KR20140114662A - Benzo[a]pyrene responsive genes in Hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same - Google Patents

Abstract

The present invention relates to genes from Hydra magnipapillata, responding to the exposure of benzo[a]pyrene (BaP) and a method for diagnosing an aquatic environment pollution using the same. Specifically, the genes, of which expression is increased or decreased according to the exposure hours of BaP, is detected among genes derived from Hydra magnipapillata. The gene from Hydra magnipapillata responding to BaP is used as a biomarker and is used in a microarray, a diagnosis method, and a kit containing the microarray for detecting or diagnosing aquatic ecosystem stress.

Description

The present invention relates to a method for diagnosing environmental pollution of a water ecosystem using a hydra gene corresponding to benzoprene exposure,

The present invention relates to the use of hydra ( Hydra ) corresponding to exposure to benzo [a] pyrene (BaP) magnipalillata ) gene and a method for diagnosing environmental pollution in aquatic ecosystem using the same.

Polycyclic aromatic hydrocarbons (PAHs) are produced by combustion of fossil fuels or organic-rich materials. Industrial wastes, incineration products, automobile exhaust gases, etc. are important sources of influx. Many polycyclic aromatic hydrocarbons are industrialized Is being detected globally in freshwater and marine sediments in remote areas. Polycyclic aromatic hydrocarbons are adsorbed on suspended solids, precipitated on the bottom of the water, accumulate in sediments, enter the body of an animal or human, cause carcinogenesis, mutagenesis, and interfere with and disturb the action of hormones. In addition, it may be directly introduced from the crude oil or may flow in the refining process. If it is easily adsorbed on the suspended material and deposited on the sea, it may accumulate in the sediments due to hydrophobicity and persistence. Actual US NOAA NS & T business survey showed that the concentration of PAH in the sediments was very high at 29324ng / g dw at a research site in Hudson / Raritan estuary, 10395ng / g dw at Long Island Sound survey site and 15405ng / g dw at Boston Harbor And in the case of Korea, PAH concentrations were recorded at 1207.9ng / g dw at Gyeonggi Bay, 4025.7, 2607.4ng / g dw at Pohang and 3618.6ng / g dw at Pohang Inchon.

Representative PAHs, benzopyrene, are polycyclic aromatic hydrocarbons, which are produced when organic matter is burned or pyrolyzed. Specific pollutants for human health due to toxicity Classification as carcinogen in carcinogenicity classification (IARC) was selected as hazardous chemical in this study. Hydrocarbons, amino acids, starch or fatty acids are produced when heated above 600 ° C. It forms a solid form of yellow crystals at room temperature. It is present in coal tar and contains trace amounts of exhaust gas, cigarette smoke, incineration gas, and smoked food in automobiles.

Hydra magnipalillata ) is the first multicellular animal to have nerve cells, and it is also very important in the study of biological evolution. Breeding as a double-blind animal is by silent and oily reproduction, and has excellent reproduction ability. Biol. 296: 62-70; Kaesbauer et al., 2007), which is involved in morphogenesis (Hobmayer et al., 2000, Nature 407: 186-189; Arvizu et al., 2006, Differentiation 74: 305-312; , Bev. Biol. 303: 376-390), signaling and signal transduction systems associated with regeneration (Bode, 2003, Dev Dyn 226: 225-236; Fujisawa, 2003, Dev Dyn 226: 182-189; , 2003, Dev Dyn 226: 257-267), a study related to signaling of cell differentiation (Thomsen et al., 2004, Mech. Dev. 121: 195-204, Philipp et al., 2005, Gene Expr. 397-402) and tissue fate determining signals (Bielen et al., 2007, Development, 134: 4187-4197). Since these signaling systems are common to all animals through evolution, they can extrapolate signal transduction pathways in the hydra to other animals. In Hydra, many genes that are expressed only in the early developmental stage in most animals are maintained in the adult. Therefore, it is possible to study signal transduction systems related to this without any time limit. Since molecular markers for each cell type are known, studies on cell differentiation are also possible. In situ hybridization (ISH) and immunohistochemistry (IHC) methods have been established for localization of gene and protein expression. Currently, 160,000 clones of ESTs and genome data (Chapman et al., 2010, Nature 464: 592-596) have been DB, and information about genes belonging to various signal transduction systems can be retrieved.

Therefore, the present inventors discovered that, in extracting biomarkers corresponding to benzoprene exposure, gene information from DDBJ / EMBL / NCBI gene database was stored at 17,000 We designed a oligo-probe in a single singleton and completed a 17K Hydra Express Gene Microarray (HEGEM) with them, and used it to identify genes whose expression levels were changed by exposure to benzopyrene The present invention has been accomplished on the basis of the findings that biomarkers and biosensors capable of confirming the exposure of benzopyrene can be effectively used.

The object of the present invention is to provide a hydra ( Hydra ) corresponding to benzo [a] pyrene (BaP) magnipapillata ), a method for confirming exposure to benzopyran using the same, or a method for diagnosing environmental pollution in aquatic ecosystem.

In order to achieve the above object, the present invention provides an oligonucleotide comprising all or a part of the following gene sequence whose expression increases or decreases in response to a benzopyrene change, or an oligonucleotide complementary to the oligonucleotide, A microarray for verifying exposure to benzopyran is provided:

(Heat shock protein 90, alpha), the gene described in SEQ ID NO: 3 (Heat shock protein 70), the gene described in SEQ ID NO: 4 (SEQ ID NO: (Small heat shock protein), a gene described in SEQ ID NO: 6 (Calpain-5), a gene described in SEQ ID NO: 7 (MAtrix metalloproteinase), and SEQ ID NO: 8 (Caspase 7), the gene described in SEQ ID NO: 10 (Eukaryotic translation initiation factor 4 gamma, 3), the gene described in SEQ ID NO: 11 (Tetraspanin 33), the sequence (NADH dehydrogenase 1 alpha subcomplex 4), the gene described in SEQ ID NO: 13 (Complementation group C), the gene described in SEQ ID NO: 14 (Cathepsin L-like protease) Genetically listed (Ikappa B kinase gamma), a vascular endothelial growth factor, a gene described in SEQ ID NO: 17 (Noggin 1), a gene described in SEQ ID NO: 18 (Oxidative stress protein) A gene described in SEQ ID NO: 20 (Sox17 alpha), a gene described in SEQ ID NO: 21 (SRY-box containing gene 32), a gene described in SEQ ID NO: 22 (Homeobox protein Otx) (Calmodulin), a gene described in SEQ ID NO: 24 (Potassium channel homolog), a gene described in SEQ ID NO: 25 (Hydra Na channel 3), a gene described in SEQ ID NO: 26 (Caspase 3 preproprotein), a gene described in SEQ ID NO: 28 (Mediator of DNA damage checkpoint 1), a gene described in SEQ ID NO: 29 (stress-induced-phosphoprotein 1 (Hsp70 / Hsp90-organizing p (Neogenin), a gene (Pellino homolog 1) described in SEQ ID NO: 31, a gene described in SEQ ID NO: 32 (complement component C3-like protein), a gene described in SEQ ID NO: A gene described in SEQ ID NO: 34 (Forkhead box-containing transcription factor FoxQ2b), a gene described in SEQ ID NO: 35 (Homeodomain protein Otx), SEQ ID NO: 36 (Hym-176 preprohormone), the gene described in SEQ ID NO: 37 (Hym-355 preprohormone), the gene described in SEQ ID NO: 38 (Calcium transporter 2), the gene described in SEQ ID NO: 39 (Calmodulin CG8472- A gene described in SEQ ID NO: 41 (Hydra Na channel 4 (hynac4)), a gene described in SEQ ID NO: 42 (Hydra Na channel 2), a gene described in SEQ ID NO: SEQ ID NO: 43 (Calcium channel, voltage-dependent, alpha2 / delta subunit 1), a gene described in SEQ ID NO: 44 (Goosecoid), a gene described in SEQ ID NO: 45 (Cholinergic receptor, nicotinic, alpha 9) (TNF-receptor-associated factor 1), a gene described in SEQ ID NO: 49 (Rho (SEQ ID NO: 49)), a gene described in SEQ ID NO: GTPase activating protein 6), a gene described in SEQ ID NO: 50 (Neuronal acetylcholine receptor subunit alpha-7), a gene described in SEQ ID NO: 51 (Periculin), a gene described in SEQ ID NO: 52 (Senataxin) A gene described in SEQ ID NO: 54 (DnaJ subfamily A member 2), a gene described in SEQ ID NO: 55 (Ubiquitin specific peptidase 8), a gene described in SEQ ID NO: 56 (Cd36 / Scavenger (FAT tumor suppressor homolog 4), the gene (Notch) described in SEQ ID NO: 58, the gene described in SEQ ID NO: 59 (Novel EGF domain containing protein 61, a gene described in SEQ ID NO: 61 (Aquaporin 9a), a gene described in SEQ ID NO: 62 (Translationally controlled tumor protein), a gene described in SEQ ID NO: 63 (Fibroblast growth factor 24, a gene described in SEQ ID NO: 64 (Ferritin), a gene described in SEQ ID NO: 65 (Ropporin 1 -like), and a gene described in SEQ ID NO: 66 (EGF-like module containing mucin- .

In addition, the present invention provides a method for confirming exposure to benzopyran using the genes according to the present invention.

The present invention also provides a kit for confirming exposure to benzopyran, which comprises the microarray according to the present invention.

In addition, the present invention provides a kit for confirming exposure to benzopyran, which comprises a pair of primers complementary to each gene and capable of gene amplification for the genes according to the present invention.

The Hydra magnipapillata derived gene corresponding to the benzoprene (Benzo [a] pyrene, BaP) exposure of the present invention and the method for diagnosing aquatic ecosystem using the hydra magnipapillata gene according to the present invention measure the change in expression amount of a specific hydra- , It can be usefully used as a biosensor and a kit for confirming whether benzoprene is exposed or not.

1 is benzo differential gene expression profiling of Hydra exposed to pyrene Hierachial clustering result with 48-h LC 4 hours in 500 μg / L (ppb), which corresponds to approximately 1/100 of the _50, 12, 24 and 48 hours of exposure.

Hereinafter, the present invention will be described in detail.

The present invention relates to a hydra ( Hydra ) which changes in expression in response to benzopyrene (BaP) magnipapillata) was identify genes derived from those, we identify the Hydra-derived gene that changes the amount of differentially expressed according to the exposure time of the benzo pyrene. Therefore, a microarray in which hydra-derived genes whose expression levels vary with respect to the benzopyrene are integrated can be used to detect the environmental pollution of aquatic ecosystem and to diagnose its condition.

The present invention provides a microarray for confirming the expression of benzopyran, in which an oligonucleotide of a nucleic acid sequence of any one or more genes selected from the following group or its complementary strand molecule is directly introduced:

(Heat shock protein 90, alpha), the gene described in SEQ ID NO: 3 (Heat shock protein 70), the gene described in SEQ ID NO: 4 (SEQ ID NO: (Small heat shock protein), a gene described in SEQ ID NO: 6 (Calpain-5), a gene described in SEQ ID NO: 7 (MAtrix metalloproteinase), and SEQ ID NO: 8 (Caspase 7), the gene described in SEQ ID NO: 10 (Eukaryotic translation initiation factor 4 gamma, 3), the gene described in SEQ ID NO: 11 (Tetraspanin 33), the sequence (NADH dehydrogenase 1 alpha subcomplex 4), the gene described in SEQ ID NO: 13 (Complementation group C), the gene described in SEQ ID NO: 14 (Cathepsin L-like protease) Genetically listed (Ikappa B kinase gamma), a vascular endothelial growth factor, a gene described in SEQ ID NO: 17 (Noggin 1), a gene described in SEQ ID NO: 18 (Oxidative stress protein) A gene described in SEQ ID NO: 20 (Sox17 alpha), a gene described in SEQ ID NO: 21 (SRY-box containing gene 32), a gene described in SEQ ID NO: 22 (Homeobox protein Otx) (Calmodulin), a gene described in SEQ ID NO: 24 (Potassium channel homolog), a gene described in SEQ ID NO: 25 (Hydra Na channel 3), a gene described in SEQ ID NO: 26 (Caspase 3 preproprotein), a gene described in SEQ ID NO: 28 (Mediator of DNA damage checkpoint 1), a gene described in SEQ ID NO: 29 (stress-induced-phosphoprotein 1 (Hsp70 / Hsp90-organizing p (Neogenin), a gene (Pellino homolog 1) described in SEQ ID NO: 31, a gene described in SEQ ID NO: 32 (complement component C3-like protein), a gene described in SEQ ID NO: A gene described in SEQ ID NO: 34 (Forkhead box-containing transcription factor FoxQ2b), a gene described in SEQ ID NO: 35 (Homeodomain protein Otx), SEQ ID NO: 36 (Hym-176 preprohormone), the gene described in SEQ ID NO: 37 (Hym-355 preprohormone), the gene described in SEQ ID NO: 38 (Calcium transporter 2), the gene described in SEQ ID NO: 39 (Calmodulin CG8472- A gene described in SEQ ID NO: 41 (Hydra Na channel 4 (hynac4)), a gene described in SEQ ID NO: 42 (Hydra Na channel 2), a gene described in SEQ ID NO: SEQ ID NO: 43 (Calcium channel, voltage-dependent, alpha2 / delta subunit 1), a gene described in SEQ ID NO: 44 (Goosecoid), a gene described in SEQ ID NO: 45 (Cholinergic receptor, nicotinic, alpha 9) (TNF-receptor-associated factor 1), a gene described in SEQ ID NO: 49 (Rho (SEQ ID NO: 49)), a gene described in SEQ ID NO: GTPase activating protein 6), a gene described in SEQ ID NO: 50 (Neuronal acetylcholine receptor subunit alpha-7), a gene described in SEQ ID NO: 51 (Periculin), a gene described in SEQ ID NO: 52 (Senataxin) A gene described in SEQ ID NO: 54 (DnaJ subfamily A member 2), a gene described in SEQ ID NO: 55 (Ubiquitin specific peptidase 8), a gene described in SEQ ID NO: 56 (Cd36 / Scavenger (FAT tumor suppressor homolog 4), the gene (Notch) described in SEQ ID NO: 58, the gene described in SEQ ID NO: 59 (Novel EGF domain containing protein 61, a gene described in SEQ ID NO: 61 (Aquaporin 9a), a gene described in SEQ ID NO: 62 (Translationally controlled tumor protein), a gene described in SEQ ID NO: 63 (Fibroblast growth factor 24, a gene described in SEQ ID NO: 64 (Ferritin), a gene described in SEQ ID NO: 65 (Ropporin 1 -like), and a gene described in SEQ ID NO: 66 (EGF-like module containing mucin- .

The gene is called Hydra magnapapillata ), but is not limited thereto.

(Heat shock protein 90, alpha), the gene described in SEQ ID NO: 3 (Heat shock protein 70), the gene described in SEQ ID NO: 4 5, a gene described in SEQ ID NO: 7 (MAtrix metalloproteinase), a gene described in SEQ ID NO: 8, a gene described in SEQ ID NO: (Caspase 7), the gene described in SEQ ID NO: 10 (Eukaryotic translation initiation factor 4 gamma, 3) and the gene described in SEQ ID NO: 11 (Tetraspanin 33) But is not limited to, a gene whose expression level is increased or decreased by a 4-hour exposure to benzopyran compared to a normal control group.

(Heat shock protein 90, alpha), a gene described in SEQ ID NO: 3 (Heat shock protein 70), a gene described in SEQ ID NO: 4 (Glutathione S-transferase), SEQ ID NO: 5 (Caspase 7), the gene described in SEQ ID NO: 12 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (small heat shock protein), the gene described in SEQ ID NO: (Complementation group C), a gene described in SEQ ID NO: 14 (Cathepsin L-like protease), a gene described in SEQ ID NO: 15 (IkappaB kinase gamma), a gene described in SEQ ID NO: 16 (Noggin 1), a gene described in SEQ ID NO: 18 (Oxidative stress protein), a gene described in SEQ ID NO: 19 (SNF1-like kinase 2), a gene represented by SEQ ID NO: Described as A gene described in SEQ ID NO: 22 (Homeobox protein Otx), a gene described in SEQ ID NO: 23 (Calmodulin), a gene represented by SEQ ID NO: 24 The gene (Potassium channel homolog) described and the gene described in SEQ ID NO: 25 (Hydra Na channel 3) are preferably genes whose expression level is increased or decreased by 12 hours exposure to benzopyran compared to the normal control group, Do not.

(Heat shock protein 90, alpha), a gene described in SEQ ID NO: 3 (Heat shock protein 70), a gene described in SEQ ID NO: 4 (Glutathione S-transferase), SEQ ID NO: 5 (Caspase 7), the gene described in SEQ ID NO: 12 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4), the gene described in SEQ ID NO: 15 (IkappaB kinase (SNF1-like kinase 2), a gene described in SEQ ID NO: 20 (Sox17 alpha), a gene described in SEQ ID NO: 21 (SRY-box containing gene 32), SEQ ID NO: 24 (Vasa-related protein CnVAS1), a gene described in SEQ ID NO: 27 (Caspase 3 preproprotein), a gene described in SEQ ID NO: 28 (Mediator of DNA damage checkpoint 1), SEQ ID NO: 29 30, a gene described in SEQ ID NO: 30 (Neogenin), a gene described in SEQ ID NO: 31 (Pellino homolog 1), SEQ ID NO: 32 (DnaJ (Hsp40) homolog, subfamily C, member 3), the gene described in SEQ ID NO: 34 (Forkhead box-containing transcription factor FoxQ2b), the sequence (Hym-176 preprohormone), the gene described in SEQ ID NO: 37 (Hym-355 preprohormone), the gene described in SEQ ID NO: 38 (Calcium transporter (Calmodulin CG8472-PA), the gene described in SEQ ID NO: 40 (Potassium voltage-gated channel subfamily A member 1), the gene described in SEQ ID NO: 41 (Hydra Na channel 4 )), SEQ ID NO: 42 (Calcium channel, voltage-dependent, alpha2 / delta subunit 1), the gene described in SEQ ID NO: 44 (Goosecoid), the gene described in SEQ ID NO: 45 (Cholinergic receptor, nicotinic, alpha 9), a gene described in SEQ ID NO: 46, an epidermal growth factor receptor, a gene described in SEQ ID NO: 48 (TNF- receptor-associated factor 1, Rho GTPase activating protein 6, Neuronal acetylcholine receptor subunit alpha-7, and Periculin, SEQ ID NO: 51, But is not limited to, a gene whose expression level is increased or decreased by 24 hours exposure to benzopyran compared to a normal control group.

(Heat shock protein 90, alpha), the gene described in SEQ ID NO: 3 (Heat shock protein 70), the gene described in SEQ ID NO: 4 (Caspase 7), the gene described in SEQ ID NO: 12 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4), the gene described in SEQ ID NO: 15 (Ikappa B kinase gamma) A gene described in SEQ ID NO: 20 (Sox17 alpha), a gene described in SEQ ID NO: 23 (Calmodulin), a gene described in SEQ ID NO: 24 (Potassium channel homolog) A gene described in SEQ ID NO: 30, a gene described in SEQ ID NO: 36 (Hym-176 preprohormone), a gene described in SEQ ID NO: 37 (Hym-related protein CnVAS1) -355 preprohormone), a gene described in SEQ ID NO: 43 (calcium channel, voltage-dependent, alpha2 / delta subunit 1), a gene described in SEQ ID NO: 45 (Cholinergic receptor, nicotinic, alpha 9) A gene described in SEQ ID NO: 51, a gene described in SEQ ID NO: 52 (Senataxin), a gene represented by SEQ ID NO: A gene described in SEQ ID NO: 54 (DnaJ subfamily A member 2), a gene described in SEQ ID NO: 55 (Ubiquitin specific peptidase 8), a gene described in SEQ ID NO: 56 (Cd36 / (FAT tumor suppressor homolog 4), a gene described in SEQ ID NO: 58 (Notch), a gene described in SEQ ID NO: 59 (Novel EGF domain protein), A gene described in SEQ ID NO: 60 (Aquaporin 9a), a gene described in SEQ ID NO: 62 (Translationally controlled tumor protein), a gene described in SEQ ID NO: 63 (Fibroblast growth factor 24, the gene described in SEQ ID NO: 64 (Ferritin), the gene described in SEQ ID NO: 65 (Ropporin 1-like) and the gene described in SEQ ID NO: 66 (EGF-like module containing mucin-like sequence 4) But it is preferably, but not limited to, a gene whose expression level is increased or decreased by 48 hours exposure to pyrene compared to the normal control group.

In addition,

1) separating the RNA from the experimental group hydra and the control group, hydra, respectively;

2) labeling the experimental group and the control group with different fluorescent substances while synthesizing cDNA from the RNA of the experimental group and the control group of step 1);

3) hybridizing the cDNA labeled with the different fluorescent material of step 2) with the microarray of claim 1;

4) analyzing the reacted microarray; And

And 5) comparing the gene expression level accumulated in the microarray of claim 1 with that of the control in the analyzed data to provide a method for confirming exposure to benzopyran.

The fluorescent material of step 2) is preferably selected from the group consisting of Cy3, Cy5, poly-L-lysine-fluorescein isothiocyanate (RITC), rhodamine-B-isothiocyanate (RITC), and rhodamine. I never do that.

In addition,

1) separating the RNA from the hydra of the test group exposed to the test sample and the hydra of the normal control group, respectively;

2) Performing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) using primer pair complementary to each of the following genes and amplifying the gene, step; And

(Heat shock protein 90, alpha), the gene described in SEQ ID NO: 3 (Heat shock protein 70), the gene described in SEQ ID NO: 4 (SEQ ID NO: (Small heat shock protein), a gene described in SEQ ID NO: 6 (Calpain-5), a gene described in SEQ ID NO: 7 (MAtrix metalloproteinase), and SEQ ID NO: 8 (Caspase 7), the gene described in SEQ ID NO: 10 (Eukaryotic translation initiation factor 4 gamma, 3), the gene described in SEQ ID NO: 11 (Tetraspanin 33), the sequence (NADH dehydrogenase 1 alpha subcomplex 4), the gene described in SEQ ID NO: 13 (Complementation group C), the gene described in SEQ ID NO: 14 (Cathepsin L-like protease) Genetically listed (Ikappa B kinase gamma), a vascular endothelial growth factor, a gene described in SEQ ID NO: 17 (Noggin 1), a gene described in SEQ ID NO: 18 (Oxidative stress protein) A gene described in SEQ ID NO: 20 (Sox17 alpha), a gene described in SEQ ID NO: 21 (SRY-box containing gene 32), a gene described in SEQ ID NO: 22 (Homeobox protein Otx) (Calmodulin), a gene described in SEQ ID NO: 24 (Potassium channel homolog), a gene described in SEQ ID NO: 25 (Hydra Na channel 3), a gene described in SEQ ID NO: 26 (Caspase 3 preproprotein), a gene described in SEQ ID NO: 28 (Mediator of DNA damage checkpoint 1), a gene described in SEQ ID NO: 29 (stress-induced-phosphoprotein 1 (Hsp70 / Hsp90-organizing p (Neogenin), a gene (Pellino homolog 1) described in SEQ ID NO: 31, a gene described in SEQ ID NO: 32 (complement component C3-like protein), a gene described in SEQ ID NO: A gene described in SEQ ID NO: 34 (Forkhead box-containing transcription factor FoxQ2b), a gene described in SEQ ID NO: 35 (Homeodomain protein Otx), SEQ ID NO: 36 (Hym-176 preprohormone), the gene described in SEQ ID NO: 37 (Hym-355 preprohormone), the gene described in SEQ ID NO: 38 (Calcium transporter 2), the gene described in SEQ ID NO: 39 (Calmodulin CG8472- A gene described in SEQ ID NO: 41 (Hydra Na channel 4 (hynac4)), a gene described in SEQ ID NO: 42 (Hydra Na channel 2), a gene described in SEQ ID NO: SEQ ID NO: 43 (Calcium channel, voltage-dependent, alpha2 / delta subunit 1), a gene described in SEQ ID NO: 44 (Goosecoid), a gene described in SEQ ID NO: 45 (Cholinergic receptor, nicotinic, alpha 9) (TNF-receptor-associated factor 1), a gene described in SEQ ID NO: 49 (Rho (SEQ ID NO: 49)), a gene described in SEQ ID NO: GTPase activating protein 6), a gene described in SEQ ID NO: 50 (Neuronal acetylcholine receptor subunit alpha-7), a gene described in SEQ ID NO: 51 (Periculin), a gene described in SEQ ID NO: 52 (Senataxin) A gene described in SEQ ID NO: 54 (DnaJ subfamily A member 2), a gene described in SEQ ID NO: 55 (Ubiquitin specific peptidase 8), a gene described in SEQ ID NO: 56 (Cd36 / Scavenger (FAT tumor suppressor homolog 4), the gene (Notch) described in SEQ ID NO: 58, the gene described in SEQ ID NO: 59 (Novel EGF domain containing protein 61, a gene described in SEQ ID NO: 61 (Aquaporin 9a), a gene described in SEQ ID NO: 62 (Translationally controlled tumor protein), a gene described in SEQ ID NO: 63 (Fibroblast growth factor 24, a gene described in SEQ ID NO: 64 (Ferritin), a gene described in SEQ ID NO: 65 (Ropporin 1 -like), and a gene described in SEQ ID NO: 66 (EGF-like module containing mucin-

3) comparing the gene product of step 2) with a control group to confirm the degree of expression.

In addition, the present invention provides a kit for confirming the exposure of benzo [a] pyrene (BaP) containing the microarray of the present invention.

The kit may further include any one selected from the group consisting of a fluorescent substance group consisting of a streptavidin-like phosphatease conjugate, a chemiluminescent substance, and a chemiluminescent substance However, the present invention is not limited thereto.

The kit may be any one selected from the group consisting of buffer solutions used for hybridization, reverse transcriptase for the synthesis of cDNA from RNA, reaction reagents consisting of dNTPs and rNTPs (premixed or separated feeds), labeled reagents, and wash buffer solutions But are not limited thereto. Any reaction reagent necessary for a hybridization reaction known to a person skilled in the art can be included.

In addition, the present invention provides a kit for confirming exposure to Benzo [a] pyrene (BaP) comprising a pair of primers complementary to each of the following genes and capable of amplifying a gene:

(Heat shock protein 90, alpha), the gene described in SEQ ID NO: 3 (Heat shock protein 70), the gene described in SEQ ID NO: 4 (SEQ ID NO: (Small heat shock protein), a gene described in SEQ ID NO: 6 (Calpain-5), a gene described in SEQ ID NO: 7 (MAtrix metalloproteinase), and SEQ ID NO: 8 (Caspase 7), the gene described in SEQ ID NO: 10 (Eukaryotic translation initiation factor 4 gamma, 3), the gene described in SEQ ID NO: 11 (Tetraspanin 33), the sequence (NADH dehydrogenase 1 alpha subcomplex 4), the gene described in SEQ ID NO: 13 (Complementation group C), the gene described in SEQ ID NO: 14 (Cathepsin L-like protease) Genetically listed (Ikappa B kinase gamma), a vascular endothelial growth factor, a gene described in SEQ ID NO: 17 (Noggin 1), a gene described in SEQ ID NO: 18 (Oxidative stress protein) A gene described in SEQ ID NO: 20 (Sox17 alpha), a gene described in SEQ ID NO: 21 (SRY-box containing gene 32), a gene described in SEQ ID NO: 22 (Homeobox protein Otx) (Calmodulin), a gene described in SEQ ID NO: 24 (Potassium channel homolog), a gene described in SEQ ID NO: 25 (Hydra Na channel 3), a gene described in SEQ ID NO: 26 (Caspase 3 preproprotein), a gene described in SEQ ID NO: 28 (Mediator of DNA damage checkpoint 1), a gene described in SEQ ID NO: 29 (stress-induced-phosphoprotein 1 (Hsp70 / Hsp90-organizing pr (Neogenin), the gene (Pellino homolog 1) described in SEQ ID NO: 31, the complementary component C3-like protein (SEQ ID NO: 32) A gene described in SEQ ID NO: 34 (Forkhead box-containing transcription factor FoxQ2b), a gene described in SEQ ID NO: 35 (Homeodomain protein Otx), SEQ ID NO: 36 (Hym-176 preprohormone), the gene described in SEQ ID NO: 37 (Hym-355 preprohormone), the gene described in SEQ ID NO: 38 (Calcium transporter 2), the gene described in SEQ ID NO: 39 (Calmodulin CG8472- A gene described in SEQ ID NO: 41 (Hydra Na channel 4 (hynac4)), a gene described in SEQ ID NO: 42 (Hydra Na channel 2), a gene described in SEQ ID NO: SEQ ID NO: 43 (Calcium channel, voltage-dependent, alpha2 / delta subunit 1), a gene described in SEQ ID NO: 44 (Goosecoid), a gene described in SEQ ID NO: 45 (Cholinergic receptor, nicotinic, alpha 9) (TNF-receptor-associated factor 1), a gene described in SEQ ID NO: 49 (Rho (SEQ ID NO: 49)), a gene described in SEQ ID NO: GTPase activating protein 6), a gene described in SEQ ID NO: 50 (Neuronal acetylcholine receptor subunit alpha-7), a gene described in SEQ ID NO: 51 (Periculin), a gene described in SEQ ID NO: 52 (Senataxin) A gene described in SEQ ID NO: 54 (DnaJ subfamily A member 2), a gene described in SEQ ID NO: 55 (Ubiquitin specific peptidase 8), a gene described in SEQ ID NO: 56 (Cd36 / Scavenger rec (FAT tumor suppressor homolog 4), the gene (Notch) described in SEQ ID NO: 58, the gene described in SEQ ID NO: 59 (Novel EGF domain containing protein 61, a gene described in SEQ ID NO: 61 (Aquaporin 9a), a gene described in SEQ ID NO: 62 (Translationally controlled tumor protein), a gene described in SEQ ID NO: 63 (Fibroblast growth factor 24, a gene described in SEQ ID NO: 64 (Ferritin), a gene described in SEQ ID NO: 65 (Ropporin 1 -like), and a gene described in SEQ ID NO: 66 (EGF-like module containing mucin- .

In a specific example of the present invention, hydra is exposed to benzopyrene at various times to discover genes derived from Hydra magnipapillata corresponding to the benzopyrene change, and hydra-derived cDNA is synthesized, Respectively. Specifically, mRNA was isolated from hydra which was exposed to benzopyrene for 4 hours, 12 hours, 24 hours and 48 hours, respectively, and then synthesized by cDNA. The synthesized cDNA was fluorescently labeled with Cy3-CTP and Cy5-CTP, and a microarray was constructed using the fluorescently labeled Cy3-CTP and Cy5-CTP. The prepared microarrays were scanned using an Axon GenePix 4000B scanner (Axon Instrument, USA) and analyzed. As a result, the expression levels were increased in experimental groups exposed to benzopyran for 12 hours, nine major genes whose expression levels were increased in the experimental group exposed to benzopyran for 4 hours, two major genes (see Table 2) 14 major genes, 6 decreasing major genes (see Table 3), 16 major genes with increased expression levels in experimental groups exposed to benzopyran for 24 hours, 22 major genes (see Table 4) and 48 20 major genes with increased expression levels and 16 decreasing major genes (see Table 5) were identified in the experimental groups exposed to benzopyrene over time. Therefore, it can be used as a biomarker for confirming the stress and state due to changes in the external environment by confirming the change in the expression level of the hydra-derived gene as a freshwater organism.

Therefore, these genes can be usefully used as microarrays and kits for confirming the exposure of benzopyran.

< Example  1> Culture of hydra and exposure to benzopyrene

<1-1> Culture of hydra

Hydra magnapapillata ) wild strain 105 was deposited in 2002 by the National Institute of Genetics of Japan and has been maintained by the Korea Ocean Research and Development Institute until now. Hydra _{1 mM NaCl, 1 mM CaCl 2} , 0.1 mM KCl, 0.1 mM MgSO 4 And 1 mM Tris (hydroxymethyl) aminoethane (pH 7.6). The water temperature was fixed at 20 ° C, and freshly hatched Artemia larvae were fed to the food every two days. After feeding, the culture medium was exchanged after several hours.

<1-2> Exposure to benzopyran

In order to establish the exposure conditions for benzo [a] pyrene (BaP) in hydra, the lethal concentration 50 (LC ₅₀ ) for benzopyrene was determined and shown in Table 1. The exposure concentration was 48 h LC ₅₀ 20 of hydra were exposed to 500 μg / L, which corresponds to 1/100 of the concentration (56.6 mg / L), for 4 hours, 12 hours, 24 hours and 48 hours, respectively.

