KR20160054831A - Sex pheromone composition for attracting Apolygus lucorum (Meyer-Dur) and uses thereof - Google Patents

Abstract

The present invention relates to a sex pheromone composition for inducing Apolygus lucorum and a mating distrupter or a pheromone trap using the same, wherein the sex pheromone composition for inducing Apolygus lucorum comprises hexyl butyrate, (E)-2-hexenyl butyrate, (E)-4-oxo-2-hexenal, and (E)-2-octenyl butyrate. According to the present invention, provided is a sex pheromone composition for inducing Apolygus lucorum, which has a high effect in inducing Apolygus lucorum. Through a mating distrupter or a pheromone trap using the same, damage to fruits is reduced, and a used amount of insecticide in an orchard may be remarkably decreased.

Description

The present invention relates to a pheromone composition, and more particularly, to a pheromone composition for attracting Apolygus lucorum (Meyer-Dur)

The present invention relates to an artificial pheromone composition for the artificial lupine, which uses a sex pheromone component secreted by a females of the females, and a use thereof.

It is an insect belonging to the Yusa River of the Eucalyptus River. It is also known as a semi-insect, and it is living on the ground and aquatic life. The size of the body varies from the smallest 1.1mm (the stingless bristle) to the largest 65mm (the swimwear), and the shape of the body is flat plate-like (wide-necked), long-rod- And the wings are deformed / enlarged to give an unusual shape (shield worms). Most of the body color is green or dark brown and protects you from the enemy by using a protective color.

There are three types of yellowing materials: clown barb, long scissors horn, brown hawthorn, full color hawthorn, beech hawthorn, four spotted hawthorn, longevity hawthorn, kamibola hawthorn, red silkworm, The shape of the neck, the neck of the neck, the neck of the neck, the neck of the neck, the neck of the neck, the neck of the neck, the neck of the neck, the neck of the neck, There are more than 50,000 species worldwide, including blind nori.

These females, when mating is finished, usually put eggs on the leaves or stems of plants. In addition, they scatter on soil or stone gaps, or scatter on the backs of males like water jars. In addition, most of the nymphs and adult larvae both damage crops by sucking plant fruits, buds, flowers, or sap from their preferred plants.

Among the pests belonging to the blind, Miridae, the damages of fruit and flower crops by the genus Apolygus and Lygocoris are well known in foreign countries. In Korea, there are nine species of Apolygus, Lygocoris pabulinus (Linnaeus), Apolygus spinolae (Meyer-Dur) and Apolygus lucorum (Meyer-Dur) (L. idoneus (Linnavuori)) and 9 species in Lygocoris. These birds are scattered in the woodland, and the birds hatching in the spring of the following year after wintering hatch and damage the wintertime snow. A. spinolae and A. lucorum. Appear to occur nationwide and have a wide host range, which can be used to infect various crops such as grains, vegetables and tea.

Especially, it is known that green blight occurs more than twice a year, and the nymphs grow in asteraceae plants, especially in the vines. In the citrus orchard, it causes damage to the shoot shooter, which occurs mostly in the seedlings whose orchards are covered with weeds or covered with hay.

For example, the blind bean hatches in the grape snow, then hatches at the emergence of the grape leaves and attaches the new leaves, and continues to feed the new leaves until the blossoms come out, and the jujube leaves are first stuck , But the juvenile tissue is dead and the surrounding tissues continue to grow. Therefore, as the leaf grows, a large hole is formed and becomes deformed.

In Korea, these water - borne strains have emerged as important pests of fruit crops including rice, barley, soybean, and food crops and apples, persimmons, pears and grapes. Because they feed on the seeds and fruits of the crops by plowing them and feeding them, they are difficult to control by using insecticides because they have a great economic impact due to direct addition of agricultural products.

Behavioral control methods using manure traps using their pheromones and biological control methods using parasitic parasitic arthropods have been known as methods for controlling insecticides other than insecticides. In particular, these environmentally friendly pest control methods are attracting attention due to changes in consumption patterns of consumers who prefer safe agricultural products.

