KR20160021086A - Pharbitis nil (l.) choisy extract-containing composition for preventing hair loss and promoting hair growth - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss and promoting hair growth comprising a flower extract of Pharbitis nil (L.) Choisy as an active ingredient. The composition according to the present invention is useful for prevention of hair loss and hair growth Excellent effect on promotion

Description

[0001] PHARBITIS NIL (L.) CHOISY EXTRACT-CONTAINING COMPOSITION FOR PREVENTING HAIR LOSS AND PROMOTING HAIR GROWTH [0002]

The present invention relates to a composition for preventing hair loss and promoting hair growth. More specifically, the present invention relates to a composition for preventing and treating hair loss, comprising a flower extract of Pharbitis nil (L.) Choisy ( Ipomoea nil (L.) Roth) And more particularly to a composition for promoting hair growth.

The hair of the human body has more than 1.3 million hair and the hair of 100 ~ 150 thousand hair. Each hair has a different cycle, and the anagen, the active growth phase, the apoptotic regression phase, telogen , and the resting phase). This cycle repeats over 3 to 6 years, resulting in an average of 50 to 100 hairs dropped on average per day. In general, hair loss refers to a state in which hair or hair is less or no longer normal due to any cause.

Today, hair loss is caused by internal factors such as male hormone action, mental stress in daily life, accumulation of lipid peroxidation in the scalp, and these factors are known to be associated with hair loss symptoms . Recently, the hair loss population of the female is increasing due to the increase of the stress due to the change of the eating habits, the social environment and the like as well as the male hair loss, and the population suffering from the abnormal symptoms of the scalp and the hair is increasing, It is getting lower.

Of the above factors, male hormone affects hair loss. Testosterone binds to 5 alpha-reductase enzyme in the body to produce dihydroxytestosterone (DHT) And this DHT binds to the androgen receptor of hair roots and has a dominant influence on inhibiting hair follicle production of hair follicles. Therefore, studies are currently under way to develop anti-hair-loss agents using inhibitors of 5α-reductase enzyme.

Minoxidil in the United States of America and trichosaccharide in Crinos Co. are used as the hair growth promoters currently marketed. However, there is a problem of lack of clear effect and side effects . Propecia (Finasteride), which was developed by Merck as a treatment for prostate enlargement, was introduced into the treatment of alopecia. It is known that 5-α-reductase, an enzyme that acts on male hormone testosterone metabolism in hair follicles, ), But they have to be taken continuously and have side effects such as sexual dysfunction, allergy and depression.

In addition, it is known that female hair loss occurs due to multiple causes, such as that it is known to be one of the causes of blood flow disorder in the scalp as the aging proceeds as the scalp rapidly stops its development than the skull, and therefore, And the like, is a short-sighted prescription.

Considering such a problem, studies on a composition for preventing hair loss or for promoting hair growth, which do not have toxicity and do not cause side effects even for long-term use, have been actively studied by containing an extract derived from a natural product as an effective ingredient. However, in the case of related products containing extracts derived from natural products, there were many cases in which the antioxidant activity was not sufficient to prevent hair loss, accelerate hair growth and improve hair.

The inventors of the present invention have made intensive studies on a composition which is excellent in hair loss prevention and hair growth promoting effect even when there is no side effect in long term use. As a result, when a specific compound is used in combination with a flower extract of morning glory plant as a main active ingredient, And the effect of promoting hair growth is remarkable. Thus, the present invention has been completed.

As an example of using such a morning glory plant extract as a hair product, Korean Patent Laid-Open Publication No. 2002-0050261 discloses a hair loss enhancer, which is one of 5α-reductase inhibitors together with a tyrosinase promoter such as a sancho extract, Preventing or wool hair. However, there is no document yet disclosing the use of flower extracts other than morning glory seeds for hair loss prevention and hair growth promotion.

It is an object of the present invention to provide a composition for preventing hair loss and promoting hair growth, which has little side effects even when used for a long period of time, and has excellent hair loss prevention and hair growth promoting effect.

