KR20150057271A - Sampling device with severing means and method of sampling or dispensing using the same - Google Patents

Abstract

The present invention relates to a sampling device and, more specifically, to a sampling device comprising: a sampling rod which is extended to have a predetermined length and has a sampling part at both ends in the longitudinal direction; and a sampling container to accept the sampling rod. The sampling rod includes one or more cutting grooves at the center in the longitudinal direction. The sampling container comprises: an accepting space to accept the sampling rod; and a cutting part to make the sampling rod which is inserted into the accepting space be cut by the cutting grooves and be accepted in the accepting space.

Description

TECHNICAL FIELD [0001] The present invention relates to a sampling device having a cutting means and a sampling method using the sampling device,

The present invention is in the field of sampling and / or dispensing devices.

In the case of various infectious diseases, the process of rapidly isolating and inspecting the infectious agent to identify a specific infectious source is very important for the shortening of the recovery time of the patient, the reduction of the treatment cost, and the prevention of pandemic.

For this purpose, sampling should be given priority from the patient. Usually, in the conventional method of collecting the sample, a sterilized cotton swab is used to acquire an infectious source present in the infected area, It is necessary to return the swabs from several times to several tens of times, to allow the infectious agent attached to the swab surface to be extracted into the solution for extraction, then to remove the solution from the swab as much as possible by pressing the swab . This process complicates the sampling process, and it is a very troublesome task to perform the test of the test person.

Therefore, there is a need to develop new apparatus and methods to overcome these problems that arise in the preprocessing or preparation of samples for sample collection and analysis.

Registered Utility Model No. 0250259 Public utility model 2011-0006452

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a device that can minimize contamination during sample processing, after sample collection, analysis of the sample, and improved convenience.

In order to achieve the above-mentioned object, in one aspect, the present invention provides a collecting rod extending to have a predetermined length and having a collecting part at one end in the longitudinal direction; And a sampling container in which the sampling rod is accommodated; Wherein the collecting rod includes at least one cutting groove formed in the center area in the longitudinal direction, and the collecting container includes a container body having a receiving space for accommodating the collecting rod therein, and the collecting rod inserted into the receiving space, And a cut-out portion which is cut through the cut-out portion and accommodated in the accommodating space.

According to an embodiment of the present invention, the sampling container according to the present invention may further include a cover provided on the top of the container body, and a through hole formed in the cover, the through hole being formed to penetrate the sampling bar, Wherein a part of the sampling rod is inserted into the receiving space through the through hole so that the part where the cutting groove is formed is bent while a part of the side of the sampling rod is in contact with the through hole, And the cutting and receiving of the rods is performed.

In another embodiment, the sampling container according to the present invention may further include a partition wall disposed in the accommodating space and dividing the accommodating space, and formed on all or a part of the longitudinal direction of the container main body, Wherein the collecting rod is cut and accommodated by bending the lower end of the collecting rod inserted through the through hole into contact with the partition wall or the inner peripheral surface of the container body.

In another embodiment, the sampling container further includes partition walls disposed in the accommodating space and dividing the accommodating space, and partition walls formed on all or a part of the longitudinal direction of the container main body, And a lower end of the collecting rod inserted through the through hole is connected to the lower end of the partition wall or the container body So that the sampling rod can be cut and accommodated by being bent in a state of being in contact with the inner peripheral surface.

In one embodiment of the present invention, the flow port is configured such that the partition wall has a gap with a predetermined gap from the bottom surface of the accommodation space so that the divided accommodation space flows through the lower part of the partition, And a penetrating window or slit is formed on the whole or part of the window.

In one embodiment according to the present invention, the partition is detachably configured from the container body or connected to the lower portion of the cover when the container body includes the cover, so that the partition is detachable together with the cover.

In one embodiment of the present invention, the inner peripheral surface of the container main body is formed with fixing grooves, in which both side ends of the partition wall are fitted and fixed.

In one embodiment of the present invention, the sampling container further includes a discharge port provided in the cover, wherein the discharge port and the through-hole are formed in the accommodating space divided by the partition when the sampling container includes the partition And are formed at corresponding positions.

In one embodiment of the present invention, the through-hole has a configuration in which an outer diameter becomes smaller toward the upward direction from the accommodation space, and the sampling rod is fitted to the through-hole so as to close the through- So that at least one portion in the longitudinal direction has a conical configuration.

