KR20150136624A - A composition of soil treatment and a process for the decontamination of soil using the same - Google Patents
A composition of soil treatment and a process for the decontamination of soil using the same Download PDFInfo
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- KR20150136624A KR20150136624A KR1020140031971A KR20140031971A KR20150136624A KR 20150136624 A KR20150136624 A KR 20150136624A KR 1020140031971 A KR1020140031971 A KR 1020140031971A KR 20140031971 A KR20140031971 A KR 20140031971A KR 20150136624 A KR20150136624 A KR 20150136624A
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Abstract
The present invention relates to a soil treatment composition, a composition for degrading a buried carcass, or a method for the purification of a contaminated soil, in particular an animal or animal landfill. Specifically, the present invention relates to a soil treatment composition comprising at least one member selected from the group consisting of a Corynebacterium sp. Microorganism, a Corynebacterium sp. Microorganism, and a Corynebacterium sp. Microorganism concentrate, Which comprises treating a carcass landfill with a composition for degrading a buried carcass and a Corynebacterium glutamicum culture solution.
Corynebacterium glutamicum, which is a microorganism to be treated in a cadaver landfill of the present invention, is a microorganism of the GRAS (Generally Regarded As Safe) grade which is recognized as safety and is now being added to animal feeds. It is widely used industrially for producing amino acids Therefore, not only the safety is recognized, secondary pollution is not a problem, but also the carcass in the landfill can be quickly disassembled and the surrounding soil can be restored by increasing the effective amino acid content in the soil by carcass decomposition, Can be widely used in the field of environmental industry related to the treatment of buried landfill or landfill.
Description
The present invention relates to a soil treatment composition and a method for purifying contaminated soil using the same.
Due to the recent avian influenza (AI) and foot-and-mouth disease that have occurred nationwide, many livestock are being treated by landfill or burial. Although the method of disposal through landfill or burial is effective in preventing the spread of pathogens and is a relatively cost-effective treatment method, an ethical problem such as animal rights can not fundamentally treat waste livestock pollution, Or pollution of the landfill or buried land by the buried livestock and pollution of the surrounding area of these areas.
This problem has been attracting much attention due to the fact that the buried or buried livestock in existing landfill or buried land has not yet been completely decomposed and the problems caused by the odor or leachate from landfill or burial land have been investigated.
Specifically, when the livestock buried in the buried land is corrupt, ammonia nitrogen and nitrate nitrogen are emitted as livestock carcass-derived materials, and the leachate discharged from the surrounding area in the landfill causes soil and groundwater around the buried land As the concentration of nitrogen source increases, additional problems such as secondary contamination arise. Particularly, in the case of nitrate nitrogen in the nitrogen source, it is known that most of the nitrogenous organic substances are produced by oxidative decomposition of animal bodies, specifically, carcasses or excrements of animals, and they are reported as inorganic substances having harmful effects on health.
For example, according to the results of the Korea Atomic Energy Research Institute on Feb. 16, 2011, the livestock carcass-derived material was detected in four groundwater samples in the inspection area of the Songmyeonji site, Icheon city, Gyeonggi- 1 in the case of House 2, 3.817 mg / L in House 2, 1.120 mg / L in House 2, 0.597 mg / L in House of Household and 0.250 mg / L in House 3 were detected and a large amount of livestock- And the KAERI determined that there is a high possibility of contamination due to leachate when the value of the material derived from the livestock carcass is more than 1 mg / L, and groundwater in a part of the inspection area close to the livestock burial site is contaminated with the livestock- As shown in Fig.
Especially, since the area where the burial land is constructed is mainly the agricultural area, the contamination due to burial or burial of the livestock carcass is more problematic. Especially, in the agricultural area, the application of the method for removing the pollution is often restricted, The magnitude of the big problem is expected to grow even larger.
In order to solve this problem, the recently proposed method is to treat alkaline Bacillus bacteria, for example, Bacillus alcalophilus , which has been utilized as a useful microorganism. It has been reported that when the Bacillus microorganism, which is an alkaline beneficial microorganism, is treated, the decay time of the livestock is minimized and the fermentation is promoted to eliminate the odor, thereby minimizing secondary damage such as leachate contamination. In particular, the Bacillus alkalophilus is excellent in activity at an alkaline pH of from pH 8 to pH 12, and it has been reported that it acts effectively in the burnt ground treated with quicklime, unlike the probiotic or photosynthetic bacteria having activity at the existing acidic pH condition . However, it is known that the Bacillus bacterium has a disadvantage in that it can not recover the surrounding soil buried with livestock only by promoting decay of corpses and removing odors only.
