KR20150117091A - Cosmetic composition having skin-lightening effect containing Natural component and the Fermented extract of horse placenta - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening, which is safe to skin and effective in whitening skin by using a fermented extract of Horse placenta and medical herb ingredients with reduced unique smell of Horse placenta and toxicity. The composition comprises 0.01-15 wt% active ingredients of a fermented extract of Horse placenta, a Dendropanax trifidus extract, a citron extract, a quince extract, a Juniperus chinensis extract, and a Lonicera maackii extract, with respect to the total weight of the cosmetic composition.

Description

[0001] The present invention relates to a skin whitening cosmetic composition comprising a fermented product of Matthias mellifera and a herbal medicine ingredient and a cosmetic composition having a skin-lightening effect containing a natural component and a fermented extract of horse placenta,

The present invention relates to a skin whitening cosmetic composition, and more particularly, to a skin whitening cosmetic composition which is safe to the skin and has excellent formulation stability by using a fermented product of mackerel and a herbal composition.

In general, aging of the skin, wrinkles, wrinkles, or freckles are caused by the increase in the production of collagen (melanin) due to lack of collagen. Skin aging is mainly caused by internal aging and external aging And aging is promoted mainly by external factors. Although aging of the skin is mainly caused by environmental factors such as ultraviolet rays, ultraviolet rays are essential for our life. However, when exposed for a long period of time, It causes accelerated.

The skin blackening phenomenon caused by ultraviolet rays can be divided into immediate pigment darkening and delated tannig. The primary blackening is caused by the chemical oxidation reaction of melanin, regardless of the biosynthesis of melanin, It is a phenomenon that changes to dark color and does not involve an increase of melanin. Secondary blackening is a phenomenon that melanin is newly generated by activation of tyrosinase. Melanocytes are essential cells in the human body, which plays a role in preventing ultraviolet rays, which absorb ultraviolet rays of short wavelengths, from penetrating into the dermis, and melanin is produced from the melanocytes of melanocytes. And the appearance of the skin color is determined by the presence of a large number of melanin pigments.

Therefore, in order to maintain or improve skin whitening, the inhibition of melanin production and the applied components are applied to cosmetic ingredients. For example, a light scattering agent or a light blocking agent is used for ultraviolet shielding, which is a main cause of melanin production, A component related to inhibition of activity, a component that decolorizes melanin to reduce it, and the like are applied to cosmetic ingredients.

Recently, herbal medicines have been reported to have excellent moisturizing power, anti-aging function and antioxidant effect, and the use of herbal medicines has become prominent in the field of cosmetics. Thus, many studies have been made on cosmetic compositions using herbal medicinal extracts. For example, Discloses a cosmetic composition which is excellent in antioxidation, anti-aging, whitening, wrinkle-improving or moisturizing effect, which contains a mixed extract of herbal medicines composed of herbal medicine, No. 10-1251040 discloses a herbal medicine extract consisting of 100 parts by weight of a herbal medicine material containing marjoram, citrus peel, and acacia fruit, and 500 parts by weight of a herbal extract. 1 to 70% by weight, and the cosmetic base as the remaining amount But also, as the remaining amount, discloses a snail mucus filtrate 1 to hwangchil whitening cosmetic composition using a wood extract to 5% by weight, characterized in that it further comprises.

In addition, since the extracts from the horse placenta exhibit not only the action of preventing pigmentation but also the whitening action (Mallick Set el., Pigment Cell Res. Feb 18 (1), 25-33, 2005) And a composition for skin whitening comprising, as an active ingredient, a nanoliposome containing an extract of Maehu and Matthew halves in Korean Patent Laid-Open Publication No. 10-2010-0104144 as a prior art. However, the horse placenta extract has disadvantages that it is easily discolored due to its unique odor and instability, and irritation occurs to the skin when used at a high concentration.

The present invention has completed the present invention by producing a skin whitening cosmetic which is safe to the skin and has excellent stability of the formulation by combining the herbal ingredients together with the elimination of the odor of the mate- rial semi-fermented product and the reduction of the toxicity.

The present invention aims at providing a skin whitening cosmetic composition containing a horse placenta fermented product and a herbal ingredient, and more particularly, to a horse placenta fermented product having a reduced odor and toxicity And to provide a skin whitening cosmetic composition which is very safe on the skin and has excellent whitening effect by using herbal ingredients.

