ser Asp 1315 1320 1325 Tyr Gln Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Val Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Asn Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala 1380 1385 1390 Glu Asn Glu Ala Glu Thr Lys Leu His Ala Ile Leu Glu Asp Ile His 1395 1400 1405 Ile Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1475 1480 1485 Pro Pro Gly Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 75 <211> 1518 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006056007.1 <400> 75 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ala Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Gly Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Thr Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Ser Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Glu Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Phe Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Val Pro Val Asn Ile Pro 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Pro 530 535 540 Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Ser Lys Val Phe Ile Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Leu Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Ser Ala Glu Gly 580 585 590 Lys Ile Val Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Glu His Ala Leu 610 615 620 Pro Val Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Ile Glu Ala Pro Cys 690 695 700 Ile Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Thr Ala Lys Asn Glu Ala Ser 725 730 735 Thr Pro Leu Lys Thr Arg Ser Ala Pro Ser Leu Ser Thr Cys Ala Thr 740 745 750 Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp 755 760 765 Val Val Arg Glu Leu Lys Ala Lys Lys Ala Thr Lys Glu Asp Ile Asp 770 775 780 Ala Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys 785 790 795 800 Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Ala Val Gly Gln 805 810 815 Asn Ile Ser Ser Lys Ser Ser Ser Asn Ile Val Glu Ser Lys Ser Leu 820 825 830 Tyr Asp Glu Val Ala Ala Gln Gly Glu Leu Val Arg Lys Leu Lys Ala 835 840 845 Glu Lys Ala Pro Lys Ala Lys Val Asn Glu Ala Val Glu Cys Leu Leu 850 855 860 Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro 865 870 875 880 Gly Gln Pro Pro Ser Ser Gln Ser Ser Asp Ser Ser Pro Pro Arg Ser 885 890 895 Ser Glu Pro Ser Gly Pro Glu Thr Pro Glu Ala Lys Leu Leu Phe Asp 900 905 910 Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys 915 920 925 Ala Ser Lys Asp Gln Val Asp Thr Ala Val Gln Glu Leu Leu Gln Leu 930 935 940 Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val Asp Tyr Lys Pro Val Ser 945 950 955 960 Ala Thr Gly Ala Glu Glu Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn 965 970 975 Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Ser Gln Lys 980 985 990 Lys Asp Ser Ser Lys Asn Gln Glu Ser Gly Leu Ser Pro Asn Gly Thr 995 1000 1005 Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala 1010 1015 1020 Arg Lys Glu Asp Ser Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys 1025 1030 1035 1040 Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg 1045 1050 1055 Pro Trp Ala Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala 1060 1065 1070 Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe Val 1075 1080 1085 Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe Ala 1090 1095 1100 Pro Glu Val Ala Trp Val Thr Lys Ser Gly Lys Thr Glu Leu Ala Glu 1105 1110 1115 1120 Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr 1125 1130 1135 Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Val Lys Leu Asn Gln 1140 1145 1150 Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu 1155 1160 1165 Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr 1170 1175 1180 Phe Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala 1185 1190 1195 1200 Arg Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys 1205 1210 1215 Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu 1220 1225 1230 Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His 1235 1240 1245 His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Ile Phe Glu Asp 1250 1255 1260 Pro Lys Thr Ala Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly 1265 1270 1275 1280 Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp Asn 1285 1290 1295 Val Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val Val 1300 1305 1310 Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala 1315 1320 1325 Leu Leu Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser Val Ser 1330 1335 1340 Ile Arg Val Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys 1345 1350 1355 1360 Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly 1365 1370 1375 Pro Arg Asp Met Lys Asn Cys Gln Phe Val Ala Val Arg Arg Asp Thr 1380 1385 1390 Gly Glu Lys Leu Thr Ile Ala Glu Asn Glu Ala Glu Thr Lys Leu His 1395 1400 1405 Ala Ile Leu Glu Asp Ile His Ile Asn Leu Phe Thr Arg Ala Ser Glu 1410 1415 1420 Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp Phe Gln 1425 1430 1435 1440 Lys Glu Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu 1445 1450 1455 Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp 1460 1465 1470 Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro 1475 1480 1485 Phe Lys Pro Leu Cys Glu Leu Pro Pro Gly Ala Lys Cys Val Cys Gly 1490 1495 1500 Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 76 <211> 1508 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005905625.1 <400> 76 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Ile Leu His Val Ser Glu Asn Val Val Phe Thr Asp Ile 35 40 45 Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Ala Ala Gly Leu 50 55 60 Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His Trp Leu Glu 65 70 75 80 Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr Ser Ala Val 85 90 95 Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn 100 105 110 Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn 115 120 125 Ala Ala Trp Gln Glu Gln Leu Gly Gln Asn Lys Ala Pro Val His Val 130 135 140 Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe Gln Ser Val 145 150 155 160 Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Val Val Pro Glu 165 170 175 Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met 180 185 190 Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His 195 200 205 Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn 210 215 220 Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys 225 230 235 240 Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu 245 250 255 His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr 260 265 270 Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val 275 280 285 Asp Asp Thr Pro Ala Glu Gln Met Lys Val Glu Arg Glu Gln Arg Ile 290 295 300 Glu Ser Lys His Arg Glu Asn Ser Val Glu Lys Asn Leu Gln Met Trp 305 310 315 320 Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu Arg 325 330 335 Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr 340 345 350 Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr 355 360 365 Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile 370 375 380 Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp 385 390 395 400 Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Phe 405 410 415 Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys 420 425 430 Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp 435 440 445 Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr 450 455 460 Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser 465 470 475 480 Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Val 485 490 495 Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val 500 505 510 Val Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys Glu Val Ala 515 520 525 Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val Trp Tyr Ser 530 535 540 Ser Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu Ser Glu Gly 545 550 555 560 Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile 565 570 575 His Lys Ser Ala Glu Gly Lys Ile Val Ser Leu Asp Ala Lys Leu Asn 580 585 590 Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala 595 600 605 Glu Thr Glu His Ala Leu Pro Val Pro Ala Ile Cys Val Thr Tyr Glu 610 615 620 His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys Gln 625 630 635 640 Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro 645 650 655 Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg 660 665 670 Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser 675 680 685 Cys Ile Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp Gly His Thr 690 695 700 Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Val Glu Thr 705 710 715 720 Thr Lys Asn Glu Ala Ser Thr Pro Leu Lys Thr Arg Ser Ala Pro Ser 725 730 735 Leu Ser Thr Cys Ala Thr Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg 740 745 750 Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala 755 760 765 Thr Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu Ala Leu Lys 770 775 780 Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro 785 790 795 800 Pro Ala Ala Val Gly Gln Asn Ile Ser Ser Lys Ser Ser Ser Asn Ile 805 810 815 Val Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val 820 825 830 Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Val Asn Glu 835 840 845 Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr 850 855 860 Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ser Ser Gln Ser Ser Asp 865 870 875 880 Ser Ser Pro Pro Arg Ser Ser Glu Pro Ser Gly Pro Glu Thr Pro Glu 885 890 895 Ala Lys Leu Leu Phe Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg 900 905 910 Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Thr Ala Val 915 920 925 Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val 930 935 940 Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Glu Lys Asp Lys Lys 945 950 955 960 Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys 965 970 975 Gln Asn Asp Ser Gln Lys Lys Asp Ser Phe Lys Thr Gln Gly Ser Gly 980 985 990 Leu Ser Pro Asn Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr 995 1000 1005 Arg Leu Gly Leu Glu Ala Arg Lys Glu Asp Ser Leu Ala Asp Trp Tyr 1010 1015 1020 Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser 1025 1030 1035 1040 Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp Glu Ser Ile 1045 1050 1055 Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys 1060 1065 1070 Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr 1075 1080 1085 His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly 1090 1095 1100 Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr 1105 1110 1115 1120 Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu 1125 1130 1135 Pro Val Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys 1140 1145 1150 His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly 1155 1160 1165 His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu Val Leu Gln 1170 1175 1180 Ile Leu Asp Leu Tyr Ala Arg Val Tyr Glu Glu Leu Leu Ala Ile Pro 1185 1190 1195 1200 Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp 1205 1210 1215 Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile 1220 1225 1230 Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser Lys Met Phe 1235 1240 1245 Glu Ile Val Phe Glu Asp Pro Lys Thr Ala Gly Glu Lys Gln Phe Ala 1250 1255 1260 Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr 1265 1270 1275 1280 Met Val His Gly Asp Asn Val Gly Leu Val Leu Pro Pro Arg Val Ala 1285 1290 1295 Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser 1300 1305 1310 Glu Glu Asp Arg Asp Ala Leu Leu Ala Lys Cys Ser Asp Tyr Gln Arg 1315 1320 1325 Arg Leu Leu Ser Val Asn Ile Arg Val Arg Val Asp Leu Arg Asp Asn 1330 1335 1340 Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro 1345 1350 1355 1360 Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Asn Cys Gln Phe Val 1365 1370 1375 Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala Glu Asn Glu 1380 1385 1390 Ala Glu Thr Lys Leu His Ala Ile Leu Glu Asp Ile His Ile Asn Leu 1395 1400 1405 Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val Val Ala Asn 1410 1415 1420 Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly Lys Ile Ala Gln 1425 1430 1435 1440 Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr 1445 1450 1455 Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala 1460 1465 1470 Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu Pro Pro Gly 1475 1480 1485 Ala Lys Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe 1490 1495 1500 Gly Arg Ser Tyr 1505 <210> 77 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007129327.1 <400> 77 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu His Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Glu Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ser Phe 145 150 155 160 Gln Ser Val Gly Ala Lys Trp Asn Val Ser Thr Thr Lys Ala Lys Val 165 170 175 Val Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Leu Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Gln 500 505 510 Arg Glu Val Thr Pro Val Asn Ile Pro Glu Ala Arg Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Thr Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Arg Asn Ala Glu Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Glu Arg Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Ile Ser 675 680 685 Pro Tyr Ser Cys Ile Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Ala Lys Asn Glu Ala Ala Ala Pro Leu Lys Thr Arg Ser 725 730 735 Ala Pro Ser Leu Asn Asn Thr Cys Ala Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Leu Gly Asn Pro Pro Ala Thr Ile Val Gln Asn Val Pro Ser Thr Ser 805 810 815 Ser Ser Asp Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Gly Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ser Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Ser Arg Ser Ser Glu Pro Cys Gly Pro 885 890 895 Glu Thr Pro Glu Ala Lys Ser Leu Phe Asp Lys Val Ala Thr Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Val Asp Tyr Lys Pro Val Phe Ala Asn Gly Val Glu Glu 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Thr Gln Lys Gln Asn Asp Gly Gln Lys Lys Asp Phe Ser Lys Asn 980 985 990 Gln Gly Ser Gly Leu Ser Ser Ser Gly Pro Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Leu Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Val Leu Ala Glu Pro Ile Ala Val Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Gly Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu His Ala Leu Leu Glu Asp Ile 1395 1400 1405 His Val Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Leu Gln Lys Glu Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Thr Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Arg Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Gln Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 78 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007172136.1 <400> 78 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Gly Lys Ala Ser Xaa Xaa Xaa 20 25 30 Xaa Xaa Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe Thr 35 40 45 Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr Ala 50 55 60 Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His Trp 65 70 75 80 Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr Ser 85 90 95 Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Ala 100 105 110 Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys 115 120 125 Gly Asn Ala Ala Trp Gln Glu Gln Leu Glu Gln Ser Lys Ala Pro Val 130 135 140 His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe Gln 145 150 155 160 Ser Val Gly Thr Lys Trp Asn Val Ser Thr Thr Lys Ala Lys Val Val 165 170 175 Pro Glu Lys Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly Ala 180 185 190 Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr 195 200 205 Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln 210 215 220 Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro 225 230 235 240 Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala 245 250 255 Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe 260 265 270 Ala Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala 275 280 285 Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln 290 295 300 Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu Gln 305 310 315 320 Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys Cys 325 330 335 Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp 340 345 350 Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn 355 360 365 Lys Tyr Ser Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp 370 375 380 Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp 385 390 395 400 Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys 405 410 415 Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu 420 425 430 Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly 435 440 445 Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly 450 455 460 Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser 465 470 475 480 Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys 485 490 495 Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Glu 500 505 510 Glu Val Val Pro Val Asn Ile Pro Glu Ala Pro Glu Glu Ala Lys Glu 515 520 525 Val Ala Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val Trp 530 535 540 Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu Ser 545 550 555 560 Glu Gly Glu Thr Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile Thr 565 570 575 Lys Ile His Lys Asn Ala Glu Gly Lys Ile Val Ser Leu Asp Ala Lys 580 585 590 Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp 595 600 605 Leu Ala Glu Thr Glu Arg Ala Leu Pro Val Pro Ala Ile Cys Val Thr 610 615 620 Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe 625 630 635 640 Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu Gly 645 650 655 Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln 660 665 670 Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro 675 680 685 Tyr Ser Cys Ile Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp Gly 690 695 700 His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Val 705 710 715 720 Glu Thr Thr Lys Asn Glu Ala Ala Thr Pro Leu Lys Thr Arg Ser Ala 725 730 735 Pro Ser Leu Asn Asn Thr Cys Ala Thr Ser Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Leu 785 790 795 800 Gly Asn Pro Pro Ala Ala Ile Ile Gln Asn Val Ser Ser Lys Ser Ser 805 810 815 Ser Asp Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Thr Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Asn Glu Tyr Ile Pro Gly Gln Pro Thr Ser Ser Gln 865 870 875 880 Ser Ser Asp Ser Ser Pro Ser Arg Ser Ser Glu Pro Arg Gly Pro Glu 885 890 895 Thr Pro Glu Ala Lys Ser Leu Phe Asp Lys Val Ala Thr Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp 915 920 925 Thr Ala Val His Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Thr Gly Val Asp Tyr Lys Pro Val Ser Ala Asn Gly Ala Glu Glu Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Thr Gln Lys Gln Asn Asp Gly Gln Lys Lys Asp Ser Ser Lys Asn Gln 980 985 990 Gly Ser Gly Leu Ser Ser Gly Gly Pro Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr 1025 1030 1035 1040 Asp Ile Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Gly Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1250 1255 1260 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile Ala Lys Cys Asn Asp 1315 1320 1325 Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala 1380 1385 1390 Glu Asn Glu Ala Glu Thr Lys Leu His Ala Ile Leu Glu Asp Ile His 1395 1400 1405 Val Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asn Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Ala Gln Ile Pro Phe Cys Gly Glu Met Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Arg Pro Leu Cys Glu Leu 1475 1480 1485 Gln Pro Gly Ala Gln Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 79 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005335922.1 <400> 79 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Val Val Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Thr Asn Leu Met Glu His Thr Glu Val Asp His 65 70 75 80 Trp Leu Glu Phe Ser Thr Thr Lys Leu Ser Ser Cys Asp Leu Phe Ala 85 90 95 Ser Ala Ile Ser Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Met Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Phe Lys Gln Asn Lys Thr Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Ala Lys Trp Asp Val Ser Thr Asn Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Ile Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Leu Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Val Leu Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Ile Ile 565 570 575 Thr Lys Ile Tyr Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Phe Gly Ser Lys Glu Lys Ala Lys 705 710 715 720 Val Glu Thr Thr Lys Asn Glu Lys Thr Ala Pro Leu Lys Glu Arg Pro 725 730 735 Ala Pro Ser Leu Asn Asn Thr Cys Val Thr Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Ile Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Asp Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Phe Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Thr Thr Val Val Gln Ser Val Ser Ser Thr Ser 805 810 815 Pro Ala Asn Thr Ser Glu Ser Lys Ser Leu Tyr Asp Lys Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Thr 835 840 845 Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Asp Tyr Ile Pro Gly Gln Pro Pro Ser Ser 865 870 875 880 Gln Ser Ser Asp Ser Asn Pro Ile Arg Asn Ser Asp Thr Ala Gly Pro 885 890 895 Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Val Ser Lys Asp Gln Ile 915 920 925 Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Ser Lys Gln Asn Asp Gly Gln Gly Lys Asp Ser Asn Lys Thr 980 985 990 Gln Gly Ser Gly Leu Ser Ser Gly Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ser Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Arg Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Lys Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Cys Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Ile Met Val His Gly Asp Asn Met Gly Leu Ile Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Gln Lys Arg Leu His Ser Val Asn Ile Arg Val Lys Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Ile His Ala Ile Leu Glu Asp Ile 1395 1400 1405 Gln Val Asn Leu Phe Thr Arg Ala Cys Glu Glu Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Leu Glu Asp Leu Gln Lys Val Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Ile Cys Gly Lys Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 80 <211> 1518 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007172135.1 <400> 80 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Gly Lys Ala Ser Xaa Xaa Xaa 20 25 30 Xaa Xaa Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe Thr 35 40 45 Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr Ala 50 55 60 Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His Trp 65 70 75 80 Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr Ser 85 90 95 Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Ala 100 105 110 Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys 115 120 125 Gly Asn Ala Ala Trp Gln Glu Gln Leu Glu Gln Ser Lys Ala Pro Val 130 135 140 His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe Gln 145 150 155 160 Ser Val Gly Thr Lys Trp Asn Val Ser Thr Thr Lys Ala Lys Val Val 165 170 175 Pro Glu Lys Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly Ala 180 185 190 Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr 195 200 205 Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln 210 215 220 Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro 225 230 235 240 Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala 245 250 255 Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe 260 265 270 Ala Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala 275 280 285 Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln 290 295 300 Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu Gln 305 310 315 320 Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys Cys 325 330 335 Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp 340 345 350 Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn 355 360 365 Lys Tyr Ser Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp 370 375 380 Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp 385 390 395 400 Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys 405 410 415 Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu 420 425 430 Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly 435 440 445 Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly 450 455 460 Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser 465 470 475 480 Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys 485 490 495 Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro Arg 500 505 510 Tyr Val Ala Leu Leu Lys Glu Glu Val Val Pro Val Asn Ile Pro Glu 515 520 525 Ala Pro Glu Glu Ala Lys Glu Val Ala Arg His Pro Lys Asn Pro Asp 530 535 540 Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly 545 550 555 560 Ala Asp Ala Glu Thr Leu Ser Glu Gly Glu Thr Val Thr Phe Ile Asn 565 570 575 Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Asn Ala Glu Gly Lys 580 585 590 Ile Val Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys 595 600 605 Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Glu Arg Ala Leu Pro 610 615 620 Val Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro Val 625 630 635 640 Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys 645 650 655 His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys 660 665 670 Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln 675 680 685 Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Ile Glu Ala Pro Cys Ile 690 695 700 Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser Gly 705 710 715 720 Ser Lys Glu Lys Thr Lys Val Glu Thr Thr Lys Asn Glu Ala Ala Thr 725 730 735 Pro Leu Lys Thr Arg Ser Ala Pro Ser Leu Asn Asn Thr Cys Ala Thr 740 745 750 Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Val Gln Gly Asp 755 760 765 Val Val Arg Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp 770 775 780 Ala Ala Val Lys Gln Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys 785 790 795 800 Thr Gly Gln Glu Tyr Lys Leu Gly Asn Pro Pro Ala Ala Ile Ile Gln 805 810 815 Asn Val Ser Ser Lys Ser Ser Ser Asp Ile Leu Glu Ser Lys Ser Leu 820 825 830 Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg Thr Leu Lys Ala 835 840 845 Glu Lys Ala Pro Lys Ala Lys Val Asn Glu Ala Val Glu Cys Leu Leu 850 855 860 Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Asn Glu Tyr Ile Pro 865 870 875 880 Gly Gln Pro Thr Ser Ser Gln Ser Ser Asp Ser Ser Pro Ser Arg Ser 885 890 895 Ser Glu Pro Arg Gly Pro Glu Thr Pro Glu Ala Lys Ser Leu Phe Asp 900 905 910 Lys Val Ala Thr Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys 915 920 925 Ala Ser Lys Asp Gln Val Asp Thr Ala Val His Glu Leu Leu Gln Leu 930 935 940 Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val Asp Tyr Lys Pro Val Ser 945 950 955 960 Ala Asn Gly Ala Glu Glu Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn 965 970 975 Lys Ser Glu Lys Gln Asn Lys Thr Gln Lys Gln Asn Asp Gly Gln Lys 980 985 990 Lys Asp Ser Ser Lys Asn Gln Gly Ser Gly Leu Ser Ser Gly Gly Pro 995 1000 1005 Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala 1010 1015 1020 Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys 1025 1030 1035 1040 Ser Glu Met Ile Glu Tyr Tyr Asp Ile Ser Gly Cys Tyr Ile Leu Arg 1045 1050 1055 Pro Trp Ala Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala 1060 1065 1070 Glu Ile Lys Lys Leu Gly Val Gly Asn Cys Tyr Phe Pro Met Phe Val 1075 1080 1085 Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe Ala 1090 1095 1100 Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu 1105 1110 1115 1120 Pro Ile Ala Val Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr 1125 1130 1135 Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln 1140 1145 1150 Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu 1155 1160 1165 Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr 1170 1175 1180 Phe Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala 1185 1190 1195 1200 Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys 1205 1210 1215 Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu 1220 1225 1230 Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His 1235 1240 1245 His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Ile Phe Glu Asp 1250 1255 1260 Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly 1265 1270 1275 1280 Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp Asn 1285 1290 1295 Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val Val 1300 1305 1310 Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala 1315 1320 1325 Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn 1330 1335 1340 Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys 1345 1350 1355 1360 Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly 1365 1370 1375 Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr 1380 1385 1390 Gly Glu Lys Leu Thr Val Ala Glu Asn Glu Ala Glu Thr Lys Leu His 1395 1400 1405 Ala Ile Leu Glu Asp Ile His Val Asn Leu Phe Thr Arg Ala Ser Glu 1410 1415 1420 Asp Leu Lys Thr His Met Val Val Ala Asn Thr Met Glu Asp Phe Gln 1425 1430 1435 1440 Lys Glu Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu 1445 1450 1455 Met Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp 1460 1465 1470 Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro 1475 1480 1485 Phe Arg Pro Leu Cys Glu Leu Gln Pro Gly Ala Gln Cys Val Cys Gly 1490 1495 1500 Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 81 <211> 1510 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_003419927.1 <400> 81 Met Ala Ala Leu Cys Leu Thr Val Asn Thr Gly Asp Pro Leu Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Ile Lys Asp Asn Val Ser Ile Ser Ile 20 25 30 Glu Glu Gly Arg Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ala Phe 35 40 45 Thr Asp Ser Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Ile Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Phe Lys Gln Ser Lys Ala Pro 130 135 140 Val His Ala Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Ser Lys Trp Asp Ile Ser Thr Thr Lys Pro Lys Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Ile Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Arg Pro Asp Gln Phe Thr Tyr Thr Ser Asn His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Gly Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Thr Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Arg Ile His Lys Asn Thr Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Phe Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Met His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Ala Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys Arg Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Gln Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Arg Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Ala Arg 705 710 715 720 Val Glu Thr Lys Thr Glu Ser Thr Ala Cys Lys Glu Lys Pro Ala Pro 725 730 735 Ser Leu Asn Ser Pro Cys Ala Thr Pro Glu Asp Ser Val Val Leu Tyr 740 745 750 Ser Arg Val Ala Asp Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys 755 760 765 Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu Ala 770 775 780 Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro Gly 785 790 795 800 Asn Pro Pro Ala Ala Ala Ala Glu Asn Val Ser Ser Lys Ser Pro Gly 805 810 815 Ser Val Leu Asp Gly Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly 820 825 830 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Val 835 840 845 Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu 850 855 860 Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Ser Ser Ser Gln Asn 865 870 875 880 Ser Asp Pro Gly Pro Thr Arg Ser Ser Glu Pro Ser Gly Pro Glu Thr 885 890 895 Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly Glu Ala 900 905 910 Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Ala 915 920 925 Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr 930 935 940 Gly Glu Glu Tyr Lys Pro Ile Ser Ala Ile Gly Ala Lys Asp Lys Asp 945 950 955 960 Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Leu 965 970 975 Gln Thr Gln Ser Asp Gly Gln Arg Lys Asp Cys Leu Lys Asn Gln Gly 980 985 990 Ser Glu Gln Ser Ser Gly Gly Pro Gly Glu Gly Gln Gly Leu Lys Lys 995 1000 1005 Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp 1010 1015 1020 Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp 1025 1030 1035 1040 Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ala Ile Trp Glu 1045 1050 1055 Val Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu 1060 1065 1070 Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Gly Ala Leu Glu Lys Glu 1075 1080 1085 Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg 1090 1095 1100 Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr Ser 1105 1110 1115 1120 Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg 1125 1130 1135 Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu 1140 1145 1150 Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln 1155 1160 1165 Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu Val 1170 1175 1180 Leu Gln Ile Leu Asp Leu Tyr Ala Arg Val Tyr Glu Glu Leu Leu Ala 1185 1190 1195 1200 Val Pro Ile Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly 1205 1210 1215 Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Val Ser Ala Thr Gly Arg 1220 1225 1230 Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe Ser Lys 1235 1240 1245 Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln 1250 1255 1260 Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val 1265 1270 1275 1280 Met Thr Leu Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg 1285 1290 1295 Val Ala Cys Ile Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala 1300 1305 1310 Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr 1315 1320 1325 Gln Lys Arg Leu Leu Ser Val Asn Ile Arg Val Lys Val Asp Leu Arg 1330 1335 1340 Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly 1345 1350 1355 1360 Val Pro Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys Gln 1365 1370 1375 Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Thr Glu 1380 1385 1390 Asn Glu Ala Glu Thr Lys Leu Gln Ala Leu Leu Glu Asp Ile His Val 1395 1400 1405 Asn Leu Phe Thr Arg Ala Phe Glu Asp Leu Lys Thr His Met Val Val 1410 1415 1420 Ala Asp Thr Met Glu Asp Phe Gln Lys Val Leu Asp Ser Gly Lys Ile 1425 1430 1435 1440 Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys 1445 1450 1455 Lys Thr Thr Ala Arg Asp Gln Asp Ile Glu Pro Gly Ala Pro Ser Met 1460 1465 1470 Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Arg Glu Leu Gln 1475 1480 1485 Pro Gly Ala Lys Cys Ile Cys Gly Lys Ala Pro Ala Lys Tyr Tyr Thr 1490 1495 1500 Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 82 <211> 1510 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006869760.1 <400> 82 Met Ala Thr Leu Cys Leu Thr Val Asn Thr Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Ile 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Asp Asn Val Leu Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Val Glu Leu Ser Ala Ala Lys Leu Ser Ser Cys Asn Ser Phe Met 85 90 95 Pro Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Thr Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Arg Ala Phe 145 150 155 160 Gln Ser Val Gly Ser Lys Trp Asn Val Ser Thr Thr Arg Pro Lys Val 165 170 175 Ile Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Ile Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Val Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Asn Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Ala Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Ser Lys Tyr Asn Ile Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Arg 500 505 510 Lys Glu Val Ile Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Thr Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Phe Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala His Ala Leu Pro Ile Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Arg Asp Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Ala Lys Asn Glu Thr Thr Pro Asn Lys Glu Lys Ser Ala 725 730 735 Pro Ser Leu Asn Asn Thr Cys Ala Thr Pro Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Ser Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Ala Ala Val Val Gln Asn Ile Ser Ser Lys Ser Ser 805 810 815 Glu Ser Ile Leu Asp Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Asp Tyr Val Pro Gly Gln Pro Pro Leu Ser Gln 865 870 875 880 Asn Ser Gly Pro Ala Thr Arg Asn Ser Glu Pro Thr Gly Pro Gln Thr 885 890 895 Pro Glu Ala Lys Ile Leu Phe Asp Lys Val Ala Phe Gln Gly Asp Val 900 905 910 Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Thr 915 920 925 Ala Val Gln Asp Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr 930 935 940 Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Pro Glu Glu Thr Asp 945 950 955 960 Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro 965 970 975 Gln Lys Gln Ser Asp Gly Gln Arg Lys Asp Ser Ser Lys Ser Gln Gly 980 985 990 Ser Gly Gln Ser Ser Gly Gly Pro Gly Glu Gly Gln Gly Pro Lys Lys 995 1000 1005 Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp 1010 1015 1020 Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp 1025 1030 1035 1040 Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp Glu 1045 1050 1055 Phe Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu 1060 1065 1070 Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys Glu 1075 1080 1085 Lys Ser His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg 1090 1095 1100 Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr Ser 1105 1110 1115 1120 Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg 1125 1130 1135 Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu 1140 1145 1150 Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln 1155 1160 1165 Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Glu Lys Glu Val 1170 1175 1180 Leu Glu Ile Leu Asp Leu Tyr Ala Arg Val Tyr Glu Glu Leu Leu Ala 1185 1190 1195 1200 Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly 1205 1210 1215 Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly Arg 1220 1225 1230 Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe Ser Lys 1235 1240 1245 Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Ala Pro Gly Glu Lys Gln 1250 1255 1260 Phe Ala Phe Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val 1265 1270 1275 1280 Leu Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro His 1285 1290 1295 Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr Asn Ala 1300 1305 1310 Leu Ser Glu Glu Asp Arg Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr 1315 1320 1325 Arg Lys Arg Leu Leu Ser Val Asn Ile Arg Val Arg Val Asp Leu Arg 1330 1335 1340 Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly 1345 1350 1355 1360 Val Pro Ile Arg Leu Glu Leu Gly Pro Arg Asp Met Lys Ser Cys Gln 1365 1370 1375 Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Thr Glu 1380 1385 1390 Asn Glu Ala Glu Thr Thr Leu Gln Thr Ile Leu Glu Asp Ile Gln Ser 1395 1400 1405 Asn Leu Tyr Thr Arg Ala Ser Glu Gly Leu Lys Thr His Met Val Val 1410 1415 1420 Ala Asn Thr Met Glu Asp Phe Gln Lys Ala Leu Asp Ser Gly Lys Ile 1425 1430 1435 1440 Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys 1445 1450 1455 Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met 1460 1465 1470 Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln 1475 1480 1485 Pro Gly Ala Lys Cys Leu Cys Gly Lys Asn Pro Ala Lys Tyr Tyr Thr 1490 1495 1500 Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 83 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007521381.1 <400> 83 Met Ala Thr Leu Ser Leu Thr Val Asn Tyr Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Lys Ile Ala Ile 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Met Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Val Gly Leu Tyr Gly Ser Asn Gln Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser His Ser Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn Leu Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Pro Thr Thr Lys Ala Lys Ala 165 170 175 Ala Ser Glu Lys Lys Gln Asp Val Gly Lys Phe Ile Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Leu Asp Ser Lys His Arg Asn Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Ser Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Leu Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Asp Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Gly Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Asp Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Ser Ile 565 570 575 Thr Lys Ile His Arg Asn Thr Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Val Arg Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Ile Asn Lys Asn Thr Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Phe Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Asn Lys 705 710 715 720 Thr Glu Thr Ile Lys Asn Glu Thr Thr Ala Ser Phe Lys Glu Lys Gln 725 730 735 Thr Pro Pro Leu Asn Asn Ser Cys Ala Val Ser Glu Asp Ser Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp Thr Val Arg Glu Leu Lys 755 760 765 Thr Lys Lys Ala Ala Lys Glu Glu Val Asp Thr Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Ala Ala Thr Gln Asn Ala Ser Leu Gly Ser 805 810 815 Ser Glu Ser Leu Gln Asp Ser Lys Ser Leu Tyr Asn Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ile Ser 865 870 875 880 Gln Ser Ser Asp Ser Lys Pro Asn Lys Asn Ser Glu Pro Ser Gly Pro 885 890 895 Glu Thr Thr Glu Ala Lys Ala Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Val Gly Ala Asp Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Ser 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Gln Lys Lys Asp Leu Ser Lys Asn 980 985 990 Gln Gly Ser Gly Val Ser Pro Ser Gly Ser Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Phe Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Tyr Glu Glu Ala Ala Lys 1170 1175 1180 Glu Val Leu Glu Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Met Phe Glu Asp Pro Lys Thr Ser Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Arg Val Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Ser Val Ala Ile Arg Val Arg Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Leu Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Ser Glu Ala Glu Ala Lys Leu Gln Ala Thr Leu Glu Asp Ile 1395 1400 1405 His Gln Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asp Thr Met Glu Asp Phe Gln Lys Glu Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Lys Pro Gly Ala Lys Cys Ile Cys Ser Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 84 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005335921.1 <400> 84 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Val Val Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Thr Asn Leu Met Glu His Thr Glu Val Asp His 65 70 75 80 Trp Leu Glu Phe Ser Thr Thr Lys Leu Ser Ser Cys Asp Leu Phe Ala 85 90 95 Ser Ala Ile Ser Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Met Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Phe Lys Gln Asn Lys Thr Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Ala Lys Trp Asp Val Ser Thr Asn Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Ile Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Leu Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Val Leu 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Pro 530 535 540 Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Ile Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Ile Ile Ile Thr Lys Ile Tyr Lys Asn Ala Asp Gly 580 585 590 Lys Ile Val Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr His Ala Leu 610 615 620 Pro Val Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys 