KR20150117088A - Cosmetic composition having anti-wrinkle effect containing Natural component and the Fermented extract of horse placenta - Google Patents

Abstract

The present invention relates to a cosmetic composition for reducing skin wrinkles, which is safe to skin and effective in reducing wrinkles by using a fermented extract of Horse placenta and medical herb ingredients with reduced unique smell of Horse placenta and toxicity. The composition comprises 0.01-15 wt% active ingredients of a fermented extract of Horse placenta, a Dendropanax trifidus extract, a citron extract, a quince extract, a Juniperus chinensis extract, and a Lonicera maackii extract, with respect to the total weight of the cosmetic composition.

Description

[0001] The present invention relates to a cosmetic composition having an anti-wrinkle effect and a fermented extract of horse placenta,

The present invention relates to a cosmetic composition for improving skin wrinkles, and more particularly, to a cosmetic composition suitable for skin wrinkle improvement management which is safe to skin and has excellent formulation stability using a semi-fermented product and a herbal composition.

In general, skin wrinkles are known to be produced by various factors such as moisture content of skin, collagen content, immunity to external environment, and especially, 70% Of the collagen fibers is reduced by aging, or the collagen fibers are deformed by the external environment, so that a lot of wrinkles are generated and deepened on the skin.

Conventionally, in order to improve the wrinkles of the skin, the composition has been provided as a cosmetic composition for suppressing transdermal water evaporation, a collagen for providing skin elasticity, and the like. In recent years, herbal medicines have been used for moisturizing, anti- , And especially in the field of cosmetics, the use of herbal medicines has become prominent, and there have been many studies on cosmetic compositions using herbal extracts.

In recent years, extracts from horse placenta have been shown to exhibit not only the action of preventing pigmentation but also the whitening action (Mallick Set el., Pigment Cell Res. Feb 18 (1), 25-33, 2005) It has been found that it is effective for suppressing and improving wrinkles and is used for materials such as cosmetics and foods. However, it has disadvantages in that it is easily discolored due to peculiar odor and instability in the extract of Matthew Semen, and irritation appears on skin when used at high concentration.

Background Art [0002] Prior arts related to a cosmetic composition for improving skin wrinkles containing a herbal composition as a cosmetic composition for improving skin wrinkles are variously known. For example, in Korean Patent Registration No. 10-1125225, A cosmetic composition for improving skin wrinkles containing an ethyl acetate fraction obtained by dissolving an extract of C1 to C4 lower alcohols or a mixed solvent thereof in water in the order of n-hexane and ethyl acetate in order; And a mixed herbal medicinal herb extract is prepared by solvent extraction of mixed herbal medicines of Baekbuja, Baekjji, Youngryungbyeo, Hyangbuja, Baekbokryeong and Gamsohyeong in Korean Registered Patent Publication No. 10-1142541. Then, A mixture for improving skin wrinkles characterized by being obtained by treating with a hydrolytic enzyme and a pectin hydrolase (Korean Patent Registration No. 10-1257641) discloses a method of extracting a phytotoxic extract of a Chinese herbal medicine, which is a method of using Chinese medicine, A first step (S101) of preparing a herbal medicine ingredient for whitening skin and improving wrinkles by blending and selecting any one of the herbal ingredients of the present invention; After filtering the liquid material by the various steps, the extracted extract is adjusted to such a degree that it becomes viscous, and then the purified oil is added thereto and reheated to 70 ~ 80 ° C for 0.5 ~ 1 hour to gelate And a method for manufacturing a herbal composition cosmetic composition for skin whitening and wrinkle improvement.

In addition, Korean Patent Registration No. 10-0987302 discloses a composition comprising: i) 0.2 to 0.6 parts by weight of a protease selected from papain, protease, peptidase, pepsin, or a mixture thereof, and 30 to 40 parts by weight of water, Adding 100 parts by weight of the enzyme to the first proteolytic reaction at 60 to 70 ° C; ii) 0.05 to 0.2 parts by weight of the protease is further added to the reaction product of i) to perform a second proteolytic reaction at 60 to 70 ° C; and iii) the reaction product of ii) has a moisture content of 1 to 3 1.0 to 1.5 parts by weight of activated charcoal in weight% and then removing the odor and color under a high purity nitrogen or vacuum pressure of 0.1 to 0.5 atm. By weight based on the total weight of the composition.

The present invention has completed the present invention by preparing a cosmetic for skin wrinkle which is safe for skin and has excellent stability of formulation, by combining the herbal ingredients together with elimination of odor and peculiar odor by the fermented mackerel.