Half-lethal concentration of benzopyrene in hydra Exposure time LC ₅₀ (mg / L) 24 hours 78.5 48 hours 56.6 72 hours 28.9

< Example  2> Measurement of gene expression level by exposure to benzopyrene

<2-1> RNAdml  Separation and cDNA  synthesis

Tri-reagent (Molecular Research Center Inc.) was used for RNA extraction of each exposed group. CRNA was synthesized using reverse transcriptase using extracted RNA as a template.

<2-2> Cy3  And Cy5  sign

The total RNAs from the purified exposed and control groups were labeled with Cy3 and Cy5 using Agilent's Low RNA Input Linear Amplification Kit Plus (Agilent Technologies, USA) as follows.

Specifically, 1 μg of the purified hydra RNA was mixed with dT-promoter primer (dT-promoter primer) and MMLV-reverse transcription enzyme (MMLV-Reverse Transcription), followed by reverse transcription at 40 ° C. for 2 hours, T7 polymerase was added and linear amplification was performed at 40 ° C for 2 hours. The samples of the control and experimental groups were labeled with Cy3-CTP and Cy5-CTP, respectively.

<2-3> Hybridization ( Hybridization ) And scanning ( scanning )

Fluorescent labeled cRNA samples were purified using Qiagen PCR purification kit and eluted with distilled water.

Specifically, purified fluorescent-labeled cRNA samples were added to a hybridization buffer (3x SSC, 0.3% SDS, 50% formamide, 20 ug Cot-1 DNA, 20 ug yeast tRNA) And concentrated to form a hybridization mixture. The hybridization mixture was denatured by heating at 95 DEG C for 3 minutes, centrifuged at 12,000 g for 30 seconds, and the temperature was cooled. Then, the prepared hydra microarray was covered with a coverslip, and the modified hybridization mixture was pipetted. The microarray was placed in a GT-Hyb chamber and reacted at 65 ° C for 16 hours. After hybridization was completed, the microarray was removed from the chamber and cleaned in the following order. The microarray was rotated, dried, and stored in a cow until scanning. The completed hydra microarray was scanned using an Axon GenePix 4000B scanner (Axon Instrument, USA). In the GenePix Pro 6.0 program, each spot is scanned from a scanned image using a gridding file and squared to determine the Cy5 / Cy3 intensity of each spot intensity, and ratio (Ratio).

<2-4> Microarray  Data analysis

GeneSpring 7.3.1 (Agilent Technologies, USA) was used to analyze the GPR files obtained from the GenePix Pro program.

Specifically, normalization was performed using LOWESS (locally weighted regression scatterplot smoothing). A reliable gene is defined as a region where the sum of the medians is less than the back ground value or the standard deviation of each pixel value is not significant. We obtained a significant gene by flag-out. Significant genes were selected as spots with a difference of more than two times the value of the normalized ratio.

As a result, 84 genes (48 increased, 36 decreased) related to molecular chaperone, antioxidant enzyme, carcinogenesis, apoptosis and tissue necrosis were exposed in the 4 h exposure group of benzo [a] pyrene, BaP); 113 genes (77 increased, 36 decreased) related to development, inflammation, calcium metabolism, Na-K channel, and innate immunity in 12 hour exposure group of benzopyrene; In the 24 hour exposure group of benzopyran, 242 species (increase of 117 species, decrease of 125 species) related to neuron-related, neurotransmission, innate immunity, tumor formation; The gene expression of 273 species (165 increased, 108 decreased) related to DNA repair, ubiquitination, development / differentiation, tumor and carcinoma was analyzed in the 48 hour exposure group of benzopyrene. Major genes whose expression levels are changed in each exposure group are shown in [Table 2], [Table 3], [Table 4] and [Table 5].

As a result, at the beginning of benzopyran exposure, hydra undergoes oxidative stress, inducing an increase in the expression of molecular chaperone to overcome various stresses including this, and when 12 hours of benzopyrene exposure occurs, inflammation, , Na-K channel, and immune system abnormalities occur. At 24 hours after exposure to benzopyrene, abnormality occurs in the nervous system and immune system, tumor formation is induced, and DNA damage becomes severe after 48 hours of benzopyrene exposure , The expression of genes related to DNA repair is induced and various proteins are damaged, resulting in an increase in the ubiquitination labeled for degradation thereof, abnormalities in development / differentiation, and induction of tumor and carcinogenesis Respectively.

Benzopyran 4 hours In the exposed group  List of key genes of hydra in which the expression level is changed Gene and SEQ ID NO: Change in drainage Cathepsin L (SEQ ID NO: 1) +2.11 Heat shock protein 90, alpha (SEQ ID NO: 2) +4.55  Heat shock protein 70 (SEQ ID NO: 3) +2.13 Glutathione S-transferase (SEQ ID NO: 4) +2.25  Small heat shock protein (SEQ ID NO: 5) +2.14 Calpain-5 (SEQ ID NO: 6) +2.62 MAtrix metalloproteinase (SEQ ID NO: 7) +2.04 Ks1 protein (SEQ ID NO: 8) +2.11 Caspase 7 (SEQ ID NO: 9) +2.26 Eukaryotic translation initiation factor 4 gamma, 3 (SEQ ID NO: 10) -2.33  Tetraspanin 33 (SEQ ID NO: 11) -3.23

Benzopyran 12 hours In the exposed group  List of key genes of hydra in which the expression level is changed Gene and SEQ ID NO: Change in drainage NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (SEQ ID NO: 12) +2.28 Complementation group C (SEQ ID NO: 13) + 2.82 Heatshock protein 90, alpha (SEQ ID NO: 2) +3.88 Cathepsin L-like protease (SEQ ID NO: 14) +2.05 IkappaB kinase gamma (SEQ ID NO: 15) + 2.17 Glutathione S-transferase (SEQ ID NO: 4) +3.85 Small heat shock protein (SEQ ID NO: 5) + 2.84 Heat shock protein 70 (SEQ ID NO: 3) +13.49 Vascular endothelial growth factor (SEQ ID NO: 16) +2.79 Noggin 1 (SEQ ID NO: 17) +2.09 Oxidative stress protein (SEQ ID NO: 18) +6.73 SNF1-like kinase 2 (SEQ ID NO: 19) +2.59 Ks1 protein (SEQ ID NO: 8) +2.18 Caspase 7 (SEQ ID NO: 9) +2.28 Sox17 alpha (SEQ ID NO: 20) -2.56 SRY-box containing gene 32 (SEQ ID NO: 21) -2.22 Homeobox protein Otx (SEQ ID NO: 22) -2.00 Calmodulin (SEQ ID NO: 23) -2.70 Potassium channel homolog (SEQ ID NO: 24) -2.17 Hydra Na channel (SEQ ID NO: 25) -2.22

Benzopyran 24 hours In the exposed group  List of key genes of hydra in which the expression level is changed gene Change in drainage Vasa-related protein CnVAS1 (SEQ ID NO: 26) +2.42 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (SEQ ID NO: 12) +2.92 Caspase 3 preproprotein (SEQ ID NO: 27) + 2.17 Mediator of DNA damage checkpoint 1 (SEQ ID NO: 28) +2.45 Glutathione S-transferase (SEQ ID NO: 4) +4.02 Heat shock protein 70 (SEQ ID NO: 3) +2.37 Heat shock protein 90, alpha (SEQ ID NO: 2) +4.27 IkappaB kinase gamma (SEQ ID NO: 15) +2.29 Small heat shock protein (SEQ ID NO: 5) +2.39 Stress-induced-phosphoprotein 1 (Hsp70 / Hsp90-organizing protein)
(SEQ ID NO: 29) +2.08 SNF1-like kinase 2 (SEQ ID NO: 19) + 2.95 Caspase 7 (SEQ ID NO: 9) +2.30 Neogenin (SEQ ID NO: 30) + 2.17 Pellino homolog 1 (SEQ ID NO: 31) +2.32 Complement component C3-like protein (SEQ ID NO: 32) + 2.00 DnaJ (Hsp40) homolog, subfamily C, member 3 (SEQ ID NO: 33) +2.05 Forkhead box-containing transcription factor FoxQ2b (SEQ ID NO: 34) -2.04 Homeodomain protein Otx (SEQ ID NO: 35) -2.86 Sox17 alpha (SEQ ID NO: 20) -5.56 SRY-box containing gene 32 (SEQ ID NO: 21) -5.26 Hym-176 preprohormone (SEQ ID NO: 36) -3.57 Hym-355 preprohormone (SEQ ID NO: 37) -2.17 Calcium transporter 2 (SEQ ID NO: 38) -2.94 Calmodulin CG8472-PA (SEQ ID NO: 39) -7.14 Potassium channel homolog (SEQ ID NO: 24) -5.00 Potassium voltage-gated channel subfamily A member 1 (SEQ ID NO: 40) -2.38 Hydra Na channel 4 (hynac4) (SEQ ID NO: 41) -2.70 Hydra Na channel 2 (SEQ ID NO: 42) -3.23 Calcium channel, voltage-dependent, alpha2 / delta subunit 1 (SEQ ID NO: 43) -2.86 Goosecoid (SEQ ID NO: 44) -2.33 Cholinergic receptor, nicotinic, alpha 9 (SEQ ID NO: 45) -2.94 Similar to deleted in colorectal carcinoma (SEQ ID NO: 46) -2.63 Epidermal growth factor receptor (SEQ ID NO: 47) -2.38 TNF-receptor-associated factor 1 (SEQ ID NO: 48) -3.13 Rho GTPase activating protein 6 (SEQ ID NO: 49) -2.86 Neuronal acetylcholine receptor subunit alpha-7 (SEQ ID NO: 50) -5.00 Periculin (SEQ ID NO: 51) -2.70

Benzopyran 48 hours In the exposed group  List of key genes of hydra in which the expression level is changed gene Change in drainage Senataxin (SEQ ID NO: 52) +2.27 Vasa-related protein CnVAS1 (SEQ ID NO: 26) +2.34 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (SEQ ID NO: 12) + 5.81 RAD50 homolog (SEQ ID NO: 53) +3.21 Cathepsin L (SEQ ID NO: 1) +2.13 DnaJ subfamily member 2 (SEQ ID NO: 54) +2.34 Glutathione S-transferase (SEQ ID NO: 4) + 5.87 Heat shock protein 70 (SEQ ID NO: 3) +6.62 Heat shock protein 90, alpha (SEQ ID NO: 2) + 4.76 IkappaB kinase gamma (SEQ ID NO: 15) +2.66 Ubiquitin specific peptidase 8 (SEQ ID NO: 55) +2.15 Vascular endothelial growth factor (SEQ ID NO: 16) +3.69 Caspase 7 (SEQ ID NO: 9) +2.46 Cd36 / Scavenger receptor class B, member 1-like protein (SEQ ID NO: 56) +2.21 FAT tumor suppressor homolog 4 (SEQ ID NO: 57) +2.40 Neogenin (SEQ ID NO: 30) +2.46 Notch (SEQ ID NO: 58) + 2.17 Novel EGF domain containing protein (SEQ ID NO: 59) +2.42 TNF-receptor-associated factor 1 (SEQ ID NO: 48) +2.16 Tyrosine kinase receptor (SEQ ID NO: 60) +9.00 Aquaporin 9a (SEQ ID NO: 61) -2.50 Translationally controlled tumor protein (SEQ ID NO: 62) -3.45 Sox17 alpha (SEQ ID NO: 20) -2.13 Fibroblast growth factor 24 (SEQ ID NO: 63) -2.05 Hym-176 preprohormone (SEQ ID NO: 36) -4.17 Hym-355 preprohormone (SEQ ID NO: 37) -2.17 Ferritin (SEQ ID NO: 64) -2.08 Potassium channel homolog (SEQ ID NO: 24) -3.33 Ropporin 1-like (SEQ ID NO: 65) -2.44 Calcium channel, voltage-dependent, alpha2 / delta subunit 1 (SEQ ID NO: 43) -2.22 Calmodulin (SEQ ID NO: 23) -2.04 Cholinergic receptor, nicotinic, alpha 9 (SEQ ID NO: 45) -2.33 Deleted in colorectal carcinoma (SEQ ID NO: 46) -2.44 TNF-receptor-associated factor 1 (SEQ ID NO: 48) -2.22 EGF-like module containing, mucin-like sequence 4 (SEQ ID NO: 66) -2.38 Periculin (SEQ ID NO: 51) -3.13

The hydra gene corresponding to the benzopyran exposure proposed in the present invention may be usefully used for the detection of aquatic ecosystem stress and a method of health diagnosis through differential expression genes which are changed according to the benzopyrene exposure time.