690 695 700 Ile Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Phe 705 710 715 720 Gly Ser Lys Glu Lys Ala Lys Val Glu Thr Thr Lys Asn Glu Lys Thr 725 730 735 Ala Pro Leu Lys Glu Arg Pro Ala Pro Ser Leu Asn Asn Thr Cys Val 740 745 750 Thr Ser Glu Asp Ser Leu Val Leu Tyr Asn Arg Val Ala Ile Gln Gly 755 760 765 Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Ala Lys Asp Asp Ile 770 775 780 Asp Ala Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Phe Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Thr Thr Val Val 805 810 815 Gln Ser Val Ser Ser Thr Ser Pro Ala Asn Thr Ser Glu Ser Lys Ser 820 825 830 Leu Tyr Asp Lys Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys 835 840 845 Ala Glu Lys Ala Pro Lys Thr Lys Val Asn Glu Ala Val Glu Cys Leu 850 855 860 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Lys Asp Tyr Ile 865 870 875 880 Pro Gly Gln Pro Pro Ser Ser Gln Ser Ser Asp Ser Asn Pro Ile Arg 885 890 895 Asn Ser Asp Thr Ala Gly Pro Glu Thr Pro Glu Ala Lys Val Leu Phe 900 905 910 Asp Lys Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu 915 920 925 Lys Val Ser Lys Asp Gln Ile Asp Thr Ala Val Gln Glu Leu Leu Gln 930 935 940 Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val Glu Tyr Lys Pro Val 945 950 955 960 Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu 965 970 975 Asn Lys Ser Glu Lys Gln Asn Lys Pro Ser Lys Gln Asn Asp Gly Gln 980 985 990 Gly Lys Asp Ser Asn Lys Thr Gln Gly Ser Gly Leu Ser Ser Gly Gly 995 1000 1005 Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Ala Lys Lys Glu Glu Asn Leu Ala Glu Trp Tyr Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Ser Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp 1060 1065 1070 Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Ile Phe 1075 1080 1085 Val Ser Gln Gly Ala Leu Glu Lys Glu Arg Thr His Val Ala Asp Phe 1090 1095 1100 Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala 1105 1110 1115 1120 Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala 1125 1130 1135 Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn 1140 1145 1150 Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe 1155 1160 1165 Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala 1170 1175 1180 Thr Phe Glu Glu Ala Ala Glu Glu Val Leu Lys Ile Leu Glu Leu Tyr 1185 1190 1195 1200 Ala Arg Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg 1205 1210 1215 Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Ile 1220 1225 1230 Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser 1235 1240 1245 His His Leu Gly Gln Asn Phe Ser Lys Met Cys Glu Ile Ile Phe Glu 1250 1255 1260 Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Cys Ser Trp 1265 1270 1275 1280 Gly Leu Thr Thr Arg Thr Ile Gly Val Met Ile Met Val His Gly Asp 1285 1290 1295 Asn Met Gly Leu Ile Leu Pro Pro Arg Val Ala Cys Val Gln Val Val 1300 1305 1310 Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Lys Glu 1315 1320 1325 Ala Leu Ile Ala Lys Cys Asn Asp Tyr Gln Lys Arg Leu His Ser Val 1330 1335 1340 Asn Ile Arg Val Lys Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp 1345 1350 1355 1360 Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val 1365 1370 1375 Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp 1380 1385 1390 Thr Gly Glu Lys Leu Thr Ile Ala Glu Asn Glu Ala Glu Thr Lys Ile 1395 1400 1405 His Ala Ile Leu Glu Asp Ile Gln Val Asn Leu Phe Thr Arg Ala Cys 1410 1415 1420 Glu Glu Leu Lys Thr His Met Val Val Ala Asn Thr Leu Glu Asp Leu 1425 1430 1435 1440 Gln Lys Val Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln 1460 1465 1470 Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Ile Cys 1490 1495 1500 Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 85 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_008148043.1 <400> 85 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Phe Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Met Thr 50 55 60 Ala Arg Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Pro 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Val Ala Trp Gln Glu Gln Leu Gln Gln Asn Ser Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Gly 165 170 175 Ala Pro Gln Lys Lys Pro Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Glu Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Arg Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Thr Glu Ser Lys His Arg Asn Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Pro Phe Gly Gln Thr Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Asn Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Arg Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Val Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Phe Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Gln 500 505 510 Lys Glu Val Ile Pro Val His Val Pro Asp Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Arg Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asn Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala His Ala Leu Pro Ile Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro His Ser Cys Arg Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Val Lys Asn Glu Thr Thr Thr Pro Val Lys Glu Arg Pro 725 730 735 Ala Pro Pro Thr Asn Ala Ser Ala Thr Ser Gly Asp Pro Leu Ala Leu 740 745 750 Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Ala 755 760 765 Lys Lys Ala Ala Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Ser Pro Pro Ala Val Ala Ala Arg Ala Val Ser Ser Pro Ser Pro 805 810 815 Ala Ser Pro Pro Gly Gly Gly Ser Leu Tyr Asp Glu Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys 850 855 860 Glu Lys Thr Gly Gln Glu Tyr Val Pro Gly Gln Pro Pro Leu Pro His 865 870 875 880 Gly Ser Gly Ser Ser Pro Ala Arg Ser Ser Glu Pro Cys Gly Pro Glu 885 890 895 Thr Ala Glu Ala Lys Val Leu Phe Asp Gln Val Ala Ser Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp 915 920 925 Ala Ala Val Gln Glu Leu Leu Gln Arg Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Pro Lys Gln Ser Asp Gly Ser Lys Lys Glu Pro Ala Lys Ser Gln 980 985 990 Gly Gly Gly Gly Leu Pro Pro Ser Gly Ala Gly Glu Gly Pro Gly Pro 995 1000 1005 Arg Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Phe Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Ser Ala Leu Glu 1075 1080 1085 Thr Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Val 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Thr Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile Ala Lys Cys Asn 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Arg Ala Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Xaa Xaa Xaa Xaa Xaa Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Leu Leu Glu Asp Ile 1395 1400 1405 His Ala Asn Leu Phe Arg Lys Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Ser Met Glu Asp Phe Gln Lys Leu Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Arg Cys Val Cys Gly Lys Asn Pro Ala Lys Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 86 <211> 1497 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004439743.1 <400> 86 Met Ala Ala Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Gly Ser Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Phe Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Val Asn Gln Gly Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Glu Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Leu Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Tyr Val Tyr Cys Tyr Leu Tyr Leu Glu Gly Ser Lys Asp Gly 450 455 460 Ser Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe 465 470 475 480 Asn Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu 485 490 495 Lys Glu Glu Val Val Pro Val Ser Ile Pro Glu Ala Gln Glu Glu Met 500 505 510 Lys Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro 515 520 525 Val Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr 530 535 540 Leu Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn 545 550 555 560 Ile Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Leu Ser Leu Asp 565 570 575 Ala Gln Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile 580 585 590 Thr Trp Leu Ala Glu Thr Ala Arg Ala Leu Pro Val Pro Ala Val Cys 595 600 605 Val Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu 610 615 620 Asp Phe Lys Gln Tyr Val Asn Thr Asn Ser Lys His Glu Glu Leu Met 625 630 635 640 Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln 645 650 655 Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val 660 665 670 Ser Pro Tyr Ser Cys Arg Glu Ala Pro Cys Val Leu Ile Phe Ile Pro 675 680 685 Asp Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr 690 695 700 Lys Val Glu Met Thr Lys Ser Glu Thr Ala Thr Pro Phe Lys Glu Lys 705 710 715 720 Pro Ala Pro Ser Leu Asn Asn Thr Cys Ala Thr Ser Glu Glu Ser Leu 725 730 735 Val Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu 740 745 750 Lys Ala Lys Lys Ala Ala Lys Glu Asp Thr Asp Ala Ala Val Lys Gln 755 760 765 Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr 770 775 780 Lys Pro Gly Asn Pro Pro Ala Ala Val Val Gln Asn Val Ser Ser Lys 785 790 795 800 Ser Ser Ala Ser Ile Leu Glu Ile Lys Ser Leu His Asp Ala Val Ala 805 810 815 Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys 820 825 830 Ala Glu Val Asn Glu Ala Val Glu Ser Leu Leu Ser Leu Lys Ala Gln 835 840 845 Tyr Lys Glu Lys Thr Gly Lys Asp Tyr Ile Pro Gly Gln Pro Pro Leu 850 855 860 Ser Gln Ser Ser Asp Ser Ser Pro Ser Arg Ser Ser Glu Pro Ser Gly 865 870 875 880 Pro Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln 885 890 895 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln 900 905 910 Val Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys 915 920 925 Ser Leu Thr Gly Val Glu Tyr Lys Pro Val Ser Ile Thr Gly Ala Glu 930 935 940 Asp Arg Asp Lys Arg Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln 945 950 955 960 Asn Lys Pro Gln Lys Gln Asn Asp Gly Pro Lys Lys Asp Leu Phe Lys 965 970 975 Asn Gln Gly Ser Gly Leu Ser Ser Ser Gly Pro Gly Glu Gly Gln Gly 980 985 990 Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn 995 1000 1005 Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu 1010 1015 1020 Tyr Tyr Asp Val Ser Gly Cys Tyr Val Leu Arg Pro Trp Ala Tyr Ala 1025 1030 1035 1040 Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu 1045 1050 1055 Gly Ile Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu 1060 1065 1070 Glu Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp 1075 1080 1085 Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg 1090 1095 1100 Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln 1105 1110 1115 1120 Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val 1125 1130 1135 Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe 1140 1145 1150 Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala 1155 1160 1165 Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Ile Tyr Glu Glu 1170 1175 1180 Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys 1185 1190 1195 1200 Phe Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala 1205 1210 1215 Ser Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn 1220 1225 1230 Phe Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly 1235 1240 1245 Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr 1250 1255 1260 Ile Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu 1265 1270 1275 1280 Pro Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile 1285 1290 1295 Thr Asn Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile Ala Lys Cys 1300 1305 1310 Asn Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg Val Lys Val 1315 1320 1325 Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu 1330 1335 1340 Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys 1345 1350 1355 1360 Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr 1365 1370 1375 Val Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp 1380 1385 1390 Ile His Val Asn Leu Phe Thr Lys Ala Ser Glu Asp Leu Lys Thr His 1395 1400 1405 Met Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Val Leu Asp Ser 1410 1415 1420 Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp 1425 1430 1435 1440 Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala 1445 1450 1455 Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Arg Pro Leu Cys 1460 1465 1470 Glu Leu Gln Pro Gly Thr Lys Cys Val Cys Gly Lys Thr Pro Ala Lys 1475 1480 1485 Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1490 1495 <210> 87 <211> 1514 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004685497.1 <400> 87 Met Ala Thr Leu Cys Leu Thr Val Asn Thr Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Thr Asn Gln Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Ser Leu Phe Thr 85 90 95 Ser Ala Val Asn Glu Leu Asn Leu Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Phe Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Ser Ala Ala Trp Gln Glu Gln Leu Gln Gln His Gly Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Glu Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Ala Ser Ala Thr Arg Ala Lys Val 165 170 175 Ala Pro Glu Lys Arg Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Arg Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ala Glu Ser Lys His Arg Lys Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Phe Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Ser Ile Pro Glu Ala Arg Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Ile 530 535 540 Trp Tyr Ser Pro Lys Ile Tyr Ile Glu Gly Ala Asp Ala Asp Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Asn Lys Ile His Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr Gln Ala Leu Pro Ile Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys Arg Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Arg Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Lys Asn Glu Val Thr Ser Gly Phe Tyr Arg Asp Glu Arg 725 730 735 Pro Glu Ser Ser Ser Thr Asn Leu Gly Ala Thr Cys Glu Asp Ser Leu 740 745 750 Val Leu Tyr Asn Arg Val Ala Leu Gln Gly Asp Val Val Arg Glu Leu 755 760 765 Lys Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Glu 770 775 780 Leu Leu Thr Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr 785 790 795 800 Lys Pro Gly Ser Pro Pro Ala Ala Ala Ala Gln Asp Ala Pro Cys Lys 805 810 815 Pro Ser Ala Ser Ala Leu Asp Ser Lys Ser Leu Tyr Asp Glu Val Ala 820 825 830 Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys 835 840 845 Ala Lys Val Ser Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln 850 855 860 Tyr Lys Glu Lys Thr Gly Lys Asp Tyr Ile Pro Gly Gln Pro Pro Val 865 870 875 880 Ser Gln Thr Pro Pro Ser Ser Leu Thr Asn Asn Asn Ser Lys Pro Val 885 890 895 Gly Pro Glu Thr Pro Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser 900 905 910 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp 915 920 925 Gln Val Asp Thr Ala Val Gln Glu Leu Leu Gln Leu Lys Ser Gln Tyr 930 935 940 Lys Ser Leu Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Thr 945 950 955 960 Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys 965 970 975 Gln Asn Lys Ser Gln Lys Gln Asn Asp Gly Gln Lys Lys Glu Pro Ser 980 985 990 Lys Asn Gln Gly Ser Gly Pro Ser Ser Gly Gly Ser Gly Glu Gly Gln 995 1000 1005 Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu 1010 1015 1020 Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile 1025 1030 1035 1040 Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Phe 1045 1050 1055 Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys 1060 1065 1070 Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala 1075 1080 1085 Leu Glu Thr Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala 1090 1095 1100 Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile 1105 1110 1115 1120 Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ser Tyr Ala Lys Trp Val 1125 1130 1135 Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val 1140 1145 1150 Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu 1155 1160 1165 Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr Tyr Glu Glu Ala 1170 1175 1180 Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu 1185 1190 1195 1200 Asp Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu 1205 1210 1215 Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Leu Glu Ala Phe Ile Ser 1220 1225 1230 Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln 1235 1240 1245 Asn Phe Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Ala Pro 1250 1255 1260 Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg 1265 1270 1275 1280 Thr Ile Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val 1285 1290 1295 Leu Pro Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly 1300 1305 1310 Ile Thr Asn Ala Leu Ala Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys 1315 1320 1325 Cys Asn Glu Tyr Arg Arg Arg Leu Leu Ser Val Asn Val Arg Val Arg 1330 1335 1340 Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp 1345 1350 1355 1360 Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met 1365 1370 1375 Lys Thr Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu 1380 1385 1390 Thr Val Ala Glu His Glu Ala Glu Thr Lys Leu Gln Ala Leu Leu Glu 1395 1400 1405 Asp Ile His Ile Ser Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Ala 1410 1415 1420 His Met Val Val Ala Asn Thr Met Glu Glu Phe Gln Lys Ala Leu Asp 1425 1430 1435 1440 Ser Gly Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu 1445 1450 1455 Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly 1460 1465 1470 Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu 1475 1480 1485 His Glu Leu Gln Pro Gly Ala Arg Cys Val Cys Gly Lys Asn Thr Ala 1490 1495 1500 Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 88 <211> 1510 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004578688.1 <400> 88 Met Ala Ala Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Leu Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Met Ala Arg Val Ala Asn Arg 50 55 60 Val Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Met Phe Pro 85 90 95 Ser Ala Val Ser Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Ser Thr Ala Trp Gln Glu Gln Leu Lys Glu Asn Lys Thr Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Asp Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Ser Lys Trp Gly Val Ser Val Asn Lys Ala Asn Lys 165 170 175 Ala Pro Glu Lys Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Leu Glu Ser Lys His Arg Arg Asn Ser Ile Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Thr Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Asn Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Ser Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Ile Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Leu Ala Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Leu Thr 595 600 605 Trp Leu Ala Glu Thr Ala His Ala Leu Pro Ile Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asp Ser Lys Arg Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Arg Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Phe Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Gly Glu Ala Ile Lys Thr Glu Lys Thr Pro Val Lys Glu Lys Pro Ala 725 730 735 Pro Ala Leu Thr Ser Ala Cys Ala Ala Ser Glu Asp Pro Val Leu Leu 740 745 750 Tyr Ser Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Thr 755 760 765 Lys Lys Ala Ala Lys Glu Asp Ile Asp Thr Ala Val Lys Lys Leu Leu 770 775 780 Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Ala Ala Val Gly His Ser Ser Ser Thr Ser Ser Ala 805 810 815 Ser Val Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly 820 825 830 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Val 835 840 845 Asn Glu Ala Val Glu Arg Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu 850 855 860 Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Leu Ser Gln Ser 865 870 875 880 Thr Asp Ser Lys Pro Ala Gly Asn Ser Glu Pro Ala Gly Pro Glu Thr 885 890 895 Ser Asp Ala Lys Ala Leu Phe Asp Arg Val Ala Cys Gln Gly Glu Val 900 905 910 Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Ile Asp Ala 915 920 925 Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr 930 935 940 Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp 945 950 955 960 Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro 965 970 975 Gln Lys Gln Asn Asp Gly Gln Lys Lys Asp Ala Ser Lys Ser Gln Gly 980 985 990 Ser Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys 995 1000 1005 Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp 1010 1015 1020 Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp 1025 1030 1035 1040 Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp Glu 1045 1050 1055 Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu 1060 1065 1070 Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ser Ala Leu Glu Lys Glu 1075 1080 1085 Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg 1090 1095 1100 Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser 1105 1110 1115 1120 Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg 1125 1130 1135 Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu 1140 1145 1150 Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln 1155 1160 1165 Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu Val 1170 1175 1180 Leu Gln Ile Leu Asp Leu Tyr Val Arg Val Tyr Glu Glu Leu Leu Ala 1185 1190 1195 1200 Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly 1205 1210 1215 Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ser Ser Gly Arg 1220 1225 1230 Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser Lys 1235 1240 1245 Met Cys Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln 1250 1255 1260 Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val 1265 1270 1275 1280 Met Val Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg 1285 1290 1295 Val Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala 1300 1305 1310 Leu Ser Glu Glu Asp Arg Glu Ala Leu Val Ala Lys Cys Asn Asp Tyr 1315 1320 1325 Arg Arg Arg Leu Leu Ser Val Ser Ile Arg Val Arg Ala Asp Leu Arg 1330 1335 1340 Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly 1345 1350 1355 1360 Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Asn Cys Gln 1365 1370 1375 Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala Glu 1380 1385 1390 Asn Glu Ala Glu Thr Lys Leu Gln Thr Ile Leu Glu Asp Ile Gln Ala 1395 1400 1405 Asn Leu Phe Ala Arg Ala Ser Glu Asp Leu Arg Thr His Met Val Val 1410 1415 1420 Ala Asp Thr Met Glu Glu Phe Gln Lys Ile Leu Asp Ser Gly Lys Ile 1425 1430 1435 1440 Ala Gln Ile Pro Phe Cys Gly Glu Ile Glu Cys Glu Asp Trp Ile Lys 1445 1450 1455 Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser Met 1460 1465 1470 Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Arg Glu Leu Gln 1475 1480 1485 Pro Gly Ala Lys Cys Ile Cys Gly Lys Asn Pro Ala Lys Phe Tyr Thr 1490 1495 1500 Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 89 <211> 1452 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005879946.1 <400> 89 Met Thr Ala Lys Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile 1 5 10 15 Asp His Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu 20 25 30 Phe Pro Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr 35 40 45 Tyr Leu Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala 50 55 60 Thr Leu Lys Gly Asn Val Ala Trp Gln Glu Gln Leu Gln Gln Asn Arg 65 70 75 80 Ala Pro Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln 85 90 95 Ala Phe Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala 100 105 110 Lys Gly Ala Pro Gln Lys Lys Pro Asp Val Gly Lys Phe Val Glu Leu 115 120 125 Pro Gly Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala 130 135 140 Ser Gly Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln 145 150 155 160 His Tyr Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp 165 170 175 Thr Asn Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu 180 185 190 Asp Val Ala Met Leu His Ile Glu Pro Asp Gln Phe Thr Tyr Thr Ser 195 200 205 Asp His Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu 210 215 220 Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala Glu Arg Met Lys Ala Glu 225 230 235 240 Arg Glu Gln Arg Thr Glu Ser Lys His Arg Asn Asn Ser Val Glu Lys 245 250 255 Asn Leu Gln Met Trp Glu Glu Met Lys Lys Gly Ser Pro Phe Gly Gln 260 265 270 Ser Cys Cys Leu Arg Ala Lys Ile Asp Met Asn Ser Asn Asn Gly Cys 275 280 285 Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg 290 295 300 Thr Gly Asn Arg Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro 305 310 315 320 Ile Val Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu 325 330 335 Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly 340 345 350 Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn 355 360 365 Thr Val Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asp Glu Gly Leu 370 375 380 Val Asp Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu 385 390 395 400 Arg Arg Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln 405 410 415 Gly Ser Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala 420 425 430 Phe Asn Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu 435 440 445 Leu Lys Lys Glu Val Ile Pro Val His Val Pro Gly Ala Gln Glu Glu 450 455 460 Met Lys Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys 465 470 475 480 Pro Val Trp Tyr Ser Pro Arg Val Phe Ile Glu Gly Ala Asp Ala Glu 485 490 495 Thr Leu Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile 500 505 510 Asn Ile Thr Lys Ile His Lys Asn Ala Asn Gly Lys Ile Val Ser Leu 515 520 525 Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys 530 535 540 Ile Thr Trp Leu Ala Glu Thr Ala His Ala Leu Pro Ile Pro Ala Ile 545 550 555 560 Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp 565 570 575 Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu 580 585 590 Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile 595 600 605 Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro 610 615 620 Val Ser Pro His Ser Cys Arg Glu Ala Pro Cys Val Leu Ile Phe Ile 625 630 635 640 Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys 645 650 655 Thr Lys Val Glu Thr Val Lys Asn Glu Thr Thr Thr Pro Val Lys Glu 660 665 670 Arg Pro Val Ala Pro Leu Thr Asn Thr Ser Ala Ala Ser Glu Gly Pro 675 680 685 Met Val Leu Tyr Asn Lys Val Ala Ala Gln Gly Asp Val Val Arg Glu 690 695 700 Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp Val Asp Ala Ala Val Lys 705 710 715 720 His Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu 725 730 735 Tyr Lys Pro Gly Ser Pro Pro Ala Ala Ala Ala Ala Gln Ala Val Ser 740 745 750 Ser Pro Ser Ser Ala Ser Pro Pro Gly Gly Arg Ser Leu Tyr Asp Glu 755 760 765 Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala 770 775 780 Pro Lys Ala Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys 785 790 795 800 Ala Gln Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Val Pro Gly Gln Pro 805 810 815 Pro Leu Pro Leu Gly Ser Gly Ser Ser Pro Ala Arg Ser Ser Glu Pro 820 825 830 Cys Gly Pro Glu Thr Ala Glu Ala Lys Val Leu Phe Asp Gln Val Ala 835 840 845 Ser Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys 850 855 860 Asp Gln Val Asp Ala Ala Val Gln Glu Leu Leu Gln Arg Lys Ala Gln 865 870 875 880 Tyr Lys Ser Leu Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly 885 890 895 Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu 900 905 910 Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Ser Lys Lys Glu Pro 915 920 925 Ser Arg Gly Gln Gly Gly Gly Gly Leu Pro Pro Ser Gly Ala Gly Glu 930 935 940 Gly Gln Gly Pro Arg Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys 945 950 955 960 Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu 965 970 975 Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp 980 985 990 Ala Phe Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile 995 1000 1005 Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln 1010 1015 1020 Ser Ala Leu Glu Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu 1025 1030 1035 1040 Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile 1045 1050 1055 Ala Val Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys 1060 1065 1070 Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys 1075 1080 1085 Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr 1090 1095 1100 Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu 1105 1110 1115 1120 Glu Ala Ala Ala Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val 1125 1130 1135 Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu 1140 1145 1150 Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe 1155 1160 1165 Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu 1170 1175 1180 Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys 1185 1190 1195 1200 Met Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr 1205 1210 1215 Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly Asp Asn Met Gly 1220 1225 1230 Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro 1235 1240 1245 Cys Gly Ile Thr Thr Ala Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile 1250 1255 1260 Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser Val Asn Ile Arg 1265 1270 1275 1280 Ala Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn 1285 1290 1295 His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg 1300 1305 1310 Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu 1315 1320 1325 Lys Leu Thr Val Thr Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Leu 1330 1335 1340 Leu Glu Asp Ile His Ala Asn Leu Phe Arg Lys Ala Ser Glu Asp Leu 1345 1350 1355 1360 Lys Thr His Met Val Val Ala Asn Ser Met Glu Asp Phe Gln Lys Leu 1365 1370 1375 Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp 1380 1385 1390 Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu 1395 1400 1405 Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys 1410 1415 1420 Pro Leu Cys Glu Leu Gln Pro Gly Ala Arg Cys Val Cys Gly Lys Asn 1425 1430 1435 1440 Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1445 1450 <210> 90 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_008825021.1 <400> 90 Met Ala Ala Leu Ser Leu Thr Val Asp Ala Gly Ala Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Ala Ala Glu His Val Lys Gly Asp Val Ser Val Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Leu Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Thr Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Val Asp Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Val Trp Gln Glu Gln Leu Lys Gln Asn Lys Thr Pro 130 135 140 Ile His Val Lys Arg Trp Phe Ser Phe Leu Glu Ala His Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Ala Lys Trp Asp Val Ser Ala Asn Lys Ala Arg Val 165 170 175 Val Pro Asp Lys Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Val Glu Arg Glu 290 295 300 Gln Arg Thr Glu Ser Arg His Arg Glu Asn Ser Val Glu Arg Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Tyr Val Pro Glu Ala Arg Glu Glu Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Leu Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ser His Thr Pro Pro Val Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Arg Asp Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Lys Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Arg Lys Glu Thr Leu Ser Pro Leu Lys Glu Lys Leu 725 730 735 Ala Ala Ser Leu Asn Asn Ser Cys Ala Thr Thr Glu Asp Ser Ser Val 740 745 750 Leu Tyr Lys Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Ala Val Leu Gln Asn Val Ser Thr Lys Ser 805 810 815 Ser Ser Ala Val Glu Asn Thr Ser Leu Tyr Asp Lys Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Asp Tyr Val Pro Gly Gln Pro Pro Ser Ser Gln 865 870 875 880 Ser Ser Asn Pro Ser Leu Gly Ser Asn Ala Asp Pro Ala Gly Pro Glu 885 890 895 Thr Pro Glu Ala Lys Val Leu Phe Asp Gln Val Ala Cys Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp 915 920 925 Ala Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Thr Gly Val Glu Tyr Lys Pro Val Thr Ala Thr Gly Ala Glu Asp Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Gln Asn Asp Gly Gln Gly Lys Asp Ser Ser Arg Asn Gln 980 985 990 Gly Ser Gly Gly Leu Ser Pro Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ser Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Glu Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Cys Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Ile Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Asp Asp Arg Glu Ala Leu Met Ala Lys Cys Ser 1315 1320 1325 Glu Tyr Gln Arg Arg Leu Leu Ser Ala Asn Ile Arg Val Arg Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Thr 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile 1380 1385 1390 Ala Glu Lys Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 Gln Leu Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Arg Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Leu Glu Asp Phe Gln Lys Ala Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Val Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Lys Cys Ile Cys Gly Lys Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 91 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_008825020.1 <400> 91 Met Ala Ala Leu Ser Leu Thr Val Asp Ala Gly Ala Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Ala Ala Glu His Val Lys Gly Asp Val Ser Val Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Leu Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Thr Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Val Asp Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Val Trp Gln Glu Gln Leu Lys Gln Asn Lys Thr Pro 130 135 140 Ile His Val Lys Arg Trp Phe Ser Phe Leu Glu Ala His Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Ala Lys Trp Asp Val Ser Ala Asn Lys Ala Arg Val 165 170 175 Val Pro Asp Lys Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Val Glu Arg Glu 290 295 300 Gln Arg Thr Glu Ser Arg His Arg Glu Asn Ser Val Glu Arg Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Tyr Val Pro 515 520 525 Glu Ala Arg Glu Glu Met Lys Glu Val Ala Arg His Pro Lys Asn Pro 530 535 540 Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Ile Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Asn Ala Asp Gly 580 585 590 Lys Ile Leu Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Ser His Thr Pro 610 615 620 Pro Val Pro Ala Val Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Arg Asp Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys 690 695 700 Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Lys Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Thr Thr Arg Lys Glu Thr Leu 725 730 735 Ser Pro Leu Lys Glu Lys Leu Ala Ala Ser Leu Asn Asn Ser Cys Ala 740 745 750 Thr Thr Glu Asp Ser Ser Val Leu Tyr Lys Arg Val Ala Val Gln Gly 755 760 765 Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp Val 770 775 780 Asp Ala Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ala Ala Val Leu 805 810 815 Gln Asn Val Ser Thr Lys Ser Ser Ser Ala Val Glu Asn Thr Ser Leu 820 825 830 Tyr Asp Lys Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala 835 840 845 Glu Lys Ala Pro Lys Ala Lys Val Asn Glu Ala Val Glu Cys Leu Leu 850 855 860 Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Lys Asp Tyr Val Pro 865 870 875 880 Gly Gln Pro Pro Ser Ser Gln Ser Ser Asn Pro Ser Leu Gly Ser Asn 885 890 895 Ala Asp Pro Ala Gly Pro Glu Thr Pro Glu Ala Lys Val Leu Phe Asp 900 905 910 Gln Val Ala Cys Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys 915 920 925 Ala Ser Lys Asp Gln Val Asp Ala Ala Val Gln Glu Leu Leu Gln Leu 930 935 940 Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val Glu Tyr Lys Pro Val Thr 945 950 955 960 Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn 965 970 975 Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Gln Gly 980 985 990 Lys Asp Ser Ser Arg Asn Gln Gly Ser Gly Gly Leu Ser Pro Ser Gly 995 1000 1005 Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Ala Lys Lys Glu Glu Asn Leu Ala Glu Trp Tyr Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Ser Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp 1060 1065 1070 Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Ile Phe 1075 1080 1085 Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe 1090 1095 1100 Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala 1105 1110 1115 1120 Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala 1125 1130 1135 Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn 1140 1145 1150 Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe 1155 1160 1165 Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala 1170 1175 1180 Thr Phe Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Glu Leu Tyr 1185 1190 1195 1200 Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly Arg 1205 1210 1215 Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Ile 1220 1225 1230 Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser 1235 1240 1245 His His Leu Gly Gln Asn Phe Ser Lys Met Cys Glu Ile Ile Phe Glu 1250 1255 1260 Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Cys Ser Trp 1265 1270 1275 1280 Gly Leu Thr Thr Arg Thr Ile Gly Val Met Ile Met Val His Gly Asp 1285 1290 1295 Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val 1300 1305 1310 Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Asp Asp Arg Glu 1315 1320 1325 Ala Leu Met Ala Lys Cys Ser Glu Tyr Gln Arg Arg Leu Leu Ser Ala 1330 1335 1340 Asn Ile Arg Val Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp 1345 1350 1355 1360 Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val 1365 1370 1375 Gly Pro Arg Asp Met Lys Thr Cys Gln Phe Val Ala Val Arg Arg Asp 1380 1385 1390 Thr Gly Glu Lys Leu Thr Ile Ala Glu Lys Glu Ala Glu Thr Lys Leu 1395 1400 1405 Gln Ala Ile Leu Glu Asp Ile Gln Leu Asn Leu Phe Thr Arg Ala Ser 1410 1415 1420 Glu Asp Leu Arg Thr His Met Val Val Ala Asn Thr Leu Glu Asp Phe 1425 1430 1435 1440 Gln Lys Ala Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln 1460 1465 1470 Asp Val Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Ile Cys 1490 1495 1500 Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 92 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004699962.1 <400> 92 Met Ala Thr Leu Cys Leu Thr Val Asn Pro Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Ile Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Met Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Ala 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Met Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn Leu Phe Thr 85 90 95 Ser Ala Val Asp Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Val Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Lys Ala Thr Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ser Gln Lys Ala Phe 145 150 155 160 Gln Ser Val Gly Ser Lys Trp Asp Val Ser Pro Thr Arg Pro Lys Val 165 170 175 Val Pro Glu Gln Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro 180 185 190 Gly Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser 195 200 205 Gly Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His 210 215 220 Tyr Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr 225 230 235 240 Asn Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp 245 250 255 Val Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp 260 265 270 His Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly 275 280 285 Lys Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Val Glu Arg 290 295 300 Glu Gln Arg Ile Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn 305 310 315 320 Leu Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser 325 330 335 Cys Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met 340 345 350 Arg Asp Pro Ala Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr 355 360 365 Gly Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile 370 375 380 Val Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr 385 390 395 400 His Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile 405 410 415 Arg Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr 420 425 430 Val Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val 435 440 445 Asp Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg 450 455 460 Arg Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly 465 470 475 480 Ser Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe 485 490 495 Asn Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu 500 505 510 Lys Lys Glu Val Ile Pro Val Asn Ile Pro Glu Ala Gln Glu Asp Met 515 520 525 Lys Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro 530 535 540 Val Trp Tyr Ser Pro Lys Val Leu Ile Glu Gly Ala Asp Ala Glu Thr 545 550 555 560 Leu Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn 565 570 575 Ile Thr Lys Ile His Lys Thr Lys Asp Gly Lys Ile Val Ser Leu Asp 580 585 590 Ala Lys Leu Asn Leu Glu Asn Lys Asp Phe Lys Lys Thr Thr Lys Ile 595 600 605 Thr Trp Leu Ala Glu Thr Gly Arg Ala Leu Pro Val Pro Ala Val Cys 610 615 620 Val Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu 625 630 635 640 Asp Phe Lys Gln Tyr Val Asn Lys Ser Ser Lys Arg Glu Glu Leu Met 645 650 655 Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln 660 665 670 Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val 675 680 685 Ser Pro Tyr Ser Cys Arg Glu Ala Pro Cys Ile Leu Val Tyr Ile Pro 690 695 700 Asp Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Asp Lys Asn 705 710 715 720 Lys Val Glu Thr Thr Lys Ser Glu Thr Thr Pro Cys Lys Glu Lys Gln 725 730 735 Ser Leu Ser Leu Asp Asn Asn Tyr Ala Ala Pro Val Asp Ser Met Ala 740 745 750 Leu Tyr Asn Gln Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Val Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Lys Pro Pro Ala Ala Val Ala Ser Ser Ser Thr Ser Ser Asn 805 810 815 Ser Pro His Ser Val Arg Asp Gly Lys Ser Leu Tyr Asp Glu Val Ala 820 825 830 Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys 835 840 845 Ala Lys Val Thr Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln 850 855 860 Tyr Lys Glu Thr Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Ala Leu 865 870 875 880 Ser Gln Asn Ser Ala Ser Glu Ser Ser Ser Glu Pro Ser Gly Pro Glu 885 890 895 Thr Pro Glu Ala Lys Gly Leu Phe Asp Gln Val Ala Ser Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Gly Glu Lys Ala Pro Lys Asp Gln Val Asp 915 920 925 Arg Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ser Glu Asp Lys 945 950 955 960 Asp Lys Arg Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Gln Asn Asp Gly Gln Lys Lys Asp Ser Ser Lys Asn Gln 980 985 990 Gly Ser Gly Gln Ser Ser Gly Gly Pro Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Asn Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys 1075 1080 1085 Glu Lys Asn His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Lys Glu 1170 1175 1180 Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Arg Thr Pro Gly Glu Lys 1250 1255 1260 Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Leu Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Arg Glu Ala Leu Ile Ala Lys Cys Asn Asp 1315 1320 1325 Tyr Gln Lys Arg Leu Leu Ser Val Asn Ile Arg Val Arg Thr Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Ile Lys Ser Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Thr 1380 1385 1390 Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile Gln 1395 1400 1405 Val Asn Leu Tyr Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Ala 1410 1415 1420 Val Ala Asn Thr Val Glu Asp Phe Gln Lys Ala Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1475 1480 1485 Gln Leu Gly Ala Lys Cys Ile Cys Gly Lys Asn Leu Ala Lys Tyr Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 93 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006140298.1 <400> 93 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asn Asp Ile Arg Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Val Leu Arg Val Ser Glu Asn Val Met Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Thr Gly Leu Tyr Gly Ser Asn Met Met Glu His Ala Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Cys Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asp