KR20140109631A - Composition Containing Extract of Isodon japonicus (Burm.) Hara which has Antioxidant Activity - Google Patents
Composition Containing Extract of Isodon japonicus (Burm.) Hara which has Antioxidant Activity Download PDFInfo
- Publication number
- KR20140109631A KR20140109631A KR1020130023817A KR20130023817A KR20140109631A KR 20140109631 A KR20140109631 A KR 20140109631A KR 1020130023817 A KR1020130023817 A KR 1020130023817A KR 20130023817 A KR20130023817 A KR 20130023817A KR 20140109631 A KR20140109631 A KR 20140109631A
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- South Korea
- Prior art keywords
- extract
- present
- fraction
- solvent
- ethyl acetate
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
The present invention provides Isodon japonicus extract showing antioxidative activity. The extracts and fractions of Ganoderma lucidum according to the present invention have a remarkable antioxidative effect and can be utilized as antioxidative medicines, cosmetics and functional food compositions.
Description
The present invention relates to a composition containing an antimicrobial extract having antioxidative activity, and more particularly, to a composition containing an antimicrobial extract having an antioxidant activity, Lt; / RTI > activity. The fractions according to the present invention exhibit a high antioxidative activity and thus can be used in the field of cosmetics, pharmaceuticals or food compositions.
Free radicals are atoms, ions, or molecules that have unpaired electrons. Free radicals, especially free radicals derived from oxygen molecules, play a fundamental role in many biological phenomena. Excessive concentrations of various types of oxygen and free radicals can have severe adverse effects such as peroxidation of membrane lipids in vivo, hydroxylation of nucleic acid bases and oxidation of radical active moieties in sulfhydryl groups and other proteins, If not suppressed, it can lead to mutations and cell death.
Many free radical reactions cause oxidative damage to biomolecules, crosslinking proteins, mutating DNA, and oxidizing lipids. Once free radicals are formed, they interact with other free radicals and non-radical oxidizing agents. In addition, if some of the products of the free radical reaction are damaged, they can produce chemical species that can potentially cause damage. Oxygen-free radicals also cause oxidative modification of the protein. In addition, oxidative damage of free radicals has been reported to play a major role in aging and degenerative diseases such as Alzheimer's disease, rheumatoid arthritis, cancer and atherosclerosis.
Accordingly, there is a growing demand for various antioxidant substances, antioxidant-related cosmetics, antioxidant-related functional foods and antioxidant-related pharmaceutical compositions, and researches related thereto have been actively conducted. Efforts are continuing.
Isodon japonicus (Burm.) Hara) is a perennial herb in the mountains and fields of each country. The growth environment is slightly dry and grows in sunny or semi-dark. Its height is 50-100 cm. Leaves face opposite, 6-15 ㎝ in length, 3.5-7 ㎝ in width, wide ovate and pointed end. The surface of the leaf is green and the back side is light green, with fine hairs and sawtooth on the edge. The flower is light purple and its length is about 0.5-0.7 ㎝. It faces at the end of main stem with leaf axil. Fruits run oval in October. It is used for edible use, and for medicinal purposes.
JP-A-2009-212634 (entitled "ANTI-AGING AGENT AND EXTERNAL PREPARATION FOR SKIN") discloses an anti-aging composition comprising vitamin C, ginger extract and / -236620 (the name of the invention: SKIN CARE PREPARATION FOR EXTERNAL USE) discloses a cosmetic composition for repairing damaged skin containing vitamin D, ginger extract, soap grass extract and the like. However, as in the present invention, there is no document which identifies the fractions exhibiting the specific antioxidant activity of the extract of Fusarium oxysporum.
Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.
The present inventors have made extensive efforts to solve the above problems. As a result, it was found that the fractions of Radix grass extract showed excellent antioxidative effect, and thus the present invention was completed.
Accordingly, it is an object of the present invention to provide an antimicrobial extract that exhibits antioxidative effects.
Another object of the present invention is to provide a cosmetic composition, a pharmaceutical composition or a cosmetic composition containing the above-described anti-inflammatory agent.
Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.
