KR20150027890A - Composition comprising herbal mixture extract for treating or preventing thyroid cancer - Google Patents

Abstract

The present invention relates to a composition including an extract of a mixture of Astragalus membranaceus, Angelica gigas and Trichosanthes kirilowii as an active ingredient for treating or preventing the thyroid cancer. The extract of a mixture of Astragalus membranaceus, Angelica gigas and Trichosanthes kirilowiimixture according to the present invention has an anti-cancer effect of specifically inhibiting the proliferation of thyroid cancer cells without affecting the growth of the normal cells, and has an excellent effect of suppressing the transfer and invasiveness of thyroid cancer cells, thereby being able to be efficiently used for preventing or treating the thyroid cancer.

Description

[0001] The present invention relates to a composition for preventing or treating thyroid cancer, comprising a mixed herbal extract as an active ingredient,

The present invention relates to a composition for preventing or treating thyroid cancer, which comprises a mixed extract of Hwanggi, Angelica gigas and Angelica keiskei koidz. As an active ingredient.

Cancer is one of the incurable diseases that humanity needs to solve. In the world, huge capital is invested in development to heal it globally. In Korea, it is the first disease among the causes of death, It is diagnosed and more than 60,000 people are dying. Smoking, ultraviolet rays, chemicals, food, and other environmental factors are among the carcinogens that cause cancer, but the development of therapeutic agents is difficult due to the various causes, and the effects of the therapeutic agents are also different depending on the site where they occur. Currently, substances used as therapeutic agents have considerable toxicity. Since they can not selectively remove only cancer cells, it is urgently necessary to develop a less toxic and effective anticancer drug to prevent the cancer from occurring after cancer development .

Thyroid carcinoma is the most common malignancy of the endocrine system. It is the most common malignant tumor of the endocrine system, including papillary carcinoma, follicular carcinoma, Hurthle cell neoplasm, Anaplastic carcinoma, Medullary thyroid carcinoma, thyroid carcinoma). Of these, differentiated tumors, such as papillary thyroid carcinoma and thyroid follicular carcinoma, have a very good prognosis, but survival rates are dramatically lowered when invading into surrounding tissue or metastasizing to other organs. Reverse-forming cancer is a rare malignant undifferentiated cancer, and most of the patients die within 6 months. These reversed-type cancers generally do not respond to radioactive iodine therapy, chemotherapy, and other therapies.

On the other hand, Astragalus membranaceus is a perennial herb with rosemary roots of dicotyledonous plants, distributed in Korea, Japan, Manchuria, Northeastern China, and eastern Siberia. It is often said that it is better to plant it as a herb, and to take it out in the autumn in one shot, to remove the outermost root and rootstock, and to dry it in the sun, and to use it without peeling it. It is prescribed for the weakness of the body, fatigue bloating, cold sweat and so on because it has the efficacy such as the tonic, the jihan, the diuretic, the exorcism.

Angelica gigas is a dried perennial roots of Angelica gigas , a perennial paste that is very sweet and very warm. The efficacy of Angelica gigas has a blood-producing effect when blood is lacking. Angelica promotes blood flow in the coronary arteries and stimulates erythropoiesis.

Trichosanthes kirilowii Maximowicz refers to the perennial root of the perennial belonging to the genus (Cucurbitaceae) or the root of the yellow topolith. There is no smell, the taste is spicy and the quality is cold. Treating petechiae, boils, and nausea when heat causes damage to the juice. It mainly produces lungs and stomach fever, so it helps to quench thirst and softens the body. .

As described above, various pharmacological effects of Hwanggi, Angelica gigas, and Chrysanthemum have been known. However, the anticancer effect of the mixed extracts on thyroid cancer has not been elucidated at all.

Accordingly, the present inventors have continued efforts to develop an anticancer agent against a novel thyroid cancer. As a result, the present inventors have found that a mixed extract of Hwanggi, Angelica gigaspora, and Angelica keiskei does not affect the growth of normal cells but specifically inhibits proliferation of thyroid cancer cells And that it has an excellent effect of inhibiting the mobility and invasiveness of thyroid cancer cells, thereby completing the present invention.

It is an object of the present invention to provide a pharmaceutical composition for the prevention or treatment of thyroid cancer, which comprises a mixed extract of Hwanggi, Angelica gigas and Chrysanthemum as an active ingredient.

Yet another object of the present invention is to provide a food composition for preventing or ameliorating thyroid cancer, which comprises a mixed extract of Hwanggi, Angelica gigas and Angelica keiskei koidz. As an active ingredient.

