KR20150096237A - Composition containing fermented material of pear and ginger for immune activity - Google Patents

Abstract

The present invention relates to a composition including a mixed fermentation product of pear and ginger for enhancing immunity. Particularly, the mixed fermentation product, which is an active ingredient of the present invention, is proven to have an excellent ability of enhancing immunity activity when compared to a case of treating a sample merely with a pear extract or a ginger extract as a testing result of measuring the weight of kidney and thymus gland, change of the number of white blood cells, and the content of IL-12, TNF-α and INF-γ which are factors (cytokines) related to an immune function. The mixed fermentation product is obtained by fermenting pears and gingers which have been used as food for a long time. Therefore, the mixed fermentation product is highly safe, does not cause side effects, and thus can be widely used for a pharmaceutical composition or a vegetable composition related to immunity enhancement.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a composition for enhancing immunity comprising a fermented product of ginger,

The present invention relates to a composition for enhancing immunity, which comprises a fermented product of a pear and a ginger as an active ingredient.

Immunity is largely divided into the innate immunity that has been born and the acquired immunity which is obtained by adaptation to life and the like. The congenital immunity is also called natural immunity, responds to the antigen nonspecifically, and does not show any special memory effect.

Examples of the congenital immune system include skin, mucous tissue, gastric acid, which inhibits the entry of an antigen, macrophages responsible for phagocytosis to remove intruders entering the body, and phagocytes such as polymorphonuclear leukocytes. This congenital immune defenses most infections in practice. On the other hand, acquired immunity has a memory effect on the first infected antigen, and when the infiltration occurs again, it specifically reacts and effectively removes the antigen.

When this immune function deteriorates, various immune diseases such as asthma, seasonal or perennial rhinitis, allergic sinusitis, conjunctivitis, atopic dermatitis, urticaria, red blood cell hemolysis, acute glomerulonephritis and the like may occur. Such immune diseases are more important to prevent than treatment, and currently commercial drugs are difficult to take for prevention. There has been a need for a method for preventing and treating immune dysfunction without the use of immunostimulants which have the potential for side effects.

In response to these demands, many studies have been conducted on new drug researches and redevelopments that can prevent and treat immune dysfunction. Particularly, interest has been focused on the development of immunopotentiators or anticancer substances in natural products with few side effects .

The concept of an immune enhancer enhancing the immune function has been suggested by Roman and it has been shown that administration of any immunopotentiating substance together with the vaccine further enhances the effect of the vaccine as compared with administration of the vaccine alone Respectively. The term immunostimulant refers to any substance which raises the specific or nonspecific, cellular and humoral immune response of the host. Recently, immune enrichment has been used in the treatment of infections and tumors, which has attracted much attention.

10-0687468B 10-20050050072A

It is an object of the present invention to provide a composition for treating, ameliorating or preventing obesity, which comprises a fermented product of ginseng and ginger as an active ingredient.

In order to accomplish the above object, the present invention provides a composition for enhancing immunity. The immunoconjugate composition may be a pharmaceutical composition or a pharmaceutical composition.

Wherein the composition for immunomodulation is prepared by pulverizing a mixed fermented product of a pear and a ginger, specifically a pear and a ginger, respectively, to prepare a pulverized product and a ginger pulverized product; Preparing a fermentation broth by adding sugar to the pulverized product, fermenting the fermented product for 34 months, and then centrifuging to separate the fermentation broth; Preparing ginger fermentation broth by adding sugar to the ginger powder, fermenting the fermented product for 22 months, and then separating the fermentation broth by centrifugation; And mixing the fermentation broth and ginger fermentation broth at a content of 65 wt%: 35 wt% (g: ginger), based on the total weight of the mixture, to prepare a fermented product of ginseng and ginger, And the ginger fermented product of the present invention can be used as an active ingredient.

The present invention also provides a composition for enhancing immunity to achieve the above object. The immunoconjugate composition may be a food composition. The food composition may be a functional food composition.

