KR20160007143A - Manufacturing method of cordyceps sinensis activated rice and cordyceps sinensis activated rice by the manufacturing method - Google Patents

Abstract

The present invention relates to a method for manufacturing Cordyceps militaris mycelium activated rice, and more specifically, to a method for manufacturing Cordyceps militaris mycelium activated rice to provide rice containing a large amount of the Cordyceps militaris mycelium ingredients by culturing a thick layer of Cordyceps militaris mycelium on the surface of the rice and instilling Cordyceps militaris mycelium ingredients to the inside of the rice from the surface of the rice. To achieve this, the method for manufacturing Cordyceps militaris mycelium activated rice comprises: a slant culture step (S1) of seeding Cordyceps militaris species into a residue of the evaporation of moisture in the slanted glass tube and culturing fungal plaque, wherein a mixture of water and soybean flour, or potato juice as a main ingredient contained in a culture liquid containing fungal species growth stimulating substance is inputted into the slanted glass tube; a liquid culturing step (S2) of inputting distilled water containing germanium (Ge) into a glass container, seeding the fungal plaque cultured via the slant culture step and culturing liquefied fungal species while shaking the glass container; a solid culture step (S3) of regularly spreading steamed rice, inputting the steamed rice into a culture chamber, spraying and seeding liquid fungal species mixed with the liquified fungal species cultured via the liquid culture step and distilled water on the steamed rice to culture the Cordyceps militaris mycelium.

Description

Technical Field [0001] The present invention relates to a method of producing rice having activated mycorrhizal fungus, and to a method of producing rice using the method of the present invention, wherein the mycorrhizal mushroom-

The present invention relates to a method for producing rice which is activated by a mycelia of caterpillar fungus, and a rice which is activated by a mycelia of a caterpillar fungus produced by the method. More particularly, it relates to a method for producing a fungus- The present invention relates to a method for producing rice having activated mycelium of Chinese cabbage orchards, which contains a large amount of mycelia of Chinese cabbage, and a rice having activated mycelium of Chinese cabbage, produced by the method.

Worldwide, there are more than 300 species of caterpillar fungus, which are used for net medicines and beverages except 9, 10, and 10 kinds of fungus have.

Conventionally, the Cordyceps was inoculated into various larvae or pupae to obtain left-handed individuals. However, it has not yet succeeded in mass production in repetitive and stable conditions.

As an example of a method for producing rice cultured mycorrhizal fungus, Korean Patent Laid-Open Publication No. 2002-0086216 (Patent Document 1) discloses a phytotoxic effect of rice mushroom by cultivating mycelium of Chinese mushroom or Chinese mushroom with rice as a medium, This method has proposed a production method of functional rice which can have unique efficacy. As another example, as a typical example, a method of suspending rice bran and rice in a clean room by mixing white rice, brown rice and rice with rice, A method in which rice and mycelium are put in a box and left in a clean room have been carried out. However, according to these prior arts, after 5 to 7 days of curing from the date of culture, they were observed, However, most of them were corrupted, and also there were problems such as contamination by germs.

1. Korean Patent Publication No. 2002-0086216 (published on Nov. 18, 2002)

The object of the present invention is to provide a method for manufacturing rice and a method for producing the same, wherein the mycelia of the organism are cultivated thickly on the surface, the organisms of the organisms enter the surface of the rice from the surface of the rice, The present invention provides rice in which the mycelium of the Chinese cabbage larvae is activated.

Another object of the present invention is to provide a method for producing rice with activated mycelia of cormorant, without causing corruption or contamination by germs, and a method for producing the same.

