KR20160023617A

KR20160023617A - Color therapy cosmetic composition and a method of manufacturing

Info

Publication number: KR20160023617A
Application number: KR1020150118108A
Authority: KR
Inventors: 김형길; 김윤희; 안혜정
Original assignee: 김형길
Priority date: 2014-08-21
Filing date: 2015-08-21
Publication date: 2016-03-03

Abstract

The present invention relates to a color therapy cosmetic composition containing an active component of a composite extract extracted from kelp, sea mustard, coffee berries, black beans, and cacao fruits, and to a manufacturing method thereof. The color therapy cosmetic composition of the present invention comprises a composite extract of from kelp, sea mustard, coffee berries, black beans, and cacao fruits. The present invention has the purpose of providing a color therapy cosmetic composition using a composite extract obtained by extracting black plants among natural plants having a skin improving effect such as reducing and preventing skin wrinkles, moisturizing, and reducing skin stimulation, and a method for manufacturing the same.

Description

TECHNICAL FIELD The present invention relates to a color therapy cosmetic composition and a method of manufacturing the same,

The present invention relates to a color therapy cosmetic composition and a manufacturing method thereof, and more particularly, to a color therapy cosmetic composition containing an active ingredient of a combined extract of kelp, sea bream, coffee bean, black bean and cacao fruit.

In general, harmful oxygen is a by-product generated during the process of making energy in the body, which is also referred to as active oxygen. Active oxygen is produced by the normal metabolism of oxygen and plays a major role in cell signaling and homeostasis.

However, due to environmental stress, the concentration of reactive oxygen species can increase dramatically and can play a major role in cell structure damage, which is oxidative stress. Too much active oxygen in the body damages cells and genes, promotes aging and causes various diseases directly or indirectly. Active oxygen has been mainly associated with cancer promoting processes and is known to promote cancer cell changes. However, recent research has shown that reactive oxygen species can alter genes and attack normal cell genes, causing inflammation of nearby cells, It is known to cause.

In addition, active oxygen acts as an immune function and signal transducer to eliminate germs and foreign bodies, but when it occurs, it attacks the cells and breaks down the immune system and causes many diseases. When UV rays are formed, melanin pigment is formed. As a result, when the metabolism is declined, spots and freckles are generated and active oxygen oxidizes the collagen constituting the skin. For this reason, collagen is weakened and the skin loses elasticity and wrinkles.

Therefore, active oxygen is also involved in inflammation reaction and immune system, and it is closely related to skin diseases as well as diseases, and thus efforts are being made to reduce active oxygen.

KR 10-0436384 B1 KR 10-0969121 B1

Disclosure of the Invention The present invention has been made in view of the above-mentioned problems, and it is an object of the present invention to provide a cosmetic composition for skin, which is obtained by extracting black plants from natural plants having skin improving effect such as moisturizing effect, The present invention provides a therapeutic cosmetic composition and a manufacturing method thereof.

In order to achieve this object effectively, the color therapy cosmetic composition of the present invention comprises a combined extract of kelp, seaweed, coffee fruit, black soybean and cacao fruit.

At this time, the complex extract is composed of 1 to 25 parts by weight of sea tangle, 1 to 25 parts by weight of sea mustard, 1 to 25 parts by weight of roasted coffee beans, 1 to 25 parts by weight of black soybean, and 1 to 25 parts by weight of roasted cacao fruit.

The combined extract may also contain at least one selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diether ether Can be extracted with one kind of solvent.

The combined extract is 0.1 to 10 parts by weight based on 100 parts by weight of the total cosmetic composition.

In the meantime, the method for producing a color therapy cosmetic composition of the present invention comprises 1 to 25 parts by weight of kelp, 1 to 25 parts by weight of sea mustard, 1 to 25 parts by weight of roasted coffee beans, 1 to 25 parts by weight of black beans, 1 to 25 parts by weight of a mixture of the first step and the first step to prepare a mixture of 5 to 10 times the weight of the solvent to the mixture to produce the second step and the second step, And a third step of concentration and drying.