The inventors of the present invention have continued research on the development of materials from natural sources in order to find a novel composition for immune enhancement that can be used for a long period of time without any side effects in vivo and can prevent and treat immune dysfunction. As a result, The contents of IL-12, TNF-α and INF-γ, which are the cytokines related to the weight of kidney and thymus, changes in leukocyte count and immune function, were measured by the mixture of fermented broth and ginger fermented product. And thus can be effectively used as an immunostimulating agent. Thus, the present invention has been completed.

Unless otherwise specified in the present specification, an effective component means a component that provides an immune function improving effect in a composition, specifically, a pharmaceutical composition or a food composition.

Unless specifically stated herein, an immunological disease is a concept that includes all kinds of diseases caused by an immune system abnormality in a living body. Specifically, the term " immunological disease " includes not only an inflammatory disease, an allergic disease, cancer, cold, asthma, , Allergic conjunctivitis, atopic dermatitis, allergies, rheumatoid arthritis, Alzheimer's disease, autoimmune thyroid disease, inflammatory bowel disease, aplastic anemia, lupus erythematosus and psoriasis.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a composition for enhancing immunity, which comprises a fermented product of a pear and a ginger as an active ingredient. The immunoconjugate composition may be a pharmaceutical composition or a pharmaceutical composition. Specifically, the immunoconjugate composition may be a pharmaceutical composition for preventing or treating immune diseases.

The pear is a fruit of a pear, and the portion of the fruit that can be eaten is about 80%, the content of water is 85% to 88%, the main ingredient is a carbohydrate, and the sugar such as fructose and sucrose, , And organic acids such as citric acid, as well as components such as vitamin B and vitamin C, and is rich in fiber and minerals. It is mainly effective against bronchial diseases, and helps defecation and diuretic action.

The pear ( Pyrus) serotina ) is a vernacular word in the rose and pear tree. It is about 2 m to 3 m in height. The leaves are ovate and serrate, and three white flowers bloom in spring. The pear tree is also referred to as a tree or a tree. There are several kinds of wild varieties such as perennials, southern pear, and luminous trees. Recently, cultivated varieties include.

The ginger ( Zingiber officinale ) is a perennial plant belonging to the ginger tree ginger and ginger in the monocotyledonous plant. Southeast Asia is the origin and is distributed as vegetable. The rootstock grows sideways, is fleshy, lumpy, yellowish, and has a pungent flavor and a fragrant smell. At each node of the rootstock, a fake stem made of a sheath is straight, with a height of 30 cm to 50 cm, and two leaves on the top. Leaves are slanted, bar-shaped, with narrow ends on both ends and long bottom leaves. The rootstock is dried and used as a spice for bread, exoskeleton, curry, sauce, peeled, boiled, put in syrup, and made into ginger tea and ginger. In one room, dried roots are used as medicines for health, which stimulates appetite, has effects on digestion, vomiting and diarrhea, promotes blood circulation, and has anti-inflammatory and analgesic effects.

The mixed fermented product of the pear and ginger may be a mixture of the fermentation broth and the ginger fermentation broth. Preferably, the mixed fermented product of the pear and ginger is pulverized into a pear and a ginger to prepare a pulverized product and a ginger pulverized product step; Preparing a fermentation broth by adding sugar to the pulverized product, fermenting the fermented product for 34 months, and then centrifuging to separate the fermentation broth; Preparing ginger fermentation broth by adding sugar to the ginger powder, fermenting the fermented product for 22 months, and then separating the fermentation broth by centrifugation; And mixing the fermentation broth and ginger fermentation broth at a content of 65 wt%: 35 wt% (g: ginger), based on the total weight of the mixture, to prepare a fermented product of ginseng and ginger, And a method for producing a fermented product of a ginger and a pear containing ginger.

The immunoconjugate composition of the present invention may be a pharmaceutical composition for preventing or treating immune diseases.

