KR20160020196A - Novel rice stripe with high productivity of rice seed and Process for preparing the same - Google Patents

Abstract

The present invention relates to a method for enhancing productivity of rice seeds, which comprises a step of suppressing expression of LOC_Os08g12430 genes present in genome DNA of rice, a method for producing rice seeds in which expression of LOC_Os08g12430 genes is suppressed, and rice seeds prepared thereby, which has enhanced seed productivity. According to a method for enhancing productivity of rice seeds by suppressing expression of LOC_Os08g12430 genes provided by the present invention, productivity of rice seeds can be enhanced without introducing foreign genes, thereby ruling out a risk likely to be caused by introduction of foreign genes to be widely utilized in mass-producing safer rice seeds.

Description

[0001] The present invention relates to rice varieties having increased seed productivity and a method for preparing the same,

The present invention relates to a rice cultivar having increased seed productivity and a method for producing the rice cultivar. More particularly, the present invention relates to a method for enhancing the yield of rice seeds comprising the step of inhibiting the expression of LOC_Os08g12430 gene present in genomic DNA of rice , LOC_Os08g12430 gene expression inhibited rice seeds, and rice seeds produced by the above method and having increased seed yield.

Rice (Oryza sativa), together with barley and corn, is one of the most important food crops in the world. The demand for rice is increasing due to population growth, lack of agricultural land due to industrialization, and climate change. Accordingly, various kinds of varieties are being developed. In recent years, there has been a development of a variety using a method for introducing a useful gene rather than a traditional breeding method. The search for such a useful gene and the development of a new variety using this gene have not only solved the food problem, but also affected the food industry as a whole It is being considered.

Rice is one of the most important crops in the world for the purpose of producing seeds. As a whole gene sequence has been revealed in recent years, a lot of useful genes have been secured and studies for preoccupying the technology using the useful genes have been actively carried out. In particular, it is expected that the use of genes involved in regulating the characteristics of rice, such as increasing the number of rice, increasing the kidney of rice, and controlling the flowering time of rice, is expected to ultimately achieve the purpose of increasing the yield of rice. Related researches are actively proceeding. For example, Japanese Patent Application Laid-Open No. 2006-248898 discloses a method for promoting the growth of crops and enhancing the yield by using a crop growth promoter containing the acetic acid fungus disruption product and the promoter, Japanese Patent No. 4462566 discloses a method for producing transgenic rice having an increased number of transgenic plants by introducing a specific gene into rice and transgenic rice produced by the above method and having an increased number of transgenic plants. Korean Laid-Open Patent Application No. 2014-0050122 discloses a transgenic rice plant having LOC_Os02g05840 A method of producing transgenic rice having improved seed yield by introducing a gene into rice and a transgenic rice produced by the above method and having improved seed yield have been disclosed. However, among the genes of rice that have so far been found, its function has been clarified very little, and from the viewpoint of ordinary biological functions, it is expected that a large number of genes involved in an increase in the yield of rice are expected, It is expected that the gene involved in the increase of rice production can be identified through the present study. However, only few genes have been identified that are involved in the increase of rice production.

Under these circumstances, the inventors of the present invention have sought to elucidate the genes involved in the increase of rice production, and as a result, they have found that the LOC_Os08g12430 gene is a gene involved in the regulation of the number of rice, the elongation length and flowering time of rice, It is possible to increase the production amount of rice, and the present invention has been completed.

It is an object of the present invention to provide a method for inhibiting the expression of the LOC_Os08g12430 gene to increase the yield of rice seeds.

Another object of the present invention is to provide a method for producing rice seeds in which the expression of LOC_Os08g12430 gene is suppressed.

Another object of the present invention is to provide rice seeds produced by the above method and having increased seed yield.

The present inventors randomly mutated T-DAN into rice genomic DNA in order to identify genes involved in the increase of rice yield, and selected the varieties whose flowering time was slower. As a result of analyzing the genomic DNA of the selected breed, it was confirmed that the LOC_Os08g12430 gene was inactivated by insertion of T-DNA into the LOC_Os08g12430 gene, and the phenotype of the LOC_Os08g12430 gene was analyzed. As a result, The length of the kidney was increased, the size of the cells was decreased, the stem thickness was increased, the number and length of the ears were increased, and the seed yield was increased.

Thus, it was found that the production of rice could be increased by inactivating the LOC_Os08g12430 gene present in the genomic DNA of rice.

In one embodiment of the present invention, the present invention provides a method for increasing the yield of rice seeds comprising the step of inhibiting the expression of the LOC_Os08g12430 gene present in genomic DNA of rice.

The term "LOC_Os08g12430 gene" of the present invention means a gene existing on the chromosome 8 of rice, its total size is 9252 bp, the coding region (LOC_Os08g12430.1) is 1893 bp, It is composed of two introns and can express a protein containing 631 amino acids. The expressed protein is composed of a plant homeo domain (PHD finger domain), a fibronectin type III domain (FNIII), and a VIN3 interacting domain (VID). Although the nucleotide sequence of the LOC_Os08g12430 gene is known, its function has not been known until now. The inventors of the present invention for the first time confirmed that the LOC_Os08g12430 gene was associated with an increase in yield through regulation of flowering time, regulation of kidney and regulation of stem thickness of rice.

In the present invention, the base sequence of the LOC_Os08g12430 gene is not particularly limited, but may preferably have the nucleotide sequence of SEQ ID NO: 1.

Inhibition of the LOC_Os08g12430 gene expression can be performed by inactivating the LOC_Os08g12430 gene inherently present in rice genomic DNA at DNA level, mRNA level and protein level. Due to the nature of rice seeds, And at the mRNA level. For example, as a method of inactivating at the DNA level, a conventional mutagenesis method can be used. One or more nucleotides constituting the LOC_Os08g12430 gene, which is inherently present in genomic DNA of rice, is substituted or deleted with another nucleotide , A method of inserting another polynucleotide into the polynucleotide constituting the LOC_Os08g12430 gene or a method of frame shifting one or more nucleotides constituting the LOC_Os08g12430 gene so that the normal LOC_Os08g12430 gene is not expressed can be used. In addition, a method of inactivating the LOC_Os08g12430 gene or a method of inactivating the mRNA transcribed from the LOC_Os08g12430 gene using siRNA or shRNA may be used as a method of inactivating the RNA at the RNA level . A specific method for inactivating the LOC_Os08g12430 gene at the DNA level and the mRNA level can be used without limitation as mutation methods commonly used in the art. For example, a homologous recombination method using a recombinant vector, a single cross-over recombination method, a T-DNA insertion method, or the like can be used.

In the present invention, the LOC_Os08g12430 gene is inactivated at the DNA level by generating a gene (SEQ ID NO: 5) in which the T-DNA is inserted into the Loss of the LOC_Os08g12430 gene using the T-DNA insertion method to inhibit the expression of the LOC_Os08g12430 gene .

As another embodiment for achieving the above object, the present invention provides a method for producing rice seeds with increased seed production, which comprises the step of inhibiting the expression of the LOC_Os08g12430 gene present in genomic DNA of rice.

As a specific example of the method of producing rice seeds in which the expression of LOC_Os08g12430 gene provided by the present invention is suppressed and the yield of seeds is increased, (a) T-DNA is inserted into the wild type LOC_Os08g12430 gene and the nucleotide sequence of SEQ ID NO: Synthesizing the mutated LOC_Os08g12430 gene and obtaining a recombinant vector comprising the mutated LOC_Os08g12430 gene; (b) introducing the obtained recombinant vector into a microorganism belonging to the genus Agrobacterium to obtain a transformant; (c) inoculating the obtained transformant into calli of rice to obtain a transformed callus in which the mutated LOC_Os08g12430 gene is introduced into the genomic DNA of rice; (d) regenerating the obtained transformed callus to obtain regenerated plant; And (e) cultivating the resulting regenerated plant, and harvesting the seeds therefrom.

In step (a), the mutated LOC_Os08g12430 gene refers to a gene in which the function of the LOC_Os08g12430 gene is inactivated by a conventional mutation. Generally, a functionally inactivated gene refers to a gene that does not function normally due to various causes. In the case of a gene encoding a protein, expression of the gene is suppressed, and a corresponding It may be a mutated gene which causes a phenomenon in which a protein is not produced or a production level is remarkably reduced or an abnormal protein is expressed.

In the present invention, the mutated LOC_Os08g12430 gene may be obtained by replacing part or all of the nucleotide sequences constituting the LOC_Os08g12430 gene with other nucleotide sequences, deleting part or all of the nucleotide sequences constituting the LOC_Os08g12430 gene or constructing the LOC_Os08g12430 gene Or a gene into which another base sequence is introduced into the base sequence. For example, in the present invention, a polynucleotide having the nucleotide sequence of SEQ ID NO: 5 in which T-DNA has been inserted in the LOS region of the LOC_Os08g12430 gene was used as the mutated LOC_Os08g12430 gene.

