KR20150085330A - A method for preparing artemisia extract with a reduced content of hazardous substance for treatment of gastrointestinal disorders - Google Patents

Abstract

The present invention relates to a manufacturing method of an Artemisia herb extract with reduced harmful substance content for treating gastrointestinal disorders using active carbon, wherein an Artemisia herb extract is extracted from Artemisia herb leaves with ethanol or propanol. According to the present invention, a manufacturing method of an Artemisia herb extract using active carbon can manufacture an Artemisia herb extract with reduction of benzopyrene derived from crude drugs, with reinforced stability, and without changing medicinal effects. Accordingly, an Artemisia herb extract manufactured by a manufacturing method of the present invention is capable of maintaining medicinal effects and effectively reducing benzopyrene which is a harmful substance.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for preparing a lyophilized extract for treating gastrointestinal diseases,

The present invention relates to a method for manufacturing a leaf extract, which can maintain the efficacy of treating gastrointestinal diseases while reducing the content of benzopyran, which is a harmful substance, in leaf extracts.

Benzo [a] pyrene is a yellow crystalline solid belonging to the polycyclic aromatic hydrocarbons (PAHs) group and is produced by incomplete combustion at temperatures between 300 and 600 ° C.

It is present mainly in coal tar, car exhaust gas (especially diesel engine), tobacco smoke, and also in foods that have not been cooked / processed such as agricultural products, fish and shellfish due to environmental pollution and carbohydrates and proteins , Lipids, etc. are also decomposed.

Benzopyran is an endocrine disrupter and carcinogenic substance and is included in the priority list for risk assessment of CODEX and JECFA (FAOHHO Joint Food Additives Expert Committee) And the like. The International Agency for Research on Cancer (IARC) recently upgraded benzopyran to a human carcinogen in Group 1. There is a problem with benzopyrene in food, too. In recent years, interest in reduction of benzopyran in edible oil such as olive oil has become a social problem.

The regulation of benzoprene produced in the edible oil manufacturing process is managed by 2.0 ppb of EU, 5.0 ppb of Spain, and 10 ppb of China, and the recommended specification is set to 2.0 ppb or less in accordance with the most strictly regulated EU standards in Korea have.

In order to reduce such harmful benzopyran, various biological, physical / chemical methods have been disclosed. In particular, a method for reducing benzopyrene produced in edible oils in foods has been proposed, for example, in a purification exhaust system and a reduction method by adjusting the roasting temperature Korean Patent Laid-Open Publication No. 2009-38548.

Conventional methods for reducing benzopyrene in edible oils include methods of absorbing edible oil in clay, carbonizing the edible oil, activating the carbon, and using acid-activated clay as an absorbent, are disclosed in U.S. Patent No. 5,218,132 Lt; / RTI > The carbonization and activation step of the edible oil in the US patent is performed by heating in the presence of zinc chloride, which is an active agent, and the activation temperature should be 250 ° C or higher. However, the above method can be applied to general edible oil, but in case of pressed oil, especially red pepper seed oil, sesame oil and perilla oil, the flavor and color may change due to clay or temperature.

A method for producing a pressed oil in which benzoprene is reduced by using activated carbon for edible oil is known (Korean Patent Application No. 2007-103889), but the prior art is described at a high temperature of 60 to 110 ° C .

However, when the activated carbon reduction process used for such conventional foods is used, a pretreatment process is required at a high temperature. Therefore, the herbal medicine extract is not suitable for use because it has a problem of changing the active ingredient.

In general, most of herbal medicines are known to contain a certain amount of benzopyran, which is a harmful substance due to modern environmental pollution (monitoring of benzopyran content in Chinese medicines, Digital Policy Research Vol.10, No.7 (2012.8)) . In order to manage the benzopyran that occurs during the pretreatment process of herbal medicine, the Korea Food and Drug Administration (KFDA) sets standards for benzopyran (5ppb), which is a substance against herbal medicine, -13).

