KR20190118215A - Bacterial consortium for accelerating decomposition from stamped out animal - Google Patents

Abstract

The present invention relates to a method of treating buried land of slaughtered livestock, in particular a method of treating buried land of livestock killed by foot-and-mouth disease virus, which, by administering a specific microbial cell, can effectively treat the decomposition of livestock that has been killed and purify the soil and leachate of the buried land.

Description

Bacterial consortium for accelerating decomposition from stamped out animal}

The present invention is a bacterial community that can promote the maturation of slaughtered livestock, a bacterial community that can purify leachate derived from the burial place of the slaughtered livestock, and a bacterium that can purify the soil of the burial ground of the slaughtered livestock. The present invention relates to a bacterial community capable of liquefying carcasses of communities and killed animals.

Countries dealing with livestock carcasses infected with animal disease, such as foot-and-mouth disease, bird flu, and swine cholera, which are killed or suspected of being infected, are treated in a manner appropriate to their environment, mainly anaerobic (aerobic) decomposition ( Treatment is achieved by anaerobic digestion, rendering, composting, burial, alkaline hydrolysis, lactic acid fermentation, nontraditional technologies, incineration, etc. ought. In Korea, most carcasses are treated with livestock killed by incineration or burial. Incineration is 800 ℃? It is a method of incineration in the above incinerator to reduce the volume and weight and recycle it to landfill or compost, but it is necessary to install the incinerator separately for incineration, and is reluctant to install it due to the cost burden, periodic inspections, and complaints. This is an extremely poor situation. In addition, the incineration method is not completely burned according to the type of the object and its moisture content, and the combustion of unburned substances, dust, pathogens, and severe odors are released into the atmosphere, suggesting a second environmental pollution problem. do.

In Korea, most of the livestock carcasses are processed by burial to expedite treatment at low cost.

The point of burial of these carcasses is called burial. Livestock burial sites are prohibited from being excavated and used for a certain period in accordance with the Livestock Infectious Diseases Prevention Act and the Follow-up Guidelines, but after that period, they can be used as land for their original purpose. From this point onwards, crops will be grown on most landfills.

However, excavation in the soil of the cultivated land is a common case in which the livestock remains uncorrupted, and the produce produced on or around the undamaged burial ground causes the image of local produce to be lost. In addition, livestock burial ground is a potential source of pollutants, and groundwater runs off during the dry season, and the entire groundwater of the land is likely to be seriously contaminated. It can also cause damage to the soil due to odors and residual antibiotics. In general, when carcass carcasses are buried in the ground, carcass carcasses are much slower to decompose due to lack of air circulation than when they are left on the ground. If the carcasses are slowly decomposed, odors and gases continue to occur until they are completely decomposed. There is a problem to pollute the environment. In addition, the quicklime used for the burial of livestock is mostly used for the purpose of sterilization, but it does not interfere with the decomposition rate of animal carcasses and also changes the burial area into strong alkali. There is a problem that a large number of odors and leachate occurs due to the death of microorganisms.

In order to minimize such damages, it is necessary to follow up management of livestock burial grounds, and furthermore, research and development of treatment methods for effectively destroying livestock burial grounds are required.

The inventors of the present invention have led to the present invention by studying to solve the above problems.

Republic of Korea Patent Registration 10-1324274 Republic of Korea Patent Publication No. 10-2015-0136624 Republic of Korea Patent Registration 10-1097742 Republic of Korea Patent Registration 10-1503300 Republic of Korea Patent Registration 10-1065274 Republic of Korea Patent Registration 10-1787683 Republic of Korea Patent Registration 10-1422764

One aspect of the present invention is to provide a method for biodegradation, liquefaction and soil improvement of foot-and-mouth buried livestock carcasses by microorganisms in the current state of new landfills that solve the above problems.

One aspect of the present invention is to provide a bacterial community for the maturation of the slaughtered livestock and a method for promoting the maturity of the burial land cattle using the same.

In addition, one aspect of the present invention is a method of purifying the leachate derived from the buried land of the slaughtered livestock using the bacterial community, a method of purifying the soil of the buried land of the slaughtered livestock, or killed by the foot-and-mouth disease virus To provide a way to liquefy the carcasses of livestock.

In order to achieve the above object,

One aspect of the present invention is a bacterial community for promoting maturation of slaughtered livestock,

Bacillus sp. Cy106 strain, Pseudomonas sp. Cy100 strain selected from the NBC2000 bacterial community with an accession number of KCTC 10623 BP, Pseudomonas sp. (Bacillus stearothermophilus) Cy104 strain, Bacillus sp. Cy107 strain, Pseudomonas aeruginosa Tnh strain, Bacillus sp. Cy105 strain, Pseudomonas sp. Djhc strain, Pseudomonas sp.Ntar3 strain, Serratia sp.Aeng18 strain, Serratia sp.Ntar2 strain, Aeromonas hydrophila Aeng17 strain, Cremonamona luteola ( Chryseomonas luteola Gc300 strain, Chryseomonas sp. Gc500 strain; One or more of the Bacillus cereus EBC106 strain and Pseudomonas sp. EBC107 strain selected from EBC1000 bacterial community with an accession number of KCTC 0652 BP; To provide a bacterial community for promoting lodging.

In one aspect of the invention, the bacterial community for promoting the maturity of the slaughtered livestock is Bacillus sp. Cy106 strain, Pseudomonas sp. Selected from the NBC2000 bacterial community with a deposit number of KCTC 10623 BP. Cy100 strain, Bacillus stearothermophilus Cy104 strain, Bacillus sp. Cy107 strain, Pseudomonas aeruginosa Tnh strain, Bacillus sp. Cy105 strain, Pseudomonas genus (Pseudomonas sp.) Djhc strain, Pseudomonas sp.Ntar3 strain, Serratia sp.Aeng18 strain, Serratia sp.Ntar2 strain, Aeromonas hydrophila Aeng17 Strains, Chryseomonas luteola Gc300 strains and Chryseomonas sp. Gc500 strains; Bacillus cereus EBC106 strain and Pseudomonas sp. EBC107 strain selected from EBC1000 bacterial community with an accession number of KCTC 0652 BP. To provide.

In one aspect of the invention, the slaughtered livestock provides a bacterial community for promoting maturation of the slaughtered livestock, characterized in that the livestock killed by the foot-and-mouth virus.

Another aspect of the invention, comprising the step of administering the bacterial community for promoting the housing of the slaughtered livestock to the buried land of livestock killed by the foot-and-mouth virus, to promote the maturity of the livestock killed by the foot-and-mouth virus Provide a method.

In another aspect of the present invention, characterized in that the bacterial community for promoting maturity is administered in an amount of 5 to 20 parts by weight based on 100 parts by weight of the slaughtered livestock, a method for promoting the maturity of livestock killed by foot-and-mouth disease virus. To provide.