The present invention provides a method for treating land contamination using a strain that exists in soil, is widely used industrially, is recognized as safe, and does not cause additional environmental pollution problems, that is, provides a method for purifying contaminated soil .
Specifically, the present invention aims to provide a soil treatment composition, and more specifically, a composition for degrading a buried carcass.
It is another object of the present invention to provide a method for purifying a contaminated soil using the microorganism.
In order to achieve the above object, the present invention provides a method for producing a corynebacterium glutamicum microorganism, which comprises culturing a microorganism of the genus Corynebacterium glutamicum, a culture of the microorganism of Corynebacterium glutamicum, As an active ingredient.
The soil treatment agent composition may be a soil treatment agent composition that is a soil treatment agent composition for soil remediation.
In addition, the Corynebacterium glutamicum microorganism may be cultured in a culture medium containing FeSO 4 , more preferably in a culture medium containing FeSO 4 for 36 hours.
In addition, the soil treatment composition may further comprise FeSO 4 .
In addition, the soil treatment composition may further comprise yeast extract.
In another aspect, the present invention relates to a method for producing a corynebacterium glutamicum microorganism, which comprises culturing at least one member selected from the group consisting of a Corynebacterium glutamicum microorganism, a culture solution of the Corynebacterium glutamicum microorganism, and a concentrate of the Corynebacterium glutamicum microorganism, The composition for decomposing the buried carcass. The Corynebacterium compartment may be cultured for one to one incubated in the culture medium containing the FeSO 4, preferably 36 hours in the culture medium containing the FeSO 4.
In addition, the composition for decomposing carcass may further comprise Yeast Extract.
In another aspect, the present invention provides a method of purifying contaminated soil comprising treating a corynebacterium glutamicum culture to a carcass landfill.
The method of purifying the contaminated soil comprises: a step of culturing the culture in which the Corynebacterium glutamicum is cultured in a culture medium containing FeSO 4 ; And treating the culture broth with a cadaver landfill.
In the culture solution preparation step, the Corynebacterium glutamicum is FeSO 4 And a yeast extract (Yeast Extract). In addition, the step of culturing the culture may be performed by culturing the Corynebacterium glutamicum in a culture medium containing FeSO 4 for 36 hours.
In addition, the step of culturing the above-mentioned culture medium may include a step of culturing Corynebacterium glutamicum in a culture medium containing FeSO 4 and a method of adding Yeast Extract to the culture medium cultured with Corynebacterium glutamicum And the like.
In addition, the step of treating the culture medium with the carcass landfill may be carried out by treating the culture medium with the carcass landfill more than once.
In the present invention, Corynebacterium glutamicum is a gram-positive aerobic bacterium, which is distributed in the epidermis and soil of animals and grows well at a weak acidic and neutral pH, and industrially produces animal feed or amino acid .
In the present invention, a domestic animal is a concept in which human beings tamed or improved wild animals, including birds called poultry in addition to mammals, which are useful for human life. For example, pigs, cows, chickens, ducks, geese, horses, donkeys, goats, dogs, cats, rabbits, turkeys, pigeons, quail and the like.
In the present invention, landfill or burial means a place buried in the ground without showing animals, organic matter, or pollutants such as livestock. Specifically, the buried land means a region buried in livestock due to infection of a livestock infectious disease do.
In the present invention, ferrous sulfate (FeSO 4 ) is widely used as a coagulant and is a compound used together with calcium hydroxide.
In the present invention, the term "culture" means a culture of a specific microorganism in a culture medium or a culture solution, and the culture may contain the specific microorganism. The culture is not limited in its formulation, and may be, for example, liquid or solid. The culture medium means a solid or a liquid containing nutrients necessary for the growth of animal cells, plant cells or bacteria.
In the present invention, the term "culture medium" means a culture inoculated with a strain in a liquid medium. The culture solution may contain a strain.
In the present invention, the concentrate of the culture liquid means the concentrate of the culture liquid, and the dry matter of the culture liquid means that the water of the culture liquid is removed.