<110> Korea Institute of Ocean Science and Technology <120> Benzo [a] pyrene responsive genes in Hydra magnipapillata and the          method for diagnosing aquatic environment pollution using the          same <130> 13p-02-14 <160> 66 <170> Kopatentin 2.0 <210> 1 <211> 653 <212> DNA <213> Hydra magnipapillata <400> 1 gaacttgggt tttgttacta tgaagaggaa tacagtgaag catttgatac aggttttgtt 60 gacatacctt ctggaaatga agatgctctt aaagaggctg ttgctacaat tggacctata 120 tctgttgcca ttgatgcaag taaaaagtct tttacgttct atgagtcagg tgtgtattat 180 gagcctactt gtggaaatgc ttttgagaat cttaatcatg ctgttttagt cgttggttat 240 ggtaccgaaa aaggtcgcga tttttggctt gtaaaaaaca gttggggtaa aggctgggga 300 gaaaaaggtt acattaaaat gtcaagaaat atgacaaacc aatgtggcat tgcaactaaa 360 gccagttttc cgttagttta aattttaaaa taatttttat cgtttatatt tgtttggaca 420 cctttgtaca actatatcaa cagttttaaa tatcttggga gaaaattggt aaaaaacaaa 480 caaagccaat caacgtttag actggcaaaa atggtgtcgg aataagtctt caagtgataa 540 cagattgatc ctagaaaaag atatcagaac tattatttaa tgatgcacgc aatatattgc 600 tttttatatt cattttgaat taacttttta tataataaaa tttttattta caa 653 <210> 2 <211> 2436 <212> DNA <213> Hydra magnipapillata <400> 2 ctacctgtct gtaacctttt gcagatcaag taattcttca aacttgtaga gttctttaac 60 acgacttctt gcgaaataag ctatacatta aaaaatggtt gaaggtggtg aaattgaaac 120 tttcgctttt caagcagaga tagctcagct aatgagcttg attattaaca cgttttactc 180 taacaaagaa atttatctgc gagagttgat ttctaatgct tcagatgcgt tagacaaaat 240 ccgttactta tcactaaccg atccgactgt tttggattct ggaagtgaat taaagatcga 300 cattatacca aacaaggagg aaaaaaccat cactattttt gacaccggta ttggtatgac 360 aaaagcagat cttgttaaca atctcggcac tattgctaaa tcaggtacaa aagcatttat 420 ggaagctctt caagctggag cagatatatc catgattaga cagtttggtg tcgggttcta 480 ttcagcatat cttgttgctg ataaagtgga agtgataaca aaaaacaatg atgatgagca 540 atatatatgg gtttcttctg ctggtggctc atttactgta caaagagaca cagtcaatga 600 accacttggt agaggaacaa aaattatact gcacatgaaa gaagatcagc tagatttttc 660 cgaagaaaaa aaagtaaaag atatcattaa aaaacacagt cagtttattg gatatccaat 720 caatttgaga gttcagaaaa ctagagacaa ggaggtttct gatgacgaag ctgaagatga 780 agagaaaaaa gataaatctg aagaaaaaat ggaggatgaa gatgaagatg aacctaaaat 840 agaagatgtt ggtgatgatg cagaagctga gaaaaaagac aaaaagaaaa aaaagaaaat 900 aaaggaaaac tatactgaaa tggaacaact caacaaaact aaaccgctgt ggactagaaa 960 cccagatgat attagctctg aagagtatgc tgatttttac aaaagtttga ctaatgattg 1020 ggaagagcat cttgcagtaa agcacttctc tgttgaaggc caacttgaat tcagagcaat 1080 cttatttgtt cctaaaaggg ctccatttga cttgtttgaa aataaaaaac aaaaaaattc 1140 aattaaatta tttgtcagaa gagtttttat aatggaaaat tgtgaggaag tgatgcctga 1200 atggcttaac tttgttaaag gtgtggttga ctctgaggat ttgcctctta atatttccag 1260 agaaatgttg caacagagta aaatattaaa agtcattcgt aagaaccttg taaaaaaatg 1320 cttagaactt tttgttgaga tttcagagga taaagataat tataaaaagt tttatgaaca 1380 atttagtaaa aacattaagc taggtataca tgaggactcg caaaatcgtt ctaaagttgc 1440 tgatttattg agatatcatt cttcagcatc tggtgatgat atgacatccc taaaagacta 1500 tgtttctcgt atgaaagaga atcaaaaaga catttactat ataactggag aaagcaagga 1560 aattgtttca acttctgctt ttgttgagaa agttaaaaag aaaggttttg aagttctata 1620 tcttattgac ccaatagatg agtatgctgt tcaacaactt aaagaatatg atggtaaaaa 1680 attagtttgt gttactaaag agggattaga attgccagtt agtgatgatg agaagaaaaa 1740 acaagaagag ttaaaagcta gttttgagga gctatgcaag gttataaaag atatattaga 1800 taaacgtgtt gaaaaagtca ctgtctctaa tcgcttggtt gattcaccat gttgcattgt 1860 tactagtact tatggatggt cagcaaatat ggaaagaatt atgaaagcgc aagctcttcg 1920 tgacacaagt acaatgggtt acatggcagc taaaaagcat cttgaaataa atccagagca 1980 tagtataatg gttgccctta aaaagaaagt tgatgcagat aaaaatgata aatcaataaa 2040 agacttgatt gttttgttat atgaaacatc acttctgtcg tcaggttttt cgcttgagga 2100 tcctcaaaat catgctgctc gcattcatcg tatggtcaaa cttggtctag gtgtcgatga 2160 agatgaaagt gccgtagagg aaatggcaac agacgatgtg ccacctctcg aaggtgatcc 2220 ggaaaaagac gaagataaag ctagaatgga agaggtcgac taaaacgcta tgttctgcaa 2280 aatgtaattt caacgtgtgg tgtatccaaa tggtccccta ttttttgtac agtttttagt 2340 taacccgtag gtgagattca atttattggt ttcacgtgta aataaatcac taaaatagaa 2400 tttttaatta gaagacctat aaaccaataa atcaac 2436 <210> 3 <211> 768 <212> DNA <213> Hydra magnipapillata <400> 3 atgtcaaaag caccagcgat cggaatcgat cttggaacaa catattcttg tgttggagtt 60 tttcaacatg ggaaagttga aataattgca aacgagcaag ggaatcgtac aactccaagt 120 tatgtcgctt ttaccgacac tgaaagatta attggagatg cggcaaagaa ccaagttgcc 180 atgaaccctt ccaacacagt gtttgatgca aaacgattga ttggaaggaa gttcaacgat 240 ccatcggtta cttcagatag aaagcattgg cctttcaatg ttatagatga aggttcaaga 300 ccaaagattc aagttgaatt caaaggagaa acaaaatctt tttacccgga ggagatatcg 360 tctatggttt tgctaaagat gaaagaaatt gcagatgctt acctcggtaa gaaagtcaca 420 gatgtcgtaa ttaccgtacc agcttacttt aacgattcgc aacgccaagc aacaaaagac 480 gcgggagtta ttgccggatt aaatgttcta cgcattatta acgaacctac tgcagctgct 540 attgcttacg gtctcgataa aaaagtggga aaagagaaaa acgtgcttat tttcgatttg 600 ggtggtggca ctttcgatgt ttctatactc gccatagaag atggtatatt tgaagtaaaa 660 tcgactgcag gtgatactca ccttggtggc gaagattttg acaatcgtct tgttaaccat 720 tttgttgagg agtttaagcg aaagcataaa aaagacattt ctggcaat 768 <210> 4 <211> 708 <212> DNA <213> Hydra magnipapillata <400> 4 aaagattcta agaaaaacaa taatatgcct aaatacgtat taaactattt taatttccaa 60 ggcaaggctg aggtagctag acttttattt cattataaag gtgtggagtt tgaaaacaac 120 ctaatcacct accaagattg gccttcactg agaagcaatc acgcaagatt tcctttggga 180 gaattgccta cattagaaat tgatgggcat gttgtttgcc aatctggtgt tataaattta 240 tatttggcag aaacatttgg tcttaacgga gcaaatgctt ctgaaagatt agtcataaat 300 caagtatgtg aaactatgaa tgatttttgg aatgattatg ctcgcattta tagaaacagt 360 acattagaca gtgagcaaaa gaaacaagct tatgcagata tacttacaaa cgaggcagct 420 aagttaaagt tatcttttat tgagagttta ctgaagcgta ataatgacgg ccatggttat 480 tttgttggtg attcgattac tcttggagat ttggtatttt tccacgttac aggagtggtc 540 gatgaatcat ttttaaatga ttatcctttg ttaacagagc tgaataatcg agttagacat 600 tgtactgaac taaagtcata tttggattct agaacgcatc cccgattacc ttaagttttt 660 ataaaatttt tgaaatcgaa tttttttgcc ttgatttaaa tgtattag 708 <210> 5 <211> 582 <212> DNA <213> Hydra magnipapillata <400> 5 gttactctta aagtagaagg tcaagttctt gaagtcagtg gaaagcatcg taacgaaaat 60 gaaaatggat ttgagtctag tgagttccac agaaagtaca ctattcccga tgacgtggat 120 gcgacagccc ttacgtcaaa catcagtcaa ggcggcgttt tgcatataga agctcctaaa 180 aagctccctg catcttctgg agaatcaacg aaagagactt ttaaattctc tttagatgta 240 caaggcttca agccagaaga aatttcgatt caagtaaaag gtagagattt agtcgttcat 300 ggtgaaacaa aaactgaaaa cagcggtgaa cacggttcaa gttaccacca caagcagttt 360 accaaacacg tagctttgcc agatgatgta gatccatccg aattatgttc tcgctatacg 420 aaagactcta aattaacgat cgaagctccg cgaaagcaac tgcaagctcc gcttaaactt 480 gaaatcaaaa tggagaataa agtggtgtct tttacaattg tttgtagttg tgttgtagct 540 atttgtaatt ttatttttta cactaatagt atgttacgct aa 582 <210> 6 <211> 1626 <212> DNA <213> Hydra magnipapillata <400> 6 atgtcaggag caataaatgc aatcaacaca tttgcaaaca cgataagtgc aactaaacga 60 cctccactaa caatagtagt actcgataac cataatagtc aaattgaact gttaaacaag 120 cgcaacaagc ttagaacaat ggaaggctgt gacaacgtgt acattgatat agataaaaca 180 aagacagaac gagacgtaga aaagagacta agaattgaaa gagatgagcg ggatagggta 240 tttgttaaga tattttgtta ccaggaaatt tgcgatgacc cacaaatgtt tgttgatggt 300 gcaagtagtg ctgacgttac ccagggtaga gttgggaact gttggtttgt tgcagctgct 360 gcatcactgg ctcttgaaaa agatctttat actaaaaact ttatcctttc aaaagaatac 420 tatcatggca ttttcagatt tagattttgg cggtttggag aatggattga agttgttatt 480 gatgaccgtc tacctactat taacggtgaa ctattatctg gttcgtatga agctcttgaa 540 tctgggaatc ctggtgatgc tcttgttgat ttcacaggtg gagtaagtga aagaatagat 600 ttagttgaag gtgattatca taataattta gaaaagaaga aattgttaca tgtaaaatta 660 agcaaattga tgtcacggaa gtctatgatt agtgcttcaa tccaggttca aaatcgagat 720 gaaatggaat tacaaatgga tcatggttta gttaagggac acgcatatag catcttggct 780 gtaaaaaaaa taaaaattgg aaatgaccat atctcaattt ttaatgccaa taggctgttc 840 atggtcaaac ttagaaatcc atggggtgac acagagtgga atggtccatg gtctgatggc 900 tccccgcaat ggaatttgtt aagtgctcaa gaacgagaaa agtatggatt agttaatgaa 960 aatagtgggg aattctggat gtcatttggc gacttttgca agtactttac aactgtgact 1020 gtttgcatat tggtaaatac atcagttatt tcttttaata aaacatatca tgaaggttca 1080 gtgaagagta aatgggaagg caagagtgct ggtggctgca ttactaataa agaaacattt 1140 cttaataatc ctcaggttga agctaatcgc atctacagat tacatcaata ttatgaccaa 1200 gctggggata gtcattttag taatgcaagg gaaatatttg ttagatttga tcttgaccct 1260 ggtagatatg ttatgatccc ttctacattt gaacctggta tttttggata ctttttactt 1320 cgtgtgtaca cagatcaaaa caatgctttt aggcagttaa ctgaagatgc accaaaacct 1380 ggatgtttga atccatattg cattattgaa tgcaatggtt taactctgta cacacctatt 1440 gtgtatgata gtctcaatcc tacctttgat tatggtggac ttttttattt gagtaaacaa 1500 gtgaaaccaa tgctgaaaat ccaggttttc cttatattta tcaaaatcca aaataatttc 1560 agaattggat atgctgagtg gtggtttgct gtaaatgatt tacctttgtt tatgttccct 1620 tcttaa 1626 <210> 7 <211> 1428 <212> DNA <213> Hydra magnipapillata <400> 7 gattatctca gtgactttgg ttattacaaa cgaaacaaca aagatctagg ttcaatgtca 60 ggcaaaaaag aagttcaaaa ttcggtcgaa agttttcaaa gatttctagg attacctgtc 120 acaggagtta tagataaaac aacacaggaa ataatgaaaa aacctcgatg tggattaaaa 180 gatattcttg atgaaaaggt taatagaaag agaaggttta ctctccaagg aagcaagtgg 240 agtaaaaata atatcacgtg gaaactttta aacgataata atgatggatt atcacgcaaa 300 gaggttgaaa ctatactcga aaatgctttt gcaaagtggg aggctgttgc aaatttaaag 360 ttttacaaac tcgattttac aagtaaagca caagcagata tagatgttaa atttgtacgc 420 ggtaatcatg gtgatggtat tccgtttcag ccttggcatt atatatatgc acatgcgttt 480 ttccctttaa acaatgaagg gtattctggt gacgttcatt tcaacgacgt atataacttt 540 aatgttaaag aaagaaatgg tgttaactta tattgggttg ctattcacga gatcggacac 600 agtttagggt tatatcattc aagtgtccaa aattctctta tgtaccctta ttataaaggt 660 actaaggacc ttaacatcca gcttcatgaa gacgatatct taggaataca gaatttatat 720 ggatcgaaac ctgtattaac atcaaaaaag acaccagaca caaacaatac gcaagtcaca 780 caaaataaaa cacatacgac aacttcaaag aagcatatca atattcaagc tatcatctat 840 gaccagttaa ctgggatgac ttatgtattc aacaaagata agtattacaa gctaagcaaa 900 aatttaaatg aaacgcatgg accttttgat gtttctaaat tattaacaaa gataactttc 960 gttaatggag ggttcatgaa tcgtgaaaaa aaattagtct tttttcaggg aaccaaatat 1020 tatatgtttg aaagttttac aaatacaaaa tttattggca gtggttccat atttgactta 1080 tttaaaggga ttaaaaataa tgttgaaaaa attgacggag catttgttgc agataatgga 1140 actacttatc tctttactgg tactgaatac tatgaatata gttccaaaca aaaaacaatt 1200 gaatcatcac ccaagaaaat agctgagtac tggaaggtgc ctacaaatat tgactctgtt 1260 tttgtttggc gtaatgcaga gatctacttt tttaaaggtt tacagtttta tcgagttcat 1320 caaaatggcg gtttaatgaa gggttatcca aaaaatattg atggcgcatg gttgaaaatt 1380 cacaagaacg tttaatacta attttgtaat taattttatt cttaaata 1428 <210> 8 <211> 823 <212> DNA <213> Hydra magnipapillata <400> 8 atttgtatta catccaagat tcagactgtg tttttttcgg attacaagta attcaaagca 60 gaaatgaagc taataattgt gctagtaatg atgttggttt gtgtgtacag catgagtatt 120 gaaaagaata tacctaagag tcacgatgta ccacccaaaa gacaagttgc agaaactaaa 180 gttgaaaaaa agaaacgtag cgatgatggt gatgaggaaa tatgcgatga cgatgatgaa 240 gattgcgaag acgtagtaga tatagaagaa tgcaatgaag atgatgatga ttgtgaagag 300 gaaaaaaaga aaaagaaaag ggaaactaaa tctaaattaa agaaacgtaa cgatgacgaa 360 gaagaggaag aatgcgaaga agatgacgaa gattgcgaag ttgaagtaga tatagaagaa 420 tgcaatgaag atgatgatga ttgcgaaaat ggaggagaaa cagaagaatg cgatgaagaa 480 gacgatgatt gctatgatga agataagaag aaaaaaagaa aacaaactga aaaaagaaag 540 taagaagaaa aattaaaaaa aaacagtgtc aaagaatgca aagaaaagtt ctaaaagatc 600 aacgagtaca aaaaagacca gccaaaaaaa gcaacaaaat aaaagaggcg caattcaaaa 660 aaacctcaaa gataaaagaa gcaaacttta aaaaaaaatt caacctgaat ttttaagtaa 720 tgtatttttg taaaagttac ggcgtttttt aatattatgt ttattttttg attgcgtttt 780 ttgtagctct actttttaaa gccaaggtag agttttcagt ata 823 <210> 9 <211> 861 <212> DNA <213> Hydra magnipapillata <400> 9 tatatttaag aaaccctaac ctcaagtaaa caatctttgt catcagaaca tgaatcaaca 60 gtaacagatg aaaaacctaa tcaagctcaa tgctatccta tgacgcgtaa aaaacttggc 120 aaacttttca tttttaacaa tatgcacaat gataaaaaga ctactcatag acttgcagca 180 aaatatgatg tcaaaaaaat taaagcaacc ttcagcaaaa tagaatttga agtcgtagtt 240 tataaaccga atagctttga agagatgaaa aaaaatttag aagaagtttt aaaggaatca 300 tatggcgagt acaatatttt ttcaatattt ttcattgctc atggacagga tggtaaacta 360 tttggaccaa atgaaactaa taacaaaaaa tcattttcaa tacttgaatt gaaaaaaatg 420 cttgaggata actcttggct gaaaaatatt cccaaactgg tcttttgcga aagctgtcgc 480 ggcttcgata aagattttca acaaatgacc ctaactaatt ttgaagctaa atctaatgaa 540 aagaatgtaa atacggcaaa atttgctgac ttatttattg gtttttctac tgctgagtct 600 tttgtatcaa tatctatgcc ttcagataat gatgttcaaa tgaaaaactt ttacagcccg 660 tgggtcattg aactttgcaa acaaattcag gaaaaataca tgacaactga ctttctacag 720 atttaccagg acctccagcg atacatgagt agcacacctt tattgtctaa tggcgaagaa 780 gtttatttcc agatgccaga acttcaatct actttaagat ataaacttta tcttaatatg 840 gagagaaaaa cttaaaaaaa a 861 <210> 10 <211> 710 <212> DNA <213> Hydra magnipapillata <400> 10 ttttatttga tctatttagt atattttatt atatctggtt caatttatat aagttcattt 60 tttggtatgt gctttacata tggccgatcc taacaataca aatattgcca caatactaaa 120 aattagaagat aaagaagctg gtgctagttt tgctcaggtt attgatattg acttaatcaa 180 caacaaagac aatgaagtac atgatgttca tttttgtgat gtttatttaa atgatgtttg 240 ttcaagacat cttaataaag atttattaaa aattgaagac attcaaaaca aagatattgg 300 ctccaatatg 360 ttctttaaaa cctctaggac tacctgacat tgatattgta ctaaatgaac cacatgatcc 420 cactaaagct ttaagtcctg gagaagctct aattactaat gacttcatac ctagttatat 480 gaagccaata agaaaccctt tagaaaatga taggttcaaa ggaaatgatt ttaataaaag 540 atcaggtgat agaaatactt tcatcattga tctaccaata gtaaatctgc aaaatttaaa 600 aaaagtaaac tcttctaaaa tgctgaaaat tttgacagaa gagaaagaat taagagaaag 660 acctgtgtac ataggacact tttatcaagg cacaaaagct gtgactctag 710 <210> 11 <211> 600 <212> DNA <213> Hydra magnipapillata <400> 11 atggccgatt ccggttaccc ggttgctgga ttcactttaa ttgttgtcgg catattaaca 60 aaagtttatt ccgaatatta tatactatat tctgacaaca aaagtgaacc gttaccgtgg 120 cttattattg gcgttggtat taccgtcttg cttgcaggaa tactaggttg ttgtggtgct 180 attagaggaa acatgtgtat cttgggaatt tttttaatat tgcttttgat tatatttgtt 240 tttgaaattg ctgttggtat tatggcattt acctattcat caaaggttga agcttatgtt 300 gataaaagat taaaaattgc tgtagaagat gtttttgata atagttcttc atcaagagat 360 tttctcgatt gggctcatga aaagttcagt tgctgcggat atttaggacc ttctgacttt 420 aacaatgcta caggttcaaa gttgacatgt ggatataata atggtcttcc atcatgccat 480 cgaaagatga attgccaaaa aactcttttt accgatgggt gcaagttaaa gttttatgat 540 tttattaaac aaaatatagt tattgctggc ggagttgttt ttggtctttc ggttatgcag 600                                                                          600 <210> 12 <211> 447 <212> DNA <213> Hydra magnipapillata <400> 12 acatataagt gttagatatt ataataacaa aatggtgttt tttacgtcta tgaaacttgg 60 cgataagtta gctgttgctc cactccttgc gtgtattggc acagcaatga ctttagctgt 120 tgcatcatca attcgagcct ttagagcacc tgatgttgta ttatctcatg caaaaaatcc 180 aacaccttgg aatgagatat ctccaacgca gcaagtaaaa ctgtttagct ccagtgacta 240 ttctaaactt gaacccgttg cacctaaaga agctttcgat gcattgaagt aattgattgc 300 gtacattaaa gtaaatgttt ttttatatgt gatgtaatat ttttttaaat aatattgttt 360 taaataatat tgtacaaaaa attaatcttt gtatatttgt aatataatac gactcgcatc 420 tttagaggaa ataaatatga tatatga 447 <210> 13 <211> 1330 <212> DNA <213> Hydra magnipapillata <400> 13 tagcgttaaa ttaaagttat tgaatatgaa aagaaaaaca aaagaaacaa atgttagagc 60 caagcaaaaa aatgatttac cagaagattc aagaagaaga tcatcaagaa aaattgtgaa 120 ttatgcagaa aacaatgaag aagaaagttc tgaatctgat tttgagccag taagtaaaaa 180 aataaaggct actaaaataa aaactccacg aaagaataac acagtggcta taaaacctgt 240 ttcggaacca tcaactaatg ttcaaatttt aaatataact aacttacata ctgctgtaaa 300 cctttcagag gatgaaagtt cttcagatga tgatgtccca ctctcagcaa tcaaatcttg 360 tatcctccca aaagtgaatg aggcaaataa accttgtatc ctcccaaaag tgaatgaggc 420 aaataatttt gaagctgtga atagcgcatt ctttatcgaa ccaaaaacag aaattaaaaa 480 tgaaataaaa gaaactcttg aagataataa agttttagat gataaaaaaa attctttgat 540 cactaaaaaa gcaggcagtt taaaaaacag caaaacaaaa aaatctatac ctatagagcc 600 aaaaaagttc aaaaaacaga aaacaacaac accaaagtat taagagctcg 660 taaagaaaat aaagttttaa aaatagaaac tagtaagtca ttggttgaaa taaataaaaa 720 gaaagtaagc actagcttga aagaaatctc tgtgaacagc tcaaaagcga ataatatgaa 780 gttccagagc gatacatcaa gcttagctga tagtgaagaa gaaaattggg aagaagtaga 840 ggaaattgaa gtaaagtcca aatcaaagaa aatgaaaaag gaagaaataa aagaagataa 900 cataaagtta aatgatctcg agataagcgt aggagtctct tctatccata aaaagaagtt 960 aacaagacaa gagaaatatc ataactgggt gagacagtta attggtcgct ttcaacgaga 1020 gttgcagcaa catattcata aaattcatct attatgtttg tttgcttgtg aaataaaaag 1080 aaacatggtt tgcaatatta aatctgtgca agccatttgt ttgtcattgg ttcctttgga 1140 atttacaaaa attttaacac gacaatggaa catcaatcac ttgcggaagt tcatagactg 1200 gtatcagaaa gaaatacctt ctactgaaga actgttactt tttactgaca aggcaacaca 1260 aattcttgta atcatgttgc gatctcttgg atttttgtcc cgaataattt gttctttaca 1320 agtaaaataa 1330 <210> 14 <211> 713 <212> DNA <213> Hydra magnipapillata <400> 14 gattttaacg attgtttcta ccaagaggaa tacagtgtag catctgatac aggttttgtt 60 gacatacctt ctggaaatga aaatgctctt aaagaggctg ttgctacaat tggaccaata 120 tctgttgcta ttgatgctag taaaagttct tttgcgagct ataagtcagg tgtgtattat 180 gagccaactt gtggcaatgc tatacagaac cttactcatg gtgttttagt cgttggttat 240 ggtaccgaaa aaggtcgcga tttttggctt gtaaaaaaca gttggggcaa aaagtgggga 300 gatcaaggtt atattaaaat gtcaagaaat aagtcgaatc aatgtggcat tgcaactaaa 360 gccagttttc cgttagttta agctttaaaa caacttcaat cattcataat tgtttggaca 420 cctttataca actatatcaa cagtcataaa tattttgaaa gaaaactgta aaaaaccaaa 480 catagctaaa gaacttttag aattataaac cagcatattt ttctattaag tattttacaa 540 agcacaataa acaatgcaca aacatttaaa aatggtgttg gaataagttt tcaagtgata 600 acagcttgat cctagaaaaa gatcccagaa ctattattta aggatgcacg caatataatg 660 ctttttatat tactttgtat taacattttt ttttaataaa aatttttatt tac 713 <210> 15 <211> 3147 <212> DNA <213> Hydra magnipapillata <400> 15 atggattcag acaatgctga aaattataat gacaatgttt attcactaga aataaaaaaa 60 gtttctcagc taaatacctt aaatgtaaat catattgctt tgttggcgtt acaatttggt 120 acaagtaaca gagcaactgc atcaataggc actgcaacat taaaagcagc caaagatgta 180 ggatttttga aagatcatat acttgataaa cacctagctc tagatcataa aaaaattgag 240 agattaaagc aaagagtaat gaaggtctgt aaaaagagac atctagtaaa gcaagagggc 300 atacaatgta taatgttcga tggtagaaaa gatcttacaa aaacagttgt gtatggcaat 360 gatgcacata acgttgaaaa ccaattagtt gcaattggag gagatttaac taaccttaac 420 acagaaaaaa aagttattat aggttggaaa ggtggagtta ttcaattttt agagggaaaa 480 ctaggattca aacttatctg gattatttgt gcgctatata ccaacaaatt gccaattagg 540 cttttaaagt tagctttaga tggaaaaact ttatctgaaa atagattctc aggaaacatt 600 agaaaacttg ttaacatagc aacaaatcat cctattttga catacattcc tcagcttgat 660 tttgttgtag acctaattta tctcgagaag gaagttgaac attataacaa atgtgttgag 720 tttaaagcaa agtatattaa aagagaaaca gagttgaaag agaaactatc agaagaaaaa 780 aaaagcttag aagaattaga gtggagattt gaaagttata aaaaagcagt ctcttcaaac 840 aatatttcat tagcttctaa ccaaacggaa ttacatagca tcatagagaa tgaaaaaaag 900 aatcttttta agacgcttca aagcattgaa gagaagaatc ctgaagatag tgctgttgag 960 ccccttaaca aggctcttga ggttttgcag tctttttcaa gtcataataa ctcactttca 1020 acacatgata gctctgaatt cattaaaatt gatgaccttg ttagtcaaat tactggcatc 1080 gaaggctctc ttattgacaa tccgtcttca catacttgcg aagtaaaaga tttgctttcc 1140 aatacagtta agtctattca atttttaaat ccgcatcaag gtagtaatca gccaatttct 1200 ttagaccatt attatgattt aaacacaagc tatgatgctc taatgatgga gaaaaacaaa 1260 atcaaattag agtatgaaga acttagtgac aattataatc aagtactaaa agtatcagaa 1320 cagtattacg ctgtaaagaa ggagaattcg gaaatattgg cagtacttaa agaaaaaaat 1380 gagcatatac tttatcttga aagccaaatg gccaatagat ctgttatcat tgaaccattt 1440 aacggggaag aattaaatga actaaaaaat gaaaataggc aattgaaaaa cagaattaaa 1500 gaactggaac ttgagaactc aaatgaagaa ttaaataaac taaaaaatga aaataggcag 1560 ttgaaaagca gaattaaaga agtagaattt gaaaactcag aaactactaa atcattttct 1620 gctgtggatg aggaaaactg tcaacttaaa gaagaaaatg agcagctaaa tgttaagaac 1680 aagcagctaa tagcagagaa caagcagcta atagttgaga acaaacaact actgatcgag 1740 aacaagcagc taatagttga aaacaagcag tataaatgtg aaaatgaaca gcttaaagtt 1800 gagaacaaaa agtctaagct tgaggatgag cagcttaaaa atgagaataa tgtatatagt 1860 attcaaataa atgaaatgaa ggaattgatc aagctacttc agcagggtaa aactgattta 1920 ccccaagtgc gcaataatca gagcgtctat gaccatcttg tattccaaag caataaatca 1980 aaagaagcaa atgtacctga aacacaaata agtgcaaact acattattag tcaacaagca 2040 gaaaatcaaa tgatgacaag gataagagag ttagaagaaa tctgtgacaa actcaagtgt 2100 caacttgatg aaaaaactca agaatgtgtc aatgccaaaa aggaagtaga gtataaacat 2160 aatcaattaa tggattttaa tgaccgaatg cacaaaatca atgcaagttt ccatgatact 2220 aacataatga atcaaaagtt aactagcaaa ttagaaaggt ttaatgagtt agatgagatg 2280 tgtgcaaagt ataaacaact ccttgaagac aaaaaaaaag aacatgcaac tataaataat 2340 gagttaattc aactaaaaga tcatataata aaaattcaac tttttccaac ttgtctttca 2400 attattagaa aaggatttac tcaaaatagt tctttagctg tacttaaaag taatgtaatg 2460 ggaaatctcg caaatgatgt caagacaatt gcacaaatag cttatactgt aataaaagat 2520 aaaattgatg aagaaggtga agaaaaagag caagttgaaa acaaaattcg agaaaaagat 2580 aatgaaatat gcactcttaa agaacaaatg gacgctttaa atgtcacaat tcagaaaatg 2640 agtgctgaag tcgattcaat acctgtttat caaacacaaa tggatgccta tgaaaatgat 2700 tttagagctg aaagggcagc gcgagagaaa atccatcaag acttatgcag gattaatgag 2760 cagctcgatg aacatatact tgagaataca agattaaaag aagaaattaa acatctcaca 2820 agcagctcat tgtcagatgt tcagcaacgt tatagtaact cttctatgac aggtagattt 2880 aatcagtcta atccacactt tttcaactcc gacaggtctg ctccgttagg aaattcaaga 2940 aatggctcta ataatcattc ctatgagact aatcaaagtt attataatga tgaaccaaac 3000 tattttaaca gaagaaagcc ctttgaaaat gttattaatc aaagaccata ttatcattct 3060 gggaacaaa aatgccccaa gtgtaatcaa ggttttcctg atttagatag tctgcaactg 3120 catgttgttg agtgtttaga taattga 3147 <210> 16 <211> 1146 <212> DNA <213> Hydra magnipapillata <400> 16 atgaattcca actttttttt cattttattc tttaatatag gtctagtggt gtgtaaagga 60 aaagatgtga atcaaaatat agatcaaaac acggaaactg ttttaccaaa atctgaaatt 120 gcaaaaaagc aaaacgagct aattgatgtt ctaaaaaacg ccaagtcgac ggaagaagtt 180 cttattggct atggagtacg tgacctttta aagttaatca aaagatctcc tataactggc 240 agttcaacat acgggatagc tccaaaacct gggcaaagtt tagatagtag tacatcagtt 300 gagtttactg aatatgaatg cgcaatacga ccgcgaattg ttgacgtgga ctttgttcca 360 ggctacttta cctttccagc atgtatacaa gttaatgaat gtagtggttg taccacaaaa 420 tcattaatat acaaatgcgt tccagacgaa caagttaatg ttattgcaaa cgtagtggac 480 attgattatt ccggaggttt cagaattaga aaagtaacca cgctgcaacc aaaaagctgt 540 aaaatggagt gccgtataaa ggcttcagat tgcacgccag gtcaagtgta tgatccaaat 600 agctgtacat gttcttgttc ttcttctatt gagaagtgtc cagttggaaa acagtggtct 660 attgtgaact gcgcttgcgc ctgttccgaa gtaaaaacat gttcttctaa caaaatatgg 720 aataaagact catgcacatg tcaatgcatt ccaaaagtat gtcttccgtc acaaaagcaa 780 gaccttatct catgcaattg tgtttgaatg ttaattgatt aaaacctttt tatataagta 840 cagtaattga aaaaagattt ttttttattg ctgtttgatt attcgataac ttaattagtt 900 tttctaacaa tattttgtaa cttttagttg aataaaaata taatatagaa aagttttttt 960 atttttttga ccaacaaaca tatgaattaa tttaattacg gatttttttt tttcaattat 1020 tgtaaacgtt ttcggatact ggtaaatatt tttggttaat agtgttttgc acaacactca 1080 atacaataac ttggagtttt tttttatgtg tagattgcaa ctcttccaat aaatatattt 1140 cttaat 1146 <210> 17 <211> 722 <212> DNA <213> Hydra magnipapillata <400> 17 taataataaa cacctgtgct gaaatcaaga tgctcatttc acgtcttact tttctattag 60 tggtatacta tcttattgaa attgattgcg ctgcgttacc gtcttttagt gatatgctag 120 aggaaacttc acagaaaccg gatattaaag ccatgattcc tttatttaaa gaaatgcaaa 180 aaccttcaat tgaagaacgc aatcctcaga aacttttata tattctcgga aacagttatg 240 actcagaatt ttcttctatt gcaaaacctc cttcaatgaa agcacgcgaa cccagcactg 300 acgatttaga taccgacata ccttctagta tgaaaacaca cccaactatg agcgaagagg 360 tccttaatgc aaagttttct gccaaagtaa aaggtaaaaa gaaggaattc gggaaacgtt 420 taactgaaaa aatgcaaaat tatttatgga acttatcaaa atgccccgtc aagtataaat 480 ggattgactt gggtgaaaat atttttccac aatatatcaa aagaggttcg tgttcaaaga 540 agaaaacatg ctcttttcct gctggtatga catgcgaaga gtcaaaatgg aaatctgtag 600 acatattgtt atacacatgt ttgaaggagt ataactctgt ttctcttgac tgtaaatggc 660 gtcctatgcc agtcaatata ttaactgaat gttcttgtca gtgtaagaaa caaacagaat 720 ag 722 <210> 18 <211> 1393 <212> DNA <213> Hydra magnipapillata <400> 18 gcgattatgt taatttacca gaaaaaagaa aaaaagtgaa tcgattaaat aaaaaaaaaa 60 gcaaagtaat catgactaag ggtttacata gttatataaa aggctcttat aaaatgaagc 120 aaaatacaac aactaagtta gattcaactt tgtctgctgg agaataagtt taaagatgtc 180 tttatttgaa aaagaagtac acatgaaagt ttattatgtc gagaatcaac ataataatga 240 aattcgaaag ttttcagtag aaaatcgaat ggctgctaat tacaaataca ttttggataa 300 gatccgccaa gtatttacaa atttgtatga aaaagatctt gatttgtttt ataaagatga 360 agaaaatgat ttcatttcaa tctcctctga tattgaattc cagcaagcct ttgagagcat 420 caacaatggt tgccttaagc tctatgtaaa aaagaaattg acaaagccag ctcaatcaaa 480 taaagaacat attggagtta gttgtgatgg atgtaattct aagatatatg gaaatcgctt 540 caaatgcact caatgttttg actttgattt gtgctcatta tgctacaaaa aaggagagca 600 cccatcagac catgaaatgc tggttatcaa agaacctcga tcttctaagc atatgtgtta 660 cagtcagctt ccgttttcac attgctggga aagatatgca cacatgaaca cgaacaattc 720 ttgttctaat aaaaatgcaa gcaataatga agaaaataaa cctactgcat ctgatcaaaa 780 gtttggggta cctgctgaac aaatgcatgc tttggttgat tgttttataa aacagtgcaa 840 tcttaaaagc aatgaaagaa atagtgataa agatataaat agttcaaaca atgaagtatt 900 cagaaataac ggagacatga accaggctaa ttttccagat aaaaaagatg tatcattaga 960 aaaccagctc atcaataaag acaaggatct tgtaaatcct tcaaactcat tgtgtcagac 1020 taatgttgaa tccgtaccac atctagatcc aaacaatgtt tctgaagtta taaaagaaca 1080 aataaatccg cttgagtctt tgtgtcaaca aacctctgag ttgcaggttg atagttcaca 1140 agaggggctt gggaatttta taggacgctt atttagccac cttgtaactc aaaaaccacc 1200 aataaacata gccaatgatt ttgaacttgt tgataaagaa agtaaagatc atgaagaatc 1260 aaagcttgaa agatctctta gacaaatgga agcgatgggt tttgagaatg aaggcgggtg 1320 gttgcgtcag ttgttaattt ctaaagattg taacattgat aaagtgcttg atgctttaag 1380 tcctgccaaa tag 1393 <210> 19 <211> 2227 <212> DNA <213> Hydra magnipapillata <400> 19 caagcgaata aagttataca atctatttat caaaatgttt tgtttaattt attatagctg 60 aggtttaaat gtcgaaaaat attatccaac catggaatgt aaccgagatt ttaaaaaaca 120 accggtacgc ataggtcttt atgacattga agaaaccatt ggaaaaggaa attttgcagt 180 cgtgaaattg gcaaaacatc gcatgacgaa atctcgagtg gcaattaaaa taattgacaa 240 gagtcgatta gatgaatcca atttaataaa aattaaaaga gaggtgcaga tcatgaagtt 300 attggaacat cctaatgtct taaaactata tcaggttatg gaaaccaaaa atatgcttta 360 cattgttaca gaatatgcaa caaaaggaga gatgtttgct tatattgaca aacatggtaa 420 actgcaagag cacgaggcac gtcgtttatt ttggcaaatt ttgtctgctg ttgaatactg 480 tcataaacat aaaattgttc atagagattt aaagactgag aacttactac ttgatgaaaa 540 tttaaatata aaaattgctg atttcggttt cagtaattat attgaagaaa atgagctatt 600 aaagacttgg tgtggtagtc ccccatatgc tgctcctgaa atatttgagg gtaaagaata 660 tgatggacct gctatagata tttggagttt aggtgttgtt ttgtatgtgc ttgtttgtgc 720 agcacttcca tttgacggag aaacagttca cgaagtaaga gatcgtgttt tggagggtcg 780 gtttcgtgtt ccatatttta tgtcttctga acttgaagat ctaattcgta aaatacttgt 840 taaaaatccg attcatcgct acagtctaga gcaaataaag gctcatcctt ggttgtatga 900 gtatccagaa gatcgaccac ctatttataa ttcctatacc agctataatg agacttttaa 960 tggtgaattg aataaacacg tattggatgt