In addition, the recombinant vector may be a vector containing the mutated LOC_Os08g12430 gene. The vector used herein is not particularly limited, but is preferably a microorganism belonging to the genus Agrobacterium or a plant cell capable of maintaining its original function Shuttle vector, more preferably a gene capable of inserting the mutated LOC_Os08g12430 gene into genomic DNA of rice through a method such as homologous recombination after introduction into rice seed through an Agrobacterium sp. Microorganism , And most preferably a pGA3426 vector.

The recombinant vector may include a promoter capable of expressing the mutated LOC_Os08g12430 gene. The promoter is not particularly limited as long as it can exert its function in a plant cell. Preferably, the promoter is a promoter for overexpression, An inducible promoter expressed under stress conditions, a promoter for causing the target gene to be expressed at a certain time during plant growth, a promoter for expressing only a specific part of plant tissue, and an 'enhancer sequence enhancer sequences' combined with a promoter.

In addition, the LOC_Os08g12430 gene contained in the recombinant vector may be inserted directly into the polyclonal region of the vector or may be inserted into the polyclonal region together with the linker nucleotide sequence. The linker nucleotide sequence may be any of those commonly used in the art It can be a sequence.

In the step (b), the microorganism belonging to the genus Agrobacterium is introduced into the plant cell to transform the wild-type LOC_Os08g12430 gene existing in the genomic DNA of the rice with the mutated LOC_Os08g12430 gene . The microorganism of the genus Agrobacterium to be used is not particularly limited as long as it can introduce the recombinant vector into the plant cell. Preferably, however, Agrobacterium flavum containing Ti or Ri plasmid, which is disrupted, Agrobacterium tumefaciens may be used. More preferably, octopine-type or succinamopine-type Agrobacterium tumefaciens may be used, and most preferably Agrobacterium tumefaciens, Paschenes LBA4404 strain, Agrobacterium tumefaciens EHA101 strain, Agrobacterium tumefaciens EHA105 strain and the like can be used alone or in combination.

In the step (c), the callus is a cultured product produced by culturing all or a part of rice in an experimental environment. The site used to generate the callus is not limited to the stem, root, . In addition, culturing of the callus can be carried out using methods known in the art.

In addition, transformation of callus can also be carried out using methods known in the art. For example, the transformant is added to a callus, cultured at room temperature and dark to introduce a recombinant vector introduced into the transformant into callus cells, and the callus into which the recombinant vector has been introduced is cultured in a light condition to obtain a recombinant vector Can be used by replacing the mutated LOC_Os08g12430 gene with the wild type LOC_Os08g12430 gene existing in the genomic DNA of callus cells by homologous recombination to obtain a transformed callus into which the mutated LOC_Os08g12430 gene has been introduced.

Methods for screening the transformed calli include, but are not limited to, plant transformation markers such as neomycin phosphotransferase (NPT II), hygromycin phosphotransferase (HPT), chloramphenicol acetyltransferase (CAT), gentamicin, hygromycin Antibiotic resistance genes; herbicide resistance gene such as bar gene; GUS, green fluorescent protein (GFP), and luciferase (LUX).

In step (d), regeneration of the callus may be carried out using methods known in the art. For example, the transformed callus may be inoculated into MSR solid medium and cultured at room temperature under light condition to induce regeneration of callus.

As another embodiment for accomplishing the above object, the present invention provides a rice seed produced by the above method and having an increased seed yield.

The rice seeds provided in the present invention are seeds in which the expression of LOC_Os08g12430 gene is inhibited. As a result, not only the number and length of ears directly affecting the seed production of rice, but also the elongation length and stem thickness are increased, Can be increased by 20%.

According to one embodiment of the present invention, rice plants selected from 3A-00136 lines with the slowest flowering time (Example 1) were screened based on the flowering time from the T-DNA mutant group and their genotypes were analyzed. As a result, T-DNA was inserted into the LOC_Os08g12430 gene, indicating that the LOC_Os08g12430 gene was not normally expressed (FIGS. 2A to 2C). The phenotype of the 3A-00136 strain was compared with that of the control group. As a result, the kidney length was increased by about 15% (FIG. 3A) and the cell size was reduced by about 32% (FIG. 3B) , And the stem thickness was increased about 34% compared to the control (Fig. 3c). Also, as a result of comparing the yields of seeds, it was confirmed that the rice yield of the 3A-00136 strain was about 20% higher than that of the control (Fig. 4B).

Using the method of increasing the yield of seeds by inhibiting the expression of the LOC_Os08g12430 gene provided in the present invention can increase the yield of rice seeds without introducing the foreign gene, thereby eliminating the risk of introduction of the foreign gene And it can be widely used for mass production of safer rice seeds.

1 is a photograph and a graph comparing the flowering phenotype (A) of rice (HO) with flowering period (B) according to the light period condition to the control group (rice, WT of Dongjin) of the mutant strain 3A-00136.
FIG. 2A is a schematic diagram showing the position of insertion of T-DNA into the LOC_Os08g12430 gene, in which 1 indicates the amount of the LOC_Os08g12430 gene (corresponding to 6360 to 7030 in the nucleotide sequence of SEQ ID NO: 5) 3 (corresponding to 7863 to 8461 in the nucleotide sequence of SEQ ID NO: 5) of the LOC_Os08g12430 gene, and the T-DNA insertion site (3A-00136) Is 6468 of the nucleotide sequence of SEQ ID NO: 5.
FIG. 2B is a photograph showing the result of PCR amplification of the mutated LOC_Os08g12430 gene in the rice line of 3A-00136 line. The left side shows the product obtained by amplifying the mutated LOC_Os08g12430 gene and the right side shows the product obtained by mutating the mutant LOC_Os08g12430 gene And the product obtained by amplifying NGUS1 on the RB of T-DNA.
2C is an electrophoresis image showing the expression of a normal LOC_Os08g12430 gene in the wild type rice seed and the 3A-00136 lineage. The left side shows the wild type rice seed (WT) and the right side shows the rice with 3A-00136 line Represents the seed (HO).
FIG. 3A is a graph showing the results of comparing the average elongation lengths of the rice of the 3A-00136 strain with that of the control strain. FIG. 3A shows the control group and FIG. 3A shows the rice strain 3A-00136.
FIG. 3B is a microscope photograph showing cells contained in the transplanted tissue of the control and 3A-00136 lines, wherein WO represents the control group and HO represents the rice group 3A-00136.
FIG. 3C is a graph showing the results of comparing the mean stem thicknesses of the rice of the control group and the 3A-00136 strain, wherein () represents the control group and () represents the rice group of 3A-00136.
FIG. 3D is a micrograph showing a section of the stem included in the interstices of the control and 3A-00136 rice plants, wherein WO represents the control group and HO represents the rice group 3A-00136.
Fig. 4A is a photograph showing the result of comparison of the ear produced in the rice of the 3A-00136 system with that of the control, wherein WO represents the control group and HO represents the rice of the group 3A-00136.
FIG. 4B is a graph showing the results of comparing the number of seeds harvested from the rice of the 3A-00136 line with that of the control group, wherein WO represents the control group and HO represents the rice line of the 3A-00136 line.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  1: Selection and characterization of mutated rice varieties

In order to find the genes that regulate the ears, stem and flowering time, which are the traits affecting the increase in the yield of rice, by increasing the yield of rice, 100,000 rice mutants produced by the transformation method using Agrobacterium Mutants with changes in the number of grains, kidney length, and flowering time were observed in the population. Each mutant plant is a plant in which T-DNA is inserted into genomic DNA of rice and inserted by Agrobacterium, and the gene in the region is not functioning. The location of T-DNA insertion can be confirmed by genomic DNA PCR and sequencing, and by searching the gene locus id in the database, the T-DNA inserted gene can be searched. Using the above method, the plant phenotypes of the 100,000 T-DNA mutant population were observed, and 3A-00136 lines of rice with slow flowering time were selected. The selected rice was grown in a 12-hour photoperiod / 12-hour dark cycle (single condition, SD), a 14-hour photoperiod / 10-hour dark cycle (long-term condition, LD) and a paddy, (Fig. 1). At this time, rice of the Dongjin japonica which was not mutated was used as the control group.

1 is a photograph and a graph comparing the flowering phenotype (A) of rice (HO) with flowering period (B) according to the light period condition to the control group (rice, WT of Dongjin) of the mutant strain 3A-00136. As shown in Fig. 1, the stem length of the flowering phenotype was relatively large in the rice of the 3A-00136 strain, and it was confirmed that flowering was delayed in all the photoperiod conditions than in the control.

Example  2: Genotype analysis of mutated rice varieties

Example  2-1: Genome DNA  analysis

The rice seeds of the 3A-00136 strain selected in Example 1 were inoculated into the MSO medium and cultured at 28 占 폚 for 10 days. 100 mg of the leaves were collected from the cultured individuals, and the harvested leaves were immersed in liquid nitrogen for freezing and then physically pulverized to obtain pulverized material. 5 ml of 1 M Tris, 1 ml of 0.5 M EDTA, 3.73 g of KCl and 50 ml of water, pH 9.5) was added to the obtained pulverized product, and the genomic DNA of the rice was extracted therefrom. Using the genomic DNA of the extracted rice as a template, a primer (SEQ ID NO: 2 and SEQ ID NO: 3) and T-DNA (SEQ ID NO: 2 and SEQ ID NO: 3) prepared at the T-DNA insertion site in a system in which T-DNA was inserted into the first exon of the LOC_Os08g12430 gene, (SEQ ID NO: 4) capable of recognizing NGUS1 on the right border of RB (SEQ ID NO: 4) to obtain a DNA fragment in which the T-DNA present in the rice was inserted (Fig. 2B). At this time, the PCR reaction was performed at 95 ° C for 5 minutes; 38 repeats (95 ° C for 30 seconds, 53 ° C for 30 seconds and 72 ° C for 1 minute and 10 seconds); 72 < [deg.] ≫ C for 7 minutes.