In order to achieve the above object, the present invention provides a composition for preventing hair loss and promoting hair growth, which comprises a flower extract of Pharbitis nil (L.) Choisy as an active ingredient.

In the present invention, it is preferable that the morning flower of the morning glory flower is extracted with a mixed solvent of one or more kinds of lower alcohol having 1 to 4 carbon atoms.

The present invention also provides a composition for promoting hair loss prevention and hair growth, which further comprises a blood flow promoter, hyaluronic acid, and lecithin in the flower extract of morning glory flower.

In the present invention, it is preferable that the morning flower of the morning glory flower is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition.

In the present invention, 0.0001 to 2% by weight of the blood flow promoter is added to the total weight of the composition; 0.1 to 10% by weight of hyaluronic acid; And lecithin in an amount of 0.001 to 10% by weight.

In the present invention, it is preferable that the blood flow promoting agent is at least one selected from the group consisting of nitrogen monoxide, glutathione, S-nitrosoglutathione, and S-nitrosoxystane.

The hair loss prevention and hair growth promoting composition of the present invention can be applied to hair selected from the group including hair, eyebrow, eyelash, conspiracy, armpit hair, chest hair, nose hair, and leg hair.

The composition for preventing hair loss and promoting hair growth of the present invention may have a form selected from a solution, a cream, a lotion, a shampoo, a hair tonic, a spray, and a gel.

Hereinafter, the configuration of the present invention will be described in more detail.

The present invention relates to a composition for preventing hair loss and for promoting hair growth comprising a flower extract of morning glory as an active ingredient.

The morning glory is an annual plant, which means Pharbitis nil (L.) Choisy ( Ipomoea nil (L.) Roth) of Convolvulaceae. Morning glory is originated in tropical Asia, including India, and about 256 varieties are known. The flowers are bloomed in various colors such as blue purple, red purple, white, and red in July ~ August, and one to three flowers hang on the peduncle from the axilla. Calyx is deeply divided into 5 pieces, the split pieces are thin and long, pointed end, and long hairs on back side. Corolla is 10-13cm in diameter and looks like a funnel. The bud is curled to the right in the shape of a brush. There are 5 stamens and 1 pistil. The fruit is in a calyx and is a round cape divided into three chambers. Each of the three boxes contains two seeds. The seeds of morning glory are used as medicines for Pharbitidis Semen. The seeds of green or red morning glory are called black ㅇ, and the seeds of white morning glory are called white 丑. It is a bit smelly, irritating, tastes worn, cold and poisonous. It is used when the body is swollen and has a strong diuretic effect and a diuretic effect and the hypochlorous action and chronic pyelonephritis and cirrhosis when the body is poured.

In the present invention, the flower part of the morning glory plant, that is, the flower part composed of the corolla (petal), the pistil, the stamen, the stamen and the calyx is used and the flower is taken and dried as it is or as necessary, Solvent extraction to obtain an extract.

The solvent to be used herein is not particularly limited and can be selected from commonly used solvents for extracting the components contained in the plant from the plant. For example, hot water or water; Lower alcohols such as methanol, ethanol, isopropyl alcohol and n-butanol; Polyhydric alcohols such as propylene glycol and 1,3-butylene glycol; Hydrocarbons such as n-hexane and toluene. In the present invention, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, isopropyl alcohol and n-butanol is used as an extraction solvent . When these lower alcohols are used as the extraction solvent, the extract can be directly incorporated into the composition of the present invention, but the extraction solvent can be once removed by distillation, dried and then mixed as required.

The content of the morning flower of the morning glory flower is preferably 0.001 to 10% by weight, more preferably 0.001 to 5% by weight based on the solid content of the flower extract. If the content of the flower extract of morning glory flower is less than 0.001% by weight, the effect of preventing hair loss and promoting hair growth can not be sufficiently exhibited. If the content is more than 10% by weight, it is difficult to maintain the formulation.

