KR20150017206A

KR20150017206A - Fermentation metabolite of Dendropanax morbiferus produced by solid-state fermentation and manufacturaring process for the same

Info

Publication number: KR20150017206A
Application number: KR1020130093202A
Authority: KR
Inventors: 조창수
Original assignee: 조창수
Priority date: 2013-08-06
Filing date: 2013-08-06
Publication date: 2015-02-16

Abstract

The present invention relates to a Dendropanax morbiferus fermentation metabolite produced through solid fermentation and a method for producing the same. The method for producing a Dendropanax morbiferus fermentation metabolite through solid fermentation according to an aspect of the present invention comprises: a solid fermentation substrate producing step of washing, drying, and powdering one or more of a Dendropanax morbiferus leaf and a Dendropanax morbiferus stem; a microorganism pre-culturing step of purely culturing fermentation microorganisms, wherein the present step is selectively performed at the same time with, before, or after the solid fermentation substrate producing step; a solid fermenting step of producing a Dendropanax morbiferus fermented liquid sequentially by inoculating 5 to 15% of the microorganism purely cultured liquid into the solid fermentation substrate, by adjusting the water content thereof to 30 to 60%, maintaining the relative humidity thereof at 78 to 82%, and fermenting the same at 25 to 30°C for 5 to 20 days; and a step of obtaining a Dendropanax morbiferus fermentation metabolite by sterilizing, extracting, filtering, and concentrating the Dendropanax morbiferus fermented liquid obtained from the solid fermenting step.

Description

FIELD OF THE INVENTION The present invention relates to a fermented fermented metabolite of fermentation by solid fermentation and a fermentation metabolite produced by the same,

FIELD OF THE INVENTION The present invention relates to a sulfuric acid fermentation metabolite by solid fermentation and a preparation method thereof, and more particularly to a sulfuric acid fermentation metabolite by solid fermentation with improved bioavailability and physiological activity, and a preparation method thereof.

In the modern society, chemical synthesis technology has been used for industrial development. However, these chemical synthesis technologies have caused serious environmental problems by using high temperature, high pressure, highly toxic reagents and solutions. .

Biotransformation refers to the production of a desired product from a precursor using the physiological function of a biological organism. The term biotransformation refers to bioprocessing, bioconversion, biosythesis, biocatalysis, etc. As well. Bio-conversion technology is used in many fields because it has less energy consumption and environment-friendly than chemical synthesis technology. As a result, food, medicine, and cosmetics are used in many fields. 50% of the total.

Among the biotransformation technologies, researches have been conducted on methods of increasing biologic activity by converting glycosides into aglycons through microorganism-based methods and enzyme conversion.

Fermentation using microorganisms can be expected to increase the absorption of the active substance as a result of the low molecular weight of the active ingredient, and to exhibit the same effect normally to a person who does not have a specific intestinal bacteria.

Research through biotransformation techniques has been conducted in many places, mainly through the saponins of ginseng. Among them, ginseng contains saponin called gisenoside. Research has been conducted to develop a method for increasing the yield of compound K, which is a strong physiologically active ingredient. Studies on the possibility of developing functional food materials using bioconversion technology for flavonoids, which are known to have excellent antioxidant activity, and studies on inhibition of melanin synthesis by using biotransformation technology have been conducted. And so on.

Research on the development of food materials is progressing gradually through bioconversion technology.

On the other hand, Hwangchil is an evergreen tree of the dicotyledonous plant, and its international name is Dendropanax morbifera, which means panacea. It is also called a golden tree. It is a Korean native species native to the southern coast of Korea and Jeju Island. Its height is about 15m. The flower is bloomed at the end of June branch. The fruit is elliptical nucleus with a length of 7 ~ 19㎜. It ripens in color. The constituents and effects of this woody wood are mainly sesquiterpene, which contains water, gum, alcohol, ester and the like. Hwangchil, which is a sap extracted from Hwangchilgi bark, is said to have been used only by the royal family because it has a remarkable pharmacological effect and a delicate environment.