In one embodiment according to the present application, the inner circumferential surface of the container body is further configured to include a position restricting portion at a position facing the partition wall.

In another aspect, the invention provides a method of sampling and / or dispensing using a sampling device according to the present disclosure.

In one embodiment, a method of collecting a sample using a sample collecting apparatus according to the present invention includes: a collecting rod extending to a predetermined length and having a collecting section at both ends in the longitudinal direction; And a sampling container in which the sampling rod is accommodated; Wherein the harvesting rod includes at least one cutting groove in a longitudinal center region thereof, the harvesting vessel including a container body having a receiving space for receiving the harvesting rod, and a harvesting rod inserted into the receiving space, And a cut-out portion that is cut through the groove and received in the accommodation space, the method comprising: contacting the harvesting portion formed on the harvesting rod with a biological sample; Inserting the sampling rod into the container body including the reagent; Cutting the sampling rod by breaking the cutting groove formed in the sampling rod by using the cut-out portion; And removing the cut harvesting rods.

According to another aspect of the present invention, there is provided a harvesting apparatus comprising: a harvesting rod extending to have a predetermined length and having a harvesting portion at one end in the longitudinal direction; And a collecting container in which the collecting rod is accommodated, wherein the collecting rod includes at least one cutting groove formed along the longitudinal direction, and the collecting container includes a container body having a receiving space for accommodating the collecting rod, And the cover includes a through hole formed to penetrate the collecting bar and a discharge port, and the discharge port and the through hole are connected to each other through a through hole, Wherein each of the sampling sections is formed at a position corresponding to a receiving space divided by the partition wall, the method comprising: contacting the sampling section formed on the sampling rod with a biological sample using the sampling device; Inserting the sampling rod into the container body including the reagent; Cutting the sampling rod by breaking the cutting groove formed in the sampling rod by using the cut-out portion; Removing the cut sampling rod; And discharging the mixture by inverting the container body.

According to another aspect of the present invention, there is provided a harvesting apparatus comprising: a harvesting rod extending to have a predetermined length and having a harvesting portion at one end in the longitudinal direction; And a collecting container in which the collecting rod is accommodated, wherein the collecting rod includes at least one cutting groove formed along the longitudinal direction, and the collecting container includes a container body having a receiving space for accommodating the collecting rod, Wherein the cover is provided with a through hole formed to penetrate the sampling rod and a discharge port to collect and / or dispense the sample using the sample collection device, Contacting the portion with a biological sample; Inserting the sampling rod into the container body including the reagent; Cutting the sampling rod by breaking the cutting groove formed in the sampling rod by using the cut-out portion; Removing the cut sampling rod; And discharging the mixture by inverting the container body.

In one embodiment, the method of sampling a sample using the sample collecting apparatus according to the present invention may further include the step of providing the sample collecting apparatus to a predetermined inspection apparatus for detection and analysis of analytes in the sample.

The sample collecting apparatus according to the present invention can be kept in a state of being accommodated in the receiving space without removing the collecting rod housed in the receiving space of the container body and eliminating the operation of removing the collecting rod. Therefore, various tests of the sample using the sample collecting apparatus according to the present invention can be performed easily.

In addition, the sample collection method using the sample collection apparatus according to the present invention can be used for an experiment without elaborating a biological specimen after analysis, In particular, convenience in an automated analyzer can be improved, and problems such as contamination of the sample that may occur during sample preparation can be minimized by shortening the inspection time.

1 is a perspective view showing a sampling rod and a sampling container of a sample collection device according to an embodiment of the present invention, respectively.
FIGS. 2A and 2B are cross-sectional views illustrating the structure of a sampling container of a sample collecting apparatus according to an embodiment of the present invention.
FIG. 3 is a cross-sectional view illustrating a state where a sampling rod of a sample collection device according to an embodiment of the present invention is inserted into a sampling container.
FIGS. 4 to 11 are perspective views showing the structure of a sampling container according to various embodiments of the present invention, respectively.
12 is a cross-sectional view showing the insertion structure of the sampling container and the sampling rod of the sample collection device according to the embodiment of the present invention.
FIG. 13 is a view illustrating a procedure of inserting a sampling rod of a sample collection device according to an embodiment of the present invention into a sampling container.
FIGS. 14A and 14B are diagrams sequentially illustrating sampling methods of a sample using the sample collecting apparatus according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. The embodiments are illustrative and not intended to limit the invention in any way.