Hereinafter, the present invention will be described in detail.
The inventors of the present invention have found that the carcass of buried livestock can be effectively decomposed in connection with the pollution treatment of livestock burial ground in which the burnt livestock has been burnt with various problems such as problems related to secondary pollution such as leachate recently, In the course of research on microorganisms which can produce useful amino acids in the process of decomposing carcass to restore the soil to the landfill, and to ensure safety and to prevent secondary pollution, the inventors of the present invention have found that, It has been confirmed that the corynebacterium glutamycin culture solution used for industrially used in the production of feed or amino acid used in the compound treatment can effectively decompose the carcass, The present inventors have completed the present invention by confirming the optimum decomposition conditions and treatment methods.
In view of the above, the present invention relates to a method for producing a corynebacterium glutamicum microorganism, which comprises culturing at least one selected from the group consisting of Corynebacterium glutamicum microorganism, the culture solution of the Corynebacterium glutamicum microorganism and the Corynebacterium glutamicum microorganism, ≪ / RTI >
The Corynebacterium glutamicum is a gram-positive aerobic bacterium, which is distributed in the epidermis and soil of animals and grows well at weakly acidic and neutral pH, industrially used for animal feed production or amino acid production, Decomposable aromatic chemicals, specifically microorganisms having an aromatic cyclic compound decomposing ability.
The Corynebacterium glutamicum may be a soil treatment composition for soil remediation from a cadaver landfill in that it can effectively decompose the carcass of buried animals.
The Corynebacterium glutamicum microorganism may be preferably cultured in a culture solution containing FeSO 4 in terms of improvement of carcass degradation efficiency.
From the viewpoint of improving the volume of the soil treatment agent, that is, the volume or the treatment efficiency with respect to the volume or weight of the microorganism, the Corynebacterium glutamicum is preferably Corynebacterium glutamicum cultured in a culture medium containing FeSO 4 , Corynebacterium glutamicum cultured in a culture medium containing FeSO 4 for 32 hours to 40 hours, more preferably Corynebacterium glutamicum cultured in a culture medium containing FeSO 4 for 34 hours to 38 hours, and even more preferably from a Corynebacterium keomil cultured for 36 hours in a culture medium that contains FeSO 4.
Furthermore, in this aspect, the Soil treatment compositions may be further comprising a FeSO 4.
In addition, the soil treatment composition may further comprise a yeast extract in terms of improving the degradation efficiency of carcass. The yeast extract may be contained at a concentration of 0.01% to 10%, preferably 0.05% to 5%, and more preferably 0.75% to 2%, based on the total weight of the composition.
The soil treatment composition comprises at least one selected from the group consisting of the active ingredient, a Corynebacterium glutamicum microorganism, a culture of the Corynebacterium glutamicum microorganism, and a concentrate of the Corynebacterium glutamicum microorganism But may further include conventional substances included in the soil treatment agent composition within a range not limiting the effects of the present invention. The soil treatment composition may contain 0.01 to 100 parts by weight or 0.01 to 99 parts by weight of the active ingredient per 100 parts by weight of the total composition.
In addition, the soil treatment composition of the present invention is characterized in that the above-mentioned effective ingredient, that is, a group consisting of a Corynebacterium glutamicum microorganism, a culture of the Corynebacterium glutamicum microorganism and a concentrate of the Corynebacterium glutamicum microorganism , A conventional soil treatment agent component, specifically, a component capable of promoting carcass decomposition or carcass decomposition, more specifically, a Bacillus alkaline phosphatase having a carcass decomposing effect, And microbial agents such as Bacillus alcalophilus .
In addition, the soil treatment composition may further contain additives conventionally used.
The present invention also relates to a pharmaceutical composition comprising at least one selected from the group consisting of a Corynebacterium glutamicum microorganism, a culture solution of the Corynebacterium glutamicum microorganism and a concentrate of the Corynebacterium glutamicum microorganism as an active ingredient To a composition for decomposing a buried carcass.