As a solution to achieve the object of the present invention, the skin whitening cosmetic composition using the mathematical semi-fermented product and the herbal composition comprises 60 to 80% by weight of the maturity semi-fermented product, the extract of Huacaraceae, citron extract, And 4 to 8% by weight of wood extracts are each constituted by 0.01 to 15% by weight based on the total weight of the cosmetic composition.

The horse placenta is composed of nucleic acid components such as essential amino acids, RNA and DNA, antioxidant enzymes such as SOD (Super Oxide Dismutase), Hyaluronic acid, antioxidant, immunocompetent factor (cytocine), placental peptide, Growth factors such as insulin-like growth factor, epidermal growth factor (EGFS) and unique senescent cell activating factor (SCAFS), and cytokines such as fatigue recovery, Immune enhancement, etc.

The horse placenta fermented product according to the present invention is prepared by inoculating the placenta with at least one microorganism selected from microorganisms of lactic acid bacteria and fermenting the fermented product at a fermentation temperature of 38 ° C for 10 days, The fermentation process is carried out for about 3 months. After the fermentation process is finished, the fermented material is obtained by filtering.

In the case of the above-mentioned mathematical half-fermented product, if the condition is set incorrectly during the fermentation process, there is a phenomenon of spoilage accompanied with a bad odor. In case of normal normal fermentation, the pH of the fermentation broth is stably maintained at about 3 to 4 Lt; / RTI >

Examples of the lactic acid bacteria according to the present invention include Lactobacillus sp. Lactobacillus sp., Lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus brevis, Lactobacillus acidophilus, Pediococcus pentosaceus, Pediococcus cerevisiae, Lactobacillus spp., Lactobacillus spp., Lactobacillus spp., Lactobacillus spp. One from Lactococcus lactis, Leuconostoc citreum, Leuconostoc mesenteroides, Bifidobacterium bifidum, and Lactobacillus helveticus. One is from Lactococcus lactis, Leuconostoc citreum, Leuconostoc mesenteroides, Bifidobacterium bifidum and Lactobacillus helveticus. Or more.

The antimicrobial activity of the mate- rial fermented product according to the present invention varies according to the kind of the microorganism to be used. However, in the present invention, Lactobacillus (Lactobacillus sp. plantarum, Lactobacillus delbrueckii, Lactobacillus acidophilus, and Lactobacillus helveticus are preferred. Among them, Lactobacillus helveticus is preferred, and Lactobacillus spp. It is more preferable to use it.

In addition, the present invention is a plant widely known as a medicinal plant such as Huangchu, Yuzu, Juza, Jiangsu, Jiangsu, and Nanbu trees, and the Huangchu tree extract is obtained by extracting well dried Huangchu tree with hot water at 80 ° C for 3 to 5 hours, Next, the filtrate is used, and citron, quince, juniper, and necropolis are extracted by the same method.

It is preferable that the extracts of each of the above-mentioned woody spruce, citron, citrus fruit, jasmine, and nematode have a solid content ratio of 0.1 to 2.0 wt% based on the weight ratio of the extract. If the solid content exceeds 2.0 wt% There are difficulties in industrialization.

The skin whitening cosmetic composition using the matthews half fermented product and herbal medicine component extract of the present invention is a non-irritating, safe, non-toxic and non-toxic skin by removing the characteristic odor by using the fermented product of the martial arts. It shows excellent whitening effect by the effect of strengthening antioxidant and immunity strengthening function.

Fig. 1 is a picture of the inhibition of melanin formation by a mixture of a fermented product of Matthias mellifera and a herbal medicine ingredient extract.
FIG. 2 is a graph showing a decrease in the pre- and post-use staining of Formulation Example 3 containing a mixture of the maturity-half fermented product and the herbal medicine component extract.

 Hereinafter, the present invention will be described in detail with reference to Examples and Test Examples. However, the present invention is not limited by the following examples.

≪ Example 1 >

One). Manufacture of fermented mate

The matthews were stored frozen immediately after harvesting and thawed during the fermentation process. 200 g of sugar and 300 g of oligosaccharide were added to 2 kg of freeze-crushed matte semi-pulverized product, and the mixture was inoculated with 3% (w / v) of Lactobacillus helveticus and fermented at a fermentation temperature of 38 ° C for 10 days .