Since the present invention includes a combined extract of kelp, sea bream, coffee, black bean and cacao, it has a wrinkle-reducing, moisturizing and irritation-reducing effect, so that the skin cosmetic composition of the present invention can be applied to the skin.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart showing a method of manufacturing a color therapy cosmetic composition according to an embodiment of the present invention; FIG.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the drawings, the same reference numerals are used to designate the same or similar components throughout the drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. In addition, the preferred embodiments of the present invention will be described below, but it is needless to say that the technical idea of the present invention is not limited thereto and can be variously modified by those skilled in the art.

1 is a flowchart showing a method of manufacturing a color therapy cosmetic composition according to an embodiment of the present invention.

As shown, the color therapy cosmetic composition of the present invention includes a combination extract of kelp, sea bream, coffee, black bean and cacao.

The kelp added to the compound extract contains many pigments such as carotenes, xanthophylls and chlorophyll, carnitides such as mannit and laminarin, which are made by carbon assimilation, and alginic acid, which is a component of the cell wall, and iodine, vitamin B2 , And glutamic acid.

The composition of seaweed is approximately 16% moisture, 7% protein, 1.5% fat, 49% carbohydrate and 26.5% inorganic salts, 20% of carbohydrates are cellulose, and the rest are polysaccharides such as alginate and laminarin. In particular, there are many inorganic salts such as iodine, potassium and calcium.

Laminin in the sea tangle amino acid has the effect of lowering the blood pressure, Fucose (Fucose) is an anti-cancer and antioxidant component of the body's tissues and cells to prevent aging.

The seaweed contains not only a high calcium content, but also a high absorption rate and a high content of iodine, which is a major component of the thyroid hormone. It also contains an amino acid called laminin, which acts as a hypotensive agent, and has an effect of reducing the amount of cholesterol in the blood. In addition, since the amount of fibrous material is high, the movement of the intestines is promoted, which is effective in preventing constipation which is likely to occur in pregnant women.

100g of dried wakame contains 15g of protein, 6.1g of sodium, 5.5g of potassium, 960mg of calcium, 1,800IU of vitamin A, 1.15mg of vitamin B2 and 15g of vitamin C and also contains water quality which lowers the blood cholesterol level.

Fucoxanthin, a pigment component of brown seaweed such as seaweed, kelp, has excellent antioxidant and anticancer effects. In addition, the skin moisturizing effect can be expected by the moisturizing ingredient of fucoidan.

Coffee beans are composed of polysaccharides, lipids, organic amino acids, proteins, minerals, and caffeine. Generally, the content of these components is 37 to 55% of polysaccharides, 11 to 13% of lipids, 11 to 16% of organic amino acids, and 4 to 5% of proteins, although it varies slightly depending on the kind of green beans (Arabica, Robusta, , 3 ~ 5% of minerals, 2% of fatty acids, 1% of chlorogenic acid, trigonelline, and caffeine respectively.

Roasted beans contain 1 to 2% of caffeine, 2 to 5% of chlorogenic acid, 5.0% of tannin, and free amino acids such as glutamic acid and aspartic acid.

Among them, chlorogenic acid is a polyphenol compound and has an antioxidant function.

In addition, caffeic acid contained in coffee is a powerful antioxidant and has anti-inflammatory effect.

Black beans are a variety of soybean varieties that are widely distributed in Korea, China, Japan, and Siberia. Soybean contains about 20% lipid and about 40% protein. Triglycerides and phospholipids are the most abundant in total lipids. In addition, the seed coat contains a component called anthocyanin which is black.

The anthocyanin contained in black beans has a detoxifying effect on skin, which helps to prevent inflammation and increase the activity of skin cells by solving contaminated and dirty skin poisons.

In addition, black beans are known to have a beneficial effect on eye health, such as helping to regain eye fatigue and preventing cataracts. They have been shown to prevent hypertension, improve liver function, and inhibit free radicals , Isoflavones are abundant to maintain proper humidity of skin and prevent skin irritation and inflammation.

The cacao is the name of Theobroma cacao L, which means "god food".

These cacao origins are thought to have originated from Central America to the north of South America and have been cultivated in many countries of Central America since long ago BC and treated as very valuable foods with corn, Is a representative representative food.