The Corynebacterium Com microorganism FeSO 4 the member decomposition efficiency is improved in a culture medium that contains, in particular, when cultured for 36 hours in a culture medium that contains FeSO 4 decomposition body unit volume or weight ratio of the composition to be treated because efficiency is most excellent, and the Corynebacterium Com Corey cultured in culture media that contains FeSO 4 Corynebacterium glutamicum Tommy compartment, preferably a culture for 32 hours to 40 hours in a culture medium that contains FeSO 4 Culturing in a culture medium containing Corynebacterium glutamicum, more preferably FeSO 4 , for 34 hours to 38 hours in a culture medium containing FeSO 4 , more preferably FeSO 4 , for 36 hours Corynebacterium glutamicum can be. Further, in this aspect, the composition for decomposing burial carcass may further comprise FeSO 4 .
In addition, the composition for decomposing burial carcass may further comprise yeast extract in terms of improvement of carcass decomposing efficiency. The yeast extract may be contained at a concentration of 0.01% to 10%, preferably 0.05% to 5%, and more preferably 0.75% to 2%, based on the total weight of the composition.
The composition for decomposing carcass comprises the above-mentioned active ingredient, a corynebacterium glutamicum microorganism, a culture of the Corynebacterium glutamicum microorganism, and a concentrate of the Corynebacterium glutamicum microorganism The present invention may further include ordinary substances contained in the composition for decomposing carcass within the range not limited to the above, and the effects of the present invention are not limited. The composition for decomposing burial carcass may contain 0.01 to 100 parts by weight or 0.01 to 99 parts by weight of the effective ingredient per 100 parts by weight of the total composition.
In addition, the composition for decomposing burial carcass of the present invention is a composition for decomposing burial carcases, which comprises the above-mentioned effective components, that is, a Corynebacterium glutamicum microorganism, a culture solution of the Corynebacterium glutamicum microorganism and a concentrate of the Corynebacterium glutamicum microorganism The present invention can be applied to a conventional soil treatment agent component, specifically, a component capable of promoting carcass decomposition or carcass decomposition, more specifically, a bacterium having a carcinogenic effect, And a microorganism such as Bacillus alcalophilus .
In addition, the composition for decomposing carcass can further contain commonly used additives.
In another aspect, the present invention relates to a method for purifying contaminated soil comprising treating a corynebacterium glutamicum culture to a carcass landfill.
The carcass that is buried in the cadaver landfill may be, but is not limited to, livestock, pigs, cattle, chickens, ducks, or goose that are specifically buried by killing.
Specifically, the method of purifying the contaminated soil comprises: a step of culturing a culture of Corynebacterium glutamicum in a culture medium containing FeSO 4 ; And treating the culture broth with a carcass landfill.
In the culture solution preparation step, the Corynebacterium glutamicum is FeSO 4 And yeast extract, preferably at least one selected from the group consisting of FeSO 4 And a yeast extract (Yeast Extract).
In addition, in the step of producing the culture, the Corynebacterium glutamicum is cultured in a culture medium containing FeSO 4 for 32 to 40 hours, preferably 34 to 38 hours, more preferably in a culture medium containing FeSO 4 For a period of time.
In addition, the culture medium manufacturing steps FeSO the Corynebacterium compartment 4, preferably FeSO 4 And a yeast extract, and a step of adding a yeast extract to a culture medium in which the Corynebacterium glutamicum is cultured. . The yeast extract may be contained at a concentration of 0.01% to 10%, preferably 0.05% to 5%, more preferably 0.75% to 2%.
The culture liquid may further contain a conventional substance contained in the soil treatment composition, carcass decomposition composition or carcass decomposition promoting composition within limits not limited to the effect of the present invention. The additional material may be added in an amount of 0 to 50 parts by weight or in an amount of 0.01 to 25 parts by weight based on 100 parts by weight of the total composition, but is not limited thereto. The further added materials include conventional soil treatment agent components, specifically microorganisms such as Bacillus alcalophilus , which have a carcass decomposing effect, such as substances capable of promoting carcass decomposition or carcass decomposition, more specifically, Which can be a microbial agent.
In addition, the culture solution may further contain additives commonly used.
The step of treating the culture medium with the carcass landfill may be carried out by treating the culture medium with a carcass landfill.
The method of treating the culture medium with a carcass landfill is characterized in that the culture solution is used in a concentration of 1 × 10 5 CFU to 1 × 10 15 CFU or 5 × 10 6 CFU to 1 × 10 10 CFU of the Corynebacterium glutamicum based on 1 g of the carcass Or 5x10 < 7 > CFU or 5x10 < 8 > CFU.