In addition, the composition of the present invention may contain a blood flow promoter, hyaluronic acid, and lecithin in addition to the flower morning flower of the morning glory.

As the blood flow promoter, it is preferable to use at least one selected from the group consisting of nitrogen monoxide, glutathione, S-nitrosoglutathion, and S-nitrosocysteine.

Nitric oxide (NO) is synthesized by NOS (Nitric Oxide Synthase) I, NOSII, and NOS III in the human body. Its detailed regulation mechanism is binding to glutathione Or cysteine (cysteine), which is one of the amino acids, to help relax the blood vessels. Therefore, the hair growth can be promoted through the blood flow improving action, and the flower extract of the morning glory flower, which is an effective ingredient, can be easily delivered into tissue cells targeted for hair loss prevention or hair growth promotion.

In the present invention, it has been unexpectedly found that hair loss prevention and hair growth promoting effects are unexpectedly improved by using any one or more of the substances capable of supplying nitrogen monoxide as the blood flow promoting agent together with flowerpox extract of morning glory .

The blood flow promoting agent is preferably used in an amount of 0.0001 to 2.0% by weight, more preferably 0.0001 to 1.5% by weight, based on the total weight of the composition. If the blood flow promoting agent is less than 0.0001% by weight, it is difficult to realize a sufficient blood flow effect. If the blood flow promoting agent is more than 2.0% by weight, skin irritation may occur.

In addition, the composition of the present invention further comprises lecithin or a hydrolysed lecithin thereof, whereby the effect of controlling intracellular blood flow, preventing oxidation, and enhancing cell delivery ability of the active ingredient by liposome formation, And hair growth promoting effect can be maximized.

The lecithin used in the present invention is prepared by extracting from egg yolk or soybean and then purified. The lecithin used in the present invention is a phosphatidylcholine, a phosphatidylserine, a phosphatidylethanolamine, a phosphatidylglycol, a phosphatidic acid, acid and hydrogenated phosphatidylcholine, and the hydrolyzate of lecithin refers to choline, phosphoric acid, glycerol, and fatty acids produced by hydrolysis of lecithin.

The lecithin or the hydrolysed lecithin preferably contains 0.001 to 10% by weight, more preferably 0.001 to 5% by weight, of the total weight of the composition.

In addition, in the present invention, by using hyaluronic acid (HA) in combination with a flower extract of morning glory flower and a blood flow improvement promoter, a drug delivery system (DDS) In addition to the function of preventing oxidization, the flower extract of morning glory and the blood flow improvement promoter are smoothly supplied to the scalp tissue cells, thereby maximizing the effect of preventing hair loss and promoting hair growth.

The hyaluronic acid is preferably used in an amount of 0.1 to 10% by weight, more preferably 0.5 to 5% by weight, based on the total weight of the composition.

Examples of the hair to which the composition of the present invention is applied include head hair, eyebrow hair, eyelashes, eyelashes, pubic hair, underarm hair, chest hair, nose hair, Various hairs may be included.

According to a preferred embodiment of the present invention, the composition of the present invention may be provided as a pharmaceutical composition or a cosmetic composition.

When the composition of the present invention is manufactured from a pharmaceutical composition, the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier. Such pharmaceutically acceptable carriers are those conventionally used in the field of manufacture and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, But are not limited to, polyvinylpyrrolidone, cellulose, purified water, syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. The pharmaceutical composition of the present invention may further contain additives commonly used such as lubricants, wetting agents, sweetening agents, flavoring agents, emulsifying agents, suspending agents, preservatives and the like in addition to the above components. The pharmaceutical composition of the present invention can be administered orally or parenterally, and is preferably administered by parenteral administration, more preferably by topical administration by application.

The appropriate dosage of the pharmaceutical composition of the present invention may vary depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate, .

The dose of the flower extract of morning glory which is an active ingredient contained in the composition of the present invention is 0.001 to 100 mg / kg, preferably 0.1 to 100 mg / kg, more preferably 1 to 50 mg / kg on an adult basis, May be administered once or several times a day.