Spatially relative terms such as "below ", " rear "," upper ", "upper ", and the like, are used to facilitate a correlation between one member or components and other components or components, Spatially relative terms should be understood in terms of the directions including the directions shown in the drawings or the directions including the different directions of the elements at the time of use or operation. For example, A member described as "below" or "below" another member may be placed "above" another member. Thus, an exemplary term "below" may include both the up and down directions The members can also be oriented in different directions, so that spatially relative terms can be interpreted according to the orientation. For example, " transverse direction " can also be interpreted as " And it shall not be limited thereto.

The thickness and size of each member in the drawings are exaggerated, omitted, or schematically shown for convenience and clarity of explanation. Also, the size and area of each component do not entirely reflect actual size or area.

Further, in the embodiments, the angles and directions mentioned in the process of describing the structure of the present invention are based on those shown in the drawings. In the description of the structure constituting the present invention in the specification, reference points and positional relationship with respect to angles are not explicitly referred to, reference is made to the relevant drawings.

FIG. 1 is a view showing a sampling rod and a sampling container of a sample collection device according to an embodiment of the present invention, FIG. 2 is a view showing a structure of a sampling container of a sample collection device according to an embodiment of the present invention, FIG. 3 is a view showing a state where a sampling rod of a sampling device according to an embodiment of the present invention is inserted into a sampling container, and FIGS. 4 to 11 are views each showing the structure of a sampling container according to various embodiments of the present invention .

A sampling device (1) according to the present invention comprises: a sampling rod (100) extending to a predetermined length and having a sampling part (120) at one end in the longitudinal direction; And a collecting container (200) in which the collecting rod (100) is accommodated; Wherein the sampling rod includes at least one cutting groove formed in the longitudinal direction and the sampling vessel includes a container having a receiving space for accommodating the sampling rod, And a cutout portion for cutting the sampling rod 100 inserted into the accommodating space 212 through the cutting groove 130 so as to be accommodated in the accommodating space 212. [

The sampling rod 100 includes a bar 110, which is a so-called swab, extending in the longitudinal direction, and a collecting part 120 provided at one end of the stick 110 do.

The stick 110 can be made of a variety of materials and various hardnesses (e.g., flexible or non-flexible). For example, the harvesting rods can be made of a thermoplastic material including metal, wood or polycarbonate, polyacrylic, polyamide, polytetrafluoroethylene, polystyrene, polypropylene and polyethylenes. Preferably, it may be made of a material having appropriate rigidity so as to be easily cut as described later.

The stick 110 is provided at one end thereof with a collecting part 120. The sampling unit 120 is formed of materials, sizes, and structures that are easy to collect, collect, and / or elute biological samples. For example, the sampling unit 120 is made of a material that is easy to collect the sample and discharge the collected sample to the reagent. For example, natural, regenerated or synthetic fibers may be used, including, but not limited to, cellulosic rayon fibers, or cotton. As another example, a closed cell foam may be used. The shape is not particularly limited, but it can take a shape such as a sphere, an ellipse, or a rectangle which is easy to collect.

The sampling rod 100 includes at least one cutting groove 130 formed along the longitudinal direction. For example, the cut groove 130 may be formed in an intermediate region of the stick 110. Here, the term " middle area " means an area within a predetermined distance to the right and left of the center of the stick 110, and is not limited to a specific length. It is sufficient that the portion cut by the cutting groove 130 can be cut through contact with a cut portion to be described later at a suitable position to be accommodated in the receiving space 212 of the picking container 200 Do. That is, the cutting groove 130 may be formed at a boundary with the sampling unit 120 or at a position spaced apart from the sampling unit 120 by a predetermined distance, Is preferably formed at the site. When two or more cutting grooves 130 are included, they may be formed at the same or different intervals.

The sampling vessel 200 is configured to contain a predetermined reagent and / or a sample, and at least a part of the sampling rod 100 can be received.