tatgatgggt ttaggcttgg atattgagaa 1020 gactaaaaaa tcattagctg tcaatggtta tgatcattta acggcaattt accatttact 1080 gagcgaacgc ctaagaccaa atcgtacaag ttatcctgaa caaaataatg taacaactcg 1140 ttatcgaaga gccagttcga tggcagatca ggttatagtt aaaaatagtc aaggtctacc 1200 tttgtctgta acacagcatg tgattcctgc aggcattaaa atagctgata aaaataaagc 1260 aggactccaa tatgctttga atgaacttca tataggagaa gtcgaaatac ctcctgatat 1320 aataaattca agtatacctg gttgtgtgaa ggaattttct cctcctccag tcccacataa 1380 ccttttgcac cctcgacttg gtcattttag ctcttctagt acacaaaaaa atatagaaac 1440 tgttaacgaa gagggcaatg atgatactga aactaaaacc gaaaatactc cactccgaca 1500 acgtcgtgga agaaggtcag ctgttgatgc tatttatcac aatcaccgtc gtcataccgt 1560 gcaaaatcct cttgttgaaa atgaaacttt gtttgttcct tctaaccatc cattattaaa 1620 taaggaattt aatgatacac ctcctgataa tggtgaccta tccaataaac taaaaataaa 1680 gattgttgaa ccaactgttc accccgagca aacctatgta tcaatgttaa taaatcctga 1740 acaaacattg catttatcta ctgttgcgca taaatcggac cttgctttta atattggaag 1800 aagagcatct gatggtgtta atgcaccttt taaacatctg ttgtataagt cagataatct 1860 gtatttaaaa gaatataaag aactgcaaag attacaaaga agctttactc ctgaaaaaat 1920 acatcagcaa tcatgccagt ttaaagaaaa tactagctta gtacatgatt ggaaattgag 1980 tccaacatct agccaaaacg agttattgat gatgaaatta caaaaaatgc atttagctaa 2040 tatagataat actttagaaa ctgttgattc atgggatgct ccaggacctt gcaggcgacc 2100 tccaacatat cgtaagtttt caagttgtgt tttaccaatg cctgcaccaa taagaagaag 2160 gcgtgcacca gtgatagata atattttaaa taattttgat aactcaactg aaagtgacat 2220 tgtttaa 2227 <210> 20 <211> 834 <212> DNA <213> Hydra magnipapillata <400> 20 atggaacttc caagatttgt aaaacaaggc acattatgtg aagattcttt tatggataat 60 ctttttaaaa gaagatgcgc agaaaaggtt ccaagagaga aaacagcgaa acgtccaatg 120 aactcatttt tgctttgggc aaaatctgta aggaaaatgt atgcaagtga aaaccctaat 180 ctaagcaaca ctgaaataag cagactgtta ggcaaagttt ggaaagaaat gagtgaagtt 240 gagaaattgc cgtttattca aagtgcaaag tgtcttcgaa ctaagtttat acaagataat 300 ccaaattatc attacttctt taaaaaaagg aaaatcaatc aactaaataa tacaagtttt 360 tctaaaatgg cttccaaatt aacaagtaat gatttaaatg catacaaaat ttttaaatat 420 ttaaacatga gtgaaattat agaacacaaa gatttgaaaa gctttggcta ccttcaaagc 480 gtattcgcag cttatcagaa cgaaaggaac ggcaatcaaa atacattttc gcctaaaatt 540 ctttcaaaaa gcattagtaa taacttcatt tcgaatgttc aattaaatgc taaatcaaat 600 aaaaataaaa gtgacagatt tgatgaaaat caacaaacgc acaaagaaac aactacgctg 660 cattactatt cagatgaagc acaacatagc tgtgaaaact ctttcaacat caataacgaa 720 gaagatttta atcaaaataa tcaaataaaa aaaagagaag atgttattga acttgactct 780 gaacttagag aattttttat gtctcttgaa aaaggctttg attatgatga atga 834 <210> 21 <211> 834 <212> DNA <213> Hydra magnipapillata <400> 21 atggagtttc cgaagtttgt aaagaaaggc acattatgtg aagattcttt tatagaaaac 60 gcttttaaaa gaagttgcgt agaaaagttt ccgagagaga aaactgcaaa acgtccaatg 120 aactcatttt tgctttgggc aaaatctgta aggaaaaatt tttcaaatga taatcctaat 180 ctaaccaaca ctgaaataag tagagtgcta gggaaagtct ggaaagaaat gagtgaagtt 240 gagaaattgc cgtttattca aagtgcaaag tgtcttcgaa ctaaattttt aaatgattat 300 ccaaattatc aatacttgtg caaaaaacgg aaattcaatc aagtaaataa tacaagtttt 360 tccaaaatgg cgtccaaact aggaagtaat gatttaaatg catacgaaat ttttaaatgt 420 ttaaacatga gtgaaattat agaacacaaa gatttgaaaa gctttggcta ccttcaaagt 480 gtattcactg cttatcaaaa cgaaagaaac gacaatcaaa atatattttt gcctgaagtt 540 tttacaaaaa gcataagtaa taaatttgat tccaatgtca aactaaatac taaatcaaat 600 aaaattaaaa gtgacagttt tgatgaaaat caacaaatgc aaaaagaaaa aactacgctg 660 cattactatt cagatgaaac acaacatttt aatcaaaatt cttttgacat aaatagcgaa 720 gaagatttta atcaaaataa tcaaataaaa gaaagagaag atgttattga aattgactct 780 gaattaagag aattttttca gtctcttgag aaaggttttg gttattatga atga 834 <210> 22 <211> 1452 <212> DNA <213> Hydra magnipapillata <400> 22 cgcattagaa tctgactagc attaagtttt aaggagtgag cagacaacat caataaaggt 60 ttttcttgaa aaagaaaaag atttatcttg tgcgcgatga ccatgtccga tagccccttg 120 gatagtccat taaacatgaa ttctggagca ataaagtcag agactccacc taaaagacgg 180 cgtgaaagga ccacttttac aaaagcacaa cttgacgtgc tggaagatat gtttggaaaa 240 actatgtatc cggatgtatt tatgagagaa gaagtagcta aaaaaattaa tttggctgaa 300 gcccgagtac aggtttggtt taaaaatcga cgtgcaaagt ttcgacgctc tcgtgaaaac 360 cctggtcgac attttgataa taataagtat ttagggcgtg agaaattaat gcctgaagat 420 ctcattcgaa cctttcctaa aagtagtggt ggcactccac actcttaccc aaatacaagc 480 aattatcctc cgctatggaa atatccaggt gcagaccaca gtccaccata tgcaggaaat 540 aatataattg aagaaccggt ttatagacaa tcaaaccctt acgctcccat ctcaccttat 600 gaatataatt ttcctagtgg gagaccacca tactttactc agccacctta ttatagtccg 660 tctgcaccta gtcaagatta tctttcaaat tccgtttcgg cgccatcaag cacaaatagt 720 atatcaagtt tatctttttc gcaacatgac actaaaaatg aattaaatct tcctgcgtct 780 tattggagtg gagcagggta tatgggtgcg aatatgtagt ttaattataa agcacctcaa 840 aaatggaaat ctatttcaga tagacacaac aaaattgatt taaaaatcaa acagttgtac 900 atagaaaagc tttttttaca attcattaaa aaaagatatc ctttagattt aagtctaaga 960 ccaatgaacc aatcgattgc gtaaattaca aaactaattg gttgtgtcta gaaaacaatt 1020 gtacagattg gtagtttcaa aatattacta ctggactaaa acattagaac aactcacaag 1080 ttgttttatt tgcattttta aaatatatat accatgtttt aaatttttat cgtagaacta 1140 ttatgcagtt atttttgttc aactttttat ttatgtataa atcgatatga cactgctata 1200 ttaccagtga aaatgtcggt ttagcaaaat ttcaagcctt tatgaaaagg aaaatggctg 1260 aaacaatgat ttttttaaca attttgtttt aacaaattta ttttgttaat aaaaaaaatg 1320 tagcagcgat caaaaatgga aaaatatatg ttattgaaac aactttttaa ttttctattt 1380 atcaattaac tgatttaacg tataaacctg ttttagattt tttttgtaaa tatattatta 1440 ctgtaataga aa 1452 <210> 23 <211> 1220 <212> DNA <213> Hydra magnipapillata <400> 23 attaaaataa atttttcatt atgttgttca tcaaacttta attttatcgt tatttaataa 60 aacagctaat tcactacaag atccaaagaa cttgacaaag cttagttagt attttataat 120 ttagaagaca gcctccgtaa tcatgcaccg ttaagatgtt aatacgtttt ttgcaatagt 180 gattattaaa acaaactggt ttagctcatt agggaataat gagaaaaagg tacactctta 240 cagatgacga tattagagaa tatcaacttg ctttttcgca tcatgacaaa aacaaggatg 300 gaaacattga ctccaaggaa ctattgccat taatgagaag cttgggtgag aatccaaccg 360 aagacgaatt gatgcatttg ttgttggaga tggacgcaaa taaaaatgga acaatcgagt 420 tcaacgagtt tatagagcaa atggaaatca gaaaaaaccg tgatgacgca gctgatttaa 480 aagaggcatt cggtgctttt gatatggatg gtgacggaaa agtcagtacg aaggaacttc 540 gcgatgcatt tatggaatat gggtacaaca gcattttaga agaaattgac gagttaatgg 600 aaatatttga cacaaacaat gacggattca tcgattatga agagtttgtc aaaataatta 660 ttgaagagac gaataaagag taaccaccaa taatgggaat gaaattagta tcaactgctt 720 tgtgaaaatt gaagcgaaag atcactgacg aaactatttc tgacgaaaat attttctaaa 780 aagttgacag caagtaaaaa acaaatactt ttaaactatt tttaagaaat tccaaaatcc 840 tatatttata gagaaagaaa gataaaagaa aaaggtgact taacagcaac caattagata 900 catcttttgt cattttaata acacccaatt acaaaaacat ctattgttgt tttaacagcg 960 accaataaat ctatcttcat tttagcaaaa gaataaagat atactcaggt tcaaagacat 1020 caatattttt ttgatgaaaa atcaacgaaa agaaaaaacc aacgaaaaac aaaagtgtta 1080 taatcgaaaa gtaatatata acatcagtaa aacagtaaaa aaaacagaca aataattttt 1140 tatagatttg ttatatggtt cagaacactt tgcgtaacgg gtacagaaga gaatatagtc 1200 gttttaaatt gaaagaaaat 1220 <210> 24 <211> 1437 <212> DNA <213> Hydra magnipapillata <400> 24 atgactaact taaacatgtt ttatgaggga aaatccagaa ataataattt agatcctatt 60 atcgaaatgc tggaaaatga acatacggca aacgaagtta aagaaattat agatattaat 120 gttgctggac aactatttca gacgtaccga actacacttg aacgatatcc ggatacttta 180 cttggagatc caaaaaagcg acaacatttt aaaaatccaa aaacaaaaga actatttttt 240 gagaacata gagaatcgtt tcaatgtatt ttgtattatt atcaatcggg gggagtaatg 300 gagaaatcac ctggcatacc tattgatatt tttgtgaacg aactaatttt ctacgagtta 360 gacagtaaat taatagaaaa aatgcaactt gataatggtt taaaagaaat agatacgaaa 420 gaaaatttac cattatttaa accgtttcgc attttatggg aattttttga atacccagag 480 gt; attctatttg taatagaaac tctgccaagt ttccaaccac atgtagttat tatatctgat 600 aattcaacaa tgacgatacc aagtgtcaat gcagtatgga caaaatattc taatacggct 660 gttattgtct ggtttacaat tgaattttta atccggctga tttcttgtcc taacaaacta 720 atgttttttc taaatggtgg taacatcatt gattttttat caatactacc attctatttg 780 agtttgatta tttcttcaaa cagaacagaa acttcgatac ttcgtgtaat gcgagtgttt 840 cgcgttttta aactagcccg gcattctcga gggcttcaaa tactcggcaa cacacttaaa 900 gcttctttta atgaactaat gatgcttgtt tttttcttgt ttgtaatgat attatttttt 960 ggaagcctta tatattatgc agaaaaagac gttcctggta caacatttac gtcaatacca 1020 gactcctttt ggtggtgtat tgtcacaatg gctaccgtag ggtatgggga tatggtgcca 1080 ataacttttt ggggtaaatt gataggctcg gtgacaatta tttttggatt gcttttggtt 1140 gctttgccag tccctataat tgtttccaat tttgagtttt attataaaaa agatcaaaac 1200 agaaaaaaag tcgaaaaaga aaaaattata aaagaaaagg aaaaaaatcg ctactttaaa 1260 acttattatc gctttcttct aaacagagac tttactaaaa atataaaaaa agctcacatc 1320 agaagatatt caatgtctca aaaccctgat ggtttgtcaa ctaaacttga cgagtcccca 1380 aatttaaatg acaattgtct ctcaaaaaac aacatatcct atgctgaaga aaaataa 1437 <210> 25 <211> 1134 <212> DNA <213> Hydra magnipapillata <400> 25 atggcaaaag ttgaagacaa aatcaaaagt ccacaggagc aaaaaagaag aactatcaaa 60 cagtatcttg catatgctgg tgaaaatatt tctattcatg gactttctca tgtctttgac 120 aaacgagaaa actttatttg tcgtaccgta tggcttctta ttacaattgc agcttttgga 180 tacgctgtac aaaaagtata tgaaagtaca ttaaactatt tttcttatcc gtttagtaca 240 gctcgaatga aaatccatgt taaccaaatg gattttcctg cagtttcatt ttgtaaccta 300 aatgactttc gttacagcgc aactaaagga acaaaacttg ataaagctat tttatctaat 360 gt; atagaagaaa tgttagttga ttgtgagttt gatggtaata aatgctctgt tcataatttt 480 acagaattta attggaacca aggtgagctt tgttttacat tcaactccgg aaaatttcct 540 cattcattat taaaagtaaa tggtgttggg atgaaaagaa gtctggtact taccataaac 600 gttcaacatt atgaatacta cggtgacgaa ttagatgcag gtatacattt aatacttcac 660 gaccaagaag aaactccaat aaaaaagcgt ggacccgtaa taccacctgg atttacaaca 720 tacatacaag tcgaaaaaaa aacgatttta aacttagaat caccatacaa aacaaaatgt 780 ggctccataa agttaaagta ttttgatagt tactcaatgc acacctgctg gcttgaacaa 840 cttactgatc atgtttacaa aatttgtaaa tgtaaagact tttttatgcc agttagggaa 900 aatttacttc gtgttgttat ttattacaat gatttatcat atgagttgct tgaacagaaa 960 ccaaactatg atacattagt gtggcttgga gatgttggtg gtcaaatagg attatttgtt 1020 ggtgccggag taatgtcata ttttgaaata atagactgtt tggttctgat catttacact 1080 cgattttttc aaaagtttaa aacgataaat tctataagaa atcactcttt ataa 1134 <210> 26 <211> 1692 <212> DNA <213> Hydra magnipapillata <400> 26 atgtatccat cagattgcgt tccacttaga atctacggaa tggttaaagc tcacatacca 60 gaaaaaaact acctaatgcg tcctgttatt tccattatta acacaccacc ttatgaaaca 120 tcagagtatc tgggtttaag attgcaaaat ttacaaaata atgttcatta tacttgttct 180 ccgtgcggta agcataactg ttttgtgtgt ttaactaaat gcgaggcttg cagaaactta 240 aattgttcat gcagaacaat agagtatgga aatgaaattt cgagagtgaa accaaagcct 300 agtatctaca ataattcact tattaaagtt gggggagttg gagatttttg cgaaataaag 360 tcttttagag atgctaactt accagaagta ctaattgaaa accttagaaa gcttttatac 420 aaggttccta cacctgttca gtcgatagca ataccaatat tactagaagg caaagattta 480 gtagctcgtg cagaaactgg atcaggaaaa acggctgcat ttttaatacc aattgtttca 540 accttaatta aaagaaaaac agtgaattca atgaatccat ttgctgtaat tttaactccc 600 acacgagaat tggctttaca aattgagaga gaagctttgt tgttgacaga aaataccttt 660 ataaagccag tatgtaccta tggtggaata actattggtc atcagaagaa aaaaattgca 720 aaaggttgtg atatacttat tggaaccctt ggaagaatcc ttcatcttgc taaacaatca 780 atgctctcct taaaagaact caaaatactt attattgatg agggagatca tatgctagat 840 aaaaattttg ttaaagatat tggaaaatta gtgcatgact ttgatatgcc tgaaaaatct 900 ctacgacaga ctgctttatt ttgtgcaaca ataccgacta ctcaaataat ccatgatgac 960 aaaggagtta aaaaaaaatt agcatttttt actgatactg tttgcatgtt tttaaatgat 1020 acatactgtc atatagatgt tggtaacaat ttggttacac caaaatccat aattcaacaa 1080 tttttgtttg tagataaact ggaattaaaa tttcaattat tagaaaaaaa aatcgctgaa 1140 gaactatcaa atgaaaataa aactattgtt ttcattgaaa ccattaaaga aacaataaaa 1200 gtagctcaac agctgtgcat taaagattac cctgtaacag ttattcatgg tcggcgtact 1260 caaaatgatc ggaaacaagc tatgtatgaa ttcaatgaag gtttaaaacc tcttttgata 1320 tgtaccaatg ttagtgcacg tggtcttgat atttttgaag ttaaaacagt aataaattat 1380 aatttaccga atcttactca tgatgatatt agaacatacg tatatcgaat agggcgtgca 1440 ggtcgtattg ggaatccagg caaatcaatc tcattgtttt tgaaaggaag ggatgaagtc 1500 ctagctaaac cattggttgt tttattagga gggtctcaac aagaggtacc aacatggcta 1560 gaggagcttt gcttagttcg tgaaatggca aaattgggta actattctca agcaatttat 1620 gaaacaaaaa gggttgcaat gcttacagag aaaaattcta gtgatgatac taggaggact 1680 attttctgtt aa 1692 <210> 27 <211> 1247 <212> DNA <213> Hydra magnipapillata <400> 27 ataaaaataa gtttgcaact ggaaagttga aaaatcagta atagctatac aactttaaaa 60 ctattttttt tatgcaaaag taaaaattaa ctttttattc ttctattaac aacaggttct 120 aaaacttgat tttgacatca aacaacaaaa ggaaacaaat ggattatcaa aataagatta 180 cgagacatt caaaaaagcc taaaatgcat gaggatgggg cttccatatc aaatcaaaaa aatttgattc 300 aaatcaacga aagttgttca agtacccaaa tcagttcgga aaatataata aaaaaagaag 360 ggaaagtttt agtcaggaat gacgaggaag gtatagaatt ttcgcacata agatattttc 420 ctaaaaagcc aacaagagat ttatcgaagg ttgatttcaa gtttaactct aataactttt 480 atgatactaa cacttttcca agaggaactt taacaataat aaatgtaaac aactttatga 540 aatcttctgg tgtgcataaa gactctagat taggtacaga ggatgatgca gagattctat 600 gtaatctttt cctagaatta ggttttaaaa tagatataat taacaatccc aagtcaactg 660 atgtattaaa aatactgaag caggcagcaa atgaagatta ttcttcaatg agttgttgtg 720 tggtcgcatt actatcatat ggagaagagg gcaaaattat ttgcacaaac gaatctttaa 780 atataaggga aataacaaat ctattctgca ccaaagcttt agcaggaaaa ccaaaatttt 840 ttttagttga aagttgtcta ggaaaaaatt acatagaatc attagacagt gttgatggcc 900 gtgcattatc aaatagtgca aatcttggat taccacataa tgcaaatgtt ctaaaactgc 960 ctgaagaaag cgattttttg tatgcctatt caacagttct aggttctttt tcaaacaaag 1020 aaattggttc gtggtttatc aaagctgttg tttctgtttt ttgtgaatac gcccataaaa 1080 tggacgttgt tcaactttta acacgtgtga acgataaagt aagcgaaaaa aggtcggaaa 1140 ccatagataa agcaacaaac aataaaaaac aaatcggctt aataatctct caattgcgaa 1200 aggagttatt tttaccacca atttatggac caatggaaat cgtataa 1247 <210> 28 <211> 2382 <212> DNA <213> Hydra magnipapillata <400> 28 atggctgcag atttcagttt tacccaagca gaaagagcaa agtttaaagg gtaccatcaa 60 aagtggaatc atggacgaat acctctttat atagcattaa tcatagaaat tttatcgcca 120 gctaagttgt tatctttatc ttttcaaagc aacgaagttg attctgtagc atcttctgga 180 tttattgaac aaactaaaaa gcagttaagt cgtttgcaga aaaaagagtt taatgattta 240 ccaacagtca aacgtttgct atcaaaagta acaaatagta atggaaagta ttcatttcag 300 gatgtcgagc ttcatgattt tacaaacgca ctaactctag ttaagaattc gaaatcaatg 360 attgtagtgt taattaccaa atccatagaa gagcatttgg aggcacaaaa tacagttcct 420 gatatgtacg gaatgttaat actaaacact ggcggttggt atcaaaataa cacaaataat 480 tcatttgcag atgataacat caaaaaattg tatgactttt ataatatgca gttacgttat 540 actggcttca ctgggaatgt gaacaatatg ttaaatgctt ggcatagttt agttgattat 600 actgtcaaat atttggcatc atatacaggc ttgaaaaaag ttcttactag cccttgccac 660 aaggaagatc ttaataactt tataaataac tcacttcctg gttcaagttt gattgcacaa 720 gatttaatca aacttcctgc attttcaaat cagaacgaat ttcaagagca ttctgttgaa 780 agtaatacct tggatgatat ggatatgctg gcaactcaag catactgcat gacggaaaat 840 tttcatgata gtcaatatgc aaatattgaa acgcagacac caagttctgc cagtgcatct 900 atggagccaa atatttcaag tgctacacaa gatttctgta tgatatctca aaaatatatg 960 gatcatgttg aagtcgataa tgatgaaaac aaaggaaatg aggataatgc tgcaaacaat 1020 ggagaagaca atagtgttgc aagcaatgaa ctttgcaact ttgcaaacat tgaagagggc 1080 aaaagtgttt caaataacag atatggtgat gcaaacaagg tacttagtga tagcacagca 1140 aataaggaag atagtgataa tatagcaaac aagggagata gtgataagga agatagtaat 1200 aaagctgcat atgaaaaaga ttgtattagt gttaaaacta acgaaaatga caatagtgtt 1260 gcaaaggatt taaaacataa aaagtatgtt cacaacccaa aaaagattca aacaagaaaa 1320 tcgctaaaat catattttac tgatataaac cttgaaatga aaatttgtct ggaagaagtt 1380 gacgataata aacccaaaaa cagaagggga aaaaagtcaa tatattcttc taaaaggaat 1440 gtagaaaaag aacagcatct tatttgtgaa gaaaagaaaa taataaaaaa agaaccaaat 1500 gaatcaccaa gagaatcatg ttttacagat gttatcgatc tcggaaaaaa ccaaaaccta 1560 aaattactta agcaaaagga aacaatcgag ttttctccac aggatataaa accaataatt 1620 aaaagaaaat tgatagaaaa tgaccctgaa aagtttgtag tttgtaaaag aaaaacaata 1680 gacaaaagca agagtgaaaa ctctgttata gcaaaaaact tgtcatcaaa taaagaaaat 1740 tcatttaata atttatcctc aaaaaatgta agcaagcgtc gactttcttc ttcaaaacct 1800 aaagttgtgt ttactggtgt aacagacaaa aatattgaaa agattgtgaa agaacttggt 1860 ggtgaacttg ttgataatgt aggctcagca actcacctag ttacagataa ggttcgtcgt 1920 acagttaaat ttttatgtgc tgtagcaaga ggcataccca ttgtcagttt agagtggtta 1980 aaagctggaa aaactgcttc catgtttgtt ccacaccatc atcatttgtt gaaagataat 2040 gatgctgaac gtcaatacaa ttttgatctt gtagaatctt tagaaacagc agcacatact 2100 catttgtttg aaaatgtgca aatttacgta accaggaatg tgaagccgga accaaaagct 2160 ataaaagaga tgattgaatt ttctggtggc aagtttcttc ataaagctcc tacgaaatat 2220 atggaaaaca catacatagt ctcttgcatt gaagatcata aagaaatatt gaaatttgaa 2280 aaattgaact atagaatttt atcaaatgaa tttatattaa ctgggatatt acttcaaaaa 2340 ttttctcccg atcagtatta cttaacttag ttgatttgag at 2382 <210> 29 <211> 1703 <212> DNA <213> Hydra magnipapillata <400> 29 gctaatgagt tcaaagataa gggcaacaaa gccctgcagg atggtaactt agaagatgct 60 attgcatttt attcaaaggc tattgagctt gatagttcga attatgtttt ctacagcaac 120 agatcagcag catatgcaaa aaaaggtgat tacaacaatg cattggctga tgcaaagaaa 180 actgtggaaa tcaaacctga ttggggaaag ggttactcac gtctaggtgc tgcatacagt 240 taccttggac aagatatgga agcatatgaa gcttatgaga aaggtcttaa atatgaacct 300 gataatgcac aattaaaaac agccatgcaa gaactagata acaaaatgag tcggcagaat 360 aatccatttg cagatccaag ccttgaagca aagctggcaa tgaatcctaa aacaagagag 420 tatttaaatg atccatcttt tctgcaaatg cttcaagttt taaaatctga tccatccaaa 480 ttaagcatgt ttgcaaaaga ccagagagtg atgcaagtac taagtgtttt acttggaata 540 caaatggatt ttccccaaca gtcagaaaac aagccaaaac cacaagaaac taaagtaaaa 600 acagaaacta ccaagcctcc tgaagataaa cgtacagaca ctcagcgcca ggcagatagt 660 gagaaagaac ttggtaatga tgcatacaag aaacgcgact ttgttactgc tcacattcat 720 tacgacaatg ctattaattt ggaacctact aatattttat atcttaataa caaagctgca 780 gttttttttg aagaagaaaa atatgaagaa tgcatacagt tatgtttgaa ggctgtagat 840 gttggaagac aaaatcgtgc tccttatgct ctaattgcta aaccattagc aagaattggt 900 actacctatc aaaaaatgaa agattattcg aatgctgtta agtattttga aatgtcatta 960 acagaagctc ataatgaaga agtcaagaaa aagttaacaa atcttaaaaa gattattaaa 1020 gaagaagaag aaaaaaaata tcgtgatcca gttaaagctg aagaagccag agaactagga 1080 aatcagttat ttaaaaaagg tgattatcct ggagccctta aagcttacag tgaatcagtg 1140 aaacgcaacc ctgaagatgc tagagtattt agcaatcgcg ctgcttgtta cactaaactg 1200 gctgagtttg gacttgcgct gaaagatgta gaaacttgtt tagttctaga tccaaagttc 1260 attaaagcat accttcgcaa aggcaatatt gcattgctaa tgaaggaaac agctaaagcc 1320 agagaggcat acgagaaggc tttagaacta gatgagaatt gccaggaagc taaagatggc 1380 ataatatctg ttatgcgtca aaacgcaaca cttacgcctg aagaacggcg aaagcaagca 1440 atggaagatc ctgaagtaca aaatattcta aaagatcctg caatgcgaat gattttagag 1500 cagatgcagg agaatcctca agcagctcaa gagcatttac agaatccttt gattcgtgaa 1560 aagatatcca aacttctcga gtctggaatt atacaaatgc gttaattatt tctctttttt 1620 tctcctgttt cctattaaat tgtatctact acttaatatt tcaattttta cgtgattata 1680 agaattaaat aattatgtac ata 1703 <210> 30 <211> 1325 <212> DNA <213> Hydra magnipapillata <400> 30 atggctcacc actcgcttgt aaatgtaaaa agtatatgcc cattcctaca aattgtgttg 60 ctatctgtca tactatcaat gtttatttgt gaagatccat ctgttattag aaaccttact 120 gctttagcaa tatctaaaga aaacattcaa ttgaaatgga atcatccaga aatttccaat 180 ggaatcatta ccaattacat tgttgaatat ggagagtttc attcatctaa cattatttca 240 ctttcttttt tagtttttca aagtcatttt gctatgcata catttgctgt tcaagttaaa 300 caactaaaaa attatcagaa atataaattt agggtaaatg cattaactaa tcatgggtta 360 aaccctggtg aatggagtga atggatagat gaaacaactt ttgagggaaa tccatctgtt 420 gttagaaatt ttactgcttc agcaatatct aaaaaaagta tcgcattgaa atggcatcgt 480 tcggaatttc ctaatggaat cattaccaat tacactgttg aatatggaga gtttcattca 540 tctaacatta cttcactttc ttttttagtt tttaagaacc attctgatac atgtactgtt 600 caacttgaac aactaaaaaa ctatcaaaaa tatagattta gggtaaaagc tctcaccaat 660 catgggttaa atcatggtga atggactgaa tgggtaaatg aaacaacttt tgaaggaatc 720 ccaagtgttc cagaagatct tattggttat gctagctcta ctagaagtat tacattaaaa 780 tggacaaaac caaggcaact aaatggcatt gttcgaagtt acactcttgt ttatagtggt 840 tttaaggctt atagtttatc ttttaaacac actggagaaa tagttgtgta ctctactaaa 900 gtaacaattt catcacttta tcctggtaca aattacacaa tttgtttaaa tgcagaaact 960 attgttaaag gtccatcatc catgattaat attacattgc cctatgatga gccacttgct 1020 cctttattga aacttgaaaa tcagaattca gaacaaagaa ccattgtttt acattcagta 1080 ccgagtttta ctgggccaat cagccattat gaatttgaca ttcaagaaaa ttccaataac 1140 tgtttaaata acagtaacac aacaatcaag tttctatcta cacttgaaca gaatcagctt 1200 gtaattacta aaagtgaaga aaatattaca ttccagtcca cctacattca attaagtttg 1260 ataggaaagt tttgtatatg ttatcgagct gtgattaacg acagtaataa aaatagagtt 1320 tcaag 1325 <210> 31 <211> 846 <212> DNA <213> Hydra magnipapillata <400> 31 atgaaactga acctaatgtt atacaatggg cagctaccat tcaaaggaga tgataggttt 60 caacataaat attgtgtttg caaaagaaaa gtaccaaatg gaataaaacc ttatagagaa 120 ataaatgcat taagcgacca agctaaaaag gcaattgccc agaattacac tgtgacattt 180 actggtgaag atggagtcag tagtgtagta gagttcaaaa aagatcacaa aacagatttt 240 tttcaaatag gacgttcaac agaaactatt attgattata tagttataga tacaatacca 300 ggagagaaga ataatattaa agcagcctta aagcaaagta caatatcaag atatgcttgt 360 cgattggttg ttgatcgtag tccaccctat catactcgat tattttctgg gggatttaac 420 tcgaaaaatc gaattgttct taaaaacatt gacctcaccg atccttatgt ctatacaact 480 tgtggtcatg tgcatggtta tcataaatgg ggaatgaaac ctggtgttac ttctgatact 540 gaagattatt atgacaaaag aatatgtaca ttttgtgaac aggaaggcta ttttgtatca 600 ttaaaatttg gtatggaacc agcattttat attgacaatg gtcctgctac tcattgcttt 660 gttccttgtg gtcatgtaac tacagaagct actgtaaaat attggtcgca agtaccactt 720 cctcatggta aggatcattt tagctcttta tgtccgtttt gctcggttcc tcttgatgga 780 agagaaggtt atgtccgctt gatatttcaa actttagttg aacctgtcag agaagtactt 840 TTatag 846 <210> 32 <211> 705 <212> DNA <213> Hydra magnipapillata <400> 32 ccttaaaatc tacaatacaa tacatttaaa actttttaca aaagaaaagt aaagatttag 60 atgtggctta taaattgttt tgcaacaatt tgttgcgtgg gatacagtct ttcgttacga 120 atagaagtaa aaacagatga tttcaattgc agcgaaaaaa ttgttcctga accatgcaac 180 tggaggtact tgacatattt tccaaatcaa tttcgaactg gatcatttga aaatgttgta 240 gtttcagttt ttggcttccc atatacagtt gctgtaaata tcaccctaaa agatagcagt 300 aacgaaaaaa tatacttatc ccaaatattt aatgtgcaac cagaaatttt gaatactttt 360 ccattgtata ttgatcatga tgcaccctca agtttaaaac ttaccttaga gggtcaaggt 420 gtgattagaa atcaagtttg ggtgtttaaa aatgaaagta actatataag tgtatcaaag 480 cttggattaa atgtattgat tcaaacagat aaaccagtat acaagcctgg tcaaacaata 540 aagtgtcgtg ttgtagtggt taatactgag ttgaaacctt acaaaggtaa attgaaggtt 600 gtaatagaaa atccatctgg aactcgtatg cagcaatgga atgatgtagt acttgatgat 660 gtgttacaag gtagtggtgc aacaacaact gagggtttgg tttaa 705 <210> 33 <211> 1821 <212> DNA <213> Hydra magnipapillata <400> 33 gtctacttat taaccaaaac aaagttacac tttttgccat gttttgaaca ccacaagcat 60 ctttaaatgc attacatgct gttgtataaa aattatatgt aaattatttt gggaaaactt 120 tattaattaa gttgttaaga aatattttag taatgtggac cgaaaaatca agttgttata 180 gaattttttc atgtctctct gtccttttta tgacttttga tacgaatggt tttaaaggtg 240 ttgatggtga tacgtcagca agtgttgatc accatcttga aatgggaaaa aaaatgttag 300 cagctggtca attaacagaa gctttatctc attaccattc agctattgag ggtgatccaa 360 aaaattatat gacatacttt aagcgtgcag cagttctttt ggctttggga agatcaaaat 420 cagcccttcc tgatttggac aaaacacttg aattgagagc agattttttg agtgctcgct 480 tacaaagagc aagcattcta ttgaaacatg gaaagtttga agaagctaaa gctgactatc 540 ttcatgtgag tagacaagat tcttctaatg cagaggctac tcagggtcta tctattattg 600 gtccagttca tgaaaaagta aagacggcag aggaaatgtt tgaacaccac cattggtata 660 tggttataag tcatctcgaa aatcctattg aggtatgccc ttgggatgtt catttaagag 720 aaatgcgagc gaaaagttat gaacaaatag gagattactt aaaagctatt caagattatc 780 gaattacaac aaaattaaaa agtgatgata cagaaggcca tctgaaagtt gcagaactct 840 attacatact cggggaaggc gaagattctt taaaagaaag cagagaatgt cttaaacttg 900 accaagacca caaaaaatgt caccagcatt ataagaaagt taaaaaacta gtcaagttct 960 ttcaagacgc ccaaaaatat atggatgaag gaaattatga cgacgcattg ttaaagttaa 1020 atgcagccct taaaatggag agctcaaata ataagtttgt tgttagtgcc aaagaaaaaa 1080 tgtgccattc atatgttaag attaaaaact caaaggaagc ttttcgagtt tgcactgaaa 1140 cattagaaat tgaaccacag aatgctaatg caatgtgtga cagagcagaa gcttatttat 1200 tagaggacaa acttgatgag gccttaaaag attttcaaag tgcaaaagaa attaacaatg 1260 atctaaatag agcaaaagaa ggaatagata gagtaaacaa acttattaaa caatctaaaa 1320 aaagagatta ctacaaaata ttaggcgtta gcagatccgc aagtaaagca gaaattctta 1380 gaaaattccg aaaattagca tctaagtggc atccagataa atatgacggt cctgacaaag 1440 aaaaagcatc aaaaaaattt attgacttgg ctcatgctaa agaagtgtta tctgatccag 1500 agaaacgtgc aaagtatgac caaggtgagg atcctttaga tcctgaagct caacaaggtg 1560 gtgggcataa tccttttagc ggatttcata acggattcgg tgaaggcttt aattttaaat 1620 ttcactttaa ctaaatggtg cgtcctcatg gtctgttgga gaaaagctat ttcaatttcg 1680 tacacagagt accttctact cctagtagaa agaagaaagc ttttattgct aaaaaaaaca 1740 ttcgaggtca tacatacttc aatatattta acaatgttta aaaccatata tatagataga 1800 aaaataaatt gtttgctatt a 1821 <210> 34 <211> 798 <212> DNA <213> Hydra magnipapillata <400> 34 atgatcgaag aggaatccaa gttgatcatt tctagagcaa gtgtgtccac tgattataat 60 tcagatgatg attcttaccc gactttaata atgaataaac attctagcag ttttaacgaa 120 gaacaccgaa taaacaatga actttttaac aacaaaccaa gtcattccta cataagtttg 180 atagctaacg ccattttagc ttcacccgac aaacgcttag tattaagtga catttataaa 240 tatgttcttg agcgttacga ttactttaaa aaaaaaggct ctggttggag aaacagtatt 300 cgacataacc tgagtttaaa tgattgtttt ataaaggcag gccgaagccc aaatggaaaa 360 ggacattact gggctataaa cacagcaaac tacgaagatt ttgctagagg ggactttaga 420 aggcgtcgcg ttcagagaag agtgcgtcgg ggtgtgtcgt cacccacata tcctccgtac 480 gcgattaact attacccatt tcagcttcat tatccacaga ataaaggatt tactgataac 540 tctggataca tcttggaaaa agacatttta tgctcaatcg aaaaatattc atttgaaaaa 600 aaggaagagc ctagggaatt tttccctcgg gaattggctg aagcaaaaag tcaaacctct 660 agtgctaaaa ctagtcattc tgtaaaatca aatcgaccat ttgacattga aaacctattg 720 aaagacgata aaattacaaa gcgtaaatat agcatcgaac catcgagaat ttattttgaa 780 cgcccctatt tatattga 798 <210> 35 <211> 1000 <212> DNA <213> Hydra magnipapillata <400> 35 aacagagttg cattatccgt aagcacattt ccagactgat cattaattgt attctccgag 60 caattttatg agcaccagtt acaaaatcag attggctaag taaatttatt tctacagtat 120 tataaagcct aatgagttgg tcatacagcc ctacgccacc gtttcccctt tctcatcatg 180 tctcaaataa ttattctgca tattttccat ataactacaa tggttttcca agaaaacagc 240 gtcgcgaaag aacgaccttc actaaatttc agttagaaat tcttgacaat ttattcaaag 300 aaacaaaata tcccgatgta ttcatgcgag aagatgttgc acgaagaata tctttaccag 360 aatcaagagt tcaggtgtgg tttaaaaatc gacgtgctaa gcatcgccaa aaaagtaaaa 420 agaaaccatt tcaatcagat aaagattgcg atttagaaaa gttatgctcg tctacggttg 480 ttaacaacaa acaatctcgc aatacagttt ctatattgcc ggcaaaatct gtggaggctg 540 tgtttaaagc aaataactca gaatcacaaa gttcctttga tgtaaatcaa caagcaataa 600 gacaaaactc ttcatttaac tcttctaacc aacaaatttc ttcagcagca ttagaaaaat 660 ctcaaacgtt tccttgttat ttaccgacat ctttatcatc ttatagaccg tttttaattg 720 aagctcattc ttcaacatac tcctctgctc aggcgcgtag tttttactca aactacgcgc 780 aagcgccagt tgaataccaa tcttgtgtag ttaatttcaa taactctcaa gcagtcagta 840 cgcaatggac aaattatgga tcatctattt gattttttat gtttagactg ttgaaatact 900 tttgcgtaaa gcgcattaaa aattaagtaa tgaccgccta