Leu Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Lys Gln Asn Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Val Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Thr Thr Lys Thr Thr Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Met Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Ser Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Ser Arg Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Thr Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr His Ala Leu Pro Ile Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Thr Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Lys Asn Glu Thr Thr Pro Leu Phe Lys Gly Arg Pro Ala 725 730 735 Pro Ser Leu Asn Asn Thr Cys Ala Thr Ser Glu Asp Ser Leu Val Leu 740 745 750 Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Thr 755 760 765 Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ala Leu Lys Ala Glu Phe Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Ala Ala Val Val Glu Asn Ile Ser Ser Lys Ser Ser 805 810 815 Ser Ile Leu Glu Ser Lys Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly 820 825 830 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Val 835 840 845 Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu 850 855 860 Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Cys Ser Gln Ser 865 870 875 880 Ser Asp Ser Ser Pro Ala Arg Asn Ser Glu Pro Ala Gly Pro Glu Thr 885 890 895 Pro Glu Ala Lys Val Leu Phe Glu Lys Val Ala Ser Gln Gly Glu Ala 900 905 910 Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Ile Asp Pro 915 920 925 Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr 930 935 940 Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp 945 950 955 960 Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro 965 970 975 Gln Lys Gln Asn Asp Gly Gln Arg Lys Glu Ser Ser Lys Asn Gln Gly 980 985 990 Ser Gly Leu Ser Ser Gly Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys 995 1000 1005 Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp 1010 1015 1020 Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp 1025 1030 1035 1040 Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp Glu 1045 1050 1055 Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu 1060 1065 1070 Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu Lys Glu 1075 1080 1085 Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg 1090 1095 1100 Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser 1105 1110 1115 1120 Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg 1125 1130 1135 Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu 1140 1145 1150 Phe Lys His Pro Gln Pro Leu Leu Arg Thr Arg Glu Phe Leu Trp Gln 1155 1160 1165 Glu Gly His Ser Ala Phe Ala Ser Phe Glu Glu Ala Ala Glu Glu Val 1170 1175 1180 Leu Gln Ile Leu Asp Leu Tyr Ala Arg Val Tyr Glu Glu Leu Leu Ala 1185 1190 1195 1200 Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly 1205 1210 1215 Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly Arg 1220 1225 1230 Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser Lys 1235 1240 1245 Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln 1250 1255 1260 Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val 1265 1270 1275 1280 Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg 1285 1290 1295 Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala 1300 1305 1310 Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys Asn Asp Tyr 1315 1320 1325 Arg Arg Arg Leu Leu Ser Ala Asp Ile Arg Val Arg Val Asp Leu Arg 1330 1335 1340 Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Lys 1345 1350 1355 1360 Gly Lys Leu Lys Thr Arg Glu Val Val Ser Gly Ile Gly Arg Ser Glu 1365 1370 1375 Met Phe Ser Arg Asp Leu Thr Ala Met Thr Glu Val Asn Val Ile Tyr 1380 1385 1390 Leu Cys Ala Arg Arg Pro Ser Met Cys Leu Lys Leu Thr Ile Ala Gln 1395 1400 1405 His Cys Phe Val Phe Ile Leu Phe Ile Leu Phe Leu Ala Arg Ala Ser 1410 1415 1420 Glu Asp Leu Lys Thr His Met Ile Val Ala Asn Ser Met Glu Asp Phe 1425 1430 1435 1440 Gln Lys Ala Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln 1460 1465 1470 Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Lys Cys Ile Cys 1490 1495 1500 Asp Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 94 <211> 1556 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007642504.1 <400> 94 Met Leu Ala Arg Gly Ser Leu Leu Thr Pro Arg Ala Pro Val Val Glu 1 5 10 15 Ala Arg Ser Ile Pro Asn Arg Ser Phe Gln Gly Ala Ser Ala Ile Ala 20 25 30 Leu Asp Phe Arg Lys Ser Ala Phe Ser Leu Leu Trp Glu Ile Ala Arg 35 40 45 Asn His Gly Asn Lys Ser Leu Val Leu Ser Phe Leu Asp Ala Leu Leu 50 55 60 Ala Val Glu His Val Lys Gly Asp Val Ser Val Ser Val Glu Glu Gly 65 70 75 80 Lys Glu Asn Leu Leu Arg Val Ser Glu Asp Val Val Phe Thr Asp Ile 85 90 95 Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr Ser Gly Leu 100 105 110 Tyr Gly Thr Asn Leu Met Glu His Thr Glu Ile Asp His Trp Leu Glu 115 120 125 Phe Ser Ala Thr Lys Leu Ser Ser Cys Ala Ala Leu Thr Ser Ala Leu 130 135 140 Thr Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn 145 150 155 160 Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn 165 170 175 Ala Ala Trp Gln Glu Gln Leu Glu Gln Asn Lys Thr Leu Val His Val 180 185 190 Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe Arg Ser Val 195 200 205 Gly Thr Lys Trp Asp Val Ser Glu Asn Lys Ala Arg Val Val Pro Asp 210 215 220 Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met 225 230 235 240 Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His 245 250 255 Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn 260 265 270 Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys 275 280 285 Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu 290 295 300 His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr 305 310 315 320 Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val 325 330 335 Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Ala 340 345 350 Glu Ser Lys His Arg Gln Asn Ser Val Glu Lys Asn Leu Gln Met Trp 355 360 365 Glu Glu Met Lys Lys Gly Ser Pro Phe Gly Gln Ser Cys Cys Leu Arg 370 375 380 Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr 385 390 395 400 Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr 405 410 415 Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile 420 425 430 Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp 435 440 445 Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr 450 455 460 Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys 465 470 475 480 Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp 485 490 495 Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr 500 505 510 Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser 515 520 525 Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Val 530 535 540 Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val 545 550 555 560 Val Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys Glu Val Ala 565 570 575 Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val Trp Tyr Ser 580 585 590 Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly 595 600 605 Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile 610 615 620 His Lys Asn Ala Asp Gly Lys Ile Thr Ser Leu Asp Ala Lys Leu Asn 625 630 635 640 Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala 645 650 655 Glu Thr Thr His Ala Leu Pro Ile Pro Ala Ile Cys Val Thr Tyr Glu 660 665 670 His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys Gln 675 680 685 Tyr Val Asn Lys Asp Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro 690 695 700 Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg 705 710 715 720 Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser 725 730 735 Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp Gly His Thr 740 745 750 Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Ala Glu Pro 755 760 765 Leu Lys Lys Glu Thr Ser Ser Ala Pro Lys Glu Gly Pro Val Pro Ala 770 775 780 Val Ser Pro Cys Ala Ala Ser Glu Glu Ser Ser Val Leu Tyr Asn Arg 785 790 795 800 Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala 805 810 815 Ala Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu Leu Ala Leu Lys 820 825 830 Ala Glu Tyr Lys Gln Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro 835 840 845 Pro Ser Ala Ala Ala Gln Ser Ala Ser Thr Lys Ser Leu Pro Ser Ala 850 855 860 Gly Glu Asp Arg Ser Leu Tyr Asp Lys Ile Ala Ala Gln Gly Glu Val 865 870 875 880 Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Val Thr Glu 885 890 895 Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr 900 905 910 Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ala Ser Gln Lys Ser Gln 915 920 925 Pro Ser Pro Ala Ser Lys Ala Glu Pro Ala Gly Pro Glu Thr Thr Glu 930 935 940 Ala Lys Ala Leu Phe Asp Arg Val Ala Cys Gln Gly Glu Val Val Arg 945 950 955 960 Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Pro Ala Val 965 970 975 Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly Ile 980 985 990 Glu Tyr Lys Pro Val Ser Ala Thr Gly Ser Glu Asp Lys Asp Lys Lys 995 1000 1005 Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys 1010 1015 1020 Gln Asn Asp Gly Pro Gly Lys Asp Ser Ser Lys Ser Gln Gly Gly Gly 1025 1030 1035 1040 Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr 1045 1050 1055 Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Glu Trp Tyr 1060 1065 1070 Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser 1075 1080 1085 Gly Cys Tyr Ile Leu Arg Pro Trp Ser Tyr Ser Ile Trp Glu Ser Ile 1090 1095 1100 Lys Asp Phe Phe Asp Thr Glu Ile Lys Lys Leu Gly Val Glu Asn Cys 1105 1110 1115 1120 Tyr Phe Pro Ile Phe Val Ser Gln Ala Ala Leu Glu Lys Glu Lys Ser 1125 1130 1135 His Ile Glu Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly 1140 1145 1150 Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr 1155 1160 1165 Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu 1170 1175 1180 Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys 1185 1190 1195 1200 His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly 1205 1210 1215 His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Asp Glu Val Met Gln 1220 1225 1230 Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu Leu Leu Ala Ile Pro 1235 1240 1245 Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp 1250 1255 1260 Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile 1265 1270 1275 1280 Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser Lys Met Cys 1285 1290 1295 Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala 1300 1305 1310 Phe Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Ile 1315 1320 1325 Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala 1330 1335 1340 Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser 1345 1350 1355 1360 Glu Glu Asp Arg Glu Ala Leu Met Ala Lys Cys Asn Glu Tyr Arg Lys 1365 1370 1375 Arg Leu Leu Gly Val Asn Ile Arg Val Arg Val Asp Leu Arg Asp Asn 1380 1385 1390 Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro 1395 1400 1405 Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val 1410 1415 1420 Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala Glu Lys Glu 1425 1430 1435 1440 Ala Glu Ser Lys Leu Gln Glu Ile Leu Glu Asp Ile Gln Leu Asn Leu 1445 1450 1455 Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val Val Ser Asn 1460 1465 1470 Thr Leu Glu Asp Phe Gln Lys Val Leu Asp Ser Gly Lys Ile Ala Gln 1475 1480 1485 Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr 1490 1495 1500 Thr Ala Arg Asp Gln Asp Val Glu Pro Gly Ala Pro Ser Met Gly Ala 1505 1510 1515 1520 Lys Ser Leu Cys Ile Pro Phe Thr Pro Leu Cys Glu Leu Gln Pro Gly 1525 1530 1535 Ala Met Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe 1540 1545 1550 Gly Arg Ser Tyr 1555 <210> 95 <211> 1505 <212> PRT <213> Artificial Sequence <220> <223> EPRS ERE73005.1 <400> 95 Met Ala Leu Thr Arg Ser Leu Phe Leu Tyr Ala Leu Leu Ala Val Glu 1 5 10 15 His Val Lys Gly Asp Val Ser Val Ser Val Glu Glu Gly Lys Glu Asn 20 25 30 Leu Leu Arg Val Ser Glu Asp Val Val Phe Thr Asp Ile Asn Ser Ile 35 40 45 Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr Ser Gly Leu Tyr Gly Thr 50 55 60 Asn Leu Met Glu His Thr Glu Ile Asp His Trp Leu Glu Phe Ser Ala 65 70 75 80 Thr Lys Leu Ser Ser Cys Ala Ala Leu Thr Ser Ala Leu Thr Glu Leu 85 90 95 Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn Ser Leu Thr 100 105 110 Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn Ala Ala Trp 115 120 125 Gln Glu Gln Leu Glu Gln Asn Lys Thr Leu Val His Val Lys Arg Trp 130 135 140 Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe Arg Ser Val Gly Thr Lys 145 150 155 160 Trp Asp Val Ser Glu Asn Lys Ala Arg Val Val Pro Asp Lys Lys Gln 165 170 175 Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met Gly Lys Val 180 185 190 Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His Ile Gly His 195 200 205 Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn Phe Lys Gly 210 215 220 Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys Glu Lys Glu 225 230 235 240 Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu His Ile Lys 245 250 255 Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile Met Lys 260 265 270 Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr 275 280 285 Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Ala Glu Ser Lys 290 295 300 His Arg Gln Asn Ser Val Glu Lys Asn Leu Gln Met Trp Glu Glu Met 305 310 315 320 Lys Lys Gly Ser Pro Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile 325 330 335 Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg 340 345 350 Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr 355 360 365 Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val 370 375 380 Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe 385 390 395 400 Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu 405 410 415 Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu 420 425 430 Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg 435 440 445 Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly 450 455 460 Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn 465 470 475 480 Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Val Ile Asp Pro 485 490 495 Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Val Pro Val 500 505 510 Asn Val Pro Glu Ala Gln Glu Glu Met Lys Glu Val Ala Arg His Pro 515 520 525 Lys Asn Pro Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val 530 535 540 Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val 545 550 555 560 Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Asn 565 570 575 Ala Asp Gly Lys Ile Thr Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn 580 585 590 Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr 595 600 605 His Ala Leu Pro Ile Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile 610 615 620 Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn 625 630 635 640 Lys Asp Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys 645 650 655 Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe 660 665 670 Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu 675 680 685 Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met 690 695 700 Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Ala Glu Pro Leu Lys Lys 705 710 715 720 Glu Thr Ser Ser Ala Pro Lys Glu Gly Pro Val Pro Ala Val Ser Pro 725 730 735 Cys Ala Ala Ser Glu Glu Ser Ser Val Leu Tyr Asn Arg Val Ala Ala 740 745 750 Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu 755 760 765 Asp Val Asp Ala Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr 770 775 780 Lys Gln Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ser Ala 785 790 795 800 Ala Ala Gln Ser Ala Ser Thr Lys Ser Leu Pro Ser Ala Gly Glu Asp 805 810 815 Arg Ser Leu Tyr Asp Lys Ile Ala Ala Gln Gly Glu Val Val Arg Lys 820 825 830 Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Val Thr Glu Ala Val Glu 835 840 845 Cys Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Lys Glu 850 855 860 Tyr Val Pro Gly Gln Pro Pro Ala Ser Gln Lys Ser Gln Pro Ser Pro 865 870 875 880 Ala Ser Lys Ala Glu Pro Ala Gly Pro Glu Thr Thr Glu Ala Lys Ala 885 890 895 Leu Phe Asp Arg Val Ala Cys Gln Gly Glu Val Val Arg Lys Leu Lys 900 905 910 Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Pro Ala Val Gln Glu Leu 915 920 925 Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly Ile Glu Tyr Lys 930 935 940 Pro Val Ser Ala Thr Gly Ser Glu Asp Lys Asp Lys Lys Lys Lys Glu 945 950 955 960 Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp 965 970 975 Gly Pro Gly Lys Asp Ser Ser Lys Ser Gln Gly Gly Gly Leu Ser Ser 980 985 990 Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly 995 1000 1005 Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Glu Trp Tyr Ser Gln Val 1010 1015 1020 Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr 1025 1030 1035 1040 Ile Leu Arg Pro Trp Ser Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe 1045 1050 1055 Phe Asp Thr Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro 1060 1065 1070 Ile Phe Val Ser Gln Ala Ala Leu Glu Lys Glu Lys Ser His Ile Glu 1075 1080 1085 Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu 1090 1095 1100 Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr 1105 1110 1115 1120 Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg 1125 1130 1135 Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln 1140 1145 1150 Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala 1155 1160 1165 Phe Ala Thr Phe Glu Glu Ala Ala Asp Glu Val Met Gln Ile Leu Glu 1170 1175 1180 Leu Tyr Ala Arg Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Arg 1185 1190 1195 1200 Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr 1205 1210 1215 Thr Val Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala 1220 1225 1230 Thr Ser His His Leu Gly Gln Asn Phe Ser Lys Met Cys Glu Ile Val 1235 1240 1245 Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Phe Gln Cys 1250 1255 1260 Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Ile Met Val His 1265 1270 1275 1280 Gly Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln 1285 1290 1295 Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp 1300 1305 1310 Arg Glu Ala Leu Met Ala Lys Cys Asn Glu Tyr Arg Lys Arg Leu Leu 1315 1320 1325 Gly Val Asn Ile Arg Val Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro 1330 1335 1340 Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Val Arg Leu 1345 1350 1355 1360 Glu Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg 1365 1370 1375 Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala Glu Lys Glu Ala Glu Ser 1380 1385 1390 Lys Leu Gln Glu Ile Leu Glu Asp Ile Gln Leu Asn Leu Phe Thr Arg 1395 1400 1405 Ala Ser Glu Asp Leu Lys Thr His Met Val Val Ser Asn Thr Leu Glu 1410 1415 1420 Asp Phe Gln Lys Val Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe 1425 1430 1435 1440 Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg 1445 1450 1455 Asp Gln Asp Val Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu 1460 1465 1470 Cys Ile Pro Phe Thr Pro Leu Cys Glu Leu Gln Pro Gly Ala Met Cys 1475 1480 1485 Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser 1490 1495 1500 Tyr 150 <210> 96 <211> 1477 <212> PRT <213> Artificial Sequence <220> <223> EPRS AAH94679.1 <400> 96 Lys Glu Asn Leu Leu Arg Val Ser Glu Thr Val Ala Phe Thr Asp Val 1 5 10 15 Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr Ser Gly Leu 20 25 30 Tyr Gly Thr Asn Leu Met Glu His Thr Glu Ile Asp His Trp Leu Glu 35 40 45 Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Arg Leu Thr Ser Ala Ile 50 55 60 Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn 65 70 75 80 Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Ser 85 90 95 Ala Ala Trp Gln Glu His Leu Lys Gln Asn Lys Thr Leu Val His Val 100 105 110 Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe Arg Ser Val 115 120 125 Gly Thr Lys Trp Asp Val Ser Gly Asn Arg Ala Thr Val Ala Pro Asp 130 135 140 Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met 145 150 155 160 Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His 165 170 175 Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn 180 185 190 Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys 195 200 205 Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu 210 215 220 His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr 225 230 235 240 Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val 245 250 255 Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Thr 260 265 270 Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu Gln Met Trp 275 280 285 Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu Arg 290 295 300 Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr 305 310 315 320 Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr 325 330 335 Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile 340 345 350 Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp 355 360 365 Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr 370 375 380 Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys 385 390 395 400 Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp 405 410 415 Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr 420 425 430 Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser 435 440 445 Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Val 450 455 460 Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val 465 470 475 480 Val Pro Val Asn Val Leu Asp Ala Gln Glu Glu Met Lys Glu Val Ala 485 490 495 Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val Trp Tyr Ser 500 505 510 Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly 515 520 525 Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile 530 535 540 His Lys Asn Ala Asp Gly Lys Ile Thr Ser Leu Asp Ala Lys Leu Asn 545 550 555 560 Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala 565 570 575 Glu Ser Thr His Ala Leu Ser Ile Pro Ala Val Cys Val Thr Tyr Glu 580 585 590 His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys Gln 595 600 605 Tyr Ile Asn Lys Asp Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro 610 615 620 Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg 625 630 635 640 Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser 645 650 655 Cys Arg Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp Gly His Thr 660 665 670 Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Val Glu Ile 675 680 685 Ser Lys Lys Glu Thr Ser Ser Ala Pro Lys Glu Arg Pro Ala Pro Ala 690 695 700 Val Ser Ser Thr Cys Ala Thr Ala Glu Asp Ser Ser Val Leu Tyr Ser 705 710 715 720 Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys 725 730 735 Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu Thr Leu 740 745 750 Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn 755 760 765 Pro Ser Ala Ala Ala Val Gln Thr Val Ser Thr Lys Ser Ser Ser Asn 770 775 780 Thr Val Glu Ser Thr Ser Leu Tyr Asn Lys Val Ala Ala Gln Gly Glu 785 790 795 800 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Val Thr 805 810 815 Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys 820 825 830 Thr Gly Lys Asp Tyr Val Pro Gly Gln Pro Pro Ala Ser Gln Asn Ser 835 840 845 His Ser Asn Pro Val Ser Asn Ala Gln Pro Ala Gly Ala Glu Lys Pro 850 855 860 Glu Ala Lys Val Leu Phe Asp Arg Val Ala Cys Gln Gly Glu Val Val 865 870 875 880 Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Ser Ala 885 890 895 Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly 900 905 910 Ile Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys 915 920 925 Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln 930 935 940 Lys Gln Asn Asp Gly Gln Gly Lys Asp Ser Ser Lys Ser Gln Gly Ser 945 950 955 960 Gly Leu Ser Ser Gly Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln 965 970 975 Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Glu Trp 980 985 990 Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val 995 1000 1005 Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ser Tyr Ser Ile Trp Glu Ser 1010 1015 1020 Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn 1025 1030 1035 1040 Cys Tyr Phe Pro Ile Phe Val Ser Gln Ala Ala Leu Glu Lys Glu Lys 1045 1050 1055 Asn His Ile Glu Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser 1060 1065 1070 Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu 1075 1080 1085 Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp 1090 1095 1100 Leu Pro Val Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe 1105 1110 1115 1120 Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu 1125 1130 1135 Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Asp Glu Val Leu 1140 1145 1150 Gln Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu Leu Leu Ala Ile 1155 1160 1165 Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly 1170 1175 1180 Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala 1185 1190 1195 1200 Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser Lys Met 1205 1210 1215 Cys Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe 1220 1225 1230 Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met 1235 1240 1245 Val Met Val His Gly Asp Ser Met Gly Leu Val Leu Pro Pro Arg Val 1250 1255 1260 Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn Ala Leu 1265 1270 1275 1280 Ser Glu Glu Asp Arg Glu Ala Leu Met Ala Lys Cys Asn Glu Tyr Arg 1285 1290 1295 Arg Arg Leu Leu Gly Ala Asn Ile Arg Val Arg Val Asp Leu Arg Asp 1300 1305 1310 Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val 1315 1320 1325 Pro Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe 1330 1335 1340 Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala Glu Lys 1345 1350 1355 1360 Glu Ala Glu Ala Lys Leu Glu Lys Val Leu Glu Asp Ile Gln Leu Asn 1365 1370 1375 Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val Val Ser 1380 1385 1390 Asn Thr Leu Glu Asp Phe Gln Lys Val Leu Asp Ala Gly Lys Val Ala 1395 1400 1405 Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys 1410 1415 1420 Met Thr Ala Arg Asp Gln Asp Val Glu Pro Gly Ala Pro Ser Met Gly 1425 1430 1435 1440 Ala Lys Ser Leu Cys Ile Pro Phe Asn Pro Leu Cys Glu Leu Gln Pro 1445 1450 1455 Gly Ala Met Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu 1460 1465 1470 Phe Gly Arg Ser Tyr 1475 <210> 97 <211> 1468 <212> PRT <213> Artificial Sequence <220> <223> EPRS EDL13067.1 <400> 97 Thr Val Ala Phe Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg 1 5 10 15 Ile Ala Thr Thr Pro Gly Leu Tyr Gly Thr Asn Leu Met Glu His Thr 20 25 30 Glu Ile Asp His Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys 35 40 45 Asp Gly Leu Thr Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu 50 55 60 Arg Thr Tyr Leu Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val 65 70 75 80 Trp Ala Thr Leu Lys Gly Ser Ala Ala Trp Gln Glu His Leu Lys Gln 85 90 95 Asn Lys Thr Leu Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala 100 105 110 Gln Gln Ala Phe Arg Ser Val Gly Thr Lys Trp Asp Val Ser Gly Ser 115 120 125 Arg Ala Thr Val Ala Pro Asp Lys Lys Gln Asp Val Gly Lys Phe Val 130 135 140 Glu Leu Pro Gly Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro 145 150 155 160 Glu Ala Ser Gly Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu 165 170 175 Asn Gln His Tyr Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe 180 185 190 Asp Asp Thr Asn Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile 195 200 205 Leu Glu Asp Val Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr 210 215 220 Thr Ser Asp His Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile 225 230 235 240 Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys 245 250 255 Ala Glu Arg Glu Gln Arg Thr Glu Ser Lys His Arg Lys Asn Ser Val 260 265 270 Glu Lys Asn Leu Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe 275 280 285 Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn 290 295 300 Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His 305 310 315 320 Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala 325 330 335 Cys Pro Ile Val Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr 340 345 350 Thr Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala 355 360 365 Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu 370 375 380 Asn Asn Thr Val Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu 385 390 395 400 Gly Leu Val Asp Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly 405 410 415 Val Leu Arg Arg Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala 420 425 430 Ala Gln Gly Ser Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile 435 440 445 Trp Ala Phe Asn Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val 450 455 460 Ala Leu Leu Lys Lys Glu Val Val Pro Val Asn Val Leu Asp Ala Gln 465 470 475 480 Glu Glu Met Lys Glu Val Ala Arg His Pro Lys Asn Pro Asp Val Gly 485 490 495 Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp 500 505 510 Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly 515 520 525 Asn Ile Asn Ile Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Thr 530 535 540 Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr 545 550 555 560 Thr Lys Ile Thr Trp Leu Ala Glu Ser Thr His Ala Leu Pro Ile Pro 565 570 575 Ala Val Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly 580 585 590 Lys Asp Glu Asp Phe Lys Gln Tyr Ile Asn Lys Asp Ser Lys His Glu 595 600 605 Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp 610 615 620 Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr 625 630 635 640 Glu Pro Val Ser Pro Tyr Ser Cys Arg Glu Ala Pro Cys Ile Leu Ile 645 650 655 Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys 660 665 670 Glu Lys Thr Lys Val Glu Ile Ser Lys Lys Glu Thr Ser Ser Ala Pro 675 680 685 Lys Glu Arg Pro Ala Pro Ala Val Ser Ser Thr Cys Ala Thr Ala Glu 690 695 700 Asp Ser Ser Val Leu Tyr Ser Arg Val Ala Val Gln Gly Asp Val Val 705 710 715 720 Arg Glu Leu Lys Ala Lys Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala 725 730 735 Val Lys Gln Leu Leu Thr Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly 740 745 750 Gln Glu Tyr Lys Pro Gly Asn Pro Ser Ala Ala Ala Val Gln Thr Val 755 760 765 Ser Thr Lys Ser Ser Ser Asn Thr Val Glu Ser Thr Ser Leu Tyr Asn 770 775 780 Lys Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys 785 790 795 800 Ala Pro Lys Ala Lys Val Thr Glu Ala Val Glu Cys Leu Leu Ser Leu 805 810 815 Lys Ala Glu Tyr Lys Glu Lys Thr Gly Lys Asp Tyr Val Pro Gly Gln 820 825 830 Pro Pro Ala Ser Gln Asn Ser His Ser Asn Pro Val Ser Asn Ala Gln 835 840 845 Pro Ala Gly Ala Glu Lys Pro Glu Ala Lys Val Leu Phe Asp Arg Val 850 855 860 Ala Cys Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser 865 870 875 880 Lys Asp Gln Val Asp Ser Ala Val Gln Glu Leu Leu Gln Leu Lys Ala 885 890 895 Gln Tyr Lys Ser Leu Thr Gly Ile Glu Tyr Lys Pro Val Ser Ala Thr 900 905 910 Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser 915 920 925 Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Gln Gly Lys Asp 930 935 940 Ser Ser Lys Ser Gln Gly Ser Gly Leu Ser Ser Gly Gly Ala Gly Glu 945 950 955 960 Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys 965 970 975 Glu Glu Asn Leu Ala Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu 980 985 990 Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp 995 1000 1005 Ser Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile 1010 1015 1020 Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln 1025 1030 1035 1040 Ala Ala Leu Glu Lys Glu Lys Asn His Ile Glu Asp Phe Ala Pro Glu 1045 1050 1055 Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile 1060 1065 1070 Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys 1075 1080 1085 Trp Val Gln Ser His Arg Asp Leu Pro Val Arg Leu Asn Gln Trp Cys 1090 1095 1100 Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr 1105 1110 1115 1120 Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu 1125 1130 1135 Glu Ala Ala Asp Glu Val Leu Gln Ile Leu Glu Leu Tyr Ala Arg Val 1140 1145 1150 Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu 1155 1160 1165 Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe 1170 1175 1180 Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu 1185 1190 1195 1200 Gly Gln Asn Phe Ser Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys 1205 1210 1215 Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr 1220 1225 1230 Thr Arg Thr Ile Gly Val Met Val Met Val His Gly Asp Asn Met Gly 1235 1240 1245 Leu Val Leu Pro Pro Arg Val Ala Ser Val Gln Val Val Val Ile Pro 1250 1255 1260 Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg Glu Ala Leu Met 1265 1270 1275 1280 Ala Lys Cys Asn Glu Tyr Arg Arg Arg Leu Leu Gly Ala Asn Ile Arg 1285 1290 1295 Val Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn 1300 1305 1310 His Trp Glu Leu Lys Gly Val Pro Val Arg Leu Glu Val Gly Pro Arg 1315 1320 1325 Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu 1330 1335 1340 Lys Leu Thr Ile Ala Glu Lys Glu Ala Glu Ala Lys Leu Glu Lys Val 1345 1350 1355 1360 Leu Glu Asp Ile Gln Leu Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu 1365 1370 1375 Lys Thr His Met Val Val Ser Asn Thr Leu Glu Asp Phe Gln Lys Val 1380 1385 1390 Leu Asp Ala Gly Lys Val Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp 1395 1400 1405 Cys Glu Asp Trp Ile Lys Lys Met Thr Ala Arg Asp Gln Asp Val Glu 1410 1415 1420 Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Asn 1425 1430 1435 1440 Pro Leu Cys Glu Leu Gln Pro Gly Ala Met Cys Val Cys Gly Lys Asn 1445 1450 1455 Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1460 1465 <210> 98 <211> 1447 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_008825022.1 <400> 98 Met Glu His Thr Glu Ile Asp His Trp Leu Glu Phe Ser Ala Thr Lys 1 5 10 15 Leu Ser Ser Cys Asp Leu Phe Thr Ser Ala Val Asp Glu Leu Asn His 20 25 30 Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn Ser Leu Thr Leu Ala 35 40 45 Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn Ala Val Trp Gln Glu 50 55 60 Gln Leu Lys Gln Asn Lys Thr Pro Ile His Val Lys Arg Trp Phe Ser 65 70 75 80 Phe Leu Glu Ala His Gln Ala Phe Arg Ser Val Gly Ala Lys Trp Asp 85 90 95 Val Ser Ala Asn Lys Ala Arg Val Val Pro Asp Lys Lys Gln Asp Ile 100 105 110 Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met Gly Lys Val Thr Val 115 120 125 Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His Ile Gly His Ala Lys 130 135 140 Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn Phe Lys Gly Lys Leu 145 150 155 160 Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys Glu Lys Glu Asp Phe 165 170 175 Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu His Ile Lys Pro Asp 180 185 190 Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile Met Lys Tyr Ala 195 200 205 Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala 210 215 220 Glu Gln Met Lys Val Glu Arg Glu Gln Arg Thr Glu Ser Arg His Arg 225 230 235 240 Glu Asn Ser Val Glu Arg Asn Leu Gln Met Trp Glu Glu Met Lys Lys 245 250 255 Gly Ser Gln Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile Asp Met 260 265 270 Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys 275 280 285 Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr Pro Thr 290 295 300 Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val Thr His 305 310 315 320 Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp 325 330 335 Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser 340 345 350 Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu Thr Trp 355 360 365 Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg Phe Pro 370 375 380 Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly Leu Lys 385 390 395 400 Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn Met Glu 405 410 415 Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Leu Arg Ala Leu Cys Lys 420 425 430 Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys 435 440 445 Glu Val Ile Pro Val Tyr Val Pro Glu Ala Arg Glu Glu Met Lys Glu 450 455 460 Val Ala Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val Trp 465 470 475 480 Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe Ser 485 490 495 Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile Thr 500 505 510 Lys Ile His Lys Asn Ala Asp Gly Lys Ile Leu Ser Leu Asp Ala Lys 515 520 525 Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp 530 535 540 Leu Ala Glu Thr Ser His Thr Pro Pro Val Pro Ala Val Cys Val Thr 545 550 555 560 Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe 565 570 575 Lys Gln Tyr Val Asn Arg Asp Ser Lys His Glu Glu Leu Met Leu Gly 580 585 590 Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln 595 600 605 Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro 610 615 620 Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp Gly 625 630 635 640 His Thr Lys Glu Met Pro Lys Ser Gly Ser Lys Glu Lys Thr Lys Val 645 650 655 Glu Thr Thr Arg Lys Glu Thr Leu Ser Pro Leu Lys Glu Lys Leu Ala 660 665 670 Ala Ser Leu Asn Asn Ser Cys Ala Thr Thr Glu Asp Ser Ser Val Leu 675 680 685 Tyr Lys Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys Ala 690 695 700 Lys Lys Ala Ala Lys Glu Asp Val Asp Ala Ala Val Lys Gln Leu Leu 705 710 715 720 Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys Pro 725 730 735 Gly Asn Pro Pro Ala Ala Val Leu Gln Asn Val Ser Thr Lys Ser Ser 740 745 750 Ser Ala Val Glu Asn Thr Ser Leu Tyr Asp Lys Val Ala Ala Gln Gly 755 760 765 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Val 770 775 780 Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Lys Asp Tyr Val Pro Gly Gln Pro Pro Ser Ser Gln Ser 805 810 815 Ser Asn Pro Ser Leu Gly Ser Asn Ala Asp Pro Ala Gly Pro Glu Thr 820 825 830 Pro Glu Ala Lys Val Leu Phe Asp Gln Val Ala Cys Gln Gly Glu Val 835 840 845 Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Ala 850 855 860 Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr 865 870 875 880 Gly Val Glu Tyr Lys Pro Val Thr Ala Thr Gly Ala Glu Asp Lys Asp 885 890 895 Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro 900 905 910 Gln Lys Gln Asn Asp Gly Gln Gly Lys Asp Ser Ser Arg Asn Gln Gly 915 920 925 Ser Gly Gly Leu Ser Pro Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys 930 935 940 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 945 950 955 960 Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr 965 970 975 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ser Tyr Ser Ile Trp 980 985 990 Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 995 1000 1005 Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Gly Ala Leu Glu Lys 1010 1015 1020 Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1025 1030 1035 1040 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1045 1050 1055 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1060 1065 1070 Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1075 1080 1085 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1090 1095 1100 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1105 1110 1115 1120 Val Leu Gln Ile Leu Glu Leu Tyr Ala Gln Val Tyr Glu Glu Leu Leu 1125 1130 1135 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1140 1145 1150 Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser Gly 1155 1160 1165 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1170 1175 1180 Lys Met Cys Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1185 1190 1195 1200 Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1205 1210 1215 Val Met Ile Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1220 1225 1230 Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1235 1240 1245 Ala Leu Ser Glu Asp Asp Arg Glu Ala Leu Met Ala Lys Cys Ser Glu 1250 1255 1260 Tyr Gln Arg Arg Leu Leu Ser Ala Asn Ile Arg Val Arg Val Asp Leu 1265 1270 1275 1280 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1285 1290 1295 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Thr Cys 1300 1305 1310 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala 1315 1320 1325 Glu Lys Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile Gln 1330 1335 1340 Leu Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Arg Thr His Met Val 1345 1350 1355 1360 Val Ala Asn Thr Leu Glu Asp Phe Gln Lys Ala Leu Asp Ser Gly Lys 1365 1370 1375 Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1380 1385 1390 Lys Lys Thr Thr Ala Arg Asp Gln Asp Val Glu Pro Gly Ala Pro Ser 1395 1400 1405 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys Glu Leu 1410 1415 1420 Gln Pro Gly Ala Lys Cys Ile Cys Gly Lys Asn Pro Ala Lys Phe Tyr 1425 1430 1435 1440 Thr Leu Phe Gly Arg Ser Tyr 1445 <210> 99 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005348894.1 <400> 99 Met Ala Ala Leu Cys Leu Thr Val Asn Ala Gly Asp Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Leu Leu Arg Val Ser Glu Ser Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Ala Thr 50 55 60 Ser Gly Leu Tyr Gly Thr Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Ala Ala Leu Thr 85 90 95 Ser Ala Leu Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Ala Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Val Glu Gln Asn Lys Thr Leu 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Thr Lys Trp Asp Phe Ser Glu Asn Lys Ala Arg Val 165 170 175 Ala Pro Asp Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Met Glu Arg Glu 290 295 300 Gln Arg Ala Glu Ser Lys His Arg Gln Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val His Val Pro Asp Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Thr Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ser His Ala Leu Pro Ile Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asp Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Gln Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Ser Lys Lys Glu Thr Ser Ser Ala Pro Lys Glu Lys Thr 725 730 735 Val Pro Ala Val Ser Ser Thr Ser Ala Ala Pro Glu Asp Ser Ala Val 740 745 750 Leu Tyr Asn Arg Val Ala Leu Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Gln Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Cys Ile Ala Ala Val Gln Ser Val Ser Thr Lys Ser 805 810 815 Pro Ser Ser Thr Val Glu Asn Pro Ser Leu Tyr Asp Lys Val Ala Ala 820 825 830 Gln Gly Glu Ile Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Met Ser 865 870 875 880 Gln Lys Ser Gln Ser Ser Ala Val Ser Gly Ala Glu Pro Ala Gly Pro 885 890 895 Glu Thr Pro Glu Ala Lys Gly Leu Phe Asp Arg Val Ala Arg Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Ser Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Val Glu Tyr Lys Pro Val Ser Ala Ala Gly Ser Glu Asp 945 950 955 960 Arg Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Pro Gly Lys Asp Ser Ser Lys Ser 980 985 990 Gln Gly Asn Gly Leu Ser Pro Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ser Tyr Ser Ile 1045 1050 1055 Trp Glu Phe Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ala Ala Leu Glu 1075 1080 1085 Lys Glu Lys Asn His Ile Glu Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Val Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Asp 1170 1175 1180 Glu Val Leu Gln Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Leu Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Ile Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Phe Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Val Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Arg Glu Ala Leu Met Ala Lys Cys Asn 1315 1320 1325 Glu Tyr Arg Arg Arg Leu Leu Gly Ala Asn Ile Arg Val Arg Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Ser Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile 1380 1385 1390 Ala Glu Lys Glu Ala Glu Ala Lys Leu Gln Glu Ile Leu Glu Asp Ile 1395 1400 1405 Gln Leu Asn Leu Phe Thr Arg Ala Thr Glu Asp Leu Lys Ala His Met 1410 1415 1420 Val Val Ser Asn Thr Leu