Specifically, the method for treating the culture medium with the carcass landfill may be carried out by treating the culture medium with the culture medium so that the number of treated bacteria is 7.5 to 8.5 log CFU or 7.9 to 8.3 log CFU based on 1 g of the carcass.
The method of treating the culture medium with the carcass landfill may be carried out by injecting the culture solution of the Corynebacterium glutamicum into the carcass landfill, and the method of injecting may be an in-ground injection type or a spraying type, no.
The method of treating the culture medium with the carcass landfill may be carried out by treating the culture medium twice or more. Specifically, in order to improve the degradation efficiency of the carcass, it is preferable that the culture broth is treated with 5 × 10 7 CFU or 5 × 10 8 CFU or 7.9 log CFU to 8.3 log CFU of Corynebacterium glutamicum cell mass based on 1 g of carcass The culture can be divided into four, for example, divided into four.
When the culture broth is treated separately, it may be treated at intervals of 5 to 15 days or at intervals of 7 to 12 days.
The present invention uses Corynebacterium glutamicum, a microorganism of the GRAS (Generally Regarded As Safe) grade, which is used as a conventional animal feed additive or an amino acid producing strain and is safe, In addition, it is possible to effectively and rapidly decompose the carcass in the landfill. Since the carcass decomposition can increase the effective amino acid content in the soil to restore the surrounding soil, it is possible to treat the buried land or the landfill Can be widely used in the related environmental industry field.
1 is a microphotograph of a Corynebacterium glutamicum according to an embodiment of the present invention.
FIG. 2 is a photograph showing the degradation effect of the corynebacterium glutamicum culture solution according to whether or not yeast extract was added according to an embodiment of the present invention. FIG. FIG. 2B is a photograph showing the result of treating Example 1 in which yeast extract was added and cultured. FIG.
FIG. 3 is a photograph showing a carcass decomposition effect of a Corynebacterium glutamicum culture depending on FeSO 4 addition according to an embodiment of the present invention. FIG. 3 a is a photograph of the whole process, FIG. (No cg) treated with FeSO 4 to distilled water without bacterium glutamicum. Fig. 3c is a photograph showing the result of treatment with Corynebacterium glutamicum, i.e., the yeast extract (Cg + FeSO 4 ) obtained by adding FeSO 4 to the culture solution of Corynebacterium glutamicum cultured with yeast extract added thereto, that is, 2, which shows the results.
FIG. 4A is a photograph showing a carcass decomposing effect of a Corynebacterium glutamicum culture according to a treatment method according to an embodiment of the present invention, FIG. 4A is a photograph of the entire process, FIG. 4B is a photograph of Example 2 FIG. 4C is a photograph showing the result of treatment in which the process of Example 2 was first applied, and then the process was conducted three times at intervals of 10 days, and carcass + 1 % YE means the result of treatment with yeast extract, carcass + 1% YE + C. glutamicum ( C. g ) (cyclic input) means the result of periodically treating Example 2 at intervals of 10 days, carcass + 1% YE + C. glutamicum ( C. g) ( early in) is the elapsed time after the second embodiment refers to the initial results of treatment at the same time in the, and the number described in the diagram of Figure 4a is processing the first processing solution (day), and the numbers shown in the figures of Figs. 4B and 4C mean The final measured weight of the carcass.
FIG. 5 is a photograph showing the degradation effect of the Corynebacterium glutamicum culture solution according to the culture time of the strain according to an embodiment of the present invention. FIG. 5A is a photograph of the whole process, FIG. It is a photograph of an individual body confirmed after passing. FIG No C. in 5 g Corynebacterium means a non-treated control group, and a tatami Com, C. g at 12, C. g at 24, C. g at 36 C. and 48 g at the Corey Means a treatment solution prepared by culturing four bacterial glutamicum for 12 hours, 24 hours, 36 hours and 48 hours, respectively, and then preparing a treatment solution as in Example 2. [
Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein
< Experimental Example 1> Corynebacterian Glutamicum culture
The inventors of the present invention have found that Corynebacterium < RTI ID = 0.0 > ( Corynebacterium < / RTI > glutamicum ATCC 13032) were cultured in LB broth medium.