In another aspect of the invention, the step of inserting the microbial injection pipe to the level reaching the buried carcass layer without opening the buried land; and for promoting the maturation of the slaughtered livestock through the inserted pipe It provides a method for promoting the maturation of livestock killed by foot-and-mouth virus, including administering a bacterial community.

In another aspect of the invention, the method provides a method for promoting maturation of livestock killed by foot-and-mouth disease virus, characterized in that carried out in the spring to autumn.

Another aspect of the invention, the leachate derived from the buried land of slaughtered livestock, comprising the step of administering a bacterial community for promoting the housing of the slaughtered livestock to the buried land of livestock killed by foot-and-mouth virus Provide a way to purify.

Another aspect of the invention, the method of purifying the soil of the buried land of slaughtered livestock, comprising administering to the buried land of livestock killed by foot-and-mouth virus, the bacterial community for promoting the housing of the slaughtered livestock To provide.

Another aspect of the present invention, in a method for liquefying the carcasses of livestock killed by foot-and-mouth disease virus, the method comprising the step of administering a bacterial community for promoting the maturation of the slaughtered livestock, It provides a way to liquefy carcasses that have been killed.

The method according to one aspect of the present invention may provide the best method for soil restoration without opening the foot-and-mouth disease burial site.

Method according to one aspect of the present invention can biodegradable foot-and-mouth livestock carcasses environmentally friendly.

The method according to an aspect of the present invention can effectively purify the leachate of foot-and-mouth buried land.

The method according to an aspect of the present invention has the effect of producing natural fermentation, soil improvement effect in the foot-and-mouth disease burial site.

The method according to an aspect of the present invention can prevent further groundwater contamination in foot-and-mouth buried sites.

The method according to one aspect of the present invention can also purify by connecting to the contaminated ground water.

1A to 1C are photographs of 9th microorganisms injected into a test instrument investment site.

In the present specification, when a range is described for a variable, it will be understood that the variable includes all values within the described range including the listed endpoints of the range. For example, the range "5 to 10" includes any subrange such as 6 to 10, 7 to 10, 6 to 9, 7 to 9, as well as values of 5, 6, 7, 8, 9, and 10. And any value between integers that are within the scope of the described range, such as 5.5, 6.5, 7.5, 5.5-8.5, 6.5-9, and the like. Also for example, the range of “10% to 30%” ranges from 10% to 15%, 12% to 10%, 11%, 12%, 13%, etc. as well as all integers including up to 30%. It will be understood to include any subranges such as 18%, 20% to 30%, etc., and to include any value between valid integers within the range of the stated range, such as 10.5%, 15.5%, 25.5% and the like.

Hereinafter, the present invention will be described in detail.

One aspect of the present invention is a bacterial community for promoting maturation of slaughtered livestock,

Bacillus sp. Cy106 strain, Pseudomonas sp. Cy100 strain selected from the NBC2000 bacterial community with an accession number of KCTC 10623 BP, Pseudomonas sp. (Bacillus stearothermophilus) Cy104 strain, Bacillus sp. Cy107 strain, Pseudomonas aeruginosa Tnh strain, Bacillus sp. Cy105 strain, Pseudomonas sp. Djhc strain, Pseudomonas sp.Ntar3 strain, Serratia sp.Aeng18 strain, Serratia sp.Ntar2 strain, Aeromonas hydrophila Aeng17 strain, Cremonamona luteola ( Chryseomonas luteola Gc300 strain, Chryseomonas sp. Gc500 strain; One or more of the Bacillus cereus EBC106 strain and Pseudomonas sp. EBC107 strain selected from EBC1000 bacterial community with an accession number of KCTC 0652 BP; To provide a bacterial community for promoting lodging.

In one aspect of the invention, the bacterial community for promoting the maturity of the slaughtered livestock is Bacillus sp. Cy106 strain, Pseudomonas sp. Selected from the NBC2000 bacterial community with a deposit number of KCTC 10623 BP. Cy100 strain, Bacillus stearothermophilus Cy104 strain, Bacillus sp. Cy107 strain, Pseudomonas aeruginosa Tnh strain, Bacillus sp. Cy105 strain, Pseudomonas genus (Pseudomonas sp.) Djhc strain, Pseudomonas sp.Ntar3 strain, Serratia sp.Aeng18 strain, Serratia sp.Ntar2 strain, Aeromonas hydrophila Aeng17 Strains, Chryseomonas luteola Gc300 strains and Chryseomonas sp. Gc500 strains; Bacillus cereus EBC106 strain and Pseudomonas sp. EBC107 strain selected from EBC1000 bacterial community with an accession number of KCTC 0652 BP. To provide.

In one aspect of the invention, the slaughtered livestock provides a bacterial community for promoting maturation of the slaughtered livestock, characterized in that the livestock killed by the foot-and-mouth virus.

Another aspect of the invention, comprising the step of administering the bacterial community for promoting the housing of the slaughtered livestock to the buried land of livestock killed by the foot-and-mouth virus, to promote the maturity of the livestock killed by the foot-and-mouth virus Provide a method.

In another aspect of the present invention, characterized in that the bacterial community for promoting maturity is administered in an amount of 5 to 20 parts by weight based on 100 parts by weight of the slaughtered livestock, a method for promoting the maturity of livestock killed by foot-and-mouth disease virus. To provide.

In another aspect of the invention, the step of inserting the microbial injection pipe to the level reaching the buried carcass layer without opening the buried land; and for promoting the maturation of the slaughtered livestock through the inserted pipe It provides a method for promoting the maturation of livestock killed by foot-and-mouth virus, including administering a bacterial community.

In another aspect of the invention, the method provides a method for promoting maturation of livestock killed by foot-and-mouth disease virus, characterized in that carried out in the spring to autumn.

Another aspect of the invention, the leachate derived from the buried land of slaughtered livestock, comprising the step of administering a bacterial community for promoting the housing of the slaughtered livestock to the buried land of livestock killed by foot-and-mouth virus Provide a way to purify.

Another aspect of the invention, the method of purifying the soil of the buried land of slaughtered livestock, comprising administering to the buried land of livestock killed by foot-and-mouth virus, the bacterial community for promoting the housing of the slaughtered livestock To provide.

Another aspect of the present invention, in a method for liquefying the carcasses of livestock killed by foot-and-mouth disease virus, the method comprising the step of administering a bacterial community for promoting the maturation of the slaughtered livestock, It provides a way to liquefy carcasses that have been killed.

Hereinafter, the isolation, identification and activity of the NBC2000 bacterial community and the EBC1000 bacterial community included in the foot-and-mouth disease-treated bacterial community used in the present invention will be described in detail.