In order to attain the above object, the present invention provides a method for producing a fermented beverage comprising 0.3 to 0.5 parts by weight of glucose, 0.3 to 0.5 parts by weight of sugar, 0.3 to 0.5 parts by weight of agar, 0.3 to 0.5 parts by weight of potassium hydroxide, 0.02 to 0.03 part by weight of phosphorus oxide (K), 0.02 to 0.03 part by weight of phosphorus pentoxide (P O 5), 0.004 to 0.008 part by weight of magnesium sulfate (MgSO 4) and 0.002 to 0.004 part by weight of manganese (Mn) After putting it in a glass tube, it is sterilized by heating at 100 ~ 130 ℃ for 20 ~ 30 minutes, and then allowed to stand for 2 ~ 3 days to evaporate water. Then, the surface of the remaining material is inoculated with a Chinese cabbage strain and cultured at 26 ~ 28 ℃ for 4 ~ 5 days A slope culturing step (S1) of forming a homogeneous surface;

A liquid culture medium containing 0.5 to 1.5 parts by weight of germanium (Ge) relative to 100 parts by weight of distilled water is placed in a flask, sterilized by heating at 100 to 130 ° C for 20 to 30 minutes, and then inoculated with the culture cultured in the slurry culture step A liquid culture step (S2) of culturing the strain at 26 to 28 DEG C for 4 to 5 days under shaking at 100 to 150 times per minute to produce a liquid specimen;

The rice is heated for 10 to 20 minutes at a temperature of 100 to 130 ° C for 20 to 30 minutes. The steamed rice is spread evenly over the sterilized netting and placed in a culture chamber. The liquid cultivation step is performed for 100 parts by weight of steamed rice 40 to 42 parts by weight of a mycelial liquid mixed with 25 to 35% by weight of liquid homogenate produced from 65 to 75% by weight of distilled water was inoculated and inoculated. Then, sterilized air and water were continuously fed into the culture chamber, For 4 to 5 days to produce a mycelia of Cordyceps; (S3); .

According to the method for producing the rice activated with the mycelia of the present invention and the method of the present invention, the mycelium of the mushroom mycelium is grown on the surface and the mushroom mycelium component penetrates into the inside from the surface of the rice It exhibits an excellent effect of containing a large amount of mycelia of Cordyceps mellifera.

In addition, it is possible to obtain rice in which high-quality mushroom mycelia of high quality, which does not cause corruption or contamination by germs, is activated.

FIG. 1 is a flowchart showing a method for producing rice activated with a mycelium of Cordyceps mellonus according to the present invention.
2 is a schematic view showing a culture chamber used in the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings which show preferred embodiments of the present invention. It should be understood, however, that there is no intention to limit the invention to the particular form disclosed, but on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. A detailed description thereof will be omitted.

In addition, for example, the Cordyceps mushroom species used in the present invention can be obtained by, for example, washing Bat moth caterpillar fungus with sterilized purified water, washing with alcohol-coated alcohol cotton having a concentration of 75%, and sterilizing a sterilized surgical knife The cut surface of the Cordyceps is scraped off and is poured with sea sand (0.1 to 1 part by weight based on 100 parts by weight of Cordyceps) with a pestle, and 120 parts by weight of purified water is added to 100 parts by weight of the ground mixture After the addition, the polished mixture was poured into a 4L reagent bottle using a pipette, and stored at a temperature of 4 ° C to prepare a Chinese cabbage stock. The bran content was 4%, and the content of copper, glucose, potassium phosphate, Magnesium and germanium were mixed and melted. When the mixture was completely dissolved by heating, the slurry was poured into a slope-like glass surface to prepare a slurry incubator. After sterilizing the slope incubator for 30 minutes at a temperature, the incubator was irradiated with ultraviolet rays for 30 minutes at a temperature of 30 cm from the slope incubator at room temperature to prepare an incubator, and the prepared caterpillar fungus was transferred to the incubator At a concentration of 11%, and the bacterium was cultivated for 8 days at a temperature of 24 ° C with an order of 130 times per minute, and the bacterium was used as the population of the bacterium. , And a dilution of 1 x 10 < ⁹ > / ml or more, but this is merely an example, and thus it is not intended to limit the method of the present invention described below.

FIG. 1 is a flow chart showing a method for producing rice activated with a mycelium of Chinese cabbage orchards according to the present invention, and FIG. 2 is a schematic view showing a culture chamber used in the present invention.

