KR20140141872A - Composition comprising germinated soybean peptides for anti-hypertension - Google Patents
Composition comprising germinated soybean peptides for anti-hypertension Download PDFInfo
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- KR20140141872A KR20140141872A KR20130063036A KR20130063036A KR20140141872A KR 20140141872 A KR20140141872 A KR 20140141872A KR 20130063036 A KR20130063036 A KR 20130063036A KR 20130063036 A KR20130063036 A KR 20130063036A KR 20140141872 A KR20140141872 A KR 20140141872A
- Authority
- KR
- South Korea
- Prior art keywords
- hypertension
- germinated soybean
- germinated
- acid
- peptides
- Prior art date
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- A23L11/05—Mashed or comminuted pulses or legumes; Products made therefrom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- Y10S530/80—Antihypertensive peptides
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Abstract
The present invention relates to a composition for preventing or treating hypertension comprising germinated soybean peptides as an active ingredient.
The germinated soybean peptides according to the present invention exhibit excellent ACE inhibitory activity and are effective not only for prevention, improvement or treatment of hypertension but also for human safety as a natural material and thus have a very high utilization value.
Description
The present invention relates to a composition for preventing or treating hypertension comprising germinated soybean peptides as an active ingredient.
Hypertension is one of the most common diseases of modern geriatric diseases. The causes of essential hypertension, which accounts for the majority of hypertension, have not been elucidated yet. However, it is presumed to be mainly caused by the following two mechanisms. The first mechanism is that the sympathetic nervous system is activated to cause a change in the pressure-diuresis, which is the mechanism by which the renin-angiotensin system is involved. The second mechanism is that abnormalities occur prior to Na-releasing hormone and Na-transport, leading to the release of Na (see, for example, Jang et al. (Kettle, TW et al., N. Engl., J. Med., ≪ RTI ID = 0.0 > In addition to these abnormalities of plasma membranes, Na and Ca concentrations are increased and hypertensive symptoms are manifested by the mechanism of elevated arterial pressure (Ljungman, 5, Hypertension, 3: 318 (1981) It is.
On the other hand, an angiotensin converting enzyme (Kininase II, peptidyldipeptide hydrolase, EC 3.4.15.1 (hereinafter referred to simply as 'ACE') hydrolyzes the C-terminal dipeptide (His-Leu) of inactive angiotensin I, It may convert to angiotensin II, which has the action of smooth muscle contraction of blood vessel walls, and may inhibit the release of water and Na by promoting aldosterone secretion in the adrenal cortex. In addition, bradykinin with vasodilatory action is degraded and inactivated (Manjusri, D. and Richard, LS, J. Biol. Chem., 250: 6162 (1975); Davis, JO, Fed. Proc 36: 1753 (1977): Hall, JE et al., J. Hypertens., 4: 387 (1986)). The activity of ACE is causing hypertension.
For this reason, specific inhibitors of ACE are useful for controlling blood pressure in the human body, and ACE activity is closely related to hypertension development, so in vitro inhibition of ACE has been used for screening antihypertensive agents. It has been reported that ACE inhibitors such as captopril, enalapril, and ramipril are synthesized as therapeutic vasoconstrictors, but the side effects caused by the use of these drugs have been reported to include taste disorders, leukocytopenia, angioedema, and liver dysfunction. (Elsevier, Amsterdam, Netherlands, Vol. 5, pp. 246-271, 1984). In addition, there is a continuing need for screening of antihypertensive agents.
As a result of this side effect due to pharmacotherapy, there is a growing interest in antihypertensive natural substances using food materials. It has been reported that hydrolysates such as milk, eggs, chicken, pork, beef, fish, beans, corn and microalgae have antihypertensive efficacy.
Accordingly, it is an object of the present invention to provide a composition for preventing, improving and treating hypertension, which includes pharmacological and physiological activities for hypertension and has no adverse effects on the human body.
As means for solving the above problems, the present invention provides a composition for preventing, improving and treating hypertension comprising germinated soybean peptides as an active ingredient.