The immunological disease refers to a disease caused by an immune system abnormality in the body, and specifically refers to any one selected from the group consisting of an inflammatory disease, an allergic disease and cancer, more specifically, a cold, asthma, pneumonia, allergic rhinitis, Inflammatory bowel disease, anorexia, conjunctivitis, atopic dermatitis, allergy, rheumatoid arthritis, autoimmune thyroid disease, inflammatory bowel disease, aplastic anemia, lupus erythematosus, psoriasis and cancer.

The mixed fermented product of the ginseng and the ginseng was tested by using an experimental animal. As a result, it was found that the concentration of IL-12, TNF-α and INF-γ, which are cytokines related to the renal and thymus weight, As a result of checking the contents, it was confirmed that the ability to improve the immunological activity was superior to that of the case of simply treating the pear extract or ginger extract.

The composition for enhancing immunity comprising the mixed fermented product of the ginger and the ginger may further contain, in addition to the active ingredient, a nutritional supplement, a vitamin, an electrolyte, a flavoring agent, a colorant, a thickening agent, a pectic acid and its salt, Organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like.

The carrier, excipient or diluent may be selected from lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acicia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline In the group consisting of celluloses, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol, liquid paraffin, But the present invention is not limited thereto. Any conventional carrier, excipient or diluent may be used. The components may be added to the active ingredient, that is, to the mixed fermentation product of the pear and ginger, independently or in combination.

The composition for enhancing immunity comprising the mixed fermented product of the above ginger and pear ginger as an active ingredient preferably contains 0.001% by weight to 99.9% by weight, preferably 0.1% by weight to 99% by weight, of the above-mentioned effective ingredient relative to the total weight of the composition, May comprise from 1% to 50% by weight. The content of the additional component may be added in the range of 0.1% by weight to 20% by weight based on the total weight of the immunoconjugating composition, but is not limited thereto.

In addition, when the composition for enhancing immunity comprising the mixed fermented product of gum and gingiva as an active ingredient is weakened, it is possible to use conventional fillers, extenders, binders, disintegrators, surfactants, anti-coagulants, lubricants, wetting agents, Or preservatives, and may be used either orally or parenterally.

Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like. These solid preparations can be prepared by mixing at least one excipient, for example, Starch, calcium carbonate, sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweetening agents, have.

In addition, the formulation of the composition for enhancing immunity comprising the mixed fermented product of ginseng and gingiva of the present invention as an active ingredient may be in a preferable form depending on the method of use, and in particular, Release by the methods known in the art. Examples of the specific formulations include granules, powders, syrups, liquids, suspensions, tablets, injections, main tablets, cataplasma, capsules, soft or hard gelatin capsules and the like.

Further, the composition for enhancing immunity comprising the mixed fermented product of ginseng and ginger of the present invention as an active ingredient can be produced by using a suitable known method in the art or by using the method of Remington ' s Pharmaceutical Science (recent edition), Mack Publishing Company , Easton PA). ≪ / RTI >

The dose of the composition for enhancing immunity comprising the mixed fermented product of ginseng and ginger according to the present invention as an active ingredient can be suitably adjusted by a person skilled in the art in consideration of the administration method, the age, sex and weight of the recipient, Can be selected. For example, the composition for enhancing immunity comprising the mixed fermented product of ginseng and gingiva of the present invention as an active ingredient may be used in an amount of 0.0001 mg / kg to 1000 mg / kg, / kg to 500 mg / kg. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

In addition, the composition for enhancing immunity comprising the mixed fermented product of ginseng and gingiva according to the present invention may further comprise a compound having a known immune enhancing effect or an extract for a natural substance in addition to the effective ingredient, And 5 parts by weight to 200 parts by weight with respect to 100 parts by weight of the component.

The present invention also relates to a food composition for enhancing immunity, which comprises a fermented product of a pear and ginger as an active ingredient. The food composition may be a health functional food composition.