The collecting container 200 includes a container body 210 having a predetermined receiving space 212 in which the collecting bar 100 is received. Preferably, the container body 210 may have a cylindrical shape as shown in FIG. 1, but is not limited thereto. When the sampling rod 100 is received in the container body 210, the sampling rod 100 is accommodated in the container body 210 by a length corresponding to the depth or diagonal length of the accommodation space 212 And is exposed to the outside by a length exceeding the height of the container body 210. When the accommodating space 212 is divided by a partition wall described later, the collecting rod 100 can be accommodated in any of the divided spaces.

The container body 210 herein may contain reagents necessary for the analysis of biological samples. The biological sample or sample refers to a substance containing an analyte and is a fluid substance similar to a liquid or a liquid such as a solid tissue / cell of each species, blood, saliva, urine, sweat, hairs or substances extracted therefrom Or materials collected from the surrounding environment (e.g., air, soil, water, etc.). Examples include, but are not limited to, blood, urine, saliva and the like, and the blood may be whole blood, plasma, serum or whole blood, plasma, and serum in which a predetermined treatment (for example, have. Extracts of tissues or cells are selected, for example, from carbohydrates, lipids, nucleic acids, proteins, and the like. Such an analyte includes, but is not limited to, a pathogenic microorganism such as E. coli, pneumococcal bacteria, or the like, or a virus such as an influenza virus.

As used herein, the term analyte or analyte is a compound to be analyzed in a sample, which is also referred to as a target and includes proteins and nucleic acids. Proteins herein include polypeptides and peptides. The term "nucleic acid" as used herein also includes chemically synthesized DNA and RNA, including genomic DNA, cDNA, and oligonucleotides. Also, the nucleic acid may comprise a single strand or double strand, and the genomic DNA, cDNA, and oligonucleotide and RNA may be prepared or synthesized according to known methods in the art.

As used herein, the term reagent is a substance suitable for the assay, including detection and analysis of the above-described analytes, depending on the specific analyte, for example, the reagent may be reacted with various substances in the body composition, A predetermined antibody, etc., and the present invention is not limited thereto.

The container body 210 herein may be made of chemically stable synthetic resin to accommodate reagents and / or samples, and combinations thereof. But are not limited to, polyethylene, polypropylene, polystyrene, polyethyleneterephthalide, polyamide, polyester, polyvinyl chloride, polyurethane, polycarbonate, polyvinylidene chloride, polytetrafluoromethyllin, Can be produced using known molding methods using various thermosetting and thermoplastic plastics such as platinum, platinum, rhodium, platinum, rhodium, platinum, rhodium, rhodium, rhodium, rhodium, rhodium, rhodium, rhodium, rhodium, rhodium and rhodium. However, the present invention is not limited thereto, and any material suitable for the purpose of the present apparatus can be used.

The container body 210 herein may be manufactured using various known molding methods such as, but not limited to, injection, rotation, extrusion, and / or calendering, depending on the type of material. According to one embodiment, an ABS resin (Acrylonitrile, Butadiene, Styrene) or a transparent material is produced by injection molding acrylic. Those skilled in the art will be able to select certain materials and molding methods from a variety of known materials and molding methods, in order to produce a container according to the present invention. In addition, various additives such as fillers, plasticizers, stabilizers, coloring agents, antistatic agents, and the like may be used as needed in addition to the above-described synthetic resins to produce devices suitable for the purpose of the present invention.

The sampling container 200 includes a cutout portion that allows the sampling rod 100 inserted in the receiving space 212 to be cut through the cutting groove 130 and to be received in the receiving space 212. [ That is, a sampling rod 100 inserted into the container body 210 is cut through the cut groove 130 and a portion accommodated in the container body 210 is accommodated in the accommodation space 212, The cutting unit 200 includes a cutout portion for cutting the cutting groove 130 so that the picking rod 100 and the picking unit 120 provided at the lower end of the picking rod 100 can be easily received .

In this embodiment, the cut-out portion is not limited to a predetermined member or a predetermined structure for facilitating the cutting of the collecting bar 100 through the cut groove 130. That is, when a predetermined member or structure is provided in the collecting container 200 and contributes to cutting of the collecting bar 100, the member or structure may be configured as a cut-out portion, Or more. For example, the cut-out portion may be a cover 220 and a through-hole 230 provided at an upper portion of the container body 210, and a partition 240 provided in the accommodation space 212, Not limited.

The collecting container 200 may include a cover 220 provided at an upper portion of the container body 210 and a through hole 220 formed at the cover 220 and penetrating the collecting bar 100 230).