gttatactaa ttaaatattt 960 aaattatgta tctaaccaag agctataaaa caaaacaacc 1000 <210> 36 <211> 429 <212> DNA <213> Hydra magnipapillata <400> 36 atgtcaaaaa taggcaaacc aatgtggtac gtgtttaatg caatactgtt tctaaatatt 60 tttgtgattt tttcaatcaa ctcactacca cttcgcgatg atgaagacac tgataacgga 120 atctatggcg acactactaa aatagaaaat gaacgacata gctataaaat cactggttat 180 aagaaatcca aaaatcaagc agaattaaaa accaattcta ttaaccacta catacccttc 240 tttttaccac agccgaaaac cgttcacgat gtaaaatctc actctgtttc aacagcatcc 300 gacgaagatg gatcaagaaa atcaatcaaa ttttcgcgag aaaacaggta tggacaaaaa 360 aaatctaaca ctaatccatt tatctttaaa ggacataaac atggtgacca aaaccgtcaa 420 aagtcttaa 429 <210> 37 <211> 578 <212> DNA <213> Hydra magnipapillata <400> 37 atatatatat atatatatat atacatatat atcaaacata cacacataca aaaataaaat 60 gttgtcttta acagttgcaa cgcttcttct tataacatct attgtgatgg ccatgcctaa 120 ccgtgatgct actgactcaa atgaaagcga cattttaaat attctggacg agtacattgt 180 aaaggttgct gagatgactg cgaacgaagc taaaattctt aacgatgtta gaaattacta 240 caatgataga agctcaaaaa gcttaggaga gtttccccaa agctttttac caagaggagg 300 caaaagagat gcacgccctc gagccggaaa ataaaaatgt aatttgatac aaaaaaaggg 360 caaagaagga atttattcga acaatcttta ttattggtat atgaaataat ttgtttattt 420 gaatattaaa aaaatagttc tatgtatata taaaataaat tagtcatttg gcattttggt 480 agtttttact tcttggtata ttttatttta tttaaaaatt ttaaattatt tgtaacaact 540 ttgggaaaaa accggaaaaa attgcgtgaa aaatttaa 578 <210> 38 <211> 1763 <212> DNA <213> Hydra magnipapillata <400> 38 gttgtgaaga agcatcacgt attcttttaa aacatggagc accagcagga gtttatgatg 60 attcaggaac gtcatgctta tcacacttga ttgcaaaaat gcctaatgta gctgtcgagg 120 ctttagatca atttcaacat gttgataagg caaaaagaac aactaagtat tatttaagtt 180 acttagaaac caaaaaatgg aaaaatagta aaaaactgtt aaaaaaaagt gtaagagtgg 240 ttttggtcag agaaccttta gagaacattg taaaaaatca agattcatca ttaattatgc 300 atccagttat tcaacggcta attaaaaaaa aaaaacagtt atatggtcag catagcttta 360 tttttgtcct tttgatcaac ctcttgttta caacaatatg gactgctctg acttttacac 420 ttcctcagta taaatttggt atttcatctg atttaacaac tgtagctcct gaaactatct 480 tccccaacat caccgtgttg gctgatttaa catttactaa atcatatgat tcaaccttat 540 cactggattc aacaatatca ctggattcaa tcaatacaac tgcttcaacc acacaaagca 600 tttcacaagt tgaattttat actccttatg ataaacagag ctggagattt gttttagagt 660 tcattggatt gcttttagct atctactttt tcattcagac aaaagttcaa tttaaaatgg 720 caacagaaag ttatgcaggt gttaaggttt ctcgtttgca agagttaagg cgagatttaa 780 atttttgcca tcctcggtgg ccacaagaac gaaaactaat tgaagaagaa attcgtaata 840 ttagtgcaga aacaatggga tcattgtata atgcatggat tgtttttgat atgctttgtt 900 acataggact tgtattttta atggttacta gaattatttt gatttgttct aaagaagtag 960 ataatactta cgaaacaaca ctacgagctc attattatgc atttcctgtt gttcttttca 1020 taatatggat aagatttatg tcttctttta gacctttttt tgctattggt ccatttattg 1080 ctatgtttgg gagtgttgct gaagaaacag tgaaatttac atttttattt atggagtttt 1140 taatacctta tgcatgcatg gtatggatta tatttggtgg acctcagtgg gatgataata 1200 gttatgctaa ttttgatgat gtaatgtttc aacttcttcg aataacaaat agagacagtt 1260 tttcttacac aaatcttttt acgcataatg gaacagtaaa tacatcagaa aaggtggttg 1320 cccagttaat ctgtggatcg ttctatgctt ttatgtcaat tacatgcatt agtctttata 1380 tcggaattct ctcacagact tttacaagag tgttttcaaa tgcagcagcc acagcttaca 1440 tgttgcaagc agaagccttg attattgctg aaaaaaaatt aagcaaaaaa aaaaaaaagg 1500 aggttcaaaa tgcaatagca aattactgtt ctcctgagat tgtatttgaa attgaagatg 1560 aagctgaagg cgcttcccaa gtattggcta tgcttgaaaa cattagtagg caaatagagt 1620 ttcttcaaaa aggtcaggca gagttgaaac aaaaatcaga ttatatagaa gaggaggcat 1680 cgtcattgct ttacgaaaaa gataaaatta tagatgattt aagtgttagg attcaggact 1740 taatgacatc ctccgatact tga 1763 <210> 39 <211> 145 <212> DNA <213> Hydra magnipapillata <400> 39 ttcgatgaag atggaaatgg atctataagt tcagatgaac ttcgcgatat catgcttaaa 60 tttggagagg atttaaccga agaagaaatt gctgaaatga ttgttgaagc ggattttaat 120 ggagatggaa atattgatta tcaag 145 <210> 40 <211> 1322 <212> DNA <213> Hydra magnipapillata <400> 40 tagccatgtt tgtgcgaact ggaaccgtca aaatttcaag gtctctttct aatgtctctc 60 aaaattctcc tgaaaaaaag ttgtttaatc agcaaaaaaa aaaaaaagaa agttttcaat 120 gtagcttttt agcgcatcat attaatgcta tccgcattgg aggtttagtt cttcatcaac 180 aaagagtaaa aatatgtgtt cgaggtacac ttttcgaaac atttgaaagt actctcggaa 240 atttcccaaa tacattactt ggaaatattt ccaaaagaaa cacttacttt gacgaattac 300 gcggtgctta cgtctttgat agatgtgtta attcattcga tgccgtatta ttttactatc 360 aatcaaaagg aactcttgct aaaccacctt tagtaacaag agaacagttt tatgaggaac 420 tcaattttta tgagattgat ggagtaatag atgaacgaca catggaagat ttgtattttt 480 taaaacaaaa cgacaaaaaa aatggggatc tacctaaagg gtgttttcgc tcaactgtat 540 ggaagtttgt aaacttcttc aatgataact tcctccaaac agcttacaca tatgttaatt 600 tactaataac tttgctttct attactgcct tctgtttaga aactgaacca tcattgcagt 660 ttgccaagat ttggatttat atagaaatag gaacaggaat cttttttggt attgaattta 720 tcttcttcgt ttactcgtgt ccaaacatct tccagttaag acgttcttta aatgcactga 780 tcgacttagc ttcagttatt gttaacgtgg tttatatttt tcttttacttt ttggttggta 840 ataaggtgac tgtgctgata cgatttttta gggtaatacg cgttttcaag ctaacaaagt 900 ttagtatggc gttgaggctg tttatttata cgctatacaa atgcagtaat tttcttcaga 960 ttatattagt gactgggggg actttgagct ttgcatttgc tgcttttatt tttatattag 1020 aaaatcagtt taacaaatat aaggaagaaa gcgctttcag aagtattttt gatgctatgt 1080 ggtatgctgt tatttcagca actggtgttg gttatggaga tatacatcca accactgctt 1140 taggaaaagt ttgcggtgca tttttattag ttacaggcat tttgctattt tgtctacccc 1200 agcctgtctt agtgaaccaa tttatatcgg tttattactt accagaaata atgtgcaaca 1260 aagaaacttt aagaaaaaat gccatcatga agatgaggca aacaatgctt ggagaggtgt 1320 aa 1322 <210> 41 <211> 1118 <212> DNA <213> Hydra magnipapillata <400> 41 agcattcagg ataaattatt ttataaaatt tctaaaatta tttgaagaaa ggaagtttaa 60 gaagaaataa tcaaaaccaa aagaaacgtt tctaaacgtt ttaccaaaaa tgaattttga 120 agaactggca aaaatagtag tcgaggctgt acaagaaaat aataatcagc caataaatac 180 aaataagatc aaaactcctc gtgaactaag aaacaacaaa acaaaagagt acgtttcaga 240 aatgattgat aattcttctt ttcatggaat tagttatata gctggtaaag aaaaccattt 300 tattcgacga acgatatggc tactcataac aatgacagct tttggttacg cagcacaaaa 360 agtgtatgaa agcacagtta actatttttc ttttccaatt agtactactc aaatgagaat 420 atacgttaat gaaattgatt tcccggctgt atctttctgc aatttcaacg aatttcggtt 480 aagcaaaatg gacggaacaa aagttgacca agctatttta aatccaaaat tacaaggact 540 ggtaacagct gaagaataca gaaacgtgac ctttggagct atgtttgact taaaagaaat 600 gcttgttgac tgtgagttta acggtatacc ttgttctgat gaaaatttta ccatgtttag 660 ttggatgcag ggagaacgtt gtttcacatt caactcagga aaatttcctc ataaactttt 720 aaagattggt ggtgcaggaa tgaaaagaag cttgaaaatc acaataaata ttatccatta 780 cgaatactat aaagacgaaa tggatgcggc tattcatatg atagtacatg gacagcaaga 840 cactccactc aaaatgcgcg gccctacatt atcacctggt tttacaacat atatccaatt 900 agaaaaaaag atgattatta acttggaagc tccttataaa acaaaatgtg gttctttaaa 960 gttaaaatat tttgatagtt attcattaga cacatgctgg cttgaacaac ttacagatca 1020 tgttcatagt gtttgtaaat gcaaagactt ttttatgcca ggtgatattc caatatgttc 1080 attaaatgat gcaatgagct gtatgtggcc agaatggg 1118 <210> 42 <211> 3205 <212> DNA <213> Hydra magnipapillata <400> 42 aaaatgagtt ctatagcaag atttgcaaat atagccaaag tttcgagtag acatgttgat 60 gatgtttcga aaaaagaatc cgaaccgttg attgaaaaaa aagaagaaga aattgatact 120 ttgctaaaaa ataaggatgc catctttaat aatgaaacaa aacatgaaaa taaaaaatcc 180 ttcaaaaggt ggtcgctgtt aaaagaacat ccaaactatt cacagattat aaatgaggtt 240 gttgaggtgc ccgactttca agagactgac actaacaaag agctcatttc tcagggaacc 300 gacactaaag aagttccgaa ttttcaagag acttctaata aagaaataac caattttcaa 360 ggaactgaat ctaaaaatga tgaggcatca gacaaaatag ttttacctgc tgataatcaa 420 tctgtcgatt cagaggtaac gctaaattta agtggaaata caaaatgggc agcgttaaca 480 aagaaaaatg ctaaaaaaat tattttggaa tctgacaaac ttaaacaacc cgtattgagt 540 ttaagtagtg actcgacttc acaaactgat aataaaattt taaataataa aaattcatta 600 ggaaaaagta aatggaatgc tgtaagggag cgtactaact ccataaaaaa tactaaagat 660 gatgtgaatt caaacgaaaa accgtcttta ccaaaactgt tacaagttgt taacactgcc 720 acagcaacta aaacagctgc atcaaagttt ttagaagcat ctcaaatgag agttaatctg 780 aaagcaagaa tgcgtaaaat agttcaagaa aggttgacag taaaacaaat atttaaacga 840 tacgttgaaa gttcaacgct tcatggtttt tgttacgttt gtggcgatac ctttttagta 900 agaagagtat tatgggctct tttaatgata ctaggagcaa tatattttat tattaaactt 960 agatatggaa ttgaggaata tttaaactat ccatttagta cattgtcaac tgttgattac 1020 gttcacgaat tgttttttcc agcaatgagc ctttgcgcaa caaacagtta ccttgcttca 1080 atggtttcta aaaatcagtt aaagttactt tacgatgaag gaagactacc tttagataac 1140 aatcaaacta acccctctta caatatgtca ggaaacgaat tagtaaaagc aattcaagaa 1200 agctcattat caatagagtc aatgttagca ttttgtgatt ggattcaaca agatactaac 1260 aaccctgata taccgccaaa tccctgtggt cctcaaaact ttaccaccta tctaaactac 1320 aaaggtgagc aatgttacac gcttaactct ggattagaag gacataaact acttaaagtt 1380 gatactgtcg gcttaacata tggttacgag ctaatttttg atttacaaac aaacaaagta 1440 attaaaaaca atcagttttc aggtatgaga gtagtaatac ataaccaaga tgttccacct 1500 caattagctg acgggtttat aattccccct ggctttaaaa catttgttaa aatgggaacc 1560 gtacagtcta aaagtcttcc tccaccttac tcaacagaat gcggaacaaa aaaactgaag 1620 tactacaata catacagtca gagattttgc cttcttgaaa ctcttacaga ttttactgga 1680 aagttatgtg gatgcagaga cgtttttatg ccaggaaaaa cgccttttga gaaaagaagg 1740 cgaatagact ggattaaagc tattaatcgt aaggattggg acagttgtga aagtgaaaag 1800 attagtaaag aacgagtatg cagtgcacat tttgtatcag ggaaacgttc tgatgaccca 1860 tctgacgttg actggagtcc atctgtattt aatcaccttg gatatgctac gatttctact 1920 ggggcagtta gttttttatc tcctggctgg ggtggtcgtg tttcagacaa acaaataaca 1980 tttgagtcaa acttttgtca gaagctttat cctggtgact atgttcttgc tgacagagga 2040 ttcaatatta aagatgaact aaatgctgtt ggggcaacgt taaaagttcc agcatttacg 2100 aaaggtagac aacaattatc tggatgggaa gtggatacat ctagacagat atctaatgtt 2160 tgcatacatg tagaaagagt gattgataaa tttatacttg ttggatcata tattgatgtg 2220 agagtttcag gaagtgttgt ctcttctgtc tcagatgaag tttcagtagc actagagtca 2280 tattcaacag acataaattc ttctaaaagt cttcctccac cttactcaac agaatgcgga 2340 acaaaaaaac tgaagtacta caatacatac agtcagagat tttgccttct tgaaactctt 2400 acagatttta ctggaaagtt atgtggatgc agagacgttt ttatgccaga aaatggactt 2460 ccattctgtt cattgaaaga gttatattct tgtatgtatc cagcaaaaga atcattttct 2520 gaatttacca tgcgtaaaga atgccctgca gattgcgaag agcgtacata tccgtatgag 2580 ctatcagaag ctagatttat acataaccct ccaataggtt tatcaattca aggtttagca 2640 aatttgcaac acaaaaaaca tttagaggaa gaattatact tatcaaagtt agctcaaagc 2700 atgacatccg aggaacttga tgcatatgtc gaagacaaca ttgtcagcgt gatattcttt 2760 tttggtgaca ctcgaataga ttataatgaa caagaagcta caaatgattt ttttcagttc 2820 ctaggtaaca tgggtggcga gttcggacta atgttgggtg ctagtttgtt aacatttgta 2880 gaatttgttg atttatttat ctttctgata tatcatcaaa tgctcagatt acacaccctt 2940 aaaaaagttc ctgatatttt tggaaggaaa agaagtcgaa ttaatgagta cattaaaaaa 3000 cgcgagaaaa cacgcgtgta acgtttgttt ttaatgaaat atctttcgag tttgatttgg 3060 aactttcaac agcaatgcat atgttagttg actttcagct ggatactttg gaaaactttt 3120 gtaaacgttt acaaaacatg aagtctactt ttaaaactgt atttaaaaaa cttattaatt 3180 aatttaataa atcattaaag taatt 3205 <210> 43 <211> 4781 <212> DNA <213> Hydra magnipapillata <400> 43 ctattgctaa aattgcagcc aagaccttga ttgatacttt ggaagaaaat gattatttca 60 acatgatgac tgtatcaaag actgaaagaa tgaaactggc aattaataat attgatgaac 120 caaaagatat tttagatatt tcaaaagcaa ttagccgtgc atttgattca cttaaagata 180 atacaacata tacagctggt tgtaacaaag ttattatgat aataagtgat ggcattgagg 240 gagattacag ctctacagca ggaaatgttt ttgataaaat gaatgcagac aaatcagtac 300 gagttttttc ttatcttgtt ggaagagtga aaaatccaga tgatcgtgct ctcaaagaaa 360 tgtcatgcaa caacaggggt tatttttata aaattgaaac tataggaaat gtttgggatg 420 ttgttgtaga gtatttaaaa gttttaagtc gaccattagc agcaaatagt gtcaagataa 480 aaccaaaaat atctccaatt tacttagaca gttcaggagc aggtatggtg ttaaccatgt 540 caatgggagt cttcaacaaa aataatctct ctggtgtagt tggagttgat atgcttataa 600 attcgttgaa aaaaaaggtt tcatttaaag agttgggtta tctcagtcat gctattatta 660 ttaacataa tgggtttata atacttcacc ctaagtttcg agatcaaact gggtatcttt 720 ctgcatcagc taatgtttac tttgaagatt tggaatatag tgttgataaa aatgactcga 780 ttgcattaaa aataaaaatg ttaaatagaa atagtggcaa catgagtttt tcatcatatt 840 ggttgtatga tgataataat agaattgcgt tacacaactt cacatatttt ttttatccaa 900 tcaataacac aatactgagt ttggctttag caataagcga ttatgaaatc aactacatgg 960 ctgttaacca acaaaaagta acagtcgata taatacaaaa tggattaaat gctttaaatg 1020 aaatagttga tttcagctgc agtcatcatt attttactta cgtgcaaata gctgctttga 1080 tgttttgcaa catttgtaat gaaacaagtt atccaacagc tagccaggtt tattcttgtt 1140 tatcatcaaa aagtttggat agagtgaaaa aagaatgtaa ccatgattta tttaataact 1200 tgataactac agctggcata gcacataaaa gaattctaga gagtttgaac aaatgttcag 1260 ataaaaaaaa tgatgaaaat ttttttagat cactttatgt aggaactaat ggtggttaca 1320 ctagagacag cttaaaggaa attgtgttgg aatttaatga tccaaaatca ataaaatatg 1380 attatttaaa tggagaaata attgccttag acaacaaacc attatcactt gttgaacaag 1440 ttggcttcaa caagcttatg gaaaaggcag cacataaaga ggctgcgcga ttggaggaag 1500 ttagtaactc tccttctata gttaaaataa atgaatctca gagtcctata acggataccg 1560 ataactcaaa aaaagcatca acttttgaag caatcttaaa gttaatgcgc actgcatgtg 1620 aaatggcatt gcatcctgct atgccacata gtcattttga gatacttata aaggttcaac 1680 gtgataatgt catagaattt attcattgca ttgccaaggt aataaaagtg aaagtagcga 1740 ctattattgc tagcaaatct tttatgactt tgctatctga tggtagccaa gcgcggaaaa 1800 cacgcagtga aaaaaaaacc atattagtta gagttgaaaa tgccggtatc ccgtgttatt 1860 tttttgttgc attgtgtaat atgtcggatt ttggtggagt tgatgctgac tccttgaaat 1920 tggcattgga tagtgtattc attgaaaaca ttatagctaa atgcaatatt ttattatcca 1980 ttacgaatta tcgtacaaag ctggtatcag caacagctga tggagcgaat gttaatctgg 2040 gtatctataa tggtgcctta actcaattaa aaaaaacaaa accatggtta gttaacatac 2100 actgtgttaa tcacagatta gagcttgcta taaaagacgt tataaagcaa tttaaagact 2160 ttacggattg cgacaatttt tatacaactc tattttatct tttaaaaagt cagtaccatg 2220 gaacagtgtt gtttattgct ggaaattgcc atgtatggac agatgatcag cgtaaagata 2280 tattaaaaga agctgtttac aaatacttgg gaaaacgtaa agttgttaaa tttcctattc 2340 aaccaaaaat attaaacaaa gaaaatacgt cgaaaactga taatgctgaa gttcatacaa 2400 atattaggca agcggctata ccagttgaaa gtgaaataag ttcttccgaa ccatcaggtt 2460 cagataagca atatcaagga tccagcaaca gtgatgactg ttccaacgat ggtagcacat 2520 cagattcaga tgaagaacac agtatctata tagtttataa taggtttttt tcaataaacc 2580 aaactatacc tcctgaacca gattcactac ggacatcgat ttttgaacga gcacaagcag 2640 ttccatgtag caaacttgtt gtatcagctc cttttaacac aactttaaat aatggtcctc 2700 tttatataac agttgaagct tcatcttgga ttcaacatgg taaaagtaaa actttggtag 2760 cagttactgg tactgaaatg cattctggtt tcattaaaca aatgtttgat aatgcaactg 2820 aaaatcttaa ctgtaagaac aacgactctg ttgtttgcgc catagttgat caacacggct 2880 atattgtcgt cagtaacctt ggtgataaag caataggaag cttttttgga aaagaccatg 2940 gagctttgat ggatcactta tctagtccta atatttctat atttcgaaaa attactttag 3000 atgacacaca ggctgaatgt ccggttccac aaatatacga ctctgcctct aattttttgt 3060 taaaccctat acaactaatg cttagtttaa gtacatggct tattggttcc tgttggagct 3120 tgcttttaca tataacagta ttaggtacct ctttttttcg aaagtattca gcaaatgctg 3180 aaacaacaat tgatccaata aatatatatt ttccgcgatt atgccatata atgcctgctc 3240 aatatatgcc aaaaacgttt acactttcaa accttttaat ggaaaaccca aacaagataa 3300 aatgtcattt taatgatcaa ttgaaaaacg aatttgattt cctcatgtca gagagtgaaa 3360 ccattgtttt ttgtaatatt tgcagaggag aattttgtat tgcagttgga gggagaagtg 3420 ctataacgaa acacttaaac accaacaaac ataagaagtc attagatgct tcagcttcca 3480 ctagtaaagt aacagatttt ttcaaatcat ctaattattc tgagggtgaa aaacagttag 3540 ctgtaatgga ggggacattt gcatatcata ccataattca taatcaaagt tttagaagta 3600 tggattgtac aaaatctatt gatggtgaaa ctgctgatat tttgtcacaa cacctattca 3660 gggttataaa tgaaaatgat ttgaaatcca aaattgttgc actaacaact gataatacgg 3720 attttggtgg gcgaaaacgg aaaggagtaa ataatgtcca tgtaaaatta caggacttac 3780 ttcaaaaaaa aatactagga attggttgca atgcacatat tttgtcaaat gcaattaaca 3840 cagcatcatg tgcactgcca attgatgtag aagttataat aaccacagtt tatctgtatt 3900 ttagtcgttt tacagttcgt gttgctactt taaaacaatt ttgcgaagaa gcaaatgtcg 3960 aatacaaaaa gcttttagga tattcgaaag taagatggct tgctttaaca cctgctattg 4020 agcgtgtttt aaaattattt gaacccctcc gatcatattt tttaagttta gaaaaatgtc 4080 caaaaattct tgaattattt ttcaataatg aattatctga gatatttatg tattttgtac 4140 acaatcaggc aactttattt cacaaaacga ttcaaaagat tgaaagcgat aatatatctg 4200 ctgcagaggt tagccttatt ttaaacaatg ttatccttca atataaatca caatttaaag 4260 actggtctga gacaaatatc acagcatata acagttttca atggtgtttt ttgagttcac 4320 atgttaattg ggcagatatt gaaaagtgtg ttgagttttt attaacagaa attcctgata 4380 ttaaaattaa tgacaatagt ctgtatgatg aaataagaag agtaaattta tatttaagtt 4440 cagataaatt agaacaatgg aaaaataaaa atgttgaaat tgataaaaga tgtgtggaaa 4500 tatttgacca atttaaaaat aaacacattc catatgaaaa tttatctatt cttgttgagt 4560 ttgggttgag ctgtcctggt actaatgcag cagttgaaag agttttttca attggtaatg 4620 atttctggac cagtgaaaaa tcgagattaa atattgatac tctagcagca gcactcactg 4680 taaaagttaa tatgaaacac atatcttgct ctgaaatttc aagcatattg ttagaaaacg 4740 aaatattggc aaaaaatatc cactcagctg aaaaatacta a 4781 <210> 44 <211> 755 <212> DNA <213> Hydra magnipapillata <400> 44 ggacatttga aaaaaagttt gaaatgagtt cgtttttaat ggaaaacata ctcaaagaag 60 aattcaatac aaaaaaattc agcttaaaaa atctccaaca aaaccaaccg aggttagagt 120 ttcaatgcat ttcacacaat actagtcctg aatttaaaac gaaaactcaa gaaagatatg 180 aaagaaatat tttattagac gaatacagtt gtttttcgca ctcatgtcat ttgtgttacg 240 gacaattaaa ttctgatcca tttaagttaa gttcaaaaat ttcttcggag ctacggcgtc 300 aaagtagtca agattaccct gacctaaact acatggagcg ctcttgtttt tattgtccta 360 cacaacattt cattttgaat aatacaaatt tgaacacgga taaaccattc aaaaagaatt 420 catattcttc acatttctta aaaagaagac atagaacaat tttttcggac gaacaattaa 480 acgtattaga aagattgttc aataaaacgc actaccctga cgttattgtg agagaagaaa 540 tagcaggaat aataaattta acagaagaaa aggttgaggt gtggttcaaa aatcgccgag 600 ccagatggag aaagcaaaag aaagatttgt ctggaattta ttaaaagatt ttttgttgga 660 atataaaaga ttaataaatt ttgtagatag aacccaaatt tttttttttg tgatctcaaa 720 tgtaaaatat ttagttaaaa ataatacaat tactt 755 <210> 45 <211> 1185 <212> DNA <213> Hydra magnipapillata <400> 45 atgactgaga acaaattaca aataccacca gtacgcttaa aatcatgtaa tgaaaaatgg 60 tataatcagc atttaaaatg gaatcctaat gaatttaata atatcagtgt agttaatgtt 120 agtcctgaaa aaatttgggt accagatgtt gttttatata acagtggcat tggtagctct 180 atgtacagtt ttaaaaccaa aatcattatg agatacgacg gattacattc ttggtattca 240 ccagctatta tcagaagcag ctgtaatatt gacacaacat actttccatt tgacgaccag 300 ttatgtattc ttcgatttgg aagttggaca tttgatggtt atcacattga tatgtttcca 360 acaaatacat ctgctgattt aaggtattac ttaaacagta ctgagttcaa gctacttggc 420 gcagatgttg ttagagaagt tgtaaaatac agttgctgcc caacgcctta tccaaacatc 480 gtatttacag tcaatttacg acgtcatcct ggatatttta tgattaacgt aataattcca 540 agtacagtga tgacactatc aggtgttctt acatttggct tgcctcactt aacgggacaa 600 agaataagtc ttgtgataga atcattttta tcacttacat ttttatgcat gatggtgtcg 660 gacagtactc caattaattc agatgtgtca ccgcttatta caaagtcact gatgttgtat 720 atggggttga tttcattagc acttgtcttt aatattattt cgattggctt aaaacgccaa 780 caaccagttc cgaaatggct gcgaatatta gctttcaatt gtataggtcc cttagttgga 840 gttaaccata taattccaaa gtcgccaaga cattacgatg aagaaaacca agaaccgtat 900 agtaaaaaaa atctcctcgt taagtttcac gaaaaatata aaaacattcg aaagagttca 960 aaaaagtacg aaacaaataa actgagttca gatattaata tattagtttc aagatcgttg 1020 caaaaaactg aatcaagaag taacgaagag ttttggaaat gcattgggat aatcgtggat 1080 cggttatgtt taattgtact gggctcttgt tttctcatag tttccataat tttatttata 1140 aaaggttacc gtcatcaaat aaaactcgca gaatctttga aataa 1185 <210> 46 <211> 2139 <212> DNA <213> Hydra magnipapillata <400> 46 agataactaa taagaatgta tcgaggagaa agagctgtcg tgaacgacat gcgaatcttt 60 cgaaaaatta tcgatgaaag atttgcaagt ttaattcgca tggacataga atttagacaa 120 atattagagg atcttgtcat tgaccttcat gaaaatccta agggtatgat tgatcactta 180 gttgaacaca gatactttaa cgaattagtt tatggtatac taagaattat ggatcatcat 240 actccaatgg ctccaaggat tgctggaaac gcagcgtatg tcattggagc tttaattgat 300 tcacccgtcg gaaaagaaaa ggttgttgcg ttacttcaaa atgaagatcg agttgaagat 360 acgcagcatt gtttaccaaa ccttgctgct attattgaaa ctggtgatcc tgaaccaatg 420 acaaatgcat ctggaactgc atcacttatt tttgaaagtg aacaatgttt gaactgggct 480 ttggaacaac catcttgtga gtggtttata tcagtgttag gtcaagcact tgttaaaggt 540 gacatgtggg ttgccagcaa ttgcgcgttg actcttgctc gaatttctat gaacaaaaat 600 gggttggaaa aaattgctag acatcctgat tttgacgaac tagcaatgca gttaattgct 660 tctgtaggtg ttgacgatga aggacgaggc atgaacgcag catttgcatt gggatatctt 720 tgcgaaaatg aggatttaat tgcaaagatt gtggactata aagagttctg ggatctgatg 780 catggtttaa ttcaaatgtt aaggagcctt gatgatggtt gtaaaaaaaa ctcggctttt 840 tgtcttaaag caatttcaca gtggaaaatt ggtcaacaac aaataaatga tgaccctgat 900 atcacttatc ttcttgaaat acttacaaaa cttcttactg caggtgacga agatcttgca 960 aaaatggctg ccaatatttt gtgcaacttg gctcaaataa aaaaagggta tatgctgcaa 1020 aaagatgatg aaaaaattgc tgtgaagcca actttaaaac acgtgttatc tcaaatgtca 1080 ataagtgaaa attacagaga agaagcattt aaagcaatga aagctttaat gctagaaaag 1140 ccagagcctc cagtgcttaa agttcttgat gcacattcaa ttcatgccac ttgggaagtt 1200 atttcatcca aatgcaatgc aaatgttcaa tatgaattgc agtgtgatgg agctaatatt 1260 gtatacttgg gtcctgacac ctcatacaca gttactggtt tgaaggaaaa aactttttat 1320 gagtttacac taagagctat tacagatgaa aatgaagaga gctttacaag cgatcctgtg 1380 actgttcaaa catttcgttc agtttctaca cctcctcaaa atttaagagc aatacaagtt 1440 acagctagcc aggttaagat agtttgggaa aaaccattaa agattattgg caaccttaaa 1500 ggctatcgtg ttaaagaaac tggaagttca attcattatg aacctgtttc tttatactat 1560 attgccagca atttagaagc agacaaagag tacacgtttg aagtttgtgc agtaacacaa 1620 cgaggagatg gagaagtagc aacagttact gttagaacac ttggtcatga ttatcatgca 1680 ccatccaaac ccaggttggt atgtataggt ccaaatgaaa ttgtttgtac atgggaacca 1740 cctgcagcaa aaaatcttag aataaagaat tatgaagtca tatgtaatgg aaaggctatc 1800 tattttggaa cagtaagaaa atgtattgca aacagattaa aagaaaacac agtccacaca 1860 ttttcagtaa ttgcatggac gagtgagggt cgcagagaaa gtcttccagc aacaaaaaaa 1920 actacaggtg gttataagaa aaaagcatta aaaaaacgac cttggaagaa tgagtcagaa 1980 cttcaaaaat ggttgaaaga tgaagaaact gaagaattaa aatcagattc agaagaatca 2040 gataatggtt cagcaccaac agatgcaaat gataacgaga atgaggattc tgaaaatcca 2100 caaaaaacag acagtgaaaa cgaagaagac agctcataa 2139 <210> 47 <211> 4291 <212> DNA <213> Hydra magnipapillata <400> 47 ggaccttttt aacattgatg gtatcttgcg taaatatcct aaccgaggtg ttgatcgtga 60 tatccacaaa tgcaagcaaa aaattgctag atctttagaa ggttttaaaa gtgtttctga 120 tccaggaaat gattttattt atgaaattga acagttagat cattatagag atgaagtttt 180 ttctatgata aagtatgaag atggaaaaat tgataaaaga gaaatcttct taacacctac 240 tgatatttgt tgcaagttta ctatctccaa tgttcggtca tgtttatggc aatgttctta 300 cagtaacatt tgtggtgtta aagttcaaga atattttgaa ggaaaaggaa gagaaataag 360 aatactcaga agaaatgaaa gagctgagat tttattttgt gaatcggaag aggaatgtga 420 atcttttttt agttttatgg aaggttatta tagattgttg gttaatcaat atgacacaat 480 actttatgat actaacagca ttcttaaaga taacatggta catggcccta tcagtcaaaa 540 agttgctgaa aatatcataa cagcaaaggg gatagaagaa ggaaattatt tgtttcgtga 600 acactgtcaa aagtactttt tctttgtttt atcatatgtt tcttataatg aaacaataaa 660 acacattgaa attgaatatt gtcaagaaga ttctgaactt ggttactatc ttttgaatgg 720 aacaaagtac ccatcactca atgtactgca aaacagtttt aaagatgcaa aagtgaagga 780 tgaactaggt gttgtcctta aaaaaaatta cgtaccaaga caatctggaa acattccatt 840 ttacatggag ccaggacata ttcttcataa tattgcaaat aagcgtaaca tagcattatc 900 aaaattacct gctgcacctc ttttgcttgg ttcagatcag ttaagatgtg aacaatggat 960 aaaaaaggga gcttttggag atgttatgaa gtatgttaaa aaggatgaaa ataaatcatt 1020 ggttttgaaa agatttactg atattcctgc agaacttgtt aatctagttg ctcaatcttt 1080 aatgtgtcta cacgaccgta agcacattgt ccagtttgtt ggactttcct tattggataa 1140 tggtttaatg ttagcgtttg agtacttgga attaggaaat ttagaaaact atttaccaaa 1200 gtcaagagaa acgattacat atgggttgct gctcaagtat cttcgtcagt tgggtgatgc 1260 agtaaaattt cttcattcaa aggatttagt gcacggcgat ttgcaactaa aaaacatata 1320 cctttcagat ttacaaacta taaaactttc caattcttgt attatgtgga agttgtctga 1380 gcatattgaa aagaattatg ttaggaacaa ctgcactcat tatgctcctg agattttctt 1440 aaccgacaaa ccagtatata caaaagaaac tgatatatgg gcatttgctt taacaatttt 1500 acaattattt acattggaaa aaccattcaa aacaatgaca tattttgaga tgaagaattt 1560 ttttcatgag catttgaata aaagttcttc atatagttca cctttggcat atatgaattt 1620 agaatcgcca tgtcactgtc caacgacatt acgtaaactg tttaatgata tgctatactt 1680 caaaccaaac gaaagaaaga atattgtaga ggttcatatt aacatcatcg atcttttgaa 1740 agatgactgt gataaagcac aagaagtaaa tttttcaaaa gatttaataa gtacgaaagt 1800 taatcagaaa acaatagata ataatgatgt agatacctat cagcaatcat tctatagaac 1860 aagtttttta ccaaacaaaa gttgcagcat ccaagaccaa gttgaaaact taagttctga 1920 aaaattaagc tattttgata aaaataagaa taatcaaagt gaatctgata ttgcttctat 1980 tttacttgat ttgtctcaag caaacagttc ttctccttat gttgatataa aaggtaattc 2040 acatattcaa caaataaaac catatattca aacttttaac agtgctgtaa gcactcgcga 2100 ggattatgct ccaaagttag atagtttagc atctgttgca aaggctgatt ttgctaaaat 2160 atctcctcca ccaactccac cagattctcc agagcaagtt cgtcgtgata cttctgatca 2220 gccaaagcgc gagtggttcc gtaatccaaa aacacggcaa tatttacagg aacgcattaa 2280 ttttgagaga gaacgctttt atggaaaacg cagtatttcg tttcaggaag aatctaatga 2340 atataccgaa aatactaaaa aaattgctaa aatggcactt caaggtccaa atccatttaa 2400 tataggcata agtttacctc atcgattttt tgggaaaaaa gacgttcctc tgaatcctcc 2460 gccattctct tgtgaacttt ctagattttt taaaaatgag tttgaagaga attctgtcac 2520 acaggatgac aacgattttt caaatccata caggtcatta caagatttag aattgataag 2580 aaaaaatata tttaataatt caaacaatgc ttcaaataaa acgcaaagtg aaattcaatc 2640 tgttgttaaa gattttaaag atttgcaagc aagtagccct aatcctttta tatcgcattt 2700 aagtacctcg cacgcagctg tcatctctac tattaatgtt ttcacaaata atcctggtat 2760 agcttctgtt ggtactactt caacaattca gtctagtttt caaggtggta agccgattgc 2820 ttcaagttcg gctaaccgaa gttatggggg tcattctgag ggtaaaagac agtcaaagaa 2880 aaataaagat tatgcttcaa aaacagatat ttattctgtt gcagtttcgc atcaaagttc 2940 tgtaactaat agtttatctg aaaacggaca aacatttcca atgttgaaac aacaaaatat 3000 acagcctcgt ctgcttggtg taaatacaag attttccaca tcagcaaccc aatcttctct 3060 tcattgtaca atagcacctg cttctagaac tatatacatt tctcataatt ctactcaaga 3120 acctattcag ctttttccta gagctcactt taatggttct ccagtaacat ctattcagtc 3180 ttttcaagtt ctaaaccctc agtttattac tggatctcca attatttctc aagctacttt 3240 tcaaaaatct gacctagcaa tgcctctaaa accttctata gattcaacaa tgctctctag 3300 gtcttcatca ggtcaatttt cttctccttc agggatcgaa gagtctcaaa acaaaaattc 3360 aatgtttact ttttctgaag agcatttgcc atccatgttt cgatttattg agcgtaaaga 3420 tttaaaaatt gaggagagtt taggtcaggt tccagttgca attaaaaaat tactacaaaa 3480 agacacaacc tctaatattt ctaaagacat acaagatttt cgtagagaag caaaaacaat 3540 ggccgctctt tccaaaagag aaatttcaga ttcccaatac attgttaagt tgcatggagt 3600 gtgtgcagaa ggctcagaca ctgatttttc aattgtcact gagtatttat caatgggttc 3660 tttagaccag tttattccat taagaaagaa agagttaaat tcaggttatt tattggttta 3720 ttgtaagcaa attgcgcatg gtgttgacta cttacataca caaaatattc tccatcgtga 3780 tttagcctgt agaaatgttt tagttgctga taaagagctg gtaaaattat ctgattttgg 3840 tttgagtcgt tttttatttg ataaaggtta ttatgacaat gaaaagttcc aaaagttacc 3900 tgttcagtgg ttagcacttg aaagcatgga aaaatgctta tatactcaaa agactgatat 3960 ttggagttac ggagtggttt tatgggaaat gtttaccttg tttcaaacag aaatcaaaga 4020 cttaccagtt attccatata aagacatccc tttcattgat attgatttag attttttaga 4080 tagaccttac gttccggaaa aaatatataa aacatcacaa atttatattc