Leaf extract promotes the production of HSP, VEGF, and EGF by promoting the production of prostaglandin, promotes mucus secretion, promotes gastric mucosal blood flow, protects gastric mucosa, promotes healing of damaged gastric cells, , Korean Patent Publication No. 10-1000951, Korean Laid-Open Patent Publication No. 2009-0088343 and the like are known to be extracted and manufactured using a solvent belonging to ICH Guideline Class 3 such as alcohol and isopropyl alcohol.

These lyophilized extracts are enriched with lyocell, which is a raw material herb medicine, and the affinity of benzopyran, which is a harmful substance in the lyophilized lyophilus, to organic solvents such as ethanol and isopropyl alcohol used for extracting effective components such as epaetin, Which may be contained in some contaminated herbs Benzopyran can be included as a leaf extract.

The benzopyran, which may be present in trace amounts in the leaf extract, has a very small amount of daily intake, but the amount of exposure to the human body is much smaller than that of other herbal medicines and foods, but the harmful substances are also scientifically causally revealed in small amounts There is a possibility of showing unacceptable risks. Therefore, removal of benzopyrene from the leaf extract is an important task that must be accomplished by those skilled in the art.

Conventionally, it has been known to use activated carbon in herbal medicines in Korean Patent Registration No. 10-0577396 and Korean Patent Laid-Open Publication No. 2009-0080459, but it has been known to use activated carbon for the purpose of removing chlorine, chloroform, And there is no particular description about selectively reducing harmful substances such as benzopyran and minimizing changes in the content of active ingredients.

Therefore, there is no known method for reducing benzopyran in the extract of the present invention. In the case of using the method used in food (Korean Patent Laid-Open Publication No. 2009-38548), the active ingredient in the leaf extract is denatured by heat to decrease the content And there is a problem that it may affect the drug efficacy.

Active carbon is a special carbon that is calcined at high temperature using mainly coconut shell, wood, and coal as a raw material. It is a collection of amorphous carbon that has fine pores of molecular size well developed during the activation process. The raw materials for the production of activated carbon can be divided into food materials, animal matter, minerals, and industrial wastes. In the case of minerals, coal and petroleum can be distinguished. In general, powdered activated carbon is produced using a raw material of a food material, and charcoal, coconut shell, coal and the like are used for producing granular activated carbon.

The theory of adsorption of activated carbon is a phenomenon in which specific components in gas or liquid are concentrated at the solid-liquid, gas-liquid, and liquid-liquid interfaces. Adsorption is usually classified into physical adsorption and chemical adsorption depending on the type of adsorption. The force that governs the physical adsorption is the van der Waals force, and the chemical adsorptive forces such as ionic or covalent bonds dominate the chemical adsorption. In the case of physical adsorption, the solute sticks to the surface of the adsorbent when the attractive force between the solute and the adsorbent is greater than the attractive force between the solute and the solvent.

The inventors of the present invention found that, while proceeding to lower the content of benzopyrin derived from raw materials and the like in the production method of the leaf extract, surprisingly, the pharmacological activity of benzopyran, which is a harmful substance, -13), which is a suitable level, to 30% level, thereby completing the present invention.

The present invention overcomes the disadvantages of the conventional production method in which the benzopyrene derived from the herbal medicine is converted into the extract in the production of the bean curd extract and only the content of the harmful substance benzopyrene is selectively The present invention also provides a method for producing a lysolecule extract, which is effective to treat gastrointestinal diseases in an amount equivalent to that of the extract when the activated carbon is not treated.

The present invention relates to a method for producing a leaf extract for treating gastrointestinal diseases, wherein a leaf extract obtained by extracting leaf leaves with ethanol or propanol is reduced in the content of harmful substances using activated carbon.

In order to attain the above object, the lady extract according to the present invention should have the following characteristics.

First, the effect of treatment of gastrointestinal disease should be maintained to the same extent,

Second, the content of benzopyran, which is a harmful substance derived from a herbal medicine, should be sufficiently reduced.

