KR20160025808A - Composition having effects of preventing or recovering fatigue or stress - Google Patents

Abstract

The present invention relates to a composition having both decursin and/or decursinol angelate, and an Angelica gigas Nakai extract, a rice embryo bud extract, and a dried radish extract as active ingredients for preventing or treating fatigue or stress. Animal test results of the composition in accordance with the present invention confirm that exercise time duration is significantly extended and lactate dehydrogenase (LDH), lactic acid and superoxide dismutase (SOD activity) in blood, which are the hematological indicator of fatigue and stress, are significantly decreased compared to those of the control group when a subject takes in the composition one time or for a long period. Furthermore, results of clinical trials on subjects with chronic fatigue syndrome indicate that the blood lactate concentration in body is reduced significantly if the subjects take in the composition one time or for a long period compared to that of the control group, and treatment and prevention of fatigue and stress are also confirmed in the survey. Therefore, the composition, according to the present invention, can induce recovery of fatigue and stress when a user takes in the composition one time, and prevention of fatigue and stress when the user takes in the composition for a long time, thereby having effects of: enhancing athletic performance, exhibiting the effect of preventing and treating fatigue by lowering the generation of lactic acid and LDH, which are caused by fatigue, and exhibiting the effect of preventing the development of stress by lowering SOD activity in blood. Additionally, the composition can be used safely in human beings since toxic experiments on animals indicate that the composition has no side effects.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for preventing or restoring fatigue or stress comprising decaginic and / or dexacin angelate or decursin and / or decascinol angelate as active ingredients, Composition having effects of preventing or recovering fatigue or stress}

The present invention relates to a composition for preventing or ameliorating fatigue or stress, and more particularly to a composition for preventing or restoring fatigue or stress, which comprises decaginic and / or dexacinol angelate or decaginic and / or dexacinol angelate as active ingredients, And a composition for prevention or restoration of fatigue or stress, which comprises an extract of rice husk and an extract of non-walnut gray as an active ingredient.

Fatigue can be classified into three categories: fatigue caused by physiological phenomena, fatigue caused by physical illnesses, and fatigue caused by mental causes. Causes of fatigue from physical illnesses include tuberculosis, hepatitis, hypothyroidism, insomnia, depression, anemia, alcoholism, diabetes, rheumatoid arthritis, sleep apnea syndrome, sleep apnea, systemic lupus erythematosus, multiple sclerosis, AIDS, cancer, and back pain. This fatigue can not be solved by a sufficient rest, and it has not been done so much for the whole day, but the fatigue of this condition must be treated with fundamental disease.

Most of the fatigue caused by excessive work or overwork is relieved after resting. This fatigue is a physical reaction that normally occurs in a healthy person, and is a physiological fatigue that appears in people who have worked hard. In modern people, more than 90% of chronic fatigue can be regarded as physiological and mental fatigue which is not associated with chronic diseases like the above. Nervous life and excessive stress in daily life cause chronic fatigue.

In general, fatigue is a condition in which the force required for muscle contraction activity is not sufficiently exercised, that is, a state in which exercise performance is reduced. In contrast to physical fatigue, stress is an imbalance of body rhythm due to mental overload . Therefore, a broad sense of fatigue is a concept that includes both general fatigue and stress, and is a reduction in the ability to do physical or mental activities. Specifically, fatigue refers to a state in which work efficiency deteriorates due to physical fatigue, and stress is a state in which mental fatigue causes homeostasis.

When exposed to fatigue and stress in the complex social life of modern society, fatigue and stress prevention and restoration are the main basis for daily life. When these fatigue and stress build up, it takes time to rest and regain physical strength. However, in the busy age of modern society, prevention of stress and prevention and recovery of fatigue are difficult. Accumulation of fatigue caused by overworking can lead to chronic fatigue, and may cause many diseases such as peptic ulcer, hypertension, diabetes and the like.

Recently, the importance of exercise has been emphasized in order to enjoy the leisure time of life with the well-being craze and to prevent and treat various adult diseases. As a result of this social interest, the athletes as well as the general public are increasingly in need of scientific food intake for improved exercise performance and effective fatigue recovery.

Accordingly, the present inventors have developed a new composition for preventing and restoring fatigue or stress.

The present invention provides a composition for preventing or recovering fatigue or stress, and a specific technical problem is as follows.