The pharmaceutical composition of the present invention may be formulated as a solution, suspension, syrup or emulsion, excipient, powder or powder in an oil or an aqueous medium according to a method which can be easily carried out by a person having ordinary skill in the art to which the present invention belongs Granules, tablets or capsules, and may additionally contain a dispersant or stabilizer.

When the composition of the present invention is made of a hair cosmetic composition, the composition of the present invention includes not only the flower extract of morning glory, the blood flow improvement promoter, hyaluronic acid, and lecithin, which are effective ingredients thereof, but also components commonly used in cosmetic compositions, Such as antioxidants, stabilizers, solubilizers, vitamins, customary adjuvants such as pigments and flavoring agents, and carriers. The composition of the present invention can be used in combination with a hair growth inhibitor or an antiloading agent which has been conventionally used as long as it does not impair the hair loss prevention and hair growth promoting action by the active ingredient of the present invention.

As the carrier, it is possible to use purified water, monohydric alcohols (ethanol or propyl alcohol), polyhydric alcohols (glycerol, 1,3-butylene glycol or propylene glycol), higher fatty acids (palmitic acid or linolenic acid) But are not limited to, camellia oil, jojoba oil, olive oil, squalane, sunflower oil, macadamia peanut oil, avocado oil, or fatty acid glyceride). If necessary, surfactants, moisturizers, preservatives, antioxidants and the like may be added.

Examples of the surfactant that can be used in the cosmetic composition of the present invention include anionic surfactants such as alkylbenzenesulfonates, polyoxyalkylene alkylsulfate ester salts, alkylsulfate ester salts, olefin sulfonates, alkyl phosphates, polyoxyalkylene Alkyl ether phosphates, dialkyl sulfosuccinates, fatty acid salts, and the like. Nonionic surfactants include polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyhydric alcohol fatty acid partial esters, polyoxyethylene polyhydric alcohol fatty acid moieties Esters, polyglycerin fatty acid esters, polyoxyethylene hydrogenated castor oil derivatives, and fatty acid diethanolamides. Examples of the cationic surfactant include tertiary aliphatic amine salts, alkyltrimethylammonium halides and dialkyldimethylammonium halides. Examples of the amphoteric surfactants include amide betaine, imidazolinium betaine, sulfobetaine, And the like.

Examples of the moisturizing agent include glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, and sorbitol.

Examples of the preservative include benzoic acid, dehydroacetic acid, paraoxybenzoic acid esters (such as methyl parahydroxybenzoate, butyl parahydroxybenzoate), and phenoxyethanol.

Examples of the antioxidant include ascorbic acid and BHA. In addition, an ultraviolet absorber, an anti-inflammatory agent, and a refreshing agent may be added.

The formulation of the composition of the present invention may be any formulations that can be combined with the active ingredient, and examples of the form of hair loss prevention or hair growth include hair tonic, hair cream, hair lotion, hair shampoo, hair conditioner, hair conditioner, hair spray, Such as, but not limited to, solutions, sols gels, emulsions, oils, waxes, aerosols, and the like, such as powders,

The composition of the present invention has an excellent effect in preventing hair loss and promoting hair growth by containing a flower extract of morning glory as an active ingredient.

In addition, the composition of the present invention shows excellent effects on preventing hair loss and promoting hair growth and scalp by combining a flower flowering extract of morning glory flower, a blood flow promoter, hyaluronic acid, and lecithin. Did not induce.

1 to 3 show the compositions (1a, 1b and 1c) of Example 1 of the present invention, the purified water (2a, 2b and 2c) of Comparative Example 1 as a control group, 3c) were applied to the epilated skin of C57BL / 6 mice and photographed with a digital camera on day 0, day 10, and day 20, respectively.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

Production Example 1: Preparation of flower extract of morning glory

200 g of a flower of a morning glory plant ( Pharbitis nil (L.) Choisy) was washed with water and dried to obtain a powder. The powder was then added to 1000 ml of a 70% aqueous ethanol solution, maintained at 4 to 35 ° C for 1 to 6 hours, The extract was concentrated under reduced pressure. The concentrated sample was dried using a freeze dryer to obtain 4.2 g (2.1% yield) morning glory flower extract.