3A-00136 F: 5'-tacgcatctccagagttcaa-3 '(SEQ ID NO: 2)

3A-00136 R: 5'-TTTTACTCTGTCCGTTGCAG-3 '(SEQ ID NO: 3)

NGUS1: 5'-AACGCTGATCAATTCCACAG-3 '(SEQ ID NO: 4)

FIG. 2B is a photograph showing the result of PCR amplification of the mutated LOC_Os08g12430 gene in the rice line of 3A-00136 line. The left side shows the product obtained by amplifying the mutated LOC_Os08g12430 gene and the right side shows the product obtained by mutating the mutant LOC_Os08g12430 gene And the product obtained by amplifying NGUS1 on the RB of T-DNA. As shown in FIG. 2B, it was confirmed that LOC_Os08g12430 gene and T-DNA mutated from genomic DNA of rice of 3A-00136 strain were detected.

Thus, it was found that the genomic DNA contained in the rice of the 3A-00136 strain contained the mutated LOC_Os08g12430 gene and T-DNA.

Example  2-2: mRNA  analysis

RNA iso-buffer (Takara, http://www.takara-bio.com) was added to the pulverized rice seeds of the 3A-00136 strain obtained in Example 2-1, and the total RNA ≪ / RTI > Reverse transcription PCR was performed using 2 μg of the obtained total RNA and Moloney murine leukemia virus reverse transcriptase (Promega, Madison, WI, USA) to obtain respective cDNAs. PCR was performed using primers (SEQ ID NOS: 6 and 7) capable of amplifying the LOC_Os08g12430 gene using the obtained cDNA as a template to confirm whether the LOC_Os08g12430 gene was normally expressed in the 3A-00136 strain of rice 2c). At this time, PCR was carried out using primers (SEQ ID NOS: 8 and 9) capable of amplifying the rice ubiquitin gene as an internal control, and rice seeds of the unmodified rice varieties were used as a control sample. .

LOC_Os08g12430 F: 5'-TGTTTGGTGTGGGAAGATGA-3 '(SEQ ID NO: 6)

LOC_Os08g12430 R: 5'-GCTGGATAGTTGGCCATGTT-3 '(SEQ ID NO: 7)

ubi F: 5'-AACCAGCTGAGGCCCAAGA-3 '(SEQ ID NO: 8)

ubi F: 5'-ACGATTGATTTAACCAGTCCATGA-3 '(SEQ ID NO: 9)

2C is an electrophoresis image showing the expression of a normal LOC_Os08g12430 gene in the wild type rice seed and the 3A-00136 lineage. The left side shows the wild type rice seed (WT) and the right side shows the rice with 3A-00136 line Represents the seed (HO). As shown in FIG. 2C, the LOC_Os08g12430 gene and the ubiquitin gene were normally expressed in wild type rice seeds, but the LOC_Os08g12430 gene was not expressed in the 3A-00136 rice seeds, and only the ubiquitin gene was normally expressed.

Therefore, the rice seeds of the 3A-00136 strain were mutated varieties of the LOC_Os08g12430 gene.

The results of Examples 2-1 and 2-2 show that the rice of the 3A-00136 strain obtained in Example 1 was a strain in which T-DNA was inserted into the LOC_Os08g12430 gene and the LOC_Os08g12430 gene was not normally expressed (Fig. 2A).

FIG. 2A is a schematic diagram showing the position of insertion of T-DNA into the LOC_Os08g12430 gene, in which 1 indicates the amount of the LOC_Os08g12430 gene (corresponding to 6360 to 7030 in the nucleotide sequence of SEQ ID NO: 5) 3 (corresponding to 7863 to 8461 in the nucleotide sequence of SEQ ID NO: 5) of the LOC_Os08g12430 gene, and the T-DNA insertion site (3A-00136) Is 6468 of the nucleotide sequence of SEQ ID NO: 5.

Example  3: phenotypic analysis of rice from 3A-00136 strain

The phenotype of the 3A-00136 strain of rice obtained in Example 1 was compared with the outline of the unchanged Dongjin rice (control group) to analyze its phenotype.

First, the elongation length from the end of the stem of each rice to the end of the ears was measured and compared (Fig. 3A).

FIG. 3A is a graph showing the results of comparing the average elongation lengths of the rice of the 3A-00136 strain with that of the control strain. FIG. 3A shows the control group and FIG. 3A shows the rice strain 3A-00136. As shown in FIG. 3A, the average elongation length of the control group was about 90 cm, while the average elongation length of the rice group 3A-00136 was about 110 cm. Therefore, it was found that the rice length of 3A-00136 strain was about 15% longer than that of the control.

Secondly, in order to observe the size and number of cells contained in each rice, a third internode of each rice in which flowering is over 30 days has been obtained, and the obtained intergranular tissue is transferred to FAA fix solution and immersed in formalin (35%): acetic acid: alcohol (70%) = 1: 1: 18 to fix cells contained in each interstitial tissue. The fixed interstellar tissue was applied to a LEICA RM2265 section instrument and cut to a thickness of about 1 mu m. The interspersed tissue was stained with a 0.05% toluidine blue solution, and then stained with a 20-40 magnification optical microscope (Fig. 3B).

FIG. 3B is a microscope photograph showing cells contained in the transplanted tissue of the control and 3A-00136 lines, wherein WO represents the control group and HO represents the rice group 3A-00136. As shown in FIG. 3B, it was confirmed that the cells observed in the interstitial tissue of rice of 3A-00136 strain were smaller in size than the cells observed in the transplantation tissues of the control group, and the number of cells contained in the same range was increased, As a result, it was confirmed that the number of cells decreased about 32%

Third, the stem thickness of each stem was measured and compared at a position 4 cm above the nosepiece 1 to 6 of each intergranular tissue using a vernier caliper (Fig. 3C).

FIG. 3C is a graph showing the results of comparing the mean stem thicknesses of the rice of the control group and the 3A-00136 strain, wherein () represents the control group and () represents the rice group of 3A-00136. As shown in FIG. 3C, it was confirmed that the average stem thickness of rice of 3A-00136 strain was increased more than that of the control group, and it was confirmed that the stem thickness was increased by about 34% compared with that of the control stem.

Fourth, in order to observe the stem cross-section of each rice, a third internode of each rice after 30 days of flowering was obtained, and the obtained intersexual tissues were immersed in FAA fix solution, The cells contained in the interstitial tissue were fixed. The fixed interstellar tissue was applied to a LEICA RM2265 section instrument and cross-cut to a thickness of about 1 mu m. The cut interstices tissue was stained with 0.05% toluidine blue solution and then observed with an optical microscope at 20-40 magnification 3d).

FIG. 3D is a micrograph showing a section of the stem included in the interstices of the control and 3A-00136 rice plants, wherein WO represents the control group and HO represents the rice group 3A-00136. As shown in FIG. 3D, it was confirmed that the area of the stem cross-section observed in the intergranular tissue of the rice of the 3A-00136 system was larger than that of the stem cross-section observed in the transgranular tissue of the control group.

The results of FIGS. 3A to 3D are summarized as follows. When the function of the LOC_Os08g12430 gene is inhibited, not only the elongation of stem but also the development of thickness are accelerated, thereby promoting overall stem development.

Example  4: Seed productivity analysis of rice from 3A-00136 strain

First, the ear and the grain of the 3A-00136 strain obtained in Example 1 were compared with those of the control group (Fig. 4A).

Fig. 4A is a photograph showing the result of comparison of the ear produced in the rice of the 3A-00136 system with that of the control, wherein WO represents the control group and HO represents the rice of the group 3A-00136. As shown in FIG. 4A, it was confirmed that the length and number of the ear were increased in the rice of the 3A-00136 strain compared to the control.

In addition, the number of seeds harvested from rice of the 3A-00136 strain was compared with that of the control (Fig. 4B).

FIG. 4B is a graph showing the results of comparing the number of seeds harvested from the rice of the 3A-00136 line with that of the control group, wherein WO represents the control group and HO represents the rice line of the 3A-00136 line. As shown in FIG. 4B, it was confirmed that the number of harvested seeds was increased by about 20% in the rice of the 3A-00136 strain compared to the control.

4A and 4B, when the function of the LOC_Os08g12430 gene is inhibited, it is analyzed that the number of seeds increases as well as the length and number of abnormalities.