Cacao beans differ in their production area, but in addition to being rich in dietary fiber in common, they contain a considerable amount of polyphenols and the like. Polyphenol is a typical antioxidant that inhibits free radicals that cause cancer, atherosclerosis, and diabetes. It has an effect of improving wrinkles on the skin and has a very high enzyme inhibitory activity.

In order to utilize the useful components of these additives, the present invention mixes and extracts kelp, sea bream, roasted coffee beans, black beans and roasted cacao beans to prepare a complex extract.

At this time, the combined extract may be extracted by hot water extraction or a mixture of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diether ether And a solvent extraction method in which the solvent is extracted with at least one solvent selected from the group consisting of:

In addition, the complex extract may be prepared by mixing extracts obtained from kelp, seaweed, roasted coffee beans, black beans, and roasted cacao beverages.

That is, the combined extracts of kelp, seaweed, coffee, black bean and cacao can be used as a mixed extract of the respective extracts.

Hereinafter, a method for producing the color therapy cosmetic composition of the present invention will be described.

The method of manufacturing a color therapy cosmetic composition according to the present invention comprises 1 to 25 parts by weight of kelp, 1 to 25 parts by weight of sea mustard, 1 to 25 parts by weight of roasted coffee beans, 1 to 25 parts by weight of black beans, 1 to 25 parts by weight of roasted cocoa beans, 25 parts by weight were mixed and mixed to prepare a mixture. (First step)

Next, an extract is prepared by adding a solvent of 5 to 10 times the weight of the mixture prepared in the first step to the mixture. (Second step)

If the solvent is less than 5 times the weight of the mixture prepared in the first step, there is no effect of mixing the solvent properly. If the solvent is mixed more than 10 times, the specific gravity of the mixture is decreased due to excessive mixing.

The solvent used in step 2 may be selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether At least one selected solvent may be used.

Next, the extract prepared in the second step is concentrated, dried, and filtered to complete the complex extract. (Third step)

At this time, the combined extract should be checked whether the solid content is 0.3 ~ 1.0%.

And 0.1 to 10 parts by weight of the complex extract thus prepared to prepare a color therapy cosmetic composition. (Step 4)

Such a color therapy cosmetic composition of the present invention can be prepared into any formulation conventionally produced.

Hereinafter, the present invention will be described in more detail with reference to examples and test examples of the present invention. However, the present invention is not limited to these embodiments.

Example One : Preparation of Composite Extract

Dried sea tangle, seaweed, roasted coffee bean, black bean and roasted cacao fruit were each finely pulverized and mixed in the weight ratios shown in Table 1 below.

500 ml of 70% strength ethanol was added as a solvent to the mixture and the mixture was boiled in an extractor equipped with a cooling condenser at a temperature of 100 DEG C for 8 hours.

The extract was filtered through 300 mesh filter paper, left at room temperature for 3 days, and the precipitate was filtered twice with filter paper of Edbentek No. 5 and Watman GF / C 47 mm filter paper. The mixture was concentrated at a temperature of 50 ° C using a vacuum concentrator, and then dried using a spray dryer (manufactured by BUCHI) to obtain a composite extract.

turn Example 1 One Kelp 20g 2 Seaweed 20g 3 coffee 20g 4 Black beans 20g 5 cacao 20g

Example 2 , Example 3 : Preparation of lotion containing complex extract

Lotions containing the complex extract prepared in Example 1 were prepared according to the conventional methods with the compositions and contents shown in Table 2 below.

