KR20150009898A - A composition for cosmetics including extract of culture media of human adipose tissue-derived stem cells - Google Patents

Abstract

The present invention relates to a composition which is effective in blocking UV, alleviating wrinkle, whitening, maintaining skin elasticity, and preventing aging, and to cosmetics comprising the same. According to the present invention, the composition contains 3-7 wt% of a culture medium of human adipose tissue-derived stem cells and a concentrate thereof.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition containing a human adult adipose stem cell culture extract and a cosmetic composition using the same. [0002]

The present invention relates to a composition containing a culture concentrate of human adipose-derived stem cells having an effect of blocking ultraviolet rays, improving wrinkles and whitening within a predetermined range, and a cosmetic using the same.

With the recent improvements in medical technology and public health benefits, an aging society is rapidly emerging. As a result, the quality of life is being improved and the desire for the maintenance of youth is increasing. Much research is underway in various areas to delay and prevent appearance, especially the aging that is first manifested in skin.

The skin consists of the epidermis, the dermis and the subcutaneous tissue. The outer skin of the skin consists of a stratified squamous epithelium, which acts as a protective barrier against the external environment. The epidermis is divided from the outside into stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum and stratum basale. The dermis is a layer between the epidermis and the subcutaneous tissue. It is divided into papillary dermis and reticular dermis. The dermis is composed of blood vessels, collagen, elastin fibers, pores, lipids, sebaceous glands, Sensory nerves, fibroblasts, and macrophages, and they occupy the largest portion of the skin.

The dermis consists mainly of collagen and elastin fibers, which play a role in supporting the skin. Therefore, when such a dermis is troubled, wrinkles occur and skin elasticity is lost and the skin ages. Collagen is known to play the most important role in skin regeneration, skin moisture content, wound healing and wrinkle improvement, and is produced from fibroblasts. Collagen has a function to contain a large amount of water, and serves to supply moisture to the dermis. When aging occurs, the collagen's water-containing function is deteriorated and wrinkles are increased. Collagen also causes wound healing by filling the wound with continuous collagen production of fibroblasts when a wound is developed.

Stem cells are cells that have the ability to differentiate into all kinds of cells that make up the body, such as nerves, blood, There are two ways to obtain such stem cells. First, stem cells from embryos derived from embryos (embryonic stem cells), and stem cells from adult parts of the body (adult stem cells ). Although there are functional differences, embryonic stem cells and adult stem cells all have the ability to differentiate into different types of cells. Although embryonic stem cells have excellent differentiation potential and long telomeres, they have an ethical problem and it is difficult to acquire a large number of cells. However, adult stem cells can obtain a large number of cells, There is a drawback that the risk of infection or the ability to differentiate is relatively low.

 Adult stem cells are very safe for medical applications. Specifically, cancer does not occur even when transplanted into the body for long-term regeneration. Since the immune rejection does not occur because it is in the adult body, autologous transplantation using its own cells is possible. In addition, there is a site-specific differentiation that differentiates itself according to the characteristics of the surrounding tissues. Since it does not cause cancer even if it is injected in undifferentiated state, besides producing cells necessary immediately after transplantation, It has the advantage of self-renewal ability to regenerate and store necessary undifferentiated stem cells. Therefore, the importance of adult stem cells has recently been highlighted, and various studies are under way to reveal its usefulness.

The present invention relates to a composition containing a culture concentrate of human adipose-derived stem cells having an effect of blocking ultraviolet rays, improving wrinkles and whitening within a predetermined range, and a cosmetic using the same.

The present invention can provide a skin elasticity retention or anti-aging composition containing 3 to 7% by weight of a human adipose-derived stem cell culture liquid or a concentrate thereof.

In the composition according to the present invention and cosmetics using the same, effects of ultraviolet ray blocking, wrinkle reduction, whitening, skin elasticity maintenance and anti-aging can be obtained.

In order to accomplish the object of the present invention, the present invention provides a method for culturing human adipose-derived stem cells in a serum-free medium, culturing the medium in a serum-free medium, filtering the stem cell culture solution or concentrate thereof to 3 to 7% The present invention provides a composition for protecting against ultraviolet rays, improving wrinkles, and whitening.

 Preferably, the stem cell culture solution or concentrate thereof comprises (a) subculturing human adipose-derived stem cells in a serum medium and then continuously culturing in serum-free medium; (b) applying at least one physical stimulus selected from hypoxic culture, ultraviolet irradiation, low-frequency treatment, nutrient deficiency, and mechanical friction to the cultured stem cells; (c) adding one or more vitamins selected from vitamin A, vitamin B, vitamin C and vitamin D to a culture medium, and (d) culturing the culture.

 In the composition of the present invention, the serum-free medium may preferably be a mixed medium of DMEM (Dulbecco's Modified Eagle's Medium) and Ham's F-12 nutrient mixture (refer to Korean Patent Publication No. 2008-0109725) The mixing ratio may preferably be 1: 1 for DMEM and Ham's F-12 nutrient mixture.