<110> University-Industry Cooperation Group of Kyung Hee University <120> Novel rice stripe with high productivity of rice seed and process          for preparing the same <130> KPA140670-KR <160> 9 <170> Kopatentin 2.0 <210> 1 <211> 9252 <212> DNA <213> Artificial Sequence <220> <223> recombinant LOC_Os08g12430 <400> 1 acgatcttgg ttcgcttcgt gcgactcctc ctcctcttgc gccggtgccg acctcgcctc 60 gcctcgcctg cgtcgcgtcg atctcgccag cggcggcgcc agccggccgg cgtctgctgc 120 gtcctccgca tcctcgctcg ccggtgagca tccatggcca tgctcattct catctcgctc 180 tacccgcgct gtttttttcg aattcgcgtt agccctagct tttcttctag atctggaagc 240 agcttttctc tttgcacttt ttgcgttagc ccttgcaata acaatagtaa cagtttaatc 300 tgttcccaat agagaatttt ggggttcaac cgaaccctca tgtcccagct aggtccgccc 360 ctgtcagtgc gactgccttg ttgcaacttt tttttttgtt tttggttata ctcctatcaa 420 ctattcctga gtgtacaatg cgataagatt ttgttccctt ccctgcagag agttggggtc 480 aagcaaagtt ggttgttttt tactcgaatg tcgtgaggaa caccgaggaa tcctccagtt 540 tcgcatggta gggtccaatg cctttttctg actaaaaagc tgttggtttt gttaagtgtt 600 gttatcacca ttttatgaaa tgtttacatg tgcccaacaa cttagtatta tcgttttctc 660 caaccatgca gagacccata tgttgaacaa aggtgaagct gtgctgtgga gacctgataa 720 aattttctaa taatgcttgt gcaaatgaag aatactgttc atctcacgct attcctgtgt 780 tctttctcaa gaatgctttg ctcctattct tttattgaaa tcaatttgtg ttattatctt 840 cccattcgta aggtgctgac tagttcactt ttttatgtca tagagtagga aggttctctg 900 tactgcaagt gtttggattt cctcgagccg aatgagcttt cactggtgga tgtggtaata 960 aaagtccctg ttttcaggta tacgctgact gtagtgtttt ggggatatac gctacactaa 1020 taatagagaa cacatacaaa ttaatcccta taagtgaact ggaactactg tgttattagt 1080 gtgttggcca gatttggatg actaaggtca aatgctaaaa atcatttgga atctttcaaa 1140 aaaggaccat ttggattact attccgtaca tgctctcagg cccttccacg ttctactttt 1200 ctaatggcat aagtaatgta tctgccttga tttgcaccac caatatcagc taatacttta 1260 gttcctgttc catctcctga aaacaatggt ccttcaattt cgcagtatca gtcctttcac 1320 tgtatgcttt cctacccaag cttctccttc atatatttaa aagaatgggt aaatatagaa 1380 cagaataaag tggctacact atggatcagt ccggtgaaca attattgtga cacagttttt 1440 ttcccaataa actgggcatg tcttgtgaaa cagagtgatc tctgtcttta tgatagacaa 1500 ataactatgt tggagtttgc tagaactact tgtaaggaag atgccattaa gtgaccacct 1560 gaaatcgtgt tgtagtgtca ttccataaca tttatttaag atttctccaa tggaagttgt 1620 atctaaatat gcctaatatt ttgcatatgt agtgtttgct ctttggaatt ataaatcatt 1680 ctaggatgct gttccaatca gtttgggaag ctcaggtttt catcaaagac gattagtaaa 1740 atttctgtca gtctgcaaaa tttgtggtat ggtaaaattc ctttaaagta tagaaaattt 1800 aagtgacaa acaaaagtta gggccattgt atattacatc aaaagattgt ttcattctgt 1860 tgctatcaat cttaaataac gatttcaggg tgcaggatcc aggttcccat tgggccacgt 1920 ggcactttac aaatttttgt aaggctactc tgaaattgtg ccacgtggcc tcccctatgc 1980 atatttaaac tgcattctcc tgtccactga actgttagga aaactctgga gtcaataaat 2040 cacctttgcc tgacccttgt gaagaagtta gagatgtaac ccatccgaag tctctagaaa 2100 aattaaggta ttacatggtc cttcacgtgt attaagtata ggcagtcaaa aaggtaaaat 2160 tctctacacg cacaaactat ttcagtactc acatagtcac atattgattt ccaagacctt 2220 ggatgcttta acttttcaag actggtttta acttttaata caaagagtaa tcttttatat 2280 atggctctgc gtattaagct gacaattaga gtcatttgcc taaaaagtta cgccttaaat 2340 aaatttcgta attttcctat aggttgacaa attctgtaaa tcatactaat tcaaaagaca 2400 catcttccaa agtatttgtt caaggtatgt atatacgctt aaacaaatta atcttccatt 2460 agcaaaattt gttttcaggt tgaattataa ttatacacca gacaagtaca gctagctaaa 2520 tgcggcagag aagaattagc catgcataaa ctaattaacc atattactct atttgttgat 2580 aatattgctc taattatgtt ccattgtacc tatgattagc ttatattatt tttaagatgt 2640 ggggggtatc gtatatgaga ctacagaggg gactaggtga cactggtaga ttagcacaag 2700 aaatgtctct tagagagact tgtcaaaaac tatatccttt agttttcagt ccactaatca 2760 tctaatctta ctaattatta ataggtgtta atcactctgt ctaatggaag agaaaaacaa 2820 aggtttgaaa gtaaataggc aagtactaca cataattttg ctttctgtat taattctttt 2880 acaatgctta aatctttaca taaatacggt tattattaca ggttaatcgt tcgtcaacct 2940 tttcaaaatt cattaaatag gttcttctaa atctacaagt gtatagattt tttttaaaga 3000 aaagaaatta tcaagtactg catttgaaac tttaaacaac aacaacaata attgttctaa 3060 ataagtaaat atatcatagc tataagttgg tgtgccgatg gtgcgccaat tttctacgat 3120 atggaagcgg cgaattggga aaggataggg tggtgagatt ttatatttgt ttctaatttt 3180 tagggtattc agtgtaatgt tggactactc tataaatttg aaaaacatat ggactaaagt 3240 aagaaatatg attgcccgac atgaaaaatc aaagctatct ctagagttac tttgcattat 3300 gggataaaca aatttgagac atgttacctc atcctacatg atactttgag gtcctttata 3360 ggagggtctt gcactatgaa tgtccaaaga gaggtggtat agctatagtt ttccgaatta 3420 tacatatgga gctacgaatt atgacatcta aatggtgtac tacttgcaat agaagatatg 3480 gaggcgtgtc cctatccatt cacatgtggc tcttcaaata gcacgtgaac tttgttgccc 3540 tctcgtagtc atgacaatat tttcgactac tgagaagata agcaaatatt gagaaaaaaa 3600 agagagagcg tggtagatat acaaatgata agatttttaa ccatcaaaat aatggattta 3660 ataatcctaa agtaaaagaa atagagagaa tttaaaaggt tataattagg ccgaaagaaa 3720 ataaaaatat gggaagaagg aaaagaacaa aatattttcc atttctttca taaccagtta 3780 aaaaaacaat ccaactgtta gggcaattgt aagaaacctc taattattga agtcccaaac 3840 gattttcata taaaacttcg ccttttagcc ttatcttatt aataataatc aaattgacca 3900 tcaaccaaca tatatttgac atcattcttt ttttcctctg taatttaaat atttacaaat 3960 ataatgtttt cataaaattg aaaaactgct aaaagatctc tttaaaatgt tacgctttta 4020 cgaataacaa taaatagtac gtgtagtata aatatggttt gctatatgga tggtgcgcta 4080 acggcgcacc aattttctag tgttatctaa ttgctgatcg atcctgatcg ccaagggacc 4140 gatcaggtca attcatcgtc gatcagcctt gattcatcgt cgatcagcct tgattaatcg 4200 acctgatcga tactatgtat accagaacct atattctata tagtgcacaga tataaagcaa 4260 tcacagtgca taattgatgg caggatggtc aattacttgc cgtagtataa agcaatcatt 4320 gtataaagca accagcagta gtagcacatc ttcacatgtg ctaaacccta agacttgttc 4380 taaaaatgat cataggtgct ctgatcagac gattaatcgc gcctgattga cgattaaccg 4440 gacgatcaga tttctgatcg aatgaatgtc cctaatcgga ttcaggggat tgatcaggac 4500 aattaattgc gattaatcgg acgatcagat aacactgatt gctatagcaa tagcagttgc 4560 tttcctctct ccagtctcca ccataggttg tgtggagtcc acccaagaat ttctcttgtt 4620 cccttgttca ctctttcttt ttttcttcgt ccgtgtttat ctacttttgt tgaatcgaca 4680 gtctggatt tgttggccag ttggtagact catggttttt aaggcagtaa 4740 ggcgtcctaa ggcgaccccg gactgcctta tcgccatagc ggtaagacgt aaggcgaggc 4800 gacaccttag gcactccata ctaattaggt ggcaaacaaa ataatctaat atgctagcaa 4860 atcaaagttg cattttaaga gcagtacaac gcgcatatat ataccttgtt cttggcaact 4920 cttgagccca ctagatggtt ataataatta cttaacaaga tatcaattta ttaactacta 4980 acagggagtc agggatgcag gggaggggca gggagtaaaa cagagcagta gaggaaggag 5040 tttagtagat gaaatgtaga ggagagagag gaaagaccac aacatagtac tctctccgtc 5100 cctaaatatt tgacgccgtt gactttttga aatatgtttg acagttagtc ttattcaaaa 5160 aatttaagta attattaatt cttttcctat cattgattca ttgttaaata tactactact 5220 actcaagttt ttgaataata ttcacaaaag tttttgaata agacgaatgg tcaaacatgt 5280 ttaaaaaagt caacggcgtc aaatatttag ggacaaaggg agtggcaaaa gagggagaag 5340 agtacatagg atgtagaaga ggggggagga aacaccaaaa cagagcagca gaggaaggat 5400 aagagcagag cagaggaagg tgcaaagcag gatcacgtag agcagagaaa gaaacagagg 5460 aaagtagagg gtggagggaa gagaaagaag agcatgcccg cttagttttt gtgtcaatgg 5520 aggaccagga tgatggcgca ggtgccgagg accaggatga tggcgcaggc acaggatagg 5580 cacatgtgac agctgggatt gccgctaggg atggagaagt caccagggat ggatcgccat 5640 cgccacagcc tcaggctcca gcgaccctct ttctgttgtt ttctccctga tttggtggga 5700 tttggtgcgt ggggaccttt tcacccctaa gattggcgct tctgttagtt ctgtttcgtg 5760 cccagggccg caccaatttt tttgggccct tcaaccaatc gcggcaaaaa ctgcgccttc 5820 aacttcctct ggcgtcgcct ttaaccgttg tagcacctaa ggacgtgata tcgacgtcat 5880 agcgacgcct tgggcataag gcggacgcca taaacaatat ggtaaggcgg gctagacgca 5940 gtgactcctg gcactggcag cgccttacct ccatagcgac gctatggcga cgccttgaca 6000 cttaattttg gatttttttt aactgtagta caaaatacaa gttattataa aatcccaact 6060 aaattgattg agttaaattg ggttttggtt gacttacgaa gttatggtca tccattggat 6120 tgttgccagg tagtcagctg attgtgtata tgactcaact atacattttg tttgttggta 6180 tcagtatatg atactatcac ttattgtgtc ctttagatgt caatctgcat ttttactctg 6240 tccgttgcag cacacttact tttctttctt tatttgtata tactcatctc tattgaatcc 6300 ttgctagatg gtatttgcta taggcctgga cctcattatt ctattttcca ggatttttca 6360 tggacccggg aaaacacaat ggaatgagtc tgaaggacag aagagaatta gtatatgaag 6420 tttcacaatg gccacaaggt gcctctgaga ttttacaatg ttggactcgc agagatcttc 6480 ttgagcttat ctgtgctgag ttgggcaagg agaggaaata cactaatgtt ccaaaatcta 6540 aaatgattgc ttatctgtta aaattagttt tacgaaagaa tggacagcca aaggatgata 6600 atgcaaatgc ttcaattttg