First, it is intended to provide a novel composition capable of effectively preventing and restoring fatigue or stress.

Second, it is intended to provide a novel composition for prevention or recovery of fatigue or stress without adverse effects on the human body.

In order to solve the above-mentioned technical problems, the present invention provides a method for the treatment and / or prevention of angiogenesis, which comprises administering to a mammal an effective amount of angiotensin converting enzyme inhibitor, angiotensin converting enzyme inhibitor, angiotensin converting enzyme inhibitor, Rice husk extract; And a non-waxy extract as an active ingredient. The present invention also provides a composition for preventing or restoring fatigue or stress.

Decarkin and / or decercinol angelate can be obtained from Angelica gigas by extraction, separation and purification. Specific extraction and purification methods are described in Ji Hyung Jun et al., Biochemical Journal, 1, 25-32 (1970) and Korean J. Pharmacogn., 21, 64-68 (1990)) and Patent Registration No. 10-2385092, but the present invention is not limited thereto. Any extraction method capable of obtaining dexacinol angelate is possible. Also, in the present invention, deckacin and / or decancinol angelate may be used in a state containing other substances added in the process for extracting Angelicae gigantis, such as salts, organic salts, complexes and the like. (Tetsuhiro Nemoto et al., Tetrahedron Letters, 41, 9569-9574 (2000); Lan Xie et al., J. Med. Chem , Patent Application No. 2000-25749 and Patent Registration No. 10-0417624), but the present invention is not limited thereto, and even if the synthesis conditions and the like are different, Any synthesis method capable of obtaining nol angelate can be used. On the other hand, the deacquin and / or decancinol angelate comprises an inorganic salt selected from the group consisting of sodium salt, potassium salt, magnesium salt and calcium salt or a salt of lysine, ethanolamine, N, N'-dibenzylethylenediamine Or a pharmaceutically acceptable salt such as an organic salt selected from the group consisting of

The rice gruel is preferably a hot-water extract extracted from purified water at 100 ° C or higher. More preferably, the rice husk is added to purified water having a concentration of 30-40 times and heated at 100 ° C or higher for 8-12 hours. Under such extraction conditions, various active ingredients including the rice husk can be extracted. The main active ingredients in rice ginseng include GABA (improving learning and memory capacity, reducing stress, improving liver function), octagolanol (physiological activity, improving endurance, decreasing body fat and increasing muscle mass), alpha-tocopherol ), Gamma oryzanol (Brain nutrition supply, Energy metabolism promotion, Obesity prevention), Dietary fiber (Enhancement of intestinal microorganism, Constipation prevention, Colonization activity function), Vitamin B1 Vitamin B6 (Promotion of Protein Nucleic Acid Metabolism, Anti-Aging, Neurological Disorders), Linoleic Acid (Skin Nutrition, Growth Promotion) ).

The non-salted extract is preferably a hot-water extract extracted from purified water at a temperature of 100 ° C or higher, more preferably a salted salmon is put into 500 to 600-fold purified water and heated at 100 ° C or higher for 8 to 12 hours for extraction. It contains iron, calcium, and dietary fiber. It is good for atopic, allergic skin, blood circulation, prevents aging of cells, has diuretic and anti-inflammatory action, and is good for digestion and absorption of intestinal waste.

The composition according to the present invention can be variously commercialized, and it can be applied to various health foods, pharmaceuticals, drinks, health drinks, cosmetics and the like, and also to all food and pharmaceuticals to which water is added. For example, a pharmaceutically acceptable carrier or a pharmaceutical additive, may be prepared as a pharmaceutical preparation, or may be prepared as a health functional food further including a pharmaceutically acceptable food additive. The present invention exemplifies a health functional drink containing at least one of diastase, GABA, Glutathione, aspartic acid, methionine, taurine, fructose, vitamin C, vitamin B, catechin, mineral, citric acid, herb flavor, sodium citrate and sweetener as food additives. . As the sweetener, brown sugar and / or enzyme-treated stevia may be selected.

According to the present invention, the following effects can be expected.

