KR20160075441A - Food Composition for Reducing Joint Pain Comprising Eucommia ulmoides Oliver, Achyranthes japonica Nakai, Sorbus commixta and Soybean Extract and Manufacturing Method There of - Google Patents

Abstract

One purpose of the present invention is to provide a food composition for joint pain relief, and the other purpose of the present invention is to provide a method for preparing the food composition for joint pain relief. A food composition for joint pain relief according to the present invention comprises 100 parts by weight of an eucommia bark extract, and 50 to 200 parts by weight of an Sorbus commixta extract, 50 to 200 parts by weight of an achyranthes extract and 100 to 300 parts by weight of a soybean extract based on 100 parts by weight of the eucommia bark extract. A method for preparing a food composition for joint pain relief according to the present invention comprises: a step (a) of preparing an ethanol extract by removing the ethanol after dipping eucommia bark, Sorbus commixta, achyranthes and soybean in the ground state into ethanol; a step (b) of preparing ethanol and a hot water extract by applying hot water to the ethanol extract of the step (a); a step (c) of preparing a hot water extract by adding again hot water to residue separated after preparing the ethanol and the hot water extract of the step (b); and a step (d) of mixing the ethanol and the hot water extract of the step (b) with the hot water extract of the step (c) at a weight ratio of 1 : 0.1-10 to prepare a mixture. A food composition for joint pain relief according to the present invention exhibits a substantial joint pain relief effect by proceeding a hot water extraction process again after performing an ethanol extraction process on target plants.

Description

[0001] The present invention relates to a food composition for alleviating joint pain, which comprises at least one compound selected from the group consisting of aldehyde,

The present invention relates to a food composition for alleviating joint pain, which comprises bug, fever, ridges and soybean extract, and more particularly to a mixture of ethanol extracts and hot water extracts of bugs, mums, raspberries and soya beans.

Osteoarthritis refers to a disease in which the cartilage layer developed within the joints of our body is damaged by friction between the bones and bones to relieve the impact and friction of bones and bones that directly strike, resulting in pain and difficulty in moving. Such osteoarthritis is a disease that can occur mainly from one joint such as knee, hip, hand, foot, spine, and may occur at the same time in various joints.

Rheumatoid arthritis is an autoimmune disease that causes inflammation by attacking the joints or parts of the body due to an abnormality of the immune system. The rheumatoid arthritis is a symptom of inflammation of the joints such as the fingers, hands, feet, wrists, ankles and knees, , Systemic chronic inflammatory disease that can cause abnormalities in various other organs such as skin, lungs, and eyes.

Since the direct cause of arthritis is not clear yet, researches on it have been continuing. To date, effective treatment methods and preventive measures for arthritis such as rheumatoid arthritis have not been well known. There are a lot of things to do.

Although many therapeutic agents and treatment methods have been suggested for the treatment of arthritis, these therapeutic agents and treatment methods are not considered to be the fundamental treatment methods, and hormonal agents such as steroid hormones have been widely known and used as therapeutic agents for arthritis. It became difficult to use it.

The composition for inhibiting osteoclast differentiation or a composition for promoting chondrocyte differentiation comprising a root extract and a local extract of chrysanthemum is disclosed in KR Patent Application No. 10-2010-0081478 And a composition for promoting chondrocyte differentiation,

KR Patent Publication No. 10-2011-0073400 (title of the invention: a method for the prevention and treatment of osteoarthritis or rheumatoid arthritis and a method for producing the same) discloses a method for preventing or treating osteoarthritis or rheumatoid arthritis, .

However, the technique included in the above-mentioned document is not effective enough to alleviate joint pain.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

The present inventors have tried to develop a composition capable of alleviating joint pain caused by arthritis or joint pain that may occur after normal work or exercise. As a result, the present inventors have found that the joint pain described above can be significantly reduced by taking the mixed extracts of mites, horses, ridges and legumes, thus completing the present invention.

Accordingly, an object of the present invention is to provide a food composition for alleviating joint pain.

It is still another object of the present invention to provide a method for producing the food composition for alleviating joint pain.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

The present invention provides a composition for alleviating joint pain.