gggcagaata acaaagatga cacagagaag aaagaaaatg 6660 aggaacagcc acatcatttc agcagaagtg caaagtctga ctcatcaatg tgcagagagg 6720 cccaagctgg tagcacagcg gtatgccgaa atgttgcatg ccaggctact ttgaactctg 6780 gagatgcgta ttgcaagcgt tgctcttgct gcatttgcca taaatatgat gagaacaaag 6840 atcctagctt atggttggtt tgcagctctg atacaccgta ctctggctat tcatgcggca 6900 catcatgcca cctaaagtgt gctctaaaga ataaaaaagc tggcattttc aagaatgggt 6960 gcaacaagaa gtcagatggt agtttttact gtgtttggtg tgggaagatg aactggctaa 7020 tgaggtatct ctttgctatt ttaattataa cattccagtt atttatgtgg aaacaaacac 7080 atctaaaatt gtgttctgca tgttatccta attctgaagt agcatggtgt ggtactgatc 7140 cctgaccaaa actgtccttt ttttttggta gcatgcattg ctcagcttta atatcatgtt 7200 tttgtgttaa gcaattcatc tgatacttta tatattgaaa atttgtgcca gttttacaat 7260 ctacccattc tcattttggc acttacaatc ttggtcttgt attaaaaaat caagagtatt 7320 cacagtgaaa ttttacctct agatttcacc ttttacatca ttgcaggaat ctgcgtaagc 7380 agctggcaat tgcaagagaa tctagaagag ttgatgtact gtgtgagcgt ttgtcactga 7440 ctcataaaat ggtgaaaggg tctgaacgtt acagggaact ggcaaacata atcaactcag 7500 ctgtgaaaat acttgagaaa gaagttggtt gtgcattaga tcaagtgtct gcaatcacgg 7560 ggcggggcat cgtgaaccgg ctttgttgtg gtgctgatgt tcaaaaactt tgttcatgtg 7620 cactagaaat ggtagactcc acattgagta gcaccctgga cttcgaaaca aacaataatc 7680 tggaagcgcc aggtaatttt gtgatagtat tgactcacac aaaactgcag ttctactccg 7740 caaaattatc cctgcagaac taggcattgt tatgcaaaga gttatttatg atcttccaat 7800 gtttggata ctacgacaat gatgtattga cattgtgtgt gaagagattt tatccacttt 7860 tgcaggtccc cagcctcaag tattttttgt ggagattact ccattttctg tgcttgttgt 7920 attgaagtac caggataata tcgctgagga gattgatggt tgcaaagttt ggcaccgaag 7980 tgcaaacatg gccaactatc cagcagagcc cacttgccac gttcttagac caaatacaag 8040 gagtcttttt tcaggtctaa gcccttctac tgagtatttc ttcaaggttt tgccttttgg 8100 ctgttcacag ggatatggcg aatgggaggt aaaatgctct acacgaagcc ttaaccatgg 8160 cagcagccag tgctcaacac agaattccga gagtatgtcc atcaaggagg atttggagca 8220 gcatcagaaa aatgagttga atctgaagaa caagcagtgg tggggcattc aatatgactc 8280 accctcggca aactcgaatg agaacgatgt gtgtcctgac ctgcacccta agcgagctaa 8340 gctcgcaaag ttggacggtg caagtgacaa cgacgaaagc cagttgctgc caacatcaga 8400 agttttacca ttcatgagct ctaattcttc actttcagaa gttccaagca aacctgactg 8460 ggttagcagc accccagatt ccgcctgcaa gaaccatgtt gagaggcagt atgaatacag 8520 tgtgaaggtc attagatggc tggagcatga agggcacatg gacaaggatt tccgcgtgaa 8580 gttcctcaca tggtttagcc tgaaggcgtc ggcacaggag cgacgcattg tgaacgcctt 8640 cgtagatgct ctggtgagtg atcctgcgag cctggtagca caactgattg attcgtttat 8700 ggaggttgtc tgcagcaagg agaagcctgc tcagccgaat ggtgggtgct gcaacctttg 8760 gcattagaaa gttccattgc cactgaggaa tctcactatc atatttttgc caataatcat 8820 ctgcatcgaa aagctgagca attcaagcta tggcttattg ctttatttga tcaatttaac 8880 actagttgct gattgctagg tttctgaact gtggtggtgg tggtggtggt tatgttaggt 8940 gtatgaacta agctgaagtt gttgacaagt gattcctgga atgctataaa tggaggtagg 9000 tttagatgct tcaaaactgg tatcatctta tccataatga tgatatatca tttgtatttt 9060 ccccaaatct cgttgcttag tgattgctga cataatcttc tttagttaca ccagttttat 9120 gaactaggcc taagttggtt acactctctt ggagccaaac tttgcctagc taatgctcat 9180 ctgcgattgt tcagtgactg tagacgagca accgtatgca tctagtggaa atttcgttgt 9240 tggtttgcaa ga 9252 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 3A-00136 F <400> 2 tacgcatctc cagagttcaa 20 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 3A-00136 R <400> 3 ttttactctg tccgttgcag 20 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> NGUS1 <400> 4 aacgctgatc aattccacag 20 <210> 5 <211> 17527 <212> DNA <213> Artificial Sequence <220> <223> mutated LOC_Os08g12430 <400> 5 acgatcttgg ttcgcttcgt gcgactcctc ctcctcttgc gccggtgccg acctcgcctc 60 gcctcgcctg cgtcgcgtcg atctcgccag cggcggcgcc agccggccgg cgtctgctgc 120 gtcctccgca tcctcgctcg ccggtgagca tccatggcca tgctcattct catctcgctc 180 tacccgcgct gtttttttcg aattcgcgtt agccctagct tttcttctag atctggaagc 240 agcttttctc tttgcacttt ttgcgttagc ccttgcaata acaatagtaa cagtttaatc 300 tgttcccaat agagaatttt ggggttcaac cgaaccctca tgtcccagct aggtccgccc 360 ctgtcagtgc gactgccttg ttgcaacttt tttttttgtt tttggttata ctcctatcaa 420 ctattcctga gtgtacaatg cgataagatt ttgttccctt ccctgcagag agttggggtc 480 aagcaaagtt ggttgttttt tactcgaatg tcgtgaggaa caccgaggaa tcctccagtt 540 tcgcatggta gggtccaatg cctttttctg actaaaaagc tgttggtttt gttaagtgtt 600 gttatcacca ttttatgaaa tgtttacatg tgcccaacaa cttagtatta tcgttttctc 660 caaccatgca gagacccata tgttgaacaa aggtgaagct gtgctgtgga gacctgataa 720 aattttctaa taatgcttgt gcaaatgaag aatactgttc atctcacgct attcctgtgt 780 tctttctcaa gaatgctttg ctcctattct tttattgaaa tcaatttgtg ttattatctt 840 cccattcgta aggtgctgac tagttcactt ttttatgtca tagagtagga aggttctctg 900 tactgcaagt gtttggattt cctcgagccg aatgagcttt cactggtgga tgtggtaata 960 aaagtccctg ttttcaggta tacgctgact gtagtgtttt ggggatatac gctacactaa 1020 taatagagaa cacatacaaa ttaatcccta taagtgaact ggaactactg tgttattagt 1080 gtgttggcca gatttggatg actaaggtca aatgctaaaa atcatttgga atctttcaaa 1140 aaaggaccat ttggattact attccgtaca tgctctcagg cccttccacg ttctactttt 1200 ctaatggcat aagtaatgta tctgccttga tttgcaccac caatatcagc taatacttta 1260 gttcctgttc catctcctga aaacaatggt ccttcaattt cgcagtatca gtcctttcac 1320 tgtatgcttt cctacccaag cttctccttc atatatttaa aagaatgggt aaatatagaa 1380 cagaataaag tggctacact atggatcagt ccggtgaaca attattgtga cacagttttt 1440 ttcccaataa actgggcatg tcttgtgaaa cagagtgatc tctgtcttta tgatagacaa 1500 ataactatgt tggagtttgc tagaactact tgtaaggaag atgccattaa gtgaccacct 1560 gaaatcgtgt tgtagtgtca ttccataaca tttatttaag atttctccaa tggaagttgt 1620 atctaaatat gcctaatatt ttgcatatgt agtgtttgct ctttggaatt ataaatcatt 1680 ctaggatgct gttccaatca gtttgggaag ctcaggtttt catcaaagac gattagtaaa 1740 atttctgtca gtctgcaaaa tttgtggtat ggtaaaattc ctttaaagta tagaaaattt 1800 aagtgacaa acaaaagtta gggccattgt atattacatc aaaagattgt ttcattctgt 1860 tgctatcaat cttaaataac gatttcaggg tgcaggatcc aggttcccat tgggccacgt 1920 ggcactttac aaatttttgt aaggctactc tgaaattgtg ccacgtggcc tcccctatgc 1980 atatttaaac tgcattctcc tgtccactga actgttagga aaactctgga gtcaataaat 2040 cacctttgcc tgacccttgt gaagaagtta gagatgtaac ccatccgaag tctctagaaa 2100 aattaaggta ttacatggtc cttcacgtgt attaagtata ggcagtcaaa aaggtaaaat 2160 tctctacacg cacaaactat ttcagtactc acatagtcac atattgattt ccaagacctt 2220 ggatgcttta acttttcaag actggtttta acttttaata caaagagtaa tcttttatat 2280 atggctctgc gtattaagct gacaattaga gtcatttgcc taaaaagtta cgccttaaat 2340 aaatttcgta attttcctat aggttgacaa attctgtaaa tcatactaat tcaaaagaca 2400 catcttccaa agtatttgtt caaggtatgt atatacgctt aaacaaatta atcttccatt 2460 agcaaaattt gttttcaggt tgaattataa ttatacacca gacaagtaca gctagctaaa 2520 tgcggcagag aagaattagc catgcataaa ctaattaacc atattactct atttgttgat 2580 aatattgctc taattatgtt ccattgtacc tatgattagc ttatattatt tttaagatgt 2640 ggggggtatc gtatatgaga ctacagaggg gactaggtga cactggtaga ttagcacaag 2700 aaatgtctct tagagagact tgtcaaaaac tatatccttt agttttcagt ccactaatca 2760 tctaatctta ctaattatta ataggtgtta atcactctgt ctaatggaag agaaaaacaa 2820 aggtttgaaa gtaaataggc aagtactaca cataattttg ctttctgtat taattctttt 2880 acaatgctta aatctttaca taaatacggt tattattaca ggttaatcgt tcgtcaacct 2940 tttcaaaatt cattaaatag gttcttctaa atctacaagt gtatagattt tttttaaaga 3000 aaagaaatta tcaagtactg catttgaaac tttaaacaac aacaacaata attgttctaa 3060 ataagtaaat atatcatagc tataagttgg tgtgccgatg gtgcgccaat tttctacgat 3120 atggaagcgg cgaattggga aaggataggg tggtgagatt ttatatttgt ttctaatttt 3180 tagggtattc agtgtaatgt tggactactc tataaatttg aaaaacatat ggactaaagt 3240 aagaaatatg attgcccgac atgaaaaatc aaagctatct ctagagttac tttgcattat 3300 gggataaaca aatttgagac atgttacctc atcctacatg atactttgag gtcctttata 3360 ggagggtctt gcactatgaa tgtccaaaga gaggtggtat agctatagtt ttccgaatta 3420 tacatatgga gctacgaatt atgacatcta aatggtgtac tacttgcaat agaagatatg 3480 gaggcgtgtc cctatccatt cacatgtggc tcttcaaata gcacgtgaac tttgttgccc 3540 tctcgtagtc atgacaatat tttcgactac tgagaagata agcaaatatt gagaaaaaaa 3600 agagagagcg tggtagatat acaaatgata agatttttaa ccatcaaaat aatggattta 3660 ataatcctaa agtaaaagaa atagagagaa tttaaaaggt tataattagg ccgaaagaaa 3720 ataaaaatat gggaagaagg aaaagaacaa aatattttcc atttctttca taaccagtta 3780 aaaaaacaat ccaactgtta gggcaattgt aagaaacctc taattattga agtcccaaac 3840 gattttcata taaaacttcg ccttttagcc ttatcttatt aataataatc aaattgacca 3900 tcaaccaaca tatatttgac