First, the composition according to the present invention was found to have a significantly longer exercise time than that of the control group for one or a long time of the composition, and the blood LDH, lactic acid, and SOD activity, which are hematological indexes of fatigue and stress, Respectively. As a result of human body test for persons who are fit for chronic syndrome, the concentration of lactic acid in the blood of the human body was significantly lower than that of the control group when the composition was administered once or over a long time, and the fatigue or stress questionnaire was restored and prevented . Thus, the composition according to the present invention not only improves exercise performance by inducing fatigue or stress recovery in a single dose, induces fatigue or stress prevention in a long-term use, lowers the production of lactic acid due to fatigue, Lowering the activity of preventing and restoring fatigue, and lowering the SOD activity in the blood to exert a stress-inhibiting effect.

Second, it is expected that the composition according to the present invention can be safely used in the human body since it has been confirmed that there is no adverse effect in the animal toxicity test.

1 is a graph showing changes in swimming time according to administration of a composition according to the present invention in an animal experiment.
FIG. 2 is a graph showing blood LDH changes according to administration of a composition according to the present invention in an animal experiment. FIG.
FIG. 3 is a graph showing changes in blood lactate according to administration of a composition according to the present invention in an animal experiment. FIG.
FIG. 4 is a graph showing blood SOD changes according to administration of a composition according to the present invention in an animal experiment. FIG.
FIG. 5 is a graph showing changes in the blood lactate concentration in the human body according to the ingestion of the composition according to the present invention in a human body experiment. FIG.
FIG. 6 is a graph showing the result of questionnaire evaluation of fatigue or stress phenomenon of an experimenter according to one composition ingestion according to the present invention in a human body experiment. FIG.
7 is a graph showing changes in swimming time according to the long-term administration of the composition according to the present invention in an animal experiment.
FIG. 8 is a graph showing blood LDH changes according to the long-term administration of the composition according to the present invention in animal experiments.
9 is a graph showing changes in blood lactic acid according to the long-term administration of the composition according to the present invention in an animal experiment.
10 is a graph showing changes in blood SOD according to the long-term administration of the composition according to the present invention in an animal experiment.
11 is a graph showing changes in blood lactate concentration in the human body according to the long-term consumption of the composition according to the present invention in a human body experiment.
12 is a graph showing the result of questionnaire evaluation of fatigue or stress phenomenon of an experimenter according to the long-term ingestion of the composition according to the present invention in a human body experiment.

Hereinafter, the present invention will be described in detail based on examples. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

[Example 1] Preparation of composition

The composition according to the present invention was mixed with a composition as shown in Table 1 below to prepare a beverage. The composition ranges below are the result of comprehensive consideration of functionality and sensibility.

The composition and composition ratio of the composition material Composition range Composition ratio Remarks Active ingredient The extract of Angelica gigas Nakai, which contains deckacin and / or decercinol angelate or deckacin and / or dexacinol angelate as active ingredients 0.01 to 10 0.8 The Korean red ginseng extract was cultivated and extracted according to the method described in Patent Registration No. 10-0385092 Rice eye extract 0.01 to 20 4 300 g of rice flour was added to 11000 g of purified water and the extract solution was extracted at 100 ° C. or higher for 10 hours Welsh onion extract 0.01 to 20 0.2 20 g of salted red wool was added to 11000 g of purified water, and the extract solution was extracted at 100 ° C. or higher for 10 hours additive Multivitamin 0.01 to 5 0.06 GABA, Glutathione, Aspartic acid, Methionine, Taurine, Fructose (honey), Vitamin C, Vitamin B, Catechin, Minerals, Sweetener, Citric acid, Herb incense, Sodium citrate sweetener 0.01 to 5 4 Brown sugar water 50 to 99.96 90.94 Purified water Sum 100 100 -

[Example 2] Fatigue or stress restorative effect

1. Animal experiments

(1) Experimental method

In a transparent plastic container of 25 × 40 × 17 cm, 15 cm of distilled water was filled in, and the swimming time was measured at 25 ± 1 ° C in the same time under the same conditions. To induce fatigue, 5% of the weight of the rats during swimming was fixed on the back of the neck of the rats. The end of swimming was settled for 5 seconds until the nose was submerged in the water, until the sinking occurred. At that time, the experiment was terminated. The experiment was carried out by dividing the rats into the experimental group (5 rats) and the control group (5 rats). Oral administration of the composition of [Table 1] was conducted 30 minutes before swimming and oral administration of physiological saline was administered to the control group. The test results were compared with mean and mean error. Student's t test was used for comparison between the groups. Statistical significance was determined when the p-value was less than 5%.