(weight%) Control Example Example 2 Example 3 The complex extract (Example 1) - 0.5 1.0 Wax 1.0 1.0 1.0 Polysorbate 60 1.5 1.5 1.5 Sorbitan sesquioleate 0.5 0.5 0.5 Liquid paraffin 10.0 10.0 10.0 Sorbitan stearate 0.5 0.5 0.5 Pro-type glycerin monostearate 0.5 0.5 0.5 Stearic acid 1.5 1.5 1.5 Glyceryl stearate / FG-400 stearate 1.0 1.0 1.0 Propylene glycol 3.0 3.0 3.0 Carboxy polymer 0.1 0.1 0.1 Triethanolamine 0.2 0.2 0.2 antiseptic a very small amount a very small amount a very small amount Purified water To 100 To 100 To 100

Test Example One : NBT method Antioxidant effect measurement experiment using

In order to confirm the antioxidant effect of the color composite extract prepared in Example 1, the antioxidative activity was measured using NBT method using other antioxidant, that is, BHT, as a comparative sample under laboratory conditions. In order to measure the antioxidative effect, the active oxygen produced by xanthine and xanthine oxidase was measured by the NBT method and the effect of the test substance on the removal of active oxygen, that is, the effect of active oxygen scavenging was evaluated. The active oxygen generated by xanthine and xanthine oxidase was reacted with Nitro Blue Tetrazolium (NBT), and the blue color produced thereby was measured at a wavelength of 560 nm to measure the active oxygen scavenging rate. The method of measuring the active oxygen scavenging rate is as follows. 2.4 ml of 0.05 M Na ₂ CO ₃ , 0.1 ml of a 3 mM xanthine solution, 0.1 ml of a 3 mM EDTA solution, 0.1 ml of a BSA solution and 0.1 ml of a 0.72 mM NBT solution were added to a vial (5 ml vial) and thereto was added DMSO 0.1 ml of the sample solution prepared at a concentration of 0.1 (wt / v)% is added and left at 25 for 10 minutes. Thereafter, 0.1 ml of xanthine oxidase solution is added, and the mixture is stirred rapidly and incubation is started for 25 to 20 minutes. Then, 0.1 ml of ₆ Mm CuCl ₂ solution is added to stop the reaction, and the absorbance St at 560 nm is measured. The blank test is carried out in the same manner as above using distilled water instead of the sample solution, and the absorbance Bt is measured. Again, the blank of the sample solution is measured by the same procedure using distilled water instead of the xanthine oxidase solution to determine the absorbance Bo. The results of the effect were calculated by the formula 1, and the results are shown in Table 3.

St: Absorbance at 560 nm after enzyme reaction of sample solution

Bt: Absorbance at 560 nm after enzyme reaction of blank test solution

So: the absorbance at 560 nm before the reaction in the absence of enzyme in the sample solution

Bo: absorbance at 560 nm before the reaction in the absence of enzyme in the blank test solution

Name of sample Treatment concentration (w / v%) Antioxidative effect(%) The complex extract (Example 1) 0.1 95 BHT (Butylated Hydroxyan Isole, DUKSAN KOREA) 0.1 87

As shown in Table 3, the antioxidant effect was confirmed in all of the samples tested at the concentration of 0.1 (wt / v)%. In particular, the compound extract of the present invention prepared in Example 1 showed an antioxidative effect superior to that of BHT.

Test Example 2 : Check wrinkle improvement effect

In order to confirm the effect of improving the skin wrinkle on the complex extract of the present invention showing excellent antioxidative effect in Test Example 1, evaluation was performed by using image analysis.

Five women in their 40s and five women in their 50s were allowed to use the lotion of Example 3 containing the complex extract of the present invention twice a day for four weeks in the morning and evening and the call of skin wrinkles was measured over time Year.

The three-dimensional measurement of the PRIMOS High Resolution is performed by the computer outputting a quantitative calculation of the degree of bending of the parallel projection stripes projected onto the skin according to the height difference of the skin surface.

The measurement value obtained by using the skin meter was calculated by using the equation (2), and the results are shown in Table 4.

Before use after 2 weeks After 4 weeks Improvement rate after 2 weeks (%) Improvement rate after 4 weeks (%)

30s

15.48 14.34 13.82 7.33 10.69 18.02 16.85 15.98 6.49 11.30 17.79 17.20 17.01 3.33 4.40 15.47 14.35 13.88 7.20 10.28 16.69 15.81 15.47 5.29 7.33

40s

25.30 24.24 23.82 4.20 5.85 21.56 20.16 19.49 6.47 9.57 24.52 23.76 23.55 3.12 3.97 23.74 22.60 22.28 4.80 6.14 24.65 23.93 23.19 2.91 5.94 Average 5.11 7.54

As shown in Table 4, after the lotion of Example 3 containing the complex extract of the present invention was used, the skin wrinkle measurement value using a skin measuring device was 5.11% after 2 weeks of use and 7.54 %, And it was confirmed that the effect of improving the wrinkles of the skin was observed.