It has been widely accepted that nutritional value, pharmacological value, and therapeutic value have been studied in modern scientific studies and it has been studied in various directions and forms in order to utilize useful materials of Huangchil. Korean Patent Laid-Open Publication No. 2004-0107852 discloses a method for preparing a Huangchil extract having a skin whitening effect and a Huangchil extract according to the method, Korean Patent Registration No. 10-0318019 discloses a Huangchil extract having anticancer activity, 0663284 discloses a seedling extract of Hwangchil, which is excellent in physiological activity. Korean Patent Registration No. 10-0988072 discloses a fermented product of Hwangchulnam and a pharmaceutical composition containing the same, Korean Patent Publication No. 2003-0005098 discloses a fermented product And Korean Unexamined Patent Application Publication No. 2005-0036093 discloses a technique of an ultraviolet cut cosmetic composition containing an extract of Hwangjak tree as an active ingredient.

An object of one embodiment according to the present invention is to provide a fermented yellowish fermented metabolite by solid fermentation with improved bioavailability and physiological activity and a method for producing the same.

One embodiment of the present invention relates to a solid fermentation substrate preparation step of washing, drying and pulverizing at least one of yellowish leaves or stalks; A step of pre-culturing the microorganism, which is selectively performed at the same time as or before or after the step of producing the solid fermentation substrate, wherein the fermenting microorganism is purely cultured; The solid fermentation substrate is inoculated with 5 to 15% of the microorganism pure culture medium, the moisture content is adjusted to 30 to 60%, the relative humidity is maintained at 78 to 82%, and fermentation is performed at 25 to 30 DEG C for 5 to 20 days A solid fermentation step of producing a sulfuric acid fermentation broth; And a step of sterilizing, extracting, filtering and concentrating the fermented fermentation broth obtained in the solid fermentation step to obtain a fermented fermented fermented body, and a method for producing fermented fermented fermented body by solid fermentation.

In the solid fermentation substrate preparation step, the litter leaf or stem may be pulverized to a size of 100 to 1000 탆.

In the solid fermentation substrate preparation step, the burdock leaf or stem may be powdered and sterilized.

The microorganism is an edible mold Aspergillus oryzae , mushroom strain Phellinus baumii , or mushroom strain Ganoderma It can be lucidum .

The microorganism pre-culturing step may be carried out at 25 to 37 DEG C for 2 to 10 days.

In the solid fermentation step, the juice may be further mixed with the solid fermentation substrate.

The solid content of the juice may be 1 to 10 Brix.

The juice may be mixed in an amount of 10 to 30% by weight based on the solid fermentation substrate.

The sulfuric acid fermented metabolite may be processed in the form of a concentrate or powder.

Another embodiment of the present invention provides a yellowish fermented metabolite produced by the above method.

The sulfuric acid fermented metabolite may be used in a cosmetic composition, a pharmaceutical composition or a food composition.

The fermented fermented metabolic material by solid fermentation according to the present invention can be understood as a state in which the useful substance of the woody spruce tree is low-molecularized by the microorganism and the secondary metabolite resulting from the fermentation into the microorganism. According to one embodiment of the present invention, the specific form of the fermented fermentation broth can be determined according to the processing method of the fermented fermented fermentation broth by solid fermentation according to the above-described method, and the fermented fermentation broth may be in the form of a concentrated liquid, May be in the form of a solid powder that has undergone a process.

The yellowish fermented metabolite according to one embodiment of the present invention is characterized by having low fermentation energy consumption and high yield as well as excellent antioxidant ability and bioavailability and physiological activity by solid fermentation I have.

The fermentation metabolic body according to one embodiment of the present invention uses a powdered solid fermentation substrate and shortens the fermentation time through pre-cultivation of the microorganism, facilitates the production by improving the yield thereof, May be prepared in the form of a concentrate or a dry powder. Accordingly, it can be changed into various types of formulations and can be used for various purposes. For example, the above sulfuric acid fermentation metabolite can be used as a cosmetic composition, a pharmaceutical composition, a food composition, and the like.

1 is a flowchart schematically showing steps of manufacturing a method of manufacturing a yellowish fermented metabolite by solid fermentation according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art. Therefore, the embodiments of the present invention can be modified into various other forms, and the scope of the present invention is not limited to the following embodiments.

Throughout the description of the present invention, when a component is referred to as " comprising ", it means that it can include other components as well, without excluding other components unless specifically stated otherwise.

In the specification of the present invention, it is to be understood that when a step is located "on" or "before" another step, this is not only the case where a step is in a direct time series relationship with another step, And may have the same rights as in the case of an indirect temporal relationship in which the temporal order of the two phases can be changed.