2A and 2B, the sampling container 200 has a structure in which a receiving space 212 formed in the container body 210 is opened upward, and a cover (not shown) covering the receiving space 212, The cover 220 is formed with a through hole 230 through which the sampling rod 100 is inserted.

A part of the sampling rod 100 can be inserted into the receiving space 212 through the through hole 230. The through-hole 230 may have a size and shape suitable for insertion of the sampling rod 100, and may be formed of a circular hole or a through-hole having a predetermined area, but the present invention is not limited thereto.

In this embodiment, a portion of the side of the collecting bar 100 is tangent to a portion where the cutting groove 130 is formed in contact with the through hole 230, so that the collecting bar 100 can be cut and accommodated. 2A and 2B, an external force is applied to the sampling rod 100 in a state where the sampling rod 100 is inserted into the receiving space 212 through the through hole 230, The harvesting rods 100 are bent and cut through the grooves 130 so that the harvesting portions 120 provided below the cutting grooves 130 of the harvesting rods 100 can be received in the receiving space 212 have. Accordingly, it can be understood that the through-hole 230 constitutes a part of the cut-out portion, which facilitates cutting of the collecting bar 100.

According to the above configuration, the collection unit 120 can be maintained in the state of being accommodated in the accommodation space 212 without removing the collection rod 100 contained in the accommodation space 212 separately, Inspection can be performed. Therefore, various tests of the sample using the sample collecting apparatus 1 according to the present invention can be carried out easily.

The sampling container 200 may further include a partition wall 240 disposed in the accommodation space 212 and dividing the accommodation space 212. The through hole 230 may be formed in the collection container 200, The lower end of the inserted sampling rod 100 is folded in a state of being in contact with the partition wall 240 or the inner circumferential surface of the container body 210 so that the sampling rod 100 is cut and the sampling unit 120 is accommodated do.

The partition 240 is a member disposed in the accommodation space 212 of the container main body 210 to divide the accommodation space 212 into several spaces and preferably the center of the accommodation space 212 Across the entire length or a part of the length of the container body, but the present invention is not limited thereto, and its arrangement and position are not limited. For example, the partition 240 may have a wall-like configuration that largely divides the accommodation space 212 as shown in FIGS. 3 to 11, and as another example, the partition 240 may be formed as shown in FIG. 7 And may be configured as a structure disposed at the bottom of the accommodation space 212 and having a predetermined height.

The lower end of the collecting bar 100 is connected to the partition wall 240 when the collecting bar 100 inserted into the receiving space 212 is cut and cut. The cutting can be performed by breaking. That is, when the sampling rod 100 inserted into the accommodation space 212 is folded, the lower end of the sampling rod 100 contacts the partition wall 240, so that the tilting angle of the sampling rod 100 is reduced, The cutting can be done. Of course, it is also possible that the lower end of the sampling rod 100 is bent and cut in contact with the inner circumferential surface of the container body 210, but is not limited thereto. Accordingly, as the partition wall 240 is constructed as described above, the sampling rod 100 can be more easily bent and cut. At this time, it is preferable that the partition wall 240 extends vertically so as to achieve the above-mentioned effect, and divides the accommodation space 212 into a plurality of spaces on a horizontal plane.

In one embodiment of the present invention, the partition 240 is configured such that at least a portion thereof is penetrated to allow the accommodation spaces 212 divided by the partition 240 to communicate with each other.

That is, at least a portion of the partition 240 may be provided with a predetermined flow passage 242 through which the divided accommodation spaces 212 are communicated with each other. The flow port 242 may be formed at a lower end of the partition 240, but it may be formed in various shapes, sizes, and numbers of flow ports 242. [

For example, as shown in FIGS. 4A and 4B, the flow port 242 has a configuration in which a flow port 242 such as a through window or a slit is formed in the middle portion along the longitudinal direction of the container body in the partition wall 240 Alternatively, as shown in FIGS. 6A and 6B, a predetermined penetrating slit is formed at the lower end of the partition 240 so that the flow port 242 is configured as a predetermined penetrating window.