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing or treating thyroid cancer, which comprises a mixed extract of Hwanggi, Angelica gigas and Angelica keiskei koidz. As an active ingredient.

The present invention also provides a food composition for preventing or ameliorating thyroid cancer, which comprises a mixed extract of Hwanggi, Angelica gigas and Angelica keiskei koidz. As an active ingredient.

According to the present invention, the mixed extract of Hwanggi, Angelica gigas and Angelica keiskei has an anticancer effect that specifically inhibits the proliferation of thyroid cancer cells without affecting the growth of normal cells, and is superior in inhibiting the metastasis and invasiveness of thyroid cancer cells And thus can be usefully used for the prevention or treatment of thyroid cancer.

FIG. 1 shows the effect of the mixed extract of the present invention on the proliferation of normal cells (RIE cells) through MTT assay.
FIG. 2 is a graph showing the effect of the mixed extract of the present invention on the proliferation of SNU-790 cells, a thyroid papillomavirus cancer cell line (Hwanggi: Angelicae: Chunghwa = 1: 1: 1) through MTT assay.
FIG. 3 is a graph showing the effect of the mixed extract of the present invention (Hwanggi: Angelica: Chunghwa = 3: 1: 1) on the proliferation of SNU-790 cells as a thyroid papillary cancer cell through MTT assay.
FIG. 4 is a graph showing the effect of the mixed extract of the present invention (Hwanggi: Angelica gigas Nakai: ginseng: ginseng = 1: 1: 1: 1) on the proliferation of SNU-790 cells as a thyroid papillary cancer cell through MTT assay.
FIG. 5 is a graph showing the effect of the mixed extract of the present invention (Hwanggi: Angelica gigas Nakai: ginseng = 3: 1: 1: 1) on the proliferation of SNU-790 cells as a thyroid papillary cancer cell through MTT assay.
FIG. 6 is an MTT assay showing the effect of the mixed extract of the present invention (Hwanggi: Angelicae: chrysanthemum = 1: 1: 1) on the proliferation of SNU-80 cells which are inversely formed thyroid cancer cells.
FIG. 7 is a graph showing the effect of the mixed extract of the present invention (Hwanggi: Angelicae: Chrysanthemum = 3: 1: 1) on the proliferation of SNU-80 cells which are inversely formed thyroid cancer cells through MTT assay.
FIG. 8 is a graph showing the effect of the mixed extract of the present invention on the proliferation of SNU-80 cells, which are inversely formed thyroid cancer cells (Angelica: Angelica: Angelicae: ginseng = 1: 1: 1: 1) through MTT assay.
FIG. 9 is a graph showing the effect of the mixed extract of the present invention (Hwanggi: Angelica gigas Nakai: ginseng = ginseng = 3: 1: 1: 1) on the proliferation of SNU-80 cells which are inversely formed thyroid cancer cells through MTT assay.
10 is a graph showing the effect of the mixed extract of the present invention on the transformation of SNU-80 cells, which are inversely formed thyroid cancer cells.
FIG. 11 is a graph showing the effect of the mixed extract of the present invention on the infiltration of SNU-80 cells, which are inversely formed thyroid cancer cells.

The present invention provides a composition for preventing or treating thyroid cancer, which comprises a mixed extract of Hwanggi, Angelica gigas and Angelica keiskei koidz. As an active ingredient.

The composition comprises a pharmaceutical composition or a food composition.

Hereinafter, the present invention will be described in more detail.

A mixed extract of Hwanggi, Angelica gigas and Angelica keiskei koidz. As active ingredients in the composition of the present invention can be obtained by the following method.

First, Hwanggi, Angelica gigas and Chrysanthemum are washed with water to remove foreign matter. Hwanggi, Angelica gigas and Chrysanthemum can be cultivated or marketed without limitation. After mixing the hwanggi, angelica, and silkworm, the mixture is completely immersed in a solvent of 10 to 30 times the volume. The mixing ratio of the herbal medicine is preferably a weight ratio of Hwanggi: Angelica: Angelite = 1 to 3: 1: 1. The extraction method can be impregnated or warmed at room temperature. The extraction solvent is not limited thereto, but one or more solvents selected from water, an alcohol having 1 to 4 carbon atoms and a mixed solvent thereof may be used, preferably ethanol, more preferably 20 to 40% (v / v) ethanol. The extract was filtered and concentrated under reduced pressure to obtain a final mixed extract of Huangji, Angelica gigas and Angelica keiskei koidz.

The composition may further comprise a ginseng extract. When the ginseng is further contained, the mixing ratio of the herbal medicine is preferably a weight ratio of Hwanggi: Angelica: Angelica ginseng: 1: 3: 1: 1: 1.