The present invention aims at providing a cosmetic composition for improving skin wrinkles containing a horse placenta fermented product and a herbal medicine component, and more particularly, to a cosmetic composition for improving the skin wrinkle, which comprises a mace pan specific odor and a horse placenta ) It is an object of the present invention to provide a cosmetic composition for improving wrinkles which is long-lasting and highly safe while exhibiting a wrinkle-improving effect of skin in a short time by using a fermented product and a herbal medicine ingredient.

As a solution to achieve the object of the present invention, a cosmetic composition for improving skin wrinkles using a semi-fermented product and a herbal composition comprises 60 to 80% by weight of a mathematical half fermented product, an extract of Huachilmum, citron extract, And 4 to 8% by weight of each of the extracts of Panax ginseng extract is contained in an amount of 0.01 to 15% by weight based on the total weight of the cosmetic composition.

The horse placenta is composed of nucleic acid components such as essential amino acids, RNA and DNA, antioxidant enzymes such as superoxide dismutase (SOD), hyaluronic acid, antioxidants, immunocompromising factors (cytocine), placental peptides, Growth factors such as insulin-like growth factor, epidermal growth factor (EGFS) and unique senescent cell activating factor (SCAFS), and cytokines such as fatigue recovery, Immune enhancement, etc.

The horse placenta fermented product according to the present invention is prepared by inoculating the placenta with at least one microorganism selected from microorganisms of lactic acid bacteria and fermenting the fermented product at a fermentation temperature of 38 ° C for 10 days, The fermentation process is carried out for about 3 months. After the fermentation process is finished, the fermented material is obtained by filtering.

In the case of the above-mentioned mathematical half-fermented product, if the condition is set incorrectly during the fermentation process, there is a phenomenon of spoilage accompanied with a bad odor. In case of normal normal fermentation, the pH of the fermentation broth is stably maintained at about 3 to 4 Lt; / RTI >

Examples of the lactic acid bacteria according to the present invention include Lactobacillus sp. Lactobacillus sp., Lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus brevis, Lactobacillus acidophilus, Pediococcus pentosaceus, Pediococcus cerevisiae, Lactobacillus spp., Lactobacillus spp., Lactobacillus spp., Lactobacillus spp. One from Lactococcus lactis, Leuconostoc citreum, Leuconostoc mesenteroides, Bifidobacterium bifidum, and Lactobacillus helveticus. One is from Lactococcus lactis, Leuconostoc citreum, Leuconostoc mesenteroides, Bifidobacterium bifidum and Lactobacillus helveticus. Or more.

The antimicrobial activity of the mate- rial fermented product according to the present invention varies according to the kind of the microorganism to be used. However, in the present invention, Lactobacillus (Lactobacillus sp. plantarum, Lactobacillus delbrueckii, Lactobacillus acidophilus, and Lactobacillus helveticus are preferred. Among them, Lactobacillus helveticus is preferred, and Lactobacillus spp. It is more preferable to use it.

In addition, the present invention is a plant widely known as a medicinal plant such as Huangchu, Yuza, Quercus, Jiangsu, Jiangsu, and Ngongbu. The extract of Huchulchiai is extracted with hot water at 80 ° C for 3 to 5 hours, , And citron, quince, juniper, and necropolis are extracted by the same method.

It is preferable that the extracts of each of the above-mentioned woody spruce, citron, citrus fruit, jasmine, and nematode have a solid content ratio of 0.1 to 2.0 wt% based on the weight ratio of the extract. If the solid content exceeds 2.0 wt% There are difficulties in industrialization.

The cosmetic composition for improving skin wrinkles using the matal half-fermented product and the herbal medicine component of the present invention has no specific odor and toxicity due to the use of the fermented product of the martial arts, so that it is non-irritating to the skin and safe, And exhibits an effect of improving wrinkles, exhibiting a short period of time, and exhibiting a long lasting effect because of its excellent cell activity through cell proliferation, the effect of enhancing collagen synthesis, and enhancing immunity.

Brief Description of the Drawings Fig. 1 is a chart showing collagen synthesis performance test results of a mixture of a fermented product of Matthias mellon and a herbal medicine ingredient extract; Fig.
FIG. 2 is a graph showing collagenase inhibition experiment results of a mixture of a fermented product of Matthias mellifera and herbal medicine ingredient extract. FIG.
FIG. 3 is a picture showing the result of the skin wrinkle-improving effect after using the formulation containing the mixture of the maturity-half-fermented product and the herbal medicine component extract.