Then, 10 g of tryptone, 5 g of Yeast Extract and 10 g of NaCl were added to and dissolved in distilled water (DW), and the resulting solution was adjusted to have a final volume of 1 L. Then, in the LB broth medium Corynebacterium glutamicum ATCC 13032, which had been cultivated at 30 ° C for 20 hours. The number of Corynebacterium glutamicum cells measured by measuring the absorbance after the culture was 7.8 log 10 CFU / mL. The cultured medium was centrifuged at 9,950 × g and 4 ° C. for 15 minutes to separate the cells. The cells were suspended in distilled water containing yeast extract at a concentration of 1% (w / w) based on the total weight of the culture medium .
In the case of the treatment solution of Example 2, 10 g of tryptone, 5 g of yeast extract and 10 g of NaCl were added to and dissolved in distilled water (DW), and the final volume was adjusted to 1 L. Then, FeSO 4 Was inoculated with the Corynebacterium glutamicum ATCC 13032 cultured in the LB broth medium and then cultured at 30 ° C for 20 hours. The cultured medium was centrifuged at 9,950 × g and 4 ° C. for 15 minutes to separate the cells. The cells were suspended in distilled water containing yeast extract at a concentration of 1% (w / w) based on the total weight of the culture medium .
In the case of the comparative example, 10 g of tryptone and 10 g of NaCl were added to and dissolved in distilled water (DW), and the final volume was adjusted to 1 L, and the Corynebacterium bacterium cultured on the LB broth medium And incubated at 30 DEG C for 20 hours. The cultured medium was centrifuged at 9,950 × g and 4 ° C. for 15 minutes to separate the cells. The cells were suspended in distilled water containing yeast extract at a concentration of 1% (w / w) based on the total weight of the culture medium .
< Experimental Example 2> Depending on culture conditions Corynebacterian Glutamic Confirm decomposition activity
The cadaver decomposing activity of the Corynebacterium glutamicum ATCC 13032 strain according to the culturing conditions was examined by treating the cadaver body with the treatment solutions of the examples and the comparative examples described above, Respectively.
2-1. Determination of activity by addition of yeast extract
In the case of degrading the carcass, the Corynebacterium glutamicum ATCC 13032 strain was expected to be able to grow without a separate source of nitrogen since nitrogen can be supplied from the protein component of the carcass. However, in the preliminary study of the present inventors, the yeast extract was affected by the decomposition activity of the degradable aromatic compound, and thus it was confirmed whether or not the yeast additives had an effect on the degradation activity.
The soil was sterilized at 121 ° C. and 1.5 atm for 3 hours, and then a portion of the sterilized soil was placed in a container sterilized under the above conditions. 22.1 g of the carcass (chicken) was placed in a container And then filled with the sterilized soil so that the carcass could be completely covered. Thereafter, 2.21 mL of each of the treatment solutions of Example 1 and Comparative Example of Experimental Example 1 was treated by widely spreading on the top of the soil covered with the above-mentioned container, and the solution was allowed to stand at room temperature, specifically at 10 to 18 DEG C for 30 days While confirming the decomposition of the dead bodies. The decomposition of the carcass was confirmed by visual observation and weight measurement. The results are shown in Fig.
As shown in FIG. 2, when the yeast extract was not added (FIG. 2A), decomposition proceeded from the outer portion of the berry when the yeast extract was added (FIG. 2B) Progress was confirmed. From the above results, it was confirmed that the yeast extract was added to improve the decomposition activity of the Corynebacterium glutamicum treated solution.
2-2. FeSO 4 Determination of activity depending on addition
Based on the results of Experimental Example 2-1, FeSO 4 influenced the decomposition activity of the degradable aromatic compound in the present invention, and thus it was confirmed whether or not FeSO 4 also affected the degradation activity Respectively.
The cadaver digestion activity test was carried out in the same manner as in Experimental Example 2-1, except that the control solution containing Corynebacterium glutamicum and yeast extract added to Corynebacterium glutamicum example 1 and was treated with Corynebacterium glutamicum treatment liquid performed by the addition of yeast extract and FeSO 4 in example 2. FIG. 3 shows the result of the confirmation after the above treatment.