ctcgagtggt 4140 agaaacaaga ccactcgaga gggcagatga aataatgtat aatggtgaac ttgattattt 4200 tgatgacatt tcagaaatag atgacgaaca agtacggata ttagacaata taatgatcag 4260 aatagaaaga gatttggcac gtcagatgtg a 4291 <210> 48 <211> 1494 <212> DNA <213> Hydra magnipapillata <400> 48 atggatgctt cttgcgatat taaatgtttt cctcaatccg aattacagct tgaattgagt 60 catgctagcg aaaatgaaac caagaacaag tctattgtgg tcgaactttc tgaaaatcat 120 tatggtgggt atgacgaaaa ttttgtaaat cctcttccta gtgatcttaa atgtcccgtt 180 tgtttcttgg cattacgtca accaattcaa accgaagagt gtggtcatcg tttttgtgaa 240 tcgtgtatca aaaatattaa aagaatcgaa ggacaactat tatgctctct tgacagacag 300 atgattgact taaatacgaa tttaagcttg agtatttctt tcctgggtcg tgcttttggt 360 agcggtctta caacctcagg ggaaaatttt gaaactgcag atggaattgc atctgaggtt 420 gaagttgata tactaatatc aatagctgca ttagattggg ttgctggacg atccgtaaca 480 aatgatggag caaaatctgc atctgaaaat atatccttat tatatggcaa tattcctgcc 540 actctaaaac cagaagatat attttgttgt gtatttctag ataaagcagc aaaacgagct 600 atactgtcgt tgcctatacg ctgtcgatat catgaacgca actgtgagtg gattggggat 660 ataagtacga ttgatgacca tcttaaagaa tgtggttttg ttgatgtttc gtgtcctaac 720 agtcaatgtc agatagcaag ttttactttt tatatacaga tgcctaaaca tgtcgaaaac 780 gaatgcccag ttaatgttca atattgtgaa tatgctaaaa tcaaatgcgt ttttaaggga 840 acccaaagtc aaagagatgc tcatgttatt tcttcagcaa accttcatct tacattgtca 900 attgaaaaaa ttacaaaact tgaaaacaaa tttagttacc aggtagaaca agcgacaaac 960 ctggccataa aatatgatgc attaaaaaaa aaagaggatt tactggaggc tttaattcga 1020 actgttgaga gacaaattag tagtttagaa aacaaacttg ttaacattga cacaaaaagt 1080 tgtttgttcg aatcccgttt accaaaaaac gagtatttat ggtgtttgga cagtatagat 1140 tttctgataa ataaagagaa aaaaccaatg aaagatctaa acatttgcag caccccgttc 1200 tattcagctc cgtttggata taaactttct atgcaaatct atttgaacgg attaggcaaa 1260 tacagaggaa caaatatttt attgtttctt aatattataa aaggtgagta cgatagttta 1320 ttggaatggc catttacaaa aatggtcaaa ctttctctac ttgatcaaag cgaagcaaga 1380 gaccataagg tatttctaga taaagcagca aaacgagcta tactgtcgtt gcctatacgc 1440 tgtcgatatc atgaacgcaa ctgtgagtgg attggggata taaaatacat gtga 1494 <210> 49 <211> 1725 <212> DNA <213> Hydra magnipapillata <400> 49 atgaccgata tcaaaatatc ttcaaatatt tcagggagaa atttacttcg ctttcatccc 60 gacaaacaaa gagaaaaaat aagtgatgcg tcctttaatg gtataatatt tgtaaaacaa 120 gataatagtg tcaatcttag caataacgaa tcacctcaaa tgtctccgtt taaacaaaat 180 gaggagaatg catttaacaa ttttgctact aacaaagttc tagaaactga tattgttgaa 240 tttttagata tttgtgaaac tgtaatatgt ggcaaagaag atgaaaactg caataatata 300 gttaaagaag atcatgttat agttaatcaa aaaaagaaat ctgcagtgca ttcctttaaa 360 aactttgttc gaggcacaat cattccaatg ccatctgaaa ctaaagaatg gctgggcaaa 420 cagcctccag actgtgcaat cgaaatggag aattgtgtat taaacaaaga acaaagtttc 480 tcaattaaaa ccgaagatgt tccaaggagg aaaaagcttt caagtgttat taatcgcact 540 aaaaaaactc gatcaagaat acataacgaa ggtgcttctt tatggacacc aatggaaaat 600 agtcgttgga agtctggaga aaaagttgtt gaactaaaag atatttcttt gcaccaatta 660 actgaatcag agcgtgaaaa attgaaacag tacggattag aaagattacg agaaaacaat 720 tttgattgtc atttaataat ccccaaagaa ttattgtttg catcaggttg cagtttgcac 780 catgacagta aagaaaaagg tgctaaagct gtcgtacaaa tatcagaata catgagcgca 840 tctcctaggc cttataaccc tgtgttttcg gtggatctaa accaagttat tgagaatgat 900 aaacttattg aaaaagcgca aaaagaggca atgcaaaaac ttgataaaaa tgaagagctt 960 ggggaaaact ttgaaacgaa tgaaaaagat caatcaaaca aaaacagtat aactagaact 1020 aaggtctgtg aagaggcgtt tacaaccgag gatcatgaag attacaactt gaattatttt 1080 aggacaatat gtacgctgca agaagaagac gaggatgaat ggagcaccaa taaaagaaaa 1140 tcaaggttaa ttgaagctct tgcactttct tcaacatctg cttcttcttt gtgcatactt 1200 gataaagaaa tagagtttaa acaacttcct cttcaaattc caaaagtcat tttgcaaaca 1260 acagagttca taaccaagtt tgccttgaat acagttggaa tatttcgaac aggaggttca 1320 aaaaaacgag tgactcaaat gaaaagtgat tatgacagag gattttttgg tgtcattaat 1380 gaagaaagta atccaaacga tgttgcagca cttttaaaag agtttttaag atgtttacct 1440 gatccattgt tgactagaga actttaccaa gtttttttat ctttagcaaa aaaagaaaca 1500 caaacaaaag aagaaaaagt tgaaattatt cagcagctta tctggttgct acctgtggct 1560 aatcgagata ctttagaatg tctcctaaat tgcttgcgaa tggtagctga ccattcccaa 1620 aattcaaaag atgaatttaa taatgagata tgtggaaaca aaatggactc tctaaaccta 1680 gccactttaa tggctccaaa tattctacac agatgcaaag tatga 1725 <210> 50 <211> 2091 <212> DNA <213> Hydra magnipapillata <400> 50 atgataaagt caaataccaa atcagaaaca aatgaaaaaa acagtaaagc atctacgaat 60 ATTGACAAA ATACTCAAGT atctacatta tatggtacag agttaaatga ctcaaatata 120 aattcaacag aaagtgacga agttaaattg attagagaaa tatttaggaa gagaggttat 180 aataaagaag tcagacctgt tgtaaaaaaa gaagatgttg tacaagttgt gtttgattta 240 gcctatacac agttacttga tctggatgaa aaaaaccaag tgcttgttag taatgtttgg 300 gttcggatga gatggtacaa cgagctatta aagtggaata aggacgattt tggtggtttg 360 gagtcaataa atattgattc atcaaaaata tggataccag atattgttct tcaaaacaac 420 gcagaagaag ctcttggtgc tggtcaactt gatttgttta atacaaaagt aattttaaac 480 tacaatggag aaaaccagtt ttatgctccg gctatacttc ggagtcgttg cgaaattaac 540 gtggaatact ttccttttga cgatcaaaaa tgtcagctct catttttaag tttaagctat 600 gacaaaatga ggcttaacat aaaaaacaaa accagtcagg ctgatagaat attttaccta 660 gaaagtgcag agtttgaatt gctaagcaca aaagctgaaa acaaagaata ctatcacgga 720 gactattgtg tgccatatat gaacgttgta tatacaattc atattcgacg aaaaacctta 780 ttttatttca acaacttaat tggtccgtgt tttctcatta ctatgttgtg tttatttaca 840 tttcttttgc ctcctgaaac tggtgaacgc attactttag ttataactac tctacttgct 900 atgacggttt ttatgctgac cattgcagaa aagacaccaa tagcatcaaa agcaactcct 960 ttagttacaa aattcttttt ttcgtcaatg gtaattattg gtctatctct catagcaacc 1020 tgtatagtat taaatttgta tgaaaataga aattcacgga gcactgcccc tcgaatattg 1080 agagtaattt tatttgaata cgtagctccc tttttatatg tgaattttaa taaagattta 1140 ttacgatcca ataacatttt gtatcaggta aagcaaggta atatgaaaac tcaaaacgga 1200 cacttaagta aaccattttt atcaaataga gcacagacat ggtgtacaat tccgcaaagt 1260 caaaatcaca ttttgagtgt gccaaaatgc ataataaatg gaataaaagt acttgctgat 1320 cgagctaaaa aacgcaacga agaagagttt aaaagtgagg aatgggttac aattgcaaag 1380 atttttgatc gattgttttt tatagttttt cttttcacta ttgtattatc aggagtcggt 1440 ttaatttgta atgtattttt cagtggttgt tgtgcgcctt atatgaacgt tgtatatact 1500 attcatatta gaagaaaaac gttattttat ttcaacaatt taattggtcc atgcttcctt 1560 ataacaatgt tgtgtttgtt tacgttcctt ctaccgccaa aaactgggga acgcgttact 1620 ttagttataa ctacgctatt ggcaatgacg gtttttatgc ttacaattgc ggagaagaca 1680 ccaatgacgt caaacactac tcctctcata ggacaatttt ttgtttcttc catggtaact 1740 ataggactta ctctaatagc aacgtgtgta gtacttaacc tgtacgaaaa taatcattct 1800 cccagtactt tgcctcgatc agtaagagta attttactgg aatacttggc tcctttattg 1860 cgtataaagt ttaacaaaga ttttctccct tcaaaaaaca tcttatgtgt accaaaagga 1920 ataatggacg ggataggaat acttgcggac cgagctatac agcgtaatga agaagaacgc 1980 ataagccagg aatgggcaac aattgctagg atttttgacc gattattttt tattgtcttt 2040 ttaatcacta ttgcattgtc agccgctttt atttattcta atcaaacata g 2091 <210> 51 <211> 574 <212> DNA <213> Hydra magnipapillata <400> 51 ctatatagct atatacttag aacaatttta tttaacgata agaatttcgc aatcatgttt 60 atgaaaatta ttttaatttt ctccgttatt cttgcagcta ctgctgatga gtttttagag 120 tttgaagatc aatacgaatc tagcgacacc attccatcta attttgacaa cgaaaatcaa 180 ttcgagacta agattgatta ctttcctaaa agtgaataca aaccaaaaaa gattttttgc 240 ttaggtgtcg atgtgactaa aaaatgttta gaacgcgctg atggcaatta cgcaattgat 300 agcaaaacaa aacctggtta tgtagcttgt gtagacatga atccaatatg tatgccatgc 360 ccatatggaa tggtattttg tgataagact ggcaacaaag gtcttgggca atgccttcga 420 caatgcccac ctcgctcgta ctgatcatgt ttctatgaag ctaaatttga atagtttgta 480 aaacatatga attacaattg ttaattaatt tttacatata atatttgtaa tgaactacac 540 taaaagtttt aaataaatac atgtgctatc aata 574 <210> 52 <211> 9024 <212> DNA <213> Hydra magnipapillata <400> 52 atgggcaaag ctatgtatct gatcaagtta tatgggcaaa gctatgtatc tgatcaagtt 60 atatgggcaa agcttgataa aatactgaaa tgttatgatg aatgtgtcaa gcggggaagc 120 tacaaacccc ttgatgaaat ttttgatatt actaaagtgg atggaacatg gttatcttca 180 gaggacaagg gattgtacca tctccaagtg gaaagcaaag aaaaagtgaa atactctata 240 ggccaagcgg ccaattcatc aattaatatt tgggtatttc tatatattct tgaaattctt 300 cttggcaagt tgggatgttc tttttggttg ctatccggaa tcaatggcaa cacacctatc 360 cagctatata atgctataat aaatcataaa gactttttaa tattgcacaa gaagtatcaa 420 gatacagcat atgttgagca agagttaaca tattcatcct attctcatat ggtatttagt 480 catactaaaa atgtaaatga aaaagatagt tgcaattgtg ttttgcaatt attctcgtgg 540 acattgcctt ttcttaaaag tgtaatgaat attggaatcg aagcacagac tttatttggc 600 tatattttat tttaccatac tgatttttta ggatttaatg ttcaggattg tactcctatt 660 cttcagttaa ttacagggtt taatgttcag gattgtactc ctattcttca gttaattaca 720 ggatttaatg ttcaggattg tactcctatt cttcagttaa ttacgggatt taatgttcag 780 gattgtactc ctattcttca gttaattaca ggatttaatg ttcaggattg tactcctatt 840 cttcagttaa ttacaggatt taatgttcag gattgtactc ctattcttca gttaattaca 900 ggatttaatg ttcaggattg tactcctatt cttcagttaa ttacaggatt taatgttcag 960 gattgtactc ctattcttca gttaattaca ggatttaatg ttcaggattg tactcctatt 1020 cttcagttaa ttacaggatt taatgttcag gattgtactc ctattctcca gttaattaca 1080 ggattaaatg ttcaggataa atgcaaagtg tctgacttgc tgtatcatct tgcttataaa 1140 aatattgttg aaattttcag tttcacttta aacatgaatt ttttttggat acttctgcca 1200 tttattgatg ggctaccaga tttgctatta aaacttacta ttaatgattt tactggtgtc 1260 atcagtttta ctaaacagat agcttcattg gttaatatgt tagttgcaaa ggctcttggt 1320 aaaaagtata tttctaagga attgagttta tcatttcaat caaattttaa aaaaatgcta 1380 atgcaccaat cttatgatct aaatgtttta aaaaatatac ttcttattat acttcaaaat 1440 tctaccaaaa ttaaaccaga gccactaaca gaaaataatg atttagattt ttctgtttct 1500 gaagaacttg atacagaatg cctctcaata acaaagtctt ctccgataga tatgaggaga 1560 cttggttcac acagtcctat ccttattagt gatgaagaag aagttgacct tgatactcat 1620 gctattaaca actttaattc tcttgcctca gttcctattg tttatagcta taacaccatg 1680 gcttccggga ttaagtttga agatttaaaa aataaaacta taaaacctga cgaatctttt 1740 ttatcgataa aatataaaga ggtaacacct tctgtacctc aaagtaatgt tcctaaaatc 1800 tttgaagcat caaaattttc tctctcagaa tcaaatcaaa gaatttctaa cataggttta 1860 gtacctaaat ctaacgattc aactttatta aatataattg atttagaaga ctctgagtct 1920 ttgaaaatta atcaaagtat aaaacataaa aacagtaatt ttaaagctaa tgagaattta 1980 gataaagact tgaataaaaa taaaatttta gtcagccaaa cagaaaacaa tgttttggca 2040 aatttcgaaa ctgagaaaga tatgagtaat tttgttgata aatgttttga tatttcttct 2100 aattttaatg aacctctgct ggttaaaaag atagctccaa aagcatctgg ttcaacttta 2160 atgcaaagat cgaatacatt gcgaagagaa aaaattgagc aagaattaac taccaaaagt 2220 aaacctctaa agttaccaaa tctcaacagc aaagtacttt caacttctag ctttcataaa 2280 ttaatattta aatggaaagt agaatggttt gaaaaccaaa aagaacctgt tgaaacaact 2340 aaatgtttaa agaatgttgg tattaatttt gatacttatg atgaatatta caatacattt 2400 tgcaacttaa tactttgtga agcgcagagc cttgttagta aagaatggaa agaaaaatta 2460 tattcctcta aaatttataa aattactgaa attaatgaac ataaaacaat agatacaagt 2520 tatcacatta aatgtgagta tgaatgtaaa gagaattatc tagactgtcc tagtgaagat 2580 gatttagtat tggttcattt tattaatgag aatcaatcag tgtttggtat agttgaaaaa 2640 gctgttataa ataaaagata caaaaatagt tttgtgccaa cttatgtttt aaatataaag 2700 ttagcagaaa tgtctaatag taaaacatac aaaattgtta gcattaagaa aattgcttcg 2760 ctaagaactg ttataagaca atttaatgct ttaactgtgt ttaaaaactc tttgcttgca 2820 gcagatatat taaaaccatt tagaatgaaa acatataact gcaatgaaga acttcttcaa 2880 aaggataaat ttaaaagact ttctaaattg aatctaatgc aactccaggc actttctact 2940 gtttcgcgtg ctgtttgtca agtagatttt gttattccta gaattgtact attacagggt 3000 ccaccaggaa ctgggaagtc atatactata aagacaatta taactcactt aatgcaggag 3060 ttttataagt caagagcatc atcaaatcaa aagtctcaac gtattttgtt ctgtgctcca 3120 tcgaatgctg ctgtggatga aatagttagg cgcttagtac agagtccacc gtatagagat 3180 gataatgata gtcatgctat aaaacatgga aattgtggtg attttaatat agttcgagtt 3240 ggtcagaaga cacaggtttc atctgacttg gtgcagtact ctttagaata tttattagaa 3300 cgtgaactta agaactataa gtcatcacat aataaatcaa aaattataga gcaaataaaa 3360 ttgttgaaat ccaggctaca agttatggac attgaatgtc agaaactcag gatgtcaaat 3420 aatcaaaatg aaagtgaaca gtacatggtc aaaactgaag agcgctctcg aatgcaaaaa 3480 tcattagatg agcttaatta taagcgtaac agtttatcag aaaaaggaga tgatcaaggt 3540 ctttatagtg aagaagaaca taatttgcag cgcagtttgt tattgcgagc agatataata 3600 tgttcaacat taagtggagc tggatccaag cctatggttg atgcttttag aagaagccgg 3660 gaaattccct ttcgatgtgt tattattgat gaagctggac aatgtacaga accagatgca 3720 cttataccac ttcaatatgg ttcttccaaa cttgttcttg ttggtgatcc tgcacagtta 3780 ccagcaactg ttatttcaca aagagctggt agatttaatt tgggtcaatc attgtttgaa 3840 cgattgtata aaggcattat tattaacagt gaagcaggag tgcgtccagc gattttactt 3900 aactatcaat atcgcatggc acctgaaatt tgttggttcc caaacaaaag gttttataac 3960 aatgaactaa agtctaatga agcgctaata aagaaaaagt ctgatttaaa accctatgtg 4020 tttcttaact tagatgaaag tagagaagat aaaactcgaa tgggaggaat acataatcct 4080 gtagaacgtg aacacatcat tgcaatatgt gaaaaaattg ttacagaaaa aaatgctaat 4140 gttaatgaaa tagccgtgat tactccatat agatatcaag caagtttaat aaaacaggag 4200 ttaaacaaaa agctagcaca acttgaaggt atagaagtag atactattga cggatttcag 4260 ggtagagaaa aacgaattgt gatattttct tgtgtgagag cttcaaatca ccaagaaagt 4320 attggttttt tgagtaatcc acaaagaatg aatgtagctt taacaagagc aaaagatgtg 4380 ttgatcatat tagcaaactg taattcaatt gagattgatg aagactggaa agctcttgtt 4440 gatgatgcta aaagcagagg ccttctattt actgttcaaa attgcaatga caccctccaa 4500 tgcatattta atacctcaga tgtagcattt aaacaaactg atcacttaga agagaataaa 4560 accaagaatg taacccataa tcaaagtata gcaaagaact ttattgatac ttctattata 4620 agttcagata atccttgttt tcctaattac ttagacaatt tacaagttta caatattcct 4680 gatatttcag tcaaacctca gtctgattta gagaaaactg tgatggtaaa agaatcttta 4740 gaaaaaaata gaaaaactca atgtaatttt aattctgaac tatgtacctc taatgtttta 4800 aatacacttg tgatggataa atctgcaata aatgaacctg cagttagtat gtcattaata 4860 tgtaattctg tattgaatga acctgttata aatgaaaata ttaagaaaag tcaaagttgc 4920 tataatgctg aacgacatat tttaaatttt ttgcatacac ctttaccaga taaaattaca 4980 ataaatgaac ctgctgttaa tgtaccttta ctaaatgagc ttgcacctaa gatgtctctt 5040 atggataggc ctgaaaataa tgaaacttat gcagttaaca tgtcagcaat atgtaaatct 5100 gtaattgatg aacctgcaat aaatgattgt tctaagaaaa gtcagtgttg ttataatgcc 5160 gaactacata cattaaatag tttaagtaca catgtattag ataaatttgc aataagtgaa 5220 cctttagtta atttatcttt aaataagaag cctgcaataa ataaacttga agataatgtg 5280 tctgttatgg atgagcctgc aaataatgaa aattcattaa atgaggttgc atttaacata 5340 tccacaatgc atcttaaggt gaatgaacct gagatgggca aacctttgaa taatgaatct 5400 actctaaaca aacttgcatt taaagaacct aatcaaaatg gagtttttat atttgaaaaa 5460 gattttaaaa tggatttgct tgaggggaac tcagatgcag aattgaataa attagctgct 5520 aaaatctctc ataataataa taacatttca gaacatatat ctacaaatgt gcaattgcgt 5580 aaaacacaaa gtactacagg caattcaaaa aatgacaaaa aacagcagtt agacaaaaat 5640 actattttgg tttataaaaa tgaacttatt agctctgagt taaaaagaaa gtttgaaagt 5700 attaaactta aaaagaatca tcaaaaacat atttcttctt taaacgctca ggtagaaaat 5760 gtaaaaaata gttatgataa cattgataat caagaacctt acgtaagaaa agtaacagag 5820 gatgttccca taattgaatc tgataaacct aatctaataa aaaaacaaca ggaaaaaaaa 5880 aagaaatata agaacgctaa tacaaaaaac caagaaaaca ttgataatgc tgacattcaa 5940 gaaccttaca taagaaaagt tacagatgat gttccaataa tcgaatctaa taaatcaaag 6000 aaaaaacaaa aaaagagaaa gaaatataag aactctgata caggaatcca ggaaagtatt 6060 aaaaaatcaa ctgaatatag tcccgatatt ataattaaaa aaatactgaa ggaacaatta 6120 aaagaagatt ttgcaataca agagaagata attggagctg taattgaaac acttggtgat 6180 aagcttgtta ataaaatagt atctaaaagt aaaagtgtta ttgatataga atctccaaca 6240 tattcaaatg agtctaacag tcctttagaa acagattcta atgttaatat tacagaaaaa 6300 tcaaaacaat gtgctttgca actacgcata aatagtagta acagcaaaga aagtgatgtt 6360 caacagaatg tagttgatac atctgatgga gcgcaaagtt tccgcagaaa taaaccttct 6420 gaatcttatt ataacttgcc tcaccaccat tctgacggag cttattgcat agacgtatgt 6480 aaacgtgttt tagagcgaga gaaaaaaaga cgtttttttg ctgaagatga tttgttaaga 6540 aaaaacagtt ctttacctaa attaattgaa tggaagttaa ttcctctaga gatagaaact 6600 cccgttgtgt attctaaacc tgagcttagc agttcatata acttggtatc tgaccctcgt 6660 ttaagatcaa ataattcttc agttgttttg ggtcatctaa atcttacaaa aaatgacaac 6720 actcaaaatg accttggaac tttaagaaaa gatacaggtt tttcaattct aagtcaaaac 6780 tcagaacatt ttcaacaatt agaatgtgca gttgaactgc ctaaacttcc agttttatca 6840 gaagctgact tttttccaca tgatgcaatt gatgaccatc actctactcc gatggacata 6900 tgtgaagata tcaaaagtcc aattctcatt aatgaaccaa tcagtaaata tccattaata 6960 ccaaatcgtg ggttaactca aagtgaaggt tttttttcat ctagtagcaa atttttggga 7020 tttaatgaca attttttgtg tcaaccaaga ttagattttc aaaatcaaga gttcttgtgt 7080 gatctaacca aagaaccaac cgggtctaaa ccatttcctt caaaacctcc attactgttc 7140 aaaggtagct tcgataatta tacgtctatt accaaatttc aagatggaat tcaaagtaaa 7200 tgctttgatg atccttgctt attaagctgg agagctgttg aaaaaattaa agagaacaag 7260 tactttaatg atattcatga aactcagcag gtgttgctaa cgacatcaaa aagaaaagta 7320 gcttatatat ccccacaacc tattgaaaac catcaaaggt atatatcgcc acaatctatt 7380 gaaaaccatc aaagcaaaag aactaatatt caaagtgata ttggaatcca aaattatgaa 7440 aagcaagata ttgactcagt tataaacatt aatgaaatta tgaatgctac tcttcaagac 7500 ttagccgtga agaacgtgtt tgaagattgt gttcgacaaa taagtttgtc taataattac 7560 gaaaacaaag aatatatggt gaactatgtt aaaggttttt taattcgtac atttagcaat 7620 agtataaaaa gtgattcagc ctcagaaagc acaaatgttc aatctcccat acaggattta 7680 tcatctgaac ctgtaaaaat gtacaatttt aatgagttac atgagtttcc agaagaaaat 7740 aagttgtatt ctaataccaa ctgcaaatat gaacaaccaa aagataaaaa atttaagatt 7800 actccatccg acttgaactt atcggaaaat attatcccta gttttgttca taatcaaaaa 7860 gtactaattc aatctccgac ctttaacgaa cccattcctt taatttgcga tgaaagtaaa 7920 gtttggcatc aagcacaaag taaattaccc aatagtatta ctattcaaat taacaatgag 7980 gcaattcaaa ctaaaaatga cacaattcaa agtaatgatg acgcaattca aagaagaatt 8040 cagcggtttg gtgttgtgca gaaagaagaa aactgtgtta ccagtccttc aaaaaaacgt 8100 tttaaatgtt ctttgatttc agagcatgaa aaaacttcaa tcaacattac cgataacctt 8160 tctaagacaa aatttagtaa ttcaacattt gaatgtcgag tcttaattga agatataaaa 8220 gataatgata tagattttac atctaaaaaa gaatctttat caaataatga caagcaatta 8280 agctacgcaa gaaaagtcaa acgtgcaaaa atgccaagat cgagtaatcc aaaagctgta 8340 atcaaaatta attcaaggca attaaaatct tcaaacaaga attatttaga aaatttatcg 8400 cccaaaaaaa tcaaaatgtc ggttaaaagt gatgatccaa aatcggtgga tacgaagaaa 8460 aattgtattc agaactataa tgataataat gacgtaagta ttgttcgaag cgaaaattta 8520 tttagtttat ctgcgtcttc gaatgataaa aagacagatg ttttttcccg acttggttca 8580 cgcttatcta cagataatct ttctgaaatt tcagctacaa acaagccggt ttataaacca 8640 aatgaagtaa gaagttttcc tttaaattat cgacaagact caacacattt tgacaacacg 8700 tgtaatagaa aacagttttt aatagaccat aggagtttag gcgttgtcaa accctataga 8760 tatgaatcac cgggctctga aacatttcaa aaaggttaca gcgaaaagaa atcgtttatt 8820 aaaaaaaact ttaatgaaaa gacactcccc cacttatcgc cggataaacg gaaatataga 8880 ctaaatgaga gttcttactt tgaaggcact tcttacgatg agagaccata tagtaatcat 8940 gcgacagatt actttccgaa tatggaagta agaaggcatt ataaagttac acaaaataag 9000 aaaacttacc gtttgcgccc ttag 9024 <210> 53 <211> 3714 <212> DNA <213> Hydra magnipapillata <400> 53 atgaaaactt caaagaaatc ttgtgctgaa attcaaaaag tgtttcggga cagacaatta 60 gaaaaaaata cagacactgt ttgtgaaaaa gaatgcaaac attggcatat gcggcgtgag 120 caaaagaagt cactggtacg cgaattgagt aacaataaca aatgcaaaca aagagctaga 180 gcaaagattg gttaccgaaa aacaaaggct tatagacaaa tacaagcatc gacggaggag 240 ttggaaacag ttaattgttc agccgaaaaa tacaaaaaac attggttaag gcttaaatgt 300 tccctgcctg gttcacaagc taattcacca acaaccactg aatcacctaa aacccctaac 360 tcttctcttt catcaaaaaa tataactcca aatagcgagg accatcgcgg taatgcatcg 420 cttgatgttg aaacacagga tttaatcaga gagtttctta catgtgatga caattctaga 480 ataacaattg gaaagaaaaa gactgaaaaa aagattgtaa caaagtcttt ggatggtgtt 540 attatgaaaa taagtcctaa tggtgagaaa gaaagcatca atagccgttg cactgatcta 600 tcaaaggaaa ttgtatctcg acttggtgta cctatgtctg tacttgaaaa tgttattttc 660 tgccatcagg aagatgctaa ttggccacta agtgaaggga aaattttaaa acaaaagttt 720 gattcaatat ttgctgcaac aaagtatata caagctttgg agtgtatacg aaatctccgt 780 aaggaccagg ttcagttagt taaagactat aatcgagacc ttgtacatct tacacaaaag 840 aaagacaaag cgcaagagat tcagtcagat ctttgtagag cacaaggtca attacaaaca 900 actaaagacc aacttaaaaa aattgtttct gcaatgcaac ctattgaatg tcaactggaa 960 caacttaact ctgtatcaga ggaggttttt aagcgtgaga aagaattagg gactttagaa 1020 gcaactaaag aacatttgaa aaaaaatgct ttacaactga gaaataatat ttcaaacttg 1080 tttgaaggtg aactaagtga gttggagaaa aagcgtgctg aatttagtgg aacattacaa 1140 ctgcaaacta tgataagcgg atttgaaact gatataaaga aactaaatga aacacgagct 1200 aaccttgata aagaacatgg aaagcttcag caagaagctc agacacatag tgttttggtt 1260 gcagagagag acaacttcat taaaaaaata tcttcaaaac ttgatctatc aggttttaat 1320 gaggatactt ttactcccga atgtgccgat cagtttttac aaaaattaaa agatttattt 1380 caatcaattg tttcagaagg aaaaaaatct aggagcaatt atgaaagcca gttgaataca 1440 ttggaagagg aaataaaaaa ttgtgaaaag gaaattaatg gtattgaggg ggcagtaaat 1500 gaaaagcaaa gtataattaa caataactta gtgaaaataa gaagaatcga aaatgatctt 1560 ggttcactat cttctactgc aactcgactt caatctcttg aatctcagtc taaaactgta 1620 aaggaagaat tagatgagta taaacagagt gttgattcaa acactataaa taaagacata 1680 gagaggctta aaattaaaaa aaaggaggca gatgctaaac ttgccaagtt aaagcaagag 1740 caatcaatga tctaccaaca atcaaaagca cagaataatg ttgagtcttt tctgaaagat 1800 caagtatcaa agcgtgataa cattgaagca ttgttaaaca ctcatcttcc taaattagaa 1860 tcattatttg gattcaaaaa agttcctcct aaagaagatc tgaagaaaga gttatacact 1920 tacattaaaa aaaaagaatc agaagtgaaa aatgaacgac agaaacattt tgaagttgct 1980 caaagacttg cagggttcga aagtaatcaa aagacaatta taaatcaatt aaaagaaaaa 2040 gagctagagt ttcaaaaaaa agaaaaggaa gtgtataatg tttgtaaaga taataactat 2100 tcaacctaca aagattccat tcagcaaaaa atagaaaaga tttccaactc acttgttgaa 2160 atgggagctt ttgagaagat atatcaaaaa tatatttcaa aacttcaaca agaaaatagt 2220 caagaaaagg gttgccctct ctgtcacaga gagttcaaga atacaaaaga agttaaaaat 2280 cttatcgaag agctttcatg taaactggaa tctgttccca aaaaacgatt gcaaaatgaa 2340 aaacttatag aggaagaaaa aaatcttcat aagaagttag aaagtcttat ccctataaaa 2400 gcttctctcg atgaaaaatt taaaatagcc gagggtgatg aaaagaaggc acgttcaatg 2460 gaaattgata taagcaaaat aaatgactat atatcagatt taaaagagtt ggatactaag 2520 attgaacgag aacgaatcaa tcttgttggt ataatgcccg gccgtacttt acagtcaatt 2580 gcagatgctg ttaatgatgc tgagagtgaa gttggtaatt tttcaaagaa cataaatgtt 2640 caagaacaac tttttttaca aagtaaactt caagaacggt taactcttga aagtcaatta 2700 aaagagctta agtcaacaaa tgaaacatat aaccatgaaa taaaagattc ttctatcaag 2760 ctccaacctt tgcgttctaa actatcagta aaatctgctt ctaaatctaa agtaacaaaa 2820 gaaaaagaag aaaatgaaga aacaatcaga ataaaacttc ttgatatcaa agataatggt 2880 aataagataa aagaatggac agagaaaatt aacagttaca ttaaagaagg caaagaaaat 2940 gcagttatgg taaatcaaac gcgatccaaa agtgttgagt tggagttaaa aagaactgaa 3000 gaaaatcgta agaaaaggtc agaagagatt gcaaaaatta ctgatgagat ttctaagcaa 3060 gagattagag caagagaaat tgaggataat atcaaaatta cttctctgac attacagata 3120 aggttggcca gtgctatgta tgcaaatgca gaaccgaaat attgtgaaaa agttattgaa 3180 ataaagacaa ctgagtttgc tagttctgat cttgcaaaat attacaaagc attagatagt 3240 gctataatgc gttaccacag tttaaagttg gatgaaataa ataaaatagt taaagagtac 3300 tgggtaaaaa cttacaaagg aaatgatatc gatactatag aaatcatagc agaagatgaa 3360 gaaggaaatg gagcatcaaa agcaagacga tcatataatt atagggtagt catgctaaaa 3420 ggtggagttt atcttgacat gaggagcaga tgcagtgctg gacaaaaagt tttagcatca 3480 ctcattatac ggttagcatt ggcagagact ttttgcttga attgtggtat tctggcatta 3540 gatgaaccca caacaaactt agatgaagct aatattgaaa gtcttgctaa ctcactaaaa 3600 gatattatag aattgcgaca gtcacaaaaa aattttcaac tgctagtaat aactcatgat 3660 gagaggtttg tccaagagct tggaagatca gattatgttg atcccttatt ttag 3714 <210> 54 <211> 1095 <212> DNA <213> Hydra magnipapillata <400> 54 atgtacgtaa cgcttaattc gagaagttat cctgaaattg ttaattataa tctatcattc 60 ccagatcagc aattttcaag agtttataga gatgcatctc tatttactga aaataaacaa 120 tcagcaatat taaagccatc aatagtagat gttcaagtta aagcaacatt taataaactt 180 gttccagaaa atactatgga atataagata actggtggaa aaggtaacgc tgtccaaaga 240 tgtagttcct gcaatggaaa tggtatcaaa gtaagttttc aacagttagc tccaggaatg 300 gttcagcaaa ttcaaaaagt ttgctcagac tgcaatggcg aaggagaagt tattgatgct 360 aaaaacagat gtaaaaagtg tctcggtaaa aaagtatgtg atgaaacaaa aattttagag 420 gtacaagttg acaaaggtat gaaagattct cagaagatta cattccacaa tgaaggagac 480 caacagcctg gtattgaaag tggagatgtc ataattgttc taaagcaagt tgaacatccg 540 cgctttaaga gaaaaggcaa tgacttatac ttaaaattaa caattggttt aactgaagca 600 ctatgtgggt ttgttatacc aattcaacat ttagacaatc gtgaattact tgttacaagc 660 agtgctggaa aggttattga accagattca actcgtgtat tgctcaatga aggcatgcct 720 cattatcgaa gcccctttga taaaggtcac atgtttgtat cgtttgacat aatatttcca 780 caaaataatt acctagaatt ggaaaagtta aaacttctcg agagtattct tccgccacga 840 gctaaagtga ctgacattga tatgacacgt gaagacgttc aagaggttat gctcagtagt 900 tatgatgcgt caatggataa tggttcttcc aaccaacgtg aggcttatca tcaagatgat 960 gatggtgacg atgatggaca tggtccccat ggtcaaggga tgtcttgtgc tcaacaataa 1020 tatcggctaa cagtttgtaa atttcgaaaa tgttaaaaaa tttgtcaaat atattttgga 1080 cttgtcatgt tttaa 1095 <210> 55 <211> 3528 <212> DNA <213> Hydra magnipapillata <400> 55 tgctaaagtt attttctgtt ttttatgtaa tcttcaacaa tgtctggtaa tgaaaataga 60 gtaaatgttg tacaatgtca acttatgtac aaaagtgtaa aggatttgaa taaagtcgct 120 gaaaaaatag aagttgttat caaaactaat acacaatgtg cacaatattg caagtctgtt 180 aatgaaatct tcaaaaaagc tgaaacttat tttaaaaatg gagacgatga gctagcttat 240 atattataca tgcgttgcta tattatctat aaaaaaattc gagaatctta tgtgtttaat 300 aaagaaaaaa atacattttt atcactactt aaaaactgca atataaaagt tgctatagaa 360 aaagcagaag agctaagtga tcaattacaa aagagatata cagagcttgc tgaatgtcaa 420 gataaaagtc agaaaaaaaa tcagaatggt cataactcaa atgaggatca atttgacctg 480 attttacctg atgttccgac cgatttcaaa attagatctc ctaaaaatgc gcatataacc 540 aaagaaacag acaaaacttc taccaataat cacaatgaaa ttaataattt atcatctagt 600 ataaatcttg tcaagaaaac ttgtacattt atttctgtta ataaacttta tgaaattcta 660 aaggattgca aaagttaccc acagtctact ttattaattg atgtaagacc agttgaggaa 720 tacaatgaag caagtatttg ccatcaatgc atgattaatg tgcctgagaa tattttaaaa 780 cccggtgcaa atgttaatac aattgaaatg aagctaaatt tgtttacacc aacatgtcct 840 gttaaaattc tttcagatgt tttgctcaag tttgagggtg gttttcaatt gtttgctgta 900 ttaggtggtt ttaaagaatg gcatttatct tatccagcac ttacaacaaa tcctatatac 960 tgcaaacttc ctcaaaatat gacctttaat caagattttc tcagccttgt aaaagatcta 1020 tcatatccag agcttatttt tgacaaaaaa aaagatgaaa caaaaatagt tgacacatct 1080 tctgtggcta attttatgag acctgatcag atgcatgaca aaaataatac tgaaaaggaa 1140 aattacattg aagtacctgt tgaaaatcaa acaagaagta aaaatgtaac taataatagt 1200 aattcacaga tatctttata tgaaatagtt ccaaatagcc aaaaatctga agagtctaaa 1260 actcattttg acaaaaaaca ctcctcactt gaatcccctt cattaaattt agtatctgat 1320 aaaaatctaa ttaataatag agatataaag gagaatgtag gtaaaactat tgtagatatt 1380 aaagagaata taaataaaac tattgtagat atcaaggaaa atgaaaacaa tattattaga 1440 aataataata tgaatggaga taataccatt agaaatgata atgtgaacaa agataatacc 1500 atcagagata attcttcagt tgaaagaatt tcagataatg actatataaa tagttatatt 1560 aataaaaaag ataattttgt tcccagtata ccaactaact tagataagtt agtattgaca 1620 caaccagttg ttgggttaac cacatcagat aaactccctg ttaatgtcat atcccatata 1680 tctcctccaa tattgccagt atcaacaaac acttcaaccc aacacctaac taacatccaa 1740 aatttaacat taccagataa acctaaagtt ttcttacagc caaagtttaa agccaattct 1800 gaaaatcttg ctgaacgagg taatcctgtt cctgatgttt cacccatagc tacatcatat 1860 cctgtaatta ctgttccttc catgatttca caaataaatc caagcatggg tcttttatct 