In the present invention, activated carbon is used to reduce the content of benzopyrene derived from leaf leaves, and the leaf extract treated with 0.1 to 20% (w / w) activated carbon with respect to the weight of leaves of leaf leaves has a significantly reduced benzopyran content, The content of postural oocyte showed no significant change, indicating the effect of treating the gastrointestinal disease of the extract of the oocyte to an extent comparable to that of the untreated group.

In the present invention, when 0.1% (w / w) activated carbon is used relative to the leaf weight of leaves, about 80% (w / w) of the toxic substance benzopyrene is reduced, and the content of benzopyran remaining in the leaf extract It is found that the effect of the gastric disease is equivalent to that of the activated carbon because there is little change in the contents of the ophatholin and postosocidin compared to the untreated group.

In the present invention, when 20% (w / w) activated carbon is used relative to the leaf weight of leaves, about 99% (w / w) of the toxic substance benzopyrene is reduced, Compared with the untreated group, the content of yupatiline and posacidin was slightly decreased, but the effect of treatment of gastrointestinal diseases was found to be equivalent.

However, the leaf extracts using 30-100% (w / w) activated carbon based on the weight of the leaflets can remove all of the benzopyran, which is a harmful substance, but the active ingredients, .

Accordingly, the present invention is characterized by using 0.1-20% (w / w) activated carbon as the weight of leaf blade leaves, preferably 0.1-5% (w / w) activated carbon.

In the present invention, leaf leaves can be extracted with ethanol or propanol. When the activated carbon filtration process is applied, there is no difference in the ability to reduce benzopyran content in the concentration range of 70 to 100% (v / v) Ethanol is preferred. Propanol also uses 1-propanol or 2-propanol with a 100% (v / v) concentration.

In addition, the present invention provides a method for reducing the benzopyran content by using 0.1 ~ 20% (w / w) activated carbon based on the leaf blade weight of the leaf extract obtained by extracting leaf leaves with ethanol or propanol.

In the present invention, the step of reducing benzopyran in the leaf extract may be a treatment during extraction of leaflets, a treatment before concentration after extracting leaves of leaflets, or a treatment after concentrating and re-dissolving leaf extracts.

In the present invention, the process of treating leaflets during leaf extraction is to remove benzopyrene by extracting leaves of leaflets with a solvent and circulatingly filtering the leaves using a column packed with activated charcoal, And the filtrate is filtered to circulate and remove the benzopyrene. The lyophilized extract is concentrated and the lyophilized product is recovered by extracting the leaves of the lyophilized leaves, filtering and concentrating the filtrate under reduced pressure. It is a method of removing benzopyrene by circulation filtration using a column packed with activated carbon after being re-dissolved as a solvent.

Both the benzopyran reduction rate and the reduction amounts of the active ingredients of the leaves, i.e., the amounts of essential oil and pfu tiline, can be obtained in all the solvents.

The present invention provides a method for preparing a leaf extract, which overcomes the disadvantages of the conventional production method in which the benzopyrene derived from the herb medicine is converted into an extract in the production of the leaf extract, reduces the benzopyrene content and maintains the active substance content, .

FIG. 1 is a graph showing the contents of benzopyran, yaffatilin and paclitaxel as compared to the untreated activated carbon of the leaf extract.
FIG. 2 is a graph showing an evaluation of gastric lesion inhibition of a leaf extract according to the addition of an activated charcoal filter process to an activated carbon non-treated leaf extract. FIG.

Hereinafter, the present invention will be described in detail with reference to examples.