Glu Asp Phe Gln Lys Ala Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Val Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Asn Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Met Cys Ile Cys Gly Lys Lys Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 100 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS AAI41050.1 <400> 100 Met Ala Ala Leu Cys Leu Thr Val Asn Ala Gly Asn Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Leu Leu Arg Val Ser Glu Thr Val Ala Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ser Gly Leu Tyr Gly Thr Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Gly Leu Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Ser Ala Ala Trp Gln Glu His Leu Lys Gln Asn Lys Thr Leu 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Thr Lys Trp Asp Val Ser Gly Ser Arg Ala Thr Val 165 170 175 Ala Pro Asp Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Thr Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Asn Val Leu Asp Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Thr Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Ser Thr His Ala Leu Pro Ile Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Ile Asn Lys Asp Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Arg Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ile Ser Lys Lys Glu Thr Ser Ser Ala Pro Lys Glu Arg Pro 725 730 735 Ala Pro Ala Val Ser Ser Thr Cys Ala Thr Ala Glu Asp Ser Ser Val 740 745 750 Leu Tyr Ser Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Thr Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Ser Ala Ala Ala Val Gln Thr Val Ser Thr Lys Ser 805 810 815 Ser Ser Asn Thr Val Glu Ser Thr Ser Leu Tyr Asn Lys Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Thr Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Asp Tyr Val Pro Gly Gln Pro Pro Ala Ser 865 870 875 880 Gln Asn Ser His Ser Asn Pro Val Ser Asn Ala Gln Pro Ala Gly Ala 885 890 895 Glu Lys Pro Glu Ala Lys Val Leu Phe Asp Arg Val Ala Cys Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Ser Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Ile Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Gln Gly Lys Asp Ser Ser Lys Ser 980 985 990 Gln Gly Ser Gly Leu Ser Ser Gly Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ser Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ala Ala Leu Glu 1075 1080 1085 Lys Glu Lys Asn His Ile Glu Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Val Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Asp 1170 1175 1180 Glu Val Leu Gln Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Val Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Arg Glu Ala Leu Met Ala Lys Cys Asn 1315 1320 1325 Glu Tyr Arg Arg Arg Leu Leu Gly Ala Asn Ile Arg Val Arg Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile 1380 1385 1390 Ala Glu Lys Glu Ala Glu Ala Lys Leu Glu Lys Val Leu Glu Asp Ile 1395 1400 1405 Gln Leu Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ser Asn Thr Leu Glu Asp Phe Gln Lys Val Leu Asp Ala Gly 1425 1430 1435 1440 Lys Val Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Met Thr Ala Arg Asp Gln Asp Val Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Asn Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Met Cys Val Cys Gly Lys Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 101 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS NP_084011.1 <400> 101 Met Ala Ala Leu Cys Leu Thr Val Asn Ala Gly Asn Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Leu Leu Arg Val Ser Glu Thr Val Ala Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ser Gly Leu Tyr Gly Thr Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Arg Leu Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Ser Ala Ala Trp Gln Glu His Leu Lys Gln Asn Lys Thr Leu 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Thr Lys Trp Asp Val Ser Gly Asn Arg Ala Thr Val 165 170 175 Ala Pro Asp Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Thr Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Asn Val Leu Asp Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Thr Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Ser Thr His Ala Leu Ser Ile Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Ile Asn Lys Asp Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Arg Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ile Ser Lys Lys Glu Thr Ser Ser Ala Pro Lys Glu Arg Pro 725 730 735 Ala Pro Ala Val Ser Ser Thr Cys Ala Thr Ala Glu Asp Ser Ser Val 740 745 750 Leu Tyr Ser Arg Val Ala Val Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Thr Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Ser Ala Ala Ala Val Gln Thr Val Ser Thr Lys Ser 805 810 815 Ser Ser Asn Thr Val Glu Ser Thr Ser Leu Tyr Asn Lys Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Thr Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Asp Tyr Val Pro Gly Gln Pro Pro Ala Ser 865 870 875 880 Gln Asn Ser His Ser Asn Pro Val Ser Asn Ala Gln Pro Ala Gly Ala 885 890 895 Glu Lys Pro Glu Ala Lys Val Leu Phe Asp Arg Val Ala Cys Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Ser Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Ile Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Gln Gly Lys Asp Ser Ser Lys Ser 980 985 990 Gln Gly Ser Gly Leu Ser Ser Gly Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ser Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ala Ala Leu Glu 1075 1080 1085 Lys Glu Lys Asn His Ile Glu Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Val Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Asp 1170 1175 1180 Glu Val Leu Gln Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Ile Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Val Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Arg Glu Ala Leu Met Ala Lys Cys Asn 1315 1320 1325 Glu Tyr Arg Arg Arg Leu Leu Gly Ala Asn Ile Arg Val Arg Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile 1380 1385 1390 Ala Glu Lys Glu Ala Glu Ala Lys Leu Glu Lys Val Leu Glu Asp Ile 1395 1400 1405 Gln Leu Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ser Asn Thr Leu Glu Asp Phe Gln Lys Val Leu Asp Ala Gly 1425 1430 1435 1440 Lys Val Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Met Thr Ala Arg Asp Gln Asp Val Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Asn Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Met Cys Val Cys Gly Lys Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 102 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006497180.1 <400> 102 Met Ala Ala Leu Cys Leu Thr Val Asn Ala Gly Asn Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Leu Leu Arg Val Ser Glu Thr Val Ala Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ile Ala Thr Thr 50 55 60 Ser Gly Leu Tyr Gly Thr Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Arg Leu Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Ser Ala Ala Trp Gln Glu His Leu Lys Gln Asn Lys Thr Leu 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Thr Lys Trp Asp Val Ser Gly Asn Arg Ala Thr Val 165 170 175 Ala Pro Asp Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Thr Glu Ser Lys His Arg Lys Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Val Pro Val Asn Val Leu 515 520 525 Asp Ala Gln Glu Glu Met Lys Glu Val Ala Arg His Pro Lys Asn Pro 530 535 540 Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Ile Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Asn Ala Asp Gly 580 585 590 Lys Ile Thr Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Ser Thr His Ala Leu 610 615 620 Ser Ile Pro Ala Val Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Ile Asn Lys Asp Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Arg Glu Ala Pro Cys 690 695 700 Ile Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Ile Ser Lys Lys Glu Thr Ser 725 730 735 Ser Ala Pro Lys Glu Arg Pro Ala Pro Ala Val Ser Ser Thr Cys Ala 740 745 750 Thr Ala Glu Asp Ser Ser Val Leu Tyr Ser Arg Val Ala Val Gln Gly 755 760 765 Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Pro Lys Glu Asp Ile 770 775 780 Asp Ala Ala Val Lys Gln Leu Leu Thr Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Ser Ala Ala Ala Val 805 810 815 Gln Thr Val Ser Thr Lys Ser Ser Ser Asn Thr Val Glu Ser Thr Ser 820 825 830 Leu Tyr Asn Lys Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys 835 840 845 Ala Glu Lys Ala Pro Lys Ala Lys Val Thr Glu Ala Val Glu Cys Leu 850 855 860 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Lys Asp Tyr Val 865 870 875 880 Pro Gly Gln Pro Pro Ala Ser Gln Asn Ser His Ser Asn Pro Val Ser 885 890 895 Asn Ala Gln Pro Ala Gly Ala Glu Lys Pro Glu Ala Lys Val Leu Phe 900 905 910 Asp Arg Val Ala Cys Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu 915 920 925 Lys Ala Ser Lys Asp Gln Val Asp Ser Ala Val Gln Glu Leu Leu Gln 930 935 940 Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly Ile Glu Tyr Lys Pro Val 945 950 955 960 Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu 965 970 975 Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Gln 980 985 990 Gly Lys Asp Ser Ser Lys Ser Gln Gly Ser Gly Leu Ser Ser Gly Gly 995 1000 1005 Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Ala Lys Lys Glu Glu Asn Leu Ala Glu Trp Tyr Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Ser Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp 1060 1065 1070 Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Ile Phe 1075 1080 1085 Val Ser Gln Ala Ala Leu Glu Lys Glu Lys Asn His Ile Glu Asp Phe 1090 1095 1100 Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala 1105 1110 1115 1120 Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala 1125 1130 1135 Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Val Arg Leu Asn 1140 1145 1150 Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe 1155 1160 1165 Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala 1170 1175 1180 Thr Phe Glu Glu Ala Ala Asp Glu Val Leu Gln Ile Leu Glu Leu Tyr 1185 1190 1195 1200 Ala Arg Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Arg Gly Arg 1205 1210 1215 Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Ile 1220 1225 1230 Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser 1235 1240 1245 His His Leu Gly Gln Asn Phe Ser Lys Met Cys Glu Ile Val Phe Glu 1250 1255 1260 Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Cys Ser Trp 1265 1270 1275 1280 Gly Leu Thr Thr Arg Thr Ile Gly Val Met Val Met Val His Gly Asp 1285 1290 1295 Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Ser Val Gln Val Val 1300 1305 1310 Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg Glu 1315 1320 1325 Ala Leu Met Ala Lys Cys Asn Glu Tyr Arg Arg Arg Leu Leu Gly Ala 1330 1335 1340 Asn Ile Arg Val Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp 1345 1350 1355 1360 Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Val Arg Leu Glu Val 1365 1370 1375 Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp 1380 1385 1390 Thr Gly Glu Lys Leu Thr Ile Ala Glu Lys Glu Ala Glu Ala Lys Leu 1395 1400 1405 Glu Lys Val Leu Glu Asp Ile Gln Leu Asn Leu Phe Thr Arg Ala Ser 1410 1415 1420 Glu Asp Leu Lys Thr His Met Val Val Ser Asn Thr Leu Glu Asp Phe 1425 1430 1435 1440 Gln Lys Val Leu Asp Ala Gly Lys Val Ala Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Met Thr Ala Arg Asp Gln 1460 1465 1470 Asp Val Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Asn Pro Leu Cys Glu Leu Gln Pro Gly Ala Met Cys Val Cys 1490 1495 1500 Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 103 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005082493.1 <400> 103 Met Ala Ala Leu Cys Leu Thr Val Asn Ala Gly Asp Pro Pro Leu Asp 1 5 10 15 Ala Leu Leu Ala Val Glu His Leu Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Leu Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Leu Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ser Gly Leu Tyr Gly Thr Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Ala Ala Leu Thr 85 90 95 Ser Ala Leu Thr Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Gln Leu Glu Gln Asn Arg Thr Leu 130 135 140 Val His Val Arg Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Ala Lys Trp Asn Val Ser Glu Asn Lys Ala Arg Val 165 170 175 Ala Pro Asp Lys Lys Gln Asp Val Gly Lys Phe Ile Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Glu Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Thr Glu Ser Lys His Arg Gln Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Pro Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Asn Val Pro Asp Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Ala Asp Gly Lys Ile Ile Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala His Ala Leu Pro Ile Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Ser Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asp Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Ala Glu Pro Ser Lys Lys Glu Thr Ser Ser Ala Pro Lys Glu Gly Pro 725 730 735 Met Pro Ala Val Ser Ile Cys Ala Ala Ser Glu Ser Ser Ser Thr Leu 740 745 750 Tyr Ser Arg Val Ala Ala Gln Gly Asp Val Val Arg Asp Leu Lys Ala 755 760 765 Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu Leu 770 775 780 Ala Leu Lys Ala Glu Tyr Lys Gln Lys Thr Gly His Glu Tyr Lys Pro 785 790 795 800 Gly Asn Pro Pro Thr Ala Pro Ala Gln Ser Ala Ser Thr Glu Pro Pro 805 810 815 Pro Ser Ala Val Glu Asp Gly Ser Leu Tyr Asp Lys Val Ala Ala Gln 820 825 830 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys 835 840 845 Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr Lys 850 855 860 Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ala Pro Gln 865 870 875 880 Arg Ser Gln Pro Ser Pro Ala Arg Thr Ala Glu Pro Ala Gly Ala Glu 885 890 895 Thr Ala Glu Ala Arg Ala Leu Phe Asp Arg Val Ala Cys Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Gly Gln Val Asp 915 920 925 Ser Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser Leu 930 935 940 Thr Gly Ile Glu Tyr Lys Pro Val Ser Ala Thr Gly Ser Glu Asp Lys 945 950 955 960 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Gln Asn Asp Gly Gln Gly Lys Asp Ser Ser Lys Ser Gln 980 985 990 Gly Ser Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly Gln Gly Pro Lys 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 1010 1015 1020 Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ser Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ala Ala Leu Glu Lys 1075 1080 1085 Glu Lys Ser His Ile Glu Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Asp Glu 1170 1175 1180 Val Leu Gln Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1250 1255 1260 Gln Phe Ala Phe Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Ile Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Glu Asp Arg Glu Ala Leu Met Ala Lys Cys Asn Glu 1315 1320 1325 Tyr Gln Arg Arg Leu Leu Gly Ala Asn Ile Arg Val Arg Val Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala 1380 1385 1390 Glu Lys Glu Ala Glu Thr Lys Leu Gln Glu Val Leu Glu Asp Ile Gln 1395 1400 1405 Leu Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ser Asn Thr Leu Glu Asp Phe Gln Lys Val Leu Asp Ser Gly Lys 1425 1430 1435 1440 Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Ala Arg Asp Gln Asp Met Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Asn Pro Leu Cys Glu Leu 1475 1480 1485 Gln Pro Gly Ala Met Cys Val Cys Gly Lys Asn Ala Ala Lys Tyr Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 104 <211> 1520 <212> PRT <213> Artificial Sequence <220> <223> EPRS ERE73006.1 <400> 104 Met Ala Leu Thr Arg Ser Leu Phe Leu Tyr Ala Leu Leu Ala Val Glu 1 5 10 15 His Val Lys Gly Asp Val Ser Val Ser Val Glu Glu Gly Lys Glu Asn 20 25 30 Leu Leu Arg Val Ser Glu Asp Val Val Phe Thr Asp Ile Asn Ser Ile 35 40 45 Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr Ser Gly Leu Tyr Gly Thr 50 55 60 Asn Leu Met Glu His Thr Glu Ile Asp His Trp Leu Glu Phe Ser Ala 65 70 75 80 Thr Lys Leu Ser Ser Cys Ala Ala Leu Thr Ser Ala Leu Thr Glu Leu 85 90 95 Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn Ser Leu Thr 100 105 110 Leu Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn Ala Ala Trp 115 120 125 Gln Glu Gln Leu Glu Gln Asn Lys Thr Leu Val His Val Lys Arg Trp 130 135 140 Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe Arg Ser Val Gly Thr Lys 145 150 155 160 Trp Asp Val Ser Glu Asn Lys Ala Arg Val Val Pro Asp Lys Lys Gln 165 170 175 Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met Gly Lys Val 180 185 190 Thr Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His Ile Gly His 195 200 205 Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn Phe Lys Gly 210 215 220 Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys Glu Lys Glu 225 230 235 240 Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu His Ile Lys 245 250 255 Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile Met Lys 260 265 270 Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr 275 280 285 Pro Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Ala Glu Ser Lys 290 295 300 His Arg Gln Asn Ser Val Glu Lys Asn Leu Gln Met Trp Glu Glu Met 305 310 315 320 Lys Lys Gly Ser Pro Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile 325 330 335 Asp Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg 340 345 350 Cys Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr 355 360 365 Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val 370 375 380 Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe 385 390 395 400 Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu 405 410 415 Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu 420 425 430 Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg 435 440 445 Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly 450 455 460 Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn 465 470 475 480 Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Gln Gly Thr Ile 485 490 495 Ile Glu Ala Met His Ser Asp Leu Trp Glu Met Val Ile Asp Pro Val 500 505 510 Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Val Pro Val Asn 515 520 525 Val Pro Glu Ala Gln Glu Glu Met Lys Glu Val Ala Arg His Pro Lys 530 535 540 Asn Pro Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe 545 550 555 560 Ile Glu Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr 565 570 575 Phe Ile Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Asn Ala 580 585 590 Asp Gly Lys Ile Thr Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys 595 600 605 Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr His 610 615 620 Ala Leu Pro Ile Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr 625 630 635 640 Lys Pro Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys 645 650 655 Asp Ser Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp 660 665 670 Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile 675 680 685 Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala 690 695 700 Pro Cys Ile Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro 705 710 715 720 Thr Ser Gly Ser Lys Glu Lys Thr Lys Ala Glu Pro Leu Lys Lys Glu 725 730 735 Thr Ser Ser Ala Pro Lys Glu Gly Pro Val Pro Ala Val Ser Pro Cys 740 745 750 Ala Ala Ser Glu Glu Ser Ser Val Leu Tyr Asn Arg Val Ala Ala Gln 755 760 765 Gly Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp 770 775 780 Val Asp Ala Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr Lys 785 790 795 800 Gln Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Ser Ala Ala 805 810 815 Ala Gln Ser Ala Ser Thr Lys Ser Leu Pro Ser Ala Gly Glu Asp Arg 820 825 830 Ser Leu Tyr Asp Lys Ile Ala Ala Gln Gly Glu Val Val Arg Lys Leu 835 840 845 Lys Ala Glu Lys Ala Pro Lys Ala Lys Val Thr Glu Ala Val Glu Cys 850 855 860 Leu Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Lys Glu Tyr 865 870 875 880 Val Pro Gly Gln Pro Pro Ala Ser Gln Lys Ser Gln Pro Ser Pro Ala 885 890 895 Ser Lys Ala Glu Pro Ala Gly Pro Glu Thr Thr Glu Ala Lys Ala Leu 900 905 910 Phe Asp Arg Val Ala Cys Gln Gly Glu Val Val Arg Lys Leu Lys Ala 915 920 925 Glu Lys Ala Ser Lys Asp Gln Val Asp Pro Ala Val Gln Glu Leu Leu 930 935 940 Gln Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly Ile Glu Tyr Lys Pro 945 950 955 960 Val Ser Ala Thr Gly Ser Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys 965 970 975 Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly 980 985 990 Pro Gly Lys Asp Ser Ser Lys Ser Gln Gly Gly Gly Leu Ser Ser Ser 995 1000 1005 Gly Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu 1010 1015 1020 Glu Ala Lys Lys Glu Glu Asn Leu Ala Glu Trp Tyr Ser Gln Val Ile 1025 1030 1035 1040 Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile 1045 1050 1055 Leu Arg Pro Trp Ser Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe 1060 1065 1070 Asp Thr Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Ile 1075 1080 1085 Phe Val Ser Gln Ala Ala Leu Glu Lys Glu Lys Ser His Ile Glu Asp 1090 1095 1100 Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu 1105 1110 1115 1120 Ala Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro 1125 1130 1135 Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu 1140 1145 1150 Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro 1155 1160 1165 Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe 1170 1175 1180 Ala Thr Phe Glu Glu Ala Ala Asp Glu Val Met Gln Ile Leu Glu Leu 1185 1190 1195 1200 Tyr Ala Arg Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Arg Gly 1205 1210 1215 Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr 1220 1225 1230 Val Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr 1235 1240 1245 Ser His His Leu Gly Gln Asn Phe Ser Lys Met Cys Glu Ile Val Phe 1250 1255 1260 Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Phe Gln Cys Ser 1265 1270 1275 1280 Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Ile Met Val His Gly 1285 1290 1295 Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val 1300 1305 1310 Val Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg 1315 1320 1325 Glu Ala Leu Met Ala Lys Cys Asn Glu Tyr Arg Lys Arg Leu Leu Gly 1330 1335 1340 Val Asn Ile Arg Val Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly 1345 1350 1355 1360 Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Val Arg Leu Glu 1365 1370 1375 Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg 1380 1385 1390 Asp Thr Gly Glu Lys Leu Thr Ile Ala Glu Lys Glu Ala Glu Ser Lys 1395 1400 1405 Leu Gln Glu Ile Leu Glu Asp Ile Gln Leu Asn Leu Phe Thr Arg Ala 1410 1415 1420 Ser Glu Asp Leu Lys Thr His Met Val Val Ser Asn Thr Leu Glu Asp 1425 1430 1435 1440 Phe Gln Lys Val Leu Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys 1445 1450 1455 Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp 1460 1465 1470 Gln Asp Val Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys 1475 1480 1485 Ile Pro Phe Thr Pro Leu Cys Glu Leu Gln Pro Gly Ala Met Cys Val 1490 1495 1500 Cys Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 1520 <210> 105 <211> 1499 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_007636777.1 <400> 105 Val Asn Ala Gly Asp Pro Pro Leu Asp Ala Leu Leu Ala Val Glu His 1 5 10 15 Val Lys Gly Asp Val Ser Val Ser Val Glu Glu Gly Lys Glu Asn Leu 20 25 30 Leu Arg Val Ser Glu Asp Val Val Phe Thr Asp Ile Asn Ser Ile Leu 35 40 45 Arg Tyr Leu Ala Arg Val Ala Thr Thr Ser Gly Leu Tyr Gly Thr Asn 50 55 60 Leu Met Glu His Thr Glu Ile Asp His Trp Leu Glu Phe Ser Ala Thr 65 70 75 80 Lys Leu Ser Ser Cys Ala Ala Leu Thr Ser Ala Leu Thr Glu Leu Asn 85 90 95 His Cys Leu Ser Leu Arg Thr Tyr Leu Val Gly Asn Ser Leu Thr Leu 100 105 110 Ala Asp Leu Cys Val Trp Ala Thr Leu Lys Gly Asn Ala Ala Trp Gln 115 120 125 Glu Gln Leu Glu Gln Asn Lys Thr Leu Val His Val Lys Arg Trp Phe 130 135 140 Gly Phe Leu Glu Ala Gln Gln Ala Phe Arg Ser Val Gly Thr Lys Trp 145 150 155 160 Asp Val Ser Glu Asn Lys Ala Arg Val Val Pro Asp Lys Lys Gln Asp 165 170 175 Val Gly Lys Phe Val Glu Leu Pro Gly Ala Glu Met Gly Lys Val Thr 180 185 190 Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr Leu His Ile Gly His Ala 195 200 205 Lys Ala Ala Leu Leu Asn Gln His Tyr Gln Val Asn Phe Lys Gly Lys 210 215 220 Leu Ile Met Arg Phe Asp Asp Thr Asn Pro Glu Lys Glu Lys Glu Asp 225 230 235 240 Phe Glu Lys Val Ile Leu Glu Asp Val Ala Met Leu His Ile Lys Pro 245 250 255 Asp Gln Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile Met Lys Tyr 260 265 270 Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr Pro 275 280 285 Ala Glu Gln Met Lys Ala Glu Arg Glu Gln Arg Ala Glu Ser Lys His 290 295 300 Arg Gln Asn Ser Val Glu Lys Asn Leu Gln Met Trp Glu Glu Met Lys 305 310 315 320 Lys Gly Ser Pro Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile Asp 325 330 335 Met Ser Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys 340 345 350 Lys Ile Gln Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr Pro 355 360 365 Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val Thr 370 375 380 His Ala Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr 385 390 395 400 Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu Tyr 405 410 415 Ser Arg Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu Thr 420 425 430 Trp Phe Val Asn Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg Phe 435 440 445 Pro Thr Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly Leu 450 455 460 Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn Met 465 470 475 480 Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys Lys Val Ile Asp Pro Val 485 490 495 Ala Pro Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Val Pro Val Asn 500 505 510 Val Pro Glu Ala Gln Glu Glu Met Lys Glu Val Ala Arg His Pro Lys 515 520 525 Asn Pro Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe 530 535 540 Ile Glu Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr 545 550 555 560 Phe Ile Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Asn Ala 565 570 575 Asp Gly Lys Ile Thr Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Thr 580 585 590 Asp Trp Ser Arg Thr Cys Cys Val Ser Lys Thr Gly Leu Asp Leu Pro 595 600 605 Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly 610 615 620 Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asp Ser Lys His Glu 625 630 635 640 Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp 645 650 655 Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr 660 665 670 Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile 675 680 685 Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys 690 695 700 Glu Lys Thr Lys Ala Glu Pro Leu Lys Lys Glu Thr Ser Ser Ala Pro 705 710 715 720 Lys Glu Gly Pro Val Pro Ala Val Ser Pro Cys Ala Ala Ser Glu Glu 725 730 735 Ser Ser Val Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg 740 745 750 Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp Val Asp Ala Ala Val 755 760 765 Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr Lys Gln Lys Thr Gly Gln 770 775 780 Glu Tyr Lys Pro Gly Asn Pro Pro Ser Ala Ala Ala Gln Ser Ala Ser 785 790 795 800 Thr Lys Ser Leu Pro Ser Ala Gly Glu Asp Arg Ser Leu Tyr Asp Lys 805 810 815 Ile Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala 820 825 830 Pro Lys Ala Lys Val Thr Glu Ala Val Glu Cys Leu Leu Ser Leu Lys 835 840 845 Ala Glu Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro 850 855 860 Pro Ala Ser Gln Lys Ser Gln Pro Ser Pro Ala Ser Lys Ala Glu Pro 865 870 875 880 Ala Gly Pro Glu Thr Thr Glu Ala Lys Ala Leu Phe Asp Arg Val Ala 885 890 895 Cys Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys 900 905 910 Asp Gln Val Asp Pro Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln 915 920 925 Tyr Lys Ser Leu Thr Gly Ile Glu Tyr Lys Pro Val Ser Ala Thr Gly 930 935 940 Ser Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu 945 950 955 960 Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Pro Gly Lys Asp Ser 965 970 975 Ser Lys Ser Gln Gly Gly Gly Leu Ser Ser Ser Gly Ala Gly Glu Gly 980 985 990 Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu 995 1000 1005 Glu Asn Leu Ala Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met 1010 1015 1020 Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ser 1025 1030 1035 1040 Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp Thr Glu Ile Lys 1045 1050 1055 Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ala 1060 1065 1070 Ala Leu Glu Lys Glu Lys Ser His Ile Glu Asp Phe Ala Pro Glu Val 1075 1080 1085 Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala 1090 1095 1100 Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp 1105 1110 1115 1120 Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn 1125 1130 1135 Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg 1140 1145 1150 Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu 1155 1160 1165 Ala Ala Asp Glu Val Met Gln Ile Leu Glu Leu Tyr Ala Arg Val Tyr 1170 1175 1180 Glu Glu Leu Leu Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys 1185 1190 1195 1200 Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile 1205 1210 1215 Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly 1220 1225 1230 Gln Asn Phe Ser Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Thr 1235 1240 1245 Pro Gly Glu Lys Gln Phe Ala Phe Gln Cys Ser Trp Gly Leu Thr Thr 1250 1255 1260 Arg Thr Ile Gly Val Met Ile Met Val His Gly Asp Asn Met Gly Leu 1265 1270 1275 1280 Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys 1285 1290 1295 Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg Glu Ala Leu Met Ala 1300 1305 1310 Lys Cys Asn Glu Tyr Arg Lys Arg Leu Leu Gly Val Asn Ile Arg Val 1315 1320 1325 Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His 1330 1335 1340 Trp Glu Leu Lys Gly Val Pro Val Arg Leu Glu Val Gly Pro Arg Asp 1345 1350 1355 1360 Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys 1365 1370 1375 Leu Thr Ile Ala Glu Lys Glu Ala Glu Ser Lys Leu Gln Glu Ile Leu 1380 1385 1390 Glu Asp Ile Gln Leu Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys 1395 1400 1405 Thr His Met Val Val Ser Asn Thr Leu Glu Asp Phe Gln Lys Val Leu 1410 1415 1420 Asp Ser Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys 1425 1430 1435 1440 Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Val Glu Pro 1445 1450 1455 Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Thr Pro 1460 1465 1470 Leu Cys Glu Leu Gln Pro Gly Ala Met Cys Val Cys Gly Lys Asn Pro 1475 1480 1485 Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1490 1495 <210> 106 <211> 1513 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004670165.1 <400> 106 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Leu Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Ala Ala Ala Ser 50 55 60 Val Gly Leu Tyr Gly Met Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Ile Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Val Trp Gln Glu Gln Leu Lys Gln Asn Lys Thr Pro 130 135 140 Val His Ile Lys Arg Trp Phe Gly Phe Leu Asp Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Asn Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Ile Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Ser Phe Arg Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Val Glu Ser Lys His Arg Gln Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Ala Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Arg Glu Val Ile Pro Val Ser Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Gly Ser Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Val Val Thr Phe Ile Asn Trp Gly Asn Ile Thr Ile 565 570 575 Thr Lys Ile His Arg Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Cys His Ala Leu Pro Val Pro Ala Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Ile Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asp Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Arg Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Arg Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Phe Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Thr Thr Lys Lys Glu Pro Ile Ser Pro Arg Lys Glu Arg Pro 725 730 735 Ala Pro Ser Gln Ser Ser Ser Phe Cys Ala Ala Ala Glu Gly Pro Val 740 745 750 Phe Leu Tyr Asn Arg Val Ala Val Gln Gly Asp Ala Val Arg Glu Leu 755 760 765 Lys Ala Lys Lys Ala Ala Lys Glu Glu Val Asp Ala Ala Val Arg Gln 770 775 780 Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr 785 790 795 800 Lys Pro Gly His Pro Pro Ala Ala Glu Val Gln Asn Met Ser Ala Gln 805 810 815 Ala Pro Ala Ser Ala Val Glu Ser Arg Ala Leu His Glu Lys Val Ala 820 825 830 Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys 835 840 845 Ala Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu 850 855 860 Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Ile Pro Gly Gln Pro Pro Ser 865 870 875 880 Ser Gln Gly Leu Lys Ser Gly Phe Asp Ser Arg Ser Asp Pro Ala Gly 885 890 895 Pro Glu Thr Pro Glu Ala Lys Ala Leu Phe Asp Lys Val Ala Cys Gln 900 905 910 Gly Glu Ala Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Ala Gln 915 920 925 Val Gly Ala Ala Ala Gln Glu Leu Leu Arg Leu Glu Ala Gln His Gln 930 935 940 Ala Leu Thr Gly Val Glu His Lys Pro Val Thr Ala Pro Gly Ala Glu 945 950 955 960 Asp Arg Asp Lys Lys Arg Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln 965 970 975 Asn Lys Pro Gln Lys Gln Asn Glu Gly Gln Gly Lys Asp Ser Ser Lys 980 985 990 Ser Gln Gly Ser Gly Leu Ser Pro Ser Gly Ala Gly Glu Gly Gln Gly 995 1000 1005 Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn 1010 1015 1020 Leu Ala Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu 1025 1030 1035 1040 Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser 1045 1050 1055 Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu 1060 1065 1070 Gly Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Gly Ala Leu 1075 1080 1085 Glu Lys Glu Lys Asp His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp 1090 1095 1100 Val Thr Arg Ser Gly Lys Thr Asp Leu Ala Glu Pro Ile Ala Ile Arg 1105 1110 1115 1120 Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln 1125 1130 1135 Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val 1140 1145 1150 Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe 1155 1160 1165 Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala 1170 1175 1180 Glu Glu Val Met Gln Ile Leu Glu Leu Tyr Thr Arg Val Tyr Glu Glu 1185 1190 1195 1200 Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys 1205 1210 1215 Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala 1220 1225 1230 Ser Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn 1235 1240 1245 Phe Ser Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly 1250 1255 1260 Glu Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr 1265 1270 1275 1280 Ile Gly Val Met Ile Met Val His Gly Asp Asn Met Gly Leu Val Leu 1285 1290 1295 Pro Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile 1300 1305 1310 Thr Asn Ala Leu Ser Asp Glu Glu Lys Asn Ala Leu Met Ala Lys Cys 1315 1320 1325 Ser Glu Tyr Gln Arg Arg Leu Leu Ala Val Asn Ile Arg Ala Arg Val 1330 1335 1340 Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu 1345 1350 1355 1360 Leu Lys Gly Val Pro Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys 1365 1370 1375 Ser Cys Gln Phe Val Ala Val Arg Arg Asp Asn Gly Glu Lys Leu Thr 1380 1385 1390 Ile Ala Glu Asn Glu Ala Glu Thr Arg Leu Gln Ala Leu Leu Glu Asp 1395 1400 1405 Ile Gln Leu Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Arg Thr His 1410 1415 1420 Met Val Val Ala Asp Thr Leu Glu Asp Phe Gln Lys Leu Leu Asp Ser 1425 1430 1435 1440 Gly Lys Ile Val Gln Ile Pro Phe Cys Ala Glu Ile Asp Cys Glu Asp 1445 1450 1455 Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala 1460 1465 1470 Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys Pro Leu Cys 1475 1480 1485 Glu Leu Gln Pro Gly Ala Lys Cys Ile Cys Gly Lys Asn Pro Ala Lys 1490 1495 1500 Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 107 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006916292.1 <400> 107 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Val Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Ala Thr 50 55 60 Ala Gly Leu Tyr Gly Ala Asn Leu Met Glu His Ala Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Ala 85 90 95 Ala Ala Ile Arg Glu Leu Ser His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Val Cys Val Trp Ala Ala Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Gly Gln Leu Glu Gln Asn Gln Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Ala Lys Trp Thr Val Ser Ala Thr Lys Gly Lys Ala 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Arg Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Lys His Arg Ser Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Thr Gln Pro His Pro Arg Thr Gly 355 360 365 Ser Lys His Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asp Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Ala Val Leu Val Asn Val Pro Gly Ala Gln Glu Glu Lys Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Arg Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Ser Ile 565 570 575 Thr Lys Ile His Arg Asn Ala Glu Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Arg Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Asp Thr Ala Arg Ala Pro Pro Val Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Arg Asp Glu Asp 625 630 635 640 Phe Lys Gln Phe Val Asn Arg Asp Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Arg Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Arg 705 710 715 720 Val Glu Ser Ala Lys Thr Glu Thr Pro Pro Pro Ala Lys Gly Arg Pro 725 730 735 Ala Pro Pro Ala Thr Gly Ser Arg Ala Pro Ser Glu Asp Pro Leu Val 740 745 750 Leu Tyr Thr Arg Val Ala Ala Gln Gly Asp Ala Val Arg Asp Leu Lys 755 760 765 Ala Gln Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asp Ala Pro Ala Ala Thr Ala Pro Ser Val Pro Pro Ala Ser 805 810 815 Pro Ala Ala Val Pro Gly Ser Gln Ser Leu Tyr Glu Glu Val Ala Ala 820 825 830 Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Val Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Gln Asp Tyr Val Pro His Gln Pro Ala Leu Ser 865 870 875 880 Gln Ser Ser Asp Ser Ser Pro Ala Arg Ser Ser Glu Ala Cys Gly Pro 885 890 895 Glu Thr Ala Glu Ala Lys Val Leu Phe Asp Lys Val Ala Ser Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Gly Pro Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ala 930 935 940 Leu Thr Gly Ala Glu Tyr Lys Pro Val Ser Ala Ala Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Arg Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Ser Asp Gly Gln Lys Lys Asp Ala Ser Lys Gln 980 985 990 Pro Gly Ser Gly Leu Ala Ser Gly Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Arg Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Phe Ile Arg Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Val Ser Thr Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Arg Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Thr Met Val His Gly Asp Asn Val Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Val Leu Ser Glu Glu Asp Arg Asp Ala Leu Ile Ala Lys Cys Ser 1315 1320 1325 Asp Tyr Gln Arg Arg Leu Arg Ser Val Asn Ile Arg Val Lys Val Asp 1330 1335 1340 Ser Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile 1380 1385 1390 Ala Glu Asn Glu Ala Glu Thr Lys Leu Gln Ala Ile Leu Glu Asp Ile 1395 1400 1405 His Val Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Ser Met Glu Asp Phe Gln Lys Val Leu Glu Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Gln Pro Leu Cys Gln 1475 1480 1485 Leu Pro Pro Gly Ala Arg Cys Val Cys Gly Lys Asn Pro Ala Gln Tyr 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 108 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006250488.1 <400> 108 Met Ala Ala Leu Cys Leu Thr Val Asn Ala Gly Asn Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Leu Leu Arg Val Ser Glu Ser Val Val Phe 35 40 45 Thr Asp Thr Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Ala Thr 50 55 60 Ser Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Val Leu Thr 85 90 95 Ser Ala Ile Lys Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Cys Gln Glu His Leu Arg Gln Ser Lys Thr Leu 130 135 140 Val His Val Asn Arg Trp Tyr Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Thr Lys Trp Asp Ala Ser Ala Asn Lys Ala Thr Gly 165 170 175 Ala Pro Asp Arg Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Glu Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Asp Glu Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Arg His Arg Lys Asn Pro Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Arg Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Ile Leu Asp Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly Ala Asp Ala Glu Thr Phe 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Ile His Lys Asn Pro Asp Gly Lys Ile Thr Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr Gln Ala Leu Pro Val Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asp Ser Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ile Ser Lys Lys Glu Thr Gly Ser Ala Pro Lys Glu Arg Pro 725 730 735 Ala Ala Ala Val Ser Ala Pro Cys Ala Ala Ala Glu Asp Ala Ala Ile 740 745 750 Leu Tyr Ser Arg Val Ala Val Gln Gly Asp Ala Val Arg Asp Leu Lys 755 760 765 Ala Lys Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Thr Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Arg 785 790 795 800 Pro Gly Asn Pro Pro Thr Ala Ala Val Gln Thr Val Ser Thr Lys Ser 805 810 815 Pro Ser Asn Thr Gly Glu Tyr Thr Ser Leu Tyr Asn Lys Val Ala Ala 820 825 830 Gln Gly Glu Leu Val Arg Lys Leu Lys Ala Glu Lys Ala Pro Lys Ala 835 840 845 Lys Ile Asn Asp Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Leu Pro Ala Ser 865 870 875 880 Gln Asn Ser His Ser Ser Pro Val Ser Asn Ala Lys Pro Val Gly Ser 885 890 895 Glu Thr Pro Glu Ala Lys Leu Leu Phe Asp Arg Val Ala Cys Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val 915 920 925 Asp Ser Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ser 930 935 940 Leu Thr Gly Ile Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Lys Gln Asn Asp Gly Gln Gly Lys Asp Ser Ser Lys Ser 980 985 990 Gln Gly Ser Gly Leu Ser Ser Gly Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Glu Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ser Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Gly Ala Leu Glu 1075 1080 1085 Lys Glu Lys Asn His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Asp 1170 1175 1180 Glu Val Leu Gln Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Leu Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Ile Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Thr Leu Ser Glu Glu Asp Arg Glu Ala Leu Met Ala Lys Cys Asn 1315 1320 1325 Glu Tyr Arg Arg Arg Leu Leu Gly Ala Asp Ile Arg Ala Arg Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile 1380 1385 1390 Ala Glu Lys Glu Ala Glu Ser Lys Leu Lys Gly Val Leu Glu Asp Ile 1395 1400 1405 Gln Leu Asn Leu Phe Thr Arg Ala Ser Glu Asp Leu Lys Ser His Met 1410 1415 1420 Val Val Ser Asp Thr Leu Glu Asp Phe Gln Lys Val Leu Asp Ser Gly 1425 1430 1435 1440 Lys Val Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Val Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Asn Pro Leu Cys Glu 1475 1480 1485 Leu Gln Pro Gly Ala Met Cys Val Cys Gly Lys Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 109 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006250487.1 <400> 109 Met Ala Ala Leu Cys Leu Thr Val Asn Ala Gly Asn Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Leu Leu Arg Val Ser Glu Ser Val Val Phe 35 40 45 Thr Asp Thr Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Ala Thr 50 55 60 Ser Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Val Leu Thr 85 90 95 Ser Ala Ile Lys Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Cys Gln Glu His Leu Arg Gln Ser Lys Thr Leu 130 135 140 Val His Val Asn Arg Trp Tyr Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Arg Ser Val Gly Thr Lys Trp Asp Ala Ser Ala Asn Lys Ala Thr Gly 165 170 175 Ala