1920 cctcaagtta atgccatacc taacgtaatg gttcaaagct ccctggattt acctaaacag 1980 ggtgaaaata ataatagttc aaatgttcca gctgcatttg ttccaaagca gttttccacc 2040 aattctcttc cagttcaaaa acctaaatgc cttgctacct taggtttacc aaatggctgg 2100 gaaaaagcta ttagccatga cactgggaga gtttattata aagaccataa tactgaaaca 2160 acccactggg agttgcctat agaagttcaa caacaaatgt taacaaaacc agtaccacaa 2220 aaagaatcaa gtgtttcaaa agcatttaat gattcttttc cagttccaaa caaagtctcc 2280 cataaagatg cagaatcaaa aatgaaaaga tctctctctt cgccaaatct aaatgaagct 2340 agtactgatc aattaccaaa attgaaatcg tttcctgtta tagatcgttt atcgaagcca 2400 acagaaaaaa aaactgtttt agttgataga acaaccaaac ctgtttcagc atcaagatta 2460 gaaaaattgg caccaattta tgggagcatg ggtcgagttt taactggatt aaaaaatctc 2520 ggcaacactt gttatatgaa ctctgtaatt caatgtcttg catgcacatt gccacttgga 2580 aagtatttta caactggaat gtatgagaaa catctgaatt tgtgcaacac taatgcacat 2640 cctggagagc tagcacgtga gttagctttc cttattattg tcatatcagc aggagaatat 2700 cggtctgttt caccaattga cttcaaaatt gctttgggta agtttgcacc tgattttagt 2760 gggtttaaac agcaagatgc tcatgaactt ttgatgtttt ttatggatgg acttcatgaa 2820 gatgtaaaca aaattgtaaa aaaggaaata attaaagtga aagaaactga taatttatca 2880 gacgaaatag ctgctgatat tgcatggaaa gattacaagc taaataatga ttcagtgatt 2940 gttgatctat ttcagggtca atttcgcagc acagtgacat gtttaagttg taataaacgt 3000 tcaaaaactt ttgatgcttt taactgcttg cagctgccac tgccaagtaa tcagtctaca 3060 actctagaga attgtattca aacatttaca aaagctgaaa aaattggtgg gaatgatagt 3120 tggaagtgtt ccaattgcaa gagatatcga gaagcatcta agactattga aatatggaaa 3180 ttacctcctg ttcttattat ccaacttaaa aggtttcact ttgatggtcg ttggcataat 3240 aaaattcaaa ctacagttaa ctttccaatg caaaatttta atatgtccaa atatattgtc 3300 gcgccacaat caatacatac taagtatcat ctttatgcag tgattaacca ttatggaagt 3360 tttgaatcag gtcattatac tgcaaattgt gtaaatcctt attacgaaaa atggtataat 3420 tttgatgata gtattgttaa agagattgat gtaaattcta taaagacgaa tactgcatat 3480 ctcttgtttt acacagcagt agacttcaaa gaatatataa aacgttga 3528 <210> 56 <211> 1012 <212> DNA <213> Hydra magnipapillata <400> 56 atggaaccat ttgatgttgt aaatttttta acttctttaa gttttacttt agatggaact 60 cgttataaac ctttttttga aagaaatcaa agattgtata tgtttatggc agatgccttt 120 aggtcaatat acttgaatta tgaaaaagat gtggagttct atgacatcca actttacagt 180 cacacaacat catttaatct attaggaaat gtgagcatta gtccatggaa tcaagatttt 240 tgtactgatc agtgctatga gacaggagtt ttacatgtcg gaggctgtgc tgattcacca 300 agtgttccag tgtttatgtc atggccgcat ttttatcaag gtgatgaaat gctgtataaa 360 aatgttgatg ggttgagtcc aaataaagaa aaacatgcaa tttttataaa tgtagaaccg 420 attactggaa taactatgca agcaaagatt cgattgcaaa taaatttcct tgtggaaaag 480 attcctttac ttcacatagc agaaggtttg gcagcagaga ggacttatat gccagtgttt 540 ttttttgatg agactggcga aatagatggt aaaaacgcag ctatgttgcg cgagaaggtt 600 attcaattaa gaaaatattt aaaactatcg atagttgtta ccattggaat cggctttatt 660 ctactagtta tttctcagat tctgtttact tttaaaaaaa atacaagttc ttaataatac 720 taatatttat gcctagtcaa tttggacact gatgtcattt taacctcatc taactctatt 780 tcacagtttg actaacgtat ataatacttt ttgcgctatt ttacaattac ttcgaacatc 840 aatcacccca aaatgtatga cattaaacaa tattgtcgaa gctattaatt ttgattttaa 900 aaataatttt taaaaaacat tggtttgaat tttataactt taaattatat ttaagattta 960 atatttatag ttattgaaaa ttatatgaga atttttatac gattattatt aa 1012 <210> 57 <211> 2520 <212> DNA <213> Hydra magnipapillata <400> 57 atgittgact atataaataa tctgaacgag gctcctacgt ctttgtctct ttcttcaact 60 tcattttatg aaagccagct tcctggaact attgtcacat cgatatttat aagtgatcct 120 gatccaaatc aaaaccatac ttgcaagatt ttggatgatg caaatttttt ttcaatgaat 180 ggatcagctg acataaaatt aaccaacaag gtagattacg aaaccatttc atttcttagt 240 attgttgtgg aatgctttga taatggctct cctccattat ctattaagca aaattttact 300 attaatattt tggattccaa tgatcaacca tcagccatca actataatat ttcatcggtt 360 cttgaaaatg ctaattttaa tcaattggta gcccaactca ttgttgaaga tcaagatcag 420 aatcaatcac atttatgcga agctcaagga acaaataaac attggtttta ctttagtaat 480 gaaacaaacg gaaaaatatt cgttttttta atgtcagaga taatgaaact taattatgag 540 acagaaaaaa gtatttcagt cattgtgatt tgtcatgaca atggcacacc accattaagc 600 atccaagata gattcgaatt ctttgtgatt gatgtgaatg aacctgcaac taaaattttc 660 atatcgaatt caagtttcat ttttatgaat gaaaacgtag ttccgggaac aagagttggc 720 ctgttagaat gtgatgatcc agatgttggt caagaaataa aatataattt gcttgaggat 780 tcaaatttga agtttcagat ttatagcggt aatattttgg ttgtaaagaa tctaagcatg 840 tttgattttg aatcatcaga attctctcga gtgattgtaa atatacaggt ttcagataca 900 tcaaaccaac caatagttta caacggaact ttagaaataa ttgctcaaga tgtgaacgag 960 cctccttcta acatcaaata tattgttaat aatttgattt cagatattca ttttattgga 1020 gaaaacagca caattggctc gagcattggt ttgcttttgg ctgataaccc agaaggctcc 1080 gtgcaaatgc ttacgttttc acttttgact tacaaaaatt attttctcat ttcatattac 1140 aacaattctt cttctgatct tgtgttagta aaagagctgg atgttaacga tattagtgat 1200 tttactattt ctgtcaaagt tagtgacaat ggaattccgc ctctttctac tgttggatat 1260 attcgactaa ctgttcttcg atcagatcct tgtgttagtg gtatgatgca ctgtacatcg 1320 aactctgaat gtgctagaaa aaataagaca accggagaat gtttatgtct ccccggattt 1380 gaaatgaatt tgggtgtgtg tcaacaaatt gatgattgca aaccaagttg tcaaaattgt 1440 cttgttagta gcagaaacgc ttgcttaaaa aataatgcac ttgaatgttc accttgtcgt 1500 aacgatggta tatgtactga tcttcatatg aattattcat gcgaatgcaa gaaaggtttc 1560 actggtcttg attgtgcagt cgatattgat gactgcgctc cgaaaccatg tcttcacaat 1620 tccatatgtg aggataaatt taacaattat tcgtgtcact gtgcccctgg atacaaagga 1680 aataattgtg aagaggaaat taatgaatgc gaacaaaatc cttgttataa cggaaattgt 1740 acggatcaaa tcgacggctt tttgtgtact tgtccactgg actattatgg aacaagttgt 1800 cagcgttatt caaagttttg tacgcttgat aaatgttctg aaaactttga gtgtgtgccg 1860 aatccaattt caaaaaaatc agaaataacc tttagatgcg ttccaaagaa tcgaatttta 1920 tcacttttta ttgaagacaa atttgccgta ttgtctcctg acacagcagt ttctttatgg 1980 aagctgcgtt ttgagaattt tttacttgaa aaagttacag ttcctagtcc ctggctagag 2040 tcaaaagttt acgataaata tgttgaatgc aaaataaatg acgcatttat acttaactac 2100 gaatcatgcg ctcaaatgga taaaatgttt ggatgcaaaa tttgtagata tgtcaatgaa 2160 tttcttgcac agaattctgt attgaaaaaa ccagaatggg gaaaatcaaa aagaagtaga 2220 gaagtgaact taaagcttat tatttctgcc ttctcaaaga aaacgagaac taatacgttt 2280 accatggctt cgcgatcaaa ctctatacat ttaactgatg ctgacctaat ggacgggcag 2340 tttaatctca tgtacggtgt ggacgaggag gagctgcaaa gaaacaaaac aataacacca 2400 ggattatatg atttgcttga accagaccaa atattttcga aacagcataa aacagtcaaa 2460 aaaatgtttg ataatccact ctttcatcat aacattaata aagataaaca cataaattaa 2520                                                                         2520 <210> 58 <211> 9312 <212> DNA <213> Hydra magnipapillata <400> 58 atgaaggaag gaatgctttt agagtttgat gatcatctaa ctaaatgtaa tgaacattat 60 actgcacaag aaggaagtat ttcatcacct aactttccac aacactatgg aaataatcgg 120 gattgcactt ggttaattga agttgaaagc tcctatataa tagaaattaa gtttttggaa 180 ttttttattg agaactcttt tgactgggta gaagtatttg atgggccaac tctagcatca 240 aagtcttttg ggaaatttag tggtaatttt aaaccagagg taattttgtc ttcatcatca 300 aaaatcttag ttcatttaaa atctgataaa tcaacaagtg ataaaggatt ttttgcacga 360 tataaagcag tcaaaaaagg ctgcaatgtt gatttaatta taaattcaaa taaaccagaa 420 tatatcagct cacctaactt tccattaaac tatcctgaca atacaaagtg tgaatggaca 480 atcagaaatg aagatccttc aaagattttg acttttcaac tattggaact tgaaactgag 540 gaaaatgatt ttattgaagt tcgtgatggt ttaacagaag ataatgcatt gcttggaaag 600 ttttttggaa acttttcaaa catacaaaaa agacatattt ttacatcgaa ttttaaaatg 660 catgttaagt ttcaatcaaa ttttcaatta aatcaaaaag gattccgagc tgtcattttt 720 tcaggttgca cagcaacaat taatgcaaca catggcacaa tatcgagtcc taattttggc 780 gtcggaaact accccaataa tatactatgc agttggacaga tccatgcaca aactgacaaa 840 gaagtttcag ttatttttca ctcatttgac acagaataca agcaagatac tgtcgtactg 900 agaacatgtg atgatgttat gataaaagaa tgttatggtt ccaataaaac aactttaggg 960 aaagaaatgc ttcctttaga accaattgtt tttaattcat catgttttaa agttattttc 1020 aagtcagatg aaacatattc cctggagggc tttaatgcaa cattttcaat tgggtgtcct 1080 atgcttcctg ctattaatgg gggatctttt aggcaagatc agaaatttta tggtgcttca 1140 gttgaataca aatgtgatag cagattcaga attaaatcag aaaatggaaa tgttcgaaag 1200 tgtatgtatg gtggaaattg gagtgaacct gctccatttt gtgaagaaat aaactgtgga 1260 catcctggaa ctattgagaa tggtggtttt gaaatatatc ctttaaacag tacatttgtt 1320 tttatgactt ctgtcaaata ctcatgtaat gttggttata tacttgttgg cgaaccaaat 1380 cgagtatgtc aagttaactc tacttggtct ggcggagtag aaagaaaatg tgaagtactg 1440 aaatgtcctg atcctggaac tccagttaat ggaacaagat ctggaaatga atttaatgtt 1500 ggatcatcta taactttttc atgtgaaaaa gggtttaggc gtcatggtgc aagaacaatt 1560 acatgtggga ttacaggaaa atggagctct gaaacaccat tgtgtaaaga tgttcctcaa 1620 tgcatagata taaatgaatg tttaaataat ccttgtcgtg gtcctggtag tcagtgcaca 1680 aacttgatgg gaagttataa atgtacatgc aaatctggtt ttagacaaat agatgagaca 1740 acctgtgaag acattgatga gtgcaaatct agtggtaaaa cagttaaatg caatcagaaa 1800 tgtactaata tagatggtgg atatttttgt tcttgtgaag atggttactt tttgtatgat 1860 ggtgacaata aaactgttaa tcgtgttgaa gctgaggagt catttgttaa ccatacttgt 1920 ttaggtaatc catgtaaatt accaaaaatt cctgaaaatg gatatatatt aaactatgga 1980 aaccgttatc caacagagat tacatttcag tgtgataaag gatggatagt tgatggagat 2040 ttatcaatta agtgtttaaa gaatggatca tggtcaggct caatctctga ttgcattgct 2100 gctaggtgta accctttacc tgatgttcca aactcatcaa gtgttgttga tggtggatta 2160 gtaaaaggtc aatattctca aggcactatt tacacataca catgtattaa tggttatgaa 2220 ataatatctg gtaattctaa aagaatgtgc tctcaaaatg ctactgcatc tttttggtct 2280 ggatcatctc ctgtttgtga gcgagtttca tgtggtccat tggaaaatcc taaaaatggt 2340 catgtgaata taactggtga taagtatggt gattcttcta aatttttttg tgatggtggt 2400 tatataatta ctggaaatga ttcaagtact tgtcaagcaa atggcagatg gtcagcatct 2460 tctccgtttt gtctcgcatc accgtgtcct gatcctggca taccaatcaa tggtgaaatt 2520 atcggtttaa tttcattaga tcaaactata acattcagat gtcttgtaaa tggttctatt 2580 ttaattggtg ataataaaag aacttgtatt tttaataaat caactggatt aaatcaatgg 2640 aatggaagtc aaccattttg caaagacaca agtataccta cttttaaatt ttgtcctatg 2700 tcaactattg aatttacatt acctcaatct gaaaaaagta tgatggtcaa ctggacatta 2760 cctactttaa ctgacaatgt tcataactta ctgaatataa cagtttctcc agactatgtt 2820 tcacctatgg ttatgcctgc aggtttgaat ataattactt acacagcaac atataaagat 2880 ggaagcacag cagtttgtga agtaaaaatc aatgttcaag acaaagaaat tccaaagata 2940 ttttgtccta aaaatattgt cattaaaagt gacttatcat caaaggtgtt ttggtctgat 3000 ggtatataca ctgataatgt tggtataaaa aatgttatct ttaacccacc aaatggtaca 3060 atcctagagt ctaataaata ccacaaagtt acaatgacaga tagaagatat ttctgggaac 3120 tctgacagtt gtgtcatgga aatatatgta gaaggttgcg gatgtactca gcgcacttgt 3180 gattacaagc agcggaatgg tcaatcagtg tgtacaattg cacctggggt ggtgcctgat 3240 acagtaatta tttcttgtaa tttaaattgt tttcaaaata aaacaacaag atttgtttcc 3300 aacacagaat ttgaaaaaac aagtaatgtg atgtgtatac ctaataaagg atgggagaac 3360 aagtcaccat caggatgcgt agaaagaaag ccacttattt catgcttaaa aggatcttta 3420 tcaattgact tcacaactac tggggataat acaaatgaaa aaccattttt agatatcttt 3480 acttttttga ataacttgaa gaattcaaca tgttctttta tttatcaaac aggtaactta 3540 atttcaaatg gtacatcagt agctggagat ttcaaaatat tatctcatga tgatggtgtt 3600 attcctttac tgaacggtca tttggaagag tgcaaaacaa ttttacttaa agatcttgac 3660 ctttttaaaa caaatctact tcaattaact tcatctgttg acattaaact tttaaatata 3720 agtagttgct gcccattaaa taaaagttat tgctgtccta ctggacatct aataaaaacc 3780 acaggactaa ctgttaaagc cagctattgc atcccatgtg gcttgggtat gtatcacaat 3840 agcacaaata acaaatgctt aaattgtcct gttggatttt ataatgatct tgaatttgca 3900 gatcaatgta aacgttgtcc taacgaaaca aataatttaa aagttcaatc tgattcatgc 3960 acaaagatgt gtgctgctgg tagctattca tctactggat tagaaccaac atgtcgtaaa 4020 tgtgactatg gtgagtatca atcgcaagtt ggtgcatata attgcattaa gtgtccttat 4080 aaccttacaa catcaagaag tggatcagaa aatatctcag aatgcaaagc ccaatgttct 4140 cctggtactt attcatccac aggtttggag ccttgtacaa aatgtgctaa aggactcttt 4200 caaaatgaaa tagggcgatc atattgcaaa ctgtgtcctt caggaggtat ctatcttggt 4260 atggagggtg caacaagtga agctaattgc ccatcaaaaa atgcatgcga tgaaaagcca 4320 tgtagaaatg gagcgacttg caacgcagga aattactcat acacttgtac ttgtaagcct 4380 ggatggacagta gtgtaaactg tgaagttgat attgatgaat gtgcttcttc tccttgtggt 4440 aggcacggca cttgtacaaa tctcataaac gactttaact gtacctgcat taaaggaata 4500 attggaaaac agtgtgaaac aaacattgat gattgcaaaa atgatacttg tagaaatggt 4560 ggaatttgta atgatcttgt tgatgatttt aaatgtcttt gtgctgctgg ttttgaaggt 4620 aaaagatgcg agataaacaa aaatgaatgt gaaccaaatc cttgcttgaa aaattcaact 4680 tgtgaagatt tgattaatga tttcaaatgc aattgtatac caggttatgt aggcaagtta 4740 tgcgatgttg atattgatga atgtgctatg tctccttgtt ttaacaatgc aacatgtgtt 4800 gataaagtta atgcttttga gtgcaaatgt caacctgggt ttaatggaac actctgtgaa 4860 actgatatag atgagtgtag tactcaacca tgtgcaaata atggctcatg cattgatatt 4920 gttaatggtt tcttgtgcag gtgtatagat ggatttagag gttccaattg ttcaattaac 4980 attgatgagt gtgacccatc tccatgtctt cacaattcaa cctgtgttga tcaaattaat 5040 ggatttcaat gtgaatgttc accaggttat tttggtctaa ggtgtgaaac tgaaataaat 5100 gagtgtgaaa gtcaaccctg ctcaaacaat gctacttgtg tagataagat aaatgattac 5160 caatgtctct gcaatattgg atttgaagga aaacagtgtg aaattgatat aaatgaatgt 5220 caaccaaatc cttgtgttaa tggatcatgc aaagatcttg ttggagatta tttgtgcgaa 5280 tgtcaacctg gttttgatgg aagaaactgt tcaaatctta tagataattg tttttcattg 5340 ccatgcaaaa ataatggaaa ttgtacaaat aaagttaaca attatacttg tacatgtcaa 5400 gcaggtttct caggtagtga ttgtgaaact aatattaatg aatgtgaccc agatccttgc 5460 aatagtaatg ctttaaactg cactgatctc atcaatgggt atatttgtta ttgcaaactt 5520 gggttcagag gggaaaactg ctctgaaata attgataact gtaacccaca accatgcagg 5580 cataattcaa catgtacaaa tcgttttgtt actaatgatt ttcattgtaa ctgcagtctt 5640 ggttatgaag gtgaccgctg tgaaatcgaa attgatgaat gtaaagcttt accttgcaaa 5700 aatggtggaa actgtactga tttgcctggt aaataccttt gtacttgtcc acctggattt 5760 actggagttg attgtgaaat aaacattgat gactgtaaga acgtgagctg tttaaatggc 5820 ggaaaatgta ttgatttaat aaacagctat gtctgcgagt gccctgtaac ccacaaagga 5880 aaacattgtg aaaaaaaaat tccagacgat gttgatatta cctttccggg aggggacggc 5940 tcttacatca agcattgtgt taaaggaaat gcatcaaaag aattttctat atcgctttgg 6000 tttcgatttc ttggaagatc tagaggtgtt tttttgaatc ttggatttgg gtgcgaaact 6060 gttcgtcctt ttcttataat gagccacagt agtatcagat ttcaattccg taaccagttt 6120 agttttgtaa tgattgataa cttgaaaata aacaatgaaa aactagttat taacaatgga 6180 gtttggcatc acatttatat tgcattatca tctgtaacat taaattttaa agttgtgttg 6240 gatggaataa ttgtgcaaga agaacaaaag gcagaattat ctggaatgac ttttttcaat 6300 gagtctggaa caatcttgat tggtaaatct cttttagatg gtaagcttat agaaagtggt 6360 tttattggag atgtaagcca ggtagcatta ttcaaacgtg tgcttgattc aacagagata 6420 gcaggattgg ttagaaattg ttccagaaac atatcagatt caataattcc atgggttggt 6480 ttaatggctg atgctggtcc gaatgttagt ttacttgaaa cttggacttg tggaacaaat 6540 gtatgtcctc ccgagtttac cggaaagttt tgccaaacaa agatagacaa aataccaccc 6600 actgttgttt tttgtccgtc tccaataaaa gtttacactg aggagtcaag tgtttttgta 6660 acttggaaag aacctcaatt tgatgatgat gttgcagtgg ttgaagtgat tagatctcat 6720 tctcccaatg gtttgtttag ttggggagat tatgtaatta cctatattgc aaaagatgct 6780 tttgaaaatt ctgtatcctg tacttttgaa ataaatgtta caccctttaa ttgttcagaa 6840 tttcaaactg ctaataattc cattcatgca tgtggtactt ggaaatatgg tcggttttgt 6900 gattataaat gtcatgagaa atatgagctg gcagttccta agccaccatt ttatgtttgt 6960 ggtagaccag gaacttggag tcatggtccc ccaacatacc cagaaaatcc agatttatta 7020 gtttctgaat gtacagcaac aacacctttc aataccatta ctacaaacaa aatatctttc 7080 ccaacttctt catgcgatga ttcattcaaa aacgattttg tagaaagttt taagcaattg 7140 ataacagaat tgcaagatgt ttggtcaatt tgtggaacaa catgtgattt caaaaatatt 7200 aaagttagct gcaagcctga tgctaaacgc tttcgacgag atatggatcc aaaatggact 7260 ggacccaaat ttatcttgga tatttcattt gatgccacaa atgaaacaaa ccttgctaaa 7320 attcaaaatg gaatcaagac aaaagaattt aatatgactg taatagtgaa caatgtttct 7380 attagttctg atctgttaaa tgttgaagtt aaaacagtat gtaataatgg aagcattttg 7440 aaaaatgaca tctgtgttaa atgtcctgca ggtacattta agaatgttac aaatgaactt 7500 tgtgaaaaat gtccatttgg atcatactca aaagaagctg gatcagaaat gtgtatcaat 7560 tgtccagcta atacaactac aactagtgaa ggttccactg aagagaaatc atgcaaagtg 7620 aaatgtcttt ttggtgcata tcttgaaaat aatgagtgca aagattgtcc aattggattt 7680 tataaagaaa ctgctgggtt cgagaaatgc caaccgtgtg cattaggttt aacaactaaa 7740 aaaacaggag ctgtaacaaa ggatcaatgc aaaaatgatt gcactcttgg tcaagaactg 7800 gttaacaata catgtcaacc ctgccgtatg ggttactata gaaataatga tagtcaacct 7860 gtatgcttac cttgtcctta caaccaatca acatatcaac taggagcaac tagtcctgtt 7920 tgctatgctg tttgtgaagt tggaaaagag atatcaagtg atggattgaa atgtatggag 7980 tgtgctatag gttattataa aaatgacaat aacgcaaaaa cttgttcaaa gtgtcctgca 8040 gggttaacca ctaaacagtt agcttctaca agcattgacc aatgtataga aaagtcatct 8100 gactcaaaat attatgatgt ccaagtagca attacatcac taacatggag tgataatctt 8160 tataaccaaa attcagttga gtacagagat gtagcatata aaataattga atcagtttat 8220 ggtgtgtact tgaatatgag ttatttgcta tttgtgaaca ttactaaact aagttctggt 8280 agtgttgttg caaacttaac attaggattt aactcatctg ttaaagatcc actttttgct 8340 ttcgattctg cagttaaatc acaacaattt tacaaactta ctgttgatcc agatagttta 8400 attgcagatc gttgtaaaca cattgtttgc ccaatggaac aagtatgcag ttatgatact 8460 cgagttgagt gcaagtgtac agttggaaaa caaaagagtc ctgatggtga aaaatgttat 8520 gatacttgtg agcttaaagg gtgtcctgaa ggacaggaat gtttttcaaa ttcaacattt 8580 aatgagtgca gatgtaaaga tggaaaagtt cttagttctg acaaaaaaga atgtatctat 8640 acttgtgagc tcaaagggtg tcctgaagga caggagtgtt tttcaaattc aacatttaat 8700 gagtgcagat gcaaagatgg aaaagttctt agtcctgaca aaaaaagatg tatcaatcaa 8760 tgctccttgc attattgcaa aaatggtggt agttgtcagt taaaagaaac aggaccagta 8820 tgcagatgtt caaagaatta ttctggagat caatgtcaaa cactacaaac taaatccaat 8880 gt; gtgcttaaaa atcgcaaaaa gcgaatatat aatgaatgta gaaatgattt aatgtatgat 9000 gatto ttaactcgat acgatcattt gcgttcttca gaaaaactag ggagaataaa tggaggtgca 9120 acgtttgatg ataattctcc tgcaccttta atcaggaatt ctgaccaaaa gcgataacgt 9180 agtcataaaa agctagatgt atccatagaa acgtagccat agaaagcttg ggagaaaaac 9240 aatcaacgga caatgtttat atatatattt tttttgttat taatatttga aagatgcttt 9300 agttttgttt tt 9312 <210> 59 <211> 588 <212> DNA <213> Hydra magnipapillata <400> 59 atattgatga atgtgtcttg tcagcaacaa acaactgctc agttaatgct atatgtaaca 60 attttgctgg tggatacaac tgtgtatgta aaagtggcta taaaacaaat gatggtggtg 120 tagaatcact tactaatcaa tgtcaagata ttgatgaatg tgctttatca actacaaaca 180 tctgttcagt taatgctata tgtaacaatt ttgctggcgg atacaactgt gtatgtaaaa 240 gtggctatca aacaaataat ggtggagtag aatcactcac taatcaatgt caagatattg 300 atgaatgtgc tttatcaact acaaatatct gttcaattaa tgctatatgt aacaattttg 360 ctggtggata taactgtgta tgtaaaagtg gctataaaac aattgatggt ggagtagaat 420 cactcattaa tgaatgtcaa gatattaatg aatgtgcttt atcaactacg aacgcctgtt 480 caattaatgc tatatgtaac aattttgctg gtggatacaa ctgtgtatgt aaaattggct 540 ataaaacaag tgatggtgga gtagaatcgc tcactaatca atgtcaag 588 <210> 60 <211> 1058 <212> DNA <213> Hydra magnipapillata <400> 60 atgttagcaa tagttcattt tattatccat aaaaagcagt ttgttttaaa tcaaggttta 60 attcttggaa cactcgatgt tttaaggaag gaacacacta tcatttttaa ggtttatcct 120 aaaagttact ctaaaggctg gaagagtgtt tttcatttaa cccagggtaa agattacgga 180 gcttatggtg acagaattcc aggtgtttgg tttcatgatg acggctccgg aaaactttca 240 atttttactg cagttaacgg taacaataac tattacgttg caacagctcc gcttccatta 300 aatcaatggt ccagtgttaa aatatgtcaa agtgtacata acgggaagta ttggtattct 360 gttgatctta atggaatcaa cattcataga gttgaaaatt ctgacgccag agattttcaa 420 aacataatag tatacgcctc tgacccttgg tatgctgctc aaaacgctct aatttctaat 480 attatcattg tcaatggtaa cattgaaact attattggca gcgtacctgc aaatttagtc 540 aaaggaaaac ttattgcaga aatacccaag ttagataagg aatatttggt ttcgtttgat 600 atctatccta ataagtttgt tgctggttgg catagtgtta ttcatttctc tattggttct 660 gattatggtc gttatggcga tcgaactcct ggtatctggt ttcacactga cggaaaagga 720 ggtctctaca tttcagcgcc aattaatggt aatgtcgaca gagtttttac cacaaatccg 780 attggattaa atcattggtc tagtgttgaa gttagtcaat ttttaagaaa ctcagtttac 840 atatacacaa tcagaataaa tgaagaaatt gtcttttctg aaataaataa cctggctcaa 900 agttttgata atgttaaagt atatgcatca gatccttggt atgatgctca atatggctca 960 ataagaaatt tatttgttgt caatggtgtt tccaataatg gactccaggc agatattgtt 1020 ttacctacag gtatataaaa tattatttga tatttttt 1058 <210> 61 <211> 967 <212> DNA <213> Hydra magnipapillata <400> 61 atgtttggag tggggagtat tgctcaagtt gtccttggaa aaggaaaatt tggaggaata 60 ttatcaatca acttcggctg ggcaattggt gtaatgtttg gggtctattg gtctgctggt 120 gtttcaggtg gtcatttgaa tcctgctgtt acactagcaa tggccgttac taaacgatta 180 ccttggaaaa agttgcctgt gtattggatt ggccaaagtc ttggagcatt tattgcctcg 240 gcagttattt acggagttta ctacgatatg ctaaacgaat atgacggagg ggttagaaaa 300 gtcgatacgg cgtggatatg gacaactcgt ccttcttctg gtgtctcaac cgtaactacg 360 tttgtcgacc agtttgttag tagtggactt cttgtcggta ttatatttgc tttgacagat 420 aaacgtaata acgcaccaag ccctcgagtg ttgccattgt gtgttggaat agtcgtactt 480 ggtattggta tttcgtttgg tcttaattgc ggatatggta ttaacccagc tcgtaatctt 540 attccaaggc tttttacatc gattgcaggg tggaaatcag caccactaag ctaccaaaat 600 tattattttt gggcgcctat cttggctcaa ctagttggag gtgtttcagg cgcccttttg 660 tatattgtaa caattgaaat gcatcacaac agtaaaaaag aggggcaagt tattttaaaa 720 gaacaaaaaa atagactaac tgaacaaccg gtttaaagat ttatgacaat tgacaaaaaa 780 tatattttct tgaaagattc cctgttcttt tttttagttg ttagtatttt gtaattttta 840 ttttatatct tttcaactat atttgaaaaa acctgtaaat tttgtttata atcttttttt 900 attataatca cgttaacatc atgtttagca aatttgatgc tacagtgtat gcagtttgta 960 aaagcac 967 <210> 62 <211> 936 <212> DNA <213> Hydra magnipapillata <400> 62 gcaacacctt cagtttagca gctgtcgata taacaataaa ttaatttatc aaaaatgcac 60 gtttttaaag atgtttttac gcacgaggat gtgttttcag atgctttcac gtacgatacg 120 gatgataccg atttgtttta tatcgtgcaa ggaaaaataa tttcagagca tgaaggaata 180 gacgacaaac tcattagtgc aaacaaatct tttctaaaaa aaggtgaaaa gttacacgac 240 aaagacttgc tctttcctga cattatccgt gcaaaccagc ttgatcgaat aaaatctatt 300 catagtaaag aacgcctcaa aaagttactg gatgcttatg tagctaagct aacgaatttt 360 gttaaagatg aaaaacgatt gtctgttata aaagaaaaca tgaataaaac tttagacaat 420 ttatttattt ctcgctatag cgataaatta agtttttatg aaacagaagg aaatgcagta 480 gatatgcaag gaatgattat tgtctacgaa caagatgatg ttcatggatc agaagttgtc 540 ggacatggtt gcaagatgta tgtgttcaaa gatggagttt acaaagtcga cgtttgaata 600 aaaactaatt tttaaactct ggttgctttg gcaatcataa aaaagtaaac tactaaatgt 660 tatagagaaa taatatttat gaaaatatca gacaaataga tgtataatgt aagatgtaat 720 tatgagcagc ctaatttctg cttgttagat ttgatttgtt gttaaacatc tggttttatt 780 tgtcatttat tatattagat taaattttta tttgtatcaa ttatatttgg taatggcatt 840 cttttatttt ttgacattta ttgaatattt tatgcatctt atttatgtaa aatatcttga 900 aaaattctta actcttgagt tgagttgtat taaaaa 936 <210> 63 <211> 956 <212> DNA <213> Hydra magnipapillata <400> 63 ctctgataaa atacagccaa aaactttttt tttttaaaga aatgaatata attatgaaaa 60 tttttatcag catcattagt atccaaaaaa tatatggcac tgctgatttc agccaaagta 120 ttaaacagta ctggagcgca gagctttttg ccatgctaaa taatcagcct gaattgcttg 180 gaatcacttg gaaagataaa aaaatggtcg tttcatcaaa cagattggtt cagctgtttt 240 cgagcaaaaa caaaaaagtt ctccaggttc aaaaagattc agtcaatgca aaaggatctg 300 gtagttctat ttattccaat ctgagcattg aaaccgttag ctacaatttg gttcgaatca 360 gaggagttaa agctaaccgt tacatttgtt ttaattttac aagtaaaaca cttattgctc 420 agaaaaatgg caacaacatg gactgtgtat ttgaggaatt gcatagtgaa aaatcttcta 480 gaacatactt taaggcagta acaaaaaaat ggtatattgc aattaagaaa aatggaaatc 540 ttagaccagg ctatttatgg gaagaaaaaa aattcgattc ccctccaaaa actatggagt 600 ttgttgcaag gacttatatg tgataccagc taaaaaacaa aaatcaaaat aaaacataag 660 aaacaatccg cagctgaaag tagtaagagc ccaccaacaa acgaataaag caaaaaatca 720 agttgaaaaa ggaaatgtgg acaacatttc aaactaaaca aattatatcc ttattttttc 780 caaagtattt gaagtttttg ataggtgctt aacattaatg gatataatgt atatttaaac 840 acttttatta aatgttttga ggaatgctat ggcgattaac tcgataataa aaagtctatg 900 tttaaaactg aaactcaatc gaaatgaatt gttactgaaa agtttatttg aaaaat 956 <210> 64 <211> 679 <212> DNA <213> Hydra magnipapillata <400> 64 acatagttag gcttctacag tgttagaacc agcgcagtgt tataaaactt tacatcttaa 60 actttaatac attatccaaa cataaaaagc atggcttctg gacaattaca tcaagaaagc 120 gaagatgcta ttaacaacca aataaacatg gagttgtatg caagttacca atatctttct 180 atggcctatt attttgatca agatgatgtt gcattagacg gatacttcaa gtttttcaag 240 catcaatcag atgaagaacg tgaacatgct caggaactaa tggactacca aaataaacgt 300 ggtgggcgtg tagtttacaa ggatattcaa gctcccaaat ttcaactgga tacgccagtt 360 tcagctttag aagcagcgct taatttggag aagaaagtta acgagtcgtt gttaaatgtt 420 catgccatcg ctgaaaaaaa cagtgatcct catttgtgcg acttcttaga atcagagttc 480 ttgaacgaac aagttgagtc aatcaatgag attgccaagt tgattactaa cgctaagaga 540 tgcggcgatg gtttgggtgt ctatcaattt gataagttga gcatgtcaag ttaaatgtca 600 atgttttttt atttcattgt tttgccttga ggactataca gtgtaaacaa tgttttttgt 660 taaagaaaaa taaatatta 679 <210> 65 <211> 696 <212> DNA <213> Hydra magnipapillata <400> 65 cacactgata tcatattgat cacaacaatg ccaaataatg atccaatgta tagtgcctta 60 caaatcaata ttccaccaga attgcctgaa atactaaaac agtttacaaa agctgctatt 120 cttacccaac ctaaagatct tttacagtgg tctgcagcat attttcttgc actttctaaa 180 ggtgaaagac ctcctgttaa agaacggctt gattttcagc tgggtcaagt tagtgaaaaa 240 acacttacca aagaagcaat tattgttcta cataggcagt taggacataa acctatagtt 300 gaactttcaa aggttgaaga aaaatggaat gcactttgtt tatcggatga agatctgaaa 360 aatgttttgc gcgtgggtag ttttgcagag gagtttgaat ggttgaaatt tcttgcgtta 420 tcatgtagtc atgtatctaa atcaatcaca gaggcgttaa cgttaatttg tgaagtcatt 480 acaaaggatc tggagggtgg gagagcaaga atagaatttg aaactttcaa atatctttat 540 aaatatcttg cagttattga tggagacata ccactatcac atgtgactaa ggtcttagaa 600 caccttgatt ttgatgtcaa gcagcaaagt ggtatgattg ctcctcgtaa ttttaccagt 660 agttcttgtc ctctcttata cacagagaaa ccttaa 696 <210> 66 <211> 1377 <212> DNA <213> Hydra magnipapillata <400> 66 atgccaaatg aagatccatc tactaactct gatcctcagc caggctcatc taaagcgcaa 60 gttcaaggtt ctgaggaact tgatgaactc acagtaaata ttttagaaaa gatttctgaa 120 atcgaatatc tttttttggt tatagacatt aacgaatgtg cattgtcaac gaaaaacaac 180 tgctctgcta atgccatatg taataatttg gttggtggat acaactgcac atgtaaaagt 240 gggttcaata caagtaacgg aggaatagaa tctctttccg atcagtgtct ggatattaat 300 gaatgtgttt tttcaaacaa aaacaattgc tcatctaatg caacatgtaa caatctgaca 360 tcaatgaatg tgccttgtcc aacaagaaca actgctcagt taacgcaacc tgtaacaatt 420 tggttggtgg atacaactgc acgtatttct gaaatcgaat atcttttttt ggttatagac 480 attaacgaat gtgcattgtc aacgaaaaac aactgctctg ctaatgccat atgtaataat 540 ttggttggtg gatacaactg cacatgtaaa agtgggttca atacaagtaa cggaggaata 600 gaatctcttt ccgatcagtg tctggatatt aatgaatgtg ttttttcaaa caaaaacaat 660 tgctcatcta atgcaacatg taacaatctg acatcaatga atgtgccttg tccaacaaga 720 acaactgctc agttaacgca acctgtaaca atttggttgg tggatacaac tgcacgtatt 780 tctgaaatcg aatatctttt tttggttata gacattaacg aatgtgcatt gtcaacgaaa 840 aacaactgct ctgctaatgc catatgtaat aatttggttg gtggatacaa ctgcacatgt 900 aaaagtgggt tcaatacaag taacggagga atagaatctc tttccgatca gtgtctggat 960 attaatgaat gtgttttttc aaacaaaaac aattgctcat ctaatgcaac atgtaacaat 1020 ctgacatcaa tgaatgtgcc ttgtccaaca agaacaactg ctcagttaac gcaacctgta 1080 acaatttggt tggtggatac aactgcacgt atttctgaaa tcgaatatct ttttttggtt 1140 atagacatta acgaatgtgc attgtcaacg aaaaacaact gctctgctaa tgccatatgt 1200 aataatttgg ttggtggata caactgcaca tgtaaaagtg ggttcaatac aagtaacgga 1260 ggaatagaat ctctttccga tcagtgtctg gatattaatg aatgtgtttt ttcaaacaaa 1320 aacaattgct catctaatgc aacatgtaac aatctggttg gtgggtacaa ggcacat 1377

