KR20160081042A

KR20160081042A - The method for preparing lacquer chicken sauce with Detoxified Stem Bark of Rhus verniciflua Extract by using Basidiomyces

Info

Publication number: KR20160081042A
Application number: KR1020140194205A
Authority: KR
Inventors: 은봉기
Original assignee: 한영선
Priority date: 2014-12-30
Filing date: 2014-12-30
Publication date: 2016-07-08

Abstract

The present invention relates to a method for preparing lacquer chicken meat using ricotta liquorice extract and chicken bone, and more particularly, to a method for producing health functional lacquer meat juice using ricotta extract and chicken bone which reduce urushiol component inducing allergen of Rhus verniciflua.

Description

Technical Field [0001] The present invention relates to a method for preparing a healthy functional lacquer chicken broth using detoxified ricotta extract,

The present invention relates to a method for preparing lacquer meat and broth using ricotta extract and chicken bone, and more particularly, to a method for producing health-functional lacquer broth using rheumatoid arthritis extract and chicken bone, which reduce urushiol component inducing allergies of Rhus verniciflua.

Lacquer tree has been widely used as a medicinal herb because it helps to digest digestion of gastrointestinal tract, blood eosinophilia and cardiopulmonary function, and is effective in the treatment of diabetes, gynecological diseases, insomnia, abdominal pain and anemia. It has also been used with traditional folk paintings and with chickens. The effects of antioxidant, anticancer, antibacterial, anti-toxic, anti-inflammatory, anti-thrombotic, anti-obesity, anti-diabetic, antirheumatic, anti-neurodegenerative , Cell protection, liver protection, and hangover resolution.

The main active ingredient of Rhus verniciflua is uriciol present in the bark, and it is known that it exhibits strong anti-cancer activity and antioxidant activity as well as immune enhancement activity in AIDS patients. On the other hand, the woody part of Rhus verniciflua, which consists of flavonoids such as fustin, fisetin, sulfuretin, butein, and garvanzol and many other substances such as adenosine, does not induce allergic reactions and exhibits various pharmacological actions. As a result of scientific research on the components and physiological activity of Rhus verniciflua, the availability of Rhus verniciflua has become more and more unlimited.

Although the lacquer tree has excellent physiological activity, various studies have been demanded because there are limitations due to ingredients such as urushiol which cause dermatitis.

Lacquer contains urushiol which causes contact dermatitis such as blisters, itching, and rash. However, it has been traditionally added to cook chicken and duck in order to improve poor circulation, diabetes and gastrointestinal symptoms. Chickens and ducks are natural neutralizers that release the toxicity of lacquer, and it is reported that digestion and absorption are good.

The present invention provides lacquer broth using a detoxified ricotta extract obtained by eliminating urushiol component. The present invention relates to a lacquer broth having high efficacy and convenience for eating, which is effective against gastritis and gastric ulcer, The purpose is to provide.

In addition, the use of food waste or disposable chicken bones generally provides an opportunity to further utilize the ingredients.

As a means for achieving the object of the present invention, a raspberry extract is prepared by removing urushiol. The raspberry extract is obtained by inoculating a mushroom strain into a medium in which grains and raspberry extract are mixed, and culturing at a predetermined temperature for a predetermined period. The washed chicken bone is mixed with egg yolk, egg yolk, Angelica gigas, and garlic, and the mixture is heated for a predetermined period of time to remove solid matter, followed by addition of the obtained raspberry extract, followed by heating and cooling to prepare lucerne broth.

Since the lacquer broth according to the present invention is provided, not only can the chicken pancake be instantly prepared, but also the urushiol of the lacquer is removed, so that the health-functional chicken pancake having no side effects due to lacquer can be obtained.

In addition, there is an effect that waste materials that are wasted by utilizing chicken bone can be effectively used.

FIG. 1 is a test graph showing the gastric-protective effect of raspberry extract on gastric damage model with hydrochloric acid-ethanol,
FIG. 2 is a graph showing the influence of ricin-extract on the amount of gastric juice in a gastric injury model using hydrochloric acid-ethanol,
FIG. 3 is a test graph showing the effect of RVSB extract on the pH of gastric juice in a gastric injury model with hydrochloric acid-ethanol, and
Figs. 4 and 5 are test graphs showing the inflammatory-related cytokine inhibitory action of raspberry extract.

Hereinafter, the present invention will be described in detail with reference to examples.

1. Preparation of raspberry extract:

First, Rhus verniciflua powder was mixed with rice selected from the group consisting of rice, brown rice, rice gruel, rice gruel, black rice, grains, barley, soybeans and mixtures thereof and heated at 110 캜 for 1 hour for sterilization, And cultured at a temperature of 15 to 37 DEG C for 7 to 30 days. In this way, the addition of cereal grains to the powdered mushroom can enhance the growth of mushroom bacteria and the detoxification of urushiol, because nutrient fortification and growth promoting substances are supplemented.

Examples of the mushroom microorganism include, but are not limited to, mushroom, mushroom, gingko mushroom, mushroom mushroom, mushroom mushroom, mushroom mushroom, Oyster mushroom, shiitake mushroom, rabbit hairpin mushroom, mandarin mushroom mushroom, fenugreek mushroom, mushroom mushroom, Tyromyces palustris, or a mixture thereof.

In addition, when cultivating for more than the designated period of time, the components of the lacquer can be taken away by the mushroom, while culturing for more than 7 days is sufficient to remove the toxicity of the lacquer.