Pro Asp Arg Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Glu Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Asp Glu Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ile Glu Ser Arg His Arg Lys Asn Pro Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Arg Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Ile Leu 515 520 525 Asp Ala Gln Glu Glu Met Lys Glu Val Ala Arg His Pro Lys Asn Pro 530 535 540 Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Ile Glu 545 550 555 560 Gly Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Asn Pro Asp Gly 580 585 590 Lys Ile Thr Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr Gln Ala Leu 610 615 620 Pro Val Pro Ala Val Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asp Ser 645 650 655 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Gln Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys 690 695 700 Ile Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Val Glu Ile Ser Lys Lys Glu Thr Gly 725 730 735 Ser Ala Pro Lys Glu Arg Pro Ala Ala Ala Val Ser Ala Pro Cys Ala 740 745 750 Ala Ala Glu Asp Ala Ala Ile Leu Tyr Ser Arg Val Ala Val Gln Gly 755 760 765 Asp Ala Val Arg Asp Leu Lys Ala Lys Lys Ala Pro Lys Glu Asp Ile 770 775 780 Asp Ala Ala Val Lys Gln Leu Leu Thr Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Arg Pro Gly Asn Pro Pro Thr Ala Ala Val 805 810 815 Gln Thr Val Ser Thr Lys Ser Pro Ser Asn Thr Gly Glu Tyr Thr Ser 820 825 830 Leu Tyr Asn Lys Val Ala Ala Gln Gly Glu Leu Val Arg Lys Leu Lys 835 840 845 Ala Glu Lys Ala Pro Lys Ala Lys Ile Asn Asp Ala Val Glu Cys Leu 850 855 860 Leu Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Val 865 870 875 880 Pro Gly Gln Leu Pro Ala Ser Gln Asn Ser His Ser Ser Pro Val Ser 885 890 895 Asn Ala Lys Pro Val Gly Ser Glu Thr Pro Glu Ala Lys Leu Leu Phe 900 905 910 Asp Arg Val Ala Cys Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu 915 920 925 Lys Ala Ser Lys Asp Gln Val Asp Ser Ala Val Gln Glu Leu Leu Gln 930 935 940 Leu Lys Ala Gln Tyr Lys Ser Leu Thr Gly Ile Glu Tyr Lys Pro Val 945 950 955 960 Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu 965 970 975 Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Gln 980 985 990 Gly Lys Asp Ser Ser Lys Ser Gln Gly Ser Gly Leu Ser Ser Gly Gly 995 1000 1005 Ala Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Ala Lys Lys Glu Glu Asn Leu Ala Glu Trp Tyr Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Ser Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp 1060 1065 1070 Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Ile Phe 1075 1080 1085 Val Ser Gln Gly Ala Leu Glu Lys Glu Lys Asn His Ile Ala Asp Phe 1090 1095 1100 Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala 1105 1110 1115 1120 Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala 1125 1130 1135 Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn 1140 1145 1150 Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe 1155 1160 1165 Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala 1170 1175 1180 Thr Phe Glu Glu Ala Ala Asp Glu Val Leu Gln Ile Leu Glu Leu Tyr 1185 1190 1195 1200 Ala Arg Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Arg Gly Arg 1205 1210 1215 Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Leu 1220 1225 1230 Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser 1235 1240 1245 His His Leu Gly Gln Asn Phe Ser Lys Met Cys Glu Ile Val Phe Glu 1250 1255 1260 Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Cys Ser Trp 1265 1270 1275 1280 Gly Leu Thr Thr Arg Thr Ile Gly Val Met Ile Met Val His Gly Asp 1285 1290 1295 Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Ser Val Gln Val Val 1300 1305 1310 Val Ile Pro Cys Gly Ile Thr Asn Thr Leu Ser Glu Glu Asp Arg Glu 1315 1320 1325 Ala Leu Met Ala Lys Cys Asn Glu Tyr Arg Arg Arg Leu Leu Gly Ala 1330 1335 1340 Asp Ile Arg Ala Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp 1345 1350 1355 1360 Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Val Arg Leu Glu Val 1365 1370 1375 Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp 1380 1385 1390 Thr Gly Glu Lys Leu Thr Ile Ala Glu Lys Glu Ala Glu Ser Lys Leu 1395 1400 1405 Lys Gly Val Leu Glu Asp Ile Gln Leu Asn Leu Phe Thr Arg Ala Ser 1410 1415 1420 Glu Asp Leu Lys Ser His Met Val Val Ser Asp Thr Leu Glu Asp Phe 1425 1430 1435 1440 Gln Lys Val Leu Asp Ser Gly Lys Val Ala Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln 1460 1465 1470 Asp Val Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Asn Pro Leu Cys Glu Leu Gln Pro Gly Ala Met Cys Val Cys 1490 1495 1500 Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 110 <211> 1488 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006094389.1 <400> 110 Met Ala Thr Leu Cys Leu Thr Val Asn Ser Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Phe Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Val Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Met Thr 50 55 60 Ala Arg Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Pro 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Val Ala Trp Gln Glu Gln Leu Gln Gln Asn Arg Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys Ala Lys Gly 165 170 175 Ala Pro Gln Lys Lys Pro Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Phe Lys Phe Met Ala Glu Thr His Thr Asp Tyr Val Leu Gly Asn Gly 210 215 220 Lys Val Ile Leu Glu Asp Val Ala Met Leu His Ile Glu Pro Asp Gln 225 230 235 240 Phe Thr Tyr Thr Ser Asp His Phe Glu Thr Ile Met Lys Tyr Ala Glu 245 250 255 Lys Leu Ile Gln Glu Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala Glu 260 265 270 Arg Met Lys Ala Glu Arg Glu Gln Arg Thr Glu Ser Lys His Arg Asn 275 280 285 Asn Ser Val Glu Lys Asn Leu Gln Met Trp Glu Glu Met Lys Lys Gly 290 295 300 Ser Pro Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile Asp Met Asn 305 310 315 320 Ser Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys Ile 325 330 335 Gln Pro His Pro Arg Thr Gly Asn Arg Tyr Asn Val Tyr Pro Thr Tyr 340 345 350 Asp Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val Thr His Ala 355 360 365 Leu Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp Ile 370 375 380 Ile Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser Arg 385 390 395 400 Leu Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu Thr Trp Phe 405 410 415 Val Asp Glu Gly Leu Val Asp Gly Trp Asp Asp Pro Arg Phe Pro Thr 420 425 430 Val Arg Gly Val Leu Arg Arg Gly Met Thr Val Glu Gly Leu Lys Gln 435 440 445 Phe Ile Ala Ala Gln Gly Ser Ser Arg Ser Val Val Asn Met Glu Trp 450 455 460 Asp Lys Ile Trp Ala Phe Asn Lys Lys Val Ile Asp Pro Val Ala Pro 465 470 475 480 Arg Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val His Val Pro 485 490 495 Gly Ala Gln Glu Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Pro 500 505 510 Asp Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Arg Val Phe Ile Glu 515 520 525 Gly Ala Asp Ala Glu Thr Leu Ser Glu Gly Glu Met Val Thr Phe Ile 530 535 540 Asn Trp Gly Asn Leu Asn Ile Thr Lys Ile His Lys Asn Ala Asn Gly 545 550 555 560 Lys Ile Val Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr 565 570 575 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Ala His Ala Leu 580 585 590 Pro Ile Pro Ala Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 595 600 605 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 610 615 620 Lys His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 625 630 635 640 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 645 650 655 Gln Pro Tyr Glu Pro Val Ser Pro His Ser Cys Arg Glu Ala Pro Cys 660 665 670 Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 675 680 685 Gly Ser Lys Glu Lys Thr Lys Val Glu Thr Leu Lys Asn Glu Thr Thr 690 695 700 Thr Pro Val Lys Glu Arg Pro Ala Ala Pro Leu Thr Asn Thr Ser Ala 705 710 715 720 Ala Ser Glu Gly Pro Val Val Leu Tyr Asn Lys Val Ala Ala Gln Gly 725 730 735 Asp Val Val Arg Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp Val 740 745 750 Asp Ala Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu 755 760 765 Lys Thr Gly Gln Glu Tyr Lys Pro Gly Ser Pro Pro Ala Ala Ala Ala 770 775 780 Gln Ala Val Ser Ser Pro Ser Ser Ala Gly Pro Pro Gly Gly Arg Ser 785 790 795 800 Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys 805 810 815 Ala Glu Lys Ala Pro Lys Ala Lys Val Asn Glu Ala Val Glu Cys Leu 820 825 830 Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Xaa Glu Tyr Val 835 840 845 Pro Gly Pro Ala Pro Val Thr His Gly Ser Gly Ser Ser Pro Ala Arg 850 855 860 Xaa Ser Glu Pro Cys Gly Pro Glu Thr Xaa Glu Ala Lys Val Leu Phe 865 870 875 880 Asp Gln Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu 885 890 895 Lys Ala Ser Lys Asp Gln Val Asp Ala Ala Val Gln Glu Leu Leu Gln 900 905 910 Arg Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val Glu Tyr Lys Pro Val 915 920 925 Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu 930 935 940 Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Ser 945 950 955 960 Lys Lys Glu Pro Ser Arg Gly Gln Gly Gly Gly Gly Leu Pro Pro Ser 965 970 975 Gly Ala Gly Glu Gly Gln Gly Pro Arg Lys Gln Thr Arg Leu Gly Leu 980 985 990 Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln Val Ile 995 1000 1005 Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile 1010 1015 1020 Leu Arg Pro Trp Ala Phe Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe 1025 1030 1035 1040 Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met 1045 1050 1055 Phe Val Ser Gln Ser Ala Leu Glu Lys Glu Lys Thr His Val Ala Asp 1060 1065 1070 Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu 1075 1080 1085 Ala Glu Pro Ile Ala Val Arg Pro Thr Ser Glu Thr Val Met Tyr Pro 1090 1095 1100 Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu 1105 1110 1115 1120 Asn Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro 1125 1130 1135 Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe 1140 1145 1150 Ala Thr Phe Glu Glu Ala Ala Ala Glu Val Leu Gln Ile Leu Asp Leu 1155 1160 1165 Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly 1170 1175 1180 Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr 1185 1190 1195 1200 Val Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr 1205 1210 1215 Ser His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Ile Phe 1220 1225 1230 Glu Asp Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser 1235 1240 1245 Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly 1250 1255 1260 Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val 1265 1270 1275 1280 Val Val Ile Pro Cys Gly Ile Thr Asn Ala Leu Ser Glu Glu Asp Arg 1285 1290 1295 Asp Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser 1300 1305 1310 Val Asn Ile Arg Ala Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly 1315 1320 1325 Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu 1330 1335 1340 Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg 1345 1350 1355 1360 Asp Thr Gly Glu Lys Leu Thr Val Thr Glu Asn Glu Ala Glu Thr Lys 1365 1370 1375 Leu Lys Ala Leu Leu Glu Asp Ile His Ala Asn Leu Phe Arg Lys Ala 1380 1385 1390 Ser Glu Asp Leu Lys Thr His Met Val Val Ala Asn Ser Met Glu Asp 1395 1400 1405 Phe Gln Lys Leu Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys 1410 1415 1420 Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp 1425 1430 1435 1440 Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys 1445 1450 1455 Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Arg Cys Val 1460 1465 1470 Cys Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1475 1480 1485 <210> 111 <211> 1513 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005409334.1 <400> 111 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Ala Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Ala Ala Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Val Leu Arg Val Ser Asp Asn Val Val Phe 35 40 45 Thr Asp Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ala Asn Leu Met Glu His Ala Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Ile Asn Asp Leu Asn His Cys Leu Ser Leu Arg Thr Phe Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Phe Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Asn Ala Ala Trp Leu Glu Glu Ser Lys Gln Ser Lys Gly Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Arg Val Phe 145 150 155 160 Gln Ser Val Ser Ala Lys Trp Gly Val Val Ala Ser Gln Ala Arg Gly 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Lys Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Val Glu Ser Arg His Arg Asn Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Arg 355 360 365 Ser Arg Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asp Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Ile Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Gly Ser Arg Val Leu Ile Glu Gly Ala Asp Ala Gln Thr Leu 545 550 555 560 Ala Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Asn Ile His Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Gly 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala Arg Ala Leu Pro Ile Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys Arg Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Gly Glu Ala Thr Lys Thr Glu Val Ile Pro Ala Pro Lys Glu Arg Ala 725 730 735 Ala Pro Pro Val Asn Lys Ser Cys Ala Thr Ser Glu Ala Ser Leu Val 740 745 750 Leu His Asn Arg Val Ala Ala Gln Gly Asp Val Val Arg Glu Leu Lys 755 760 765 Ala Arg Lys Ala Ala Lys Asp Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Asn Pro Pro Ala Ala Gly Ala Leu Gly Ala Ser Ser Gln Val 805 810 815 Pro Ala Ala Ala Ser Ile Gln Glu Ser Arg Ser Leu Tyr Asp Arg Val 820 825 830 Ala Ala Gln Gly Glu Leu Val Arg Lys Leu Lys Ala Glu Lys Ala Leu 835 840 845 Lys Ala Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala 850 855 860 Glu Tyr Lys Glu Lys Thr Gly Lys Asp Tyr Val Pro Gly Gln Pro Pro 865 870 875 880 Ser Ala Thr Leu Glu Pro His Pro Ala Ser Asn Tyr Ala Pro Ala Gly 885 890 895 Pro Glu Thr Pro Glu Ala Arg Ala Leu Tyr Asp Arg Val Ala Ala Gln 900 905 910 Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Thr Asp Gln 915 920 925 Val Asp Pro Ala Val Gln Ala Leu Leu Glu Leu Lys Ala Gln Tyr Lys 930 935 940 Ala Leu Val Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu 945 950 955 960 Asp Arg Gly Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln 965 970 975 Asn Lys Pro Gln Thr Pro Asp Asp Gly His Gly Lys Asp Pro Ala Lys 980 985 990 Ser Gln Gly Ser Gly Leu Ala Pro Ser Gly Ala Gly Glu Gly Gln Gly 995 1000 1005 Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Arg Lys Glu Glu Asn 1010 1015 1020 Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu 1025 1030 1035 1040 Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser 1045 1050 1055 Ile Trp Glu Ser Ile Gln Ala Phe Phe Asn Ala Glu Ile Lys Lys Leu 1060 1065 1070 Gly Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ser Ala Leu 1075 1080 1085 Glu Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp 1090 1095 1100 Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg 1105 1110 1115 1120 Pro Thr Ser Glu Thr Val Met Tyr Pro Thr Tyr Ala Lys Trp Val Gln 1125 1130 1135 Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val 1140 1145 1150 Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe 1155 1160 1165 Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala 1170 1175 1180 Glu Glu Val Leu Gln Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu 1185 1190 1195 1200 Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys 1205 1210 1215 Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala 1220 1225 1230 Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn 1235 1240 1245 Phe Ser Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Lys Pro Gly 1250 1255 1260 Glu Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr 1265 1270 1275 1280 Ile Gly Val Met Ile Met Val His Gly Asp Asn Met Gly Leu Val Leu 1285 1290 1295 Pro Pro Arg Val Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile 1300 1305 1310 Thr Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Met Ala Lys Cys 1315 1320 1325 Asn Asp Tyr Arg Thr Arg Leu Leu Ser Ala Asn Ile Arg Ala Arg Val 1330 1335 1340 Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu 1345 1350 1355 1360 Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys 1365 1370 1375 Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr 1380 1385 1390 Val Ala Glu Ser Glu Ala Glu Ala Lys Leu Glu Ala Met Leu Glu Glu 1395 1400 1405 Ile His Ala Asn Leu Phe Gln Arg Ala Ser Glu Asp Leu Lys Thr His 1410 1415 1420 Met Val Val Ala Asp Thr Met Glu Asp Leu Gln Lys Thr Leu Asp Ser 1425 1430 1435 1440 Gly Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Glu Cys Glu Asp 1445 1450 1455 Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala 1460 1465 1470 Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Gln Pro Leu Arg 1475 1480 1485 Glu Leu Pro Pro Gly Ala Val Cys Val Cys Gly Arg Ser Pro Ala Lys 1490 1495 1500 Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 112 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004626936.1 <400> 112 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Ala Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Ala Ala Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Val Leu Arg Val Ser Glu Asn Val Ala Phe 35 40 45 Thr Glu Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Ser Thr 50 55 60 Ala Gly Leu Tyr Gly Ala Asn Leu Met Glu His Ala Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Phe Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Phe Cys Val Trp Ala Thr Leu 115 120 125 Lys Glu Asn Ala Ala Trp Gln Glu Glu Leu Lys Gln Ser Lys Gly Pro 130 135 140 Val His Val Lys Arg Trp Phe Ser Phe Leu Glu Ala Gln Arg Val Phe 145 150 155 160 Gln Ser Val Ser Ala Lys Trp Gly Val Ser Ala Ser Gln Ala Arg Thr 165 170 175 Ala Pro Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Lys Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Val Glu Ser Arg His Arg Asn Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Arg 355 360 365 Ser Arg Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Gly Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Asp Val Ile Pro Val Asn Val Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Lys Val Leu Ile Glu Gly Ala Asp Ala Gln Thr Leu 545 550 555 560 Ala Glu Gly Glu Thr Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Ser Ile His Arg Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Gly 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Val Thr 595 600 605 Trp Leu Ala Glu Thr Ala Arg Ala Leu Pro Val Pro Thr Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Arg Asp Ser Lys Arg Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Gly Lys Thr Lys 705 710 715 720 Gly Glu Thr Thr Lys Pro Glu Val Ser Pro Pro Leu Lys Glu Arg Ala 725 730 735 Ala Pro Pro Val Asn Lys Ser Cys Thr Met Ser Glu Asp Ser Leu Ala 740 745 750 Leu His Arg Arg Val Ala Val Gln Gly Asp Met Val Arg Glu Leu Lys 755 760 765 Ala Arg Lys Ala Pro Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Val Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Ala Ser Pro Pro Ala Ala Gly Ala Gln Asp Val Ser Ser Gln Ala 805 810 815 Pro Thr Ala Leu Gln Glu Gly Arg Thr Leu Tyr Asp Arg Val Ala Ala 820 825 830 Gln Gly Glu Leu Val Arg Arg Leu Lys Ala Glu Lys Ala Leu Gln Ala 835 840 845 Lys Ile Asn Glu Ala Val Ala Cys Leu Leu Ser Leu Lys Ala Ala Tyr 850 855 860 Lys Glu Gln Thr Gly Lys Asp Tyr Val Pro Gly Gln Pro Pro Pro Ala 865 870 875 880 Pro Asn Pro Glu Ser Leu Pro Ala Arg Asp His Glu Pro Ala Gly Pro 885 890 895 Asp Thr Ala Glu Ala Arg Ala Leu Tyr Asp Arg Val Ala Ala Gln Gly 900 905 910 Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Thr Asp Gln Val 915 920 925 Asp Pro Ala Val Gln Val Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ala 930 935 940 Leu Ala Gly Val Glu Tyr Lys Pro Val Ser Thr Thr Gly Ala Glu Asp 945 950 955 960 Arg Gly Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Ser Gln Thr Pro Ser Asp Ser Gln Gly Lys Glu Pro Ser Lys Ser 980 985 990 Gln Gly Ser Gly Val Ala Pro Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ala Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Gln Ala Phe Phe Asn Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ser Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Thr Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Leu Gln Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Arg Gly Lys Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Cys Glu Ile Ile Phe Glu Asp Pro Lys Thr Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Ile Met Val His Gly Asp Asn Met Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Ser Val Gln Val Val Ile Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Met Ala Lys Cys Ser 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Gly Ala Asn Ile Arg Ala Arg Val Asp 1330 1335 1340 Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Cys Val Ala Val Arg Arg Asp Thr Gly Ala Lys Leu Thr Val 1380 1385 1390 Ala Glu Ser Glu Ala Glu Thr Lys Leu Gly Ala Leu Leu Glu Glu Ile 1395 1400 1405 His Val Asn Leu Phe Gln Arg Ala Ser Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Ala Asn Thr Met Glu Asp Phe Gln Lys Thr Leu Asp Ser Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Glu Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Gln Pro Leu Gln Val 1475 1480 1485 Leu Pro Pro Gly Ala Gln Cys Val Cys Gly Arg Asn Pro Ala Arg Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 113 <211> 1513 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004613070.1 <400> 113 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Asp Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser Ile Ser Ile 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ala Phe 35 40 45 Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Ser 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Gln Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Val Thr Lys Leu Ser Ser Cys Asp Leu Phe Pro 85 90 95 Ser Ala Leu Asn Glu Leu Asn Leu Gly Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Thr Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Ser Ala Ala Trp Gln Glu Glu Leu Arg Gln Ser Lys Ala Pro 130 135 140 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Gln Pro Phe 145 150 155 160 Arg Ser Val Gly Ala Lys Trp Asn Ile Ser Pro Val Arg Ala Lys Val 165 170 175 Ala Ser Glu Lys Lys Gln Asp Val Gly Lys Phe Ile Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Ala Glu Ser Lys His Arg Gly Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Pro Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Thr Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asp Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Phe Val Ala Leu Leu Lys 500 505 510 Arg Glu Val Val Pro Val Asn Ile Pro Glu Ala Arg Glu Glu Met Lys 515 520 525 Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Arg Val Phe Ile Glu Gly Ala Asp Ala Asp Thr Leu 545 550 555 560 Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Asn Ile 565 570 575 Thr Lys Val His Lys Ser Ala Asp Gly Lys Ile Val Ser Leu Asp Ala 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Asp Ser Thr Arg Val Pro Pro Val Pro Ala Val Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Ile Asn Arg Asn Thr Lys His Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Arg Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ala Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Val Glu Ala Ser Lys Thr Glu Thr Ala Ala Ala Leu Lys Gly Arg Ala 725 730 735 Ala Pro Ala Val Ser Asp Pro Arg Ala Ala Val Glu Asp Pro Met Val 740 745 750 Leu Tyr Asn Arg Val Ala Thr Gln Gly Asp Ala Val Arg Glu Leu Lys 755 760 765 Ala Arg Lys Ala Thr Lys Glu Glu Ile Asp Ala Ala Val Arg Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Cys Pro Pro Ala Ala Gly Pro Pro Lys Thr Thr Ala Gln Ala 805 810 815 Ala Pro Ser Ala Leu Ser Ala Gln Ala Leu Tyr Glu Ala Val Ala Ala 820 825 830 Gln Gly Glu Ala Val Arg Lys Leu Lys Ala Glu Lys Ala Ala Lys Ala 835 840 845 Lys Ile Gly Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Gln Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Asp Tyr Val Pro Pro Ala Gln Pro Leu Pro 865 870 875 880 Pro Gln Ser Ser Gly Ala Ala Pro Ala Gln Thr Ser Gln Pro Ser Ala 885 890 895 Pro Glu Thr Pro Glu Ala Lys Ala Leu Phe Asn Lys Val Ala Ala Gln 900 905 910 Gly Asp Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln 915 920 925 Val Asp Gly Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln Tyr Lys 930 935 940 Ala Leu Thr Gly Val Glu Tyr Lys Pro Val Thr Ala Thr Gly Pro Glu 945 950 955 960 Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln 965 970 975 Asn Lys Pro Gln Lys Gln Asn Asp Gly Pro Lys Lys Glu Pro Ser Lys 980 985 990 Ser Pro Gly Ser Gly Leu Ser Ser Gly Gly Ala Gly Glu Gly Gln Gly 995 1000 1005 Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn 1010 1015 1020 Leu Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ala Glu Met Ile Glu 1025 1030 1035 1040 Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Phe Ser 1045 1050 1055 Ile Trp Glu Ala Ile Lys Asp Phe Phe Asp Ser Glu Ile Lys Lys Leu 1060 1065 1070 Gly Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln Ser Ala Leu 1075 1080 1085 Glu Thr Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp 1090 1095 1100 Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Val Arg 1105 1110 1115 1120 Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln 1125 1130 1135 Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val 1140 1145 1150 Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe 1155 1160 1165 Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ser 1170 1175 1180 Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Gln Val Tyr Glu Asp 1185 1190 1195 1200 Leu Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys 1205 1210 1215 Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala 1220 1225 1230 Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn 1235 1240 1245 Phe Ser Lys Met Phe Glu Ile Ile Phe Glu Asp Pro Lys Met Pro Gly 1250 1255 1260 Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp Gly Leu Thr Thr Arg Thr 1265 1270 1275 1280 Ile Gly Val Met Thr Met Val His Gly Asp Asn Met Gly Leu Val Leu 1285 1290 1295 Pro Pro Arg Val Ala Cys Val Gln Val Val Val Ile Pro Cys Gly Ile 1300 1305 1310 Thr Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Ile Ala Lys Cys 1315 1320 1325 Asn Asp Tyr Arg Arg Arg Leu Leu Gly Ala Asn Ile Arg Val Arg Val 1330 1335 1340 Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu 1345 1350 1355 1360 Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys 1365 1370 1375 Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr 1380 1385 1390 Val Ala Glu Ala Glu Ala Glu Thr Arg Leu Arg Ala Leu Leu Glu Asp 1395 1400 1405 Ile His Ala Ser Leu Phe Ala Arg Ala Ser Glu Asp Leu Arg Thr His 1410 1415 1420 Met Val Val Ser Asp Thr Met Glu Asp Phe Gln Lys Ala Leu Asp Ser 1425 1430 1435 1440 Gly Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp 1445 1450 1455 Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala 1460 1465 1470 Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Gln Pro Leu Arg 1475 1480 1485 Gln Leu Gln Pro Gly Ala Arg Cys Ile Cys Gly Lys Asn Pro Ala Lys 1490 1495 1500 Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 114 <211> 1575 <212> PRT <213> Artificial Sequence <220> <223> EPRS KFO25396.1 <400> 114 Met Glu Leu Val Ser Gly Val Gly Glu Glu Lys Ala Gly Ala Pro Ser 1 5 10 15 Ser Gly Lys Pro Val Ala His Pro Glu Ser Thr Thr Glu Asp Ala Thr 20 25 30 Glu His Arg Leu Ser Phe Gln Phe Ser Ser Arg Leu Pro Ser Arg Pro 35 40 45 His Arg Pro Glu Lys Ile Pro Ser Ser Phe Cys Pro Thr Phe Pro Tyr 50 55 60 Ala Phe Ala Asp Phe Arg Gly Arg Met Leu Ala Phe Arg Ile Leu Glu 65 70 75 80 Ala Leu Leu Ala Val Glu His Val Lys Gly Asp Val Ser Ile Ser Val 85 90 95 Glu Glu Gly Lys Glu Asn Val Leu Arg Val Ser Glu Asn Val Ala Phe 100 105 110 Thr Glu Val Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Ala Thr 115 120 125 Ala Gly Leu Tyr Gly Thr Asn Pro Met Glu His Ala Glu Ile Asp His 130 135 140 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 145 150 155 160 Ser Ala Val Asp Glu Leu Asn His Cys Leu Ser Leu Arg Thr Phe Leu 165 170 175 Val Gly Asn Ser Leu Thr Leu Ala Asp Phe Cys Val Trp Ala Thr Leu 180 185 190 Lys Gly Asn Ala Ala Trp Gln Glu Ala Ser Thr His Ser Arg Gly Pro 195 200 205 Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln Arg Val Phe 210 215 220 Gln Ser Val Gly Ala Lys Trp Gly Val Ala Ala Ser Lys Ala Arg Gly 225 230 235 240 Ala Ser Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 245 250 255 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 260 265 270 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 275 280 285 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 290 295 300 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 305 310 315 320 Ala Met Leu Arg Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 325 330 335 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Lys Glu Gly Lys 340 345 350 Ala Phe Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 355 360 365 Gln Arg Val Glu Ser Arg His Arg Asn Asn Ser Val Glu Lys Asn Leu 370 375 380 Gln Met Trp Glu Glu Met Lys Gln Gly Ser Pro Phe Gly Gln Ser Cys 385 390 395 400 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 405 410 415 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 420 425 430 Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 435 440 445 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 450 455 460 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Gly Ala Leu Gly Ile Arg 465 470 475 480 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 485 490 495 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val His Glu Gly Leu Val Asp 500 505 510 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 515 520 525 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 530 535 540 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 545 550 555 560 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 565 570 575 Lys Glu Val Val Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys 580 585 590 Glu Ala Ala Arg His Pro Lys Asn Pro Glu Val Gly Leu Lys Ser Val 595 600 605 Trp Tyr Gly Pro Arg Val Leu Ile Glu Gly Ala Asp Ala Glu Thr Leu 610 615 620 Ala Glu Gly Glu Thr Val Thr Phe Ile Asn Trp Gly Asn Val Ala Ile 625 630 635 640 Ser Arg Ile His Arg Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Gly 645 650 655 Lys Leu Asn Leu Glu Asn Lys Asp His Lys Lys Thr Thr Lys Ile Thr 660 665 670 Trp Leu Ala Glu Thr Ala Arg Ala Leu Pro Val Pro Thr Ile Cys Val 675 680 685 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 690 695 700 Phe Lys Gln Tyr Ile Asn Arg Asn Ser Lys Arg Glu Glu Leu Met Leu 705 710 715 720 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 725 730 735 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 740 745 750 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 755 760 765 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 770 775 780 Val Glu Ala Thr Lys Ala Glu Thr Ser Pro Ala Leu Lys Glu Arg Pro 785 790 795 800 Ala Pro Ser Val Asn Lys Ser Trp Ala Thr Thr Glu Asp Ser Leu Val 805 810 815 Leu Tyr Asn Arg Val Ala Ala Gln Gly Asp Thr Val Arg Glu Leu Lys 820 825 830 Ala Arg Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 835 840 845 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 850 855 860 Pro Gly Ser Pro Pro Ala Ala Gly Ala Gln Asp Val Ser Ser Gln Ser 865 870 875 880 Gln Ala Thr Ala Gln Glu Ser Arg Ser Leu Tyr Asp Arg Val Ala Thr 885 890 895 Gln Gly Glu Leu Val Arg Lys Leu Lys Ala Glu Lys Ala Leu Lys Ala 900 905 910 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr 915 920 925 Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ala Pro 930 935 940 Ser Ser Glu Ser Ser Pro Ala Gly Asp Arg Glu Pro Val Gly Pro Glu 945 950 955 960 Ala Pro Glu Ala Arg Ala Leu Phe Ala Arg Ala Ala Ala Gln Gly Glu 965 970 975 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Glu 980 985 990 Pro Ala Val Gln Ala Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ala Leu 995 1000 1005 Ala Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp Arg 1010 1015 1020 Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Ser Lys 1025 1030 1035 1040 Pro Gln Lys Pro Ser Asp Gly His Gly Lys Asp Ser Ser Lys Ser Pro 1045 1050 1055 Gly Ser Gly Leu Ala Pro Gly Gly Ser Gly Glu Gly Gln Gly Pro Arg 1060 1065 1070 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 1075 1080 1085 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr 1090 1095 1100 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1105 1110 1115 1120 Glu Ser Ile Lys Ala Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1125 1130 1135 Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Thr Ala Leu Glu Lys 1140 1145 1150 Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1155 1160 1165 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1170 1175 1180 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1185 1190 1195 1200 Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1205 1210 1215 Glu Phe Lys His Pro Gln Pro Leu Leu Arg Thr Arg Glu Phe Leu Trp 1220 1225 1230 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1235 1240 1245 Val Leu Gln Ile Leu Glu Leu Tyr Thr Arg Val Tyr Glu Asp Leu Leu 1250 1255 1260 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1265 1270 1275 1280 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1285 1290 1295 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1300 1305 1310 Lys Met Cys Glu Ile Val Phe Glu Asp Pro Arg Thr Pro Gly Glu Lys 1315 1320 1325 Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1330 1335 1340 Val Met Val Met Val His Gly Asp Asn Thr Gly Leu Val Leu Pro Pro 1345 1350 1355 1360 Arg Val Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Val Thr Asn 1365 1370 1375 Ala Leu Cys Glu Glu Asp Arg Ala Ala Leu Met Ala Lys Cys Ser Glu 1380 1385 1390 Tyr Arg Arg Arg Leu Leu Gly Ala Asp Ile Arg Ala Arg Val Asp Leu 1395 1400 1405 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1410 1415 1420 Gly Val Pro Val Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1425 1430 1435 1440 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val Ala 1445 1450 1455 Glu Ser Glu Ala Glu Ala Arg Leu Gln Ala Leu Leu Glu Asp Ile His 1460 1465 1470 Ala Ser Leu Phe Arg Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1475 1480 1485 Val Ala Asp Thr Met Glu Asp Phe Gln Lys Thr Leu Asp Ser Gly Lys 1490 1495 1500 Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1505 1510 1515 1520 Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1525 1530 1535 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Arg Pro Leu Arg Glu Leu 1540 1545 1550 Gln Pro Gly Ala Arg Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr 1555 1560 1565 Thr Leu Phe Gly Arg Ser Tyr 1570 1575 <210> 115 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_003474570.1 <400> 115 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Ala Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Thr Ala Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ala Phe 35 40 45 Ala Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ala Asn Leu Met Glu His Ala Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Ile Asp Glu Leu Asn His Cys Leu Ser Leu Arg Thr Phe Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Phe Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Ser Ala Ala Trp Gln Glu Glu Ser Lys Gln Ser Lys Gly Pro 130 135 140 Val His Val Arg Arg Trp Phe Ser Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Ser Ala Lys Trp Gly Ile Ala Ala Asn Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Lys Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Val Glu Ser Arg His Arg Asn Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Ser Arg Tyr Asn Ile Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Ala Val Pro Val Asn Ile Pro Glu Ala Gln Glu Glu Met Lys 515 520 525 Glu Val Ala Arg His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Ser Arg Val Leu Ile Glu Gly Ala Asp Ala Gln Thr Leu 545 550 555 560 Ala Glu Gly Glu Thr Val Thr Phe Ile Asn Trp Gly Asn Ile Ser Ile 565 570 575 Thr Ser Ile His Lys Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Gly 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Thr Arg Ala Leu Pro Thr Pro Thr Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys Arg Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Glu Pro Tyr Glu Pro Thr Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Gly Glu Ala Thr Lys Thr Glu Val Lys Gly Thr Cys Leu His Met Ser 725 730 735 Leu Pro Pro Val Asn Lys Ser Gly Ala Ser Ser Glu Asp Pro Leu Val 740 745 750 Leu His Lys Arg Val Ala Ala Gln Gly Asp Met Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly His Pro Pro Ala Ala Gly Ala Gln Asp Val Ser Cys Arg Ser 805 810 815 Leu Ala Thr Val Gln Glu Ser Arg Ser Leu Tyr Asp Lys Val Ala Val 820 825 830 Gln Gly Glu Leu Val Arg Lys Leu Lys Ala Glu Lys Ala Leu Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr 850 855 860 Lys Ala Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ser Ala 865 870 875 880 Val Ser Ser Glu Ala Arg Pro Ala Gly Ser Cys Glu Pro Ala Gly Pro 885 890 895 Glu Ser Leu Glu Ala Arg Ala Leu Tyr Asp Arg Val Ala Ala Gln Gly 900 905 910 Glu Ala Val Arg Lys Leu Lys Ala Glu Glu Ala Pro Ala Asp Gln Val 915 920 925 Asp Ala Ala Val Gln Val Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ala 930 935 940 Leu Ala Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Arg Gly Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Thr Pro Asn Gly Ser His Gly Lys Asp Ser Ser Lys Ser 980 985 990 Gln Gly Ser Gly Leu Ala Pro Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Ala Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ser Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Thr Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Met Gln Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Cys Glu Ile Ile Phe Glu Asp Pro Lys Ser Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Val Met Val His Gly Asp Asn Thr Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Ala Ile Gln Val Val Ile Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Leu Ala Lys Cys Glu 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Gly Ala Asp Val Arg Ala Arg Val Asp 1330 1335 1340 Leu Arg Glu Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Ser Glu Ala Glu Pro Arg Leu Gln Ala Met Leu Glu Glu Ile 1395 1400 1405 Gln Ala His Leu Phe Gln Arg Ala Leu Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Val Asn Thr Met Glu Asp Phe Gln Lys Thr Leu Asp Ala Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Glu Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Gln Pro Leu Gln Glu 1475 1480 1485 Leu Pro Pro Gly Ala Arg Cys Val Cys Ser Arg Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 116 <211> 1512 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_005005650.1 <400> 116 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Ala Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Thr Ala Glu His Val Lys Gly Asp Val Ser Ile Ser Val 20 25 30 Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn Val Ala Phe 35 40 45 Ala Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Thr Thr 50 55 60 Ala Gly Leu Tyr Gly Ala Asn Leu Met Glu His Ala Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Leu Phe Thr 85 90 95 Ser Ala Ile Asp Glu Leu Asn His Cys Leu Ser Leu Arg Thr Phe Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Phe Cys Val Trp Ala Thr Leu 115 120 125 Lys Gly Ser Ala Ala Trp Gln Glu Glu Ser Lys Gln Ser Lys Gly Pro 130 135 140 Val His Val Arg Arg Trp Phe Ser Phe Leu Glu Ala Gln Gln Ala Phe 145 150 155 160 Gln Ser Val Ser Ala Lys Trp Gly Ile Ala Ala Asn Lys Ala Arg Val 165 170 175 Ala Pro Glu Lys Lys Gln Asp Ile Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Lys Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Val Glu Ser Arg His Arg Asn Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Ser Arg Tyr Asn Ile Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Leu Arg Ala Leu Cys Lys Lys Val Ile Asp Pro Val Ala Pro 500 505 510 Arg Tyr Val Ala Leu Leu Lys Lys Glu Ala Val Pro Val Asn Ile Pro 515 520 525 Glu Ala Gln Glu Glu Met Lys Glu Val Ala Arg His Pro Lys Asn Pro 530 535 540 Glu Val Gly Leu Lys Pro Val Trp Tyr Ser Ser Arg Val Leu Ile Glu 545 550 555 560 Gly Ala Asp Ala Gln Thr Leu Ala Glu Gly Glu Thr Val Thr Phe Ile 565 570 575 Asn Trp Gly Asn Ile Ser Ile Thr Ser Ile His Lys Asn Ala Asp Gly 580 585 590 Lys Ile Val Ser Leu Asp Gly Lys Leu Asn Leu Glu Asn Lys Asp Tyr 595 600 605 Lys Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr Arg Ala Leu 610 615 620 Pro Thr Pro Thr Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro 625 630 635 640 Val Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser 645 650 655 Lys Arg Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys 660 665 670 Lys Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp 675 680 685 Glu Pro Tyr Glu Pro Thr Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys 690 695 700 Val Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser 705 710 715 720 Gly Ser Lys Glu Lys Thr Lys Gly Glu Ala Thr Lys Thr Glu Met Ser 725 730 735 Leu Pro Pro Val Asn Lys Ser Gly Ala Ser Ser Glu Asp Pro Leu Val 740 745 750 Leu His Lys Arg Val Ala Ala Gln Gly Asp Met Val Arg Glu Leu Lys 755 760 765 Ala Lys Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly His Pro Pro Ala Ala Gly Ala Gln Asp Val Ser Cys Arg Ser 805 810 815 Leu Ala Thr Val Gln Glu Ser Arg Ser Leu Tyr Asp Lys Val Ala Val 820 825 830 Gln Gly Glu Leu Val Arg Lys Leu Lys Ala Glu Lys Ala Leu Lys Ala 835 840 845 Lys Ile Asn Glu Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr 850 855 860 Lys Ala Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ser Ala 865 870 875 880 Val Ser Ser Glu Ala Arg Pro Ala Gly Ser Cys Glu Pro Ala Gly Pro 885 890 895 Glu Ser Leu Glu Ala Arg Ala Leu Tyr Asp Arg Val Ala Ala Gln Gly 900 905 910 Glu Ala Val Arg Lys Leu Lys Ala Glu Glu Ala Pro Ala Asp Gln Val 915 920 925 Asp Ala Ala Val Gln Val Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ala 930 935 940 Leu Ala Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Glu Asp 945 950 955 960 Arg Gly Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu Lys Gln Asn 965 970 975 Lys Pro Gln Thr Pro Asn Gly Ser His Gly Lys Asp Ser Ser Lys Ser 980 985 990 Gln Gly Ser Gly Leu Ala Pro Ser Gly Ala Gly Glu Gly Gln Gly Pro 995 1000 1005 Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu 1010 1015 1020 Ala Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr 1025 1030 1035 1040 Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile 1045 1050 1055 Trp Glu Ser Ile Lys Ala Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly 1060 1065 1070 Val Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ser Ala Leu Glu 1075 1080 1085 Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu Val Ala Trp Val 1090 1095 1100 Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro 1105 1110 1115 1120 Thr Ser Glu Thr Val Met Tyr Pro Thr Tyr Ala Lys Trp Val Gln Ser 1125 1130 1135 His Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg 1140 1145 1150 Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu 1155 1160 1165 Trp Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu 1170 1175 1180 Glu Val Met Gln Ile Leu Glu Leu Tyr Ala Arg Val Tyr Glu Glu Leu 1185 1190 1195 1200 Leu Ala Ile Pro Val Val Arg Gly Arg Lys Thr Glu Lys Glu Lys Phe 1205 1210 1215 Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser 1220 1225 1230 Gly Arg Ala Ile Gln Gly Gly Thr Ser His His Leu Gly Gln Asn Phe 1235 1240 1245 Ser Lys Met Cys Glu Ile Ile Phe Glu Asp Pro Lys Ser Pro Gly Glu 1250 1255 1260 Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile 1265 1270 1275 1280 Gly Val Met Val Met Val His