Hereinafter, the present invention will be described in detail with reference to Examples and Test Examples. However, the present invention is not limited by the following examples.

≪ Example 1 >

One). Manufacture of fermented mate

Matsutake is kept in a frozen state immediately after harvesting and thawed during the fermentation process. 200 g of sugar and 300 g of oligosaccharide are added to 2 kg of the matte semi-pulverized product and mixed with 3% w of Lactobacillus helveticus / v) and fermented at a fermentation temperature of 38 占 폚 for 10 days.

Thereafter, the fermentation product was aged at room temperature for about 3 months. After completion of the fermentation process, the fermented product was filtered to obtain a fermented product. The obtained fermented product had a solid content of 1.2 wt% 3.5.

2). Production of herbal medicine ingredient extract

1 Kg of well-dried Hwangchujang was extracted with hot water at 80 캜 for 4 hours, cooled to room temperature and filtered to obtain an extract. The solid content of the extract was 1.5% by weight based on the weight of the extract and the pH was 6 .

In the same manner as above, citron, quince, juniper, and nematode were also extracted to obtain respective extracts.

3). Manufacture of a mixture (active ingredient) of a fermented mate-rial and herbal extracts

A mixture (active ingredient) of the matthews fermented product according to the present invention and the herbal medicine component extract (active ingredient) was prepared by mixing the above-prepared matthews semi-fermented product with the herbal medicine component extract. The compounding ratio was 75 wt% 5% by weight of extracts, citron extract, mosquito extract, juniper extract, and nematode extract.

The wrinkle-reducing effect was tested using the mixture (active ingredient) of the fermented mate- rial fermented product according to the present invention prepared in Example 1 and the herbal medicine ingredient extract as shown in the following <Test Example>.

<Test Example 1> Intracellular collagen production test

The test method of <Test Example 1> is to compare the degree of increase in intracellular collagen production of the sample with the blank test solution when the fibroblast culture is performed.

Human dermal fibroblasts were inoculated on the bottom of the culture dish and cultured in DMEM (Dulbecco ' s Modified) supplemented with penicillin (100 IU / ml), streptomycin (100 占 퐂 / ml), 10% fetal bovine serum Eagle's Medium) medium and incubated at 37 ° C in an incubator containing 5% carbon dioxide. Concentrations of the test solutions were selected by preliminary experiments using MTT assay to show no cytotoxicity.

When the test substance was not dissolved in the DMEM medium, it was dissolved in a suitable solvent such as ethanol, and TGF-β was used as a positive control substance . Fibroblasts are seeded in a 48-well plate at a density of 5 × 10 ⁴ cells per well and cultured for 24 hours under cell culture conditions. Discard the medium, wash with 10% PBS (phosphate buffered saline), add the test solution and fresh medium, and incubate for 24 hours. Take the culture medium and measure the amount of collagen.

The amount of collagen measured was corrected by the total amount of collagen obtained by the Lowry assay. The standard solution was diluted with water to 0, 10, 20, 40, 80, 160, 320 and 640 ng / , And the amount of collagen was quantitated by ELISA using a Procollagen type I peptide EIA kit (Takara Biomedical Co.). Add 100 μl of the Antibody-PoD conjugate solution to the wells, add 20 μl of the diluted culture solution and 1/5 of the standard solution, and incubate at 37 ° C for 3 hours. After removing the culture medium from the wells, wash with 400 ㎕ of phosphate buffer (PBS) 4 times. Add 100 ㎕ of color development reagent, incubate at room temperature for 15 minutes, add 100 ㎕ of 1 N sulfuric acid and measure with an ELISA reader at 450nm.

As shown in FIG. 1, the collagen synthesis rate of the mixture of the matthias semi-fermented product and the herbal composition extract was similar to that of retinol, whereas the control (adenosine) In the range of 0.1 ~ 0.001% concentration, the fermented mate - rial fermentation increased by 35% ~ 25%, indicating that the fermented mate - rial was excellent for cell regeneration.

&Lt; Test Example 2 > Inhibition test for intracellular collagenase activity

The test method of <Test Example 2> is to compare the degree of inhibition of intracellular collagenase production by the sample when the fibroblast is cultured.

Select a cell line from the intracellular collagen synthesis test and prepare a sample solution after cell culture. Fibroblasts are seeded in a 48-well plate at a density of 5 × 10 ⁴ cells per well and cultured for 24 hours under cell culture conditions.