According to the present invention, the mixed extract of Hwanggi, Angelica gigas and Angelica keiskei has an anticancer effect that specifically inhibits the proliferation of thyroid cancer cells without affecting the growth of normal cells, and is superior in inhibiting the metastasis and invasiveness of thyroid cancer cells And thus can be usefully used for the prevention or treatment of thyroid cancer.

The thyroid cancer includes papillary carcinoma, follicular carcinoma, Hurthle cell neoplasm, anaplastic carcinoma, undifferentiated thyroid carcinoma, and medullary thyroid carcinoma. .

The composition of the present invention may further contain at least one known active ingredient having anticancer activity against thyroid cancer together with a mixed extract of Hwanggi, Angelica gigas, and Chunghwa powder.

The compositions of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions. In addition, it can be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, oral preparations such as syrups and aerosols, external preparations, suppositories and sterilized injection solutions according to a conventional method. Suitable formulations known in the art are preferably those as disclosed in Remington ' s Pharmaceutical Science, recently, Mack Publishing Company, Easton PA. Examples of carriers, excipients and diluents that can be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose , Microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When the composition is formulated, it is prepared using a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, or an excipient usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose, lactose, Gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and suspension include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The term "administering" as used herein is meant to provide any desired composition of the invention to a subject in any suitable manner.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and body weight of the individual, the degree of disease, the drug form, the route of administration and the period of time, and can be appropriately selected by those skilled in the art. For the desired effect, the mixed extract of the present invention may be administered in an amount of 1 mg / kg to 10000 mg / kg per day, and may be administered once or several times a day.

The pharmaceutical composition of the present invention may be administered to a subject in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine dural or intracerebral injection.

The composition of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy and biological response modifiers for the prevention or treatment of thyroid cancer.

In the present invention, the health functional food refers to a food having a biological control function such as prevention or improvement of disease, bio-defense, immunity, recovery after disease, suppression of aging, and should be harmless to human body when taken over a long period of time. The mixed extract of Hwanggi, Angelica gigas, and Angelica gigas Nakai of the present invention can be added to health functional foods for the purpose of preventing or improving thyroid cancer. When the mixed extract of Hwanggi, Angelica gigas and Chrysanthemum flowers of the present invention is used as a food additive, the mixed extract of Hwanggi, Angelica gigas and Chrysanthemum can be used as it is or can be used together with other food or food ingredients, . The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, in the production of food or beverage, the mixed extract of Yellow, Angelica and Celadon fractions of the present invention is added in an amount of 15% by weight or less, preferably 10% by weight or less based on the raw material. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

There is no particular limitation on the kind of the food. Examples of foods to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen and other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. The natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, natural sweeteners such as dextrin and cyclodextrin, synthetic sweeteners such as saccharine and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 10 g, preferably about 0.01 to 0.1 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in a carbonated beverage, and the like. In addition, the composition of the present invention may comprise flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. Although the ratio of such additives is not critical, it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred embodiments, experimental examples, and production examples are provided to facilitate understanding of the present invention. However, the following examples, experimental examples and production examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples, experimental examples and production examples.

Example 1. Preparation of mixed extracts of Hwanggi,

(1) 1: 1: 1 or (2) 3: 1: 1 weight ratio (w / w) of astragalus membranaceus , Am, Angelica gigas , Ag or Trichosanthes kirilowii Maximowicz, ), And the mixture was put into an extractor, and 8 ~ 10 times of 30% (v / v) ethanol was added thereto, followed by extraction for 2 ~ 3 hours. The extract solution was filtered, and the filtrate was concentrated under reduced pressure and then dried to obtain a mixed extract of Hwanggi, Angelica gigas and Angelica keiskei (the average yield of mixed extracts mixed at a weight ratio of 1: 1: 1 was about 35.5%, 3: 1: The average yield of mixed extracts mixed at a weight ratio of about 29.85%).

Example 2. Preparation of mixed extracts of Hwanggi, Angelica, Angelica, and Ginseng

(1) 1: 1: 1: 1 or (2) 3: 1: 1: 1 at a weight ratio (w / w) After adding 30% ethanol, it was extracted for 2 to 3 hours. The extract solution was filtered, and the filtrate was concentrated under reduced pressure and dried to obtain a mixed extract of Hwanggi, Angelica gigas, Chrysanthemum ginseng and Ginseng (average yield of mixed extracts mixed at a weight ratio of 1: 1: 1: : Average yield of mixed extracts mixed at a weight ratio of 1: 1: 1 of about 24.24%).