Thereafter, the fermentation product was aged at room temperature for about 3 months. After completion of the fermentation process, the fermented product was filtered to obtain a fermented product. The obtained fermented product had a solid content of 1.2 wt% 3.5.

2). Production of herbal medicine ingredient extract

1 Kg of well-dried Hwangryu-jinhwa was extracted with hot water of 80 캜 for 4 hours, cooled to room temperature, and filtered to obtain an extract. The solids content of the extract obtained was 1.5% by weight based on the weight of the extract and the pH was found to be 6

In addition, citron, quince, juniper, and nunchaku were extracted by the same method to obtain respective extracts.

3). Manufacture of a mixture (active ingredient) of a fermented mate-rial and herbal extracts

A mixture (active ingredient) of the matthews fermented product according to the present invention and the herbal medicine component extract (active ingredient) was prepared by mixing the above-prepared matthews semi-fermented product with the herbal medicine component extract. The compounding ratio was 75 wt% Extracts, citron extracts, mosses extracts, jungle extracts, and nematode extracts were each adjusted to 5% by weight.

The skin macerating effect was tested using the mixture (active ingredient) of the horse-placenta fermented product as an active ingredient according to the present invention prepared in Example 1 and the herbal medicine ingredient extract as follows.

≪ Test Example 1 > In vitro tyrosinase activity inhibition test

Tyrosinase is an enzyme involved in determining the most important initial rate in the melanin biosynthetic pathway in the human body. Since many whitening ingredients have an action mechanism that inhibits this enzyme, this test shows the activity of tyrosinase enzyme in vitro The sample is diluted to a suitable concentration range to inhibit tyrosinase activity by dissolving in ethanol or an appropriate solvent. The sample solution is a sample solution (minimum of 5 concentration ranges).

Add 220 μl of 0.1 M phosphate buffer (pH 6.5), 20 μl of sample solution and 20 μl of mushroom tyrosinase (1500-2000 U / ml) (or human tyrosinase) solution in the test tube in this order. Add 40 μl of 1.5 mM tyrosine solution to this solution and incubate at 37 ° C for 10 to 15 minutes.

The absorbance is measured at 490 nm using an ELISA reader. Add 0.1 M phosphate buffer (pH 6.5) instead of the sample solution to the blank. The concentration (IC50) of the sample when the active inhibition rate is 50% is calculated using an appropriate program. The results are shown in Table 1 below. It is possible to experiment with different scales depending on the experimental conditions. As the positive control group, arbutin or ethyl ascorbyl ether can be used.

(%) = 100- (b-b '/ a-a') 100

A: absorbance of the blank after the reaction, and b: absorbance of the sample after the reaction. a ', b': absorbance measured by replacing with buffer instead of tyrosine

IC50 tyrosinase inhibitory concentration (ppm) Remarks Vitamin C 55 Mixture of Fermented Maceous and Fermented Extracts 310

As shown in Table 1, the IC50 value of the mixture of the fermentation product of mata-fermented product and the herbal medicine ingredient is higher than that of vitamin C, whereas the vitamin C is a pure single compound. On the other hand, It can be said that the tyrosinase inhibitory activity is very excellent.

Test Example 2 Melanin production inhibition test

In this <Test Example 2>, B16-F1 melanoma cells secreting melanin black pigment were cultured as a cell strain derived from a mouse, and a mixture of the matal-fermented product of the present invention and the herbal medicine ingredient extract was treated to obtain melanin black pigment Compared with the control group.

The strain number of the B16-F1 melanoma cell used in the present invention was ATCC6323. For the inhibition of melanin biosynthesis of B16 melanoma cells, B16 melanoma cells were suspended in a suspension containing 10% fetal bovine serum at a concentration of 5 x 10 ⁴ cells / ml, and 5 ml of the suspension cells were suspended in a tissue culture flask , 0.134% of a black sample was added thereto, and the mixture was incubated at 37 ° C for 4 days in a CO ₂ incubator having 5% carbon dioxide and 95% air conditions. The degree of melanin production was firstly discriminated by the color of the culture medium, Washed with buffer and trypsinized to separate the cells from the flask.

Cells were collected in tubes, washed with phosphate buffer, centrifuged, and the amount of melanin precipitated at the bottom of the tube was compared with the known kojic acid. The evaluation method was to visually observe the degree of color change depending on the degree of secretion of black melanin pigment when no treatment was performed during cell culture and when each sample was treated.