 The composition of the present invention can exhibit skin elasticity retention or anti-aging effect by inhibiting the activity of elastase.

 The stem cells are undifferentiated cells capable of differentiating into various types of tissue cells, and they are separated from embryonic stem cells and adult tissues isolated from inner cell mass of blastocyst. (Adult stem cells) can be roughly classified into. Adult stem cells are undifferentiated cells having a mutipotency derived from a mammal including a human, preferably a human adult tissue. Examples of the adult stem cells include various cells such as bone marrow, blood, brain, skin, Can be derived from adult cells.

 The human adipose-derived stem cells are a kind of adult stem cells isolated from human adipose tissue. The acquisition of adipose tissue can be obtained incidentally during the liposuction process, which is usually performed, and thus it is possible to easily obtain and cultivate a sufficient amount of stem cells, and also has superior safety compared to bone marrow harvesting.

 The human adipose-derived stem cells can be cultured by a conventional method using a medium for stem cell culture, for example, a serum medium. Preferably, human adipose-derived stem cells are cultured in DMEM (Dulbecco's Modified Eagle's Medium) containing 10% fetal bovine serum, and finally cultured in a 1: 1 mixed medium of serum-free DMEM and Ham's F-12 nutrient mixture , The protein content in the obtained culture liquid can be increased. The serum-free medium stage minimizes differentiation by exposing stem cells isolated from adipose tissue to a specific extreme environment, and enables the recovery of the proteins secreted by the stem cells from the culture medium as much as possible.

 The present invention also provides a UV-blocking, wrinkle-improving and whitening method, characterized in that the composition is topically applied or injected intradermally into the skin. The dosage form is preferably, but not limited to, topical application or intradermal injection.

 The present invention also provides a therapeutic agent for ultraviolet ray blocking, wrinkle reduction and whitening comprising the composition as an active ingredient.

 The pharmaceutically acceptable carrier to be contained in the therapeutic agent for skin elasticity retention or aging prevention of the present invention is conventionally used in the present invention and includes lactose, dextrose, sucrose, sorbitol, starch, gelatin, calcium silicate, polyvinylpyrrolidone But are not limited to, water, cellulose, water, syrup, methylcellulose, niacinamide, retinoid, melanocin, octocrylene, ethylcysteic salicylic acid, zinc oxide and the like.

The therapeutic agent for ultraviolet screening, wrinkle reduction and whitening of the present invention may be formulated using pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by those having ordinary skill in the art to which the present invention pertains Or may be prepared by penetration into a multi-dose container, and may additionally include a dispersant or stabilizer.

 The present invention also provides cosmetics for ultraviolet screening, wrinkle improvement and whitening comprising the composition as an active ingredient.

 The components contained in the cosmetics for ultraviolet screening, wrinkle reduction and whitening of the present invention may contain, as an active ingredient, components commonly used in cosmetic compositions in addition to the stem cell culture concentrate, and examples thereof include antioxidants, stabilizers, , Vitamins, pigments and flavoring agents, and carriers.

 In addition, the UV-blocking, wrinkle-improving, and whitening cosmetic compositions of the present invention may further contain ingredients for conventional skin elasticity maintenance or anti-aging other than the above-mentioned stem cell culture concentrate, It can be easily selected and used.

Example 1 Preparation of Cosmetic Composition Containing Extract of Human Fatty-Derived Stem Cell Culture Liquid According to the Present Invention

Using the human adipose stem cell culture extract, a cosmetic composition having an ultraviolet blocking, wrinkle-improving and whitening effect can be prepared with the composition shown in Table 1 below.

ingredient Unit: wt% Delta-tocopherol 0.5 Human adipose-derived stem cell culture fluid 5.0 Cetostearyl alcohol 2.0 Glyceryl stearate 1.5 Microcrystalline 0.7 Squalane 5.0 Liquid paraffin 3.0 Trioctanoin 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Cyclomachicone 3.0 Tocopheryl acetate 0.2 BHT 0.05 Ethyl hexylmethoxycinnamate 5.0 Retinol 3.0 Ethylhexylsalicylic acid 2.0 Octocrylene 2.0 Purified water Balance Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Xanthan gum 0.1 EDTA-2Na 0.05 Incense, preservative Suitable amount system 100

Here, ethylhexylmethoxycinnamate may be contained in an amount of 3 to 7% by weight.

Here, a composition containing 1 to 5% by weight of retinol may be prepared.

Here, ethylhexylsalicylic acid may be contained in an amount of 1 to 3% by weight.

Here, the content of octocrylene may be 1 to 3% by weight.

Claims (2)

A composition for maintaining skin elasticity or preventing aging, which contains 3 to 7% by weight of a human adipose-derived stem cell culture liquid or a concentrate thereof. A cosmetic product made using the composition according to claim 1.