However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

≪ Example 1 > Preparation of ethanol extract of 95% (v / v) treated with 20% (w / w) of activated carbon compared to herbal medicine

100 g of leaf lobules were air-washed with 800 ml of 95% (v / v) ethanol for 20 hours at room temperature, circulated and filtered using a column packed with 20 g of activated charcoal, and then about 95% (v / v) And the mixture is cooled for 4 hours, and the column charged with 20 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

≪ Example 2 > Production of 95% (v / v) ethanol extract treated with 10% (w / w) of activated carbon compared to herbal medicine

100 g of leaf lobules were air-washed with 800 ml of 95% (v / v) ethanol for 20 hours at room temperature, circulated and filtered using a column packed with 10 g of activated charcoal, and then about 95% (v / v) And the mixture is cooled for 4 hours and the column charged with 10 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

≪ Example 3 > Production of 95% (v / v) ethanol extract treated with 5% (w / w) of activated carbon compared to raw herbal medicine

100 g of leaf lobules were air-washed with 800 ml of 95% (v / v) ethanol for 20 hours at room temperature, circulated and filtered using a column packed with 5 g of activated charcoal, and then about 95% (v / v) And the mixture is cooled for 4 hours and the column filled with 5 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

<Example 4> Production of 95% (v / v) ethanol extract treated with activated carbon 1% (w / w)

100 g of each leaf of leaves was air-cooled with 800 ml of 95% (v / v) ethanol for 20 hours at room temperature, and circulated and filtered using a column packed with 1 g of activated charcoal. Then, about 800 ml of 95% And the mixture is cooled for 4 hours, and a column packed with 1 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Example 5 > Production of 95% (v / v) ethanol extract treated with 0.1% (w / w) of activated carbon compared to raw herbal medicine

100 g of leaf lobules were air-washed with 800 ml of 95% (v / v) ethanol for 20 hours at room temperature, and then subjected to circulation filtration using a column packed with 0.1 g of activated carbon. Then, 95% (v / v) ethanol 800 ml is added and the mixture is cooled for 4 hours and the column filled with 0.1 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Example 6 > Preparation of 95% (v / v) ethanol extract treated with activated carbon 5% (w / w)

100 g of leaf lobules were extracted by cooling with 800 ml of 95% (v / v) ethanol for 20 hours at room temperature, filtered, and then about 800 ml of 95% (v / v) ethanol was added to the residue. . The total filtrate was combined and the column charged with 5 g of activated carbon was circulated and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Example 7 > Preparation of ethanol extract of 95% (v / v) treated with activated carbon 5% (w / w)

100 g of the leaf lobes were washed with 800 ml of 95% (w / w) ethanol for 20 hours at room temperature, filtered, and then about 800 ml of 95% (w / w) ethanol was added to the residue. . The total filtrate was combined and concentrated under reduced pressure to obtain about 5 g of an extract. The extract was redissolved in 1 L of 95% (v / v) ethanol, and then subjected to circulation filtration using a column packed with 5 g of activated carbon, and further concentrated under reduced pressure to obtain a soft extract of about 5 g.

The following examples were prepared by extracting leaf leaves with 70% (v / v), 80% (v / v), 85 (v / v), 90% (v / v) 5% (w / w) of activated charcoal as compared with the raw herbal medicine was used as in Example 3 to prepare a lyophilized extract according to the present invention.

&Lt; Example 8 > Preparation of 70% (v / v) ethanol extract-activated carbon treatment-

100 g of leaf lobules were air-washed with 800 ml of 70% (v / v) ethanol for 20 hours at room temperature, and then circulated by using a column packed with 5 g of activated charcoal. Then, about 800 ml of 70% (v / v) And the mixture is cooled for 4 hours and the column filled with 5 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Example 9 > Preparation of 80% (v / v) ethanol extract-activated carbon treatment-

100 g of leaf lobules were air-washed with 800 ml of 80% (v / v) ethanol for 20 hours at room temperature, and then subjected to circulation filtration using a column packed with 5 g of activated charcoal. Then, about 80 ml of v / v ethanol And the mixture is cooled for 4 hours and the column filled with 5 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

Example 10 Preparation of 85% (v / v) Ethanol Extract-Activated Carbon Treatment-