The terms " about "," substantially ", etc. used to the extent that they are used throughout the specification of the present invention are used in their numerical values or in close proximity to their numerical values when the manufacturing and material tolerances inherent in the meanings mentioned are presented, Is used to prevent unauthorized exploitation by an unscrupulous infringer of precise or absolute disclosures in order to aid in the understanding of the disclosure. The term " step " or " step of ~ " used throughout the specification does not mean " step for.

The present invention relates to a method for preparing a fermented yellowish fermented metabolite by solid fermentation and a method for producing the fermented fermented metabolite by solid fermentation. Microorganism pre-culture step; Solid fermentation step; And a step of obtaining a sulfuric acid fermented metabolite by the solid fermentation according to an embodiment of the present invention.

The fermented fermented metabolic material by solid fermentation according to the present invention can be understood as a state in which the useful substance of the woody spruce tree is low-molecularized by the microorganism and the secondary metabolite resulting from the fermentation into the microorganism. According to one embodiment of the present invention, the specific form of the yellowish fermented metabolite can be determined according to the processing method of the yellowish fermentation broth completed by solid fermentation according to the above-described method, and the fermentation liquid having the yellowish- Or may be in the form of a solid powder that has undergone a drying process.

The major component of Hwangchil is Sesquiterpene, which contains water, gum, alcohol and esters. It is known that Hwangchil, which is a sap extracted from Hwangchilbok tree, has a remarkable pharmacological effect and a delicate environment.

In recent years, nutritional value, pharmacological value, and therapeutic value have been recognized by the scientific research of Hwangchu. It has been reported that yellowtail is effective for many diseases such as improving blood circulation (cholesterol, blood pressure), antioxidant (anti-aging), liver function improvement (hangover, fatigue recovery, detoxification), immunity enhancement and regeneration.

However, the active ingredient of Hwigae-gil is slow in absorbing human body and has limited use in human body. There have been attempts to utilize the active ingredient of Hwangchujang in the human body. However, there has been a problem that it is difficult to process and mass-produce Hwangchilchu and by-products in large quantities due to difficulty in changing formulations in various forms.

However, according to one embodiment of the present invention, the fermented fermented metabolite is a powdered solid fermentation substrate, and the fermentation time is shortened by using the microorganism by pre-culturing, the yield is improved and the production is easy, Metabolites may be prepared in the form of concentrates or dry powders. Accordingly, it can be changed into various types of formulations and can be used for various purposes. For example, the above sulfuric acid fermentation metabolite can be used as a cosmetic composition, a pharmaceutical composition, a food composition, and the like.

When the sulfuric acid fermentation metabolite is used in a cosmetic composition, it is preferable to use a commonly used ingredient such as purified water, a moisturizer, an oil, a surfactant, a high alcohol, a thickener, a chelating agent, a colorant, a fatty acid, an antioxidant, Such as solutions, suspensions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, and the like, And spray or the like.

In addition, when used in a pharmaceutical composition, it can be formulated using a pharmaceutically acceptable carrier and / or excipient, and can be mixed with a lubricant, wetting agent, sweetening agent, flavoring agent, emulsifying agent, suspending agent, preservative and the like.

In addition, when used as a food composition, it can be mixed with components such as protein, carbohydrate, fat, nutrients, seasoning and flavor, which are conventionally added in food production, and can be formulated into various forms.

Hereinafter, each step of the method for producing a yellowish fermented metabolite by solid fermentation according to an embodiment of the present invention will be described concretely, and the yellowish fermentation metabolite by solid fermentation according to one embodiment of the present invention will be described more concretely &Lt; / RTI >

A method for preparing a fermented fermented body by solid fermentation according to an embodiment of the present invention comprises the steps of: preparing a solid fermentation substrate for washing, drying and pulverizing at least one of the leaves or the stem; A step of pre-culturing the microorganism, which is selectively performed at the same time as or before or after the step of producing the solid fermentation substrate, wherein the fermenting microorganism is purely cultured; The solid fermentation substrate is inoculated with 5 to 15% of the microorganism pure culture medium, the moisture content is adjusted to 30 to 60%, the relative humidity is maintained at 78 to 82%, and fermentation is performed at 25 to 30 DEG C for 5 to 20 days A solid fermentation step of producing a sulfuric acid fermentation broth; And a step of sterilizing, extracting, filtering and concentrating the fermented fermentation broth obtained in the solid fermentation step to obtain a fermented fermented fermented body.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flowchart schematically showing steps of manufacturing a fermented yellowish fermented metabolite by solid fermentation according to an embodiment of the present invention; FIG.