Test Example 3 : Confirmation of mitigation effect of cytotoxicity by ultraviolet irradiation

Human-derived fibroblasts were cultured in 96-well plates at a concentration of 1 × 10 ⁵ cells / well, and 0.2 ml of IMDM medium containing 10% FBS was added and cultured in a CO ₂ incubator for 18 hours. After the sample solution dissolved in DMSO was treated, the amount of ultraviolet light of 2 MED was irradiated for 10 minutes and cultured for 24 hours. After that, MTT assay was performed to examine cell viability. For MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by incubation for 4 hours. The medium was removed and 0.5 ml of DMSO was added to the formazan, and the absorbance was measured at 570 nm using an ELISA. BHT was used as a control.

Name of sample Treatment concentration (w / v%) Cell survival rate (%) The complex extract (Example 1) 0.1 87.2 BHT (Butylated Hydroxyan Isole, DUKSAN KOREA) 0.1 80.7

As shown in Table 5, the cytotoxic relaxation effect was confirmed in all of the samples tested at the concentration of 0.1 (wt / v)%.

The combined extract prepared in Example 1 showed better cytotoxic effect than BHT.

Test Example 4 : Confirm skin soothing effect

The present invention relates to a cosmetic composition containing a complex extract according to the present invention and a cosmetic composition containing the cosmetic composition containing the complex extract according to the present invention, And the degree to which stimulation induced was induced was evaluated relative to the degree of sedation.

First, taping was repeated 30 times with a scotch tape on both sides of the underbody to cause skin erythema. Table 6 shows the results of applying the lotion containing the complex extract according to the present invention and the lotion not containing the extract according to the present invention to the area where erythema was induced, respectively, and evaluating the degree of skin soothing after 3 hours.

Name of sample Degree of skin irritation Left Right Example 2 One 0 Example 3 0 0 Control Example 2 2

(No response: 0, very mild erythema: 1, faint or mild erythema: 2, clear erythema but weak erythema: 4, distinct erythema: 5)

As can be seen in Table 6, the lotion compositions of Example 2 and Example 3 containing the compound extract of the present invention had better skin irritation mitigating effect than the control without the compound extract.

Among them, the lotion formulation of Example 3 containing 1 wt% of the combined extract of the present invention showed the best skin irritation mitigation effect.

It will be apparent to those skilled in the art that various modifications, substitutions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims. will be. Therefore, the embodiments disclosed in the present invention and the accompanying drawings are intended to illustrate and not to limit the technical spirit of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments and the accompanying drawings . The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

Claims

Kelp, seaweed, coffee fruit, black bean and cacao fruit.

The method according to claim 1,
The combined extract comprises color therapy consisting of 1 to 25 parts by weight of kelp, 1 to 25 parts by weight of seaweed, 1 to 25 parts by weight of roasted coffee beans, 1 to 25 parts by weight of black beans and 1 to 25 parts by weight of roasted cacao fruit, Cosmetic composition.

The method according to claim 1,
Wherein the combined extract is at least 1 selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diether ether Wherein the composition is extracted with a solvent of a species.

The method according to claim 1,
Wherein the complex extract is 0.1 to 10 parts by weight based on 100 parts by weight of the total cosmetic composition.

1 to 25 parts by weight of seaweed, 1 to 25 parts by weight of seaweed, 1 to 25 parts by weight of roasted coffee beans, 1 to 25 parts by weight of black beans, and 1 to 25 parts by weight of roasted cacao fruit are mixed to prepare a mixture Stage 1;
A second step of preparing an extract by adding a solvent having a weight of 5 to 10 times the weight of the mixture prepared in the first step to the mixture;
A third step of concentrating and drying the extract prepared in the second step; &Lt; / RTI > wherein the cosmetic composition comprises a colorant.