The mixed fermented product of the pear and ginger may be a mixture of the fermentation broth and the ginger fermentation broth. Preferably, the mixed fermented product of the pear and ginger is pulverized into a pear and a ginger to prepare a pulverized product and a ginger pulverized product step; Preparing a fermentation broth by adding sugar to the pulverized product, fermenting the fermented product for 34 months, and then centrifuging to separate the fermentation broth; Preparing ginger fermentation broth by adding sugar to the ginger powder, fermenting the fermented product for 22 months, and then separating the fermentation broth by centrifugation; And mixing the fermentation broth and ginger fermentation broth at a content of 65 wt%: 35 wt% (g: ginger), based on the total weight of the mixture, to prepare a fermented product of ginseng and ginger, And a method for producing a fermented product of a ginger and a pear containing ginger.

The term " food " means a natural product or a processed product containing one or more nutrients, preferably a state of being able to be eaten directly through a certain degree of processing, Beverages, food additives, beverage additives, and the like.

Such foods include, for example, various foods, beverages, gums, tea, vitamin complexes, and health functional foods. In addition, in the present invention, the food may contain special nutritional foods (e.g., crude oil, spirits, infant food, etc.), meat products, fish products, tofu, jelly, noodles (Such as soy sauce, soybean paste, hot pepper paste, mixed sauce), sauces, confectionery (eg snacks), dairy products (eg fermented milk, cheese), other processed foods, kimchi, pickled foods But are not limited to, fruits, vegetables, beverages, fermented beverages, etc.), natural seasonings (e.g., ramen soup, etc.). The food, the health functional food, the beverage, the food additive and the beverage additive can be produced by a usual production method.

In the present invention, the health functional food refers to a food group imparted with added value to function or express the function of the food by physical, biochemical, biotechnological techniques, etc., or to control the biological defense rhythm of the food composition, Means a food that is designed and manufactured so that the weight control function related to recovery and the like is sufficiently expressed in living bodies.

The health functional food may include food-acceptable food supplementary additives, and may further include suitable carriers, excipients and diluents conventionally used in the production of health functional foods.

The drink refers to a generic term for eliminating thirst or drinking to enjoy a taste, and is intended to include a health functional drink. The beverage is not particularly limited as long as it contains, as an essential ingredient, a mixed fermented product of the pear and ginger as an active ingredient as the essential ingredient at the indicated ratio, and it is also possible to add various flavors or natural carbohydrates, .

Examples of such natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose such as maltose, sucrose and the like and polysaccharides such as dextrins, cyclodextrins and the like, and Xylitol, sorbitol, and erythritol. Natural flavors (tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate may be generally about 1 to 20 g, preferably 5 to 12 g per 100 ml of the food composition of the present invention. In addition, the composition of the present invention can be used for the production of natural fruit juice, fruit juice drink, Can also be added.

In addition to the above, the food composition of the present invention can be used as a flavoring agent such as a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, Salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. These components can be used independently or in combination. The ratio of these additives is not so important, but may be selected in the range of 0 to 2,000 parts by weight per 100 parts by weight of the mixed fermented product of the present invention.

The health-functional beverage is used to control the bio-defense rhythm of the beverage group or beverage composition to which the added value is imparted so that the function of the beverage functions for a specific purpose by physical, biochemical, biotechnological, or the like, Means a beverage which has been designed so as to sufficiently express the body controlling function related to the living body.

The health functional beverage is not particularly limited as long as it contains the fermented product of the present invention and the mixed fermented product of ginger as an essential ingredient at the indicated ratio, and may be added with various flavors or natural carbohydrates, ≪ / RTI >

Examples of such natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose such as maltose, sucrose and the like and polysaccharides such as dextrins, cyclodextrins and the like, and xylitol , Sorbitol, and erythritol. Natural flavors (tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably 5 to 12 g per 100 ml of the composition of the present invention.