The medium is discarded and washed with 10% phosphate buffered saline (PBS). The test solution and fresh medium are then added and cultured for 48 hours. Take the culture medium and measure the amount of collagenase. The amount of collagenase measured was corrected to the total amount of protein determined by the Lowry method.

The results are shown in FIG. 2 and are higher than that of retinol at a concentration of 25 ppb as shown in FIG. 2, but retinol is a pure compound, and the mixture of the fermentation product of mata- It can be seen that the collagenase inhibiting activity is very excellent.

&Lt; Example 2 > Preparation of Cosmetic Formulation

The following <Formulation Examples> are for the purpose of helping understanding of the present invention, and the present invention is not limited to the Formulation Examples.

a). Formulation Example 1: Serum INCI Name Quantity (g) Remarks Butandiol 10.00 Dipotassium glycyrrhizate 0.10 Allantoin 0.10 PEG-40 Hydrogenated Castor Oil 0.10 Adenosine 0.04 Acrylates / C10-30 alkyl acrylate crosspolymer 0.15 Xanthan gum 0.05 Phenoxyethanol 0.30 Methylparaben 0.20 PEG-40 hydrogenated castor oil, PPG-26-Buteth-26, butylene glycol, glycerine, Copper Tripeptide-1, Phenoxyethanol, Methylparaben, Butylparaben, Ethylparaben, Isobutylparaben, Propylparaben,

0.01 Lysine, Histidine, Arginine, Aspartic acid, Threonine, Serine, Glutamic acid, Proline, Glycine, Alanine, Valine, Methionine, Isoleucine, Leucine, Tyrosine, Phenylalanine, Cysteine

0.01 Acetyl hexapeptide-8 0.10 Mixture of Fermented Maceous and Fermented Extracts 1.00 Water to 100.00

b). Formulation Example 2: Nourishing cream INCI Name amount (%) Remarks Glycerin 5.00 Allantoin 0.10 Dipotassium glycyrrhizate 0.10 Propanediol 5.00 Sparassis crispa extract 1.50 Lactobacillus / Soymilk ferment extract 1.00 Hydrogenated lecithin 1.00 Mixture of Fermented Maceous and Fermented Extracts 5.00 Xanthan gum 0.10 Bentonite 0.40 Cetearyl alcohol 1.50 Glyceryl stearate, PEG-100 stearate 1.00 Simmondsia chinensis (Jojoba) seed oil 1.00 Macadamia ternifolia seed oil 1.00 Butyrospermum parkii (Shea butter) 1.00 Cetearyl olivate, Sorbitan olivate 3.00 Tocopheryl acetate 0.40 Dimethicone 1.00 Caprylhydroxamic acid, Caprylyl glycol, Glycerin 0.50 Phenoxyethanol 0.30 Water to 100.00

c). Formulation Example 3: Skin Toner INCI Name Quantity (g) Remarks Propanediol 7.00 Dipotassium glycyrrhizate 0.10 Allantoin 0.10 PEG-60 hydrogenated castor oil 0.20 Dipropyleneglycol 5.00 Glycerin 4.00 Sodium carbomer 0.15 Phenoxyethanol 0.30 Mixture of Fermented Maceous and Fermented Extracts 0.10 Hexanediol 2.00 Water to 100.00

d). Formulation Example 4: Eye cream INCI Name amount (%) Remarks Glycerin 5.00 Pentylene glycol 3.50 Glucose, Sorbitol, Citric acid 0.10 Butylene glycol 3.00 Water, Sodium hyaluronate 7.00 Water, btylene glycol, carbomer, sodium stearate, hydrolyzed wheat protein, zinc gluconate, atelocollagen, Sodium Chondroitin Sulfate
0.50 Disodium EDTA 0.03 Petrolatum 4.00 Mineral oil, Lanolin alcohol 2.00 Ceresin 3.00 Butyrospermum parkii (Shea butter) 4.00 Lauryl PEG-9 polydimethylsiloxyethyl dimethicone 2.00 Phenyl trimethicone 4.00 Limnanthes alba (Meadowfoam) seed oil 2.00 Vitis vinifera (Grape) seed oil 1.00 Tocopheryl acetate 0.50 Silica 0.50 Arachidyl alcohol, Behenyl alcohol, Arachidyl glucoside 2.00 PEG-8 Dilaurate 1.00 Mixture of Fermented Maceous and Fermented Extracts 15.00 Phenoxyethanol 0.30 Caprylhydroxamic acid, Caprylyl glycol, Glycerin 0.50 Fragrance 0.10 Water to 100.00

The cosmetic composition of the present invention can be blended with an ordinary ingredient to be incorporated in a cosmetic composition for general skin. The oil component content of the cosmetic composition according to the present invention is selected in consideration of enhancement of emulsifying ability and economical efficiency, and at least one oil selected from vegetable oil, mineral oil, silicone oil and synthetic oil can be used . In addition, 0.1 to 5% by weight of a surfactant, a higher alcohol and the like may be added to reinforce the emulsifying ability. As such surfactants, conventional surfactants such as nonionic surfactants, phospholipids and the like can be used. As the higher alcohols, alcohols having 12 to 20 carbon atoms can be used singly or in combination.