5A and 5B, the partition wall 240 may be formed in an upper portion of the accommodation space 212 so that the divided accommodation spaces 212 are communicated with each other through a lower portion of the partition 240, And can be configured to have a gap with a bottom surface at a predetermined interval. That is, the bottom of the partition wall 240 is spaced apart from the bottom of the accommodation space 212 by a predetermined distance so that the accommodation space 212 divided by the partition wall 240 is formed at the bottom of the partition wall 240 And can be configured to be distributed to each other through the spacing space. At this time, the spacing space formed at the bottom of the partition 240 can be understood as a form of the flow port 242 described above.

A predetermined structure for limiting the displacement of the sampling rod 100 may be provided on the inner peripheral surface of the container body 210 in addition to the partition wall 240. For example, as shown in FIGS. 4B, 5B, 6B, and 7, a predetermined position restricting portion 216 is provided to limit the excessive displacement of the sampling rod 100 by the position restricting portion, The sampling rod 100 can be easily cut and accommodated. For example, as shown in FIG. 7, the position restricting portion 216 may include two walls having a predetermined height, and may have a configuration in which the collecting bar 100 can be positioned between the two walls. .

In another embodiment, the inner circumferential surface of the container body 210 may be formed with a fixing groove 244 for fixing both side ends of the partition 240 to be fixed.

8 to 11, a predetermined fixing groove 244 is formed on the inner circumferential surface of the container body 210 and both side ends of the partition 240 are inserted into the fixing groove 244, The fixing between the partition wall 240 and the container body 210 can be achieved. Accordingly, the partition 240 can be selectively disposed in the container body 210, so that the appropriate partition 240 can be selectively installed in the container body 210 according to the purpose of the sample and the type of the sample. So that work can be done in accordance with the purpose.

The partition wall 240 is configured to be detachable from the container body 210 together with the cover 220 when the container body includes the cover. In this case, the partition may be integrally formed with the cover.

9 and 11, an upper end of the partition wall 240 is connected to a lower portion of the cover 220, and the cover 220 is detachable from the container body 210 And the partition 240 and the cover 220 may be detachable from the container body 210. Meanwhile, as shown in FIGS. 10 and 11, the through-hole 230 through which the sampling rod 100 passes may be formed as a penetrating portion having a predetermined area, but is not limited thereto.

The collecting container 200 may further include a discharge port 240 provided in the cover 220. The discharge port 240 and the through hole 230 may be formed by the partition 240 And are formed at positions corresponding to the divided accommodation spaces 212, respectively.

That is, as shown in FIG. 1, a predetermined discharge port 240 for discharging the sample filled in the container body 210 to the outside can be formed on the cover 220. At this time, the part where the discharge port 240 is formed and the part where the through hole 230 is formed can be divided by the partition 240. That is, the discharge port 240 and the through hole 230 may be formed with the partition 240 interposed therebetween.

Accordingly, it is possible to prevent the sample from leaking unnecessarily by the through hole 230 when discharging the sample filled in the container body 210 through the discharge port 240.

The through hole 230 may have a smaller outer diameter than the receiving space 212 in the upward direction and the sampling rod 100 may be formed in the through hole 230 And at least one portion in the longitudinal direction corresponding to the outer diameter of the through-hole 230 is configured to have a conical configuration so as to seal the through-hole 230 by being fitted.

That is, the through-hole 230 may have a structure suitable for being hermetically sealed by the sampling rod 100. 9, the through-hole 230 has a conical or conical shape with an inner diameter becoming smaller toward the upper side, and at least a portion of the through- As shown in Fig.

12, the through-hole 230 has a conical shape or a conical shape with an inner diameter becoming smaller toward the upper side, and at least a portion of the stick 110 of the collecting bar 100 may have a shape A conical or conical shape corresponding to the shape of the through hole 230 may be formed to seal the through hole 230.

At this time, the insertion of the collecting bar 100 may be performed, for example, by placing the collecting bar 100 in the receiving space 212 and then covering the cover 220. 113, after the collecting bar 100 is positioned in the through hole 230, the collecting bar 100 is inserted into the through hole 230, By covering the container body 210, sealing of the through hole 230 by the sampling rod 100 can be ensured.

According to this configuration, when the sample in the receiving space 212 is discharged to the outside through the discharge port 240, the sealing of the through hole 230 is secured, thereby preventing unnecessary leakage through the through hole 230 .

Hereinafter, a method of collecting a sample using the sample collecting apparatus according to an embodiment of the present invention will be described.