Production Example 2: Preparation of seed extract of morning glory (strawberry extract)

200g of seeds of morning glory plant ( Pharbitis nil (L.) Choisy) were dried and put into 1000ml of 70% ethanol solution, kept at 4 ~ 35 ℃ for 1 ~ 6 hours, filtered and the filtered extract And concentrated under reduced pressure. The concentrated sample was dried using a freeze dryer, and 4 g (2% yield) of morning glory seed extract was obtained.

Production Example 3: Example 1 and Comparative Examples 1 to 3

The compositions of Example 1 and Comparative Example 3 were prepared according to the compositions shown in Table 1 below. In addition, purified water was prepared as Comparative Example 1, and 5% solution of minoxynil (Hyundai Pharmaceutical, Minoxidil 50 mg / mL) was prepared as Comparative Example 2.

Experimental Example 1: Promoting hair growth promoting effect using mouse

1) Preparation of experimental animals and application of composition

A 5-week-old male C57BL / 6 mouse (Coatec Co., Ltd.) was housed in a feeding room where temperature (21 to 23 ° C), humidity (40 to 60% And then used in the experiment through a ballast.

That is, C57BL / 6 mice at 6 weeks of age were diluted 8 times with ethyl ether or Zolethyl 50 with physiological saline, and anesthetized by intraperitoneal injection of 0.2 mL with a 1 mL syringe. Then, an animal clipper (JP-6751) Joas dog hair clipper), and the hair was completely removed using a depilator (Nitrin cream, Ildong Pharma) and cleaned thoroughly.

The mice were divided into 7 mice per each group and sprayed on the hair removal portions of 0.25 mL each of the compositions of Example 1 and Comparative Examples 1 and 2. The mice were sprayed evenly at 10 o'clock every morning for 3 weeks Respectively.

2) Observation of hair growth effect in experimental animals

In order to confirm the hair growth state of the mice to which the composition of Example 1 and Comparative Examples 1 and 2 were applied, the hairs were photographed using a digital camera with visual observation on the 0th day, the 10th day, and the 20th day of the experiment .

As a result, in the experimental group to which the composition of Example 1 was applied, on the 10th day of application, the whole skin of the epilated skin appeared gray, and the hair growth phenomenon where the hair was relatively grown was sparsely black appeared in all 7 animals (See FIG. 1B). On the 20th day after application, it was confirmed that hair was grown close to the state before hair removal (see Fig. 1C).

On the other hand, in the experimental group to which the purified water of Comparative Example 1 was applied as the control group, hair growth was observed on the 10th day after application (see FIG. 2B), but hair growth was not progressed. There was a difference. On the 20th day after application, the skin appeared to be gray and partially black hair was short grown (Fig. 2C). In addition, in the mouse of the experimental group to which the minoxil of Comparative Example 2 was applied, a hair growth phenomenon in which the entire skin of the epilated skin appeared gray appeared on the 10th day (see Fig. 3b) The degree was slightly longer than that of Comparative Example 1, and was much shorter than that of Example 1 (see FIG. 3C).

On the 20th day after the application, hair of each mouse newly grown in the hair removal area was collected with an animal hair clipper (JP-6751), and the weight thereof was measured. The degree of recovery in comparison with the hair weight immediately before hair removal The results were as follows. The results were as follows.

As a result of the above-mentioned visual observation, it was confirmed that the composition of the present invention exhibits a hair growth promoting effect which is far superior to that of minoxynil used in conventional hair loss.