The soybean consists of 35-40% of protein, 15-20% of fat, 35% of carbohydrate and 5% of other fat, and isoflavone, oligosaccharide, saponin, lecithin, , Lutein, and unsaturated fatty acids, fibrin, vitamins and minerals. In particular, unsaturated fatty acids contain linoleic acid (linoleic acid) 52% and linolenic acid (11%) in large amounts and are known as excellent sources of essential fatty acids. The physiological and pharmacological activities of soybean have been reported to be improved, such as anti-aging, osteoporosis, breast cancer, cervical cancer prevention, cardiovascular disease and hypertension. Especially, the hydrolyzate of soybean protein is known as a peptide showing ACE inhibitory activity based on low toxicity and high safety, and is useful for hypertension. There is a case in which the peptide showing ACE inhibitory activity is isolated from the hydrolyzate of soybean protein (Japanese Patent Laid-Open No. 3-1671981). In addition, Yu et al., 1996, which demonstrated that soybean hydrolysates exhibit ACE inhibitory activity in vascular tissues in vivo and lower systolic blood pressure in congenital hypertensive rats, (J. Korean Soc. Food Sci. Nutr. 25, 1031-1036).
In the germination process, nutrient changes such as qualitative changes of storage proteins, increase of micronutrients, increase of digestibility of constitutional carbohydrates in the body and decrease of the reflecting quantitative factors are carried out in the germination process. In the germination process, It is known that the water absorption is improved and the permeability is improved. Thus, researches on the application to various functional food materials have been actively conducted. In the present invention, germinated soybean peptides are used as an effective ingredient instead of soybean hydrolyzate.
On the other hand, the present inventors have completed the present invention by confirming that germinated soybean peptides exhibit excellent ACE inhibitory activity in the process of extracting substances having pharmacological and physiological activities of hypertension from natural products. As can be seen from the following examples, the germinated group had better ACE inhibitory effect than the non-germinated group, and the germinated soybean peptides had better ACE inhibitory effect than the normal germinated soybean extract, and the positive control group, It can be seen that ACE inhibition similar to prill is exhibited.
In the present invention, the germinating soybean peptide comprises (a) germinating soybeans; (b) obtaining a germinated soybean extract from the germinated soybean; And (c) separating the germinated soybean peptides from the germinated soybean extract (see FIG. 1).
The method of germinating the soybeans in step (a) may be according to a conventional method. However, the present invention is not limited to this, but the soybeans are immersed in water in a state where the temperature and humidity are maintained. Preferably, the immersion is carried out by dipping in water at a temperature of 18 to 35 캜 for 24 to 72 hours, sprinkling gently in a dark state in which the temperature is maintained at 20 to 35 캜 and the relative humidity is maintained at 50 to 100%. The intermittent spraying may be done by spraying at intervals of 4 to 6 hours for 1 to 10 minutes, and cultivation is convenient when a device equipped with a timer is used. The germination is preferably germinated until the bud of the bean grows to 1 to 3 cm. The soybean is applicable to any seed of a plant belonging to the soybean family.
The method of obtaining the germinated soybean extract of step (b) may be according to a conventional method. But it is not limited thereto, and can be obtained by pulverizing the germinated soybean after drying and heat treatment, and immersing it in water or an organic solvent. Preferably, the germinated soybean extract may be a hot-water extract.
The method of obtaining the germinated soybean peptide of step (c) may be according to a conventional method. But is not limited to, can be obtained by treating with ordinary proteolytic enzymes. As can be seen from the examples, the germinated soybean peptides obtained by treating the supernatant obtained by centrifuging the germinated soybean extract of step (b) above with proteolytic enzymes were obtained from germinated soybean peptides obtained by centrifuging after proteolytic enzyme treatment ACE activity inhibition was better. Therefore, it is preferable to prepare by treating the proteolytic enzyme after centrifugation, drying with hot air and pulverizing.
The term " hypertension " in the present invention refers to essential hypertension, and the cause of the hypertension has not yet been accurately determined. To date, what is known as the physiochemical mechanism of hypertension is the angiotensin converting enzyme (ACE), which causes physiological effects such as vasoconstriction and reabsorption of sodium in the kidney, and body fluid regulation.
The sprout peptides according to the present invention are highly effective for hypertension by inhibiting the action of angiotensin converting enzyme (ACE) in the small intestine.
As one embodiment of the present invention, the present invention provides a food composition for improving hypertension comprising germinated soybean peptides as an active ingredient.