The present inventors have tried to develop a composition capable of alleviating joint pain caused by arthritis or joint pain that may occur after normal work or exercise. As a result, it was confirmed that the above-mentioned joint pain can be remarkably reduced when taking the mixed extract of mites, woosk, japonica and western blot.

According to one aspect of the present invention, the present invention provides a composition comprising 100 parts by weight of an extract of Echinosophora koreensis, 50-200 parts by weight of Raspberry Extract, 50-200 parts by weight of Raspberry Extract, and 100-300 parts by weight of Soybean Extract A food composition for alleviating joint pain is provided.

The term " crushing " as used herein may mean breaking an object of a predetermined size finely, but it is not limited thereto and may mean an action of reducing an object size.

As used herein, the term " Duchin " may refer to a medicament that has dried bark of Eucommia ulmoides Oliver.

As used herein, the term " Seomyeongtae " is a perennial vine plant belonging to the soybean family, which may mean a seed of a plant having brown hairs on leaves and stems, and three small leaves on the end of long petiole.

As used herein, the term " Achyranthes " refers to perennial herbaceous plants or their roots belonging to the bacterium, and the term " acorn grass " to be. The general efficacy of the wax is that it works to smooth the blood circulation and remove the eosinophilia, and it is used for the treatment of physiological impurity, postpartum abdominal pain, and also works well in organs such as liver and kidney.

As used herein, the term "Sorbus commixta (SN)" is a deciduous broad-leaved arboreous arboreous tree, and is distributed naturally in the mountainous mountain range of 500-1200 m above sea level in Korea, China, and Japan. Bark of the legs is called as mahapi, and it is used for medicinal materials for protecting the kidneys in one room, bronchitis, paralytic gastritis and osteoporosis. The physiological activities of the rowan have been known as diabetic and obesity inhibitory effects, antioxidative and photoinhibitory effects, vascular inflammation inhibitory effects and atherosclerosis, and the constituents thereof include neosakuranin, lupeol, (lupenone) is known, but studies on the physiological activity in bone diseases are insufficient.

As a result of searching for KFDA food raw materials (http://fse.foodnara.go.kr), there is no problem in using as a pharmaceutical composition or a food composition legally, and also an excellent effect on blood circulation from the past Can be used in food or pharmaceutical compositions exhibiting joint pain relief and inflammation relief in light of what is known as a plant having an anti-inflammatory effect.

According to a preferred embodiment of the present invention, the phylloides preferably can be selected from the group comprising flowers, leaves, stems, fruits, seeds, roots and outpox, more preferably stem, May be a branch or shell of the stem.

According to a preferred embodiment of the present invention, the soybeans of the present invention can be preferably soybeans or black beans, more preferably black beans, and most preferably be seaweed.

The term " extract " used herein in reference to bivalves, beans, mosses, and Aspergillus extracts is intended to include not only extraction products obtained by treating the above-mentioned plants with an extraction solvent, but also formulations And the like.

If the extract used in the composition of the present invention is obtained by treating the plant with an extraction solvent, various extraction solvents may be used. (B) an anhydrous or a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, propanol, butanol, n-propanol, iso-propanol and n-butanol, (E) ethyl acetate, (f) chloroform, (g) 1,3-butylene glycol, (h) hexane, (i) diethyl ether, or (iii) a mixed solvent of a lower alcohol and water. (j) The extract can be obtained by treating the plant with butyl acetate.

As used herein, the term " extract " has the meaning conventionally used in the art as a crude extract, but broadly includes fractions obtained by further fractionation of the extract. That is, the plant extract includes not only those obtained by using the aforementioned extraction solvent, but also those obtained by further applying a purification process thereto. For example, a fraction obtained by passing the above extract through an ultrafiltration membrane having a constant molecular weight cut-off value, and a separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) The fraction obtained by the purification method is also included in the plant extract of the present invention.

The bug, raspberry, raspberry or soybean extract used in the present invention can be prepared in powder form by an additional process such as vacuum distillation and freeze drying or spray drying.

According to a preferred embodiment of the present invention, the extract of the present invention can be preferably a mixture of ethanol extract of hot pepper, moss, moss and soybean and hot water extract.