NBC2000  Isolation, Identification and Activity of Bacterial Communities

One. Environmental hormones  Isolation of Degradable New Bacterial Communities

(One) Isolates bacteria that degrade PCBs, PCP, dioxin, PCE, toluene and taric acid from soils collected from Korea, Southern Europe, Oceania, etc.

1 gram of collected soil was shaken for 2 to 3 days in Luria-Bertani liquid medium (10 g of Bacterium-Tryptone, 5 g of Bakto yeast extract, 10 g of NaCl + 1 liter of demineralized water), and then 1 ml was taken to take 1 ml of Luria-Bertani agar medium ( Each colony was isolated from 10 g of Bakto-Tryptone, 5 g of Bakto yeast extract, 10 g of NaCl, 1.5% of agar, and 950 ml of demineralized water. At this time, select each colony and select the minimum liquid medium containing PCBs, PCP, PCE, toluene and taric acid (K ₂ HPO ₄ 0.065g, KH ₂ PO ₄ 0.017g, MgSO ₄ 0.1g, NaNO ₃ 0.5g + Demineralized water 1) Liters) were sequentially inoculated and shaken at 25 to 30 ^o C for at least 3 days. In addition to confirming the reduction of each harmful substance, 1 ml of shaking culture solution was taken and sequentially inoculated into Luria-Bertani agar medium and incubated at 25 to 30 ^o C for 3 to 5 days.

 Each colony separated from pure water was reinoculated into the above-described minimal liquid medium, followed by shaking culture, followed by different cultures of colonies of different shapes formed in the Luria-Bertani solid medium, and the same colonies appearing in the passage of the colonies. About 50 species were separated.

(2) Strains and morphological differences between the bacteria isolated from above were sequentially inoculated in a minimal medium containing PCBs, PCP, PCE, toluene, and taric acid in a stepwise manner, and then repeated in the same manner as in (1) above. According to the 26 species were separated.

The 26 bacteria obtained here were composed of a community named NBC2000, and each of the bacteria constituting the bacterial community was Cy100, Cy101, Cy102, Cy103, Cy104, Cy105, Cy106, Cy107, Ntar1, Ntar2, Ntar3, Gc300, Gc500, Gc501, Bs100, Aeng17, Aeng18, Sp300, Tnh, Djhc, Pcpts, EBC106, EBC107, Bs101, W24 and Nz2001.

The bacterial community NBC2000 according to the present invention was deposited on April 16, 2004 at the Genetic Resource Center of the Korea Research Institute of Bioscience and Biotechnology under accession number KCTC 10623 BP.

 2. Bacterial Community NBC2000  Establishment of Optimum Conditions for Growth

Luria-Bertani nutritional medium [10 g of bacto-tryptone, 5 g of bacto-yeast extract, 10 g of sodium chloride (NaCl) / 1 liter of demineralized water] 8, temperature 25 ~ 30 ^o C, shaking (shaking) rotation at 80 ~ 120rpm 48 ~ 96 hours incubation for optimum growth, even in subcultures grow well under the same conditions.

3. Bacterial Community NBC2000  Biochemical Identification

23 bacteria were identified by API Kits API20E, API20NE, API50CH and API50CHB purchased from bioMerieux (bioMerieux sa 69280 Marcy I'Etoile / France). To Table 5).

4. Bacterial Community NBC2000  Isolation of each bacterium

1) Strain isolation and method

Each strain consists of NBC2000 purely separated from soils such as Korea, New Zealand, Sweden, and Cyprus, all of which have mobility characteristics and can be applied to a wide variety of soils. The method of morphological characterization of individual strains in bacterial community NBC2000 is as follows.

(A) Cultivation in Luria-Bertani agar medium (10 g of Bacterium-Tryptone, 5 g of Bacterial yeast extract, 10 g of NaCl, 1.5% agar, 950 ml of demineralized water)

Cy100: After 48 hours of incubation, the surface appears as an uneven irregular yellow colony and grows to a size of 4 mm.

Cy101: Appears as translucent ivory convex colonies after 48 hours of incubation and grows to a size of 2.5 mm.

Cy102: Appears as yellow round colonies after 48 hours of incubation and grows to 2 mm in size.

Cy103: After 48 hours of incubation, it appears as an ivory round and flat colony, growing to a size of 4 mm.

Cy104: Appears as translucent ivory round colonies after 48 hours of incubation and grows to 4 mm in size.

Cy105: Appears as ivory round colonies after 48 hours of incubation and grows to a size of 2.5 mm.

Cy106: After 48 hours of incubation, it appears as a round colony with a matt white color and grows to a size of 1.5 mm.

Cy107: After 48 hours of incubation, it appears as a matt white round colony and grows to a size of 1 mm.

Ntar1: After 24 hours of incubation, it appears as a translucent yellowish, round, glossy colony, growing to a size of 1 mm.

Ntar2: After 24 hours of incubation, it appears as a round, glossy colony of beige and ivory, growing to a size of 2 mm.

Ntar3: After 48 hours of incubation, it appears as an ivory-colored round and glossy colony, growing to a size of 1.5 mm.

Gc300: After 24 hours of cultivation, it appears as an ivory round, glossy convex colony and grows to a size of 3 mm.

Gc500: After 24 hours of incubation, it appears as an ivory round, glossy convex colony, growing to a size of 1.5 mm.

Gc501: After 24 hours of cultivation, it appears as an ivory round, glossy convex colony and grows to a size of 2.5 mm.

Bs100: Appears as bright ivory round colonies after incubation for 24 hours and grows to 2 mm in size.

Aeng17: After 24 hours of incubation, it appears as round or irregular colonies of red or ivory color and grows up to 3mm in size.

Aeng18: After 24 hours of incubation, it appears as round or irregular colonies of red or ivory color and grows to 3mm in size.

Sp300: After 24 hours of incubation, it appears as round or irregular colonies of beige and brown color, growing to a size of 3 mm.

Tnh: After 40 hours of incubation, the translucent ivory surface appears as a non-uniform metallic colony and grows to a size of 4 mm. Over time, the medium changes color to indigo blue.

Djhc: After 24 hours of cultivation, the ivory is a round, smooth surface with viscous, glossy colonies that grow to a size of 3 mm.

Pcpts: After 24 hours of incubation, they appear as beige or brown round or irregular colonies and grow to a size of 3 mm.

EBC106: Appears as a flat matte colony that grows to an uneven surface after culturing for 24 hours and grows to a size of 7 mm.

EBC107: After 40 hours of incubation, the semi-transparent ivory-colored surface appears as uneven irregular colonies and grows to a size of 4 mm.

Bs101: After culturing for 24 hours, it is yellowish ivory, rounded and surrounded by a rim, and grows to a size of 5 mm. It is a gram-positive bacterium that has motility.

W24: Pale yellow, round, glossy and convex colonies that grow to a size of 1.5 mm after 48 hours of incubation. Gram-negative bacteria are motility.