As shown in FIG. 1, the method of producing rice of the present invention, in which the mycelium of the organisms of the present invention is activated, comprises a slope cultivation step (S1), a liquid culture step (S2) and a solid culture step (S3).

The slope cultivation step (S1) is a step of cultivating a slurry containing a mixture of water and soybean powder, or a potato juice as a main material, and adding a culture medium containing a trace amount of a growth promoting substance to a slurry, The residue is then inoculated with a microorganism, and the strain is cultured.

In the slope cultivation step (S1), the culture liquid is added in an amount of 0.3 to 0.5 parts by weight of glucose, 0.3 to 0.5 parts by weight of sugar, 0.3 to 0.5 parts by weight of agar (agar) 0.02 to 0.03 part by weight of potassium (K), 0.02 to 0.03 part by weight of phosphorous pentoxide (P2O5), 0.004 to 0.008 part by weight of magnesium sulfate (MgSO4) and 0.002 to 0.004 part by weight of manganese (Mn).

In order to prevent the growth of bacteria other than the Cordycephalomyus, the glass tube in which the culture solution is introduced in the slope cultivation step (S1) is preferably sterilized by heating at 100 to 130 ° C for 20 to 30 minutes before inoculation, and then moisture is evaporated .

It is preferable that the condition for culturing the slurry in the slope culturing step (S1) is maintained at 26 to 28 DEG C for 4 to 5 days in a clean room.

In this way, the water is evaporated and the bacteria are cultured on the surface of the gel-like culture to form the bacteria.

The liquid culture step (S2) is a step in which distilled water containing germanium (Ge) is put in a glass container such as a flask, inoculated with the cultured medium in the slope cultivation step (S1), and cultured in a shaking incubator .

In the liquid culture step (S2), the mixture of distilled water and germanium may include 0.5 to 1.5 parts by weight of germanium (Ge), more preferably 1 part by weight, relative to 100 parts by weight of distilled water.

At this time, in order to prevent the growth of bacteria other than the Cordycephalosus species, it is preferable that the glass container in which the distilled water is introduced in the liquid culture step (S2) is sterilized by heating at 100 to 130 캜 for 20 to 30 minutes and then shaken in a clean room.

It is preferable that the condition for culturing the liquid mycelium in the liquid culture step (S2) is maintained at 26 to 28 DEG C for 4 to 5 days.

In this way, the liquid organism that has further propagated the Cordyceps mushroom species remains in the plast.

The solid culture step (S3) is a final step of producing the rice activated with the mycelia of caterpillar fungus, for example, using the incubation chamber shown in FIG. 2, in which the rice steamed at a high temperature and a high pressure in the kiln is spread evenly in the net 43, , And a step of inoculating a seed solution with a mixture of liquid species and distilled water cultivated through the liquid cultivation step (S2) onto steamed rice to inoculate the mycelium of the caterpillar fungus.

In order to sterilize rice, which is a kind of medium, it is preferable to add rice to water for 5 to 20 minutes and then wash it and steat it at a high pressure for 20 to 30 minutes at 100 to 130 ° C. In this case, It can be suppressed and contamination caused by decay or germs can be prevented.

In the solid culture step (S3), the mycelial liquid sprayed on the steamed rice may be a mixture of 25 to 35% by weight of the liquid species obtained in the liquid cultivation step (S2) and 65 to 75% by weight of distilled water, The amount of the fungus solution sprayed on the steamed rice is preferably such that 40 to 42 parts by weight of the fungus solution is sprayed to 100 parts by weight of the steamed rice.

The conditions for the cultivation of the mycelia of Cordyceps mushroom in the solid culture step (S3) can be maintained at 26 to 28 ° C. for 4 to 5 days by using the culture chamber of FIG.