(2) Change in exercise performance

The swimming time of the experimental group and the control group was compared and shown in Table 2 and FIG. 1 below.

Rat swimming time (sec. Control group Experimental group Average 279.00 449.00 Standard Deviation 10.95 18.17

As shown in [Table 2] and FIG. 2, in the experimental group administered with the composition according to the present invention, the swim time extension effect was statistically significant as compared with the control group. Thus, it can be seen that the composition according to the present invention can improve exercise performance by inducing fatigue or restoring stress.

(3) Changes in blood LDH

Immediately after the end of swimming, the blood was immediately collected and the blood was dispensed, and the blood was centrifuged at 4 ° C and 3000 rpm for 5 minutes and 15 minutes, respectively, to obtain serum, and blood LDH was measured. LDH was measured using a UV-Rate assay kit (Asan Pharm. Co.) With a Prime Automatic Clinical Chemistry Analyzer. The measurement results are shown in Table 3 below and FIG.

Changes in blood LDH (IU / L) Control group Experimental group Average 2399.00 1196.40 Standard Deviation 135.48 70.80

As shown in Table 3 and FIG. 2, the experimental group administered with the composition according to the present invention showed a statistically significant inhibitory effect on serum LDH elevation as compared with the right control group.

(4) Changes in blood lactate

Blood was collected immediately after the end of swimming, blood was smeared into sst-tube and Naf-tube, and blood was centrifuged at 4 ° C and 3000 rpm for 5 minutes and 15 minutes, respectively. Serum was separated and lactic acid was measured according to the enzymatic colorimetric method. The measurement results are shown in Table 4 and FIG. 3 below.

Changes in blood lactate (mg / dL) Control group Experimental group Average 30.36 14.36 Standard Deviation 2.09 1.21

The serum lactate content was statistically significantly lower in the experimental group administered with the composition of the present invention than in the control group as shown in Table 4 and FIG. 3. Considering that lactic acid is a fatigue substance accumulated on muscles when fatigue is accumulated, the composition according to the present invention is effective for reducing fat content or stress by restricting the content of lactic acid.

(5) Changes in blood SOD activity

Immediately after the end of swimming, blood was drawn immediately, and the blood was dispensed after each blood sampling. The blood was centrifuged at 4 ° C and 3000 rpm for 5 minutes and 15 minutes, respectively, to obtain serum, and the activity of blood SOD was measured. SOD is an enzyme that removes active oxygen, which acts to neutralize the active oxygen when it is in excess of the body, and when active oxygen is generated due to stress, it shows strong activity. To measure the activity of SOD in the blood, 0.1 ml of plasma of mouse, reagent A (pH 7.0, containing a final concentration of 0.2 mM hydroxylamine and 0.2 mM hippoxatin in aqueous solution, or a final concentration of 1.77 mM hydroxylamine O-sulphonic acid And 0.2 ml of a solution containing lyophilized powder under reduced pressure containing hippoxatin) and distilled water or KCN (final concentration 1 mM, 0.5 ml) were mixed. 0.2 ml of reagent B (final concentration of 1.25 mU / ml of XOD, 0.1 mM of EDTA-2Na, 20.8 mM of KH2PO4 and 15.6 mM of Na2B4O7) was added thereto and the mixture (1 ml) was left without stirring for 30 minutes at 37 ° C 2.0 ml of Reagent C (final concentration of 300 占 퐂 / ml sulfonic acid, 5 占 퐂 / ml N-1-naphthylethylenediamine, 16.7% acetic acid) was used as a coloring reagent. The final mixture thus treated was stored at room temperature for 20 minutes and the absorbance at 550 nm was measured to evaluate the activity of SOD. The measurement results are shown in Table 5 below and FIG.

 Serum SOD activity (NU / ml) Control group Experimental group Average 980.20 790.20 Standard Deviation 83.72 41.89

As shown in Table 5 and FIG. 4, serum SOD was statistically significantly lower in the experimental group administered with the composition of the present invention than the control group. Therefore, the composition according to the present invention is effective for suppressing stress by removing active oxygen due to stress.

2. Human experiment

(1) Blood lactate concentration measurement

end. Experimental Method

We conducted a human experiment on students aged 20-25 who were living in dormitories who were university students in Shinchon area and were diagnosed as chronic fatigue syndrome. The subjects were divided into five experimental groups (5 students) and control students (5 students) who had no experience of ingestion of medication or health care within one month and no specific disease. There was no significant difference between the groups.