Experimental Example 1. Effect of the mixed extract of the present invention on the growth of normal cells

In order to confirm the effect of the mixed extracts prepared in Examples 1 and 2 on the growth of normal cells, MTT assays were performed according to conventionally known methods using RIE normal intestinal epithelial cells (RIEs) . More specifically, the RIEs cell line cultured on a 96-well plate was treated with various concentrations (50-500 μg / ml) of the mixed extracts of Hwanggi, Angelica gigas, ml), and then cultured at 37 ° C and 5% CO ₂ for 48 hours. Each cell was treated with MTT (3- (4,5-Dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) reagent and incubated for additional 4 hours. The supernatant was removed and 100 μl of DMSO (dimethyl sulfoxide) was added. The absorbance was finally measured at a wavelength of 590 nm, and the absorbance value was compared with the control group in which no substance was treated. The results are shown in Fig.

As shown in Fig. 1, the extracts of Hwanggi, Ganoderma lucidum, and Chrysanthemum powder inhibited the growth of RIEs cells, which are normal cells, while the mixed extracts of Hwanggi, Had no effect on the growth of RIEs, which are normal cells. The above results show that the mixed extract of Hwanggi, Angelica gigas and Chrysanthemum and the mixed extracts of Hwanggi, Angelica gigas, Chrysanthemum and Ginseng improve the negative effects (cytotoxicity against normal cells) Respectively.

Experimental Example 2. Effect of the mixed extract of the present invention on the growth of thyroid cancer cells

To examine the effect of the mixed extracts prepared in Examples 1 and 2 on the growth of thyroid cancer cells, an MTT assay was carried out in the same manner as in Experimental Example 1 above. SNU-790 cell line, human papillary thyroid cancer cells, and SNU-80 cell line, human anaplastic thyroid cancer cells, were used as cancer cell lines.

The results are shown in Figs. 2 to 9.

As shown in FIG. 2 and FIG. 3, the mixed extract of the present invention, in which the ratio of Hwanggi, Angelica and Angelica keiskei at a weight ratio of 1: 1: 1 or 3: 1: 1, 1: 1: 1 or 3: 1: 1: 1 of the present invention, as shown in FIGS. 4 and 5, The SNP-790 cells were cultured in the same manner as the thyroid papilloma cells.

6 and 7, the mixed extract of Hwanggi, Angelica gigas and Chrysanthemum fractions of the present invention in a weight ratio of 1: 1: 1 or 3: 1: 1 according to the present invention is an SNU- 8 and 9, it was confirmed that the herbaceous plants of the present invention, Angelica japonica, Angelica keiskei and Ginseng were mixed in a ratio of 1: 1: 1: 1 or 3: 1: 1 : 1 in the ratio of SNU-80 cells to the SNU-80 cells.

Experimental Example 3. Effect of the mixed extract of the present invention on the transfer of thyroid cancer cells

Wound healing assays were performed to verify the effect of the mixed extracts prepared in Examples 1 and 2 on the transfer of thyroid cancer cells. More specifically, SNU-80 cells were seeded on a 6-well plate to be 350,000 cells per well, and then cultured overnight. When the cells form a monolayer and are 80-90% full, they are scratched across the cells using a pipette tip to create intercellular spacing. Then, the cells were washed with PBS, and then treated with the mixed extracts of Hwanggi, Angelica gigas and Acanthopanacophora prepared in Examples 1 and 2 or the mixed extracts of Hwanggi, Angelica, Angelica and Ginseng (50 ㎍ / ㎖). After 24 hours, the intercellular space was narrowed (40 × magnification) compared with the control group. The results are shown in Fig.

As shown in FIG. 10, it was confirmed that the mixed extracts of Hwanggi, Angelica gigas and Chrysanthemums of the present invention and the mixed extracts of Hwanggi, Angelica gigas, Chrysanthemum ginseng and Ginseng had excellent effect of reducing the transfer ratio of thyroid cancer cells.

Experimental Example 4 Effect of the Mixed Extract of the Present Invention on Infiltration of Thyroid Cancer Cells

In order to examine the effect of the mixed extracts prepared in Examples 1 and 2 on the invasion of thyroid cancer cells, an invasion assay was performed according to a conventionally known method. More specifically, SNU-80 cells were cultured in an upper chamber coated with Matrigel in advance, and then mixed extracts of Hwanggi, Angelica gigas, and Chunghwa granules prepared in Examples 1 and 2, (50 mu g / ml). After 24 hours, the cells were washed with PBS or water, fixed overnight at 4 ° C with 4% paraformaldehyde, and stained with hematoxylin and eosin (H & E). The results are shown in Fig.