Experimental Example 2: Estimation of hair loss prevention effect and hair growth promoting effect through clinical experiment

1) Clinical test method

In order to confirm the hair growth effect of the composition of Example 1 and Comparative Examples 2 and 3, clinical subjects were selected by subjects having alopecia. That is, a total of 30 adult male and female 30 to 50 year olds with alopecia were divided into a group of 5 males and 5 females and applied 2 ml per day to the depilation area for 4 months, Respectively.

2) Clinical results

The evaluation of hair loss prevention and hair removal effect was made by evaluating the daily average amount of hair loss, glance determination, and improvement of subjective symptoms at the time of shaving in 5 stages (see Table 3) , And the score was given as a score of 5 points. The results of clinical trials are shown in Table 3.

As can be seen from the above Table 4, the composition of the present invention exhibited the hair loss improving effect as a whole in comparison with Comparative Example 2 and Comparative Example 3 in terms of visual determination, subjective symptoms, and hair loss at the time of hair loss. Comparing the results with the total judgment scores, it was confirmed that Example 1, which is the composition of the present invention, has an improvement of about 175-200% on average compared with the composition of Comparative Example 2 or Comparative Example 3.

In addition, during the clinical experiment period, no adverse effects were found in all the subjects using the composition of Example 1, and it was found that the composition was safe for human body.

Experimental Example 3: Skin irritation test

1) Test method

Twenty healthy men and women were subjected to skin stimulation tests using patches. 10% SDS (Sodium Dodecyl Sulfate) solution was used as a positive control, 10% SDS and the composition of Example 1 were applied to the patch at a ratio of 1: 1, adhered to the abdomen of the subject, and the degree of stimulation after 24 hours was determined . Distilled water was used as negative control group.

<Irritability determination>

3: Strong inflammatory response

2: erythema and slight inflammation

1: slight erythema

0: No change

2) Test result

When the composition of Example 1 was mixed with 10% SDS at a ratio of 1: 1, the irritation index was found to be almost 0.30, whereas the irritation index average in the positive control group 10% SDS was 2.65.

Formulation Example 1: Preparation of ointment for external use

Skin external ointment was prepared according to a conventional method with the composition shown in Table 6 below.

Formulation Example 2: Preparation of shampoo

Shampoos were prepared according to the usual methods with the compositions shown in Table 7 below.

Formulation Example 3: Production of hair tonic

doing A hair tonic was prepared by the usual method with the composition shown in Table 8.

Claims (7)

A composition for preventing hair loss and promoting hair growth, comprising a flower extract of Pharbitis nil (L.) Choisy as an active ingredient. The method according to claim 1,
Wherein the morningflower flower extract is obtained by extracting flowers of morning glory flower with a mixed solvent of one or more of lower alcohols having 1 to 4 carbon atoms. The method according to claim 1,
The composition for preventing hair loss and promoting hair growth,
Blood flow promoters;
Hyaluronic acid; And
lecithin;
The composition for preventing hair loss and promoting hair growth according to claim 1, The method of claim 3,
The composition for preventing hair loss and promoting hair growth may comprise,
0.001 to 10% by weight of a morning flower of a flower extract as a solid component;
0.0001 to 2% by weight of a blood flow promoter;
0.1 to 10% by weight of hyaluronic acid; And
0.001 to 10% by weight of lecithin;
Wherein the composition for preventing hair loss and for promoting hair growth is characterized by comprising The method according to claim 3 or 4,
Wherein the blood flow promoting agent is at least one selected from the group consisting of nitrogen monoxide, glutathione, S-nitrosoglutathione, and S-nitrosoxystane. The method according to one of claims 1 to 4,
The composition for preventing hair loss and promoting hair growth,
Wherein the composition is applied to hair selected from the group consisting of hair, eyebrows, eyelashes, intrigue, underarm hair, chest hair, nose hair, and leg hair. 5. The method according to any one of claims 1 to 4,
Wherein the composition for preventing hair loss and promoting hair growth has a form selected from a solution, a cream, a lotion, a shampoo, a hair tonic, a spray, and a gel.

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