First, a solid fermentation substrate preparation step of washing, drying and pulverizing at least one of the yellowish leaves or the stem can be performed (S ₁ ).

As a step of preparing a fermentation substrate to be used for solid fermentation, it is possible to carry out a step of washing and drying the burnt leaves and the stem, followed by pulverization. The size of the litter blade and the stem powder may be 100-1000 mu m although not limited thereto. If the size of the powder is less than 100 mu m, an undesired metabolite may be formed during the fermentation process. If the size of the powder exceeds 1000 mu m, the fermentation speed and yield may decrease.

According to one embodiment of the present invention, only yellowish leaves or stalks may be used or mixed.

It may also be subjected to a sterilization procedure after being comminuted, but may be autoclaved and autoclave sterilized by high pressure steam sterilization.

In addition, a microorganism pre-culture step of selectively cultivating the fermenting microorganism purely before or after the fermentation substrate production step (S ₂ ) may be performed.

In one embodiment of the present invention, microorganisms may be cultured and then used for the fermentation of the Phellinus linteus extract. The strains used for solid fermentation were edible fungi Aspergillus oryzae , A mushroom strainPhellinus baumiiWowGanoderma lucidum Lt; / RTI > Although it is difficult to propagate strain because Huangchil has strong antimicrobial activity, it is judged that the strain has excellent proliferative ability in fermentation of Huangchil.

The microorganism may be carried out at 25 to 30 DEG C for 2 to 10 days. But are not limited to, edible fungus Aspergillus The oryzae are cultured in PDA medium at 30 ° C for 5 days, and the spores formed can be harvested with 0.1% physiological saline, then seeded again on the PDB medium, and cultured for 5 days at 30 ° C and 120 rpm.

Phellinus mycelia baumii and Ganoderma lucidum can be used as an inoculum by culturing in a PDA medium at 30 ° C for 10 days to form a mycelium, inoculating it into a mycelial PDB liquid medium, and culturing at 25 ° C for 30 days at 130 rpm for 10 days.

Next, a solid fermentation step in which the solid fermentation substrate is inoculated with the microorganism pure culture medium and cultured can be performed (S ₃ ).

According to one embodiment of the present invention, the microorganism pure culture medium is inoculated with 5 to 15%, the moisture content is adjusted to 30 to 60%, the relative humidity is maintained at 78 to 82% RTI ID = 0.0 > 20 < / RTI > days.

More specifically,Aspergillus oryzae , Phellinus baumii , Ganoderma lucidum Each pure culture medium may be 10%Aspergillus oryzaeLt; RTI ID = 0.0 > 30 C < / RTI > for 5 days,Phellinus baumii WowGanoderma lucidumCan be cultured for 20 days at 25 占 폚.

According to one embodiment of the present invention, the microbial pre-culture is performed before the fermenting microorganism is inoculated into the solid fermentation substrate, whereby the solid fermentation time can be shortened and the yield thereof can be greatly improved.

In addition, the yellowish fermented metabolite according to one embodiment of the present invention may contain various secondary metabolites depending on the kind of the microorganism, as well as the useful substance of the sulfuric acid. According to one embodiment of the present invention, it is possible to have excellent antioxidant ability in a metabolite obtained by fermenting yellowtail leaf with a mushroom.

Further, according to one embodiment of the present invention, a juice solution may be prepared, and a juice solution may further be included in the solid fermentation substrate (S ₄ ).

The juice may be provided as a source of sugar in the solid fermentation step. When the fermentation substrate contains a juice solution, the fermentation metabolite may be different from that of the fermentation substrate.

According to one embodiment of the present invention, the juice may be prepared by adding water twice to a boat and refluxing the mixture at 90 to 100 ° C for 5 hours, followed by filtration and concentration under reduced pressure (60 ° C or less). The solids content of the juice may be adjusted to 1 to 10 Brix and sterilized at 121 占 폚 for 15 minutes prior to inoculation with the microorganism.

Growth of the strain may be difficult due to the strong antimicrobial activity of Huangchil, but solid fermentation can be promoted if the juice is added to the solid fermentation substrate.

Next, the sulfuric acid fermentation broth obtained by solid fermentation is sterilized, extracted, filtered and concentrated to obtain a sulfuric acid fermented metabolite (S ₅ ).