In addition, in the cosmetic composition, 0.001 to 5% by weight of one or more kinds of thickening agents such as carbomer, xanthan gum, bentonite and the like may be further added in order to control the viscosity or hardness of the water phase. In addition, the composition of the present invention may further contain a pharmaceutical ingredient such as higher fatty acids and vitamins, and components used in conventional cosmetics such as an ultraviolet screening agent, an antioxidant, a preservative, a flavoring agent, a coloring agent, and a pH adjusting agent. The cosmetic composition of the present invention is a product which is absorbed through the skin, and is a softening product (skin), a convergent lotion, a nutritional lotion (lotion), a cleanser, a nutritional cream, a massage cream, an essence, a gel, a pack, a powder, Emulsion, spray, or serum, and may contain various conventional carriers and additives suitable for each of these formulations and known in the art.

While the present invention has been described with reference to certain preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiments.

Fermented product of the present invention can be added in an amount of 0.01 to 15.00% by weight in consideration of the stability and palatability of the formulation. If the amount is less than 0.01% by weight, the effect / It is difficult to industrially apply it because the stability of the formulation is low and the possibility of being changed by the base is high. Thus, it is preferable that the mixture of the semi-fermented product of mackerel and the extract of crude drug components is added in the range of 0.1 to 15% by weight based on the total weight of the cosmetic composition Do.

&Lt; Test Example 3 > Stability test of cosmetic formulation

The formulation examples prepared in Example 2 were subjected to a long-term preservation test (temperature: 25 ± 2 ° C. / relative humidity: 60 ± 5%) and an accelerated test (temperature: 40 ± 2 ° C. / relative humidity: 75 ± 5 All tests were performed for 6 months for the long-term storage test, 3 months for the accelerated test, and 10 days for the severe test. The physicochemical changes such as separation, electrophoresis and discoloration of more than 1 month were not observed and it was confirmed that the formulation had a very stable formulation.

&Lt; Test Example 4 >

The eye cream of Formulation Example 4 prepared in Example 2 was applied to a woman with skin wrinkles for 8 weeks, and the change in skin wrinkle depth was confirmed by replica method. As a result, as shown in FIG. 3, both the depth and height of the wrinkles were reduced, the skin was smoothed, and the wrinkle reduction effect was about 17%.

Claims (5)

Fermented product of Matthews semi-fermented product, an active ingredient comprising an extract of Huacaraceae, citron extract, mosquito extract, juniper extract and granulosa extract, in an amount of 0.01 to 15% by weight based on the total weight of the cosmetic composition, Composition for improving skin wrinkles. [Claim 4] The method according to claim 1, wherein the active ingredient comprises 60 to 80% by weight of a fermented macening product, and 4 to 8% by weight of an extract of Huachilin, Yuza, Quercus, A cosmetic composition for improving skin wrinkles using a fermented product and a herbal medicine ingredient. The method according to claim 2, wherein the active ingredient is composed of 75% by weight of a fermented mate- rial fermented product, 5% by weight of Houttuynia cordata extract, 5% by weight of citron extract, 5% by weight of corn extract, 5% Characterized in that the cosmetic composition for improving the wrinkles of the skin using the fermented material and the herbal composition. The method according to any one of claims 1 to 3, wherein the semi-fermented product of Matthias comprises a matte bacterium selected from the group consisting of Lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus acidophilus ) And Lactobacillus helveticus, and then fermenting the fermented product for 3 months. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the fermented product is aged for 3 months. [Claim 4] The cosmetic composition according to claim 4, wherein the cosmetic composition is at least one selected from the group consisting of a softening longevity (skin), a convergent lotion, a nutritional lotion (lotion), a cleanser, a nutritive cream, a massage cream, an essence, a gel, a pack, a powder, Or a cosmetic composition, wherein the cosmetic composition for improving skin wrinkles comprises a fermented product of martens and a herbal composition.

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