The results are shown in FIG. 1, and it was concluded from the results shown in FIG. 1 that the mixture of the mash fermented product of the present invention and the herbal composition extract effectively inhibited melanin pigment formation.

&Lt; Example 2 > Preparation of Cosmetic Formulation

The following <Formulation Examples> are given to aid understanding of the present invention, and the present invention is not limited to the following Formulation Examples.

a). Formulation Example 1: Skin Toner INCI Name Quantity (g) Remarks Propanediol 7.00 Dipotassium glycyrrhizate 0.10 Allantoin 0.10 PEG-60 hydrogenated castor oil 0.20 Dipropyleneglycol 5.00 Glycerin 4.00 Sodium carbomer 0.15 Phenoxyethanol 0.30 Mixture of Fermented Maceous and Fermented Extracts 0.10 Hexanediol 2.00 Water to 100.00

b). Formulation Example 2: Serum INCI Name Quantity (g) Remarks Butandiol 10.00 Dipotassium glycyrrhizate 0.10 Allantoin 0.10 PEG-40 Hydrogenated Castor Oil 0.10 Adenosine 0.04 Acrylates / C10-30 alkyl acrylate crosspolymer 0.15 Xanthan gum 0.05 Phenoxyethanol 0.30 Methylparaben 0.20 PEG-40 hydrogenated castor oil, PPG-26-Buteth-26, butylene glycol, glycerine, Copper Tripeptide-1, Phenoxyethanol, Methylparaben, Butylparaben, Ethylparaben, Isobutylparaben, Propylparaben,

0.01 Lysine, Histidine, Arginine, Aspartic acid, Threonine, Serine, Glutamic acid, Proline, Glycine, Alanine, Valine, Methionine, Isoleucine, Leucine, Tyrosine, Phenylalanine, Cysteine

0.01 Acetyl hexapeptide-8 0.10 Mixture of Fermented Maceous and Fermented Extracts 1.00 Water to 100.00

c). Formulation Example 3: Nourishing cream INCI Name amount (%) Remarks Glycerin 5.00 Allantoin 0.10 Dipotassium glycyrrhizate 0.10 Propanediol 5.00 Sparassis crispa extract 1.50 Lactobacillus / Soymilk ferment extract 1.00 Hydrogenated lecithin 1.00 Mixture of Fermented Maceous and Fermented Extracts 5.00 Xanthan gum 0.10 Bentonite 0.40 Cetearyl alcohol 1.50 Glyceryl stearate, PEG-100 stearate 1.00 Simmondsia chinensis (Jojoba) seed oil 1.00 Macadamia ternifolia seed oil 1.00 Butyrospermum parkii (Shea butter) 1.00 Cetearyl olivate, Sorbitan olivate 3.00 Tocopheryl acetate 0.40 Dimethicone 1.00 Caprylhydroxamic acid, Caprylyl glycol, Glycerin 0.50 Phenoxyethanol 0.30 Water to 100.00

d). Formulation 4: Eye Cream INCI Name amount (%) Remarks Glycerin 5.00 Pentylene glycol 3.50 Glucose, Sorbitol, Citric acid 0.10 Butylene glycol 3.00 Water, Sodium hyaluronate 7.00 Water, btylene glycol, carbomer, sodium stearate, hydrolyzed wheat protein, zinc gluconate, atelocollagen, Sodium Chondroitin Sulfate
0.50 Disodium EDTA 0.03 Petrolatum 4.00 Mineral oil, Lanolin alcohol 2.00 Ceresin 3.00 Butyrospermum parkii (Shea butter) 4.00 Lauryl PEG-9 polydimethylsiloxyethyl dimethicone 2.00 Phenyl trimethicone 4.00 Limnanthes alba (Meadowfoam) seed oil 2.00 Vitis vinifera (Grape) seed oil 1.00 Tocopheryl acetate 0.50 Silica 0.50 Arachidyl alcohol, Behenyl alcohol, Arachidyl glucoside 2.00 PEG-8 Dilaurate 1.00 Mixture of Fermented Maceous and Fermented Extracts 15.00 Phenoxyethanol 0.30 Caprylhydroxamic acid, Caprylyl glycol, Glycerin 0.50 Fragrance 0.10 Water to 100.00

The cosmetic composition of the present invention can be blended with an ordinary ingredient to be incorporated in a cosmetic composition for general skin. The oil component content of the cosmetic composition according to the present invention is selected in consideration of enhancement of emulsifying ability and economical efficiency, and at least one oil selected from vegetable oil, mineral oil, silicone oil and synthetic oil can be used . In addition, 0.1 to 5% by weight of a surfactant, a higher alcohol and the like may be added to reinforce the emulsifying ability. As such surfactants, conventional surfactants such as nonionic surfactants, phospholipids and the like can be used. As the higher alcohols, alcohols having 12 to 20 carbon atoms can be used singly or in combination.