In the present invention, it is a very important constitution that the extract is a mixture of an ethanol extract and a hot water extract. This is because the use of a mixture of ethanol extract and hot-water extract of mosquito, wheat, horse mackerel and soybean significantly increases the joint pain relieving effect as compared with the ethanol extract or hot water extract alone of the above-mentioned bugs, Because it is possible to do so.

Using a mixture of ethanol extracts and hot-water extracts of mussel, wheat, rowan and soybeans increases the joint pain relief of about 20-30% compared to ethanol extracts of mussel, mussel, .

Herein, the mixture of the ethanol extract and the hot water extract is preferably prepared by dipping the above-mentioned bivalves, wheat, barley, and soybeans in ethanol for a certain period of time to extract and separate fat-soluble components of the bugs, And then immersing it in hot water to extract and isolate the water-soluble components of the above-mentioned bugs, wheat, rowan and soybean.

According to yet another aspect of the present invention, the present invention provides a method for preparing a food composition for alleviating joint pain comprising the steps of: (a) removing the ethanol after dipping the mosquito, barberry, To produce an ethanol extract; (b) adding hot water to the ethanol extract of step (a) to produce ethanol and hot water extract; (c) preparing a hot-water extract by adding ethanol and hot water extract of step (b) and adding hot water to the separated debris again; And (d) mixing ethanol and hot water extract of step (b) and hot water extract of step (c) to prepare a mixture.

Hereinafter, the method for manufacturing a food composition for alleviating joint pain will be described in detail.

Step (a): Preparation of ethanol extracts of mussel, ridges, cucumber and soybean

The first step is the production of ethanol extracts of mites, ridges, mosses and beans. The ethanol extract may be prepared by immersing the above-mentioned bugs, twigs, beans, and soybeans in ethanol for a predetermined time and then removing the ethanol.

According to a preferred embodiment of the present invention, the bamboo shoots, twigs, raspberries, beans and beans of the present invention can be preferably in a pulverized state.

According to a preferred embodiment of the present invention, the submerging immersion time of the present invention may be preferably 1-30 hours, more preferably 5-23 hours, and most preferably 15-20 hours. have.

Herein, the bamboo shoots, twig, and twig can be crushed by a crusher after the drying step, but the beans can be crushed after roasting for 3-5 minutes.

According to a preferred embodiment of the present invention, the frequency of the ethanol of the present invention may preferably be 30-99 degrees.

The ethanol can be removed by heat, preferably by evaporating the ethanol, and most preferably by heating to 50-70 [deg.] C.

Step (b): Preparation of ethanol and hot-water extracts of mussel, acorn, bean and soybean

The second step is the production of ethanol and hot water extracts of mites, ridges, marsh and soybean. The ethanol and the hot water extract may be prepared by removing hot water from the ethanol extract of step (a) and then removing the debris.

In the present invention, the process of preparing ethanol and hot water extract by adding hot water to the ethanol extract of step (a) is very important. Ethanol extraction, and the joint pain relief effect can be dramatically improved.

According to a preferred embodiment of the present invention, the hot water temperature of the present invention may preferably be 90-100 占 폚, and the hot water extraction time of the present invention may be preferably 1-20 hours.

Step (c): Preparation of hot-water extracts of mung bean, rowan, whisker and bean

The third step is the production of hydrothermal extracts of mites, ridges, mosses and beans. The hot-water extract may be prepared by preparing ethanol and hot-water extract of step (b), and adding hot water to the separated debris again.

In the present invention, the step of preparing the hot water extract by adding hot water to the remnants prepared by separating ethanol and hot water extract of step (b) is a very important constitution. In the process of preparing the hot-water extract, it is possible to extract most of the substances associated with the joint pain relief pharmacological activities that were not extracted during the steps (a) and (b).

According to a preferred embodiment of the present invention, the residue of the present invention may be in a dry state.

Step (d): Preparation of the mixture

The fourth step is to mix the ethanol and hot water extract of step (b) and the hot water extract of step (c) to prepare a mixture.

According to a preferred embodiment of the present invention, the ethanol and hot water extract of step (b) of the present invention and the hot water extract of step (c) may be mixed in a ratio of preferably 1: 0.1-10 on a weight basis.

According to a preferred embodiment of the present invention, the solids content of the mixture of step (d) of the present invention may preferably be 2-10% by weight, and most preferably 3-5%.