Gly Asp Asn Thr Gly Leu Val Leu Pro 1285 1290 1295 Pro Arg Val Ala Ala Ile Gln Val Val Ile Ile Pro Cys Gly Ile Thr 1300 1305 1310 Asn Ala Leu Ser Glu Glu Asp Lys Glu Ala Leu Leu Ala Lys Cys Glu 1315 1320 1325 Asp Tyr Arg Arg Arg Leu Leu Gly Ala Asp Val Arg Ala Arg Val Asp 1330 1335 1340 Leu Arg Glu Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu 1345 1350 1355 1360 Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser 1365 1370 1375 Cys Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Val 1380 1385 1390 Ala Glu Ser Glu Ala Glu Pro Arg Leu Gln Ala Met Leu Glu Glu Ile 1395 1400 1405 Gln Ala His Leu Phe Gln Arg Ala Leu Glu Asp Leu Lys Thr His Met 1410 1415 1420 Val Val Val Asn Thr Met Glu Asp Phe Gln Lys Thr Leu Asp Ala Gly 1425 1430 1435 1440 Lys Ile Ala Gln Ile Pro Phe Cys Gly Glu Ile Glu Cys Glu Asp Trp 1445 1450 1455 Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro 1460 1465 1470 Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Gln Pro Leu Gln Glu 1475 1480 1485 Leu Pro Pro Gly Ala Arg Cys Val Cys Ser Arg Asn Pro Ala Lys Phe 1490 1495 1500 Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 117 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004867452.1 <400> 117 Met Ala Thr Leu Cys Leu Thr Val Asn Ala Gly Ala Pro Pro Leu Glu 1 5 10 15 Ala Leu Leu Ala Ala Glu His Val Lys Glu Asp Val Ser Ile Ser Val 20 25 30 Glu Gly Gly Lys Glu Asn Val Leu Arg Val Ser Glu Asn Val Ala Phe 35 40 45 Thr Asp Val Tyr Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Ala Thr 50 55 60 Ala Gly Leu Tyr Gly Ala Asn Val Met Glu His Ala Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ala Ser Cys Ala Ser Phe Ala 85 90 95 Ser Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Phe Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Phe Cys Val Trp Ala Ala Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Glu Ala Thr Gln Ser Lys Gly Pro 130 135 140 Val His Val Lys Arg Trp Phe Ser Phe Leu Glu Ala Gln Arg Val Phe 145 150 155 160 Gln Ser Val Ser Ala Lys Trp Gly Val Pro Ala Ser Lys Thr Gln Ala 165 170 175 Ala Ser Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Lys Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Val Glu Ser Arg His Arg Ser Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Ser Arg Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Val Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val His Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Leu Val Pro Glu Val Gln Glu Glu Met Lys 515 520 525 Glu Ala Ala Arg His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Arg Val Leu Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Thr Glu Gly Glu Thr Val Thr Phe Ile Asn Trp Gly Asn Ile Ser Ile 565 570 575 Thr Lys Ile His Arg Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Gly 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala Gln Ala Leu Pro Val Pro Thr Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Ile Asn Lys Asn Ser Lys Arg Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Gly Glu Ala Thr Lys Thr Glu Met Ser Pro Ser Leu Lys Glu Arg Pro 725 730 735 Ala Pro Pro Val Tyr Lys Ser Trp Ala Pro Ser Glu Asp Phe Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Thr Gln Gly Asp Thr Val Arg Glu Leu Lys 755 760 765 Ala Arg Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Met Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Ser Pro Pro Ala Ala Gly Ala Gln Glu Ala Ala Ser Gln Ser 805 810 815 Pro Ala Ala Ala Leu Glu Ser Arg Ser Leu Tyr Asp Arg Val Ala Ala 820 825 830 Gln Gly Glu Leu Val Arg Lys Leu Lys Ala Glu Lys Ala Leu Lys Ala 835 840 845 Lys Ile Asn Gln Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ala Pro 865 870 875 880 Ser Ser Lys Ser Ser Pro Ala Gly Asp Arg Glu Ser Ser Gly Pro Glu 885 890 895 Ala Pro Glu Ala Gln Val Leu Phe Ala Arg Val Ala Ala Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp 915 920 925 Pro Ala Val Gln Val Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ala Leu 930 935 940 Ala Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Asp Asp Arg 945 950 955 960 Asp Lys Lys Lys Lys Asp Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Ala His Asp Gly His Gly Lys Glu Pro Phe Asn Ser Gln 980 985 990 Gly Ser Gly Pro Thr Pro Gly Gly Ala Gly Glu Gly Gln Gly Pro Arg 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Ala Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ser Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Glu Leu Tyr Thr Arg Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1250 1255 1260 Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Val Met Val His Gly Asp Asn Thr Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Gly Asp Arg Glu Ala Leu Met Val Lys Cys Ser Asp 1315 1320 1325 Tyr Cys Arg Arg Leu Leu Gly Ala Asp Ile Arg Thr Arg Val Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala 1380 1385 1390 Glu Ser Glu Ala Glu Ala Lys Leu Glu Ala Leu Leu Glu Asp Ile His 1395 1400 1405 Ala Ser Leu Phe Arg Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asp Thr Met Glu Asp Phe Gln Lys Thr Leu Asp Ala Gly Lys 1425 1430 1435 1440 Val Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Thr Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Arg Pro Leu Gln Glu Leu 1475 1480 1485 Gln Pro Gly Ala Arg Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 118 <211> 1511 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_004878984.1 <400> 118 Met Ala Thr Leu Cys Leu Thr Val Thr Gly Gly Ala Pro Pro Arg Glu 1 5 10 15 Ala Leu Leu Ala Ala Glu His Val Lys Glu Asp Val Ser Ile Ser Val 20 25 30 Glu Gly Gly Lys Glu Asn Val Leu Arg Val Ser Glu Asn Val Ala Phe 35 40 45 Thr Asp Val Tyr Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala Ala Thr 50 55 60 Ala Gly Leu Tyr Gly Ala Asn Val Met Glu His Ala Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ala Ser Cys Ala Ser Phe Ala 85 90 95 Ser Ala Val Asn Glu Leu Asn His Cys Leu Ser Leu Arg Thr Phe Leu 100 105 110 Val Gly Asn Ser Leu Thr Leu Ala Asp Phe Cys Val Trp Ala Ala Leu 115 120 125 Lys Gly Asn Ala Ala Trp Gln Glu Glu Ala Thr Gln Ser Lys Gly Pro 130 135 140 Val His Val Lys Arg Trp Phe Ser Phe Leu Glu Ala Gln Arg Val Phe 145 150 155 160 Gln Ser Val Ser Ala Lys Trp Gly Val Pro Ala Ser Lys Thr Gln Ala 165 170 175 Ala Ser Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly 180 185 190 Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala Ser Gly 195 200 205 Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr 210 215 220 Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn 225 230 235 240 Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val 245 250 255 Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His 260 265 270 Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Lys Glu Gly Lys 275 280 285 Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu Arg Glu 290 295 300 Gln Arg Val Glu Ser Arg His Arg Ser Asn Ser Val Glu Lys Asn Leu 305 310 315 320 Gln Met Trp Glu Glu Met Lys Lys Gly Ser Gln Phe Gly Gln Ser Cys 325 330 335 Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys Met Arg 340 345 350 Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro Arg Thr Gly 355 360 365 Ser Arg Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val 370 375 380 Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His 385 390 395 400 Asp Arg Asp Glu Gln Phe Tyr Trp Val Ile Glu Ala Leu Gly Ile Arg 405 410 415 Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val 420 425 430 Leu Ser Lys Arg Lys Leu Thr Trp Phe Val His Glu Gly Leu Val Asp 435 440 445 Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg 450 455 460 Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser 465 470 475 480 Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn 485 490 495 Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala Leu Leu Lys 500 505 510 Lys Glu Val Val Pro Val Leu Val Pro Glu Val Gln Glu Glu Met Lys 515 520 525 Glu Ala Ala Arg His Pro Lys Asn Pro Glu Val Gly Leu Lys Pro Val 530 535 540 Trp Tyr Ser Pro Arg Val Leu Ile Glu Gly Ala Asp Ala Glu Thr Leu 545 550 555 560 Thr Glu Gly Glu Thr Val Thr Phe Ile Asn Trp Gly Asn Ile Ser Ile 565 570 575 Thr Lys Ile His Arg Asn Ala Asp Gly Lys Ile Val Ser Leu Asp Gly 580 585 590 Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr 595 600 605 Trp Leu Ala Glu Thr Ala Gln Ala Leu Pro Val Pro Thr Ile Cys Val 610 615 620 Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp 625 630 635 640 Phe Lys Gln Tyr Ile Asn Lys Asn Ser Lys Arg Glu Glu Leu Met Leu 645 650 655 Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile Ile Gln Leu 660 665 670 Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser 675 680 685 Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp 690 695 700 Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys 705 710 715 720 Gly Glu Ala Thr Lys Thr Glu Met Ser Pro Ser Leu Lys Glu Arg Pro 725 730 735 Ala Pro Pro Val Tyr Lys Ser Trp Ala Pro Ser Glu Asp Phe Leu Val 740 745 750 Leu Tyr Asn Arg Val Ala Thr Gln Gly Asp Thr Val Arg Glu Leu Lys 755 760 765 Ala Arg Lys Ala Ala Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Lys Glu Met Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Ser Pro Pro Ala Ala Gly Ala Gln Glu Ala Ala Ser Gln Ser 805 810 815 Pro Ala Ala Ala Leu Glu Ser Arg Ser Leu Tyr Asp Arg Val Ala Ala 820 825 830 Gln Gly Glu Leu Val Arg Lys Leu Lys Ala Glu Lys Ala Leu Lys Ala 835 840 845 Lys Ile Asn Gln Ala Val Glu Cys Leu Leu Ser Leu Lys Ala Glu Tyr 850 855 860 Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro Gly Gln Pro Pro Ala Pro 865 870 875 880 Ser Ser Lys Ser Ser Pro Ala Gly Asp Arg Glu Ser Ser Gly Pro Glu 885 890 895 Ala Pro Glu Ala Gln Val Leu Phe Ala Arg Val Ala Ala Gln Gly Glu 900 905 910 Val Val Arg Lys Leu Lys Ala Glu Lys Ala Ser Lys Asp Gln Val Asp 915 920 925 Pro Ala Val Gln Val Leu Leu Gln Leu Lys Ala Gln Tyr Lys Ala Leu 930 935 940 Ala Gly Val Glu Tyr Lys Pro Val Ser Ala Thr Gly Ala Asp Asp Arg 945 950 955 960 Asp Lys Lys Lys Lys Asp Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys 965 970 975 Pro Gln Lys Ala His Asp Gly His Gly Lys Glu Pro Phe Asn Ser Gln 980 985 990 Gly Ser Gly Pro Thr Pro Gly Gly Ala Gly Glu Gly Gln Gly Pro Arg 995 1000 1005 Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala 1010 1015 1020 Asp Trp Tyr Ser Gln Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr 1025 1030 1035 1040 Asp Val Ser Gly Cys Tyr Ile Leu Arg Pro Trp Ala Tyr Ser Ile Trp 1045 1050 1055 Glu Ser Ile Lys Ala Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val 1060 1065 1070 Glu Asn Cys Tyr Phe Pro Ile Phe Val Ser Gln Ser Ala Leu Glu Lys 1075 1080 1085 Glu Lys Thr His Val Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr 1090 1095 1100 Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile Ala Ile Arg Pro Thr 1105 1110 1115 1120 Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His 1125 1130 1135 Arg Asp Leu Pro Ile Arg Leu Asn Gln Trp Cys Asn Val Val Arg Trp 1140 1145 1150 Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr Arg Glu Phe Leu Trp 1155 1160 1165 Gln Glu Gly His Ser Ala Phe Ala Thr Phe Glu Glu Ala Ala Glu Glu 1170 1175 1180 Val Leu Gln Ile Leu Glu Leu Tyr Thr Arg Val Tyr Glu Glu Leu Leu 1185 1190 1195 1200 Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu Lys Glu Lys Phe Ala 1205 1210 1215 Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Phe Ile Ser Ala Ser Gly 1220 1225 1230 Arg Ala Ile Gln Gly Ala Thr Ser His His Leu Gly Gln Asn Phe Ser 1235 1240 1245 Lys Met Cys Glu Ile Val Phe Glu Asp Pro Lys Thr Pro Gly Glu Lys 1250 1255 1260 Gln Phe Ala Tyr Gln Cys Ser Trp Gly Leu Thr Thr Arg Thr Ile Gly 1265 1270 1275 1280 Val Met Val Met Val His Gly Asp Asn Thr Gly Leu Val Leu Pro Pro 1285 1290 1295 Arg Val Ala Ser Val Gln Val Val Val Ile Pro Cys Gly Ile Thr Asn 1300 1305 1310 Ala Leu Ser Glu Gly Asp Arg Glu Ala Leu Met Val Lys Cys Ser Asp 1315 1320 1325 Tyr Cys Arg Arg Leu Leu Gly Ala Asp Ile Arg Thr Arg Val Asp Leu 1330 1335 1340 Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn His Trp Glu Leu Lys 1345 1350 1355 1360 Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg Asp Met Lys Ser Cys 1365 1370 1375 Gln Phe Val Ala Val Arg Arg Asp Thr Gly Glu Lys Leu Thr Ile Ala 1380 1385 1390 Glu Ser Glu Ala Glu Ala Lys Leu Glu Ala Leu Leu Glu Asp Ile His 1395 1400 1405 Ala Ser Leu Phe Arg Arg Ala Ser Glu Asp Leu Lys Thr His Met Val 1410 1415 1420 Val Ala Asp Thr Met Glu Asp Phe Gln Lys Thr Leu Asp Ala Gly Lys 1425 1430 1435 1440 Val Ala Gln Ile Pro Phe Cys Gly Glu Ile Asp Cys Glu Asp Trp Ile 1445 1450 1455 Lys Lys Thr Thr Thr Arg Asp Gln Asp Leu Glu Pro Gly Ala Pro Ser 1460 1465 1470 Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Arg Pro Leu Gln Glu Leu 1475 1480 1485 Gln Pro Gly Ala Arg Cys Val Cys Gly Lys Asn Pro Ala Lys Phe Tyr 1490 1495 1500 Thr Leu Phe Gly Arg Ser Tyr 1505 1510 <210> 119 <211> 1616 <212> PRT <213> Artificial Sequence <220> <223> EPRS EPQ16188.1 <400> 119 Met Ser Ala Glu Thr His Val Arg Val Thr Leu Gln Gln Val Phe His 1 5 10 15 Arg Thr Cys Ala Leu Leu Ala Val Glu His Val Lys Asp Asn Val Ser 20 25 30 Ile Phe Val Glu Glu Gly Lys Glu Asn Ile Leu Arg Val Ser Glu Asn 35 40 45 Val Val Phe Thr Asp Ile Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val 50 55 60 Ala Met Thr Ala Lys Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu 65 70 75 80 Ile Asp His Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asn 85 90 95 Leu Phe Pro Ser Ala Ile Asn Glu Leu Asn His Cys Leu Ser Leu Arg 100 105 110 Thr Tyr Leu Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp 115 120 125 Ala Thr Leu Lys Gly Asn Val Ala Trp Gln Glu Gln Leu Gln Gln Asn 130 135 140 Arg Ala Pro Val His Val Lys Arg Trp Phe Gly Phe Leu Glu Ala Gln 145 150 155 160 Gln Ala Phe Gln Ser Val Gly Thr Lys Trp Asn Val Ser Ala Thr Lys 165 170 175 Ala Lys Gly Ala Pro Gln Lys Lys Pro Asp Val Gly Lys Phe Val Glu 180 185 190 Leu Pro Gly Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu 195 200 205 Ala Ser Gly Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn 210 215 220 Gln His Tyr Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp 225 230 235 240 Asp Thr Asn Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu 245 250 255 Glu Asp Val Ala Met Leu His Ile Glu Pro Asp Gln Phe Thr Tyr Thr 260 265 270 Ser Asp His Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln 275 280 285 Glu Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala Glu Arg Met Lys Ala 290 295 300 Glu Arg Glu Gln Arg Thr Glu Ser Lys His Arg Asn Asn Ser Val Glu 305 310 315 320 Lys Asn Leu Gln Met Trp Glu Glu Met Lys Lys Gly Ser Pro Phe Gly 325 330 335 Gln Ser Cys Cys Leu Arg Ala Lys Ile Asp Met Asn Ser Asn Asn Gly 340 345 350 Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln Pro His Pro 355 360 365 Arg Thr Gly Asn Arg Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys 370 375 380 Pro Ile Val Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr 385 390 395 400 Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu 405 410 415 Gly Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn 420 425 430 Asn Thr Val Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asp Glu Gly 435 440 445 Leu Val Asp Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val 450 455 460 Leu Arg Arg Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala 465 470 475 480 Gln Gly Ser Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp 485 490 495 Ala Phe Asn Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Val Ala 500 505 510 Leu Leu Lys Lys Glu Val Ile Pro Val His Val Pro Gly Ala Gln Glu 515 520 525 Glu Met Lys Glu Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu 530 535 540 Lys Pro Val Trp Tyr Ser Pro Arg Val Phe Ile Glu Gly Ala Asp Ala 545 550 555 560 Glu Thr Leu Ser Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn 565 570 575 Ile Asn Ile Thr Lys Ile His Lys Asn Ala Asn Gly Lys Ile Val Ser 580 585 590 Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Thr 595 600 605 Lys Ile Thr Trp Leu Ala Glu Thr Ala His Ala Leu Pro Ile Pro Ala 610 615 620 Ile Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys 625 630 635 640 Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asn Ser Lys His Glu Glu 645 650 655 Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys Gly Asp Ile 660 665 670 Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu 675 680 685 Pro Val Ser Pro His Ser Cys Arg Glu Ala Pro Cys Val Leu Ile Phe 690 695 700 Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu 705 710 715 720 Lys Thr Lys Val Glu Thr Val Lys Asn Glu Thr Thr Thr Pro Val Lys 725 730 735 Glu Arg Pro Val Ala Pro Leu Thr Asn Thr Ser Ala Ala Ser Glu Gly 740 745 750 Pro Met Val Leu Tyr Asn Lys Val Ala Ala Gln Gly Asp Val Val Arg 755 760 765 Glu Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp Val Asp Ala Ala Val 770 775 780 Lys His Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln 785 790 795 800 Glu Tyr Lys Pro Gly Ser Pro Pro Ala Ala Ala Ala Ala Gln Ala Val 805 810 815 Ser Ser Pro Ser Ser Ala Ser Pro Pro Gly Gly Arg Ser Leu Tyr Asp 820 825 830 Glu Val Ala Ala Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys 835 840 845 Ala Pro Lys Ala Val Ser Ser Pro Ala Ser Ala Ser Pro Pro Gly Gly 850 855 860 Arg Ser Leu Tyr Asp Glu Val Ala Ala Gln Gly Glu Val Val Arg Lys 865 870 875 880 Leu Lys Ala Glu Lys Ala Pro Lys Ala Lys Val Asn Glu Ala Val Glu 885 890 895 Cys Leu Leu Ser Leu Lys Ala Gln Tyr Lys Glu Lys Thr Gly Gln Glu 900 905 910 Tyr Val Pro Gly Gln Pro Pro Leu Pro Leu Gly Ser Gly Ser Ser Pro 915 920 925 Ala Arg Ser Ser Glu Pro Cys Gly Pro Glu Thr Ala Glu Ala Lys Val 930 935 940 Leu Phe Asp Gln Val Ala Ser Gln Gly Glu Val Val Arg Lys Leu Lys 945 950 955 960 Ala Glu Lys Ala Ser Lys Asp Gln Val Asp Ala Ala Val Gln Glu Leu 965 970 975 Leu Gln Arg Lys Ala Gln Tyr Lys Ser Leu Thr Gly Val Glu Tyr Lys 980 985 990 Pro Val Ser Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu 995 1000 1005 Lys Glu Asn Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp 1010 1015 1020 Gly Ser Lys Lys Glu Pro Ser Arg Gly Gln Gly Gly Gly Gly Leu Pro 1025 1030 1035 1040 Pro Ser Gly Ala Gly Glu Gly Gln Gly Pro Arg Lys Gln Thr Arg Leu 1045 1050 1055 Gly Leu Glu Ala Lys Lys Glu Glu Asn Leu Ala Asp Trp Tyr Ser Gln 1060 1065 1070 Val Ile Thr Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys 1075 1080 1085 Tyr Ile Leu Arg Pro Trp Ala Phe Ser Ile Trp Glu Ser Ile Lys Asp 1090 1095 1100 Phe Phe Asp Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe 1105 1110 1115 1120 Pro Met Phe Val Ser Gln Ser Ala Leu Glu Lys Glu Lys Thr His Val 1125 1130 1135 Ala Asp Phe Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr 1140 1145 1150 Glu Leu Ala Glu Pro Ile Ala Val Arg Pro Thr Ser Glu Thr Val Met 1155 1160 1165 Tyr Pro Ala Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile 1170 1175 1180 Arg Leu Asn Gln Trp Cys Asn Val Val Val Val Met Tyr Pro Ala Tyr 1185 1190 1195 1200 Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln 1205 1210 1215 Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu 1220 1225 1230 Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr 1235 1240 1245 Phe Glu Glu Ala Ala Ala Glu Arg Trp Glu Phe Lys His Pro Gln Pro 1250 1255 1260 Phe Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe 1265 1270 1275 1280 Ala Thr Phe Glu Glu Ala Ala Ala Glu Val Leu Gln Ile Leu Asp Leu 1285 1290 1295 Tyr Ala Gln Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Lys Gly 1300 1305 1310 Arg Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr 1315 1320 1325 Val Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr 1330 1335 1340 Ser His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Ile Phe 1345 1350 1355 1360 Glu Asp Pro Lys Met Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser 1365 1370 1375 Trp Gly Leu Thr Thr Arg Thr Ile Gly Val Met Thr Met Val His Gly 1380 1385 1390 Asp Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val 1395 1400 1405 Val Val Ile Pro Cys Gly Ile Thr Thr Ala Leu Ser Glu Glu Asp Arg 1410 1415 1420 Asp Ala Leu Ile Ala Lys Cys Asn Asp Tyr Arg Arg Arg Leu Leu Ser 1425 1430 1435 1440 Val Asn Ile Arg Ala Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly 1445 1450 1455 Trp Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu 1460 1465 1470 Val Gly Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg 1475 1480 1485 Asp Thr Gly Glu Lys Leu Thr Val Thr Glu Asn Glu Ala Glu Thr Lys 1490 1495 1500 Leu Gln Ala Leu Leu Glu Asp Ile His Ala Asn Leu Phe Arg Lys Ala 1505 1510 1515 1520 Ser Glu Asp Leu Lys Thr His Met Val Val Ala Asn Ser Met Glu Asp 1525 1530 1535 Phe Gln Lys Leu Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys 1540 1545 1550 Gly Glu Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp 1555 1560 1565 Gln Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys 1570 1575 1580 Ile Pro Phe Lys Pro Leu Cys Glu Leu Gln Pro Gly Ala Arg Cys Val 1585 1590 1595 1600 Cys Gly Lys Asn Pro Ala Lys Tyr Tyr Thr Leu Phe Gly Arg Ser Tyr 1605 1610 1615 <210> 120 <211> 1486 <212> PRT <213> Artificial Sequence <220> <223> EPRS NP_001019409.1 <400> 120 Met Ala Ala Leu Cys Leu Thr Val Asn Ala Gly Asn Pro Pro Leu Gly 1 5 10 15 Cys Arg Val Lys Val Asn Lys Arg Asn Lys Ser Phe Met Leu Ser Phe 20 25 30 Leu Glu Ala Leu Leu Ala Val Glu His Val Lys Gly Asp Val Ser Ile 35 40 45 Ser Val Glu Glu Gly Lys Glu Asn Leu Leu Arg Val Ser Glu Ser Val 50 55 60 Val Phe Thr Asp Thr Asn Ser Ile Leu Arg Tyr Leu Ala Arg Val Ala 65 70 75 80 Ala Thr Ser Gly Leu Tyr Gly Ser Asn Leu Met Glu His Thr Glu Ile 85 90 95 Asp His Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Ser Cys Asp Val 100 105 110 Leu Thr Ser Ala Ile Lys Glu Leu Asn His Cys Leu Ser Leu Arg Thr 115 120 125 Tyr Leu Val Gly Asn Ser Leu Thr Leu Ala Asp Leu Cys Val Trp Ala 130 135 140 Thr Leu Lys Gly Asn Ala Ala Cys Gln Glu His Leu Arg Gln Ser Lys 145 150 155 160 Thr Leu Val His Val Asn Arg Trp Tyr Gly Phe Leu Glu Ala Gln Gln 165 170 175 Ala Phe Arg Ser Val Gly Thr Lys Trp Asp Ala Ser Ala Asn Lys Ala 180 185 190 Thr Gly Ala Pro Asp Arg Lys Gln Asp Ile Gly Lys Phe Val Glu Leu 195 200 205 Pro Gly Ala Glu Met Gly Lys Val Thr Val Arg Phe Pro Pro Glu Ala 210 215 220 Ser Gly Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln 225 230 235 240 His Tyr Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp 245 250 255 Thr Asn Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu 260 265 270 Asp Val Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser 275 280 285 Asp His Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Glu Glu 290 295 300 Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala Asp Glu Met Lys Ala Glu 305 310 315 320 Pro Val Glu Lys Asn Leu Gln Met Trp Glu Glu Met Lys Arg Gly Ser 325 330 335 Gln Phe Gly Gln Ser Cys Cys Leu Arg Ala Lys Ile Asp Met Ser Ser 340 345 350 Asn Asn Gly Cys Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys Ile Gln 355 360 365 Pro His Pro Arg Thr Gly Asn Lys Tyr Asn Val Tyr Pro Thr Tyr Asp 370 375 380 Phe Ala Cys Pro Ile Val Asp Ser Ile Glu Gly Val Thr His Ala Leu 385 390 395 400 Arg Thr Thr Glu Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile 405 410 415 Glu Ala Leu Gly Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu 420 425 430 Asn Leu Asn Asn Thr Val Leu Ser Lys Arg Lys Leu Thr Trp Phe Val 435 440 445 Asn Glu Gly Leu Gly Ser Ser Arg Ser Val Val Asn Met Glu Trp Asp 450 455 460 Lys Ile Trp Ala Phe Asn Lys Lys Val Ile Asp Pro Val Ala Pro Arg 465 470 475 480 Tyr Val Ala Leu Leu Lys Lys Glu Val Ile Pro Val Asn Ile Leu Asp 485 490 495 Ala Gln Glu Glu Met Lys Glu Val Ala Arg His Pro Lys Asn Pro Asp 500 505 510 Val Gly Leu Lys Pro Val Trp Tyr Ser Pro Lys Val Phe Ile Glu Gly 515 520 525 Ala Asp Ala Glu Thr Phe Ser Glu Gly Glu Met Val Thr Phe Ile Asn 530 535 540 Trp Gly Asn Ile Asn Ile Thr Lys Ile His Lys Asn Pro Asp Gly Lys 545 550 555 560 Ile Thr Ser Leu Asp Ala Lys Leu Asn Leu Glu Asn Lys Asp Tyr Lys 565 570 575 Lys Thr Thr Lys Ile Thr Trp Leu Ala Glu Thr Thr Gln Ala Leu Pro 580 585 590 Val Pro Ala Val Cys Val Thr Tyr Glu His Leu Ile Thr Lys Pro Val 595 600 605 Leu Gly Lys Asp Glu Asp Phe Lys Gln Tyr Val Asn Lys Asp Ser Lys 610 615 620 His Glu Glu Leu Met Leu Gly Asp Pro Cys Leu Lys Asp Leu Lys Lys 625 630 635 640 Gly Asp Ile Ile Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln 645 650 655 Pro Tyr Glu Pro Val Ser Pro Tyr Ser Cys Lys Glu Ala Pro Cys Ile 660 665 670 Leu Ile Tyr Ile Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser Gly 675 680 685 Ser Lys Glu Lys Thr Lys Val Glu Ile Ser Lys Lys Glu Thr Gly Ser 690 695 700 Ala Pro Lys Glu Arg Pro Ala Ala Ala Val Ser Ala Pro Cys Ala Ala 705 710 715 720 Ala Glu Asp Ala Ala Ile Leu Tyr Ser Arg Val Ala Val Gln Gly Asp 725 730 735 Ala Val Arg Asp Leu Lys Ala Lys Lys Ala Pro Lys Glu Asp Ile Asp 740 745 750 Ala Ala Val Lys Gln Leu Leu Thr Leu Lys Ala Glu Tyr Lys Glu Lys 755 760 765 Thr Gly Gln Glu Tyr Arg Pro Gly Asn Pro Pro Thr Ala Ala Val Gln 770 775 780 Thr Val Ser Thr Lys Ser Pro Ser Asn Thr Gly Glu Tyr Thr Ser Leu 785 790 795 800 Tyr Asn Lys Val Ala Ala Gln Gly Glu Leu Val Arg Lys Leu Lys Ala 805 810 815 Glu Lys Ala Pro Lys Ala Lys Ile Asn Asp Ala Val Glu Cys Leu Leu 820 825 830 Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Lys Glu Tyr Val Pro 835 840 845 Gly Gln Leu Pro Ala Ser Gln Asn Ser His Ser Ser Pro Val Ser Asn 850 855 860 Ala Lys Pro Val Gly Ser Glu Thr Pro Glu Ala Lys Leu Leu Phe Asp 865 870 875 880 Arg Val Ala Cys Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys 885 890 895 Ala Ser Lys Asp Gln Val Asp Ser Ala Val Gln Glu Leu Leu Gln Leu 900 905 910 Lys Ala Gln Tyr Lys Ser Leu Thr Gly Ile Glu Tyr Lys Pro Val Ser 915 920 925 Ala Thr Gly Ala Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn 930 935 940 Lys Ser Glu Lys Gln Asn Lys Pro Gln Lys Gln Asn Asp Gly Gln Gly 945 950 955 960 Lys Asp Ser Ser Lys Ser Gln Gly Ser Gly Leu Ser Ser Gly Gly Ala 965 970 975 Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala 980 985 990 Lys Lys Glu Glu Asn Leu Ala Glu Trp Tyr Ser Gln Val Ile Thr Lys 995 1000 1005 Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu Arg 1010 1015 1020 Pro Trp Ser Tyr Ser Ile Trp Glu Ser Ile Lys Asp Phe Phe Asp Ala 1025 1030 1035 1040 Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Ile Phe Val 1045 1050 1055 Ser Gln Gly Ala Leu Glu Lys Glu Lys Asn His Ile Ala Asp Phe Ala 1060 1065 1070 Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu 1075 1080 1085 Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr 1090 1095 1100 Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Arg Leu Asn Gln 1105 1110 1115 1120 Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu 1125 1130 1135 Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Ser Ala Phe Ala Thr 1140 1145 1150 Phe Glu Glu Ala Ala Asp Glu Val Leu Gln Ile Leu Glu Leu Tyr Ala 1155 1160 1165 Arg Val Tyr Glu Glu Leu Leu Ala Ile Pro Val Val Arg Gly Arg Lys 1170 1175 1180 Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Leu Glu 1185 1190 1195 1200 Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His 1205 1210 1215 His Leu Gly Gln Asn Phe Ser Lys Met Cys Glu Ile Val Phe Glu Asp 1220 1225 1230 Pro Lys Thr Pro Gly Glu Lys Gln Phe Ala Tyr Gln Cys Ser Trp Gly 1235 1240 1245 Leu Thr Thr Arg Thr Ile Gly Val Met Ile Met Val His Gly Asp Asn 1250 1255 1260 Met Gly Leu Val Leu Pro Pro Arg Val Ala Ser Val Gln Val Val Val 1265 1270 1275 1280 Ile Pro Cys Gly Ile Thr Asn Thr Leu Ser Glu Glu Asp Arg Glu Ala 1285 1290 1295 Leu Met Ala Lys Cys Asn Glu Tyr Arg Arg Arg Leu Leu Gly Ala Asp 1300 1305 1310 Ile Arg Ala Arg Val Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys 1315 1320 1325 Phe Asn His Trp Glu Leu Lys Gly Val Pro Val Arg Leu Glu Val Gly 1330 1335 1340 Pro Arg Asp Met Lys Ser Cys Gln Phe Val Ala Val Arg Arg Asp Thr 1345 1350 1355 1360 Gly Glu Lys Leu Thr Ile Ala Glu Lys Glu Ala Glu Ser Lys Leu Lys 1365 1370 1375 Gly Val Leu Glu Asp Ile Gln Leu Asn Leu Phe Thr Arg Ala Ser Glu 1380 1385 1390 Asp Leu Lys Ser His Met Val Val Ser Asp Thr Leu Glu Asp Phe Gln 1395 1400 1405 Lys Val Leu Asp Ser Gly Lys Val Ala Gln Ile Pro Phe Cys Gly Glu 1410 1415 1420 Ile Asp Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp 1425 1430 1435 1440 Val Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro 1445 1450 1455 Phe Asn Pro Leu Cys Glu Leu Gln Pro Gly Ala Met Cys Val Cys Gly 1460 1465 1470 Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1475 1480 1485 <210> 121 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_008177290.1 <400> 121 Met Ala Leu Ser Leu Thr Val Asn Ser Glu Val Pro Pro Leu Gly Ala 1 5 10 15 Leu Leu Thr Val Glu His Val Lys Asn Asp Val Lys Ile Ser Val Glu 20 25 30 Glu Gly Lys Glu Thr Ile Leu Arg Val Ser Asp His Ala Val Phe Thr 35 40 45 Asp Val Asn Ser Ile Val Arg Tyr Leu Ala Arg Val Ala Thr Ser Ala 50 55 60 Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His Trp 65 70 75 80 Leu Glu Phe Ser Ala Thr Lys Leu Ser Thr Gly Ala Gln Phe Gly Ser 85 90 95 Ala Leu Leu Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val 100 105 110 Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Val Leu Lys 115 120 125 Gly Ser Ser Ala Trp Gln Glu Gln Leu Gln Gln Asn Lys Ala Pro Val 130 135 140 His Met Lys Arg Trp Tyr Gly Phe Leu Glu Ala Gln Cys Ala Phe Gln 145 150 155 160 Ser Val Gly Thr Lys Trp Ala Ala Thr Ala Ser Lys Thr Lys Val Ala 165 170 175 Thr Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala 180 185 190 Glu Met Gly Lys Val Ile Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr 195 200 205 Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln 210 215 220 Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro 225 230 235 240 Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala 245 250 255 Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe 260 265 270 Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala 275 280 285 Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Met Glu Arg Glu Gln 290 295 300 Arg Ile Glu Ser Lys His Arg Asn Asn Ser Val Glu Lys Asn Leu Gln 305 310 315 320 Met Trp Glu Glu Met Lys Lys Gly Thr Glu Cys Gly Gln Met Cys Cys 325 330 335 Leu Arg Ala Lys Ile Asp Met Asn Ser Asn Asn Gly Cys Met Arg Asp 340 345 350 Pro Thr Leu Tyr Arg Cys Lys Asn Gln Pro His Pro Arg Thr Ala Ser 355 360 365 Asn Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp 370 375 380 Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp 385 390 395 400 Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys 405 410 415 Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu 420 425 430 Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly 435 440 445 Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly 450 455 460 Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser 465 470 475 480 Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ser Phe Asn Lys 485 490 495 Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Thr Ala Leu Leu Lys Asp 500 505 510 Glu Val Val Thr Val Asn Val Pro Glu Ala Leu Asp Glu Met Lys Glu 515 520 525 Val Ala Lys His Pro Lys Asn Ala Asp Val Gly Leu Lys Pro Val Trp 530 535 540 Tyr Ser Ser Lys Val Leu Ile Asp Gly Ala Asp Ala Glu Thr Leu Thr 545 550 555 560 Glu Gly Glu Thr Val Thr Phe Ile Asn Trp Gly Asn Ile Ile Ile Thr 565 570 575 Lys Ile His Arg Asn Ser Ser Arg Lys Ile Val Ser Ile Asp Ala Lys 580 585 590 Leu Asn Met Glu Asn Lys Asp Tyr Lys Lys Thr Thr Lys Ile Thr Trp 595 600 605 Leu Ala Glu Thr Pro His Ala Pro Leu Ile Pro Ala Val Cys Val Asn 610 615 620 Tyr Asp His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe 625 630 635 640 Lys Gln Tyr Val Asn Arg Asn Ser Lys Gln Glu Glu Leu Met Leu Gly 645 650 655 Asp Pro Cys Leu Lys Glu Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln 660 665 670 Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Val Ser Pro 675 680 685 Tyr Ser Cys Lys Glu Ala Pro Cys Val Leu Ile Tyr Ile Pro Asp Gly 690 695 700 His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Ala 705 710 715 720 Glu Thr Ala Lys Lys Glu Ala Ser Ser Thr Ser Lys Gly Lys Ser Val 725 730 735 Pro Leu Val Ala Asp Thr Cys Ile Pro Ala Ser Ala Pro Ser Glu Asp 740 745 750 Pro Leu Val Phe Tyr Asn Arg Val Ser Ala Gln Gly Asp Val Val Arg 755 760 765 Asp Leu Lys Gly Lys Lys Ala Ala Lys Glu Asp Ile Asp Thr Ala Val 770 775 780 Lys Gln Leu Leu Ala Leu Lys Val Glu Tyr Lys Glu Lys Thr Gly Gln 785 790 795 800 Glu Tyr Lys Pro Gly Asn Pro Pro Ala Ala Ala Val Ala Val Pro Asn 805 810 815 Val Ser Ser Thr Pro Gln Thr Ser Ser Asn Leu Asp Ser Lys Ala Leu 820 825 830 Tyr His Lys Val Ala Glu Gln Gly Glu Val Val Arg Lys Leu Lys Ala 835 840 845 Glu Lys Ala Ser Lys Asp Lys Val Asp Glu Ala Val Lys Leu Leu Leu 850 855 860 Ser Leu Lys Ala Glu Tyr Lys Glu Lys Thr Gly Gln Asp Tyr Lys Pro 865 870 875 880 Gly His Val Pro Ala Thr Gln Ile Ser Thr Xaa Pro Gln Thr Thr Ser 885 890 895 Thr Glu Ile Asn Gly Pro Asp Thr Pro Glu Ala Lys Ala Leu Phe Asn 900 905 910 Lys Val Ala Cys Gln Gly Glu Glu Val Arg Lys Leu Lys Ala Gly Lys 915 920 925 Ala Glu Lys Asp Lys Ile Asp Thr Ala Val Gln Glu Leu Leu Gln Leu 930 935 940 Lys Ala Gln Tyr Lys Ser Leu Ala Gly Val Asp Tyr Lys Pro Val Ser 945 950 955 960 Ala Thr Gly Val Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn 965 970 975 Lys Ser Glu Lys Gln Asn Lys Gln Gln Lys Gln Ser Asp Thr Gln Lys 980 985 990 Lys Glu Tyr Gln Lys Asp Gln Gly Gly Asn Gly Leu Ser Ser Asp Gly 995 1000 1005 Ser Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Ala Lys Lys Glu Glu Asn Leu Ser Asp Trp Phe Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Thr Phe Phe Ile Trp Glu Thr Ile Lys Asp Phe Phe Asp 1060 1065 1070 Gly Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe 1075 1080 1085 Val Ser Gln Ala Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe 1090 1095 1100 Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala 1105 1110 1115 1120 Glu Pro Ile Ala Val Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala 1125 1130 1135 Phe Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Lys Leu Asn 1140 1145 1150 Gln Trp Cys Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe 1155 1160 1165 Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Thr Ala Phe Ala 1170 1175 1180 Thr Tyr Glu Asp Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr 1185 1190 1195 1200 Ala Arg Val Tyr Glu Glu Leu Leu Ala Ile Pro Ile Val Lys Gly Arg 1205 1210 1215 Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val 1220 1225 1230 Glu Ala Tyr Val Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser 1235 1240 1245 His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Val Phe Glu 1250 1255 1260 Asp Pro Lys Ile Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp 1265 1270 1275 1280 Gly Ile Ser Thr Arg Thr Ile Gly Val Met Thr Leu Ile His Gly Asp 1285 1290 1295 Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Val 1300 1305 1310 Ile Ile Pro Cys Gly Ile Thr Asn Ser Leu Ser Asp Glu Asp Lys Glu 1315 1320 1325 Ala Leu Met Lys Lys Cys Asn Glu Tyr Leu Lys Arg Leu Leu Arg Val 1330 1335 1340 Asn Val Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp 1345 1350 1355 1360 Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val 1365 1370 1375 Gly Pro Arg Asp Met Lys Asn Gly Gln Phe Val Ala Val Arg Arg Asp 1380 1385 1390 Thr Gly Gln Lys Met Thr Phe Ala Glu His Glu Ala Glu Asp Lys Leu 1395 1400 1405 Arg His Ile Leu Glu Glu Ile His Ala Asn Leu Tyr Asn Arg Ala Phe 1410 1415 1420 Glu Asp Leu Thr Asn His Met Val Val Ala Asn Thr Met Glu Asp Phe 1425 1430 1435 1440 Gln Lys Glu Leu Glu Ala Gly Lys Ile Val Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Met Glu Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln 1460 1465 1470 Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Lys Pro Leu Arg Glu Leu Gln Arg Gly Ala Lys Cys Val Cys 1490 1495 1500 Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 122 <211> 1519 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_009094640.1 <400> 122 Met Ala Ala Leu Ser Leu Thr Val Asp Ala Gly Ser Pro Pro Leu Gly 1 5 10 15 Ala Leu Leu Thr Val Glu His Val Lys Asn Asp Val Glu Ile Ser Val 20 25 30 Gln Glu Gly Lys Glu Thr Ile Leu Ser Val Ser Glu Gln Val Ser Phe 35 40 45 Thr Asp Val Asn Ser Ile Ala Arg Tyr Leu Ala Arg Val Ala Ala Ser 50 55 60 Ala Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His 65 70 75 80 Trp Leu Glu Phe Ser Ala Thr Lys Leu Ser Thr Ala Ser Gln Phe Pro 85 90 95 Ser Ala Ile Gln Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu 100 105 110 Val Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Val Leu 115 120 125 Lys Asp Ser Ser Thr Trp Gln Gly Gln Leu Glu Gln Asn Lys Ala Pro 130 135 140 Val His Ala Lys Arg Trp Tyr Ser Phe Leu Glu Val Gln Arg Ala Phe 145 150 155 160 Gln Ser Val Gly Ala Lys Trp Ala Ala Gly Thr Pro Lys Val Lys Met 165 170 175 Ala Thr Glu Lys Glu Lys Lys Ala Asp Val Gly Lys Phe Val Glu Leu 180 185 190 Pro Gly Ala Glu Met Gly Lys Val Ile Val Arg Phe Pro Pro Glu Ala 195 200 205 Ser Gly Tyr Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln 210 215 220 His Tyr Gln Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp 225 230 235 240 Thr Asn Pro Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu 245 250 255 Asp Val Ala Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser 260 265 270 Asp His Phe Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu 275 280 285 Gly Lys Ala Tyr Val Asp Asp Thr Pro Ala Glu Gln Met Lys Ala Glu 290 295 300 Arg Glu Gln Arg Val Glu Ser Lys His Arg Asn Asn Cys Val Asn Lys 305 310 315 320 Asn Leu Gln Met Trp Glu Glu Met Lys Lys Gly Thr Glu Tyr Gly Gln 325 330 335 Thr Cys Cys Leu Arg Ala Lys Ile Asp Met Ser Ser Asn Asn Gly Cys 340 345 350 Met Arg Asp Pro Thr Leu Tyr Arg Cys Lys Asn Gln Pro His Pro Arg 355 360 365 Thr Gly Ser Thr Tyr Asn Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro 370 375 380 Ile Val Asp Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu 385 390 395 400 Tyr His Asp Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly 405 410 415 Ile Arg Lys Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn 420 425 430 Thr Val Leu Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu 435 440 445 Val Asp Gly Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu 450 455 460 Arg Arg Gly Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln 465 470 475 480 Gly Ser Ser Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ser 485 490 495 Phe Asn Lys Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Thr Ala Leu 500 505 510 Leu Lys Asp Ala Val Val Pro Val Asn Ile Pro Glu Ala Gln Glu Glu 515 520 525 Met Lys Glu Val Ala Lys His Pro Lys Asn Ala Asp Val Gly Leu Lys 530 535 540 Pro Val Trp Tyr Ser Ser Lys Val Leu Ile Glu Gly Ala Asp Ala Glu 545 550 555 560 Thr Leu Ala Glu Gly Glu Val Val Thr Phe Ile Asn Trp Gly Asn Ile 565 570 575 Ile Ile Thr Lys Leu Asn Arg Asn Ser Ser Gly Lys Ile Val Ser Ile 580 585 590 Asn Ala Lys Leu Asn Leu Asp Asn Lys Asp Phe Lys Lys Thr Thr Lys 595 600 605 Ile Thr Trp Leu Ala Glu Thr Pro Arg Ala Pro Leu Ile Pro Thr Val 610 615 620 Cys Val Asn Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp 625 630 635 640 Glu Asp Phe Lys Gln Tyr Ile Asn Arg Asn Ser Lys Gln Glu Glu Leu 645 650 655 Met Leu Gly Asp Pro Cys Leu Lys Glu Leu Lys Lys Gly Asp Ile Ile 660 665 670 Gln Leu Gln Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro 675 680 685 Val Ser Pro Tyr Ser Cys Lys Asp Ala Pro Cys Ile Leu Ile Tyr Ile 690 695 700 Pro Asp Gly His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys 705 710 715 720 Thr Lys Ala Glu Thr Ala Lys Lys Glu Ala Ser Ser Ala Val Lys Glu 725 730 735 Lys Ser Ala Pro Val Val Gly Arg Thr Ser Thr Pro Ala Cys Ala Glu 740 745 750 Ser Ser Glu Gly Leu Leu Ile Ile Tyr Lys Lys Val Ala Ala Gln Gly 755 760 765 Asp Val Val Arg Asp Leu Lys Ala Lys Lys Ala Ala Lys Glu Asp Ile 770 775 780 Asp Lys Ala Val Lys Gln Leu Leu Ala Leu Lys Ala Glu Tyr Lys Glu 785 790 795 800 Lys Thr Gly Gln Glu Tyr Lys Pro Gly Asn Pro Pro Val Ser Gly Thr 805 810 815 Ala Gln Ser Ser Lys Leu Glu Thr Ser Gly Thr Leu Glu Ser Lys Ala 820 825 830 Leu Tyr Asp Lys Val Ala Glu Gln Gly Glu Val Val Arg Lys Leu Lys 835 840 845 Ala Glu Lys Ala Ser Lys Asp Gln Ile Asp Glu Ala Val Lys Leu Leu 850 855 860 Leu Ser Leu Lys Ala Asp Tyr Lys Glu Lys Thr Gly Gln Glu Tyr Lys 865 870 875 880 Pro Gly His Pro Pro Ala Ala Gln Gly Ala Leu Pro Gln Thr Thr Asn 885 890 895 Thr Val Pro Ser Gly Pro Asp Thr Pro Glu Ala Lys Ala Leu Phe Gly 900 905 910 Lys Val Ala Leu Gln Gly Asp Glu Val Arg Lys Leu Lys Ala Glu Lys 915 920 925 Ala Asp Lys Glu Lys Ile Asp Val Ala Val Lys Glu Leu Leu Gln Leu 930 935 940 Lys Ala Gln Tyr Lys Ser Val Ala Gly Val Asp Tyr Lys Pro Val Ser 945 950 955 960 Ala Ser Gly Val Asp Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn 965 970 975 Lys Ser Glu Lys Gln Ser Lys Gln Gln Lys Gln Asn Asp Gly Pro Lys 980 985 990 Lys Glu Pro Leu Gln Gly Gln Ser Gly Asn Glu Arg Ser Ser Asn Gly 995 1000 1005 Ser Gly Glu Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu 1010 1015 1020 Val Lys Lys Glu Glu Asn Leu Ser Glu Trp Phe Ser Gln Val Ile Thr 1025 1030 1035 1040 Lys Ser Glu Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Ile Leu 1045 1050 1055 Arg Pro Trp Ala His Ala Ile Trp Glu Ala Ile Lys Asp Phe Phe Asp 1060 1065 1070 Ala Glu Ile Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe 1075 1080 1085 Val Ser Gln Ala Ala Leu Glu Thr Glu Lys Ser His Ile Ala Asp Phe 1090 1095 1100 Ala Pro Glu Val Ala Trp Val Thr Arg Ser Gly Lys Thr Asp Leu Ala 1105 1110 1115 1120 Glu Pro Ile Ala Ile Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ser 1125 1130 1135 Tyr Ala Lys Trp Val Gln Ser His Arg Asp Leu Pro Ile Lys Leu Asn 1140 1145 1150 Gln Trp Cys Ser Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe 1155 1160 1165 Leu Arg Thr Arg Glu Phe Leu Trp Gln Glu Gly His Thr Ala Phe Ala 1170 1175 1180 Thr Tyr Glu Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr 1185 1190 1195 1200 Ala Arg Val Tyr Glu Asp Leu Leu Ala Ile Pro Val Val Lys Gly Arg 1205 1210 1215 Lys Thr Glu Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Leu 1220 1225 1230 Glu Ala Phe Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser 1235 1240 1245 His His Leu Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Val Phe Glu 1250 1255 1260 Asp Pro Lys Lys Pro Gly Glu Lys Gln Phe Ala Tyr Gln Asn Ser Trp 1265 1270 1275 1280 Gly Ile Thr Thr Arg Thr Ile Gly Val Met Thr Met Ile His Gly Asp 1285 1290 1295 Asn Met Gly Leu Val Leu Pro Pro Arg Val Ala Cys Ile Gln Val Val 1300 1305 1310 Ile Ile Pro Cys Gly Ile Thr Asn Ser Leu Ser Glu Glu Asp Arg Glu 1315 1320 1325 Ala Leu Leu Lys Lys Cys Asn Glu Tyr Arg Ser Arg Leu Leu Ala Val 1330 1335 1340 Asn Ile Arg Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp 1345 1350 1355 1360 Lys Phe Asn His Trp Glu Leu Lys Gly Val Pro Val Arg Val Glu Val 1365 1370 1375 Gly Pro Arg Asp Met Lys Ser Gln Gln Phe Val Ala Val Arg Arg Asp 1380 1385 1390 Thr Gly Gln Lys Leu Thr Leu Ser Glu His Glu Ala Glu Glu Lys Leu 1395 1400 1405 Lys Gln Ile Leu Glu Glu Ile Gln Ala Asn Leu Tyr Ser Arg Ala Ser 1410 1415 1420 Glu Asp Leu Lys Ser His Met Val Val Ala Ser Asn Met Glu Asp Phe 1425 1430 1435 1440 Gln Lys Glu Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys Gly 1445 1450 1455 Glu Ile Glu Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln 1460 1465 1470 Asp Leu Glu Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile 1475 1480 1485 Pro Phe Gln Pro Leu Arg Glu Leu Gln Pro Gly Ala Arg Cys Val Cys 1490 1495 1500 Gly Lys Asn Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 123 <211> 1516 <212> PRT <213> Artificial Sequence <220> <223> EPRS XP_006022406.1 <400> 123 Met Ala Leu Ser Leu Thr Val Asn Val Gly Asn Pro Pro Leu Gly Ala 1 5 10 15 Leu Leu Thr Val Glu His Val Lys Asn Asp Phe Glu Ile Leu Val Glu 20 25 30 Glu Gly Lys Glu Thr Val Leu Arg Ile Ser Asp His Val Thr Phe Thr 35 40 45 Asp