100 g of leaf lobules were air-cooled with 800 ml of 85% (v / v) ethanol for 20 hours at room temperature, and then subjected to circulation filtration using a column packed with 5 g of activated carbon. Then, about 800 ml of 85% (v / v) And the mixture is cooled for 4 hours and the column filled with 5 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Example 11 > Preparation of 90% (v / v) Ethanol Extract-Activated Carbon Treatment-

100 g of leaf lobules were air-washed with 800 ml of 90% (v / v) ethanol for 20 hours at room temperature, and then circulated by using a column packed with 5 g of activated carbon. Then, about 800 ml of v / v ethanol And the mixture is cooled for 4 hours and the column filled with 5 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Example 12 > Production of 100% (v / v) ethanol extract-activated carbon treatment-

100 g of the leaf lobes were circulated with 800 ml of 100% (v / v) ethanol for 20 hours at room temperature and then circulated through a column packed with 5 g of activated carbon. Then, about 800 ml (v / v) And the mixture is cooled for 4 hours and the column filled with 5 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

In the following example, leaf leaves were extracted with 100% (v / v) 1-propanol and 100% (v / v) 2-propanol in place of ethanol as an extraction solvent, / w) was used to produce a lentil extract according to the present invention.

&Lt; Example 13 > Preparation of 100% (v / v) 1-propanol extract-activated carbon treatment-

100 g of the leaf livers of 100 ml (v / v) 1-propanol were air-cooled for 20 hours at room temperature, circulated and filtered using a column packed with 5 g of activated charcoal, -Propanol is added, and the mixture is cooled for 4 hours and the column filled with 5 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Example 14 > Preparation of 100% (v / v) 2-propanol extract-activated carbon treatment-

100 g of the leaf lobes of each well were air-washed with 800 ml of 100% (v / v) 2-propanol for 20 hours at room temperature, and then subjected to circulation filtration using a column packed with 5 g of activated charcoal. -Propanol is added, and the mixture is cooled for 4 hours and the column filled with 5 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Comparative Example 1 & Production of 95% (v / v) ethanol extract treated with 100% (w / w) of activated carbon compared to herbal medicine

100 g of leaf lobules were air-washed with 800 ml of 95% (v / v) ethanol for 20 hours at room temperature, circulated and filtered using a column packed with 100 g of activated carbon, and then about 95% (v / v) And the mixture is cooled for 4 hours. Then, the mixture is subjected to circulation filtration using a column packed with 100 g of activated carbon. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Comparative Example 2 & Production of 95% (v / v) ethanol extract treated with 50% (w / w) of activated carbon compared to herbal medicine

100 g of leaf lobules were air-washed with 800 ml of 95% (v / v) ethanol for 20 hours at room temperature, circulated and filtered using a column packed with 50 g of activated carbon, and then about 95% (v / v) And the mixture is cooled for 4 hours. A column filled with 50 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Comparative Example 3 & Production of ethanol extract of 95% (v / v) treated with 30% (w / w) of activated carbon compared to herbal medicine

100 g of leaf lobules were air-washed with 800 ml of 95% (v / v) ethanol for 20 hours at room temperature, and then circulated and filtered using a column packed with 30 g of activated charcoal. Then, about 800 ml of 95% (v / v) And the mixture is cooled for 4 hours, and the column charged with 30 g of activated carbon is circulated and filtered. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Comparative Example 4 & Preparation of 70% (v / v) ethanol extract-activated carbon treatment

100 g of leaf lobules are cooled with 800 ml of 70% (v / v) ethanol at room temperature for 20 hours, and then about 800 ml of 70% (v / v) ethanol is added to the residue. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Comparative Example 5 & Preparation of 80% (v / v) ethanol extract - activated carbon treatment -

100 g of leaf lobules are cooled with 800 ml of 80% (v / v) ethanol for 20 hours at room temperature, and then about 800 ml of 80% (v / v) ethanol is added to the residue. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Comparative Example 6 > Preparation of 85% (v / v) Ethanol Extract - Activated Carbon Untreated -