Claims (13)

A microarray for confirming exposure to benzopyran, in which the oligonucleotide of the nucleic acid sequence of any one or more genes selected from the following group or its complementary strand molecule is directly introduced:
(Heat shock protein 90, alpha), the gene described in SEQ ID NO: 3 (Heat shock protein 70), the gene described in SEQ ID NO: 4 (SEQ ID NO: (Small heat shock protein), a gene described in SEQ ID NO: 6 (Calpain-5), a gene described in SEQ ID NO: 7 (MAtrix metalloproteinase), and SEQ ID NO: 8 (Caspase 7), the gene described in SEQ ID NO: 10 (Eukaryotic translation initiation factor 4 gamma, 3), the gene described in SEQ ID NO: 11 (Tetraspanin 33), the sequence (NADH dehydrogenase 1 alpha subcomplex 4), the gene described in SEQ ID NO: 13 (Complementation group C), the gene described in SEQ ID NO: 14 (Cathepsin L-like protease) Genetically listed (Ikappa B kinase gamma), a vascular endothelial growth factor, a gene described in SEQ ID NO: 17 (Noggin 1), a gene described in SEQ ID NO: 18 (Oxidative stress protein) A gene described in SEQ ID NO: 20 (Sox17 alpha), a gene described in SEQ ID NO: 21 (SRY-box containing gene 32), a gene described in SEQ ID NO: 22 (Homeobox protein Otx) (Calmodulin), a gene described in SEQ ID NO: 24 (Potassium channel homolog), a gene described in SEQ ID NO: 25 (Hydra Na channel 3), a gene described in SEQ ID NO: 26 (Caspase 3 preproprotein), a gene described in SEQ ID NO: 28 (Mediator of DNA damage checkpoint 1), a gene described in SEQ ID NO: 29 (stress-induced-phosphoprotein 1 (Hsp70 / Hsp90-organizing pr (Neogenin), the gene (Pellino homolog 1) described in SEQ ID NO: 31, the complementary component C3-like protein (SEQ ID NO: 32) A gene described in SEQ ID NO: 34 (Forkhead box-containing transcription factor FoxQ2b), a gene described in SEQ ID NO: 35 (Homeodomain protein Otx), SEQ ID NO: 36 (Hym-176 preprohormone), the gene described in SEQ ID NO: 37 (Hym-355 preprohormone), the gene described in SEQ ID NO: 38 (Calcium transporter 2), the gene described in SEQ ID NO: 39 (Calmodulin CG8472- A gene described in SEQ ID NO: 41 (Hydra Na channel 4 (hynac4)), a gene described in SEQ ID NO: 42 (Hydra Na channel 2), a gene described in SEQ ID NO: SEQ ID NO: 43 (Calcium channel, voltage-dependent, alpha2 / delta subunit 1), a gene described in SEQ ID NO: 44 (Goosecoid), a gene described in SEQ ID NO: 45 (Cholinergic receptor, nicotinic, alpha 9) (TNF-receptor-associated factor 1), a gene described in SEQ ID NO: 49 (Rho (SEQ ID NO: 49)), a gene described in SEQ ID NO: GTPase activating protein 6), a gene described in SEQ ID NO: 50 (Neuronal acetylcholine receptor subunit alpha-7), a gene described in SEQ ID NO: 51 (Periculin), a gene described in SEQ ID NO: 52 (Senataxin) A gene described in SEQ ID NO: 54 (DnaJ subfamily A member 2), a gene described in SEQ ID NO: 55 (Ubiquitin specific peptidase 8), a gene described in SEQ ID NO: 56 (Cd36 / Scavenger rec (FAT tumor suppressor homolog 4), the gene (Notch) described in SEQ ID NO: 58, the gene described in SEQ ID NO: 59 (Novel EGF domain containing protein 61, a gene described in SEQ ID NO: 61 (Aquaporin 9a), a gene described in SEQ ID NO: 62 (Translationally controlled tumor protein), a gene described in SEQ ID NO: 63 (Fibroblast growth factor 24, a gene described in SEQ ID NO: 64 (Ferritin), a gene described in SEQ ID NO: 65 (Ropporin 1 -like), and a gene described in SEQ ID NO: 66 (EGF-like module containing mucin- .
The method of claim 1, wherein the gene is hydra (Hydra magnipalillata ). &lt; / RTI &gt;
(Heat shock protein 90, alpha), a gene described in SEQ ID NO: 3 (Heat shock protein 70), a gene represented by SEQ ID NO: The gene described in SEQ ID NO: 4 (Glutathione S-transferase), the gene described in SEQ ID NO: 5 (Small heat shock protein), the gene described in SEQ ID NO: 6 (Calpain- (Caspase 7), the gene described in SEQ ID NO: 10 (Eukaryotic translation initiation factor 4 gamma, 3), and the gene described in SEQ ID NO: 11 Wherein the gene (Tetraspanin 33) is a gene whose expression level is increased or decreased by 4 hours exposure to benzopyran compared with the normal control.
(Heat shock protein 90, alpha), a gene described in SEQ ID NO: 3 (Heat shock protein 70), a gene described in SEQ ID NO: 4 (Glutathione S-transferase) (Small heat shock protein), a gene described in SEQ ID NO: 8 (Ks1 protein), a gene represented by SEQ ID NO: 9 (Caspase 7), a gene represented by SEQ ID NO: 12 (NADH dehydrogenase ubiquinone 1 alpha subcomplex 4, Complementation group C, Cathepsin L-like protease, IkappaB kinase gamma, SEQ ID NO: 15, A gene described in SEQ ID NO: 17 (Noggin 1), a gene described in SEQ ID NO: 18 (Oxidative stress protein), a gene described in SEQ ID NO: 19 (SNF1-like kinase 2), SEQ ID NO: 20 A gene described in SEQ ID NO: 22 (Homeobox protein Otx), a gene described in SEQ ID NO: 23 (Calmodulin), a gene (SEQ ID NO: A gene described in SEQ ID NO: 24 (Potassium channel homolog) and a gene described in SEQ ID NO: 25 (Hydra Na channel 3) are genes whose expression levels are increased or decreased by 12 hours exposure to benzopyran compared with the normal control group Microarray for confirmation of benzoprene exposure.
(Heat shock protein 90, alpha), a gene described in SEQ ID NO: 3 (Heat shock protein 70), a gene described in SEQ ID NO: 4 (Glutathione S-transferase) (Caspase 7), the gene described in SEQ ID NO: 12 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4), the gene described in SEQ ID NO: 15 (IkappaB kinase gamma), the SNF1-like kinase 2, the gene described in SEQ ID NO: 20 (Sox17 alpha), the gene described in SEQ ID NO: 21 (SRY-box containing gene 32 A gene described in SEQ ID NO: 24 (Potassium channel homolog), a gene described in SEQ ID NO: 26 (Vasa-related protein CnVAS1), a gene described in SEQ ID NO: 27 (Caspase 3 preproprotein) (Mediator of DNA damage checkpo (Stress-induced-phosphoprotein 1 (Hsp70 / Hsp90-organizing protein)), the gene described in SEQ ID NO: 30 (Neogenin), the gene described in SEQ ID NO: 31 (Pellino homolog 1 33, a gene described in SEQ ID NO: 33 (DnaJ (Hsp40) homolog, subfamily C, member 3), a gene described in SEQ ID NO: 34 (Forkhead box- (Homeodomain protein Otx), the gene described in SEQ ID NO: 36 (Hym-176 preprohormone), the gene described in SEQ ID NO: 37 (Hym-355 preprohormone), SEQ ID NO: 38 (Calmodulin CG8472-PA), a gene described in SEQ ID NO: 40 (Potassium voltage-gated channel subfamily A member 1), a gene described in SEQ ID NO: 41 (Hydra Na channel 4 (hyn a gene described in SEQ ID NO: 42 (Hydra Na channel 2), a gene described in SEQ ID NO: 43 (calcium channel, voltage-dependent, alpha2 / delta subunit 1) A gene described in SEQ ID NO: 45 (Cholinergic receptor, nicotinic, alpha 9), a gene described in SEQ ID NO: 46, an epidermal growth factor receptor, 48, a gene described in SEQ ID NO: 49 (Rho GTPase activating protein 6), a gene described in SEQ ID NO: 50 (Neuronal acetylcholine receptor subunit alpha-7) (Periculin) is a gene whose expression level is increased or decreased by 24 hours exposure to benzopyran as compared to a normal control.
(Heat shock protein 90, alpha), a gene described in SEQ ID NO: 3 (Heat shock protein 70), a gene represented by SEQ ID NO: (Caspase 7), the gene described in SEQ ID NO: 12 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4), the gene described in SEQ ID NO: 15 (Ikappa B kinase gamma), a vascular endothelial growth factor, a gene described in SEQ ID NO: 20 (Sox17 alpha), a gene described in SEQ ID NO: 23 (Calmodulin) A gene described in SEQ ID NO: 30, a gene described in SEQ ID NO: 36 (Hym-176 preprohormone), a gene represented by SEQ ID NO: Logger A gene described in SEQ ID NO: 43 (calcium channel, voltage-dependent, alpha2 / delta subunit 1), a gene described in SEQ ID NO: 45 (Cholinergic receptor, nicotinic, alpha 9) (TNF-receptor-associated factor 1), a gene described in SEQ ID NO: 51 (Periculin), a gene described in SEQ ID NO: 52 A gene described in SEQ ID NO: 53, a gene described in SEQ ID NO: 54 (DnaJ subfamily A member 2), a gene described in SEQ ID NO: 55 (Ubiquitin specific peptidase 8) (FAT tumor suppressor homolog 4), a gene (Notch) described in SEQ ID NO: 58, a gene described in SEQ ID NO: 59, a gene described in SEQ ID NO: (Novel EGF domai (Tyrosine kinase receptor), a gene described in SEQ ID NO: 61 (Aquaporin 9a), a gene described in SEQ ID NO: 62 (Translationally controlled tumor protein), a gene described in SEQ ID NO: (Fibroblast growth factor 24), a gene described in SEQ ID NO: 64 (Ferritin), a gene described in SEQ ID NO: 65 (Ropporin 1-like), and a gene described in SEQ ID NO: 66 sequence 4) is a gene whose expression level is increased or decreased compared to the normal control by 48 hours exposure to benzopyran.
1) separating the RNA from the experimental group hydra and the control group, hydra, respectively;
2) labeling the experimental group and the control group with different fluorescent substances while synthesizing cDNA from the RNA of the experimental group and the control group of step 1);
3) hybridizing the cDNA labeled with the different fluorescent material of step 2) with the microarray of claim 1;
4) analyzing the reacted microarray; And
5) confirming the degree of expression of the gene integrated in the microarray of claim 1 in comparison with the control group in the analyzed data.
The method according to claim 7, wherein the fluorescent material of step 2) is selected from the group consisting of Cy3, Cy5, poly L-lysine-fluorescein isothiocyanate (RITC), rhodamine-B-isothiocyanate (RITC), and rhodamine Wherein the method comprises the steps of:
1) separating the RNA from the hydra of the test group exposed to the test sample and the hydra of the normal control group, respectively;
2) Performing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) using primer pair complementary to each of the following genes and amplifying the gene, step; And
(Heat shock protein 90, alpha), the gene described in SEQ ID NO: 3 (Heat shock protein 70), the gene described in SEQ ID NO: 4 (SEQ ID NO: (Small heat shock protein), a gene described in SEQ ID NO: 6 (Calpain-5), a gene described in SEQ ID NO: 7 (MAtrix metalloproteinase), and SEQ ID NO: 8 (Caspase 7), the gene described in SEQ ID NO: 10 (Eukaryotic translation initiation factor 4 gamma, 3), the gene described in SEQ ID NO: 11 (Tetraspanin 33), the sequence (NADH dehydrogenase 1 alpha subcomplex 4), the gene described in SEQ ID NO: 13 (Complementation group C), the gene described in SEQ ID NO: 14 (Cathepsin L-like protease) Genetically listed (Ikappa B kinase gamma), a vascular endothelial growth factor, a gene described in SEQ ID NO: 17 (Noggin 1), a gene described in SEQ ID NO: 18 (Oxidative stress protein) A gene described in SEQ ID NO: 20 (Sox17 alpha), a gene described in SEQ ID NO: 21 (SRY-box containing gene 32), a gene described in SEQ ID NO: 22 (Homeobox protein Otx) (Calmodulin), a gene described in SEQ ID NO: 24 (Potassium channel homolog), a gene described in SEQ ID NO: 25 (Hydra Na channel 3), a gene described in SEQ ID NO: 26 (Caspase 3 preproprotein), a gene described in SEQ ID NO: 28 (Mediator of DNA damage checkpoint 1), a gene described in SEQ ID NO: 29 (stress-induced-phosphoprotein 1 (Hsp70 / Hsp90-organizing pr (Neogenin), the gene (Pellino homolog 1) described in SEQ ID NO: 31, the complementary component C3-like protein (SEQ ID NO: 32) A gene described in SEQ ID NO: 34 (Forkhead box-containing transcription factor FoxQ2b), a gene described in SEQ ID NO: 35 (Homeodomain protein Otx), SEQ ID NO: 36 (Hym-176 preprohormone), the gene described in SEQ ID NO: 37 (Hym-355 preprohormone), the gene described in SEQ ID NO: 38 (Calcium transporter 2), the gene described in SEQ ID NO: 39 (Calmodulin CG8472- A gene described in SEQ ID NO: 41 (Hydra Na channel 4 (hynac4)), a gene described in SEQ ID NO: 42 (Hydra Na channel 2), a gene described in SEQ ID NO: SEQ ID NO: 43 (Calcium channel, voltage-dependent, alpha2 / delta subunit 1), a gene described in SEQ ID NO: 44 (Goosecoid), a gene described in SEQ ID NO: 45 (Cholinergic receptor, nicotinic, alpha 9) (TNF-receptor-associated factor 1), a gene described in SEQ ID NO: 49 (Rho (SEQ ID NO: 49)), a gene described in SEQ ID NO: GTPase activating protein 6), a gene described in SEQ ID NO: 50 (Neuronal acetylcholine receptor subunit alpha-7), a gene described in SEQ ID NO: 51 (Periculin), a gene described in SEQ ID NO: 52 (Senataxin) A gene described in SEQ ID NO: 54 (DnaJ subfamily A member 2), a gene described in SEQ ID NO: 55 (Ubiquitin specific peptidase 8), a gene described in SEQ ID NO: 56 (Cd36 / Scavenger rec (FAT tumor suppressor homolog 4), the gene (Notch) described in SEQ ID NO: 58, the gene described in SEQ ID NO: 59 (Novel EGF domain containing protein 61, a gene described in SEQ ID NO: 61 (Aquaporin 9a), a gene described in SEQ ID NO: 62 (Translationally controlled tumor protein), a gene described in SEQ ID NO: 63 (Fibroblast growth factor 24, a gene described in SEQ ID NO: 64 (Ferritin), a gene described in SEQ ID NO: 65 (Ropporin 1 -like), and a gene described in SEQ ID NO: 66 (EGF-like module containing mucin-
3) comparing the gene product of step 2) with a control, and confirming the degree of expression.
A kit for confirming exposure to benzo [a] pyrene (BaP) comprising the microarray of claim 1.
The method according to claim 9, further comprising the step of adding any one selected from the group consisting of a fluorescent substance consisting of a strepavidin-like phosphatease conjugate, a chemiluminescent substance, and a chemiluminescent substance Wherein the kit comprises:
10. The method according to claim 9, which is selected from the group of reaction reagents consisting of the buffer solution used for hybridization, the reverse transcriptase for the synthesis of cDNA from RNA, dNTPs and rNTPs (premixed or separated feed), labeled reagents, and wash buffer solution Wherein the method further comprises the step of detecting the presence of benzopyran.
Kit for confirming exposure to benzo [a] pyrene (BaP) containing a primer pair complementary to each of the following genes and capable of amplifying the gene:
(Heat shock protein 90, alpha), the gene described in SEQ ID NO: 3 (Heat shock protein 70), the gene described in SEQ ID NO: 4 (SEQ ID NO: (Small heat shock protein), a gene described in SEQ ID NO: 6 (Calpain-5), a gene described in SEQ ID NO: 7 (MAtrix metalloproteinase), and SEQ ID NO: 8 (Caspase 7), the gene described in SEQ ID NO: 10 (Eukaryotic translation initiation factor 4 gamma, 3), the gene described in SEQ ID NO: 11 (Tetraspanin 33), the sequence (NADH dehydrogenase 1 alpha subcomplex 4), the gene described in SEQ ID NO: 13 (Complementation group C), the gene described in SEQ ID NO: 14 (Cathepsin L-like protease) Genetically listed (Ikappa B kinase gamma), a vascular endothelial growth factor, a gene described in SEQ ID NO: 17 (Noggin 1), a gene described in SEQ ID NO: 18 (Oxidative stress protein) A gene described in SEQ ID NO: 20 (Sox17 alpha), a gene described in SEQ ID NO: 21 (SRY-box containing gene 32), a gene described in SEQ ID NO: 22 (Homeobox protein Otx) (Calmodulin), a gene described in SEQ ID NO: 24 (Potassium channel homolog), a gene described in SEQ ID NO: 25 (Hydra Na channel 3), a gene described in SEQ ID NO: 26 (Caspase 3 preproprotein), a gene described in SEQ ID NO: 28 (Mediator of DNA damage checkpoint 1), a gene described in SEQ ID NO: 29 (stress-induced-phosphoprotein 1 (Hsp70 / Hsp90-organizing pr (Neogenin), the gene (Pellino homolog 1) described in SEQ ID NO: 31, the complementary component C3-like protein (SEQ ID NO: 32) A gene described in SEQ ID NO: 34 (Forkhead box-containing transcription factor FoxQ2b), a gene described in SEQ ID NO: 35 (Homeodomain protein Otx), SEQ ID NO: 36 (Hym-176 preprohormone), the gene described in SEQ ID NO: 37 (Hym-355 preprohormone), the gene described in SEQ ID NO: 38 (Calcium transporter 2), the gene described in SEQ ID NO: 39 (Calmodulin CG8472- A gene described in SEQ ID NO: 41 (Hydra Na channel 4 (hynac4)), a gene described in SEQ ID NO: 42 (Hydra Na channel 2), a gene described in SEQ ID NO: SEQ ID NO: 43 (Calcium channel, voltage-dependent, alpha2 / delta subunit 1), a gene described in SEQ ID NO: 44 (Goosecoid), a gene described in SEQ ID NO: 45 (Cholinergic receptor, nicotinic, alpha 9) (TNF-receptor-associated factor 1), a gene described in SEQ ID NO: 49 (Rho (SEQ ID NO: 49)), a gene described in SEQ ID NO: GTPase activating protein 6), a gene described in SEQ ID NO: 50 (Neuronal acetylcholine receptor subunit alpha-7), a gene described in SEQ ID NO: 51 (Periculin), a gene described in SEQ ID NO: 52 (Senataxin) A gene described in SEQ ID NO: 54 (DnaJ subfamily A member 2), a gene described in SEQ ID NO: 55 (Ubiquitin specific peptidase 8), a gene described in SEQ ID NO: 56 (Cd36 / Scavenger rec (FAT tumor suppressor homolog 4), the gene (Notch) described in SEQ ID NO: 58, the gene described in SEQ ID NO: 59 (Novel EGF domain containing protein 61, a gene described in SEQ ID NO: 61 (Aquaporin 9a), a gene described in SEQ ID NO: 62 (Translationally controlled tumor protein), a gene described in SEQ ID NO: 63 (Fibroblast growth factor 24, a gene described in SEQ ID NO: 64 (Ferritin), a gene described in SEQ ID NO: 65 (Ropporin 1 -like), and a gene described in SEQ ID NO: 66 (EGF-like module containing mucin- .

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