A sulfuric acid fermentation metabolism product can be obtained from the fermented sulfuric acid fermentation broth. The According to one embodiment, when the solid fermentation is completed, A fermentation broth can be obtained, and thus the fermentation metabolism substance, which is a final product, can be easily obtained.

More specifically, the above-described fermentation broth can be sterilized at 100 to 121 ° C for 15 to 20 minutes, and then extracted with 10 times of water or 70% alcohol. When water is added, it can be extracted at 90 to 95 ° C for 10 hours, and when it is added, it can be extracted at 50 ° C for 5 hours. After extraction, it can be used as a concentrate or as a solid powder by concentration under reduced pressure at 60 ° C or lower. The concentrate may be used as a concentrate of 10 to 60 Brix, and the concentrate may be prepared as a solid powder by lyophilization and spray drying.

The sulfuric acid fermented metabolite may be used in a cosmetic composition, a pharmaceutical composition, a food composition, etc., and may be processed into a concentrate or a solid powder according to the intended use and formulation.

The fermented hydrogen peroxide metabolism is a metabolite resulting from the fermentation of microorganisms, and its ingredients and structure can not be clearly specified. However, the useful substance of sulfurylcholine can be reduced in molecular weight to increase the absorption rate in the body, Especially, it has been proved that antioxidant effect is excellent.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail with reference to the following examples, but they should not be construed as limiting the scope of the present invention.

[Example]

[Preparation of sulfuric acid fermentation metabolite]

Burrows Solid powder manufacture

After drying, the leaves and the leaves were crushed and cut into 1 mm and 1 ~ 2 mm, respectively, and autoclaved at 121 ° C for 30 to 60 minutes.

Juice Produce

Water was added to the pellet twice, and the mixture was refluxed at 90 to 100 ° C for 5 hours, filtered and concentrated under reduced pressure (60 ° C or less). The solid content was adjusted to 1, 5, and 10 Brix and sterilized at 121 ° C for 15 minutes.

Pure culture of solid fermentation microorganisms

Aspergillus oryzae were cultured for 5 days at 30 ° C and 120 rpm by inoculating the PDB medium with the spores obtained by pure culture in the PDA medium. Phellinus Baumii and Ganoderma lucidum were inoculated on PDB medium and cultured at 25 ℃ and 130 rpm for 10 days.

Microbial inoculation and solid fermentation

10% of the fermented microorganism pure culture medium was inoculated in a mixture of 20% of dried powder of yellowish crushed or dried powder of yellowish crushed and 20% of juice (10 Brix), and the water content was adjusted to 30 to 60% Aspergillus The oryzae were incubated at 30 ° C for 5 days with Phellinus baumii and Ganoderma lucidum was cultured at 25 ° C for 20 days.

Fermentation Metabolite purchase

The incubated fermented broth was sterilized at 100 to 121 ° C for 15 to 20 minutes and then extracted with water or 70% ethanol 10 times at 90 to 95 ° C for 10 hours and 50 ° C for 5 hours. The filtrate was concentrated under reduced pressure (60 DEG C or less) and processed into a concentrate and a solid powder, respectively.

[evaluation]

One. Burrows Fungi on fermentation substrate Proliferative ability Measure

In order to evaluate the growth of three strains to be used for solid fermentation, Huangchil hot water extract was prepared and its proliferative activity was investigated. The hot water extract was adjusted to have a solid content of 1, 5, 10 Brix, and 1% of the pure culture broth of each of the above strains was inoculated and cultured under the same conditions as the pure culture of each strain. The results are shown in Table 1 below, in which the degree of growth is shown as - (non-viable), + (poor growth), ++ (normal growth), and +++ (excellent growth).

Strain Concentration / Brix One 5 10 Aspergillus oryzae +++ +++ +++ Phellinus baumii +++ +++ +++ Ganoderma lucidum +++ +++ +++

As shown in Table 1, all three strains showed good growth at a high concentration of 10Brix. And Bacillus Subtilis showed better growth at 5 and 10 Brix than 1Brix. In other words, all three strains selected in this experiment were judged to be usable for the production of the yellowish fermentation metabolism by solid fermentation.

In addition, the same amount of 10Brix of juice (10 Brix) was mixed with 1, 5, and 10 Brix of Welsh hot water extract, and the growth of three strains was examined. The results are shown in Table 2 below. And the growth pattern in the yellowish hot water extract was almost the same.