FIGS. 14A and 14B are views sequentially illustrating sampling methods of a sample using the sample collecting apparatus according to an embodiment of the present invention, but the sampling apparatus and order are not limited thereto.

A method of collecting a sample using the sample collecting apparatus (1) according to the present invention comprises the steps of: contacting the collecting unit (120) formed in the collecting rod (100) with a biological sample; Inserting the sampling rod 100 into the container body 210 including the reagent; Cutting the sampling rod (100) by breaking the cutting groove (130) formed in the sampling rod (100) by using the cut portion; And removing the cut-off collecting rod (100).

First, the sampling rod 100 is immersed in a liquid or solid sample, and a sample is sampled using the sampling unit 120. The sample includes, but is not limited to, a mucous sample such as a stool, a sputum, a needle, a runny nose, or a liquid sample such as plasma, serum, whole blood.

Next, as shown in (a), the sampling rod 100 from which the sample is collected is inserted into the container body 210. At this time, the reagent necessary for detecting the analyte in the liquid sample may be contained in the container body 210, or the reagent may be added after the sample 120 is taken in.

As shown in FIG. 5B, the cut-off portion 130 formed in the collecting bar 100 is broken by using the cut-out portion to cut the collecting bar 100. When the through hole 230 or the partition 240 is provided in the cover 220 as described above, the cutout portion has a structure including a through hole and a partition, The sheath 100 can be broken in the cutting groove 130 by shearing stress applied to the cutting groove by bending the end of the exposed sampling rod 100 after inserting the sampling rod 100 through the opening. Thereafter, when the exposed portion of the cut sampling rod 100 is removed, the sampling rod 100 under the cutting groove 130 remains inside the container body 210. Meanwhile, in this process, the sample collected in the sampling unit 120 is mixed with the reagent in the container body 210. That is, in the above process, the reagent and the sample are mixed with each other, and the container body may be shaken before inserting the sampling rod and / or after cutting the sampling rod to facilitate mixing with the reagent.

When the sampling container 200 is turned upside down, the reaction product between the sample and the reagent in the container body 210 can be discharged through the discharge port 250 as shown in FIG. At this time, since the through hole 230 is sealed by the sampling rod 100, unnecessary leakage of reagent and sample reactant by the through hole 230 can be prevented.

A sampling method using a sample collection apparatus according to another embodiment of the present invention will be described.

A sampling method using the sampling device (1) according to the present invention includes: a sampling rod extending to have a predetermined length and having a collecting part at one end in the longitudinal direction; And a sampling container in which the sampling rod is accommodated; Wherein the collecting rod includes at least one cutting groove formed along the longitudinal direction, the collecting container including a container body having a receiving space for accommodating the collecting rod, a cover provided on the container body, Wherein the cover includes a through hole formed to penetrate the sampling rod and a discharge port, wherein the discharge port and the through hole are formed in an accommodating space divided by the partition wall A method of collecting a sample using a sample collection apparatus, the method comprising the steps of: bringing the sampling unit formed in the sampling rod into contact with a biological sample; Inserting the sampling rod into the container body including the reagent; Cutting the sampling rod by breaking the cutting groove formed in the sampling rod by using the cut-out portion; Removing the cut sampling rod; And discharging the mixture by inverting the container body. In this process, the reagent and the sample may be mixed with each other, and the container may be shaken before inserting the sampling rod and / or after cutting the sampling rod to facilitate mixing with the reagent.

Meanwhile, the sample collecting apparatus 1 according to the present invention including the reaction product of the sample and the reagent may be delivered to a predetermined inspection apparatus for detecting the analyte in the reaction product. That is, the result of the reaction between the sample and the reagent filled in the container body 210 of the sample collecting apparatus 1 according to the present invention is partially or entirely collected through a predetermined apparatus provided in the testing apparatus, . From this point of view, the sampling device 1 of the present application can be used for sampling and / or pretreatment of biological samples for various analyzes.

For example, a predetermined inspection equipment may be an automated equipment capable of distributing reaction products and detecting an analyte in a reaction product, and the automated equipment may include means for distributing or distributing reaction products.

The method according to the present invention facilitates the use of an automated analyzer, especially after the biological sample is collected, which can be eluted in the container without any after-treatment process eluted by the operator's work, The shortening of the time can minimize the problems such as contamination of the sample which may occur during the preparation of the sample.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, It should be understood that various modifications may be made by those skilled in the art without departing from the spirit and scope of the invention.