Subject Control group Experimental group Average Standard Deviation Average Standard Deviation Age (yr) 23.40 1.82 23.40 1.52 Height (cm) 170.60 2.07 173.20 1.79 Weight (kg) 65.00 1.58 62.20 6.42 Body fat (%) 14.20 1.92 14.60 2.30

Exercise, drinking, smoking and overeating were prohibited for fatigue or stress induction for 24 hours, and they arrived at the laboratory around 10:00 am and started to stabilize. Treadmill exercise test was used as a stress inducer. In addition to subjective exercise intensity according to subject 's self - perception, the maximal exercise was induced by verbal reinforcement at the end of exercise, while observing dyspnea, facial flushing, gait irregularity, and facial expression. During the exercise test, the temperature of the laboratory was kept at 20-25 ℃ and the humidity was kept below 60%. In the experimental group, 1 bottle of the composition according to [Example 1] was taken in a stable state 30 minutes before the start of the exercise, and no information on the consumed beverage was provided to exclude the psychological effect as much as possible. In the control group, physiological saline was added to the same bottle. For the analysis of data on the test results, mean (M) and standard deviation (SD) of each measurement items were obtained using the Pharmacologic static package Ver 4.0 program and ANOVA test was performed. The statistical significance level was p <0.05 Respectively.

I. Blood lactate concentration measurement result

The concentration of lactate in the blood was measured at the time of stabilization immediately prior to the use of the composition, immediately after the exercise, 5 minutes after the exercise (5 minutes after the recovery), and after the exercise And 10 minutes after the exercise (10 minute recovery period). The measurement results were as shown in [Table 7] and FIG. 5 below.

Changes in Lactate Concentration in Human Blood (mM / L) ballast Immediately after exercise Recovery 5 minutes Recovery 10 minutes Control group Average 1.68 9.75 10.00 7.57 Standard Deviation 0.22 0.14 0.23 0.36 Experimental group Average 1.66 8.63 8.61 5.72 Standard Deviation 0.07 0.10 0.08 0.10

As shown in Table 7 and FIG. 6, the concentration of lactic acid in blood was significantly decreased in all the experimental groups taking the composition according to the present invention at 5 minutes and 10 minutes after the exercise and after the exercise, . Accordingly, the composition according to the present invention is expected to help relieve fatigue or stress by lowering the blood lactate concentration.

(2) Fatigue or stress recovery survey

end. Investigation method

We conducted a human experiment on students aged 20-25 who were living in dormitories who were university students in Shinchon area and were diagnosed as chronic fatigue syndrome. The subjects were divided into five experimental groups (5 students) and control students (5 students) who had no experience of ingestion of medicines or medical supplies and no specific diseases within 1 month. There was no significant difference between the groups.

Subject Control group Experimental group Average Standard Deviation Average Standard Deviation Age (yr) 23.80 1.78 23.80 1.64 Height (cm) 172.20 1.30 173.60 1.34 Weight (kg) 65.20 1.48 62.40 6.58 Body fat (%) 14.60 2.30 15.00 3.08

Exercise, drinking, smoking and overeating were prohibited for fatigue or stress induction for 24 hours, and they arrived at the laboratory around 10:00 am and started to stabilize. Treadmill exercise test was used as a stress inducer. In addition to subjective exercise intensity according to subject 's self - perception, the maximal exercise was induced by verbal reinforcement at the end of exercise, while observing dyspnea, facial flushing, gait irregularity, and facial expression. During the exercise test, the temperature of the laboratory was kept at 20-25 ℃ and the humidity was kept below 60%. In the experimental group, 1 bottle of the composition according to [Example 1] was taken in a stable state 30 minutes before the start of the exercise, and no information on the consumed beverage was provided to exclude the psychological effect as much as possible. In the control group, physiological saline was added to the same bottle. After the test, the questionnaire was evaluated 30 minutes after the end of the fatigue and stress-induced exercise test. The questionnaire was drawn at the scale of 5 scale (1-5) with the minimum value of 1 for each yield and the maximum value of 5 1 ------ 2 ------ 3 ------ 4 ------ 5: maximum, strong case, many cases).

I. Survey results

The evaluation of the questionnaire according to fatigue or restoration of stress was shown in [Table 9] and Figure 6 below.