However, there is a problem that biological control by parasitic parasitic infestation is difficult to apply in Korea. This is due to the fact that most of the yellowing materials to be controlled are mainly generated from the non-crops, so that a large number of natural enemies are costly to grow and cost competitiveness is poor, and the natural enemies, This is because the effects are often inconsistent. Therefore, in Korea, it is difficult to mass-propagate and emit natural enemies in order to control the harmful substances. Therefore, the behavioral control method that attracts the nourishing materials using their pheromone is more advantageously used.

(E) -2 (E) -2-hexenylbutyrate (E) -2-hexenylbutyrate (E) -2-hexenylbutyrate -hexenyl butyrate) and (E) -4-oxo-2-hexenal (E) -4-oxo-2-hexenal . However, the composition of the sex pheromone according to the above method is a result of studies using females such as Lygus rugulipennis, Lygus pratensis, Lygocoris pabulinus, and the like, Of the male was not effective.

Until now, the incidence of Apolygus lucorum (Meyer-Dur) belonging to the Miridae is still unknown, so it is difficult to effectively report the occurrence of this pest and to control the environment.

 Michelle et al., Journal of Chemical Ecology Volume 40, Number 1, pp 71-83

SUMMARY OF THE INVENTION The present invention provides a composition capable of attracting males of green foxtail using a sex pheromone component of a foxtail female.

Another problem to be solved by the present invention is to provide an artificial pheromone composition which is capable of controlling at the same time as the attractant by further containing an insecticide together with the sex pheromone component.

Another problem to be solved by the present invention is to provide a pheromone trap and a mating disruptor capable of controlling the green blight by using the composition for attracting the green blubber.

In order to solve the above-mentioned problems, the present invention provides a process for producing (E) -2-hexenyl butyrate, (E) -2-hexenyl butyrate, (E) -4-oxo-2-hexenal) and (E) -2-octenylbutyrate (E) -2-octenyl butyrate.

The composition may further comprise an insecticide.

The present invention also provides pheromone traps and mating disrupters comprising the green blight-attractant pheromone composition.

According to the present invention, it is possible to provide an artificial pheromone composition having a high effect of inducing an artificial glutinous yellowing agent.

In addition, when the sex pheromone composition further includes an insecticide, it can exert an effective control against the insect pests by attracting and controlling the insect pests.

Through the mating disruptor and the pheromone trap for controlling the green blight of the present invention, it is possible to alleviate the fruit loss and significantly reduce the use amount of the pesticide in the orchard.

FIG. 1 is a result of gas chromatography / mass spectrometry (GC-MS) analysis of an extract of a female pheromone gland from a green blight.
Fig. 2 is a photograph showing male adult male blastocysts attracted to pheromone traps.

In the present invention, the sex pheromone of the green blight, which has not been disclosed so far, was first identified, and the analysis of the identified substance and the optimum composition ratio suitable for the blinding of the green blight were conducted to find a composition maximizing the attraction and control of the green blight As shown in FIG.

(E) -2-hexenyl butyrate, (E) -2-hexenyl butyrate, and (E) -2-hexenyl butyrate. The present invention relates to a sex pheromone composition for eco- (E) -4-oxo-2-hexenal) and (E) -2-octenyl butyrate (E) -2-octenyl butyrate. And a pheromone trap or mating disruptor using the same.

As described above, the three blends of hexyl butyrate, (E) -2-hexenyl butyrate and (E) -4-oxo-2-hexenal have been used as conventional glutamate luminescent pheromone compositions, , The addition of (E) -2-octenylbutyrate to the three sex pheromone components further attained the attractive effect of the green blotch.

(E) -2-hexenyl butyrate, (E) -4-oxo-2-hexenyl and (E) -2-octenyl butyrate preferably have a weight ratio of 5 To 20: 1.5 to 6: 0.5 to 2: 1 to 4, and when the weight ratio between the compositions is out of the above range, the effect of attracting green beans may be somewhat deteriorated. When the weight ratio of the hexyl butyrate, (E) -2-hexenyl butyrate, (E) -4-oxo-2-hexenyl and (E) -2-octenyl butyrate is 10: 3: It is more preferable because it exhibits the most excellent effect on the incentive of the green blind.