atcattcttt ttttcctctg taatttaaat atttacaaat 3960 ataatgtttt cataaaattg aaaaactgct aaaagatctc tttaaaatgt tacgctttta 4020 cgaataacaa taaatagtac gtgtagtata aatatggttt gctatatgga tggtgcgcta 4080 acggcgcacc aattttctag tgttatctaa ttgctgatcg atcctgatcg ccaagggacc 4140 gatcaggtca attcatcgtc gatcagcctt gattcatcgt cgatcagcct tgattaatcg 4200 acctgatcga tactatgtat accagaacct atattctata tagtgcacaga tataaagcaa 4260 tcacagtgca taattgatgg caggatggtc aattacttgc cgtagtataa agcaatcatt 4320 gtataaagca accagcagta gtagcacatc ttcacatgtg ctaaacccta agacttgttc 4380 taaaaatgat cataggtgct ctgatcagac gattaatcgc gcctgattga cgattaaccg 4440 gacgatcaga tttctgatcg aatgaatgtc cctaatcgga ttcaggggat tgatcaggac 4500 aattaattgc gattaatcgg acgatcagat aacactgatt gctatagcaa tagcagttgc 4560 tttcctctct ccagtctcca ccataggttg tgtggagtcc acccaagaat ttctcttgtt 4620 cccttgttca ctctttcttt ttttcttcgt ccgtgtttat ctacttttgt tgaatcgaca 4680 gtctggatt tgttggccag ttggtagact catggttttt aaggcagtaa 4740 ggcgtcctaa ggcgaccccg gactgcctta tcgccatagc ggtaagacgt aaggcgaggc 4800 gacaccttag gcactccata ctaattaggt ggcaaacaaa ataatctaat atgctagcaa 4860 atcaaagttg cattttaaga gcagtacaac gcgcatatat ataccttgtt cttggcaact 4920 cttgagccca ctagatggtt ataataatta cttaacaaga tatcaattta ttaactacta 4980 acagggagtc agggatgcag gggaggggca gggagtaaaa cagagcagta gaggaaggag 5040 tttagtagat gaaatgtaga ggagagagag gaaagaccac aacatagtac tctctccgtc 5100 cctaaatatt tgacgccgtt gactttttga aatatgtttg acagttagtc ttattcaaaa 5160 aatttaagta attattaatt cttttcctat cattgattca ttgttaaata tactactact 5220 actcaagttt ttgaataata ttcacaaaag tttttgaata agacgaatgg tcaaacatgt 5280 ttaaaaaagt caacggcgtc aaatatttag ggacaaaggg agtggcaaaa gagggagaag 5340 agtacatagg atgtagaaga ggggggagga aacaccaaaa cagagcagca gaggaaggat 5400 aagagcagag cagaggaagg tgcaaagcag gatcacgtag agcagagaaa gaaacagagg 5460 aaagtagagg gtggagggaa gagaaagaag agcatgcccg cttagttttt gtgtcaatgg 5520 aggaccagga tgatggcgca ggtgccgagg accaggatga tggcgcaggc acaggatagg 5580 cacatgtgac agctgggatt gccgctaggg atggagaagt caccagggat ggatcgccat 5640 cgccacagcc tcaggctcca gcgaccctct ttctgttgtt ttctccctga tttggtggga 5700 tttggtgcgt ggggaccttt tcacccctaa gattggcgct tctgttagtt ctgtttcgtg 5760 cccagggccg caccaatttt tttgggccct tcaaccaatc gcggcaaaaa ctgcgccttc 5820 aacttcctct ggcgtcgcct ttaaccgttg tagcacctaa ggacgtgata tcgacgtcat 5880 agcgacgcct tgggcataag gcggacgcca taaacaatat ggtaaggcgg gctagacgca 5940 gtgactcctg gcactggcag cgccttacct ccatagcgac gctatggcga cgccttgaca 6000 cttaattttg gatttttttt aactgtagta caaaatacaa gttattataa aatcccaact 6060 aaattgattg agttaaattg ggttttggtt gacttacgaa gttatggtca tccattggat 6120 tgttgccagg tagtcagctg attgtgtata tgactcaact atacattttg tttgttggta 6180 tcagtatatg atactatcac ttattgtgtc ctttagatgt caatctgcat ttttactctg 6240 tccgttgcag cacacttact tttctttctt tatttgtata tactcatctc tattgaatcc 6300 ttgctagatg gtatttgcta taggcctgga cctcattatt ctattttcca ggatttttca 6360 tggacccggg aaaacacaat ggaatgagtc tgaaggacag aagagaatta gtatatgaag 6420 tttcacaatg gccacaaggt gcctctgaga ttttacaatg ttggactcgt ttacccgcca 6480 atatatcctg tcaaacacgg atccgaggta ccaggtacca ggtgagttcc attcttacta 6540 ccacggtgct attttttttg ctatgtggct aattacatga ctaacttggg gtgctaaatc 6600 ttacaggtta tatgcaggtt atatgcaggt cccgggtagg tcagtccctt atgttacgtc 6660 ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg 6720 atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg 6780 caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt cgtaattatg 6840 cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca ggccagcgta 6900 tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat aatcaggaag 6960 tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg tatgttattg 7020 ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg cagactatcc 7080 cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt 7140 tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg aacacctggg 7200 tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg tctgttgact 7260 ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg 7320 ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac ctctggcaac 7380 cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca gagtgtgata 7440 tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggccaacag ttcctgatta 7500 accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac ttacgtggca 7560 aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg attggggcca 7620 actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg gcagatgaac 7680 atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct ttaggcattg 7740 gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc aacggggaaa 7800 ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa aaccacccaa 7860 gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaagtg cacgggaata 7920 tttcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca 7980 atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc 8040 tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac 8100 tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat 8160 acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt 8220 atcgtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg 8280 gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg 8340 gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc 8400 aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaatgaa 8460 tcaacaactc tcctggcgca ccatcgtcgg ctacagcctc gggaattgct accgagctcg 8520 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc 8580 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac 8640 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac 8700 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg 8760 gtgtcatcta tgttactaga tcgggaatta attcatcgat aggctagtca tggtgactgt 8820 acgttgtaag tgcagcaaac tgccgacgcg atgcaaactg tacacgttaa catgccactc 8880 acctggaacg cacaatggcc actaggtgcg gccgtagtgt ggatttcaaa gagagagaga 8940 gagagagaga gctaatcacg taaacgtaaa cacagcagat agcagagatg ttgattaggc 9000 aaaacagtat aaaagccaat ccaataaact acatttagcg aagtgctata ctaatgcact 9060 aataacgaac tgttcttttc ttaagatcgg agccagtaat gggttgtcag caggagaagc 9120 acgtaaacct tgaaacatac taagttccac agtcgagagt aaaccgtaat caacacaaga 9180 aacaaacata aaattgaaca aacgcgcata ttataagtga cgaagcggtc tcacataaaa 9240 cagggcacac aggttacaac aacgagggtt gtaagcccat taagccccaa acatcagatc 9300 accacaagca aatgtctcga agacacacgc acacggcaac aggataactc cacactggca 9360 gatcatggga tagcagcagt tatcaatcag gccttgacac acagaacatc aagcccccag 9420 acgacgacga ctcctctaga tcccggtcgg catctactct attcctttgc cctcggacga 9480 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg 9540 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt 9600 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga 9660 tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc 9720 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc 9780 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca 9840 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc 9900 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc 9960 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca 10020 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat 10080 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg 10140 accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt 10200 gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc 10260 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca 10320 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca 10380 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc 10440 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctca ttgccccccg 10500 ggatccgtcg agtcagcctg aaaggacaaa atacatgtta gcgccttagt ggtacattat 10560 tatttcagta cagcaagata acacaattca aaagactgac ccataataaa taactagtcc 10620 tcaatttaaa atttgagttc ctaaatagac atctatgaat atgctgtaca tcggcactac 10680 agaaaatacg attcccaata attgaacaat tgtactttat ttagttgtta ctacaacaat 10740 ggaagataca agatcgtttc aaaactacca tacatgcatg gagtatttgt tccacagatc 10800 tggaaaaaac agatctgacg ggcagtgtca ccaaacacta gacatatgta tttgtattag 10860 gtggatgacg tgtacaaaca tgactaccag atctagaatt agaacgcggc gtgctttgga 10920 aatgttaagt agatccaaat acatcggtaa caaatgaaca tattcatatg acatagctgt 10980 aaaacatcat gatctatcat atcaactaga ggggatctcg