The present invention provides an extract of Nymphena vulgaris.
The present inventors have made intensive researches for screening plant materials showing excellent antioxidative effects. As a result, it was confirmed that the fraction of Radix grass extract showed excellent antioxidative effect.
According to one aspect of the present invention, the present invention provides Isodon japonicus extract showing antioxidative activity.
According to a preferred embodiment of the present invention, the gum paste may preferably be selected from the group consisting of flowers, leaves, stems, fruits, seeds, roots and outpones of the gum grass, more preferably, , Stem, fruit, roots, and outposts, and most preferably may be the outpost of an inflorescences. In the present specification, the most preferable extraction site of the ganoderma powder is the outpouring because it proves that the ganoderma superabsorbent plant is superior to the antioxidant effect through the examples described later.
As used herein, the term " extract " used in reference to an anti-gingival herb extract is intended to include not only the extract obtained by treating the anti-gingu extract with an extraction solvent, but also the formulation (e.g., powdered) It also has a meaning including a workpiece.
If the extract used in the composition of the present invention is obtained by treating the extractant with the extraction solvent, various extraction solvents may be used. (B) an anhydrous or a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, propanol, butanol, n-propanol, iso-propanol and n-butanol, (E) ethyl acetate, (f) chloroform, (g) 1,3-butylene glycol, (h) hexane, (i) diethyl ether, or (iii) a mixed solvent of a lower alcohol and water. (j) The extract can be obtained by treating butyl acetate with an anti-fungal agent.
As used herein, the term " extract " has the meaning conventionally used in the art as a crude extract, but broadly includes fractions obtained by further fractionation of the extract. That is, the extracts of Yanseonggul have not only been obtained by using the above-mentioned extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the above extract through an ultrafiltration membrane having a constant molecular weight cut-off value, and a separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) The fractions obtained by the purification method are also included in the extract of Ganoderma lucidum according to the present invention.
The gilt-grass extract used in the present invention may be prepared in powder form by an additional process such as vacuum distillation and freeze-drying or spray-drying.
According to a preferred embodiment of the present invention, the ganoderma extract is preferably selected from the group consisting of hexane, chloroform, butanol, ethyl acetate, and Aqueous ), More preferably an antioxidant activity fraction of a solvent selected from the group consisting of hexane, chloroform, butanol, and ethyl acetate, and most preferably, May be an antioxidant activity fraction of an ethyl acetate solvent.
The reason why the most preferable fraction solvent in the present invention is ethyl acetate is that the most remarkable antioxidative effect was obtained when the ganoderma extract was fractionated using ethyl acetate.
According to a preferred embodiment of the present invention, the fraction may be a butein-based compound having a structure represented by the following formula (1).
According to a preferred embodiment of the present invention, the fraction may exhibit DPPH radical scavenging ability, ABTS radical scavenging ability and reducing power by FRAP.
According to a preferred embodiment of the present invention, the amount of the ganoderma extract is preferably 0.00002 to 30 wt%, more preferably 0.0001 to 10.0 wt%, and most preferably 0.0025 to 10 wt% based on the total weight of the cosmetic, food or pharmaceutical composition. 1.0% by weight.
In the composition of the present invention, the preferred amount of the extract is from 0.00002 to 30% by weight. When the content of the extract is less than 0.00002% by weight, the antioxidative effect can not be sufficiently obtained. And there is a problem of safety due to cytotoxicity when it is exceeded.
According to a preferred embodiment of the present invention, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray ≪ / RTI >
According to a preferred embodiment of the present invention, the Radix grass extract exhibiting antioxidative activity can be used in pharmaceutical compositions and food compositions in addition to the above-mentioned cosmetic compositions. As a result of the search for KFDA food raw materials ( http://fse.foodnara.go.kr ), the banana paste has no problem in using as a pharmaceutical composition or a food composition legally, and has an excellent effect on anticancer and analgesia from ancient times. Can be used in food or pharmaceutical compositions that exhibit antioxidant effects in light of the known facts.