1: Sampling device
100:
110: Stick
120:
130: Cutting groove
200: collection container
210:
212: accommodation space
216:
220: cover
230: Through hole
240:
242: Distributor
244: Fixing groove
250: Outlet

Claims (13)

A collecting rod extending to have a predetermined length and provided with a collecting part at one end in the longitudinal direction; And a sampling container in which the sampling rod is accommodated; / RTI >
Wherein the harvesting rod includes at least one cutting groove along the longitudinal direction,
Wherein the sampling container includes a container body having a receiving space for receiving the sampling rod and a cut-out portion for cutting the sampling rod inserted in the receiving space into the receiving space so as to be accommodated in the receiving space.
The method according to claim 1,
Wherein the sampling container comprises:
A cover provided on an upper portion of the container body, and a through hole formed in the cover,
Wherein the cut-out portion includes the through-hole,
Wherein a portion of the sampling rod inserted into the receiving space through the through hole is bent in a state where a part of the side of the sampling rod is in contact with the through hole to cut and accommodate the sampling rod Collecting device.
3. The method according to claim 1 or 2,
Wherein the sampling container comprises:
Further comprising partition walls disposed in the accommodating space and dividing the accommodating space, all or part of which are formed along the longitudinal direction of the container body,
Wherein the partition wall includes a flow port configured to allow at least a part of the partition wall to penetrate so that the divided accommodation spaces are communicated with each other,
Wherein the cut-out portion includes the partition wall,
Wherein the sampling rod is cut and accommodated by bending the lower end of the sampling rod inserted into the vessel body in a state in contact with the partition wall or the inner circumferential surface of the vessel body.
The method of claim 3,
Further comprising a position restricting portion on an inner circumferential surface of the container main body, the position restricting portion being located at a position facing the partition wall.
The method of claim 3,
The partition may be configured to have a gap with a predetermined distance from the bottom surface of the accommodation space so that the divided accommodation spaces can communicate with each other through a lower portion of the partition, To the sample collection device.
The method of claim 3,
Wherein the partition wall is detachable from the container body,
And a cover connected to a lower portion of the cover and detachable from the container body together with the cover when the cover is included.
The method of claim 3,
On the inner peripheral surface of the container body,
And a fixing groove for fixing both side ends of the partition wall to each other.
3. The method of claim 2,
Wherein the sampling container comprises:
Further comprising a discharge port provided in the cover. The method according to claim 2 or 3,
Wherein the sampling container comprises:
Further comprising a discharge port provided in the cover,
Wherein the discharge port and the through hole are respectively formed at positions corresponding to the accommodating space divided by the partition wall when the partition wall is included.
3. The method of claim 2,
The through-
The outer diameter of which becomes smaller toward the upward direction from the accommodation space,
The sampling rod
And at least one portion in the longitudinal direction corresponding to the through hole is fitted in the through hole so as to close the through hole and has a conical configuration.
A method for sampling a sample using a sample collection device,
A collecting rod extending to have a predetermined length and provided with a collecting part at one end in the longitudinal direction; And a sampling container in which the sampling rod is accommodated; / RTI >
Wherein the harvesting rod includes at least one cutting groove along the longitudinal direction,
Wherein the sampling container includes a container body having a receiving space for receiving the sampling rod and a cut-out portion including a cut-out portion that is cut in the cutting groove and received in the receiving space, As a method of collecting a sample,
Contacting the harvesting part formed on the harvesting rod with a biological sample; Inserting the sampling rod into the container body including the reagent; Cutting the sampling rod by breaking the cutting groove formed in the sampling rod by using the cut-out portion; And removing the cut sampling rod.
A method for sampling and dispensing a sample using the sample collecting apparatus according to claim 9,
Contacting the harvesting part formed on the harvesting rod with a biological sample; Inserting the sampling rod into the container body including the reagent, mixing the reagent and the sample;
Cutting the sampling rod by breaking the cutting groove formed in the sampling rod by using the cut-out portion;
Removing the cut sampling rod; And
And discharging the mixture by inversing the container body.
13. The method according to claim 11 or 12,
And providing the sample collection device to a predetermined inspection equipment.

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