gcatgtatca ttctattgca 11040 tctagaacca tagcatttcc atgtgacaaa caattttgaa acatacattg ctcagatcta 11100 ccataagaac accatgttca tgacagcatc gaccatgatt tccacattta acagcatccc 11160 gaggtcagat ccagtgttta gaactatcat ttcagcaaaa ttcacaaaat aaatcctcca 11220 attcgcctac atatatccat tccacgcatc ctaggaccag atccaccata ccagtataca 11280 cgaacactaa acatttcatc agatccgcta actacgacag agaaaacgca gatccagaca 11340 accagatcca ccgacaaata aacacagccc cccacatcca acaaccgcga atccacccag 11400 atctgaccca gaagcaggca cgaagaacac ggggggtgag ggagatggga cgcggatcca 11460 agcttggcgg cggattgggt tgatgctgcg acggcggcgg agaagggaga gaggggagag 11520 gaggaaaagc gcggaggcgc ggagagaggc gagtacgaag acgcctttct ctgcgttgtc 11580 tcggcttagg gtttgcgatc cccgcactcc gcccgtttta tagggccaga cagcctggac 11640 ctctcaggag cggaagcgaa gggatggggg agtttttcgt tttaccccct ttgacgtctt 11700 caaaattcac tcgcatctca catcgtgcct gcaaaggtcg ggtggttgga tcatatcgag 11760 aatatttaat aagttaggga gctttctgat aatatccacc gcaaggaggc catttcgttt 11820 gcactcggtt gaagtccata cccgcctctc gttagtttca ctgacaggtg ggtccagagg 11880 cttccttcgt gtgcggttag cgagtgcacg cgtcgctcca agaaaaagca gtaggtgacc 11940 tggccacctc gttgtaggtg cgaccgatgc agacgtcccg cttgccggtg ggcccacgtt 12000 acagctgggt cccacatatc ggtgggtgcg atgagttgtt cggtgcgtag gagtagattg 12060 gatcccaacg ggtggttgcg tcgtcggcgg ccctgccgat tttattttcg atttttgaaa 12120 tgcgagagcg ggagaacggc accgttggct tggctgtgga tgccgttagc cgcaccggac 12180 tattcggccc agttccattt tggcccaact tgaaacagcg cggagcacaa gacgggggag 12240 cccatttagg cccgtaatct cagccctgta gaaagtccgt gtcgttgcga tggaccagaa 12300 agcccataaa ccttggaggc gttctctgct gggaataaaa taagttcaca ctctgtccct 12360 caactttacg tcgagtttgt ttgacatcgc taatgcccag taccagaaat cttggataca 12420 aataaggttt gatataaagt ggcacgtggt gaatggcatc gtttgaaaaa tcttcagatc 12480 gagcgggctt cacaagccgt aagtgcaagt gccaaaattt gtgaagaaaa ttacttcctt 12540 catttcacaa tgtaagttat tttagcattt tccacattca tattgatatc aataaattta 12600 gatagatata tatgtctgaa ttcgtaccgt taacgagctc tagaagcttc agtactacgc 12660 gtctcgaggc ctattattga agcatttatc agggttattg tctcatgagc ggatacatat 12720 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 12780 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 12840 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 12900 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 12960 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 13020 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 13080 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 13140 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 13200 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcacta 13260 gtgattgctc tagacactga tagtttcgga tctagatatc acatcaatcc acttgctttg 13320 aagacgtggt tggaacgtct tctttttcca cgatgttcct cgtgggtggg ggtccatctt 13380 tgggaccact gtcggtagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc 13440 atttgtagaa gccatcttcc ttttctactg tcctttcgat gaagtgacag atagctgggc 13500 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagccctct 13560 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca 13620 tgttggggat ctagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt 13680 ctttttccac gatgttcctc gtgggtgggg gtccatcttt gggaccactg tcggtagagg 13740 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtagaag ccatcttcct 13800 tttctactgt cctttcgatg aagtgacaga tagctgggca atggaatccg aggaggtttc 13860 ccgatattac cctttgttga aaagtctcaa tagccctctg gtcttctgag actgtatctt 13920 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggggatc tagatatcac 13980 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgttcctcg 14040 tgggtggggg tccatctttg ggaccactgt cggtagaggc atcttgaacg atagcctttc 14100 ctttatcgca atgatggcat ttgtagaagc catcttcctt ttctactgtc ctttcgatga 14160 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa 14220 aagtctcaat agccctctgg tcttctgaga ctgtatcttt gatattcttg gagtagacga 14280 gagtgtcgtg ctccaccatg ttggggatct agatatcaca tcaatccact tgctttgaag 14340 acgtggttgg aacgtcttct ttttccacga tgttcctcgt gggtgggggt ccatctttgg 14400 gccactgtc ggtagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt 14460 tgtagaagcc atcttccttt tctactgtcc tttcgatgaa gtgacagata gctgggcaat 14520 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctctggt 14580 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt 14640 tggggatcct ctagagtcga gaattcagta cattaaaaac gtccgcaatg tgttattaag 14700 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccagcagaga tcttcttgag 14760 cttatctgtg ctgagttggg caaggagagg aaatacacta atgttccaaa atctaaaatg 14820 attgcttatc tgttaaaatt agttttacga aagaatggac agccaaagga tgataatgca 14880 aatgcttcaa ttttggggca gaataacaaa gatgacacag agaagaaaga aaatgaggaa 14940 cagccacatc atttcagcag aagtgcaaag tctgactcat caatgtgcag agaggcccaa 15000 gctggtagca cagcggtatg ccgaaatgtt gcatgccagg ctactttgaa ctctggagat 15060 gcgtattgca agcgttgctc ttgctgcatt tgccataaat atgatgagaa caaagatcct 15120 agcttatggt tggtttgcag ctctgataca ccgtactctg gctattcatg cggcacatca 15180 tgccacctaa agtgtgctct aaagaataaa aaagctggca ttttcaagaa tgggtgcaac 15240 aagaagtcag atggtagttt ttactgtgtt tggtgtggga agatgaactg gctaatgagg 15300 tatctctttg ctattttaat tataacattc cagttattta tgtggaaaca aacacatcta 15360 aaattgtgtt ctgcatgtta tcctaattct gaagtagcat ggtgtggtac tgatccctga 15420 ccaaaactgt cctttttttt tggtagcatg cattgctcag ctttaatatc atgtttttgt 15480 gttaagcaat tcatctgata ctttatatat tgaaaatttg tgccagtttt acaatctacc 15540 cattctcatt ttggcactta caatcttggt cttgtattaa aaaatcaaga gtattcacag 15600 tgaaatttta cctctagatt tcacctctta catcattgca ggaatctgcg taagcagctg 15660 gcaattgcaa gagaatctag aagagttgat gtactgtgtg agcgtttgtc actgactcat 15720 aaaatggtga aagggtctga acgttacagg gaactggcaa acataatcaa ctcagctgtg 15780 aaaatacttg agaaagaagt tggttgtgca ttagatcaag tgtctgcaat cacggggcgg 15840 ggcatcgtga accggctttg ttgtggtgct gatgttcaaa aactttgttc atgtgcacta 15900 gaaatggtag actccacatt gagtagcacc ctggacttcg aaacaaacaa taatctggaa 15960 gcgccaggta attttgtgat agtattgact cacacaaaac tgcagttcta ctccgcaaaa 16020 ttatccctgc agaactaggc attgttatgc aaagagttat ttatgatctt ccaatgtttt 16080 gcatactacg acaatgatgt attgacattg tgtgtgaaga gattttatcc acttttgcag 16140 gtccccagcc tcaagtattt tttgtggaga ttactccatt ttctgtgctt gttgtattga 16200 agtaccagga taatatcgct gaggagattg atggttgcaa agtttggcac cgaagtgcaa 16260 acatggccaa ctatccagca gagcccactt gccacgttct tagaccaaat acaaggagtc 16320 ttttttcagg tctaagccct tctactgagt atttcttcaa ggttttgcct tttggctgtt 16380 cacagggata tggcgaatgg gaggtaaaat gctctacacg aagccttaac catggcagca 16440 gccagtgctc aacacagaat tccgagagta tgtccatcaa ggaggatttg gagcagcatc 16500 agaaaaatga gttgaatctg aagaacaagc agtggtgggg cattcaatat gactcaccct 16560 cggcaaactc gaatgagaac gatgtgtgtc ctgacctgca ccctaagcga gctaagctcg 16620 caaagttgga cggtgcaagt gacaacgacg aaagccagtt gctgccaaca tcagaagttt 16680 taccattcat gagctctaat tcttcacttt cagaagttcc aagcaaacct gactgggtta 16740 gcagcacccc agattccgcc tgcaagaacc atgttgagag gcagtatgaa tacagtgtga 16800 aggtcattag atggctggag catgaagggc acatggacaa ggatttccgc gtgaagttcc 16860 tcacatggtt tagcctgaag gcgtcggcac aggagcgacg cattgtgaac gccttcgtag 16920 atgctctggt gagtgatcct gcgagcctgg tagcacaact gattgattcg tttatggagg 16980 ttgtctgcag caaggagaag cctgctcagc cgaatggtgg gtgctgcaac ctttggcatt 17040 agaaagttcc attgccactg aggaatctca ctatcatatt tttgccaata atcatctgca 17100 tcgaaaagct gagcaattca agctatggct tattgcttta tttgatcaat ttaacactag 17160 ttgctgattg ctaggtttct gaactgtggt ggtggtggtg gtggttatgt taggtgtatg 17220 aactaagctg aagttgttga caagtgattc ctggaatgct ataaatggag gtaggtttag 17280 atgcttcaaa actggtatca tcttatccat aatgatgata tatcatttgt attttcccca 17340 aatctcgttg cttagtgatt gctgacataa tcttctttag ttacaccagt tttatgaact 17400 aggcctaagt tggttacact ctcttggagc caaactttgc ctagctaatg ctcatctgcg 17460 attgttcagt gactgtagac gagcaaccgt atgcatctag tggaaatttc gttgttggtt 17520 tgcaaga 17527 <210> 6 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> LOC_Os08g12430 F <400> 6 tgtttggtgt gggaagatga 20 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> LOC_Os08g12430 R <400> 7 gctggatagt tggccatgtt 20 <210> 8 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> ubi F <400> 8 aaccagctga ggcccaaga 19 <210> 9 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> ubi R <400> 9 acgattgatt taaccagtcc atga 24