Nz2001: Appears as white matte colonies after 48 h of incubation and grows to 2.5 mm. During prolonged incubation, hyphae appear at the edges of the colony, and there is motility.

(B) Maconkey solid medium [MacConkey agar: 17g peptone, Proteose peptone 3g, 10g lactose, 1.5g Bil Salts No.3, 5g sodium chloride, 13.5g agar, 0.03g neutral red, 0.001 g of crystal violet, 1 liter of demineralized water, pH7.3 ~ 7.5]

Cy100 appears in light brown small colonies,

Ntar1 is transparent light brown with uneven surface,

Ntar2 is a small beige colony,

Ntar3 is a light brown transparent brown colony,

Gc300 is a dark pink colony with beige edges,

Gc500 is most of the beige and pink colonies,

Gc501 is a dark pink colony with beige edges.

Aeng17 and Aeng18 are dark red colonies,

Sp300 is a beige colony

Tnh is a dark khaki colony,

Djhc is pink in the center of the colony and beige at the edges,

Pcpts appear in light khaki

EBC107 appears as a very pale pink colony,

Nz2001 appears as a pink beige colony,

Cy101, Cy102, Cy103, Cy104, Cy105, Cy106, Cy107, Bs100, EBC106, Bs101, W24 strains do not show colonies even after 48 hours of incubation.

(C) Desoxycholate agar [Desoxycholate agar: Protez peptone 10g, lactose 10g, sodium desoxycholate 0.5g, sodium chloride 5g, sodium citrate 2g, agar 15g, neutral red 0.03g, deionized water 1 liter, pH7.3 ... 7.5] colony color after 48 h incubation

Cy101, Cy102, Cy103, Cy104, Cy105, Cy106, Cy107, Bs100, EBC106, Bs101, 24 strains do not show colonies even after 48 hours of culture.

Cy100 is a pale pink colony that looks like a flower

Ntar1, Ntar2, and Ntar3 are transparent orange colonies,

Gc300 and Gc501 are dark pink colonies with beige edges,

Gc500 is a light reddish beige colony,

Aeng17 and Aeng18 are dark red colonies,

Sp300 is a transparent light brown colony,

Tnh is a light brown colony metallic,

Djhc is a mix of pink and beige

Pcpts are transparent brown colonies,

EBC107 appeared as a yellow colony.

Nz2001 appeared as a red beige colony.

Bs101, sulfur strain Nz2001, and oil degradation strain W24, which decompose tar, were identified as unidentified strains because it was difficult to identify by the present method.

5. NBC2000  Each of the individual strains Environmental hormone  Resolution

1) Range of environmental hormone degradation of each isolate

PCBs, dioxins, PCE, toluene and taric acid are measured by the bacterial community constituent bacteria under optimal conditions in the laboratory, and sulfur and PCP are average values measured at individual strain levels.

Pseudomonas sp. Cy100: 700 ppm PCBs;

Serratia sp. Aeng18: PCP 500 ppm, taric acid, dioxin 100 ng / kg;

Serratia sp. Ntar2: taric acid;

Pseudomonas sp. Djhc: 1000 ppm PCP, 300 ng / kg dioxin;

Pseudomonas sp. Ntar 3: taric acid;

Pseudomonas sp. EBC107: 700 ppm PCBs, 100 ppm PCP, dioxin 100 ng / kg, PCE 50,000 mg / kg;

Pseudomonas sp. Tnh: 700 ppm PCBs, 500 ppm PCP, dioxin 300 ng / kg, PCE 50,000 mg / kg, taric acid;

Aeromonas sp. Aeng17: taric acid, PCP 100 ppm, dioxin 50 ng / kg;

Pseudomonas sp. Pcpts: PCP 1,000 ppm, dioxin 500 ng / kg;

Stenotrophomonas sp. Ntar1: Taric acid;

Pseudomonas sp. Sp300: taric acid, PCP 700 ppm, dioxin 100 ng / kg;

Chryseomonas sp. Gc501: PCP 500 ppm, dioxin 100 ng / kg;

Chryseomonas sp. Gc500: PCP 500 ppm, dioxin 100 ng / kg;

Chryseomonas sp. Gc300: PCP 300 ppm, dioxin 100 ng / kg;

Brevundimonas sp. Cy101: PCBs 150 ppm;

Brebundimonas sp. Cy102: PCBs 150 ppm;

Brevundimonas sp. Cy103: 700 ppm PCBs;

Bacillus sp. Bs100: Taric acid;

Bacillus sp. Cy104: PCBs 800 ppm;

Bacillus sp. Cy105: 700 ppm PCBs;

Bacillus sp. Cy106: 1,000 ppm PCBs;

Bacillus sp. Cy107: 150 ppm PCBs;

Bacillus sp. EBC106: 700 ppm PCBs, 1,000 ppm PCP, dioxin 300 ng / kg, taric acid, PCE 50,000 mg / kg, toluene 50,000 mg / kg;

Gram positive bacterium Bs101: taric acid;

Gram negative bacteria W24: 100 ppm TPH, 100 ppm toluene;

Sulfur Strain Nz2001: Sulphur, Taric Acid, Dioxin 50 ng / kg

EBC1000  Isolation, Identification and Activity of Bacterial Communities

Hereinafter will be described in detail the isolation, identification and activity of the EBC1000 bacterial community according to the present invention.

1.Hydrolysis  toxicity Waste  Which can disassemble waste New  Isolation of Bacterial Communities

(1) separating 40 kinds of microorganisms by shaking culture of samples collected from the soil and wastewater, such as Ulsan Industrial Complex, in a liquid medium mixed with difficult-to-dissolve wastewater

1 g of soil collected from Ulsan Industrial Complex and 10 ml of waste water were collected from waste acid and waste alkaline wastewater liquid media (K ₂ HPO ₄ 0.65 g, KH ₂ PO ₄ 0.17 g, MgSO ₄ A medium formed by diluting 10% of waste acid alkaline wastes containing 0.1g, 0.5g NaNO ₃ and the non-degradable chemicals of pharmaceutical wastes and petrochemical wastes in 1l of deionized water, pH 0 ~ 14) and chlorine Liquid medium (K ₂ HPO ₄ 0.65 g, KH ₂ PO ₄ 0.17 g, MgSO ₄ 0.1 g, 0.5 g NaNO ₃ and 50 ppm contaminated phenolic compound (pentachlorophenol: PCP) were sequentially inoculated in a medium formed by dissolving 1 l of deionized water (pH 7.2) and shaken for 5 days at 20-30 ° C.