The composition of the present invention can be used mainly in a food composition for alleviating joint pain, but it can be used in a pharmaceutical composition because it has a joint pain relieving effect and an inflammation relieving effect and can be applied to a joint region, It can not be excluded from use in the composition.

According to a preferred embodiment of the present invention, the composition of the present invention may be a pharmaceutical composition having excellent joint pain relieving effect and anti-inflammatory effect.

When the composition of the present invention is manufactured from a pharmaceutical composition, the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier. The pharmaceutically acceptable carriers to be contained in the pharmaceutical composition of the present invention are those conventionally used in the formulation and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, But are not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrups, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. It is not. The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc. in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans in various routes such as oral or parenteral routes such as oral, rectal or intravenous, muscular, subcutaneous, intra-uterine, Can be administered by injection. Preferably, it is applied by transdermal administration during parenteral administration, more preferably by topical application by application.

The appropriate dosage of the pharmaceutical composition of the present invention may vary depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate, . The dose of the pharmaceutical composition of the present invention can be administered in an amount of 0.1-100 mg / kg on an adult basis once or several times a day in the case of an oral formulation, and in the case of an external preparation, It is preferable to apply it once to 5 times a day in an amount of 3.0 ml and continue for 1 month or longer. However, the dosage is not intended to limit the scope of the present invention.

The pharmaceutical composition of the present invention may be formulated into a unit dose form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art to which the present invention belongs. Or by intrusion into a multi-dose container. The formulations may be in any form suitable for pharmaceutical preparations including oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, external preparations such as ointments and creams, suppositories and sterile injectable solutions, , Dispersants, or stabilizers.

According to a preferred embodiment of the present invention, the composition of the present invention may be a food composition excellent in joint pain relief and anti-inflammatory effect.

When the composition of the present invention is prepared with a food composition, it includes not only the active ingredient, but also ingredients normally added during the manufacture of the food, including, for example, proteins, carbohydrates, fats, nutrients, flavoring agents and flavoring agents . Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings.

For example, when the food composition of the present invention is prepared as a drink, it may further contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, mulberry extract, jujube extract, licorice extract, have.

According to an embodiment of the present invention, the cosmetic composition of the present invention is obtained from the group comprising glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin Further comprising at least one auxiliary component selected from the group consisting of glycerin, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, squalane and sodium citrate, and at least one auxiliary ingredient selected from the group consisting of butylene glycol, citric acid, panthenol and allantoin And most preferably at least one component selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin .

Since the cosmetic composition of the present invention is basically applied to the skin, it can be provided with reference to the cosmetic composition of the related art, for example, as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, But are not limited to, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

As a result of analyzing cell viability with respect to the active ingredient of the composition of the present invention in the concrete examples of the present invention, it was found that the active ingredient of the composition of the present invention is a harmless substance as a natural substance. Therefore, since the active ingredient of the present invention has little toxicity and side effects, it can be safely used even for long-term use, and can be safely applied to cosmetic, pharmaceutical and food compositions as described above.

The features and advantages of the present invention are summarized as follows:

(a) 100 parts by weight of the extract of Echinacea, 50-200 parts by weight of Raspberry Extract, 50-200 parts by weight of Raspberry Extract, and 100-300 parts by weight of Soybean Extract, A food composition for relaxation is provided.

(b) The food composition for relieving joint pain of the present invention exhibits remarkable joint pain relieving effect by subjecting plants to hot water extraction process after ethanol extraction.

1 shows a specific manufacturing process of the food composition for relieving joint pain according to the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

Example

Throughout this specification, "%" used to denote the concentration of a particular substance is intended to include solids / solids (wt / wt), solid / liquid (wt / The liquid / liquid is (vol / vol)%.

Preparation Example: Preparation of mixed extract

Production Example 1: Preparation of mulberry extract

Ethanol extract

1 kg of mites were washed several times with a washing machine to remove foreign matter by using purified water, dried using a drier, and then pulverized. Then, the pulverized bugs were placed in an extraction container, and ethanol (38%) having a weight of 6 times (v / w) of the bivalves was placed in the extraction container and aged at room temperature for 10 days to prepare a bug extract. Finally, an appropriate amount of purified water was added to the extract, and then the ethanol component was separated and discharged at 60 ° C for 1-10 hours to prepare an ethanol extract.