As shown in FIG. 3A, when FeSO 4 was not added (FIG. 3B), when the FeSO 4 was added (FIG. 3C) It was confirmed that corruption proceeded more markedly. Specifically, as shown in FIG. 3b to FIG. 3d, in the case of the control group (FIG. 3b) in which Corynebacterium glutamicum was not added at the weight of the final dead body confirmed 28 days later, the weight slightly decreased , And it was confirmed that Corynebacterium glutamicum was the initial stage of carcass decomposition. On the other hand, it was confirmed that the carcass weight was increased by about 50% or more, and the carcass decomposition proceeded considerably. Corynebacterium glutamicum In case of Example 1 in which the yeast extract was added, the carcass weight was 32.35 g, whereas in the case of treating the treatment solution of Example 2 in which yeast extract and FeSO 4 were added to Corynebacterium glutamicum, the carcass weight was 35.09 g, The weight increased by about 10%, and the degree of carcass decomposition was more prevalent. From the above results, it was confirmed that FeSO 4 significantly affects the improvement of carcinogenic activity of the Corynebacterium glutamicum treated liquid.
2-3. Identification of activity according to treatment method of treatment liquid
Based on the results of Experimental Example 2-1 and Experimental Example 2-2, the treatment method, that is, treatment of the same treatment solution at one time and treatment of several treatments at the same time, Degradation activity of the enzyme.
The carcinogenic activity was examined in the same manner as in Experimental Example 2-1 and Experimental Example 2-2, except that the treatment was performed in the same manner as in Example 2 except that yeast extract and FeSO 4 were added to Corynebacterium glutamicum (Simultaneous dosing, initial dosing), and 0.5525 mL of the first treatment after quenching (three times in 10 days from the initial treatment) (periodic dosing). Respectively. FIG. 4 shows the results after the above-mentioned treatment.
As shown in FIG. 4A, it was confirmed that the surface of the skin was spoiled and the bones were visible, as compared with the case where the strain was not treated. Thus, it was confirmed that decomposition proceeded. After the last 31 days, In the initial simultaneous dosing, all of the skin of the cadaver was decayed, but the muscle remained undisturbed (measured weight 35.9 g and 35.6 g), whereas when periodically applied, the muscles were all degraded and only the bone tissue remained It was confirmed to be almost decomposed (measured weights of 12.25 g and 10.72 g), confirming to be more effective in the case of cyclic dosing.
2-4. Identification of activity by incubation time
Based on the results of Experimental Example 2-1 and Experimental Example 2-2, it was confirmed that the effect of culturing time of Corynebacterium glutamicum on the treatment solution was further evaluated by using Example 2, Respectively.
The carcinogenic activity was examined in the same manner as in Experimental Example 2-1 and Experimental Example 2-2, except that the treatment was performed in the same manner as in Example 2 except that yeast extract and FeSO 4 were added to Corynebacterium glutamicum , And the culturing time of Corynebacterium glutamicum added to the treatment solution in the medium supplemented with FeSO 4 was changed to 12 hours, 24 hours, 36 hours and 48 hours. FIG. 5 shows the results obtained after the initial treatment of the treatment solution containing the strains having different culture times.
As shown in FIG. 5A, it was confirmed that decomposition proceeded in all of the examples treated with the treatment solution containing the strain, as compared with the case where the strain was not treated, because the surface of the skin was spoiled and the bone was visible. After the final 14 days, the carcass weight was found to be 21.41g when cultured for 12 hours (FIG. 5b), and the carcass weight was almost increased compared with the control (20.36g) On the other hand, the weight of the carcass increased significantly from the treatment of 24 hours (33.41 g) and the weight of the carcass increased to 35.09 g for 36 hours compared with that of the treatment of 48 hours (32.32 g) The time was confirmed to be 36 hours.
From the above results, it was confirmed that Corynebacterium glutamicum was able to effectively decompose the carcass under anaerobic conditions. In particular, yeast extract and FeSO 4 were added at the time of preparing the culture, and after culturing for 36 hours, In the case of treatment with different contents, it was confirmed that the carcass decomposition effect was the best.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.
Claims (16)
Wherein the soil treatment composition is a soil treatment composition for soil remediation.
Wherein the Corynebacterium glutamicum microorganism is cultured in a culture medium containing FeSO 4 .
The Corynebacterium Com is a soil treatment agent composition of the culture for 36 hours in a culture medium that contains FeSO 4.