Taking 1 ml of the shake culture solution, waste acid, waste alkali solid medium (waste acid, waste alkaline liquid medium formed by adding only 1.5% of agar (agar), medium neutralization) and chlorine compound solid medium (BTB 20 in chlorine compound liquid medium) ㎎, medium formed by adding 1.5% agar) was sequentially inoculated and incubated at 20-30 ° C for 3-10 days.

Each colony was purified by re-inoculation on the same medium as above, and then shaken and cultured, and 40 kinds of useful bacteria having separate colonies of different shapes and the same colonies when subcultured were isolated.

(2) Separating 9 strong bacteria by culturing the bacteria isolated in step (1) in a medium gradually increasing the concentration of the hardly degradable waste liquid

Bacteria isolated in step (1) were used for pharmaceutical wastes and petrochemical wastes. Inoculated sequentially in each of the minimum medium was added and repeated in the same manner as in step (1), nine species were isolated according to colony type mainly viable strain at a higher concentration.

The nine bacteria obtained here were composed of one community and named EBC1000, and each bacteria constituting the bacterial community was named EBC100, 101, 103, 104, 105, 106, 107, 108 and 109, respectively.

The bacterial community EBC1000 according to the present invention was deposited internationally with the accession number KCTC 0652 BP at the Genetic Resource Center in Korea Biotechnology Research Institute on August 12, 1999.

2. Bacterial Community Of EBC1000  Establish optimal conditions for growth

PH5-8 at Luria-Bertani nutritional medium (10 g of bacto-tryptone, 5 g of bacto-yeast extract, 10 g of NaCl / l of demineralized water), temperature 25-35 ℃, shaking (shaking) 50 ~ 100rpm per minute incubation for 24 to 48 hours shows the optimum growth, even in subcultures grow well under the same conditions.

3. Bacterial Community Of EBC1000  Sympathy

EBC 100, 101, 103, 104, 105, 106, 107, 108, and 109 strains of the bacterial community EBC1000 were mixed with Gram-negative bacteria and Gram-positive bacteria. Incubated for 24 hours in LB solid medium, EBC100 is 1mm in diameter, EBC101 is 2mm in diameter, EBC103 is 2mm thick, EBC104 and EBC105 is small 0.5mm, EBC106 is 3mm EBC107 is about 1.2mm brown colony, EBC108 is about 1mm yellow colony, EBC109 is about 2mm double circle. Nine strains are aerobic and breathable strains, and EBC100, 101, 103, 106, 107, and 108 have strong viability even under strong acid (pH3 ~ 4) and strong alkali (pH9 ~ 11) conditions, and EBC104, 105, and 109 grow. This was slow. Motility is shown in EBC100, 104, 105, and 109.

In the genus of the strains constituting the bacterial community EBC1000, EBC100, 101 and 103 belong to the genus Klebsiella, EBC105 belong to the genus Providencia, EBC104 and EBC109 belong to the genus Escherichia, EBC106 belong to the genus Bacillus, EBC107 is Gram-negative bacteria and EBC108 is Gram-positive bacteria.

To summarize the characteristics of each strain as described above are shown in Table 6 to Table 8.

sample EBC 100 EBC 101 EBC 103 Gram strain
Catalase
Oxidase
Urease
Citrase utilizat.
Glucose utilizat.
VP test
Lysine decarboxylase
Ornithine decarboxylase -
+
-
+
+
+
+
+
- -







-

sample EBC 100 EBC 101 EBC 103 Inositol
Arabinos
Mannitol
Rhamnos
Glucose
Sorbitol
α-cyclodextrin
dextrin
Glycogen
Adonitol
D-Arabitol
Cellobiose
D-fructose
L-fucose
D-galactose
α-lactose
Maltose
D-rapinose
D-trehalose
Methyl-pyruvate
Citric acid
Formic acid
Malonic acid
Succinic acid
D-alanine
L-alanine
L-glutamic acid
L-serine
D, L-lactic acid
motility
D-Mannose +
+
+
+
+
+
-
+

+


+
+
+



+
-
+

-


+

+
+
+
+
+
+
+
+
+
-
+

+
+
+
+
+
+

+
+
+
+
+

-



+
+
+
+
+ +
+
+
+
+
+
-
-
-

+
+
+
+
+
-
+
+
+
+
+
+

+
+
+
+
+
+
+
+

sample EBC 104 EBC 105 EBC 109 Gram strain
ONPG
Arginine
Lysine
Ornithine
Sodium citrate
Sodium thiosulfate
Urea
Tryptophan
Indole
Pyruvate creatine sodium
Kohn Charcoal Gelatin
Glucose, potassium nitrate
Manantiol
Inositol
Rhamnos
Sucrose
Melibiose
Amigdalin
Arabinos
Oxidase
Reduction of Nitrate to Nitrite
Reduction of Nitrate to N ₂ Gas
Mobility
Oxidation of glucose
Fermentation of glucose
Antibiotic resistance

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Meanwhile, fatty acid methyl esters (FAMEs) of each strain were analyzed by gas chromatography.

Hewlett Packard series II Gas Chromatograph model 5890A (Microbial ID. Inc., Delaware, USA) was used for the analysis of FAMEs, and the separation column was 25m × 0.22mm × 0.33㎛ methylphenylsilicone. This fused capillary column (HP 19091B-102) was used.

In the gas chromatography, the carrier gas is hydrogen, the column head pressure is 10 psi, the split ratio is 100: 1, and the split vent is 50 ml / min, Septum Purge is 5 ml / min, FID hydrogen is 30 ml / min, FID nitrogen is 30 ml / min, FID air is 400 ml / min, initial temperature is 170 ° C., The program rate is 5 ° C./min, the final temperature is 270 ° C., the FID temperature is 300 ° C., the injection port is 250 ° C. and the injection volume is 2 μl.

The FAMEs graph used Microbial Identification System Software (Microbial ID, Inc., Delaware, USA) and peak identification and stagnation compared to a standard calibration mixture (Microbial ID, Inc., Delaware, USA). The time, peak area, and peak percentage were obtained.

As a result of analysis of FAMEs for each strain of EBC100, 101 and 103, the cellular fatty acids composition of EBC100 was C12: 0, C14: 0, C16: 0, C16: 1, C17: 0 cyclo, C14: 0 3OH, EBC101 is C12: 0, C14: 0, C15: 0, C16: 0, C17: 0 cyclo, C14: 0 3OH. EBC103 was found to be C14: 0, C15: 0, C16: 0, C17: 0 cyclo, C14: 0 3OH.

4. Bacterial Community EBC1000  Isolation of each bacterium

The method of separating individual strains from bacterial community EBC1000 is as follows.

EBC106 (genus Bacillus), EBC107 (Gram-negative bacteria) and EBC108 (Gram-positive bacteria) were composed of Luria-Bertan agar (10 g of Bacterium-Tryptone, 5 g of Bacterium-Yeast Extract, 10 g of NaCl, 1.5% of agar, 950 ml demineralized water) When cultured in nutrient solid medium of EBC106, the typical Bacillus-shaped thick wrinkles, EBC107 can be distinguished by the morphological features of brown colony, EBC108 is yellow colony.