Ala Asn Ser Ile Ala Arg Tyr Leu Ala Arg Val Ala Thr Ser Ala 50 55 60 Gly Leu Tyr Gly Ser Asn Leu Leu Glu His Thr Glu Ile Asp His Trp 65 70 75 80 Leu Glu Phe Ser Ala Thr Lys Leu Ser Ala Pro Ser Gln Phe Pro Ser 85 90 95 Ala Ile His Glu Leu Asn His Cys Leu Ser Leu Arg Thr Tyr Leu Val 100 105 110 Gly Asn Ser Leu Ser Leu Ala Asp Leu Cys Val Trp Ala Val Leu Lys 115 120 125 Gly Ser Asn Ile Trp Gln Glu Glu Leu Gln Gln Asn Lys Ala Pro Val 130 135 140 His Val Lys Arg Trp Tyr Asp Phe Leu Glu Ala Gln Cys Ala Phe Gln 145 150 155 160 Ser Val Gly Thr Lys Trp Ala Ala Ala Thr Pro Lys Thr Asn Val Ala 165 170 175 Thr Glu Lys Lys Gln Asp Val Gly Lys Phe Val Glu Leu Pro Gly Ala 180 185 190 Glu Val Gly Lys Val Ile Val Arg Phe Pro Pro Glu Ala Ser Gly Tyr 195 200 205 Leu His Ile Gly His Ala Lys Ala Ala Leu Leu Asn Gln His Tyr Gln 210 215 220 Val Asn Phe Lys Gly Lys Leu Ile Met Arg Phe Asp Asp Thr Asn Pro 225 230 235 240 Glu Lys Glu Lys Glu Asp Phe Glu Lys Val Ile Leu Glu Asp Val Ala 245 250 255 Met Leu His Ile Lys Pro Asp Gln Phe Thr Tyr Thr Ser Asp His Phe 260 265 270 Glu Thr Ile Met Lys Tyr Ala Glu Lys Leu Ile Gln Glu Gly Lys Ala 275 280 285 Tyr Val Asp Asn Thr Pro Ala Glu Gln Met Lys Met Glu Arg Glu Gln 290 295 300 Arg Lys Glu Ser Lys His Arg Asn Asn Ser Val Glu Lys Asn Leu Glu 305 310 315 320 Met Trp Glu Glu Met Lys Lys Gly Thr Glu Tyr Gly Gln Thr Cys Cys 325 330 335 Leu Arg Ala Lys Ile Asp Met Ser Cys Asn Asn Gly Cys Met Arg Asp 340 345 350 Pro Thr Leu Tyr Arg Cys Lys Asn Gln Pro His Pro Arg Thr Gly Asn 355 360 365 Thr Tyr Lys Val Tyr Pro Thr Tyr Asp Phe Ala Cys Pro Ile Val Asp 370 375 380 Ser Ile Glu Gly Val Thr His Ala Leu Arg Thr Thr Glu Tyr His Asp 385 390 395 400 Arg Asp Glu Gln Phe Tyr Trp Ile Ile Glu Ala Leu Gly Ile Arg Lys 405 410 415 Pro Tyr Ile Trp Glu Tyr Ser Arg Leu Asn Leu Asn Asn Thr Val Leu 420 425 430 Ser Lys Arg Lys Leu Thr Trp Phe Val Asn Glu Gly Leu Val Asp Gly 435 440 445 Trp Asp Asp Pro Arg Phe Pro Thr Val Arg Gly Val Leu Arg Arg Gly 450 455 460 Met Thr Val Glu Gly Leu Lys Gln Phe Ile Ala Ala Gln Gly Ser Ser 465 470 475 480 Arg Ser Val Val Asn Met Glu Trp Asp Lys Ile Trp Ala Phe Asn Lys 485 490 495 Lys Val Ile Asp Pro Val Ala Pro Arg Tyr Thr Ala Leu Leu Lys Asp 500 505 510 Asp Val Val Thr Val Asn Ile Pro Glu Ala Val Glu Glu Met Lys Glu 515 520 525 Val Ala Lys His Pro Lys Asn Pro Asp Val Gly Leu Lys Pro Val Trp 530 535 540 Tyr Gly Ser Arg Val Leu Ile Glu Gly Ala Asp Ala Asp Thr Leu Thr 545 550 555 560 Glu Gly Glu Met Val Thr Phe Ile Asn Trp Gly Asn Ile Ile Ile Thr 565 570 575 Lys Ile His Arg Asn Ser Asn Arg Lys Ile Val Ser Ile Asp Ala Lys 580 585 590 Leu Asn Leu Glu Asn Lys Asp Tyr Lys Lys Thr Ala Lys Ile Thr Trp 595 600 605 Leu Thr Asn Thr Pro Ser Ala Pro Leu Ile Pro Ser Val Cys Val Asn 610 615 620 Tyr Glu His Leu Ile Thr Lys Pro Val Leu Gly Lys Asp Glu Asp Phe 625 630 635 640 Lys Gln Tyr Val Asn Arg Asn Ser Lys Gln Glu Glu Leu Met Leu Gly 645 650 655 Asp Pro Cys Leu Lys Glu Leu Lys Lys Gly Asp Ile Ile Gln Leu Gln 660 665 670 Arg Arg Gly Phe Phe Ile Cys Asp Gln Pro Tyr Glu Pro Ile Ser Pro 675 680 685 Tyr Ser Cys Lys Glu Ala Pro Cys Ile Leu Ile Tyr Ile Pro Asp Gly 690 695 700 His Thr Lys Glu Met Pro Thr Ser Gly Ser Lys Glu Lys Thr Lys Ala 705 710 715 720 Glu Thr Ala Lys Lys Glu Ala Ser Ser Ala Ser Lys Glu Lys Pro Thr 725 730 735 Pro Pro Phe Val Ala Asp Thr Ser Thr Ala Pro Ala Asp Pro Val Val 740 745 750 Leu Tyr Asn Arg Val Ser Ala Gln Gly Asp Val Val Arg Asp Leu Lys 755 760 765 Ala Lys Lys Ala Ser Lys Glu Asp Ile Asp Ala Ala Val Lys Gln Leu 770 775 780 Leu Ala Leu Lys Ala Glu Tyr Arg Glu Lys Thr Gly Gln Glu Tyr Lys 785 790 795 800 Pro Gly Ser His Pro Ala Ala Ala Ala Ser Val Pro Ser Val Pro Ser 805 810 815 Asn Leu Glu Thr Ser Ser Met Leu Asp Ser Thr Thr Leu Tyr Asp Lys 820 825 830 Val Ala Glu Gln Gly Asp Met Val Arg Lys Leu Lys Ala Glu Arg Ala 835 840 845 Pro Lys Asp Lys Val Asp Glu Ala Val Arg Leu Leu Leu Ser Leu Lys 850 855 860 Ala Glu Tyr Lys Glu Lys Thr Gly Gln Asp Tyr Lys Pro Gly His Pro 865 870 875 880 Pro Ala Ala Gln Thr Ser Ala Ser Pro Gln Ala Thr Ser Ile Glu Thr 885 890 895 Ser Gly Ser Asp Thr Pro Glu Ala Lys Ala Leu Tyr Asp Lys Val Ala 900 905 910 Leu Gln Gly Glu Val Val Arg Lys Leu Lys Ala Glu Lys Ala Glu Lys 915 920 925 Asp Lys Ile Asp Ala Ala Val Gln Glu Leu Leu Gln Leu Lys Ala Gln 930 935 940 Tyr Lys Ser Phe Ala Gly Val Asp Tyr Lys Pro Ala Ser Met Thr Gly 945 950 955 960 Ile Glu Asp Lys Asp Lys Lys Lys Lys Glu Lys Glu Asn Lys Ser Glu 965 970 975 Lys Gln Asn Lys Gln Gln Lys Gln Asn Glu Thr Pro Lys Lys Glu Ser 980 985 990 Pro Lys Glu Gln Cys Ala Asn Asp Leu Ser Ser Asn Gly Ala Gly Glu 995 1000 1005 Gly Gln Gly Pro Lys Lys Gln Thr Arg Leu Gly Leu Glu Ala Lys Lys 1010 1015 1020 Glu Glu Asn Leu Ala Asp Trp Phe Ser Gln Val Ile Thr Lys Ser Glu 1025 1030 1035 1040 Met Ile Glu Tyr Tyr Asp Val Ser Gly Cys Tyr Val Leu Arg Pro Trp 1045 1050 1055 Ala Tyr Ala Ile Trp Glu Ala Ile Arg Asp Phe Phe Asp Thr Glu Ile 1060 1065 1070 Lys Lys Leu Gly Val Glu Asn Cys Tyr Phe Pro Met Phe Val Ser Gln 1075 1080 1085 Ala Ala Leu Glu Lys Glu Lys Thr His Ile Ala Asp Phe Ala Pro Glu 1090 1095 1100 Val Ala Trp Val Thr Arg Ser Gly Lys Thr Glu Leu Ala Glu Pro Ile 1105 1110 1115 1120 Ala Val Arg Pro Thr Ser Glu Thr Val Met Tyr Pro Ala Tyr Ala Lys 1125 1130 1135 Trp Val Gln Ser His Arg Asp Leu Pro Ile Lys Leu Asn Gln Trp Cys 1140 1145 1150 Asn Val Val Arg Trp Glu Phe Lys His Pro Gln Pro Phe Leu Arg Thr 1155 1160 1165 Arg Glu Phe Leu Trp Gln Glu Gly His Thr Ala Phe Ala Thr Tyr Glu 1170 1175 1180 Glu Ala Ala Glu Glu Val Leu Gln Ile Leu Asp Leu Tyr Ala Arg Val 1185 1190 1195 1200 Tyr Glu Glu Phe Leu Ala Ile Pro Val Val Lys Gly Arg Lys Thr Glu 1205 1210 1215 Lys Glu Lys Phe Ala Gly Gly Asp Tyr Thr Thr Thr Val Glu Ala Tyr 1220 1225 1230 Ile Ser Ala Ser Gly Arg Ala Ile Gln Gly Ala Thr Ser His His Leu 1235 1240 1245 Gly Gln Asn Phe Ser Lys Met Phe Glu Ile Val Phe Glu Asp Pro Lys 1250 1255 1260 Lys Pro Gly Glu Lys Gln Leu Ala Tyr Gln Asn Ser Trp Gly Ile Thr 1265 1270 1275 1280 Thr Arg Thr Ile Gly Val Met Thr Met Ile His Gly Asp Asn Met Gly 1285 1290 1295 Leu Val Leu Pro Pro Arg Val Ala Cys Val Gln Val Ile Ile Ile Pro 1300 1305 1310 Cys Gly Ile Thr Asn Ser Leu Ser Glu Glu Asp Lys Asp Ala Leu Leu 1315 1320 1325 Lys Lys Cys Asn Asp Tyr Leu Lys Arg Leu Leu Ser Val Asn Ile Arg 1330 1335 1340 Val Arg Ala Asp Leu Arg Asp Asn Tyr Ser Pro Gly Trp Lys Phe Asn 1345 1350 1355 1360 His Trp Glu Leu Lys Gly Val Pro Ile Arg Leu Glu Val Gly Pro Arg 1365 1370 1375 Asp Met Lys Asn His Gln Phe Val Ala Val Arg Arg Asp Thr Gly Gln 1380 1385 1390 Lys Leu Thr Phe Ala Glu Asp Glu Thr Glu Asp Lys Leu Ser Gln Ile 1395 1400 1405 Leu Glu Glu Ile His Ala Asn Leu Tyr Asn Arg Ala Phe Glu Asp Leu 1410 1415 1420 Lys Asn His Met Val Val Ser Asn Asp Met Asp Asp Phe Gln Lys Glu 1425 1430 1435 1440 Leu Asp Ser Gly Lys Ile Val Gln Ile Pro Phe Cys Gly Glu Ile Glu 1445 1450 1455 Cys Glu Asp Trp Ile Lys Lys Thr Thr Ala Arg Asp Gln Asp Leu Glu 1460 1465 1470 Pro Gly Ala Pro Ser Met Gly Ala Lys Ser Leu Cys Ile Pro Phe Lys 1475 1480 1485 Pro Leu Cys Glu Leu Gln Ala Gly Thr Lys Cys Val Cys Gly Lys Asn 1490 1495 1500 Pro Ala Lys Phe Tyr Thr Leu Phe Gly Arg Ser Tyr 1505 1510 1515 <210> 124 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 AAD17332.1 <400> 124 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Leu Gln 65 70 75 80 Glu Ser Pro Arg Val Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Glu 130 135 140 Ala Lys Asp Leu Gln Ala Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Cys Pro Arg 260 <210> 125 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 BAG36039.1 <400> 125 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Leu Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Leu Gln 65 70 75 80 Glu Ser Pro Arg Val Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Val Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Glu 130 135 140 Ala Lys Asp Leu Gln Ala Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Cys Pro Arg 260 <210> 126 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004062397.1 <400> 126 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Glu 130 135 140 Ala Lys Asp Leu Gln Ala Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Cys Pro Arg 260 <210> 127 <211> 172 <212> PRT <213> Artificial Sequence <220> <223> Snail1 AAF32527.1 <400> 127 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Leu Gln 65 70 75 80 Glu Ser Pro Arg Val Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Val Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Glu 130 135 140 Ala Lys Asp Leu Gln Ala Arg Lys Ala Ser Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His 165 170 <210> 128 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_003809303.1 <400> 128 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Asp 130 135 140 Ala Lys Asp Leu Gln Ala Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Cys Pro Arg 260 <210> 129 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_003252972.1 <400> 129 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Lys Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Glu 130 135 140 Ala Lys Asp Leu Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Cys Pro Arg 260 <210> 130 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_010382148.1 <400> 130 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Ser Pro Arg Val Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Thr Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Thr Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Glu 130 135 140 Ala Lys Asp Leu Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Cys Pro Arg 260 <210> 131 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_001097698.1 <400> 131 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Thr Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Glu 130 135 140 Ala Lys Asp Leu Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Cys Pro Arg 260 <210> 132 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_002830458.1 <400> 132 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Glu 130 135 140 Ala Lys Asp Leu Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Xaa Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Cys Pro Arg 260 <210> 133 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_009435687.1 <400> 133 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Arg Gly Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Asp 130 135 140 Ala Lys Asp Leu Gln Ala Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Cys Pro Arg 260 <210> 134 <211> 265 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_003932616.1 <400> 134 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ser Leu Arg Arg Pro 65 70 75 80 Gln Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp 85 90 95 Ser Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Ser Ser 100 105 110 Ser Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala 115 120 125 Thr Phe Pro Gly Leu Val Gln Val Pro Lys Gln Leu Ala Gln Leu Ser 130 135 140 Glu Ala Lys Asp Leu Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys 145 150 155 160 Asn Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser 165 170 175 His Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg 180 185 190 Pro Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro 195 200 205 Phe Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu 210 215 220 Arg Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln 225 230 235 240 Ala Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln 245 250 255 Glu Ser Gly Cys Ser Gly Cys Pro Arg 260 265 <210> 135 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_003787739.1 <400> 135 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Val Gln Pro Ile Gly Trp Ala Ser Phe Arg Pro Gln 65 70 75 80 Glu Ser Pro Lys Val Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Thr 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Val Gln Leu Ser Glu 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 136 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_008060134.1 <400> 136 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Asn Leu Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Ile Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ala Trp Ala Ala Leu Arg Pro Gln 65 70 75 80 Glu Ser Thr Lys Val Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Pro Arg Leu Ala Arg Leu Ser Glu 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asp 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Pro Ala 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 137 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_008570069.1 <400> 137 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ser Gly Ser Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Ser Leu Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Ser Pro Lys Val Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Ala Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Glu 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 138 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004883645.1 <400> 138 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Leu Ala Gln Pro Leu Gly Trp Ala Ser Leu Gln Pro Gln 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Thr Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Val 130 135 140 Ala Lys Asp Leu Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Met 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Leu Gly Gly Pro Arg 260 <210> 139 <211> 263 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_003732892.1 <400> 139 Met Pro Arg Ser Phe Ser Cys Gln Lys Pro Xaa Asp Ser Asn Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Thr Leu Ser Lys Phe Thr Phe Gln Gln 20 25 30 Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu Ile 35 40 45 Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Val Leu 50 55 60 Ala Pro Gln Ala Gln Pro Thr Ala Trp Ala Ser Leu Arg Ala Gln Glu 65 70 75 80 Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser Gly 85 90 95 Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Ser Ser Ser Phe 100 105 110 Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Val Phe 115 120 125 Pro Gly Leu Val Gln Val Pro Lys Gln Leu Ala Gln Leu Ser Glu Ala 130 135 140 Lys Asp Leu Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn Lys 145 150 155 160 Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His Thr 165 170 175 Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro Trp 180 185 190 Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe Ser 195 200 205 Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg Ala 210 215 220 His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala Cys 225 230 235 240 Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu Ser 245 250 255 Gly Cys Ser Gly Cys Pro Arg 260 <210> 140 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 KFO35879.1 <400> 140 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Ser Pro Glu Leu Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ser Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Leu Ala Gln Pro Leu Cys Trp Ala Ser Leu Gln Pro Gln 65 70 75 80 Glu Ser Pro Lys Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Ala Ser Thr Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Ala 130 135 140 Ala Lys Asp Leu Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Asp Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Arg Met 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 141 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_003467769.1 <400> 141 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Ser Pro Glu Leu Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Leu Gly Gln Pro Leu Gly Trp Ala Ser Leu Gln Pro Gln 65 70 75 80 Glu Ser Pro Lys Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Ala Ser Thr Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gln Leu Ser Met 130 135 140 Ala Lys Asp Leu Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Ser 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Glu Val Lys Lys Tyr Gln Cys Arg Thr 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Leu Gly Arg Pro Arg 260 <210> 142 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_006141336.1 <400> 142 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ser Gly Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Ser Leu Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ser Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Val Gln Pro Val Gly Trp Ala Ser Leu Gln Pro Gln 65 70 75 80 Glu Gly Pro Lys Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Ser Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gln Leu Ser Met 130 135 140 Ala Lys Asp Ser Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Glu Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 143 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_005325275.1 <400> 143 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Val Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Cys Leu 50 55 60 Leu Ala Pro His Thr Gln Pro Ile Gly Trp Ala Ser Leu Gln Pro Gln 65 70 75 80 Glu Asn Pro Gln Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ile Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Leu Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Pro Arg 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 144 <211> 510 <212> PRT <213> Artificial Sequence <220> <223> Snail1 ELW71708.1 <400> 144 Met Ala Ala Gln His Pro Gly Arg Asp Gly Gly Ala Gln Leu Ala Gly 1 5 10 15 Ala Ala Thr Glu Ala Asp Pro Leu Gly Arg Phe Thr Cys Pro Val Cys 20 25 30 Leu Glu Val Tyr Glu Lys Pro Gly Gln Val Pro Cys Gly His Gly Phe 35 40 45 Cys Ser Ala Cys Leu Gln Glu Cys Leu Lys Pro Lys Lys Pro Val Cys 50 55 60 Gly Val Cys Arg Ser Ala Leu Ala Pro Gly Val Arg Ala Ala Glu Leu 65 70 75 80 Glu Arg Gln Ile Glu Ser Thr Glu Thr Ser Cys His Gly Cys Arg Lys 85 90 95 Asn Phe Phe Leu Ser Lys Ile Arg Ala His Val Ala Thr Cys Ser Lys 100 105 110 Tyr Gln Asn Tyr Ile Met Glu Gly Val Lys Ala Thr Thr Lys Asp Ala 115 120 125 Ser Leu Gln Ala Arg Asn Val Pro Asn Arg Tyr Thr Phe Pro Cys Pro 130 135 140 Tyr Cys Pro Glu Lys Asn Phe Asp Gln Glu Gly Leu Val Glu His Cys 145 150 155 160 Lys Leu Ala His Ser Met Asp Thr Lys Ser Val Val Cys Pro Ile Cys 165 170 175 Ala Ser Met Pro Trp Gly Asp Pro Asn Tyr Arg Ser Ala Asn Phe Ile 180 185 190 Glu His Ile Gln Arg Arg His Gln Phe Ser Tyr Asp Thr Phe Val Asp 195 200 205 Tyr Glu Val Asp Glu Glu Asp Met Met Asn Gln Val Leu Gln Arg Ser 210 215 220 Ile Ile Asp Gln Trp Asn Ala Leu Arg Pro Pro Arg Asp Pro Ala Thr 225 230 235 240 Pro Ala Gly Ser Val Thr Met Pro Arg Ser Phe Leu Val Arg Lys Ser 245 250 255 Ser Gly Pro Arg Arg Lys Pro Asn Tyr Ser Glu Leu Gln Asp Ser Ser 260 265 270 Leu Glu Phe Thr Phe Gln Gln Pro Tyr Asp Gln Ala His Leu Leu Ala 275 280 285 Ala Ile Pro Pro Pro Glu Val Leu Asn Pro Ser Ala Ser Leu Pro Met 290 295 300 Leu Ile Trp Asp Ser Leu Leu Ala Pro Gln Val Gln Pro Val Gly Trp 305 310 315 320 Ala Ser Leu Gln Pro Gln Glu Gly Pro Lys Ala Ala Glu Leu Thr Ser 325 330 335 Leu Ser Asp Glu Asp Ser Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro 340 345 350 Ser Pro Ala Ser Ser Ser Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu 355 360 365 Ala Glu Ala Tyr Ala Ala Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln 370 375 380 Leu Ala Gln Leu Ser Met Ala Lys Asp Ser Gln Ser Arg Lys Ala Phe 385 390 395 400 Asn Cys Lys Tyr Cys Asn Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys 405 410 415 Met His Ile Arg Ser His Thr Leu Pro Cys Val Cys Gly Thr Cys Gly 420 425 430 Lys Ala Phe Ser Arg Pro Trp Leu Leu Gln Gly His Val Arg Thr His 435 440 445 Thr Gly Glu Lys Pro Phe Ser Cys Ser His Cys Ser Arg Ala Phe Ala 450 455 460 Asp Arg Ser Asn Leu Arg Ala His Leu Gln Thr His Ser Asp Val Lys 465 470 475 480 Lys Tyr Gln Cys Lys Thr Cys Ala Arg Thr Phe Ser Arg Met Ser Leu 485 490 495 Leu His Lys His Glu Glu Ser Gly Cys Ser Gly Gly Pro Arg 500 505 510 <210> 145 <211> 237 <212> PRT <213> Artificial Sequence <220> <223> Snail1 EFB26680.1 <400> 145 Glu Phe Thr Phe Gln Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala 1 5 10 15 Ile Pro Pro Pro Glu Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu 20 25 30 Ile Trp Asp Ser Leu Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala 35 40 45 Ser Leu Leu Pro Gln Glu Val Pro Lys Val Val Glu Leu Thr Ser Leu 50 55 60 Ser Asp Glu Asp Ser Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser 65 70 75 80 Pro Ala Pro Ser Ser Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala 85 90 95 Glu Gly Tyr Val Ala Phe Pro Gly Leu Gly Gln Leu Pro Thr Gln Leu 100 105 110 Ala Gly Leu Ser Val Ala Lys Asp Ser Gln Thr Arg Lys Ala Phe Asn 115 120 125 Cys Lys Tyr Cys Asn Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met 130 135 140 His Ile Arg Ser His Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys 145 150 155 160 Ala Phe Ser Arg Pro Trp Leu Leu Gln Gly His Val Arg Thr His Thr 165 170 175 Gly Glu Lys Pro Phe Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp 180 185 190 Arg Ser Asn Leu Arg Ala His Leu Gln Thr His Ser Asp Val Lys Lys 195 200 205 Tyr Gln Cys Lys Thr Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu 210 215 220 His Lys His Gln Glu Ser Gly Cys Ser Gly Gly Pro Arg 225 230 235 <210> 146 <211> 242 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_008696422.1 <400> 146 Leu His Leu Pro Ser Glu Phe Thr Phe Gln Gln Pro Tyr Asp Gln Ala 1 5 10 15 His Leu Leu Ala Ala Ile Pro Pro Pro Glu Val Leu Asn Pro Thr Ala 20 25 30 Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu Leu Ala Pro Gln Ala Gln 35 40 45 Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln Glu Val Pro Lys Ala Val 50 55 60 Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser Gly Lys Gly Ser Gln Pro 65 70 75 80 Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser Phe Ser Ser Thr Ser Ala 85 90 95 Ser Ser Leu Glu Ala Glu Gly Tyr Val Ala Phe Pro Gly Leu Gly Gln 100 105 110 Leu Pro Thr Gln Leu Ala Gly Leu Ser Val Ala Lys Asp Ser Gln Thr 115 120 125 Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn Lys Glu Tyr Leu Ser Leu 130 135 140 Gly Ala Leu Lys Met His Ile Arg Ser His Thr Leu Pro Cys Val Cys 145 150 155 160 Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro Trp Leu Leu Gln Gly His 165 170 175 Val Arg Thr His Thr Gly Glu Lys Pro Phe Ser Cys Ser His Cys Ser 180 185 190 Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg Ala His Leu Gln Thr His 195 200 205 Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr Cys Ser Arg Thr Phe Ser 210 215 220 Arg Met Ser Leu Leu His Lys His Gln Glu Ser Gly Cys Ser Gly Gly 225 230 235 240 Pro Arg <210> 147 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 NP_446257.1 <400> 147 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ala Cys Val Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ala Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Val Ala Trp Ala Thr Leu Pro Leu Arg 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Ser Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Phe Ile Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Thr Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Arg Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 148 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_005392380.1 <400> 148 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Val 50 55 60 Leu Ala Pro Leu Thr Gln Pro Leu Gly Arg Ala Pro Val Gln Pro Gln 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Ala Ser Thr Ser Ser Leu Glu Ala Glu Ala Cys Ala Ala 115 120 125 Leu Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Thr Gly Leu Ser Met 130 135 140 Ala Lys Asp Leu Gln Cys Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Thr Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 149 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_008833387.1 <400> 149 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ala Cys Leu Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Val His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ala Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Val Ala Trp Ala Ser Leu Pro Pro Arg 65 70 75 80 Glu Ser Pro Lys Ala Thr Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Phe Val Ala 115 120 125 Phe Ser Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 150 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_005362911.1 <400> 150 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ala Cys Val Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ala Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Val Ala Trp Ala Thr Leu Pro Pro Arg 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Ser Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Phe Ile Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 151 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_006992689.1 <400> 151 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ala Cys Val Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ala Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Val Ala Trp Ala Thr Leu Pro Pro Arg 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Ser Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Phe Ile Ala 115 120 125 Phe Ser Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 152 <211> 272 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_005688790.1 <400> 152 Met Trp Arg Asn Arg Lys Lys Ala Val Asp Leu Cys Ala Gly Gly Gly 1 5 10 15 Ala Glu Arg Leu Lys Ile Ser Ala Ala Glu Val Ser Glu Leu Arg Asp 20 25 30 Ser Ser Pro Glu Leu Thr Phe Gln Gln Pro Tyr Asp Gln Ala His Leu 35 40 45 Leu Ala Ala Ile Pro Pro Pro Glu Val Leu Asn Pro Thr Ala Ser Leu 50 55 60 Pro Thr Leu Ile Trp Asp Ser Leu Leu Ala Pro Gln Ala Gln Pro Ile 65 70 75 80 Ser Trp Ala Ser Leu Leu Pro Gln Glu Ile Pro Lys Ala Gly Glu Leu 85 90 95 Thr Ser Leu Ser Asp Glu Asp Ser Gly Lys Gly Ser Gln Pro Pro Ser 100 105 110 Pro Pro Ser Pro Ala Pro Ser Ser Phe Ser Ser Thr Ser Ala Ser Ser 115 120 125 Leu Glu Ala Glu Gly Tyr Ala Ala Phe Pro Gly Leu Gly Gln Leu Pro 130 135 140 Lys Gln Leu Thr Gly Leu Ser Val Ala Lys Asp Pro Gln Ser Arg Lys 145 150 155 160 Ala Phe Asn Cys Lys Tyr Cys Asn Lys Glu Tyr Val Ser Leu Gly Ala 165 170 175 Leu Lys Met His Ile Arg Ser His Thr Leu Pro Cys Val Cys Ser Thr 180 185 190 Cys Gly Lys Ala Phe Ser Arg Pro Trp Leu Leu Gln Gly His Val Arg 195 200 205 Thr His Thr Gly Glu Lys Pro Phe Ser Cys Pro His Cys Ser Arg Ala 210 215 220 Phe Ala Asp Arg Ser Asn Leu Arg Ala His Leu Gln Thr His Ser Asp 225 230 235 240 Val Lys Lys Tyr Gln Cys Lys Thr Cys Ser Arg Thr Phe Ser Arg Met 245 250 255 Ser Leu Leu His Lys His Gln Glu Ser Gly Cys Ser Gly Gly Pro Arg 260 265 270 <210> 153 <211> 238 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_005635224.1 <400> 153 Ser Glu Phe Thr Phe Gln Gln Pro Tyr Asp Gln Ala His Leu Leu Ala 1 5 10 15 Ala Ile Pro Pro Pro Glu Val Leu Asn Pro Thr Ala Ser Leu Pro Thr 20 25 30 Leu Ile Trp Asp Ser Leu Leu Ala Pro Gln Ala Gln Pro Met Ser Trp 35 40 45 Ala Ala Leu Leu Pro Gln Glu Val Pro Lys Ala Val Glu Leu Thr Ser 50 55 60 Leu Ser Asp Glu Asp Ser Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro 65 70 75 80 Ser Pro Ala Pro Ser Ser Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu 85 90 95 Ala Glu Gly Tyr Val Ala Phe Pro Gly Leu Gly Gln Leu Pro Thr Gln 100 105 110 Leu Ala Gly Leu Ser Val Ala Lys Asp Ser Gln Thr Arg Lys Ala Phe 115 120 125 Asn Cys Lys Tyr Cys Asn Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys 130 135 140 Met His Ile Arg Ser His Thr Leu Pro Cys Val Cys Gly Thr Cys Gly 145 150 155 160 Lys Ala Phe Ser Arg Pro Trp Leu Leu Gln Gly His Val Arg Thr His 165 170 175 Thr Gly Glu Lys Pro Phe Ser Cys Ser His Cys Ser Arg Ala Phe Ala 180 185 190 Asp Arg Ser Asn Leu Arg Ala His Leu Gln Thr His Ser Asp Val Lys 195 200 205 Lys Tyr Gln Cys Lys Thr Cys Ser Arg Thr Phe Ser Arg Met Ser Leu 210 215 220 Leu His Lys His Gln Glu Ser Gly Cys Ser Gly Gly Pro Arg 225 230 235 <210> 154 <211> 244 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_008532217.1 <400> 154 Phe Leu Cys Leu Pro Ser Glu Phe Thr Phe Gln Gln Pro Tyr Asp Gln 1 5 10 15 Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu Val Leu Asn Pro Thr 20 25 30 Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu Leu Ala Pro Gln Ala 35 40 45 Gln Ala Ile Gly Trp Ala Ser Leu Leu Pro Gln Glu Met Pro Lys Ala 50 55 60 Val Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser Gly Lys Gly Ser 65 70 75 80 Gln Pro Pro Ser Pro Pro Ser Pro Thr Pro Ser Ser Phe Ser Ser Thr 85 90 95 Ser Ala Ser Ser Leu Glu Ala Glu Gly Phe Ala Ala Phe Pro Gly Leu 100 105 110 Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser Val Ala Lys Asp Pro 115 120 125 Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn Lys Glu Tyr Val 130 135 140 Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His Thr Leu Pro Cys 145 150 155 160 Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro Trp Leu Leu Gln 165 170 175 Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe Ser Cys Ser His 180 185 190 Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg Ala His Leu Gln 195 200 205 Thr His Ser Asp Val Lys Lys Tyr His Cys Lys Ala Cys Ala Arg Thr 210 215 220 Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu Ala Gly Cys Ser 225 230 235 240 Gly Gly Pro Arg <210> 155 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_002913027.1 <400> 155 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Val Pro Lys Val Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Val Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Thr Gln Leu Ala Gly Leu Ser Val 130 135 140 Ala Lys Asp Ser Gln Thr Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 156 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004687529.1 <400> 156 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Ala Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Ile Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Thr Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser Val 130 135 140 Val Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Asp Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 157 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004412574.1 <400> 157 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Val Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Thr Gln Leu Ala Gly Leu Ser Val 130 135 140 Ala Lys Asp Ser Gln Thr Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 158 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_007185370.1 <400> 158 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Val Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ser Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 159 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_007446447.1 <400> 159 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Ile Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ser Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 160 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_007128099.1 <400> 160 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Leu Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ser Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 161 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004282941.1 <400> 161 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Ile Pro Lys Ala Ile Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ser Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 162 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 NP_035557.1 <400> 162 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ala Cys Val Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ala Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Arg Pro Val Ala Trp Ala Thr Leu Pro Leu Arg 65 70 75 80 Glu Ser Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Ser Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Phe Ile Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ile Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Thr Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Arg Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 163 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004746271.1 <400> 163 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Val Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Val Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Thr Gln Leu Ala Gly Leu Ser Val 130 135 140 Ala Lys Asp Ser Gln Thr Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 164 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_006206723.1 <400> 164 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Ser Gln 65 70 75 80 Glu Ile Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Thr Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Thr Gly Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 165 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_006175044.1 <400> 165 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Ser Gln 65 70 75 80 Glu Ile Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ile Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Thr Gly Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 166 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 NP_001106179.1 <400> 166 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Gly Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Leu Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ser Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Ile Pro Lys Ala Gly Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Thr Gly Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ser Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 167 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004430338.1 <400> 167 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Ile Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Thr Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Ser 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Ser Ala 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ala Gly Gly Pro Arg 260 <210> 168 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_005074485.1 <400> 168 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Thr Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ala Cys Val Asp Phe Val Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ala Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Val Ala Trp Ala Thr Leu Pro Pro Arg 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Ser Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Phe Ile Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Thr Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr His Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 169 <211> 242 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_007608553.1 <400> 169 Pro Pro Leu Pro Ala Glu Phe Ile Phe Gln Gln Pro Tyr Asp Gln Ala 1 5 10 15 His Leu Leu Ala Ala Ile Pro Pro Pro Glu Val Leu Asn Pro Ala Ala 20 25 30 Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu Leu Val Pro Gln Val Gln 35 40 45 Pro Val Ala Trp Ala Thr Leu Pro Pro Arg Glu Ser Pro Arg Ala Ala 50 55 60 Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser Gly Lys Ser Ser Gln Pro 65 70 75 80 Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser Phe Ser Ser Thr Ser Ala 85 90 95 Ser Ser Leu Asp Ser Glu Ala Phe Val Ser Phe Pro Gly Leu Gly Gln 100 105 110 Leu Pro Lys Gln Leu Ala Arg Leu Ser Val Ala Lys Asp Pro Gln Ser 115 120 125 Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn Lys Glu Tyr Leu Ser Leu 130 135 140 Gly Ala Leu Lys Met His Ile Arg Ser His Thr Leu Pro Cys Val Cys 145 150 155 160 Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro Trp Leu Leu Gln Gly His 165 170 175 Val Arg Thr His Thr Gly Glu Lys Pro Phe Ser Cys Ser His Cys Asn 180 185 190 Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg Ala His Leu Gln Thr His 195 200 205 Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala Cys Ala Arg Thr Phe Ser 210 215 220 Arg Met Ser Leu Leu His Lys His Gln Glu Ser Gly Cys Ser Gly Gly 225 230 235 240 Pro Arg <210> 170 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_005983433.1 <400> 170 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Gly Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Leu Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Ser Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Ile Pro Lys Ala Gly Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Thr Gly Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Ser 145 150 155 160 Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ser Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 171 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 CAA47675.1 <400> 171 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ala Cys Val Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ala Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Arg Pro Val Ala Trp Ala Thr Leu Pro Leu Arg 65 70 75 80 Glu Ser Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Ser Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala Phe Ile Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ile Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Thr Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Arg Ala Phe Ala Val Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Arg Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 172 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_003983440.1 <400> 172 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ala Leu Leu Pro Gln 65 70 75 80 Glu Val Pro Lys Ala Ala Glu Leu Thr Ser Leu Ser Asp Asp Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Val Ala 115 120 125 Phe Pro Gly Leu Gly Arg Leu Pro Ala Gln Leu Ala Gly Leu Ser Val 130 135 140 Ala Lys Asp Ser Gln Thr Arg Lys Ala Phe Ser Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 173 <211> 265 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_001501267.2 <400> 173 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Ala Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Met Pro Lys Ala Val Val Glu Leu Thr Ser Leu Ser Asp Glu Asp 85 90 95 Ser Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Thr Pro Ser 100 105 110 Ser Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Phe Ala 115 120 125 Ala Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser 130 135 140 Val Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys 145 150 155 160 Asn Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser 165 170 175 His Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg 180 185 190 Pro Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro 195 200 205 Phe Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu 210 215 220 Arg Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr His Cys Lys 225 230 235 240 Ala Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln 245 250 255 Glu Ala Gly Cys Ser Gly Gly Pro Arg 260 265 <210> 174 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1XP_003502520.1 <400> 174 Met Pro Arg Ser Phe Leu Val Arg Lys Ala Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ala Cys Val Glu Phe Ile Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ala Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Val Ala Trp Ala Thr Leu Pro Pro Arg 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Ser Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Asp Ser Glu Ala Phe Val Ser 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 175 <211> 349 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004014930.1 <400> 175 Met His Ala Pro Glu Ala Arg Arg Pro Arg Gly Pro Ala Arg Leu Pro 1 5 10 15 Ala Pro Arg Gly Ala Ser Pro Ala Ser Pro Gln Arg Thr Cys Ser Gly 20 25 30 Arg Gly Arg Trp Arg Arg Arg Val Ser Ile Gly Ala Glu Val Thr Lys 35 40 45 Gly Arg Asp Trp Asp Gly Pro Arg Pro Pro Ala Thr Gly Val Leu Lys 50 55 60 Gly Ile Gly Gly Pro Ser Pro Leu Thr Ala Gln Leu His Gly Val Val 65 70 75 80 Gly Gly Glu Leu Cys Asp Pro Gly Ala Thr Gly Glu Thr Pro Pro His 85 90 95 Pro Thr Arg Cys Phe Gly His His Ala Ser Leu His Asp Ser Ser Pro 100 105 110 Glu Leu Thr Phe Gln Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala 115 120 125 Ile Pro Pro Pro Glu Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu 130 135 140 Ile Trp Asp Ser Leu Leu Ala Pro Gln Ala Gln Pro Ile Ser Trp Ala 145 150 155 160 Ser Leu Leu Pro Gln Glu Ile Pro Lys Ala Gly Glu Leu Thr Ser Leu 165 170 175 Ser Asp Glu Asp Ser Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser 180 185 190 Pro Ala Pro Ser Ser Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala 195 200 205 Glu Gly Tyr Ala Ala Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu 210 215 220 Thr Gly Leu Ser Val Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn 225 230 235 240 Cys Lys Tyr Cys Asn Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met 245 250 255 His Ile Arg Ser His Thr Leu Pro Cys Val Cys Ser Thr Cys Gly Lys 260 265 270 Ala Phe Ser Arg Pro Trp Leu Leu Gln Gly His Val Arg Thr His Thr 275 280 285 Gly Glu Lys Pro Phe Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp 290 295 300 Arg Ser Asn Leu Arg Ala His Leu Gln Thr His Ser Asp Val Lys Lys 305 310 315 320 Tyr Gln Cys Lys Thr Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu 325 330 335 His Lys His Gln Glu Ser Gly Cys Ser Gly Gly Pro Arg 340 345 <210> 176 <211> 262 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004663926.1 <400> 176 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Ser Leu Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ala Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro His Val Gln Pro Val Ala Trp Ala Ser Phe Pro Pro Arg 65 70 75 80 Glu Ser Pro Lys Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Ala Ser Ser Leu Glu Ala Glu Ala Phe Ala Val Phe Pro 115 120 125 Thr Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Val Ala Lys 130 135 140 Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn Lys Glu 145 150 155 160 Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His Thr Leu 165 170 175 Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro Trp Leu 180 185 190 Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe Ser Cys 195 200 205 Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg Ala His 210 215 220 Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Gln Ala Cys Ala 225 230 235 240 Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu Ser Gly 245 250 255 Cys Ser Gly Gly Pro Arg 260 <210> 177 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_006758858.1 <400> 177 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Thr Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Val Gly Trp Ala Ser Leu Leu Pro Arg 65 70 75 80 Glu Ile Pro Lys Val Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Asp Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Pro Leu Pro Lys Gln Leu Ser Gly Leu Ser Gly 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Glu Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 178 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_006098041.1 <400> 178 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Val Gly Trp Ala Ser Leu Leu Pro Arg 65 70 75 80 Glu Ile Pro Lys Val Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Asp Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Pro Leu Pro Lys Gln Leu Ser Gly Leu Ser Gly 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Glu Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 179 <211> 266 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_007936620.1 <400> 179 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Leu Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Met Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Ser Gly Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Gly Pro Lys Ala