The green pheromone composition according to the present invention can be prepared in various formulations and used as an attractant for green foxtail. The formulations of the present invention may be in the form of any of the formulations conventionally manufactured in the art including, for example, solutions, emulsions, suspensions, emulsions, pastes, gels, creams, oils, powders, waxes, And the like, but the present invention is not limited thereto.

When the formulation of the present invention is a cream, additives such as a liquid diluent such as ethyl alcohol, water or propylene glycol, lanolin oil, acetylated lanolin alcohol, cetyl alcohol, stearyl alcohol, bis wax, BHT, alkyl Parabens and the like may be used. When the formulation of the present invention is in the form of gel, alkylene glycol, water, carbomer 940 and the like may be used as additives. When the formulation of the present invention is a spray agent, a liquid diluent such as water, ethyl alcohol or propylene glycol may be used as an additive.

In addition, the composition of the present invention may further include an insecticide capable of controlling or insecting the attractant-induced green mulberry. The insecticide is not particularly limited as long as it is a pesticide commonly used in the art, for example, sodium channel regulator, choline esterase inhibitor, neonitinoid, insoluble macrocyclic lactone, GABA-regulated chloride channel A bacteriostatic agent, a bacteriostatic agent, a bacteriocin inhibitor, a chitin synthesis inhibitor, an insect hormone mimetic, an octopamine receptor ligand, an ecdysone agonist, a ryanodine receptor ligand, a narethoxin analog, a mitochondrial electron transfer inhibitor, a lipid biosynthesis inhibitor, Viruses in the nucleopolyhedha which is a member of Bacillus thuringiensis, encapsulated delta-endotoxin of Bacillus thuringiensis, and natural or genetically modified virus insecticides.

Therefore, when the composition of the present invention further includes the above-described insecticide, it has an effect of not only attracting the green blotch but also insecticide at the same time.

On the other hand, the green blotch lyophobic pheromone composition can be used as a pest insect and an environmentally friendly antiseptic, and can be produced in the form of a pheromone trap or a mating disruptant containing the same.

When the pheromone lures (bait) suitable for male incentives are used to investigate the occurrence density of the green foxtail bark, the effect of controlling the foxtail can be improved by the red ginseng roots control.

In addition, when a large number of pheromone traps including the sex pheromone composition are installed in an orchard and a large number of male birds of green blight are caught, the probability of mating of females can be greatly reduced. At this time, when the sex pheromone composition further includes an insecticide, the insecticide is also excellent because the insecticide is buried in the male body attracted by sex pheromone.

When the mating disruptor is manufactured and prepared in a fruit orchard by using the composition of the present invention, it is possible to prevent the mating behavior of the adult mite Automatic control is possible.

Hereinafter, the present invention will be described in detail with reference to examples.

Example 1: Identification of sex pheromone component of females

The female obtuse female adult was caught, and the posterior chamber of the sex pheromone gland was cut with scissors and placed in an organic solvent (hexane) to obtain an extract. The obtained extract was identified by GC-MS equipped with DB-Waxetr (length 30 m) column.

As a result, as shown in FIG. 1, hexyl butyrate, (E) -2-hexenyl butyrate, (E) -2-hexenyl butyrate, (E) -4-oxo-2-hexenal) and (E) -2-octenylbutyrate ((E) -2-octenyl butyrate).

Example 2 Male Infertility Effect by Mixing Ratio of Sexual Pheromone Components

(E) -2-hexenyl butyrate, (E) -4-oxo-butyrate, and (E) -2-hexenyl butyrate identified in Example 1, (E) -2-octenyl butyrate was mixed at a weight ratio of 100: 30: 10: 20 to obtain a mixture of (E) -4-oxo-2-hexenal I looked at whether or not I could attract the male of the blind.

The experimental conditions are as follows.