Other strains include Desoxycholate agar; 10 g of Bactobacillus peptone, 10 g of Bactobacillus lactose, 1 g of sodium desoxycholate, 5 g of sodium chloride, 2 g of dipotassium, 1 g of Fe citrate, 1 g of sodium citrate, 15 g of bacto agar, neutral The colony morphology of each strain can be confirmed by diluting the bacterial community EBC1000 in 0.03 g of red red / deionized water 1-Difco manual, 1984]. After incubation in desoxycholate agar for 24 hours, EBC100, 101, and 103 showed reddish white viscosities and slight differences in size. EBC104 is red and white and viscous, EBC105 is brown, EBC106, 107 is light brown, EBC108 is colorless, and EBC109 is red (Dictionary of Microbiology and Molecular Biology, 2nd, Paul Singleton Diana Sainsbury 1987).

5. Bacterial Community Of EBC1000  characteristic

(1) Degradation rate of sodium alkylarylnaphthalene sulfonate (Tamol-SN) by bacterial community EBC1000

K ₂ HPO ₄ 0.065 g, KH ₂ PO ₄ 0.017 g, MgSO ₄ In a minimum medium (pH 7.2) made by dissolving 0.1 g and 0.5 g of NaNO _{3 in} 1 L of demineralized water, Tamol-SN was gradually increased to 500, 1000, 2000, and 4000 ppm, and the bacterial community EBC1000 was inoculated. The concentration of tamol was measured.

Tamol-SN (ppm) Degradation rate (mg / l / h) 500
1000
2000
4000 1.3
3.8
5.2
4.0

As shown in Table 9, the decomposition rate of tamol per hour was 500 ppm, and the decomposition rate was 1.3 (mg / l / h) and 4.0 (mg / l / h) at 4000 ppm.

(2) Degradation Rate of Digestive Phenol Compounds (PCP) by Bacterial Community EBC1000

After dissolving BTB 20mg in 1L of demineralized water under the minimum liquid medium conditions as shown in (1), the contaminated digestive phenolic compound (PCP) was administered as shown in Table 4, and the inoculation of bacterial community EBC1000 was inoculated with absorbance and contaminated digestive phenolic compound over time. The concentration of (PCP) was measured. The results are shown in Table 10.

Digestive Phenolic Compounds (PCP) (ppm) Degradation rate (mg / l / h) 200
500
1000
2000 0.9
6.5
5.0
4.5

Bacterial community NBC2000  And EBC1000  Foot-and-mouth disease by combination of each strain Due to virus  Body disintegration and carcass Investment  Environmental purification

Although the resolution of individual strains is limited, the treatment of bacterial communities of NBC2000 or EBC1000 as a whole or each combination of constituent strains in foot-and-mouth or carcass burial contaminated environments results in more extensive and efficient degradation. This is a synergistic effect of emergence due to mutual cooperation as a bacterial community rather than the function of individual strains constituting NBC2000 and EBC1000. No single strain can effectively destroy foot-and-mouth carcasses in a single strain, but according to the combination of each strain of NBC2000 and each strain of EBC1000 (KCTC0652 BP), the strains can be effectively complemented by complementary strains and gene transfer exchange in the community. do. In order to prove this, the inventor performed the following experiment.

Hereinafter, the present invention will be described in detail based on the following experimental examples, but the present invention is not limited thereto.

NBC2000 bacterial community  And EBC1000 bacteria community  Selection of Adaptive Strains in Extreme Environments to Control Foot-and-mouth Viruses in Selected Mixed Bacterial Communities

In the field application strains selected in the above microbial separation process, gram positive and gram negative bacteria, aerobic, anaerobic and aerobic strains that can be applied throughout, and the existing harmful compounds of PCBs, PCP, TPH, other hardly toxic substances Strains with good resolution were selected and combined.

The microbial selection combination was focused on the microbial metabolism of livestock carcasses and the adaptation of extreme environmental strains to control foot-and-mouth disease viruses. The selected strains were as follows.

Bacillus stearothermophilus cy104,

Bacillus cerus ebc106,

Bacillus sp. cy105,

Bacillus sp. cy106,

Bacillus sp. cy107,

Pseudomonas aeruginosa Tnh,

Pseudomonas sp. cy100,

Pseudomonas sp. Djhc,

Pseudomonas sp. ebc107,

Pseudomonas sp. Ntar3,

Aeromonas hydrophila Aeng17,

Serratia sp. Aeng18,

Serratia sp. Ntar2,

Chryseomonas luteola GC300,

Chryseomonas sp. GC500

The strain selected as described above was dailbiotech1200 strain, and was tested through empirical investigation of foot-and-mouth disease burial site as follows.

Foot and mouth disease Cattle burial  And biological purification of leachate

1. Overview

On-site empirical experiments were conducted to purify and restore the burial site by promoting fermentation of dead bodies and biologically friendly treatment of leachate.

2. Processing target

The A-B farmhouse used as a control and the C-E farmhouse used as a test were selected as Table 11 below.

Farm Name location Killing  Head
(pig) Remarks A 396-1, Seokcheon-ri, Baekam-myeon 4,184 Yongin / Daejo-gu B 348-1, Yongcheon-ri, Baekam-myeon 1,402 Yongin / Daejo-gu C 378-5, Yongcheon-ri, Baekam-myeon 2,154 (150 tons) dailbiotech1200 D 617-11, Yongcheon-ri, Baekam-myeon
2,489 (320 tons)
dailbiotech1200
E 217, Parkgokri, Baekam-myeon 5,627 (170 tons) dailbiotech1200

3. Processing Procedure

(1) On-site verification of livestock burial site / Promotion of carcass fermentation (carcass lysis)

(2) Determination of harmful components of leachate and subsequent treatment of leachate

(3) Burial Soil Purification

(4) groundwater purification

(5) Natural composting of field conditions of buried soil

4. Control Buried  Leachate Initial Analysis

The initial analysis of leachate water from farms A and B used as a control was conducted by the Gyeonggi-do Institute of Health and Environment, and the test results were as follows.

348-1, Yongcheon-ri, Baekam-myeon 396-1, Seokcheon-ri, Baekam-myeon (A) BOD (mg / L) 33.7 3688.0 COD (mg / L) 575.3 5945.4 T-P (mg / L) 0.114 2.252 Cu (mg / L) 0.013 ND Cr (mg / L) ND 0.02 Cd (mg / L) ND 0.006 Pb (mg / L) ND ND Mn (mg / L) 1.483 1.265 Fe (mg / L) 0.36 25.20 Zn (mg / L) 0.106 0.267 Inorganic Nitrogen (mg / L) 0 2 Total coliform group (dog / ml) 200 <30 Ammonia nitrogen (mg / L) 220.01 1503.30

5. Test Investment  Leach Resin Initial Analysis

Initial analysis of leachate water from farms C to E used as test zones was conducted by the Gyeonggi-do Institute of Health and Environment, and the test report results are shown in Table 13 below.