Ethanol and hot water extract

Purified water was added to the ethanol extract to adjust the solid content to 3.5-4.5% by weight to prepare a mixture. Then, the mixture was heated at 90-100 ° C for 5-15 hours, and ethanol and hot water extracts and residue were separated using a filter net to prepare ethanol and hot water extract.

Jungchun hot water extract

The residue produced during the production of the ethanol and hot water extract was dried. Then, purified water was added to the dried residue to adjust the solid content to 3.5-4.5% by weight to prepare a mixture. Then, the mixture was heated at 90-100 ° C for 5-15 hours, and the hot water extract and the residue were separated using a filter net to prepare a hot water extract.

Mulberry blend extract

The mixture of the above-mentioned dihydroxy ethanol and the hot-water extract and the dicotyledonous hot-water extract was mixed at a ratio of 1:

Production Example 2: Preparation of Rectal Extract

Rectangular ethanol extract

500 g of branches and 500 g of stem were washed several times with a washing machine to remove foreign matter by using purified water, dried using a drier and then pulverized. Then, the pulverized asparagus was placed in an extraction container, ethanol (38%) having a weight of 6 times (v / w) was added to the extraction container, and the mixture was aged at room temperature for 10-15 days. Finally, an appropriate amount of purified water was added to the Rectilinear Extract, which was then heated at 60 ° C for 1 to 10 hours to separate and extract the ethanol component to prepare an ethanol extract.

Rectangular ethanol and hot water extract

Purified water was added to the ethanol extract to adjust the solid content to 3.5-4.5% by weight to prepare a mixture. Then, the mixture was heated at 90-100 ° C for 5-15 hours, and then ethanol and hot water extract and residue were separated using a filter net to prepare a mixture of ethanol and hot water.

Rowan Extract

The residue produced during the production of the ethanol and hot water extract was dried. Then, purified water was added to the dried residue to adjust the solid content to 3.5-4.5% by weight to prepare a mixture. Thereafter, the mixture was heated at 90-100 ° C for 5-15 hours, and then the hot water extract and the residue were separated using a filter net to prepare hot water extract of R.

Rectangular mixed extract

The Rowanberry ethanol and hot water extract and the Rowanberry hot water extract were mixed at a ratio of 1: 1 by weight to prepare a Rowanberry mixed extract.

Production Example 3: Preparation of a spinach extract

Ethanol extract

1 kg of wheat was washed several times with a washing machine so that there was no foreign substance by using purified water, dried using a drier, and then pulverized. Then, the pulverized whisk was placed in an extraction container, ethanol (38%) having a weight of 6 times (w / w) of the weight of the whisk was placed in the extraction container, and aged at room temperature for 10-15 days to prepare a whey extract. Finally, an appropriate amount of purified water was added to the writh extract solution, and the mixture was heated at 60 ° C for 1 to 10 hours to separate and discharge the ethanol component to produce ethanol extract.

Whey Ethanol and Hot Water Extract

Purified water was added to the ethanol extract to adjust the solid content to 3.5-4.5% by weight to prepare a mixture. Thereafter, the mixture was heated at 90-100 ° C for 5-15 hours, and ethanol and hot water extract and residue were separated using a filter net to produce ethanol and hot water extract.

Waxy hot water extract

The residue produced during the production of the ethanol and hot water extract was dried. Then, purified water was added to the dried residue to adjust the solid content to 3.5-4.5% by weight to prepare a mixture. Then, the mixture was heated at 90-100 ° C for 5-15 hours, and then the hot-water extract and the residue were separated using a filter net to prepare a hot-water extract of tsunami.

Mixed extract of moss

The above-mentioned waxy ethanol and hot-water extract and the waxy hydrothermal extract were mixed at a ratio of 1: 1 on the weight basis to prepare a mixed solution of mist.