Lactobacillus containing the cultured Lacq medium and mushroom mycelium is put in a solvent such as water and boiled for a predetermined time (85 ° C for 1 hour, 95 ° C for 2 hours, 100 ° C for 3 to 5 hours). The organic solvent may be water, methanol, acetone, ethanol, propanol, butanol, spirits alcohol or a mixed solvent thereof, but water, ethanol, or alcohol is more preferable as the extraction solvent in addition to water . It is usually extracted with an organic solvent for about 3 days. The water extract can be used by adding distilled water to about 10 times of the sample and then refluxing the sample for 3 hours at 100 ° C with a reflux condenser. The ethanol extract can be used as filter paper (No. 2) It is recommended to add 5 times the amount of ethanol (95%) and leave it for 10 days at room temperature. Filter paper (No. 2) should be used for the extraction. Acetone extract is added 10 times acetone, Filter paper (No. 2) is recommended for use. The solid component is filtered to obtain an extract solution containing the lacquer component.

Experimental results of the thus obtained raspberry extract are shown in Figs. 1 to 5.

That is, in the stomach damage test by hydrochloric acid ethanol, the RVOTE extract showed 22% and 36% significant inhibition of stomach damage, respectively, and it showed that the RVOX extract blocked the direct stimulation of the gastric mucosa of hydrochloric acid ethanol, It is believed that the wall of the

In addition, as shown in FIG. 2, as shown in FIG. 2, the rheumatoid arthritis extract reduces the amount of gastric juice secretion, thereby preventing stomach damage. In addition, by increasing the pH of gastric acid as shown in FIG. 3, the acidity of gastric acid is lowered, .

FIGS. 4 and 5 show that treatment with LPS (lipopolysaccharide), which is an inflammation inducer, on macrophage Raw264.7 and the effect of ruminal lacquer extract on the secretion of cytokines was confirmed to have a considerable inhibitory effect on inflammation as compared with the control group. This suggests that raspberry extract has anti-inflammatory activity.

2. Preparation of lacquer broth:

61.68% by weight of water, 20.56% by weight of the raspberry extract prepared above, 16.45% by weight of chicken bone,

0.62 wt.%, Angelica gangrene 0.31 wt.%, Hwanggi 0.21 wt.%, Rhizoctonia 0.1 wt.%, And 0.06 wt.% Of salt are placed in a vessel and heated at 100 to 120 ° C for 2 to 3 hours. At this time, if the amount of the additive added is too much, the functionality of the ralph grass extract is deteriorated.

If the heating time is short, the concentration is too dilute and the taste is not sufficient. If the heating time is long, it becomes like a jelly, and the problem that it is not loosened in the next step occurs.

The solids are removed from the heated mixture to obtain lacquer broth.

The results of the test on the lacquered chicken broth according to the present invention were as follows.

Lacock Helicobacter Pyory Antibacterial experiment

Helicobacter pylori strains were plated on a Brucella agar medium plate and 5 ml of concentrated lacZ broth was added to a sterilized paper disc approximately 8 mm in diameter and incubated at 37 ° C under 10% And the antimicrobial activity was confirmed. When treated with lacquer broth, it showed a clear zone of about 10 mm against Helicobacter pylori.

Treatment clear zone (mm) 12h 24h 48h Sterile water 0 0 0 Kanamycin (0.5 mg) 8.5 9.0 8.0 Concentrated lacquer chicken broth (5 mg) 9.5 10.5 10.0

In addition, when lacquer chicken meat according to the present invention was tested, urushiol was not detected at all.

Claims

A method for preparing healthy functional lacquer broth using ricotta extract,
The Rhus verniciflua powder is mixed with a predetermined grain and heated at 110 캜 for 1 hour to be sterilized, then inoculated with mushroom bacteria, cultured at a temperature of 15 to 37 캜 for a predetermined period of time, ;
20.56% by weight of the rquach extract prepared above, 61.68% by weight of water, 16.45% by weight of chicken bone,
Adding a mixture of 0.62 wt%, 0.31 wt% of Angelica gigasum, 0.21 wt% of Hwanggi, 0.1 wt% of Ganoderma lucidum and 0.06 wt% of salt into a vessel and heating the mixture at 100 to 120 캜 for a predetermined period of time; And
And removing the solids from the mixture to prepare lacquer broth. The method for producing health functional lacquer broth using the ricin extracts detoxified with porcine bacillus.

The method according to claim 1,
Wherein the grain is selected from the group consisting of rice, brown rice, brown rice, rice gruel, black rice, grains, barley, soybean, and mixtures thereof. .

The method according to claim 1,
The above-mentioned mushroom fungi to be used may be selected from the group consisting of longevity mushroom, gonorrhea mushroom, cormorant mushroom, mushroom mushroom, mushroom mushroom, oyster mushroom, mushroom mushroom, rabbit mushroom mushroom, mushroom mushroom mushroom, Wherein the method comprises the steps of: (a) preparing a solution of raloxifene Lactobacillus sp.

The method according to claim 1,
Wherein the predetermined period of time for culturing is from 7 days to 30 days. The method for producing health-functional lacquer broth using the ricin extracts detoxified with porcine bacillus.

The method according to claim 1,
Wherein the mixture is heated for 2 to 3 hours. The method of claim 1, wherein the rheobastain extract is a detergent.