The cosmetic composition of the present invention may contain other ingredients in addition to the above-mentioned gum arabic extract. According to an embodiment of the present invention, the cosmetic composition of the present invention is obtained from the group comprising glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin Further comprising at least one auxiliary component selected from the group consisting of glycerin, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, squalane and sodium citrate, and at least one auxiliary ingredient selected from the group consisting of butylene glycol, citric acid, panthenol and allantoin And most preferably at least one component selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin .
Since the cosmetic composition of the present invention is basically applied to the skin, it can be provided with reference to the cosmetic composition of the related art, for example, as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, But are not limited to, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.
When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .
When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.
When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.
When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.
When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.
According to a preferred embodiment of the present invention, the composition of the present invention may be a pharmaceutical composition having an excellent antioxidative effect.
When the composition of the present invention is manufactured from a pharmaceutical composition, the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier. The pharmaceutically acceptable carriers to be contained in the pharmaceutical composition of the present invention are those conventionally used in the formulation and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, But are not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrups, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. It is not. The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc. in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).
The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans in various routes such as oral or parenteral routes such as oral, rectal or intravenous, muscular, subcutaneous, intra-uterine, Can be administered by injection. Preferably, it is applied by transdermal administration during parenteral administration, more preferably by topical application by application.
The appropriate dosage of the pharmaceutical composition of the present invention may vary depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate, . The dose of the pharmaceutical composition of the present invention can be administered in an amount of 0.1-100 mg / kg on an adult basis once or several times a day in the case of an oral formulation, and in the case of an external preparation, It is preferable to apply it once to 5 times a day in an amount of 3.0 ml and continue for 1 month or longer. However, the dosage is not intended to limit the scope of the present invention.
The pharmaceutical composition of the present invention may be formulated into a unit dose form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art to which the present invention belongs. Or by intrusion into a multi-dose container. The formulations may be in any form suitable for pharmaceutical preparations including oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, external preparations such as ointments and creams, suppositories and sterile injectable solutions, , Dispersants, or stabilizers.
According to a preferred embodiment of the present invention, the composition of the present invention may be a food composition having an excellent antioxidative effect.
When the composition of the present invention is prepared with a food composition, it includes not only the active ingredient, but also ingredients normally added during the manufacture of the food, including, for example, proteins, carbohydrates, fats, nutrients, flavoring agents and flavoring agents . Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings.
For example, when the food composition of the present invention is prepared as a drink, it may further contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, mulberry extract, jujube extract, licorice extract, have.
As a result of analyzing cell viability with respect to the active ingredient of the composition of the present invention in the concrete examples of the present invention, it was found that the active ingredient of the composition of the present invention is a harmless substance as a natural substance. Therefore, since the active ingredient of the present invention has little toxicity and side effects, it can be safely used even for long-term use, and can be safely applied to cosmetic, pharmaceutical and food compositions as described above.
The features and advantages of the present invention are summarized as follows:
(a) The present invention provides Isodon japonicus extract showing antioxidative activity.
(b) The extracts and fractions of Gramineae according to the present invention can be utilized as antioxidative medicines, cosmetics and functional food compositions, so that the application range of the invention is very wide.
(c) Since the herbaceous plant of the present invention is a plant which does not have a difficult growth condition and lives well in Korea's climate, it is possible to obtain a large amount of raw materials for antioxidant-related product composition easily and inexpensively. This results in lowering the price of the product using the extract and the fraction.
(d) Since the process for obtaining the extract and fractions of Glycyrrhiza glabrugula according to the present invention is not complicated, it is very easy to apply to the field of industry, and accordingly, the price of the product using the extract and the fraction can be inexpensively adjusted.
(e) In the case of the extracts and the fractions of the scutellariae of the present invention, the antioxidant activity is excellent because it contains a large amount of butane-based compounds.
(f) Antioxidant-related medicines, cosmetics and functional food compositions containing the extracts and fractions of the present invention as an active ingredient do not cause any adverse effects on the human body.
Fig. 1 shows a specific method for preparing an extract of Ganoderma lucidum according to the present invention.
Fig. 2 shows the results of NMR measurement of the purified compound of the extract of Fungus pullulatus of the present invention.
Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.