Thr Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Ala Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Val Leu Ala Ala 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asp 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Asp Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Ala Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Glu Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg Gly Pro 260 265 <210> 180 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004462255.1 <400> 180 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Leu Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro His Ala Gln Pro Val Ser Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Gly Pro Lys Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Ala Ser Ala Ser Ser Leu Glu Ala Glu Thr Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Pro Pro Lys Gln Leu Ala Val Leu Ala Val 130 135 140 Ala Arg Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asp 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Asp Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Glu Glu 245 250 255 Ser Gly Cys Ala Gly Gly Pro Arg 260 <210> 181 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_008139391.1 <400> 181 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Leu Gly Trp Pro Ser Leu Leu Pro Arg 65 70 75 80 Glu Ile Pro Lys Val Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Ser Leu Gly Pro Leu Pro Lys Gln Leu Ser Gly Leu Ser Gly 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 182 <211> 274 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004636165.1 <400> 182 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Thr Asp Pro Asn Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Ser Pro Glu Leu Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Val 50 55 60 Leu Val Pro Leu Thr Gln Pro Leu Arg Pro Ala Ser Leu Gln Pro Leu 65 70 75 80 His Pro Ala Ser Leu Gln Pro Gln Glu Ser Pro Ser Pro Lys Ala Ala 85 90 95 Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser Gly Lys Gly Ser Gln Pro 100 105 110 Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser Phe Ser Ser Ala Ser Thr 115 120 125 Ser Ser Leu Glu Ala Glu Ala Cys Ala Ala Phe Pro Gly Leu Gly Gln 130 135 140 Leu Pro Lys Gln Leu Ala Gly Leu Ser Met Ala Lys Asp Leu Gln Cys 145 150 155 160 Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn Lys Glu Tyr Leu Ser Leu 165 170 175 Gly Ala Leu Lys Met His Ile Arg Ser His Thr Leu Pro Cys Val Cys 180 185 190 Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro Trp Leu Leu Gln Gly His 195 200 205 Val Arg Thr His Thr Gly Glu Lys Pro Phe Ser Cys Ser His Cys Ser 210 215 220 Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg Ala His Leu Gln Thr His 225 230 235 240 Ser Asp Val Lys Lys Tyr Arg Cys Lys Thr Cys Ser Arg Thr Phe Ser 245 250 255 Arg Met Ser Leu Leu His Lys His Gln Glu Ser Gly Cys Ser Gly Gly 260 265 270 Pro His <210> 183 <211> 266 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_006921969.1 <400> 183 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Val Gly Arg Ala Ser Leu Leu Ala Gln 65 70 75 80 Glu Ile Pro Lys Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Ser Gly Leu Gly Pro Leu Pro Gly Leu Lys Gln Leu Ala Gly Leu 130 135 140 Ser Gly Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr 145 150 155 160 Cys Asn Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg 165 170 175 Ser His Thr Leu Pro Cys Val Cys Ser Thr Cys Gly Lys Ala Phe Ser 180 185 190 Arg Pro Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys 195 200 205 Pro Phe Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn 210 215 220 Leu Arg Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys 225 230 235 240 Lys Thr Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His 245 250 255 Gln Glu Ser Gly Cys Ser Gly Gly Pro Arg 260 265 <210> 184 <211> 339 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004370603.1 <400> 184 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Leu Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Ser Gly Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Cys Pro Lys Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Ser Ser Ser 100 105 110 Phe Ser Ser Gly Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Ser Gln Val Pro Lys Gln Leu Ala Val Leu Ala Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asp 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Asp Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Ala Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Glu Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg Gly Pro Arg Ala Pro Leu Gln Ala 260 265 270 Ala Gly Pro Leu Pro Ala Gly Pro Gln Arg Asp Glu Cys Ser Thr Arg 275 280 285 Arg Pro Leu Trp Gly Pro Gln Thr Ala Arg Arg Gln Ala Asn Pro Ala 290 295 300 Trp Lys Ile Arg Arg Trp Lys Ala Arg Arg Leu Asn Glu Gly Ala Ala 305 310 315 320 Ser Ser Gly Gly Gly Ser Glu Gly Ala Glu Gly Ala Ala Gly Ala Trp 325 330 335 Pro Glu Thr <210> 185 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004618816.1 <400> 185 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ser Cys Pro Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Cys Pro Glu Leu Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Arg Ala Pro Pro Pro Ser Trp Ala Ala Leu Leu Pro Ser 65 70 75 80 Asp Leu Pro Gln Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Pro Leu Pro Lys Gln Leu Ser Gly Leu Ala Leu 130 135 140 Ala Lys Asp Pro Gln Ala Arg Lys Ala Phe Asn Cys Lys Tyr Cys Thr 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Asp Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Thr His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Pro Ala 225 230 235 240 Cys Ala Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ala Gly Gly Pro Arg 260 <210> 186 <211> 224 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004325726.1 <400> 186 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Ile Pro Lys Ala Ile Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ser Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Tyr Pro Cys 195 200 205 Arg Leu Ala Ala Leu Leu Thr Val Pro Leu Leu Leu Leu Gln Ala Leu 210 215 220 <210> 187 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_007533425.1 <400> 187 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ser Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Leu Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Thr His Leu Leu Ala Thr Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Ser Ile Thr Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Leu Pro Lys Ala Val Asp Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Thr 100 105 110 Phe Ser Ser Ala Ser Phe Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser Met 130 135 140 Ala Lys Asp Leu Gln Ala Arg Lys Ser Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Ile Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Asp Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Ile Lys Lys Tyr Gln Cys Lys Met 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Leu Gly Gly Pro His 260 <210> 188 <211> 266 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_003419974.1 <400> 188 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Leu Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Ser Gly Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Gly Pro Lys Ala Thr Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Ser Ser Ser 100 105 110 Phe Ser Ser Gly Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Pro 115 120 125 Phe Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Val Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Ser Cys Lys Tyr Cys Asp 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Asp Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Ala Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Glu Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg Gly Pro 260 265 <210> 189 <211> 267 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_005574198.1 <400> 189 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asp Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Pro Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr His Arg Ala His Leu Leu Ala Ala Met Pro Arg Ser Glu 35 40 45 Ile Leu Asn Pro Ser Ala Ser Pro Pro Thr Leu Ile Trp Asp Ala Phe 50 55 60 Leu Ala Leu Gln Ala Gln Pro Thr Ala Arg Ala Ser Leu Gln Leu Gly 65 70 75 80 Leu Ser Gln Glu Ser Pro Lys Val Ala Glu Leu Thr Ser Leu Ser Asp 85 90 95 Glu Asp Ser Gly Lys Gly Ser Gln Ser Pro Ser Pro Pro Ser Gln Ala 100 105 110 Pro Ser Ser Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala 115 120 125 Asp Ala Thr Ser Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln 130 135 140 Val Ser Glu Ala Lys Asp Ser Gln Phe Gln Lys Ala Phe Asn Cys Gln 145 150 155 160 Tyr Cys Asn Lys Glu Cys Leu Ser Leu Gly Val Leu Lys Arg His Ile 165 170 175 Arg Ser His Thr Leu Pro Phe Val Cys Gly Thr Cys Gly Lys Ala Phe 180 185 190 Ser Arg Pro Trp Leu Leu Arg Gly His Val Arg Thr His Thr Gly Glu 195 200 205 Lys Pro Phe Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser 210 215 220 Asn Leu Arg Ala His Leu Gln Thr His Leu Asp Ile Lys Lys Tyr Gln 225 230 235 240 Cys Gln Ala Cys Thr Arg Thr Phe Ser Arg Met Ala Leu Leu His Lys 245 250 255 His Gln Glu Ser Gly Cys Ser Gly Gly Pro Cys 260 265 <210> 190 <211> 206 <212> PRT <213> Artificial Sequence <220> <223> Snail1 AAQ21376.1 <400> 190 Met Pro Arg Ser Val Leu Val Lys Lys Pro Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Ile Pro Arg Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Pro Glu Ala Glu Ser Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Phe Arg Pro 180 185 190 Trp Leu Val Glu Gly His Val Arg Thr His Thr Gly Glu Lys 195 200 205 <210> 191 <211> 263 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_007641286.1 <400> 191 Met Pro Arg Ser Phe Leu Val Arg Lys Ala Ser Asp Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ala Cys Val Glu Phe Ile Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Ala Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Val Ala Trp Ala Thr Leu Pro Pro Arg 65 70 75 80 Glu Ser Pro Arg Ala Ala Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Ser Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Asp Ser Glu Ala Phe Val Ser 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Arg Leu Ser Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Leu Gln Lys Gly 195 200 205 Gln Met Ala Asn Asn Gln Lys Cys Ser Val Pro Ala Arg Arg Thr Gly 210 215 220 Leu Thr Val Asp Arg Thr Leu Gly Gly Ser Leu Glu Thr Gln Lys Ser 225 230 235 240 Asp Gly Gln Gly Tyr Thr Gln Glu Ile Gln Arg Pro Glu Thr Pro His 245 250 255 Pro Val Gln Ala Cys Ala Gln 260 <210> 192 <211> 264 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_005866258.1 <400> 192 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Leu Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Thr Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Val Gly Trp Ala Ser Leu Leu Pro Arg 65 70 75 80 Glu Ile Pro Lys Val Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Asp Gly Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Pro Leu Pro Lys Gln Leu Ser Gly Leu Ser Gly 130 135 140 Ala Lys Asp Pro Arg Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Val Arg Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Glu Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg 260 <210> 193 <211> 266 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_006839389.1 <400> 193 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Leu Glu Phe Thr Phe Glu 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Lys Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Ser Ile Trp Ala Ser Leu Gln Thr Gln 65 70 75 80 Glu Gly Pro Lys Ala Ser Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Ala Ser Ala Ser Ser Leu Glu Ala Glu Ala Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Pro Val Pro Lys Gln Leu Ala Val Leu Ala Gly 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asp 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Asp Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Ala Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Glu Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg Gly Pro 260 265 <210> 194 <211> 266 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004698375.1 <400> 194 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Leu Glu Phe Ala Ile Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Arg Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Gln Val Gln Pro Ser Gly Trp Ala Ser Leu Pro Pro Gln 65 70 75 80 Glu Gly Pro Lys Ala Ser Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Ala Ser Ala Ser Ser Leu Glu Ala Glu Ala Phe Ala Ala 115 120 125 Phe Thr Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Val Leu Thr Val 130 135 140 Ala Lys Asp Pro Gln Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asp 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Asp Ile Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Gln Thr His Ser Ala Val Lys Lys Tyr Gln Cys Arg Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Glu Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Arg Gly Pro 260 265 <210> 195 <211> 267 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_006881815.1 <400> 195 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Arg Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Leu Pro Gln Leu Gln Pro Ser Ala Trp Ala Ser Leu Arg Pro Gln 65 70 75 80 Glu Gly Pro Lys Ala Ala Glu Leu Thr Phe Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Ala Ser Ala Ser Ser Leu Glu Ala Glu Ala Phe Thr Ala 115 120 125 Phe Pro Gly Leu Gly Pro Val Pro Lys Pro Pro Leu Ala Val Leu Ala 130 135 140 Gly Thr Lys Asp Pro Gln Ala Arg Lys Ala Phe Asn Cys Lys Tyr Cys 145 150 155 160 Asp Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser 165 170 175 His Thr Leu Pro Cys Val Cys Asp Thr Cys Gly Lys Ala Phe Ser Arg 180 185 190 Pro Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro 195 200 205 Phe Ser Cys Ser His Cys Asn Arg Ala Phe Ala Asp Arg Ser Asn Leu 210 215 220 Arg Ala His Leu Gln Thr His Ser Ala Val Lys Lys Tyr Gln Cys Lys 225 230 235 240 Ser Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Glu 245 250 255 Glu Ser Gly Cys Ser Gly Gly Pro Arg Gly Pro 260 265 <210> 196 <211> 267 <212> PRT <213> Artificial Sequence <220> <223> Snail1 EHH21632.1 <400> 196 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ser Asp Pro Asp Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Pro Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr His Arg Ala His Leu Leu Ala Ala Met Pro Arg Ser Glu 35 40 45 Ile Leu Asn Pro Ser Ala Ser Pro Pro Thr Leu Ile Trp Asp Ala Phe 50 55 60 Leu Ala Leu Gln Ala Gln Pro Thr Ala Arg Ala Ser Leu Gln Leu Gly 65 70 75 80 Leu Ser Gln Glu Ser Ser Lys Val Ala Glu Leu Thr Ser Leu Ser Asp 85 90 95 Glu Asp Ser Gly Lys Gly Ser Gln Ser Pro Ser Pro Pro Ser Gln Ala 100 105 110 Pro Ser Ser Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ala 115 120 125 Asp Ala Thr Ser Pro Gly Leu Gly Gln Val Pro Lys Gln Leu Ala Gln 130 135 140 Val Ser Glu Ala Lys Asp Ser Gln Phe Gln Lys Ala Phe Asn Cys Gln 145 150 155 160 Tyr Cys Asn Lys Glu Cys Leu Ser Leu Gly Val Leu Lys Arg His Ile 165 170 175 Arg Ser His Thr Leu Pro Phe Val Cys Gly Thr Cys Gly Lys Ala Phe 180 185 190 Ser Arg Pro Trp Leu Leu Arg Gly His Val Arg Thr His Thr Gly Glu 195 200 205 Lys Pro Phe Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg Ser 210 215 220 Asn Leu Arg Ala His Leu Gln Thr His Leu Asp Ile Lys Lys Tyr Gln 225 230 235 240 Cys Gln Ala Cys Thr Arg Thr Phe Ser Arg Met Ala Leu Leu His Lys 245 250 255 His Gln Glu Ser Gly Cys Ser Gly Gly Pro Cys 260 265 <210> 197 <211> 263 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_005860983.1 <400> 197 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Leu Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Arg 65 70 75 80 Glu Ile Pro Lys Val Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Leu Ser Ser 100 105 110 Ser Ser Thr Leu Ala Ser Ser Leu Glu Ala Asp Gly Tyr Ala Ala Phe 115 120 125 Pro Gly Leu Gly Pro Leu Pro Lys Gln Arg Ser Gly Leu Ser Gly Ala 130 135 140 Lys Asp Pro Arg Ser Arg Lys Ala Phe Asn Cys Lys Tyr Cys Asn Lys 145 150 155 160 Glu Tyr Val Arg Leu Gly Ala Leu Lys Met His Ile Arg Ser His Thr 165 170 175 Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro Trp 180 185 190 Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe Ser 195 200 205 Cys Ser His Cys Asn His Ala Phe Ala Asn Arg Ser Asn Leu Arg Ala 210 215 220 His Leu Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr Cys 225 230 235 240 Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu Ser 245 250 255 Gly Cys Ser Gly Gly Pro Arg 260 <210> 198 <211> 249 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_005658936.1 <400> 198 Met Pro Arg Ser Phe Leu Val Arg Lys Pro Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Ala Ile Pro Pro Pro Glu 35 40 45 Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Ala Pro Gln Ala Gln Pro Ile Gly Trp Ala Ser Leu Leu Pro Gln 65 70 75 80 Glu Ile Pro Arg Ala Val Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser 85 90 95 Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser 100 105 110 Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu Ser Tyr Ala Ala 115 120 125 Phe Pro Gly Leu Gly Gln Leu Pro Lys Gln Leu Ala Gly Leu Ser Xaa 130 135 140 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Lys Glu Tyr Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Cys Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Pro 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys 210 215 220 Thr Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln 225 230 235 240 Glu Ser Gly Cys Ser Gly Gly Pro Arg 245 <210> 199 <211> 292 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_006099783.1 <400> 199 Met Pro Arg Cys Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Arg Pro Asn Tyr Ser Glu Leu His Asp Ser Ser Pro Glu Phe Thr Phe 20 25 30 Gln Gln Pro Tyr Asp Pro Ala His Leu Leu Ala Ala Ile Pro Pro Pro 35 40 45 Glu Val Leu Asn Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser 50 55 60 Leu Leu Ala Pro Gln Ala Gln Pro Val Gly Trp Ala Ser Leu Leu Pro 65 70 75 80 Arg Glu Ile Pro Glu Val Val Glu Leu Thr Ser Leu Ser Asp Glu Asp 85 90 95 Ser Gly Lys Gly Ser Gln Pro Pro Ser Pro Pro Ser Pro Val Pro Cys 100 105 110 Ser Ser Ser Thr Ser Ala Ser Phe Leu Glu Ala Glu Gly His Ala Ala 115 120 125 Phe Leu Gly Leu Gly Pro Leu Pro Lys Gln Leu Ser Gly Leu Ser Gly 130 135 140 Ala Lys Gly Pro Gln Ser His Lys Ala Phe Asn Cys Lys Tyr Cys Asn 145 150 155 160 Thr Glu Tyr Gly Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His 165 170 175 Thr Leu Pro Trp Val Cys Gly Thr Cys Gly Lys Ala Phe Ser Arg Ser 180 185 190 Trp Leu Leu Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe 195 200 205 Ser Cys Ser His Cys Asn Tyr Ala Phe Ala Asp Arg Ser Asn Leu Arg 210 215 220 Ala His Leu Arg Thr His Ser Asp Val Lys Lys Tyr Gln Cys Lys Thr 225 230 235 240 Cys Ser Arg Thr Phe Ser Arg Leu Ser Arg Leu His Lys His Gln Glu 245 250 255 Ser Gly Cys Ser Gly Gly Pro Pro Ala Asp Pro Gln Gly Cys Phe Cys 260 265 270 Leu Ser Arg Pro Ser Pro Cys Ser Ser Leu Pro Gln Leu Trp Lys Glu 275 280 285 Gly Pro Leu Glu 290 <210> 200 <211> 258 <212> PRT <213> Artificial Sequence <220> <223> Snail1 EAW70471.1 <400> 200 Met Pro Arg Ser Phe Leu Val Arg Lys Leu Pro Asp Pro Asp Arg Lys 1 5 10 15 Pro Asn Tyr Ser Lys Leu Pro Asp Ser Asn Pro Glu Phe Thr Phe Gln 20 25 30 Gln Gln Pro Tyr His Gln Ala His Leu Asn Ser Thr Ala Leu Leu Pro 35 40 45 Thr Leu Ile Trp Asp Ala Phe Leu Ala Leu Gln Ala Gln Pro Ala Ala 50 55 60 Arg Ala Ser Leu Gln Leu Arg Leu Pro Gln Glu Ser Phe Lys Ala Ala 65 70 75 80 Glu Leu Thr Ser Leu Ser Asp Glu Asp Ser Gly Lys Gly Ser Gln Ser 85 90 95 Pro Ser Pro Arg Ser Gln Pro Ser Ser Ser Phe Ser Ser Thr Ser Ala 100 105 110 Ser Ser Leu Glu Ala Glu Ala Asp Thr Thr Phe Pro Gly Leu Gly Gln 115 120 125 Val Cys Lys Gln Leu Ala Gln Val Ser Glu Ala Lys Asp Ser Gln Phe 130 135 140 Gln Lys Ala Phe Asn Cys Gln Tyr Cys Asn Lys Glu Cys Leu Ser Leu 145 150 155 160 Gly Ala Leu Lys Lys His Ile Arg Ser His Thr Leu Pro Phe Ala Cys 165 170 175 Glu Thr Cys Gly Lys Ala Phe Ser Arg Pro Trp Leu Leu Arg Gly His 180 185 190 Val Arg Thr His Thr Gly Glu Lys Pro Phe Ser Cys Thr His Cys Ser 195 200 205 Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg Ala His Leu Gln Thr His 210 215 220 Leu Asp Val Lys Lys Tyr Gln Cys Gln Ala Cys Ala Arg Thr Phe Ser 225 230 235 240 Arg Met Ala Leu Leu His Lys His Gln Glu Ser Gly Cys Ser Gly Gly 245 250 255 Pro Arg <210> 201 <211> 261 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004585952.1 <400> 201 Met Pro Arg Ser Phe Leu Val Arg Lys Gln Ser Val Pro Arg Arg Lys 1 5 10 15 Pro Asn Tyr Ser Glu Leu Gln Asp Ser Ser Leu Glu Phe Thr Phe Gln 20 25 30 Gln Pro Tyr Asp Gln Ala His Leu Leu Ala Thr Ile Pro Pro Pro Glu 35 40 45 Val Leu Lys Pro Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Leu 50 55 60 Leu Val Pro Pro Val Pro Arg Gly Pro Val Val Ala Trp Ala Ala Leu 65 70 75 80 Gln Val Ala Glu Leu Thr Val Leu Ser Asp Glu Asp Ser Gly Gln Gly 85 90 95 Ser Gln Pro Pro Ser Pro Pro Ser Pro Ala Pro Ser Ser Phe Ser Ser 100 105 110 Ala Ser Leu Ser Ser Leu Glu Ala Glu Val Cys Thr Thr Cys Pro Ala 115 120 125 Leu Gly Pro Leu Pro Arg Pro Pro Ser Gln Leu Cys Glu Thr Lys Glu 130 135 140 Pro Gln Ala Arg Lys Ala Phe Thr Cys Lys Tyr Cys Asp Lys Glu Tyr 145 150 155 160 Leu Ser Leu Gly Ala Leu Lys Met His Ile Arg Ser His Thr Leu Pro 165 170 175 Cys Val Cys Ala Thr Cys Gly Lys Ala Phe Ser Arg Pro Trp Leu Leu 180 185 190 Gln Gly His Val Arg Thr His Thr Gly Glu Lys Pro Phe Ser Cys Ser 195 200 205 His Cys Ser Arg Ala Phe Ala Asp Arg Ser Asn Leu Arg Ala His Leu 210 215 220 Gln Thr His Ser Asp Val Lys Lys Tyr Gln Cys Val Val Cys Ala Arg 225 230 235 240 Thr Phe Ser Arg Met Ser Leu Leu His Lys His Gln Glu Ser Gly Cys 245 250 255 Met Ala Gly Pro Arg 260 <210> 202 <211> 3573 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004033198.1 <400> 202 Met Thr His Lys Pro Cys Ser Ile Leu Ser Phe Tyr Tyr Asp Cys Thr 1 5 10 15 Arg Asn Ala Phe Leu Arg Pro Lys Leu Gly Lys Gln Tyr Glu Ala Ser 20 25 30 Cys Val Ser Phe Glu Arg Val Leu Val Glu Asn Lys Leu His Gly Leu 35 40 45 Ser Pro Ala Leu Ser Glu Ala Ile Gln Ser Ile Ser Arg Trp Glu Leu 50 55 60 Val Gln Ala Ala Leu Pro His Val Leu His Cys Thr Ala Thr Leu Leu 65 70 75 80 Ser Asn Arg Asn Lys Leu Gly His Gln Asp Lys Leu Gly Val Ala Glu 85 90 95 Thr Lys Leu Leu His Thr Leu His Trp Met Leu Leu Glu Ala Pro Gln 100 105 110 Asp Cys Asn Asn Glu Arg Phe Gly Gly Thr Asp Arg Gly Ser Ser Trp 115 120 125 Gly Gly Ser Ser Ser Ala Phe Ile His Gln Val Glu Asn Gln Gly Ser 130 135 140 Pro Gly Gln Pro Cys Gln Ser Ser Ser Asn Asp Glu Glu Glu Asn Asn 145 150 155 160 Arg Arg Lys Ile Phe Gln Asn Ser Met Ala Thr Val Glu Leu Phe Val 165 170 175 Phe Leu Phe Ala Pro Leu Val His Arg Ile Lys Glu Ser Asp Leu Thr 180 185 190 Phe Arg Leu Ala Ser Gly Leu Val Ile Trp Gln Pro Met Trp Glu His 195 200 205 Arg Gln Pro Glu Val Ser Gly Phe Thr Ala Leu Val Lys Pro Ile Arg 210 215 220 Asn Ile Ile Thr Ala Lys Arg Ser Ser Pro Ile Asn Ser Gln Ser Arg 225 230 235 240 Thr Cys Glu Ser Leu Asn Gln Asp Ala Arg His Leu Glu Arg Ala Gly 245 250 255 Val Glu Ile Arg Pro Tyr Thr Thr Ser Ile Leu Ser Arg Trp Leu Val 260 265 270 Ser Thr Ser Leu Pro Leu Arg Tyr Ser Ala Thr Ser Leu Pro Leu Arg 275 280 285 Tyr Ser Ala Thr Ser Leu Pro Leu Arg Tyr Ser Ala Thr Ser Leu Pro 290 295 300 Leu Arg Tyr Ser Ala Thr Ser Leu Pro Leu Arg Tyr Ser Ala Thr Ser 305 310 315 320 Leu Pro Leu Arg Tyr Ser Ala Thr Ser Leu Pro Leu Arg Tyr Ser Ala 325 330 335 Thr Ser Leu Pro Leu Arg Tyr Ser Ala Thr Ser Leu Pro Leu Arg Tyr 340 345 350 Ser Ala Thr Ser Leu Pro Leu Arg Tyr Ser Ala Thr Ser Xaa Xaa Xaa 355 360 365 Xaa Xaa Leu Ser Asp Leu Val Ala Ser Thr Thr Met Pro Arg Ser Phe 370 375 380 Leu Val Arg Lys Leu Pro Asp Pro Asp Arg Lys Pro Asn Tyr Ser Lys 385 390 395 400 Leu Pro Asp Ser Asn Pro Glu Phe Thr Phe Gln Gln Pro Tyr His Gln 405 410 415 Asp His Leu Asn Ser Thr Ala Leu Leu Pro Thr Leu Ile Trp Asp Ala 420 425 430 Phe Leu Ala Leu Gln Ala Gln Pro Thr Ala Arg Ala Ser Leu Gln Leu 435 440 445 Arg Leu Pro Gln Glu Ser Ser Lys Ala Ala Glu Leu Thr Ser Leu Ser 450 455 460 Asp Glu Asp Ser Gly Lys Gly Ser Gln Ser Pro Ser Pro Arg Ser Gln 465 470 475 480 Pro Ser Ser Ser Phe Ser Ser Thr Ser Ala Ser Ser Leu Glu Ala Glu 485 490 495 Ala Asp Thr Thr Phe Pro Gly Leu Gly Gln Val Cys Lys Gln Leu Ala 500 505 510 Gln Val Ser Glu Ala Lys Asp Ser Gln Phe Gln Lys Ala Phe Asn Cys 515 520 525 Gln Tyr Cys Asn Lys Glu Cys Leu Ser Leu Gly Ala Leu Lys Lys His 530 535 540 Ile Arg Ser His Thr Leu Pro Phe Ala Cys Glu Thr Cys Gly Lys Ala 545 550 555 560 Phe Ser Arg Pro Trp Leu Leu Arg Gly His Val Arg Thr His Thr Gly 565 570 575 Glu Lys Pro Phe Ser Cys Pro His Cys Ser Arg Ala Phe Ala Asp Arg 580 585 590 Ser Asn Leu Arg Ala His Leu Gln Thr His Leu Asp Val Lys Lys Tyr 595 600 605 Gln Cys Gln Ala Cys Ala Arg Thr Phe Ser Arg Met Ala Leu Leu His 610 615 620 Lys His Gln Glu Ala Ser Leu Val Ile Pro Pro Cys Gln Arg Ser Arg 625 630 635 640 Tyr Ala Thr Tyr Phe Asp Val Ala Val Leu Arg Cys Leu Leu Gln Pro 645 650 655 His Trp Ser Glu Glu Gly Thr Gln Trp Ser Leu Met Tyr Tyr Leu Gln 660 665 670 Arg Leu Arg His Met Leu Glu Glu Lys Pro Glu Lys Pro Pro Glu Pro 675 680 685 Asp Ile Pro Leu Leu Pro Arg Pro Arg Ser Ser Ser Met Val Ala Ala 690 695 700 Ala Pro Ser Leu Val Asn Thr His Lys Thr Gln Asp Leu Thr Met Lys 705 710 715 720 Cys Asn Glu Glu Glu Lys Ser Leu Ser Ser Glu Ala Phe Ser Lys Val 725 730 735 Ser Leu Thr Asn Leu Arg Arg Ser Ala Val Pro Asp Leu Ser Ser Asp 740 745 750 Leu Gly Met Asn Ile Phe Lys Lys Phe Lys Ser Arg Lys Glu Asp Arg 755 760 765 Glu Arg Lys Gly Ser Ile Pro Phe His His Thr Gly Lys Arg Arg Pro 770 775 780 Arg Arg Met Gly Val Pro Phe Leu Leu His Glu Asp His Leu Asp Val 785 790 795 800 Ser Pro Thr Arg Ser Thr Phe Ser Phe Gly Ser Phe Ser Gly Leu Gly 805 810 815 Glu Asp Arg Arg Gly Ile Glu Lys Gly Gly Trp Gln Thr Thr Ile Leu 820 825 830 Gly Lys Leu Thr Arg Arg Gly Ser Ser Asp Ala Ala Thr Glu Met Glu 835 840 845 Ser Leu Ser Ala Arg His Ser His Ser His His Thr Leu Val Ser Asp 850 855 860 Leu Pro Asp Pro Ser Asn Ser His Gly Glu Asn Thr Val Lys Glu Val 865 870 875 880 Arg Ser Gln Ile Ser Thr Ile Thr Val Ala Thr Phe Asn Thr Thr Leu 885 890 895 Ala Ser Phe Asn Val Gly Tyr Ala Asp Phe Phe Asn Glu His Met Arg 900 905 910 Lys Leu Cys Asn Gln Val Pro Ile Pro Glu Met Pro His Glu Pro Leu 915 920 925 Ala Cys Ala Asn Leu Pro Arg Ser Leu Thr Asp Ser Cys Ile Asn Tyr 930 935 940 Ser Tyr Leu Glu Asp Thr Glu His Ile Asp Gly Thr Asn Asn Phe Val 945 950 955 960 His Lys Asn Gly Met Leu Asp Leu Ser Val Val Leu Lys Ala Val Tyr 965 970 975 Leu Val Leu Asn His Asp Ile Ser Ser Arg Ile Cys Asp Val Ala Leu 980 985 990 Asn Ile Val Glu Cys Leu Leu Gln Leu Gly Val Val Pro Cys Val Glu 995 1000 1005 Lys Asn Arg Lys Lys Ser Glu Asn Lys Glu Asn Glu Thr Leu Glu Lys 1010 1015 1020 Arg Pro Ser Glu Gly Ala Phe Gln Phe Lys Gly Val Ser Gly Ser Ser 1025 1030 1035 1040 Thr Cys Gly Phe Gly Gly Pro Ala Val Ser Gly Ala Gly Asp Gly Gly 1045 1050 1055 Gly Glu Glu Gly Gly Gly Gly Asp Gly Gly Gly Gly Gly Gly Asp Gly 1060 1065 1070 Gly Gly Gly Gly Gly Gly Gly Gly Gly Pro Tyr Glu Lys Asn Asp Lys 1075 1080 1085 Asn Gln Glu Lys Asp Glu Ser Thr Pro Val Ser Asn His Arg Leu Ala 1090 1095 1100 Leu Thr Met Leu Ile Lys Ile Val Lys Ser Leu Gly Cys Ala Tyr Gly 1105 1110 1115 1120 Cys Gly Glu Gly His Arg Gly Leu Ser Gly Asp Arg Leu Arg His Gln 1125 1130 1135 Val Phe Arg Glu Asn Ala Gln Asn Cys Leu Thr Lys Leu Tyr Lys Leu 1140 1145 1150 Asp Lys Met Gln Phe Arg Gln Thr Met Arg Asp Tyr Val Asn Lys Asp 1155 1160 1165 Ser Leu Asn Asn Val Val Asp Phe Leu His Ala Leu Leu Gly Phe Cys 1170 1175 1180 Met Glu Pro Val Thr Asp Asn Lys Ala Gly Phe Gly Asn Asn Phe Thr 1185 1190 1195 1200 Thr Val Asp Asn Lys Ser Thr Ala Gln Asn Val Glu Gly Ile Ile Val 1205 1210 1215 Ser Ala Met Phe Lys Ser Leu Ile Thr Arg Cys Ala Ser Thr Thr His 1220 1225 1230 Glu Leu His Ser Pro Glu Asn Leu Gly Leu Tyr Cys Asp Ile Arg Gln 1235 1240 1245 Leu Val Gln Phe Ile Lys Glu Ala His Gly Asn Val Phe Arg Arg Val 1250 1255 1260 Ala Leu Ser Ala Leu Leu Asp Ser Ala Glu Lys Leu Ala Pro Gly Lys 1265 1270 1275 1280 Lys Val Glu Glu Asn Glu Gln Glu Ser Lys Pro Ala Val Ser Lys Arg 1285 1290 1295 Ser Glu Ala Gly Ser Ile Val Asp Lys Gly Gln Val Ser Ser Ala Pro 1300 1305 1310 Glu Glu Cys Arg Ser Phe Met Ser Gly Arg Pro Ser Gln Thr Pro Glu 1315 1320 1325 His Asp Glu Gln Met Gln Gly Ala Asn Leu Gly Arg Lys Asp Phe Trp 1330 1335 1340 Arg Lys Met Phe Lys Ser Gln Ser Ala Ala Ser Asp Thr Ser Ser Gln 1345 1350 1355 1360 Ser Glu Gln Asp Thr Ser Glu Cys Thr Thr Ala His Ser Gly Thr Thr 1365 1370 1375 Ser Asp Arg Arg Ser Arg Ser Arg Ser Arg Arg Ile Ser Leu Arg Lys 1380 1385 1390 Lys Leu Lys Leu Pro Ile Gly Lys Arg Asn Trp Leu Lys Arg Ser Ser 1395 1400 1405 Leu Ser Gly Leu Ala Asp Gly Val Glu Asp Leu Leu Asp Ile Ser Ser 1410 1415 1420 Val Asp Arg Leu Ser Phe Ile Arg Gln Ser Ser Lys Val Lys Phe Thr 1425 1430 1435 1440 Ser Ala Val Lys Leu Ser Glu Gly Gly Pro Gly Ser Gly Met Glu Asn 1445 1450 1455 Gly Arg Asp Glu Glu Glu Asn Phe Phe Lys Arg Leu Gly Cys His Ser 1460 1465 1470 Phe Asp Asp His Leu Ser Pro Asn Gln Asp Gly Gly Lys Ser Lys Asn 1475 1480 1485 Val Val Asn Leu Gly Ala Ile Arg Gln Gly Met Lys Arg Phe Gln Phe 1490 1495 1500 Leu Leu Asn Cys Cys Glu Pro Gly Thr Ile Pro Asp Ala Ser Ile Leu 1505 1510 1515 1520 Ala Ala Ala Leu Asp Leu Glu Ala Pro Val Val Ala Arg Ala Ala Leu 1525 1530 1535 Phe Leu Glu Cys Ala Arg Phe Val His Arg Cys Asn Arg Gly Asn Trp 1540 1545 1550 Pro Glu Trp Met Lys Gly His His Val Asn Ile Thr Lys Lys Gly Leu 1555 1560 1565 Ser Arg Gly Arg Ser Pro Val Val Gly Asn Lys Arg Asn Gln Lys Leu 1570 1575 1580 Gln Trp Asn Ala Ala Lys Leu Phe Tyr Gln Trp Gly Asp Ala Ile Gly 1585 1590 1595 1600 Val Arg Leu Asn Glu Leu Cys His Gly Glu Ser Glu Ser Pro Ala Asn 1605 1610 1615 Leu Leu Gly Leu Ile Tyr Asp Glu Glu Thr Lys Arg Arg Leu Arg Lys 1620 1625 1630 Glu Asp Glu Glu Glu Asp Phe Leu Asp Asp Ser Thr Val Asn Pro Ser 1635 1640 1645 Lys Cys Gly Cys Pro Phe Ala Leu Lys Met Ala Ala Cys Gln Leu Leu 1650 1655 1660 Leu Glu Ile Thr Thr Phe Leu Arg Glu Thr Phe Ser Cys Leu Pro Arg 1665 1670 1675 1680 Pro Arg Thr Glu Pro Leu Val Asp Leu Glu Ser Cys Arg Leu Arg Leu 1685 1690 1695 Asp Pro Glu Leu Asp Arg His Arg Tyr Glu Arg Lys Ile Ser Phe Ala 1700 1705 1710 Gly Val Leu Asp Glu Asn Glu Asp Ser Lys Asp Ser Leu His Ser Ser 1715 1720 1725 Ser His Thr Leu Lys Ser Asp Ala Gly Val Glu Glu Lys Lys Glu Gly 1730 1735 1740 Ser Pro Trp Ser Ala Ser Glu Pro Ser Ile Glu Pro Glu Gly Met Ser 1745 1750 1755 1760 Asn Ala Gly Ala Glu Glu Asn Tyr His Arg Asn Met Ser Trp Leu His 1765 1770 1775 Val Met Ile Leu Leu Cys Asn Gln Gln Ser Phe Ile Cys Thr His Val 1780 1785 1790 Asp Tyr Cys His Pro His Cys Tyr Leu His His Ser Arg Ser Cys Ala 1795 1800 1805 Arg Leu Val Arg Ala Ile Lys Leu Leu Tyr Gly Asp Ser Val Asp Ser 1810 1815 1820 Leu Arg Glu Ser Ser Asn Ile Ser Ser Val Ala Leu Arg Gly Lys Lys 1825 1830 1835 1840 Gln Lys Glu Cys Ser Asp Lys Ser Cys Leu Arg Thr Pro Ser Leu Lys 1845 1850 1855 Lys Arg Val Ser Asp Ala Asn Leu Glu Gly Lys Lys Asp Ser Gly Met 1860 1865 1870 Leu Lys Tyr Ile Arg Leu Gln Val Met Ser Leu Ser Pro Ala Pro Leu 1875 1880 1885 Ser Leu Leu Ile Lys Ala Ala Pro Ile Leu Thr Glu Glu Met Tyr Gly 1890 1895 1900 Asp Ile Gln Pro Ala Ala Trp Glu Leu Leu Leu Ser Met Asp Glu His 1905 1910 1915 1920 Met Ala Gly Ala Ala Ala Ala Met Phe Leu Leu Cys Ala Val Lys Val 1925 1930 1935 Pro Glu Ala Val Ser Asp Met Leu Met Ser Glu Phe His His Pro Glu 1940 1945 1950 Thr Val Gln Arg Leu Asn Ala Val Leu Lys Phe His Thr Leu Trp Arg 1955 1960 1965 Phe Arg Tyr Gln Val Trp Pro Arg Met Glu Glu Gly Ala Gln Gln Ile 1970 1975 1980 Phe Lys Ile Pro Pro Pro Ser Ile Asn Phe Thr Leu Pro Ser Pro Val 1985 1990 1995 2000 Leu Gly Met Pro Ser Val Pro Met Phe Asp Pro Pro Trp Val Pro Gln 2005 2010 2015 Cys Ser Gly Ser Val Gln Asp Pro Ile Asn Glu Asp Gln Ser Lys Ser 2020 2025 2030 Phe Ser Ala Arg Ala Val Ser Arg Ser His Gln Arg Ala Glu His Ile 2035 2040 2045 Leu Lys Asn Leu Gln Gln Glu Glu Glu Lys Lys Arg Leu Gly Arg Glu 2050 2055 2060 Ala Ser Leu Ile Thr Ala Ile Pro Ile Thr Gln Glu Ala Cys Tyr Glu 2065 2070 2075 2080 Pro Thr Cys Thr Pro Asn Ser Glu Pro Glu Glu Glu Val Glu Glu Val 2085 2090 2095 Thr Asn Leu Ala Ser Arg Arg Leu Ser Val Ser Pro Ser Cys Thr Ser 2100 2105 2110 Ser Thr Ser His Arg Asn Tyr Ser Phe Arg Arg Gly Ser Val Trp Ser 2115 2120 2125 Val Arg Ser Ala Val Ser Ala Glu Asp Glu Glu His Thr Thr Glu His 2130 2135 2140 Thr Pro Asn His His Val Pro Gln Pro Pro Gln Ala Val Phe Pro Ala 2145 2150 2155 2160 Cys Ile Cys Ala Ala Val Leu Pro Ile Val His Leu Met Glu Asp Gly 2165 2170 2175 Glu Val Arg Glu Asp Gly Val Ala Val Ser Ala Val Ala Gln Gln Val 2180 2185 2190 Leu Trp Asn Cys Leu Ile Glu Asp Pro Ser Thr Val Leu Arg His Phe 2195 2200 2205 Leu Glu Lys Leu Thr Ile Ser Asn Arg Gln Asp Glu Leu Met Tyr Met 2210 2215 2220 Leu Arg Lys Leu Leu Leu Asn Ile Gly Asp Phe Pro Ala Gln Thr Ser 2225 2230 2235 2240 His Ile Leu Phe Asn Tyr Leu Val Gly Leu Ile Met Tyr Phe Val Arg 2245 2250 2255 Thr Pro Cys Glu Trp Gly Met Asp Ala Ile Ser Ala Thr Leu Thr Phe 2260 2265 2270 Leu Trp Glu Val Val Gly Tyr Val Glu Gly Leu Phe Phe Lys Asp Leu 2275 2280 2285 Lys Gln Thr Met Lys Lys Glu Gln Cys Glu Val Lys Leu Leu Val Thr 2290 2295 2300 Ala Ser Met Pro Gly Thr Lys Thr Leu Val Val His Gly Gln Asn Glu 2305 2310 2315 2320 Cys Asp Ile Pro Thr Gln Leu Pro Val His Glu Asp Thr Gln Phe Glu 2325 2330 2335 Ala Leu Leu Lys Glu Cys Leu Glu Phe Phe Asn Ile Pro Glu Ser Gln 2340 2345 2350 Ser Thr His Tyr Phe Leu Met Asp Lys Arg Trp Asn Leu Ile His Tyr 2355 2360 2365 Asn Lys Thr Tyr Val Arg Asp Ile Tyr Pro Phe Arg Arg Ser Val Ser 2370 2375 2380 Pro Gln Leu Asn Leu Val His Met His Pro Glu Lys Gly Gln Glu Leu 2385 2390 2395 2400 Ile Gln Lys Gln Val Phe Thr Arg Lys Leu Glu Glu Val Gly Arg Val 2405 2410 2415 Leu Phe Leu Ile Ser Leu Thr Gln Lys Ile Pro Thr Ala His Lys Gln 2420 2425 2430 Ser His Val Ser Met Leu Gln Glu Asp Leu Leu Arg Leu Pro Ser Phe 2435 2440 2445 Pro Arg Ser Ala Ile Asp Ala Glu Phe Ser Leu Phe Ser Asp Pro Gln 2450 2455 2460 Ala Gly Lys Glu Leu Phe Gly Leu Asp Thr Leu Gln Lys Ser Leu Trp 2465 2470 2475 2480 Ile Gln Leu Leu Glu Glu Met Phe Leu Gly Met Pro Ser Glu Phe Pro 2485 2490 2495 Trp Gly Asp Glu Ile Met Leu Phe Leu Asn Val Phe Asn Gly Ala Leu 2500 2505 2510 Ile Leu His Pro Glu Asp Ser Ala Leu Leu Arg Gln Tyr Ala Ala Thr 2515 2520 2525 Val Ile Asn Thr Ala Val His Phe Asn His Leu Phe Ser Leu Ser Gly 2530 2535 2540 Tyr Gln Trp Ile Leu Pro Thr Met Leu Gln Val Tyr Ser Asp Tyr Glu 2545 2550 2555 2560 Ser Asn Pro Gln Leu Arg Gln Ala Ile Glu Phe Ala Cys His Gln Phe 2565 2570 2575 Tyr Ile Leu His Arg Lys Pro Phe Val Leu Gln Leu Phe Ala Ser Val 2580 2585 2590 Ala Pro Leu Leu Glu Phe Pro Asp Ala Ala Asn Asn Gly Pro Ser Lys 2595 2600 2605 Gly Val Ser Ala Gln Cys Leu Phe Asp Leu Leu Gln Ser Leu Glu Gly 2610 2615 2620 Glu Thr Thr Asp Ile Leu Asp Ile Leu Glu Leu Val Lys Ala Glu Lys 2625 2630 2635 2640 Pro Leu Lys Ser Leu Asp Phe Cys Tyr Gly Asn Glu Asp Leu Thr Phe 2645 2650 2655 Ser Ile Ser Glu Ala Ile Lys Leu Cys Val Thr Val Val Ala Tyr Ala 2660 2665 2670 Pro Glu Ser Phe Arg Ser Leu Gln Met Leu Met Val Leu Glu Ala Leu 2675 2680 2685 Val Pro Cys Tyr Leu Gln Lys Leu Lys Arg Gln Thr Ser Gln Val Glu 2690 2695 2700 Thr Val Pro Ala Ala Arg Glu Glu Ile Ala Ala Thr Ala Ala Leu Ala 2705 2710 2715 2720 Thr Ser Leu Gln Ala Leu Leu Tyr Ser Val Glu Val Leu Thr Arg Pro 2725 2730 2735 Met Thr Ala Pro Gln Met Ser Arg Cys Asp Gln Gly His Lys Gly Thr 2740 2745 2750 Thr Thr Ala Asn His Thr Met Ser Ser Gly Val Asn Thr Arg Tyr Gln 2755 2760 2765 Glu Gln Gly Ala Lys Leu His Phe Ile Arg Glu Asn Leu His Leu Leu 2770 2775 2780 Glu Glu Gly Gln Gly Leu Pro Arg Glu Glu Leu Asp Glu Arg Ile Ala 2785 2790 2795 2800 Arg Glu Glu Phe Arg Arg Pro Arg Glu Ser Leu Leu Asn Ile Cys Thr 2805 2810 2815 Glu Phe Tyr Lys His Cys Gly Pro Arg Leu Lys Ile Leu Gln Asn Leu 2820 2825 2830 Ala Gly Glu Pro Arg Val Thr Ala Leu Glu Leu Leu Asp Val Lys Ser 2835 2840 2845 His Met Arg Leu Ala Glu Ile Ala His Ser Leu Leu Lys Leu Ala Pro 2850 2855 2860 Tyr Asp Thr Gln Thr Met Glu Ser Arg Gly Leu Arg Arg Tyr Ile Met 2865 2870 2875 2880 Glu Met Leu Pro Ile Thr Asp Trp Thr Ala Glu Ala Val Arg Pro Ala 2885 2890 2895 Leu Ile Leu Ile Leu Lys Arg Leu Asp Arg Met Phe Asn Lys Ile His 2900 2905 2910 Lys Met Pro Thr Leu Arg Arg Gln Val Glu Trp Glu Pro Ala Ser Asn 2915 2920 2925 Leu Ile Glu Gly Val Cys Leu Thr Leu Gln Arg Gln Pro Ile Ile Ser 2930 2935 2940 Phe Leu Pro His Leu Arg Ser Leu Ile Asn Val Cys Val Asn Leu Val 2945 2950 2955 2960 Met Gly Val Val Gly Pro Ser Ser Val Ala Asp Gly Leu Pro Leu Leu 2965 2970 2975 His Leu Ser Pro Tyr Leu Ser Pro Pro Leu Pro Phe Ser Thr Ala Val 2980 2985 2990 Val Arg Leu Val Ala Leu Gln Ile Gln Ala Leu Lys Glu Asp Phe Pro 2995 3000 3005 Leu Ser His Val Ile Ser Pro Phe Thr Asn Gln Glu Arg Arg Glu Gly 3010 3015 3020 Met Leu Leu Asn Leu Leu Ile Pro Phe Val Leu Thr Val Gly Ser Gly 3025 3030 3035 3040 Ser Lys Asp Ser Pro Trp Leu Glu Gln Pro Glu Val Gln Leu Leu Leu 3045 3050 3055 Gln Thr Val Ile Asn Val Leu Leu Pro Pro Arg Ile Ile Ser Thr Ser 3060 3065 3070 Arg Ser Lys Asn Phe Met Leu Glu Ser Ser Pro Ala His Cys Ser Thr 3075 3080 3085 Pro Gly Asp Ala Gly Lys Asp Leu Arg Arg Glu Gly Leu Ala Glu Ser 3090 3095 3100 Thr Ser Gln Ala Ala Tyr Leu Ala Leu Lys Val Ile Leu Val Cys Phe 3105 3110 3115 3120 Glu Arg Gln Leu Gly Ser Gln Trp Tyr Trp Leu Ser Leu Gln Val Lys 3125 3130 3135 Glu Met Ala Leu Arg Lys Val Gly Gly Leu Ala Leu Trp Asp Phe Leu 3140 3145 3150 Asp Phe Ile Val Arg Thr Arg Ile Pro Ile Phe Val Leu Leu Arg Pro 3155 3160 3165 Phe Ile Gln Cys Lys Leu Leu Ala Gln Pro Ala Glu Asn His Glu Glu 3170 3175 3180 Leu Ser Ala Arg Gln His Ile Ala Asp Gln Leu Glu Arg Arg Phe Ile 3185 3190 3195 3200 Pro Arg Pro Leu Cys Lys Ser Ser Leu Ile Ala Glu Phe Asn Ser Glu 3205 3210 3215 Leu Lys Ile Leu Lys Glu Ala Val His Ser Gly Ser Ala Tyr Gln Gly 3220 3225 3230 Lys Thr Ser Ile Ser Thr Val Gly Thr Ser Thr Ser Ala Tyr Arg Leu 3235 3240 3245 Ser Leu Ala Thr Met Ser Arg Ser Asn Thr Gly Thr Gly Thr Val Trp 3250 3255 3260 Glu Gln Asp Ser Glu Pro Ser Gln Gln Ala Ser Gln Asp Thr Leu Ser 3265 3270 3275 3280 Arg Thr Asp Glu Glu Asp Glu Glu Asn Asp Ser Val Ser Met Pro Ser 3285 3290 3295 Val Val Ser Glu Gln Glu Ala Tyr Leu Leu Ser Ala Ile Gly Arg Arg 3300 3305 3310 Arg Phe Ser Ser His Val Ser Ser Met Ser Ala Pro Gln Ala Glu Val 3315 3320 3325 Gly Met Leu Pro Ser Gln Ser Glu Pro Asn Val Leu Asp Asp Ser Gln 3330 3335 3340 Gly Leu Ala Ala Glu Gly Ser Leu Ser Arg Val Ala Ser Ile Gln Ser 3345 3350 3355 3360 Glu Pro Gly Gln Gln Asn Leu Leu Val Gln Gln Pro Leu Gly Arg Lys 3365 3370 3375 Arg Gly Leu Arg Gln Leu Arg Arg Pro Leu Leu Ser Arg Gln Lys Thr 3380 3385 3390 Gln Thr Glu Pro Arg Asn Arg Gln Gly Ala Arg Leu Ser Thr Thr Arg 3395 3400 3405 Arg Ser Ile Gln Pro Lys Thr Lys Pro Ser Val Asp Gln Lys Arg Ser 3410 3415 3420 Val Thr Phe Ile Glu Ala Gln Pro Glu Pro Ala Ala Ala Pro Thr Asp 3425 3430 3435 3440 Ala Leu Pro Ala Ala Gly Gln Leu Gln Gly Cys Ser Pro Ala Pro Ser 3445 3450 3455 Arg Lys Pro Glu Ala Arg Asp Glu Pro Val Leu Thr Ser Ser Pro Ala 3460 3465 3470 Ile Val Val Ala Asp Leu His Ser Val Ser Pro Lys Gln Ser Glu Asn 3475 3480 3485 Phe Pro Thr Glu Glu Gly Glu Lys Glu Glu Asp Thr Glu Ala Gln Gly 3490 3495 3500 Ala Thr Ala His Ser Pro Leu Ser Thr Gln Leu Ser Asp Pro Asp Asp 3505 3510 3515 3520 Phe Thr Gly Leu Glu Thr Ser Ser Leu Leu Gln His Gly Asp Thr Val 3525 3530 3535 Leu His Ile Ser Glu Glu Asn Gly Met Glu Asn Pro Leu Leu Ser Ser 3540 3545 3550 Gln Phe Pro Phe Thr Pro Thr Glu Leu Gly Lys Thr Asp Ala Val Leu 3555 3560 3565 Asp Glu Ser His Val 3570 <210> 203 <211> 265 <212> PRT <213> Artificial Sequence <220> <223> Snail1 XP_004451686.1 <400> 203 Met Pro Arg Ser Phe Leu Val Arg Lys Ser Ala Cys Ser Arg Arg Arg 1 5 10 15 Pro Lys Tyr Ser Glu Leu His Tyr Ser Ser Leu Asp Phe Ala Phe Gln 20 25 30 Ala Pro Cys Asp Gln Ala His Met Leu Ala Ala Val Ala Pro Pro Glu 35 40 45 Val Leu Asn Ser Thr Ala Ser Leu Pro Thr Leu Ile Trp Asp Ser Phe 50 55 60 Leu Ala Ser His Thr Gln Thr Val Gly Trp Ala Ser Leu Leu Pro Pro 65 70 75 80 Glu Gly Arg Lys Ala Ala Ala Ala Asp Leu Thr Ser Leu Ser Gly Gln 85 90 95 Asp Ser Gly Lys Gly Ser Gln Pro Leu Ser Pro Pro Ser Pro Ala Pro 100 105 110 Ser Ser Ser Ser Ala Ser Ala Ser Ser Pro Glu Ala Lys Thr His Ala 115 120 125 Thr Phe Leu Ser Leu Asp Gln Pro Pro Lys Gln Leu Thr Val Leu Thr 130 135 140 Val Ala Lys Asp Pro Gln Ser Pro Thr Ala Leu Ser Cys Lys Tyr Cys 145 150 155 160 Asp Lys Glu Cys Val Ser Leu Gly Ala Leu Lys Met His Ile Arg Asp 165 170 175 His Thr Leu Pro Cys Val Cys Asp Thr Cys Gly Lys Ala Phe Ser Arg 180 185 190 Pro Trp Leu Leu Arg Gly His Val Arg Thr His Thr Gly Glu Lys Pro 195 200 205 Tyr Ser Cys Ser His Cys Ser Arg Val Phe Ala Asp Arg Ser Asn Met 210 215 220 Arg Ala His Leu Gln Thr His Leu Asp Val Lys Lys Tyr Gln Cys Lys 225 230 235 240 Ala Cys Ser Arg Thr Phe Ser Arg Met Ser Leu Leu Arg Lys His Lys 245 250 255 Glu Leu Gly Cys Ser Gly Gly Pro Cys 260 265

Claims (9)

  1. (a) EPRS(Glutamyl-prolyl-tRNA synthetase) 전체 또는 서열번호 2 내지 서열번호 6으로 이루어지는 군에서 선택된 어느 하나의 아미노산 서열을 가지는 이의 단편, snail 1 단백질 및 시험제제를 접촉시키는 단계; 및
    (b) 상기 EPRS와 snail 1의 결합수준의 변화를 측정하는 단계를 포함하는 EMT(epithelial-mesenchymal transition) 저해제 스크리닝 방법.
  2. 제1항에 있어서, 상기 EMT 저해제는 암 전이(cancer metastasis), 섬유성 질환(fibrotic disease), 신생혈관형성(angiogenesis), 당뇨병성 신증(diabetic renal nephropathy), 동종이식편 기능장애(allograft dysfunction), 백내장(cataract) 및 심장판막형성 결함(defects in cardiac valve formation)으로 이루어진 군에서 선택된 질환 또는 증상의 예방 또는 치료제인 것을 특징으로 하는 스크리닝 방법.
  3. 제2항에 있어서, 상기 암은 비소세포 폐암, 소세포 폐암, 흑색종, 백혈병, 대장암, 간암, 위암, 식도암, 췌장암, 담낭암, 신장암, 방광암, 전립선암, 고환암, 자궁경부암, 자궁내막암, 융모암, 난소암, 유방암, 갑상선암, 뇌암, 두경부암, 피부암, 림프종 및 재생불량성 빈혈, 담도암, 구강암, 복막암, 소장암 및 안종양으로 로 이루어진 군에서 선택되는 것임을 특징으로 하는 스크리닝 방법.
  4. 제2항에 있어서, 상기 섬유증은 신장 섬유증, 간 섬유증, 폐 섬유증, 피부 섬유증, 심장 섬유증, 관절 섬유증, 신경 섬유증, 근 섬유증 및 복막 섬유증으로 이루어진 군에서 선택되는 것임을 특징으로하는 스크리닝 방법.
  5. 제1항에 있어서, 상기 EPRS는 서열번호 1, 서열번호 25 내지 서열번호 123으 로 이루어지는 군에서 선택된 어느 하나의 아미노산 서열로 이루어진 것을 특징으로 하는 스크리닝 방법.
  6. 제1항에 있어서, 상기 snail 1은 서열번호 15, 서열번호 124 내지 서열번호 203으로 이루어지는 군에서 선택된 어느 하나의 아미노산 서열로 이루어진 것을 특징으로 하는 스크리닝 방법.
  7. 제1항에 있어서, 상기 시험제제는 단백질 폴리펩티드, 소유기 물질(Small organic molecule), 다당류(Polysaccharide), 폴리뉴클레오티드 등으로 이루어진 군에서 선택된 하나 이상임을 특징으로 하는 스크리닝 방법.
  8. 제1항에 있어서, 상기 스크리닝 방법은
    (c) 상기 (b) 단계에서 EPRS와 snail 1의 결합수준이 변화된 시험제제를 TGF-β1과 함께 snail 1을 발현하는 세포와 접촉시키고 상기 세포에서 EMT 억제 효과를 확인하는 단계를 추가로 포함하는 것을 특징으로 하는 스크리닝 방법.
  9. 제8항에 있어서, 상기 snail 1을 발현하는 세포는 정상 상피세포, 비소세포 폐암 세포, 소세포 폐암 세포, 흑색종 세포, 백혈병 세포, 대장암 세포, 간암 세포, 위암 세포, 식도암 세포, 췌장암 세포, 담낭암 세포, 신장암 세포, 방광암 세포, 전립선암 세포, 고환암 세포, 자궁경부암 세포, 자궁내막암 세포, 융모암 세포, 난소암 세포, 유방암 세포, 갑상선암 세포, 뇌암 세포, 두경부암 세포, 피부암 세포, 림프종 세포, 재생불량성 빈혈 세포, 담도암 세포, 구강암 세포, 복막암 세포, 소장암 세포, 안종양 세포, 신장 섬유증 세포, 간 섬유증 세포, 폐 섬유증 세포, 피부 섬유증 세포, 심장 섬유증 세포, 관절 섬유증 세포, 신경 섬유증 세포, 근 섬유증 세포 및 복막 섬유증 세포로 이루어지는 군에서 선택되는 것을 특징으로 하는 스크리닝 방법.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018150188A1 (en) * 2017-02-17 2018-08-23 Oxford Brookes University Screening method
EP3567119A4 (en) * 2017-01-09 2020-08-19 Met Life Sciences Co., Ltd. PHARMACEUTICAL COMPOSITION FOR THE TREATMENT OF PRE-ORAL CANCER AND METHOD FOR PREDICTING OR DIAGNOSING AN ORAL CANCER PRE-ORAL CANCER

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3567119A4 (en) * 2017-01-09 2020-08-19 Met Life Sciences Co., Ltd. PHARMACEUTICAL COMPOSITION FOR THE TREATMENT OF PRE-ORAL CANCER AND METHOD FOR PREDICTING OR DIAGNOSING AN ORAL CANCER PRE-ORAL CANCER
WO2018150188A1 (en) * 2017-02-17 2018-08-23 Oxford Brookes University Screening method

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