Evaluation of the phenomenon of fatigue or stress caused by taking the composition Control group Experimental group Average Standard Deviation Average Standard Deviation Fever 2.40 0.50 1.05 0.50 headache 2.40 0.53 1.65 0.61 Fatigue after exercise 4.60 0.67 2.56 0.58 Muscle weakness 3.50 0.71 2.50 0.61 Nervousness 3.80 0.20 3.10 0.51 Incapacity 3.80 0.69 2.90 0.72 pain in the neck 3.70 0.71 2.45 0.56 Malaise 4.10 0.50 2.50 0.56 Decreased concentration 4.10 0.51 2.60 0.90 Joint pain 2.80 0.26 2.50 0.71

As shown in [Table 9] and Fig. 6, the experimental group showed that fatigue symptoms related to fatigue or stress such as fever, headache, fatigue after exercise, muscle weakness, nervous irritability, incapacity, discomfort, Compared with the control group. Therefore, the composition according to the present invention can be said to be effective for restoring fatigue or stress.

[Example 3] Fatigue or stress prevention effect

1. Animal experiments

(1) Experimental method

In a transparent plastic container of 25 × 40 × 17 cm, 15 cm of distilled water was filled in, and the swimming time was measured at 25 ± 1 ° C in the same time under the same conditions. To induce fatigue, 5% of the weight of the rats during swimming was fixed on the back of the neck of the rats. The end of swimming was settled for 5 seconds until the nose was submerged in the water, until the sinking occurred. At that time, the experiment was terminated. In the experiment, the rats were divided into the experimental group (5 rats) and the control group (5 rats), and the test group was orally administered the composition of Table 1 and the control group was orally administered with physiological saline. The composition and physiological saline were each administered for 5 days and were administered 30 minutes before the start of the stress-induced swimming. The test results were compared with mean and mean error. Student's t test was used for comparison between the groups. Statistical significance was determined when the p-value was less than 5%.

(2) Change in swimming time

The swimming time of the experimental group and the control group was measured and the results are shown in Table 10 and FIG.

Rat swimming time (sec.) By long-term administration of the composition Control group Experimental group Average 287.00 439.00 Standard Deviation 11.40 11.40

As shown in [Table 10] and Fig. 7, the swimming time extension effect was observed statistically in the experimental group as compared with the control group. Accordingly, the composition according to the present invention can improve exercise performance by preventing fatigue or stress.

(3) Changes in blood LDH

Immediately after the end of swimming, blood was sampled immediately, and blood was collected from each blood sample. After centrifugation at 4 ° C and 3000 rpm for 5 minutes and 15 minutes, serum was obtained and blood LDH was measured. LDH was measured using a UV-Rate assay kit (Asan Pharm. Co.) With a Prime Automatic Clinical Chemistry Analyzer. The measurement results are shown in Table 11 and FIG. 8 below.

Changes in blood LDH (IU / L) depending on long-term administration of the composition Control group Experimental group Average 2419.00 1155.20 Standard Deviation 152.17 109.56

As shown in Table 11 and FIG. 8, the long-term administration of the composition according to the present invention showed a statistically significant inhibition of serum LDH elevation as compared with the control group.

(4) Changes in blood lactate

Blood samples were collected immediately after swimming, and sst-tube and Naf-tube were dispensed respectively. After centrifugation at 4 ° C and 3000 rpm for 5 minutes and 15 minutes, serum was obtained and blood lactate was measured. Serum was separated and lactic acid was measured according to the enzymatic colorimetric method. The measurement results are shown in Table 12 and FIG. 9 below.

Changes in Blood Lactate (mg / dL) Control group Experimental group Average 31.00 12.22 Standard Deviation 2.75 0.98

As shown in Table 12 and FIG. 9, the composition according to the present invention was statistically significantly lower than that of the control group in the long-term administration group.