Production Example 4: Preparation of a dry extract of soybean

Wheat bean ethanol extract

1 kg of bean curd beans was washed several times with a washing machine so that there was no foreign substance by using purified water, dried using a drier, and roasted for about 3-5 minutes. Then, the ground beans were placed in an extraction container, ethanol (38%) 6 times (v / w) by weight of the beans was added to the extraction container, and aged at room temperature for 10-15 days to prepare a soybean extract. Finally, an appropriate amount of purified water was added to the soybean extract, and then the ethanol component was separated and discharged at 60 ° C for 1-10 hours to prepare a crude soybean ethanol extract.

* Wheat bean ethanol and hot water extract

Purified water was added to the ethanol extract to adjust the solid content to 3.5-4.5% by weight to prepare a mixture. Then, the mixture was heated at 90-100 ° C for 5-15 hours, and ethanol and hot water extracts and residue were separated using a filter net to prepare soybean ethanol and hot water extract.

Extract of bean hot water extract

* The residue produced during the production of ethanol and hot water extract was dried. Then, purified water was added to the dried residue to adjust the solid content to 3.5-4.5% by weight to prepare a mixture. Then, the mixture was heated at 90-100 ° C for 5-15 hours, and then the hot-water extract and the residue were separated using a filter net to prepare a hot-water extract of bean curd.

Mixed extract of snow bean

The mixture of soybean ethanol and hot water extract and the hot-water extract of wild-bean soybean was mixed at a ratio of 1: 1 on the weight basis to prepare a mixed extract of wild bean.

Preparation Example 5: Preparation of mixed extract

100 parts by weight of the moss-mixed extract, 100 parts by weight of the moss-mixed extract, 150 parts by weight of the mixed moss-like extract, 180 parts by weight of the mossy mixed extract and 200 parts by weight of the mixed extract of Seomyeongbyeol were mixed by a mixer to complete an extract (Cooling temperature is -50 ° C to -40 ° C, drying temperature is 60 ° C or more at room temperature, and the cooling and drying time is about In case of 1 ton, automatic program operation takes 3 ~ 4 days.) After sterilization, powder is commercialized (solid content 3.5-4.5%).

Experimental Example: Confirmation of joint pain relief and anti-inflammatory effect

Experimental Example 1: Confirmation of joint pain relieving effect

The effect of alleviating joint pain on the food composition of the present invention was verified. We selected 20 experimental subjects who complained of severe knee pain among adult males and females over 50 years old living in Jecheon, Chungcheongbuk-do. The subjects were asked about knee pain for about 1 year and 6 months from April 2013 to October 2014 Each 1000 mg of the composition of the present invention was used to test the effect of alleviating knee pain.

1000 mg of the composition of Example 1 was administered to the above subjects three times in total, and as a comparative example, 100 parts by weight of the mucilage ethanol extract, 150 parts by weight of the municipal ethanol extract, 150 parts by weight of ethanol, 180 parts by weight of the extract and 200 parts by weight of the bean ethanol extract were mixed and then the lyophilized composition was administered once in 1000 mg portions three times in total.

As a result of the test, the composition of Example 1 showed remarkably excellent knee pain relieving effect as compared with the composition of Comparative Example. Notably, in the case of the composition of Example 1, even when the composition was dosed at a dose of 50-70% as compared with the weight of the composition of the Comparative Example, the same effect as that of the composition of the Comparative Example was exerted. These results suggest that the effect of hot water extraction on the ethanol extract of bean japonica, japonica japonica, wheat, and twigs was increased in the knee pain relief.

Experimental Example 2: Measurement of 5-LO and COX-2 inhibitory activity

5-Lipoxygenase (hereinafter referred to as 5-LO) is a substance that converts arachidonic acid into leukotrienes, known as inflammatory mediators, and is one of the mediators that aggravate inflammation. The 5-LO inhibitory activity of the composition prepared in Example 1 was assayed using an assay kit (lipoxygenase inhibitor screening assay kit). 90 μL of 5-LO (220 units / mL) and 10 μL of 1 mM arachidonic acid were added to 10 μL of the composition prepared in Example 1, and reacted at room temperature for 5 minutes. Then, 100 μL of a chromogen And incubated at room temperature for 5 minutes. Absorbance was measured at 490 nm using an enzyme-linked immunosorbent assay (ELISA) autoreader. The inhibition rate was calculated using nordihydroguaiaretic acid (NDGA) as a positive control.