As shown in FIG. 11, it was confirmed that the mixed extracts of Hwanggi, Angelica gigas and Chrysanthemums of the present invention and the mixed extract of Hwanggi, Angelica gigas, Chrysanthemum and Ginseng significantly inhibited the invasion of thyroid cancer cells.

Hereinafter, the pharmaceutical composition of the present invention and the preparation example of the food composition will be described, but the present invention is not intended to be limited but is specifically described .

Formulation Example 1. Preparation of a pharmaceutical composition

1-1. Manufacture of Powder

20 mg of mixed extract of Hwanggi, Angelica and Angelica

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

1-2. Manufacture of tablets

Mixture of Hwanggi, Angelica and Angelicae

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

1-3. Preparation of capsules

Mixture of Hwanggi, Angelica and Angelicae

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

1-4. Injection preparation

Mixture of Hwanggi, Angelica and Angelicae

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO _4? 2H ₂ O 26 mg

(2 ml) per 1 ampoule in accordance with the usual injection preparation method.

1-5. Manufacture of liquid agent

20 mg of mixed extract of Hwanggi, Angelica and Angelica

10 g per isomer

5 g mannitol

Purified water . Suitable amount

Each component was added and dissolved in purified water according to the usual liquid preparation method, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was added with purified water to adjust the total volume to 100 ml, And sterilized to prepare a liquid preparation.

Formulation Example 2. Preparation of Food Composition

2-1. Manufacture of health food

100 mg of mixed extract of Hwanggi, Angelica and Chrysanthemum

Vitamin mixture quantity

Vitamin A acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

0.15 mg of vitamin B2

Vitamin B6 0.5 mg

Vitamin B12 0.2 μg

Vitamin C 10 mg

Biotin 10 μg

Nicotinic acid amide 1.7 mg

Folic acid 50 μg

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

2-2. Manufacture of health drinks

100 mg of mixed extract of Hwanggi, Angelica and Chrysanthemum

Vitamin C 15 g

Vitamin E (powder) 100 g

19.75 g of ferrous lactate

3.5 g of zinc oxide

Nicotinic acid amide 3.5 g

Vitamin A 0.2 g

Vitamin B1 0.25 g

Vitamin B2 0.3 g

Water quantification

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 liter container, It is used in the production of the health beverage composition of the invention.

Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

Claims (11)

A pharmaceutical composition for the prophylaxis or treatment of thyroid cancer, which comprises a mixed extract of Hwanggi, Angelica gigas and Angelica keiskei koidz. As an active ingredient. The method according to claim 1, wherein the mixed extract is obtained by extracting a mixture of Hwanggi, Angelica gigas, and Chunghwa, with at least one solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms and a mixed solvent thereof. Or a pharmaceutically acceptable salt thereof. The pharmaceutical composition according to claim 2, wherein the alcohol having 1 to 4 carbon atoms is 20 to 40% (v / v) ethanol. [Claim 2] The pharmaceutical composition according to claim 1, wherein the mixed extract of Hwanggi, Angelica gigas and Angelica keiskei are mixed at a weight ratio of Hwanggi: Angelica: Angelica: 1: 3: 1: 1. The pharmaceutical composition according to claim 1, wherein the composition further comprises a ginseng extract. The pharmaceutical composition for preventing or treating cancer of thyroid according to claim 5, wherein the composition further comprising the ginseng extract is mixed at a weight ratio of Hwanggi: Angelica: Angelica: ginseng = 1: 3: 1: 1: Composition. The method of claim 1, wherein the thyroid cancer is selected from the group consisting of Papillary carcinoma, Follicular carcinoma, Hurthle cell neoplasm, Anaplastic carcinoma and Medullary thyroid cancer. carcinoma). < RTI ID = 0.0 > 21. < / RTI > A food composition for preventing or ameliorating thyroid cancer, comprising a mixed extract of Hwanggi, Angelica gigas and Angelica keiskei koidz. As an active ingredient. [9] The food composition according to claim 8, wherein the mixed extract of Hwanggi, Angelica gigas and Angelica keiskei are mixed at a weight ratio of Hwanggi: Angelica: Angelica: 1: 3: 1: 1. 9. The food composition according to claim 8, wherein the composition further comprises a ginseng extract. The composition for preventing or improving cancer of thyroid according to claim 10, wherein the composition further comprising the ginseng extract is mixed at a weight ratio of Hwanggi: Angelica: Angelica: ginseng = 1: 3: 1: 1: .

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