(5) Changes in blood SOD activity

After the end of swimming, the blood was immediately collected, and each blood was dispensed. After centrifuging at 4 ° C and 3000 rpm for 5 minutes and 15 minutes, serum was obtained and the degree of activity of blood SOD was measured. To measure the activity of SOD in the blood, 0.1 ml of plasma of mouse, reagent A (pH 7.0, containing a final concentration of 0.2 mM hydroxylamine and 0.2 mM hippoxatin in aqueous solution, or a final concentration of 1.77 mM hydroxylamine O-sulphonic acid And 0.2 ml of a solution containing lyophilized powder under reduced pressure containing hippoxatin) and distilled water or KCN (final concentration 1 mM, 0.5 ml) were mixed. 0.2 ml of reagent B (final concentration of 1.25 mU / ml of XOD, 0.1 mM of EDTA-2Na, 20.8 mM of KH2PO4 and 15.6 mM of Na2B4O7) was added thereto and the mixture (1 ml) was left without stirring for 30 minutes at 37 ° C 2.0 ml of Reagent C (final concentration of 300 占 퐂 / ml sulfonic acid, 5 占 퐂 / ml N-1-naphthylethylenediamine, 16.7% acetic acid) was used as a coloring reagent. The final mixture thus treated was stored at room temperature for 20 minutes and the absorbance at 550 nm was measured to evaluate the activity of SOD. The measurement results are shown in Table 13 and FIG. 10 below.

Serum SOD activity (NU / ml) Control group Experimental group Average 990.40 776.20 Standard Deviation 74.23 33.81

As shown in Table 13 and FIG. 10, serum SOD was significantly lower in the group administered with the composition for a long time than the control group.

2. Human experiment

(1) Blood lactate concentration measurement

end. Experimental Method

We conducted a human experiment on students aged 20-25 who were living in dormitories who were university students in Shinchon area and were diagnosed as chronic fatigue syndrome. The subjects were divided into experimental group (5 persons) and control group (5 persons) in five healthy subjects who had no experience of ingestion of medicines or alimentary drugs within 1 month, There was no significant difference between the groups.

Subject Control group Experimental group Average Standard Deviation Average Standard Deviation Age (yr) 24.00 1.41 24.20 0.84 Height (cm) 171.60 2.70 173.80 1.92 Weight (kg) 63.60 5.41 62.80 6.57 Body fat (%) 14.80 2.28 15.20 2.59

Exercise, drinking, smoking and overeating were prohibited for fatigue or stress induction for 24 hours, and they arrived at the laboratory around 10:00 am and started to stabilize. Treadmill exercise test was used as a stress inducer. In addition to subjective exercise intensity according to subject 's self - perception, the maximal exercise was induced by verbal reinforcement at the end of exercise, while observing dyspnea, facial flushing, gait irregularity, and facial expression. During the exercise test, the temperature of the laboratory was kept at 20-25 ℃ and the humidity was kept below 60%. In the case of the experimental group, one bottle of the composition according to [Example 1] was ingested for 5 days every day, and no information about the consumed beverage was provided to exclude the psychological effect to the maximum. In the control group, physiological saline was added to the same bottle. All experimental results showed mean and mean error. Student's t-test was used for comparison between the groups, and statistical significance was determined when the p value was less than 5% compared with the control group.

I. Blood lactate concentration measurement result

The lactic acid concentration in the blood was measured at the time of the stabilization (ballast) just before the composition was applied, immediately after exercise, 5 minutes after the exercise (5 minutes after the recovery), and the blood lactate concentration was measured by measuring the blood lactate concentration. ) And 10 minutes immediately after exercise (10 minutes recovery period). The measurement results were as shown in [Table 15] and FIG. 11 below.

Changes in Lactate Concentration in Human Blood (mM / L) ballast Immediately after exercise Recovery 5 minutes Recovery 10 minutes Control group Average 1.68 9.80 10.21 7.79 Standard Deviation 0.22 0.14 0.23 0.36 Experimental group Average 1.01 6.15 5.62 3.62 Standard Deviation 0.07 0.10 0.08 0.10

As shown in Table 15 and FIG. 11, the concentration of serum lactate was significantly lower in the control group than that of the control group in all the experimental groups in which the composition according to the present invention was used for a long period of time, At 5 and 10 minutes, the lactate concentration decreased significantly compared to the control. Therefore, this composition can be said to help prevent fatigue or stress by lowering the concentration of blood lactate depending on fatigue or stress.

(2) Fatigue or stress prevention survey

end. Investigation method

We conducted a human experiment on students aged 20-25 who were living in dormitories who were university students in Shinchon area and were diagnosed as chronic fatigue syndrome. The subjects were divided into 5 experimental groups (5 students) and control group (5 students) who had no experience of ingestion of medicines or preservatives and no specific diseases within 1 month. The subjects were as follows There was no significant difference between the groups.