2, the incubation chamber is provided with an air pump 20 for supplying air and moisture, and an air outlet 45 for discharging air. A heating device 42 for controlling the temperature inside the incubation chamber, And a mesh network 43 on which the steamed rice can be loaded. The air pump 20 is provided with an air filter 10 on the front thereof. The air pump 20 is connected to an air pump 20 flows into the culture case 40 through the air inlet port 41 in a state of containing a large amount of water via the water supply device 30 and flows into the air outlet port 40 provided in the culture case 40. [ (45).

More specifically, the air having passed through the air filter 10 is transferred to the water supply device 30 using the air pump 20, and air containing moisture is supplied to the culture case 30 through the water supply device 30 40 ° C. and incubated for 4 to 5 days at a temperature of 26 to 28 ° C. in a culture case 40 using a heating device 42 and a thermometer 44 provided in a culture case 40, The mycelium is cultured.

On the other hand, the rice cultivation is completed and the activated mushroom mycelium-activated rice is collected, dried in a clean and cool place, or transferred to a drying furnace and dried at a temperature of 60 to 80 캜.

Hereinafter, the present invention will be described in more detail with reference to examples of the method for producing the rice activated with the mycelia of Cordyceps melliflorus according to the present invention and the content of the active ingredients contained in the rice activated mushroom mycelium produced by the method.

[Example 1]

(a) slope culturing step;

0.4 weight part of glucose, 0.4 weight part of sugar, 0.4 weight part of agar, 0.025 weight part of potassium (K), 0.25 weight part of phosphorus pentoxide (P2O5), 0.006 weight of magnesium sulfate (MgSO4) , And 0.003 part by weight of manganese (Mn) was placed in a glass tube provided at an inclination of 15 °, and the mixture was sterilized by heating at 120 ° C for 25 minutes. The mixture was allowed to stand for 50 hours to evaporate water. Inoculated and maintained at 27.5 ° C for 100 hours to cultivate the strain.

(b) a liquid culture step;

1000 ml of a liquid culture medium containing 1 part by weight of germanium (Ge) relative to 100 parts by weight of distilled water was placed in a glass flask and sterilized by heating at 120 DEG C for 25 minutes. Then, 30 g of the obtained microflora was inoculated in the clean room And maintained at 27 DEG C for 100 hours while being shaken 120 times per minute to cultivate the liquid species.

(c) solid culture step

The rice was soaked in water for 10 minutes, washed, and steamed at 120 ° C for 20 minutes. The steamed rice was spread evenly on the sterilized netting to a thickness of 10 mm or less and placed in a culture chamber. And 41.5 parts by weight of a mycelial liquid obtained by mixing 30% by weight of liquid specimen and 70% by weight of distilled water obtained through the above step were uniformly sprayed and inoculated. The sterilized air and water were continuously fed into the culture chamber and maintained at 27 DEG C for 100 hours, Mycelia were cultured.

The components contained in the rice activated with the mycelium of Cordyceps mellifera prepared according to Example 1 were analyzed and shown in Table 1 below.

(The measurement method is 1 amino acid automatic analysis method, 2 high-speed liquid chromatography method, 3 atomic absorption light method method, 4 ICP light emission method, 5 molybdenum method, 6 vanad molybdic acid absorption method, 7 : Fluorescence spectrophotometry, 8: atmospheric pressure heat drying method).

[Table 1]

 As shown in Table 1, it can be seen that the rice activated with the mycelium of Cordyceps mushrooms produced by the present invention is rich in amino acid components.

Although the present invention has been described in connection with the preferred embodiments described above, it will be appreciated by those skilled in the art that various other modifications and variations can be made without departing from the spirit and scope of the invention, All such changes and modifications are intended to be within the scope of the appended claims.