Claims (10)

A method for increasing production of rice seeds comprising the step of inhibiting the expression of LOC_Os08g12430 gene present in genomic DNA of rice.
The method according to claim 1,
Wherein the LOC_Os08g12430 gene comprises the nucleotide sequence of SEQ ID NO: 1.
The method according to claim 1,
Wherein the inhibition of expression of the LOC_Os08g12430 gene is performed at a DNA level or an mRNA level.
The method of claim 3,
The method of inhibiting the expression of the LOC_Os08g12430 gene at the DNA level is carried out by replacing or eliminating one or more nucleotides constituting the LOC_Os08g12430 gene, which is inherently present in the genomic DNA of rice, with another nucleotide. A method of inserting another polynucleotide into the polynucleotide constituting the LOC_Os08g12430 gene; A method of frame shifting one or more nucleotides constituting the LOC_Os08g12430 gene; &Lt; / RTI &gt; and combinations thereof.
The method of claim 3,
Inhibiting the expression of the LOC_Os08g12430 gene at the RNA level inhibits the transcription of the LOC_Os08g12430 gene; a method of inactivating mRNA transcribed from LOC_Os08g12430 gene using siRNA or shRNA; &Lt; / RTI &gt; and combinations thereof.
The method according to claim 1,
Wherein the inhibition of LOC_Os08g12430 gene expression is carried out by replacing the wild type LOC_Os08g12430 gene present in the rice genomic DNA with the mutated LOC_Os08g12430 gene consisting of the nucleotide sequence of SEQ ID NO:
The method according to claim 6,
Wherein said substitution is carried out by introducing mutated LOC_Os08g12430 gene into callus of rice using Agrobacterium genus microorganism.
8. The method of claim 7,
The Agrobacterium tumefaciens LB4404 strain, the Agrobacterium tumefaciens EHA101 strain, the Agrobacterium tumefaciens EHA105 strain, and the Agrobacterium tumefaciens strain EHA105 strain, and the Agrobacterium tumefaciens EHA105 strain, &Lt; / RTI &gt; or a combination thereof.
A method for producing rice seeds with increased seed production, comprising the step of inhibiting the expression of LOC_Os08g12430 gene present in genomic DNA of rice.
A rice seed produced by the method of claim 9, wherein expression of the LOC_Os08g12430 gene is suppressed and seed yield is increased.

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