Cyclooxygenase-2 (COX-2) is known as an inflammatory factor. Example 1 The COX-2 inhibitory effect of the composition was tested using a COX inhibitor screening assay kit. 10 μL of hemo, 5 μL of enzyme, and 950 μL of buffer were added to 20 μL of the composition of Example 1, and reacted at 37 ° C. Then, 10 μL of arachidonic acid was added and reacted at 37 ° C. for 2 minutes to form COX-1 and COX- 2 was activated. Next, 50 μL of the sample was added to 200 μL of the color material, reacted at 25 ° C. for 90 minutes, and the absorbance was measured at 420 nm in an ELISA reader reader. Inhibition rates were calculated using indomethacin, one of the nonsteroidal anti-inflammatory drugs (NSAIDs), as a positive control.

As a result of the experiment, it was confirmed that the composition of Example 1 exhibited remarkably higher efficacy than the ethanol extract of japonica, japonica, wooshu, and japonica in the 5-LO and COX-2 inhibitory effects.

Experimental Example 3: PGE ₂  Production amount measurement

Prostaglandin E2 (PGE2) is secreted by the action of COX-2 and is known to be a factor that exacerbates inflammation of soft tissues. Murine macrophage cell line RAW 264.7 cells were purchased from Korean Cell Line Bank (KLCB) and cultured in DMEM supplemented with 10% FBS (Fetal Bovine Serum) and 1% antibiotic to measure the inhibitory effect of the composition of Example 1 on PGE ₂ production (Dulbecco's Modified Eagle's Medium) low glucose medium at 37 ° C in the presence of 5% CO ₂ for 2 to 3 days. Then, the content of PGE _{2 in} the RAW 264.7 cell culture was measured using an enzyme immunoassay kit. RAW 264.7 cells were divided into 96 well plates at a concentration of 1 × 10 ⁵ cells / mL using DMEM low glucose medium to prepare five samples. Lipopolysaccharide (Lipopolysaccharide), which is a bacterial inflammation inducer, (LPS) 100 ng / mL to induce inflammation. The four samples were treated with the composition of Example 1 at different concentrations (0, 62.5, 125, and 250 μg / mL, respectively) . After 24 hours, the cell culture supernatant was taken and the amount of PGE ₂ produced was measured.

As a result of the experiment, it was confirmed that the amount of PGE ₂ produced was significantly reduced in the composition of Example 1 than in the case of treatment with ethanol extracts of mulberry, western,

Experimental Example 4: Measurement of the decrease in expression of MMPs

Matrix metalloproteinases (MMPs) are known to cause destruction of the extracellular matrix (ECM) of the cartilage and joints, and gradually progress to diseases such as arthritis. It was determined whether the composition of Example 1 reduced MMPs expression.

As a result, it was confirmed that the expression of MMP-1, MMP-3 and MMP-13 protein and the expression of mRNA were significantly reduced in the composition of Example 1 as compared with the case of treatment with ethanol extract of mungbean,

Claims (3)

100 parts by weight of an extract of Echinacea, 150 parts by weight of an extract of Curcuma longa, 180 parts by weight of an extract of a persimmon, and 200 parts by weight of a soybean extract,
The extract is a mixture of an ethanol extract and a hot water extract,
Said Rake is a branch or shell of Rake,
The Eucommia ulmoides Oliver is a shell of Eucommia ulmoides Oliver,
The soybean is a bean,
A food composition for alleviating joint pain produced by the following steps:
(a) preparing a ethanol extract by immersing the bacterium in the pulverized state, the bean curd, the bean curd and the soybean in ethanol, and then removing the ethanol;
(b) adding hot water to the ethanol extract of step (a) to produce ethanol and hot water extract;
(c) preparing a hot-water extract by adding ethanol and hot water extract of step (b) and adding hot water to the separated debris again; And
(d) mixing ethanol and hot water extract of step (b) and hot water extract of step (c) in a ratio of 1: 0.1-10 by weight to prepare a mixture.
The method according to claim 1,
Wherein the ethanol frequency of step (b) is 30-99.9 degrees.
The method according to claim 6,
Wherein the solids content of the mixture of step (d) is 2-10% by weight.

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