(E) -2-hexenyl butyrate, (E) -4-oxo-butyric acid were added to 100ul of hexane, 2-hexenyl and (E) -2-octenylbutyrate were added and mixed as shown in the following Table 1, and the mixture was immersed in a polyethylene tube (10 cm) to prepare a bait. Each bait was inserted into a small wire in the middle of a delta-type sticky trap (275 mm  x 95 mm x 170 mm). The trap was hung on a vine branch about 1 m above the ground and examined for the number of male obtusa caught in the trap for about a week. The mean value of the number of green blinds caught in each trap is shown in the following Table 1 as the number of attracted birds (grains / traps).

(E) -2-hexenyl butyrate, (E) -4-oxo-2-hexenyl and (E) -2-ox The results are shown in Table 1 below, in the same manner as in Experimental Example 1, except that the amount of phenylbutyrate was added by the amount shown in Table 1 below.

hexyl butyrate
(㎍) (E) -2-hexenyl butyrate
(㎍) (E) -4-oxo-2-hexenal
(㎍) (E) -2-octenyl butyrate ([mu] g) Driftwood (grains / traps) Example 1 1,000 300 100 200 11.7a Example 2 1,000 300 200 200 9.0a Example 3 1,000 300 50 200 8.3a Comparative Example 1 1,000 300 100 0 1.3b Comparative Example 2 1,000 300 0 200 0c Comparative Example 3 1,000 0 100 200 0c Comparative Example 4 0 300 100 200 0c Comparative Example 5 0 0 0 0 0c

As shown in the above Table 1, in Experimental Examples 1 to 3 which included all of hexyl butyrate, (E) -2-hexenyl butyrate, (E) -4-oxo-2-hexenyl and (E) 3 showed that the lyophilized pheromone composition of the present invention is superior to the pheromone composition of Comparative Example 1, which does not contain (E) -2-octenylbutyrate, In the case of Comparative Examples 2 to 5 which did not contain at least one species, no attracting effect on the green blotch was observed.

Example 3: Effect of controlling pheromone trap

(E) -2-hexenyl butyrate, (E) -4-oxo-2-hexenyl and (E) -2-octenyl butyrate were prepared in a ratio of 10: 3: 1: 2 The pheromone trap was used as a bait in a grape orchard at a density of 50 densities per 1 hectare, and after 48 days, the number of male blossoms and green blurred The number of fruit damage caused by barley was investigated. As a control, the number of fruit damage was examined in a grape orchard without a pheromone trap nearby, and the effect of pheromone trap was evaluated. The results are shown in Table 2 below.

Driftwood (grains / traps) Fruit damage rate (%) Pheromone trap control 42.5 3.5 No protection - 12.0

As shown in Table 2, in the case of a grape orchard provided with a trap containing the sex pheromone composition according to Example 1, the fruit damage rate was significantly reduced compared with a grape orchard without a pheromone trap, and the effect of pheromone trap- .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the scope of the present invention is not limited to the disclosed exemplary embodiments but is to be construed as being limited only by the appended claims. Should be construed as being included in the scope of the present invention.

Claims (6)

Hexenyl butyrate, (E) -2-hexenyl butyrate, (E) -4-oxo-2-hexenyl ((E) -4-oxo-2- hexenal) and (E) -2-octenyl butyrate ((E) -2-octenyl butyrate). The method according to claim 1,
Hexenyl butyrate, (E) -2-hexenyl butyrate, (E) -4-oxo-2-hexenyl and (E) To 4% by weight of the composition. The method according to claim 1,
(E) -2-hexenyl butyrate, (E) -4-oxo-2-hexenyl and (E) -2-octenyl butyrate were mixed in a weight ratio of 10: 3: Lactic pheromone composition for. The method according to claim 1,
Wherein the composition further comprises an insecticide. 7. A pheromone trap comprising an agar-derived pheromone composition according to any one of claims 1 to 4. 7. A mating disruptor comprising the green peat herbicidal pheromone composition according to any one of claims 1 to 4.

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