C D E BOD (mg / L) 23.6 23.1 46.6 COD (mg / L) 423.5 536.4 257.6 T-P (mg / L) 0.125 0.153 0.036 Cu (mg / L) 0.020 ND ND Cr (mg / L) ND ND ND Cd (mg / L) ND ND ND Pb (mg / L) ND ND ND Mn (mg / L) 1.330 0.465 22.680 Fe (mg / L) 5.48 8.28 49.43 Zn (mg / L) 0.068 0.072 0.038 Inorganic Nitrogen (mg / L) 29.2 27.2 29.3 Total coliform group (dog / ml) <30 55 100 Ammonia nitrogen (mg / L) 844.46 1110.40 28.95

6. Test  C farmhouse Investment  Leachate Indigenous Microbial Survey Results

Test District C Farmhouse Landfill Leachate, Bacillus spp., Pseudomonas spp. The results analyzed by Gyeonggi-do Institute of Health and Environment were as shown in Table 14.

Ingredient Name Analysis result (unit) Baciilus sp. 2.0 X 10 ⁴ CFU / mL Pseudomonas sp. 1.6 X 10 ⁵ CFU / mL gun Germ 4.3 X 10 ⁵ CFU / mL

As described above, the components and indigenous microorganisms of each farm leachate were analyzed before introduction of the bacterial community of the present invention. After the analysis was completed, dailbiotech 1200, a bacterial community of the present invention, was administered in the following manner.

7. Input microorganisms dailbiotech1200  Input frequency and input amount

Bacterial community was added to the C, D and E farms corresponding to the test zone as follows.

(1) Microbial input frequency

2012.2.8 (1st), 2.16 (2nd), 2.23 (3rd), 3.2 (4th), 3.12 (5th), 3.19 (6th), 3.26 (7th), 4.2 (8th), 4.9 (9th order)

(2) Total microbial input

-C Farmhouse (buying 150 tons): 3,135 liters

-D Farmhouse (buying 320 tons): 6,050 liters

-E Farmhouse (buying 170 tons): 7,930 liters

8. Test Investment Leachate  Primary analysis result

The results of the first analysis of the landfill leachate quality at 2.17 after the second injection of microorganisms from the investment site were as follows.

C D E BOD (mg / L) 157.2 377.4 102.9 COD (mg / L) 313.6 622.6 226.8 T-P (mg / L) 1.510 1.090 0.270 Cu (mg / L) 0.014 0.024 0.012 Cr (mg / L) ND ND ND Cd (mg / L) ND ND ND Pb (mg / L) ND ND ND Mn (mg / L) 0.494 1.269 10.120 Fe (mg / L) 6.13 8.02 33.36 Zn (mg / L) 0.276 0.133 0.145 Inorganic Nitrogen (mg / L) 76.6 138.3 9.1 Total coliform group (dog / ml) 74000 54000 38000 Ammonia nitrogen (mg / L) 76.16 137.95 8.70

9. Farm C, Test District Buried  Leachate Microbial Analysis

The microorganisms of the buried microorganism were analyzed by the Gyeonggi-do Institute of Health and Environment at 2.29 after the third injection of microorganisms into farm C, a test zone.

Ingredient Name Analysis result (unit) Baciilus sp. Not detected Pseudomonas sp. 6.5 X 10 ⁶ CFU / mL gun Germ 1.6 X 10 ⁷ CFU / mL

10. Test Investment Leachate  2nd analysis result

The secondary analysis of the leachate quality of the buried land at 3.2 after the 4th injection of microorganisms from the invested land was as follows.

C D E BOD (mg / L) 731.0 664.0 544.0 COD (mg / L) 793.8 800.8 973.0 T-P (mg / L) 2.296 7.117 1.387 Cu (mg / L) 0.030 0.033 0.021 Cr (mg / L) 0.01 0.02 0.02 Cd (mg / L) ND ND ND Pb (mg / L) ND ND ND Mn (mg / L) 0.066 2.326 4.066 Fe (mg / L) 25.41 40.93 43.56 Zn (mg / L) 0.423 0.367 4.832 Inorganic Nitrogen (mg / L) 61.76 78.42 118.28 Total coliform group (dog / ml) 180000 220000 280000 Ammonia nitrogen (mg / L) 61.70 78.35 118.20

11. Control  And Test Investment Leachate  3rd analysis result

The results of the third analysis of the landfill leachate quality at 4.3 after 8 microorganisms were injected from control and test sites were as follows.

Test
(C) Control
(B) Control
(A) Test
(D) Test
(E) BOD (mg / L) 830.0 36.8 9880.0 3940.0 580.5 COD (mg / L) 1027.0 349.0 13843.3 8681.3 729.9 Cu (mg / L) 0.041 ND 0.008 ND ND Cr (mg / L) ND ND ND ND 0.05 Cd (mg / L) ND ND ND ND 0.017 Pb (mg / L) ND ND ND ND ND Mn (mg / L) 0.012 0.455 1.156 4.870 39.400 Fe (mg / L) 0.69 4.14 8.33 68.44 285.10 Zn (mg / L) 0.080 0.055 0.244 0.253 1.957 Inorganic Nitrogen (mg / L) 89.93 244.21 1874.87 473.90 42.07 Total coliform group (dog / ml) 3200 56 3700 7000 1500 Ammonia nitrogen (mg / L) 89.70 244.00 1874.00 473.50 41.65 Total phosphorus (mg / L) 12.831 0.402 1.277 7.372 1.502

12. Farm C, a test zone Buried  Leachate Microbial Analysis

After the 8th microorganism was injected into farm C, test area, the result of analysis of burial microorganism through 4.2 Gyeonggi-do Institute of Health and Environment was as follows.

Ingredient Name Analysis result (unit) Baciilus sp. 2.6 X 10 ⁵ CFU / mL Pseudomonas sp. 1.8 X 10 ⁶ CFU / mL gun Germ 8.0 X 10 ⁶ CFU / mL

13. Test Buried  9th microorganism

Microorganisms were injected into the farms C, D and E, which were tested 9 times. The charged picture was as shown in Figures 1a to 1c. Figure 1a is a 9th photo of microorganisms in the C farm, Figure 1b is a 9th photo of microorganisms in the D farm, Figure 1c is a 9th microorganisms of the microorganisms in the E farmhouse.