100 g of leaf lobules are cooled with 800 ml of 85% (v / v) ethanol at room temperature for 20 hours, and then about 800 ml of 85% (v / v) ethanol is added to the residue. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Comparative Example 7 & Manufacture of 90% (v / v) Ethanol Extract - Activated Carbon Untreated

100 g of leaf lobes are washed with 800 ml of 90% (v / v) ethanol for 20 hours at room temperature, filtered, and then about 800 ml of 90% (v / v) ethanol is added to the residue. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Comparative Example 8 > Production of 95% (v / v) Ethanol Extract-Activated Carbon Untreated-

100 g of the leaf lobules are washed with 800 ml of 95% (v / v) ethanol at room temperature for 20 hours, filtered, and then about 800 ml of 95% (v / v) ethanol is added to the residue. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Comparative Example 9 & Manufacture of 100% (v / v) Ethanol Extract - Activated Carbon Untreated -

100 g of the leaf livers are washed with 800 ml of 100% (v / v) ethanol for 20 hours at room temperature, filtered, and then about 800 ml of 100% (v / v) ethanol is added to the residue. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Comparative Example 10 & Production of 100% (v / v) 1-propanol extract-activated carbon treatment

100 g of the leaf livers of 100 ml (v / v) 1-propanol were cooled with 20 ml of 100% (v / v) 1-propanol for 20 hours and filtered. Filter. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

&Lt; Comparative Example 11 & Preparation of 100% (v / v) 2-propanol extract-activated carbon treatment

100 g of the leaf livers of 100% (v / v) 2-propanol were added to 800 ml of 100% (v / v) 2-propanol by cooling with ice water at room temperature for 20 hours, Filter. The total filtrate was combined and concentrated under reduced pressure to obtain a soft X-ray of about 5 g.

<Experimental Example 1> Effect of Removal of Hazardous Substances and Effects of Active Ingredients on Leaf Extract according to Amount of Activated Carbon Treatment

In order to examine the content of benzopyran and the content of active ingredient in the leaf extracts according to the amount of activated carbon, a 95% (v / v) ethanol lobe extract treated with activated carbon (Examples 1 to 5 and Comparative Examples 1 to 3) Respectively. A 95% (v / v) ethanol lobe extract (Comparative Example 8) not treated with activated carbon was used as a control. Each soft-tissue extract was evaluated using high performance liquid chromatography (HPLC) and the results are shown in Table 1 and FIG.

As a result of the test, as shown in Table 1, the leaf extract (Comparative Examples 1 to 3) using 30 to 100% (w / w) of activated carbon relative to the crude drug can remove all of the benzopyrene, Tiline, and posture osidine also showed large changes in content.

However, it can be seen that Examples 1 to 5 according to the present invention exhibit benzopyran reducing effect, and that the content of oil tiller and postural oocyte is not greatly changed. In particular, as can be seen from Example 5, even when the amount of activated carbon is 0.1% (w / w) as compared with the raw herbal medicine, it is found that benzopyran is reduced by about 80% (w / w).

<Experimental Example 2> Assessment of Gastric Lesion Suppression of Leaf Extract According to Amount of Activated Carbon Treatment

In Experimental Example 1, the 95% (v / v) ethanol leaf extract showed a change in the contents of benzopyran as a harmful component, and as an active ingredient, milk taurine and postural oocystin, depending on the amount of activated carbon. Herein, the gastric mucosal protection effect was evaluated in a normal hydrochloric acid-ethanol-induced injury rat model using the leaf extracts prepared in Examples 1 to 5, Comparative Examples 2, 3 and Comparative Example 8 (Comparative Example 1 Was excluded from the experiment because the amount of active ingredient was removed. The results are shown in Table 2 and Fig.