The term "food composition" as used herein refers to a food prepared by adding the germinated soybean peptide to food materials such as beverage, tea, spices, gum, confectionery, etc., encapsulation, powdering, suspension, etc., But it has the advantage that there are no side effects that can occur when a drug is used for a long period of time using a food as a raw material.
There is no particular limitation on the kind of the food. Examples of the food to which the above substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, and includes foods in a conventional sense.
Since the food composition according to the present invention thus obtained can be taken on a daily basis, a high antihypertensive effect can be expected, which is very useful.
When the germinated soybean peptide is used as a food additive, the germinated soybean peptide may be directly added or used together with other food or other food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed may be within a range that does not impair the original taste of the food, and is usually in the range of 0.01 to 50 parts by weight, preferably 0.1 to 20 parts by weight, based on 100 parts by weight of the whole composition. In the case of foods in the form of granules, tablets or capsules, they may be added in an amount of usually 0.1 to 100 parts by weight, preferably 0.5 to 80 parts by weight. However, in the case of long-term intake intended for health and hygiene purposes or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.
In addition, the food composition according to the present invention may further contain various flavors or natural carbohydrates. The above-mentioned natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau Martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. In addition to the above, the food composition according to the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, Alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the food composition of the present invention may contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. Although the ratio of such additives is not critical, it is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the food composition of the present invention.
According to another embodiment of the present invention, the present invention provides a pharmaceutical composition for improving hypertension comprising germinated soybean peptide as an active ingredient.
The sprout peptides according to the present invention may be in the form of pharmaceutically acceptable salts.
The salt may be an acid addition salt formed using an organic acid or an inorganic acid. Examples of the organic acid include formic acid, acetic acid, propionic acid, lactic acid, butyric acid, isobutyric acid, trifluoroacetic acid, malic acid, maleic acid, malonic acid, But are not limited to, lactic acid, succinic acid, succinic monoamide, glutamic acid, tartaric acid, oxalic acid, citric acid, glycolic acid, glucuronic acid, ascorbic acid, benzoic acid, phthalic acid, salicylic acid, anthranilic acid, dichloroacetic acid, And inorganic acids include, for example, hydrochloric acid, bromic acid, sulfuric acid, phosphoric acid, nitric acid, carbonic acid and boric acid-based salts. Preferably in the hydrochloride or acetate form, more preferably in the hydrochloride form.
Separately, additionally saltable forms include, but are not limited to, the salts of gabapentin, gabapentin, pregabalin, nicotinate, adipate, hemimarate, cysteine, acetylcysteine, methionine, arginine, lysine, Aspartate and the like.
The pharmaceutical composition according to the present invention may further comprise a pharmaceutically acceptable carrier. The carrier may contain various components such as buffer, injectable sterile water, normal saline or phosphate buffered saline, sucrose, histidine, salt and polysorbate.
The content of the germinating soybean peptides in the composition may be appropriately adjusted according to the symptoms of the disease, the progress of the symptoms, the condition of the patient, the administration method, the form of the preparation, etc. For example, Preferably 0.001 to 1 part by weight, based on the weight of the composition. The content ratio is a value based on the dried amount from which the solvent is removed.
The pharmaceutical composition according to the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical preparation, for example, various forms of oral and parenteral administration at the time of clinical administration. In the case of formulation, , Extenders, binders, wetting agents, disintegrating agents, and surfactants.
Solid form preparations for oral administration include tablets, pills, powders, granules, capsules and the like. Such solid preparations can be prepared by adding to the pharmaceutical composition of the present invention at least one or more excipients such as starch, calcium carbonate, Sucrose, lactose, gelatin and the like can be prepared.
In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of liquid formulations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are commonly used simple diluents.
Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.
Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.
The germinated soybean peptides according to the present invention exhibit excellent ACE inhibitory activity and are effective not only for prevention, improvement or treatment of hypertension but also for human safety as a natural material and thus have a very high utilization value.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a diagram showing a process for producing an unrooted soybean peptide. FIG.
2 is a chart showing ACE inhibitory activity of germinated soybean peptides.
Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described in detail below. The present invention may, however, be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Is provided to fully convey the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims.
[Production Example 1]
Process for the production of germinated peptides or germinated peptides
The process of manufacturing germinated peptides adds to the germination process of the beans. The above germination was carried out in a germ capable of intermittently spraying at an interval of about 1 hour after immersing in water at 25 캜 for 4 hours at a temperature of 25 캜 and a light-shielded environment at 25 캜. The germination was germinated until the bud of the bean grew to 1 cm or more, and it took about 2-3 days.