14. Control  And Test Buried  Analysis result synthesis

The leachate quality results analyzed for the control site investment, the leachate quality results analyzed for the first, second and third analysis of the test site investment site, and the results of the comprehensive analysis of microorganisms of the C farmers in the test site investment site according to the criteria as shown in Table 20 below. Were synthesized accordingly.

Item Judgment Way BOD -High concentration organic natural fertilizer by ripening fermentation COD (Cr) -High concentration organic natural fertilizer by ripening fermentation Soluble Fe - The higher the concentration, the more the fermentation process has progressed. Soluble Mn - The higher the concentration, the more the fermentation process has progressed. NH₃-N -It is possible to confirm the fermentation progress by showing the increase and decrease as an indicator of fermentation Inorganic-N -Indicators of cadaveric fermentation, such as ammonia Coliform count (/ mL)
A total person
General Microbial Analysis -As indicator bacteria, the status of harmful strains can be checked according to the change in the coliform count.
Identification of Free Phosphorus in Tissue Cells by Corporal Maturation
-Confirm the behavior of indigenous and input microorganisms

(1) microbial analysis

Analysis of representative microorganisms such as Bacillus and Pseudomonas and other microorganisms on the C investment site of three test plots showed that indigenous microorganisms were about 10 ⁴ to 10 ⁵ , but after the first injection of dailbiotech1200, 100 Increased more than twice. After the 8th dose, it showed a tendency to decrease again, indicating that the cadaveric tissues to which the injected microorganisms approached showed good progress of biodegradation by fermentation. This means that the on-site adaptation and activity capacity of the introduced microorganism is very large.

(2) Control Buried Leachate  analysis

The two burial plots (A and B farms) are the site sites most similar to the test plots recommended by Yongin. In comparison with the initial and tertiary analyzes, in the eight major categories of leachate water, there were some changes in the inorganic nitrogen and E. coli populations, and others remained stagnant. This shows that without external purification, natural self-purification can occur or can proceed at a very slow rate.

(3) Test Buried Leachate  analysis

From the initial stage of February 8, 2012 to the 2nd of April 2, 8 out of 13 major leachate quality indicators, BOD and COD increased gradually, especially in D farms.

 The general (indigenous / injected) microbial markers and E. coli populations showed a significant increase in the first and second results compared to the initial state, but closer to the initial state in the third result. This means that microorganisms such as pathogenic harmful strains by input microorganisms are well controlled and exhibit a functional relationship between the input microorganisms and food (carcass tissue), which means that almost all of the carcasses to which the input microorganisms are accessed are decomposed.

  Soluble Fe and Mn show an increased phenomenon, and the process of cadaveric biodegradation can be known as a gradual increase in the release process from cadaveric cells.

 Ammonia nitrogen gradually decreases from the initial high concentration to the third phase, and inorganic nitrogen increases gradually from the initial to the third stage, demonstrating the microbial biodegradation of the cadaveric tissue.

 Total phosphorus, one of the main constituents of cadaveric cells, also showed higher concentration levels in the third stage than the initial state, showing the biodegradation of the cadaveric organisms by the injected microorganisms.

Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

Korea Research Institute of Bioscience and Biotechnology KCTC10623BP 20040416 Korea Research Institute of Bioscience and Biotechnology KCTC0652BP 19990811

Claims (10)

In the bacterial community for promoting the maturation of killed animals,
Bacillus sp. Cy106 strain, Pseudomonas sp. Cy100 strain selected from the NBC2000 bacterial community with an accession number of KCTC 10623 BP, Pseudomonas sp. (Bacillus stearothermophilus) Cy104 strain, Bacillus sp. Cy107 strain, Pseudomonas aeruginosa Tnh strain, Bacillus sp. Cy105 strain, Pseudomonas sp. Djhc strain, Pseudomonas sp.Ntar3 strain, Serratia sp.Aeng18 strain, Serratia sp.Ntar2 strain, Aeromonas hydrophila Aeng17 strain, Cremonamona luteola ( Chryseomonas luteola Gc300 strain, Chryseomonas sp. Gc500 strain; One or more of the Bacillus cereus EBC106 strain and Pseudomonas sp. EBC107 strain selected from EBC1000 bacterial community with an accession number of KCTC 0652 BP; Bacterial community for homeostasis.
The method of claim 1,
Bacillus sp. Cy106 strain, Pseudomonas sp. Cy100 strain selected from the NBC2000 bacterial community with an accession number of KCTC 10623 BP, Pseudomonas sp. (Bacillus stearothermophilus) Cy104 strain, Bacillus sp. Cy107 strain, Pseudomonas aeruginosa Tnh strain, Bacillus sp. Cy105 strain, Pseudomonas sp. Djhc strain, Pseudomonas sp.Ntar3 strain, Serratia sp.Aeng18 strain, Serratia sp.Ntar2 strain, Aeromonas hydrophila Aeng17 strain, Cremonamona luteola ( Chryseomonas luteola Gc300 strain and Chryseomonas sp. Gc500 strain; Bacillus cereus EBC106 strain and Pseudomonas sp. EBC107 strain selected from the EBC1000 bacterial community with an accession number of KCTC 0652 BP. .
The method of claim 1,
The killing livestock is a livestock killed by the foot-and-mouth virus, the bacterial community for promoting the maturation of the killed livestock.
A method of promoting livestock killing of livestock disposed of due to foot-and-mouth virus, the method comprising the step of administering a bacterial community for promoting the housing of the killed animal of any one of claims 1 to 3 How to promote lodging.
The method of claim 4, wherein
Wherein the bacterial community for promoting maturity is characterized in that administered to 5 to 20 parts by weight based on 100 parts by weight of the slaughtered livestock, a method for promoting the maturity of livestock killed by foot-and-mouth disease virus.
The method of claim 4, wherein
Inserting a pipe for injecting microorganisms to a level reaching a buried carcass layer without opening the investment site; and
And administering a bacterial community for promoting maturation of the killed animal, via the inserted pipe.
The method of claim 4, wherein
The method is characterized in that carried out in the spring to autumn, the method of promoting the maturation of livestock killed by foot-and-mouth disease virus.
Derived from the buried land of killed animals, comprising administering to the buried land of livestock killed by foot-and-mouth viruses, the bacterial community for promoting the lodging of the killed animals of any one of claims 1 to 3 To purify the leachate being purified.
The soil of the buried land of the slaughtered livestock, comprising the step of administering to the buried land of livestock killed by the foot-and-mouth virus, the bacterial community for promoting the lodging of the slaughtered livestock of any one of claims 1 to 3 How to cleanse.
In the method of liquefying the carcasses of livestock killed by foot-and-mouth disease virus,
The method comprises the step of liquefying the carcasses of livestock killed by foot-and-mouth disease virus, comprising the step of administering a bacterial community for promoting the maturation of the killed livestock of any one of claims 1 to 3.

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