As can be seen from Table 2, Comparative Examples 2 and 3 can remove the amount of benzo [pi] lene, but the effect of the active ingredient,

However, in Examples 1 to 5 using 20% (w / w) or less of activated carbon, it was found that the effect of inhibiting the bleeding lesion was not different from the activated carbon untreated group.

Accordingly, the present invention provides a method for inhibiting the intestinal bleeding lesion of an equivalent amount of a leaf extract, while reducing benzopyran, which is a harmful substance compared to the untreated group, when using 0.1% (w / w) to 20% (w / . &Lt; / RTI &gt;

<Experimental Example 3> Evaluation of Removal of Hazardous Substances in Leaf Extracts by Activated Carbon Application Method

In the case of using 0.1% (w / w) to 20% (w / w) of the activated carbon as the weight of the leaf blade of the raw herbal medicine from the above Experimental Examples 1 and 2, the benzopyran, which is a harmful substance compared to the untreated group, It was found that the extracts of lobules showed the inhibitory effect on gastrointestinal lesions.

In this study, the content of benzopyran and the content of active ingredient in activated lees were investigated. (Example 3), extraction after filtration (Example 6), and extraction after re-filtration (Example 8) using 5% (w / w) of activated carbon. Each soft X-ray was evaluated using high performance liquid chromatography (HPLC), and the results are shown in Table 3.

Experimental Results As can be seen from Table 3, circulation filtration during extraction (Example 3), filtration after extraction (Example 6), extraction after filtration after re-filtration (use of 5% (w / Example 8) The method of applying benzopyran reduction rate and the decrease of the content of the active ingredient of ephedrine and posacin, respectively, in all solvents were similar.

Therefore, it can be seen that the activated carbon filtration process of the present invention is not different between the application points of the process.

<Experimental Example 4> Evaluation of Removal of Hazardous Substances in Solvent Leaf Extracts Using Activated Carbon Filter

Solvent comparative evaluation was carried out. Examples 3 and 8 to 14 were compared among the extracted examples using the activated carbon filtration process. Comparative Example 8 in which activated carbon was not treated was used as a control group. Each soft-drink extract was evaluated using high performance liquid chromatography (HPLC), and the results are shown in Table 4.

From Table 4, it can be seen from Table 4 that the benzopyran reduction rate and the reduction amount of the active ingredient of the ephedrine and posture oxidin, which are the active ingredients of the lysole, in the 70-100% (v / v) ethanol leaf extract and the 100% (v / v) isopropanol leaf extract, And it is expected that it will be able to exhibit the effect of inhibiting the intestinal bleeding lesion of the extract of the lobules equivalent to the activated carbon untreated group.

Claims (9)

A method for preparing a leaf extract for treating gastrointestinal diseases, which comprises extracting leaves of leaves with ethanol or isopropanol with 0.1 - 20% (w / w) activated carbon as a reducing agent to reduce harmful substances.
The method according to claim 1, wherein the harmful substance is benzopyran.
The method according to claim 1, wherein the ethanol is 70 ~ 100% (v / v) ethanol.
The method of claim 1, wherein the isopropanol is 100% (v / v) 1-propanol or 100% (v / v) 2-propanol.
5. The method according to any one of claims 1 to 4, wherein activated carbon is used in an amount of 0.1 to 5% (w / w) activated carbon based on the weight of leaflets.
A method of reducing benzopyran content by using 0.1 ~ 20% (w / w) activated carbon of a leaf extract extracted with ethanol or propanol as a leaf leaf.
The method for reducing benzopyran according to claim 6, wherein the step of reducing benzopyrene in the leaf extract is carried out during extraction of the leaf leaf.
The method for reducing benzopyran according to claim 6, wherein the step of reducing benzopyrene in the leaf extract is a treatment of concentrating leaf after extracting leaf leaves.
The method for reducing benzopyran according to claim 6, wherein the step of reducing benzopyran among the extracts of lyocell is performed by concentrating the lyocyte extract and then treating it by redissolution.

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