 Subject Control group Experimental group Average Standard Deviation Average Standard Deviation Age (yr) 24.20 1.64 24.60 1.14 Height (cm) 172.20 2.58 174.20 1.30 Weight (kg) 64.40 3.64 636.00 6.22 Body fat (%) 15.60 0.89 16.00 1.87

Exercise, drinking, smoking and overeating were prohibited for fatigue or stress induction for 24 hours, and they arrived at the laboratory around 10:00 am and started to stabilize. Treadmill exercise test was used as a stress inducer. In addition to subjective exercise intensity according to subject 's self - perception, the maximal exercise was induced by verbal reinforcement at the end of exercise, while observing dyspnea, facial flushing, gait irregularity, and facial expression. During the exercise test, the temperature of the laboratory was kept at 20-25 ℃ and the humidity was kept below 60%. In the case of the experimental group, 1 bottle of the composition according to [Example 1] was ingested for 5 days each day, and no information about the consumed beverage was provided to exclude the psychological effect as much as possible. In the control group, physiological saline was added to the same bottle. After the test, the questionnaire was evaluated 30 minutes after the end of the fatigue and stress-induced exercise test. The questionnaire was drawn at the scale of 5 scale (1-5) with the minimum value of 1 for each yield and the maximum value of 5 1 ------ 2 ------ 3 ------ 4 ------ 5: maximum, strong case, many cases).

I. Survey results

The evaluation of the questionnaire according to fatigue or restoration of stress was shown as [Table 17] and Figure 12 below.

Evaluation of the phenomenon of fatigue or stress due to long-term use of the composition Control group Experimental group Average Standard Deviation Average Standard Deviation Fever 2.50 0.51 0.70 0.54 headache 2.50 0.52 1.60 0.56 Fatigue after exercise 4.80 0.68 2.30 0.61 Muscle weakness 4.00 0.75 2.40 0.68 Nervousness 4.10 0.21 3.00 0.45 Incapacity 3.90 0.78 2.80 0.68 pain in the neck 3.95 0.78 2.40 0.69 Malaise 4.50 0.55 2.10 0.75 Decreased concentration 4.30 0.55 2.10 0.80 Joint pain 2.90 0.28 2.00 0.69

As shown in Table 17 and FIG. 12, the test group in which the composition according to the present invention was ingested for a long time showed fatigue symptoms due to fatigue or stress such as fever, headache, fatigue after exercise, muscle weakness, nervous irritation, , Fatigue, loss of concentration, joint pain, etc. were significantly less than those of the control group. Accordingly, the composition according to the present invention can be said to be effective for prevention of fatigue or stress.

[Example 4] Toxicity experiment

Six - week old SD rats were used for acute toxicity tests. Five rabbits were separated from each other, and the composition according to Example 1 was dissolved and orally administered at a dose of 1 g / kg / ml for 5 days and observed for 9 days. After the administration of the composition, mortality, clinical symptoms, and weight changes of the animals were observed, and hematological tests and blood biochemical tests were carried out, and autopsy was performed to visually examine the abdominal organs and thoracic organs.

As a result of administration of the composition, there were no dead rats, and all rats were in good health during visual observation. After the administration of the composition, there was no significant clinical symptoms other than slight increase in body weight in all rats, and normal activities were normal. No specific findings were observed in blood tests or blood biochemical tests. After 9 days, all mice were dissected and examined for each tissue and organs. No abnormalities were found in all tissues and organs. Accordingly, the composition according to the present invention can be considered to be safe for human body.

Claims (4)

Angelica angustifolia, decaccin and / or decancinol angelate, or decaccin and / or decascinol angelate as active ingredients; Rice husk extract; And a non-waxy extract, as an active ingredient, for preventing or restoring fatigue or stress. The method of claim 1,
A composition for preventing or restoring fatigue or stress, further comprising a pharmaceutically acceptable carrier or a pharmaceutical additive, or a pharmaceutically acceptable food additive. 3. The method of claim 2,
Wherein the food additive is at least one selected from diastase, GABA, Glutathione, aspartic acid, methionine, taurine, fructose, vitamin C, vitamin B, catechin, mineral, citric acid, herb flavor, sodium citrate, Or a pharmaceutically acceptable salt thereof. 4. The method of claim 3,
Wherein the sweetener is brown sugar and / or enzyme-treated stevia.

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