Soil treatment composition of the soil treatment composition further comprises a FeSO 4.
Wherein the soil treatment composition further comprises a yeast extract.
The Corynebacterium Com microorganism four Corey cultured in culture media that contains FeSO 4 tumefaciens glue Tommy Com decomposition of buried bodies composition.
Wherein the Corynebacterium glutamicum cultured in the FeSO 4 -containing culture medium is cultured in a culture medium containing FeSO 4 for 36 hours.
Wherein the composition for degrading the buried carcass further comprises a yeast extract.
The method of purifying the contaminated soil
Culturing the corynebacterium glutamicum in a culture medium containing FeSO 4 ; And
Treating the culture broth with a carcass landfill
≪ / RTI >
In the culture solution preparation step, the Corynebacterium glutamicum is FeSO 4 And a yeast extract. The method for purifying contaminated soil according to claim 1, wherein the culture is carried out in a culture medium containing yeast extract.
Wherein the culturing step is carried out by culturing the Corynebacterium glutamicum in a culture medium containing FeSO 4 for 36 hours.
The culturing step comprises culturing Corynebacterium glutamicum in a culture medium containing FeSO 4 and adding yeast extract to the culture medium cultured with Corynebacterium glutamicum. The method of claim 1, wherein the soil is contaminated.
Wherein the step of treating the culture medium with the carcass landfill is carried out by treating the culture medium with the carcass landfill more than once.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190118215A (en) | 2018-04-09 | 2019-10-18 | 이성기 | Bacterial consortium for accelerating decomposition from stamped out animal |
| KR20190141964A (en) | 2018-06-15 | 2019-12-26 | 주식회사 덕진엔지니어링 | Method and Apparatus for Restoring Soil of Burial Site for Deadstock |
| KR20200005947A (en) | 2018-07-09 | 2020-01-17 | 주식회사 덕진엔지니어링 | Method and Apparatus for High Temperature Sterilization for Deadstock and Burial Site Soil |
| CN111842477A (en) * | 2020-07-20 | 2020-10-30 | 长江大学 | Method for reinforcing phytoremediation of petroleum hydrocarbon contaminated soil by using root exudates |
| KR20220005689A (en) | 2020-07-07 | 2022-01-14 | 주식회사 엘제이바이오 | Microbial agents for promoting the decomposition of livestock carcasses including the Paraclostridium benzoelyticum KS3 strain |
| KR20220005690A (en) | 2020-07-07 | 2022-01-14 | 주식회사 엘제이바이오 | Microbial agents for promoting the decomposition of livestock carcasses including the Bacillus pseudomycoides KS2 strain |
| KR102534202B1 (en) | 2022-12-29 | 2023-05-30 | 주식회사 다산컨설턴트 | Production apparatus for livestock carcass biodegradation treatment agent |
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2014
- 2014-03-19 KR KR1020140031971A patent/KR20150136624A/en active Search and Examination
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190118215A (en) | 2018-04-09 | 2019-10-18 | 이성기 | Bacterial consortium for accelerating decomposition from stamped out animal |
| KR20190141964A (en) | 2018-06-15 | 2019-12-26 | 주식회사 덕진엔지니어링 | Method and Apparatus for Restoring Soil of Burial Site for Deadstock |
| KR20200005947A (en) | 2018-07-09 | 2020-01-17 | 주식회사 덕진엔지니어링 | Method and Apparatus for High Temperature Sterilization for Deadstock and Burial Site Soil |
| KR20220005689A (en) | 2020-07-07 | 2022-01-14 | 주식회사 엘제이바이오 | Microbial agents for promoting the decomposition of livestock carcasses including the Paraclostridium benzoelyticum KS3 strain |
| KR20220005690A (en) | 2020-07-07 | 2022-01-14 | 주식회사 엘제이바이오 | Microbial agents for promoting the decomposition of livestock carcasses including the Bacillus pseudomycoides KS2 strain |
| CN111842477A (en) * | 2020-07-20 | 2020-10-30 | 长江大学 | Method for reinforcing phytoremediation of petroleum hydrocarbon contaminated soil by using root exudates |
| KR102534202B1 (en) | 2022-12-29 | 2023-05-30 | 주식회사 다산컨설턴트 | Production apparatus for livestock carcass biodegradation treatment agent |
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