In addition, in the food composition for preventing or improving obesity, which comprises a fermented product of a mixture of pear and ginger as an active ingredient, the effective ingredient may be contained in an amount of 0.01 to 15% by weight of the total food, 0.0 > g < / RTI > to 5 g, preferably 0.3 g to 1 g.

The mixed fermented product of ginseng and ginseng, which is an effective ingredient of the present invention, is more effective than a simple fermented product of ginseng or ginger, in comparison with the cytokines IL-12, TNF- α and IFN-γ, respectively, were found to be excellent in immunostimulating ability. In addition, since the pear and ginger have been used for a long period of time, they are excellent in safety and do not cause side effects, so they can be widely used as a pharmaceutical composition or a plant composition related to immunity enhancement, Is recognized as having excellent industrial effect.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Example . Mix of pear and ginger Fermented  Produce

The mixed fermented product of pear and ginger was prepared as follows.

First, the pear was purchased on Nov. 4, 2011, and the ginger was purchased on Nov. 8, 2011 in Jeonju, Jeonju region. Each of the purchased vessels and ideas was separately cleaned in running water and finely pulverized using a pulverizer (Nisshin ryoki model-75, Japan). 26.5 kg of each pulverized pulverized product and 26.5 kg of ginger powder were put into a fermenter jar and 23.5 kg of brown sugar (Samyang Co., Ltd., Korea) was poured in. Then, the temperature condition of 5.8 ° C to 29.4 ° C and absolute humidity Fermentation was carried out for 34 months in the case of boats and 22 months in the case of ginger while maintaining the humidity condition of 35% to 95%.

During the fermentation period, once a month from the start of fermentation until two months after the start of fermentation, three times a week, from three months after the start of fermentation to six months, twice a month, and six months from the start of fermentation, Was artificially stirred with the whole fermentation product.

After each fermentation period elapsed, the fermentation broth was collected using a centrifugal extractor (dehydrated 3 horsepower centrifugal extractor, Yuseong, Korea) and stored in a storage tank (2 ton stainless steel vessel, Hansung Ef & . After 2 months, the mixed fermented broth and ginger were prepared by mixing 6.5 kg of ginger fermentation broth and 3.5 kg of ginger, respectively.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (5)

And a mixed fermented product of ginseng and ginger as an active ingredient. The method according to claim 1,
Wherein the mixed fermented product of the pear and ginger is prepared by pulverizing a pear and a ginger to produce a pear pulverized product and a ginger pulverized product; Preparing a fermentation broth by adding sugar to the pulverized product, fermenting the fermented product for 34 months, and then centrifuging to separate the fermentation broth; Preparing ginger fermentation broth by adding sugar to the ginger powder, fermenting the fermented product for 22 months, and then separating the fermentation broth by centrifugation; And mixing the fermentation broth and ginger fermentation broth at a content of 65 wt%: 35 wt% (g: ginger), based on the total weight of the mixture, to prepare a fermented product of ginseng and ginger, And a fermented product of a mixture of ginger and a ginger comprising the ginger. Which comprises a fermented product of pear and ginger as an active ingredient. The food composition according to claim 3, wherein the food composition for immunomodulation is a health functional food composition. The method of claim 3,
Wherein the mixed fermented product of the pear and ginger is prepared by pulverizing a pear and a ginger to produce a pear pulverized product and a ginger pulverized product; Preparing a fermentation broth by adding sugar to the pulverized product, fermenting the fermented product for 34 months, and then centrifuging to separate the fermentation broth; Preparing ginger fermentation broth by adding sugar to the ginger powder, fermenting the fermented product for 22 months, and then separating the fermentation broth by centrifugation; And mixing the fermentation broth and ginger fermentation broth at a content of 65 wt%: 35 wt% (g: ginger), based on the total weight of the mixture, to prepare a fermented product of ginseng and ginger, And a fermented product of a mixture of ginger and a ginger comprising the fermented product.