10: air purification filter
20: Air pump
30: Water supply device
40: Culture case
41: air inlet
42: Heating device
43: Network
44: Thermometer
45: air outlet

Claims (11)

A slurry culture step (S1) in which a mixture of water and soybean powder as a main substance, a potato juice as a main ingredient, and a culture solution containing a growth promoting substance for a fungus are placed in a slanting glass tube and the residues obtained by evaporating water are inoculated with a fungus species );
A liquid culture step (S2) of adding distilled water containing germanium (Ge) into a glass container, inoculating the cultured medium through the slope culturing step, and culturing the liquid microorganism while shaking;
A solid culture step (S3) of uniformly spreading steamed rice, placing it in a culture chamber, spraying a solution of a mixture of a liquid species and a distilled water mixed with the cultured rice seedlings cultured in the liquid culture step on steamed rice to inoculate the mycelium of the caterpillar fungus;
Wherein the method comprises the steps of:
The method according to claim 1,
In the slope culturing step (S1), the culture liquid is added in an amount of 0.3 to 0.5 parts by weight of glucose, 0.3 to 0.5 parts by weight of sugar, 0.3 to 0.5 parts by weight of agar, 0.02 to 0.03 part by weight of potassium (K), 0.02 to 0.03 part by weight of phosphorous pentoxide (P2O5), 0.004 to 0.008 part by weight of magnesium sulfate (MgSO4) and 0.002 to 0.004 part by weight of manganese (Mn) A method for producing activated rice.
3. The method according to claim 1 or 2,
Wherein the glass tube in which the culture liquid is introduced in the slope culturing step (S1) is heated at 100 to 130 DEG C for 20 to 30 minutes to sterilize and then moisture is evaporated.
The method according to claim 1,
The method of claim 1, wherein 0.5 to 1.5 parts by weight of germanium (Ge) is added to 100 parts by weight of distilled water in the liquid culture step (S2).
The method according to claim 1 or 4,
Wherein the glass container in which the distilled water is introduced in the liquid culture step (S2) is sterilized by heating at 100 to 130 DEG C for 20 to 30 minutes, and then shaken in a clean room.
The method according to claim 1,
Wherein 40 to 42 parts by weight of mycelium is sprayed on 100 parts by weight of steamed rice in the solid culture step (S3).
7. The method according to claim 1 or 6,
Wherein the mycelial liquid in the solid culture step (S3) is a mixture of 25 to 35% by weight of liquid specimen and 65 to 75% by weight of distilled water.
7. The method according to claim 1 or 6,
Wherein the steamed rice is steamed at 100 to 130 ° C for 20 to 30 minutes at a high pressure by adding rice to water for 5 to 20 minutes and washing the steamed rice in the solid culture step (S3).
The method according to claim 1,
The conditions for the culture of the slurry culturing step S1 and the culturing of the liquid mycelium in the liquid culturing step S2 and the cultivation of the mycelia of the caterpillar fungus in the solid culturing step S3 are maintained for 4 to 5 days at 26 to 28 ° C Wherein the method comprises the steps of:
A slurry cultivation step of culturing a mixture of water and soybean powder, a potato juice as a main ingredient, and a culture solution containing a growth promoting substance of a fungus in a slanting glass tube, inoculating the residue of the water evaporation with a fungus species, and culturing the strain;
A liquid culture step in which distilled water containing germanium (Ge) is put in a glass container, inoculated with the cultured medium through the slope culturing step, and then incubated with shaking;
A solid culture step in which steamed rice is evenly spread and placed in a culture chamber, and a seed solution containing a mixture of liquid species and distilled water cultured in the liquid culture step is sprayed onto steamed rice to inoculate the mycelia of the caterpillar fungus;
Wherein the mycelia of rice bran are activated.
11. The method of claim 10,
0.3 to 0.5 part by weight of sugar, 0.3 to 0.5 part by weight of sugar, 0.3 to 0.5 part by weight of agar, 0.3 to 0.5 part by weight of potassium (K (K) 0.02 to 0.03 part by weight of phosphorus pentachloride, 0.02 to 0.03 part by weight of phosphorus pentoxide (P O 5), 0.004 to 0.008 part by weight of magnesium sulfate (MgSO 4) and 0.002 to 0.004 part by weight of manganese (Mn) .

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