In addition, in the cosmetic composition, 0.001 to 5% by weight of one or more kinds of thickening agents such as carbomer, xanthan gum, bentonite and the like may be further added in order to control the viscosity or hardness of the water phase. In addition, the composition of the present invention may further contain a pharmaceutical ingredient such as higher fatty acids and vitamins, and components used in conventional cosmetics such as an ultraviolet screening agent, an antioxidant, a preservative, a flavoring agent, a coloring agent, and a pH adjusting agent. The cosmetic composition of the present invention is a product which is absorbed through the skin, and is a softening product (skin), a convergent lotion, a nutritional lotion (lotion), a cleanser, a nutritional cream, a massage cream, an essence, a gel, a pack, a powder, Emulsion, spray, or serum, and may contain various conventional carriers and additives suitable for each of these formulations and known in the art.

While the present invention has been described with reference to certain preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiments.

Fermented product of the present invention can be added in an amount of 0.01 to 15% by weight based on the total weight of the cosmetic composition in consideration of the stability and palatability of the formulation, and when the amount is less than 0.01% by weight, the efficacy / Fermented product and the herbal medicine component extract are contained in an amount of 0.1 to 15 wt% based on the total weight of the cosmetic composition, and since the stability of the formulation is low at 15 wt% or more, By weight.

&Lt; Test Example 3 > Stability test of cosmetic formulation

The formulation examples prepared in Example 2 were subjected to a long-term preservation test (temperature: 25 ± 2 ° C. / relative humidity: 60 ± 5%) and an accelerated test (temperature: 40 ± 2 ° C. / relative humidity: 75 ± 5 All tests were performed for 6 months for the long-term storage test, 3 months for the accelerated test, and 10 days for the severe test. The physicochemical changes such as separation, electrophoresis and discoloration of more than 1 month were not observed and it was confirmed that the formulation had a very stable formulation.

Test Example 4: Verification of Skin Whitening Effect in Formulation Example

The nutritional cream of Formulation Example 3 prepared in Example 2 was used for six weeks in a stigmatized woman, and skin staining changes were observed. The results are shown in [Fig. 2], and as shown in Fig. 2, skin stains are reduced and skin color is generally brightened.

Claims (5)

Fermented product of Matthews semi-fermented product, an active ingredient comprising an extract of Huacaraceae, citron extract, mosquito extract, juniper extract and granulosa extract, in an amount of 0.01 to 15% by weight based on the total weight of the cosmetic composition, Composition for skin whitening cosmetic composition. The fermented milk according to claim 1, wherein the active ingredient is composed of 60 to 80% by weight of a fermented macening product, 4 to 8% by weight of an extract of Huaculi, Yuza, Morinda, A skin whitening cosmetic composition using water and herbal ingredients. The method according to claim 2, wherein the active ingredient is composed of 75% by weight of a fermented mate- rial fermented product, 5% by weight of Houttuynia cordata extract, 5% by weight of citron extract, 5% by weight of corn extract, 5% Characterized in that it is a skin whitening cosmetic composition using a fermented product of Matthias and herbal ingredients. The method according to any one of claims 1 to 3, wherein the semi-fermented product of Matthias comprises a matte bacterium selected from the group consisting of Lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus acidophilus ) And Lactobacillus helveticus, and fermenting the fermented product for 3 months. The skin whitening cosmetic composition according to claim 1, wherein the fermented product is aged for 3 months. The cosmetic composition according to claim 4, wherein the cosmetic composition is at least one selected from the group consisting of softening longevity (skin), convergent lotion, nutritional lotion (lotion), cleanser, nutritional cream, massage cream, essence, gel, pack, powder, ointment, Wherein the composition is a spray, or a formulation of a serum.

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