The germinated soybean peptides and germinated soybean peptides are prepared as follows. The germinated soybeans and germinated soybeans were immersed in water for 4 hours, respectively, and then they were removed and melted. Thereafter, 10 times as much water as the weight of soybeans removed and soaked was added and heated to 100 캜 for 15 minutes. Thereafter, the mixture was pulverized at 7,000 rpm for 3 minutes using Turrex, and further heated to 100 ° C for 15 minutes to obtain undiluted soybean extract (SM) and germinated soybean extract (GSM).
In the step of obtaining peptides from the germinated soybean extract, the germinated soybean extracts were treated with proteolytic enzymes (BSM and BGSM, respectively) after centrifugation, and the germinated (germinated) soybean extract (BWSM, BWGSM, respectively) were separated by proteolytic enzymes. The proteolytic enzyme is hydrolyzed by treatment with 0.2% (w / v) of proteolytic enzyme for 2, 4 and 6 hours. The centrifugation is carried out by centrifuging at 10,000 rpm for 15 minutes and then taking the supernatant. The supernatant was obtained by centrifuging the above germinated soybean extracts (SM, GSM, respectively) after proteolytic enzyme treatment, and the germinated soybean extract (SM, GSM, respectively) The enzyme was treated for 10 minutes at 100 ° C to inactivate the proteolytic enzyme. After centrifugation at 10,000 rpm for 15 minutes, the supernatant was taken out and dried in hot air at 70 ° C. and pulverized to prepare germinated soybean peptides (BSM and BGSM, respectively) and germinated soybean peptides (BWSM and BWGSM, respectively).
[Example 1]
ACE inhibitory activity of germinated soybean peptides was measured according to the method of Cushman et al. (See: Cheung, H. S. and Cushman, D. W., Biochem. Pharmacol., 20: 1637 (1971)).
1 g of Lung acetone powder from rabbit (L-0756, Sigma-Aldrich Chemical Co., MO, USA) was extracted with 10 ml of 0.1 M sodium borate buffer (pH 8.3) containing 0.3 M NaCl Respectively. After centrifugation at 12,000 rpm for 30 min, the supernatant was taken and stored at 4 ° C before use.
100 μl of
<Conversion formula 1>
Inhibitory effect (%) = [(E D - E S ) / (E D - E B )] × 100
E D : absorbance of the experimental group treated with distilled water instead of the sample
E S : absorbance of the experimental group treated with the sample
E B : absorbance of the experimental group treated with the sample after stopping the reaction
As a result, as shown in FIG. 2, when the ACE inhibitory activity of the control (Control) was compared with 100%, the positive control group of the caffeopril was 65%, the germinated soybean extract (SM) (BGSM) were found to be 39%, 35%, 48%, and protein degradation by 34% and 34%, respectively, while the germination peptides (BSM) treated with proteolytic enzymes after centrifugation were 19%, 21% and 36% The activity of the germinated soybean peptides (BWSM) after centrifugation was 55%, 60% and 74%, respectively, for 47%, 53% and 66% of germinated soybean peptides (BWGSM) respectively.
From the above results, it can be confirmed that the germinated group has better ACE inhibitory ability than the untreated group, and that when treated with protease after centrifugation, ACE inhibition is more excellent (BSM, BGSM). In addition, ACE inhibition was improved with increasing protease treatment time, and proteolytic enzyme treatment for 6 hours showed ACE inhibition similar to that of positive control group.
The increase in ACE inhibition means that the peptide content is increased as the protease treatment time is increased. This means that the ACE inhibitory effect of germinated soybean peptides is better than that of the normal germination soybean extract .
Claims (6)
Wherein the germinated soybean peptides are contained in an amount of 0.01 to 50 parts by weight based on 100 parts by weight of the whole food composition.
Wherein the hypertension is characterized by an angiotensin converting enzyme inhibitory activity.
Wherein the germinated soybean peptide is contained in an amount of 0.0001 to 10 parts by weight based on 100 parts by weight of the total pharmaceutical composition.
Wherein the hypertension is characterized by an angiotensin converting enzyme inhibitory activity.
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