2. Yellowish fermentation Metabolite Antioxidant experiment

After drying sterilized dried powder of yellowgrass leaves and powdered ash leaves, Aspergillus oryzae (Hwang Kuk Kyung), Phellinus baumii , Ganoderma lucidun (Ganoderma lucidum) and 10% of the pure culture medium of the three strains were inoculated. The Hwang Guk-gum was cultured at 30 ℃ for 5 days with shaking culture, and the mushroom bacteria was cultured at 25 ℃ for 3 days with shaking. The fermented broth was filtered, concentrated, Was used to investigate DPPH scavenging ability.

Free radical scavenging activity and measured by the reducing power of the sample on the electron donating effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH, WaKo) radicals. To 1 ml of each concentration sample, 1 ml of 500 μM DPPH solution was added and stirred, followed by reaction at 37 ° C for 30 minutes in a dark place. The absorbance of the reaction solution was measured at 517 nm, and the radical scavenging activity was calculated in the same manner as described below.

Free radical scavenging activity (%) = (1-A / B) x 100

A: sample addition absorbance, B: sample no addition absorbance

Fermentation
temperament Fermentation density
(占 퐂 / mg) DPPH Scatters (%) Control group A.
oryzae P.
baumii G.
lucidun Dried eggplant dried powder 0 0.21 0.21 0.21 0.21 50 10.75 15.75 15.75 12.63 100 21.94 31.04 31.04 25.21 250 52.75 73.89 70.41 57.25 500 76.26 89.68 89.71 89.01 750 88.50 90.14 89.78 89.43 1000 89.59 90.53 89.99 89.72 Burrows Leaf dry powder 0 0.21 0.21 0.21 0.21 50 10.78 10.27 15.95 13.76 100 21.71 22.17 28.32 24.21 250 50.28 51.35 78.01 54.78 500 75.67 87.14 88.01 84.03 750 81.29 89.14 89.63 88.59 1000 85.23 89.73 92.15 89.48

The DPPH scavenging ability of the fermented yellowtail fermented by the solid fermentation was slightly higher than that of the leaves in the control group and the activity was the highest in the metabolized fermented with the mushroom.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but many variations and modifications may be made without departing from the scope of the present invention. It is evident that many variations are possible by those skilled in the art. It will be apparent to those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. something to do.

Claims

A step of preparing a solid fermentation substrate for washing, drying and pulverizing at least one of yellowish leaves or stalks;
A step of pre-culturing the microorganism, which is selectively performed at the same time as or before or after the step of producing the solid fermentation substrate, wherein the fermenting microorganism is purely cultured;
The solid fermentation substrate is inoculated with 5 to 15% of the microorganism pure culture medium, the moisture content is adjusted to 30 to 60%, the relative humidity is maintained at 78 to 82%, and fermentation is performed at 25 to 30 DEG C for 5 to 20 days A solid fermentation step of producing a sulfuric acid fermentation broth; And
Sterilizing, extracting, filtering and concentrating the fermentation broth obtained in the solid fermentation step to obtain a fermented fermentation broth;
&Lt; / RTI > wherein the method comprises the steps of: preparing a fermented yellowish fermented metabolite by solid fermentation;

The method according to claim 1,
Wherein the litter leaf or stem is pulverized to a size of 100 to 1000 mu m in the step of preparing the solid fermentation substrate.

The method according to claim 1,
Wherein the litter leaf or stem is powdered and sterilized in the step of preparing the solid fermentation substrate.

The method according to claim 1,
The microorganism is an edible mold Aspergillus oryzae , mushroom strain Phellinus baumii , or mushroom strain Ganoderma lucidum . < RTI ID = 0.0 > 11. < / RTI >

The method according to claim 1,
Wherein the microorganism pre-culture step is performed at 25 to 37 DEG C for 2 to 10 days.

The method according to claim 1,
Wherein the solid fermentation step further comprises adding a juice to the solid fermentation substrate in the solid fermentation step.

The method according to claim 6,
Wherein the solid content of the juice is 1 to 10 Brix. &Lt; RTI ID = 0.0 > 8. < / RTI >

The method according to claim 6,
Wherein the juice is mixed in an amount of 10 to 30% by weight with respect to the solid fermentation substrate.

The method according to claim 1,
Wherein the sulfuric acid fermented metabolite is processed in the form of a concentrate or powder.

10. A yellowish fermented metabolite produced according to any one of claims 1 to 9.

The sulfuric acid fermentation metabolite according to claim 10, which is used in a cosmetic composition, a pharmaceutical composition or a food composition.