Example
Throughout this specification, "%" used to denote the concentration of a particular substance is intended to include solids / solids (wt / wt), solid / liquid (wt / The liquid / liquid is (vol / vol)%.
Manufacturing example
Manufacturing example 1: Preparation of 70% ethanol extract
(Shaker SK-71 JEIO TECH, Daejeon, Korea) was added to 100 g of Isodon japonicus powder, which had been pulverized with 40 mesh, by 10
Manufacturing example 2: Preparation of solvent fraction extract using 70% ethanol extract
The sequential solvent fraction was prepared by dissolving 10 g of the above 70% ethanol extract in 500 mL of water, adding 500 mL of hexane, which is a low polarity solvent, into the separating funnel, and extracting hexane fraction three times. The water layer was added to the separating funnel, and the fractional extract was obtained by using the polarity difference in the order of chloroform, ethyl acetate, butanol and aqueous in the same manner as described above (see FIG. Each of the separated solvent fraction extracts was concentrated under reduced pressure to remove the solvent, and then lyophilized at -70 ° C to obtain the yield of each fraction. A predetermined amount of the fraction was used as a sample for analyzing antioxidant activity.
Experimental Method
Experimental Example 1: total polyphenol content
To the 20 μL of each solvent-extracted fraction dissolved at a concentration of 1 mg / mL, 50 μL of Folin-Ciocalteau reagent and 200 μL of 7.5% sodium carbonate were added in that order. After standing at room temperature for 60 minutes, absorbance was measured at 765 nm. The total polyphenol content was calculated from the calibration curve prepared by analyzing gallic acid in the same manner as that of the reference material.
Experimental Example 2: total flavonoid content
20 μl of the fraction extract of each solvent dissolved at a concentration of 1 mg / mL was mixed with 75 μL of sodium nitrite solution and 100 μL of the third distilled water, reacted for 6 minutes, added with 20 μL of aluminum chloride solution, reacted for 5 minutes, And the absorbance at 510 nm was measured. The content of total flavonoid was determined from the standard curves prepared with quercetin.
Experimental Example 3: DPPH Radical Scatters
40 μL of each fraction extract and 160 μL of 1,1-diphenyl-2-picryhydrazyl (DPPH) solution were mixed well and reacted in a dark place for 30 minutes. Then, the absorbance at 518 nm was measured to confirm DPPH radical scavenging ability. Free radical scavenging activity as a percentage compared to the sample without addition.
Experimental Example 4 : ABTS Radical Scatters
The radical scavenging activity of ABTS [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate)] was determined by the removal of the ABTS free radicals produced by the reaction with Potassium persulfate by the antioxidant of the sample, The results are shown in Fig. 7.4 mM ABTS solution and 2.6 mM potassium persulfate were mixed and reacted in a dark room for about 12 hours. This was diluted to 731 nm with an absorbance of 1 ± 0.1. Then, 285 μL of the extract was mixed with 15 μL of the fractional extract of each solvent. The mixture was reacted in a dark place for 30 minutes, and the absorbance at 734 nm was measured. The absorbance difference between the sample and the non- Respectively.
Experimental Example 5: Ferric 감 antioxidant power ( FRAP )
The FRAP reagent was diluted 10: 1: 1 (v / v / v) with 10 mM TPTZ (2,4,6-tripyridyl-s-triazine) and 20 mM FeCl3H2O in 300 mM acetate buffer ), And the mixture was heated at 37 ° C for 5 minutes and used as a FRAP reagent. 150 μL of the prepared reagent was mixed with 50 μL of each concentration and reacted at 37 ° C for 4 minutes. The absorbance was measured at 593 nm and calculated from the standard curve obtained using FeSO 4 7H 2 O as a standard.
Experimental Example 6: Fe 2 + chelating activation
Fe 2 + chelating activity of the sample 50 μL, 0.6 mM FeCl24H2OIron (Ⅱ ) chloride tetrahydrate] solution 10 μL of distilled water and 90 μL were mixed and reacted after 10 minutes at room temperature. After the reaction, 20 μL of 5 mM ferrozine [3- (2-pyridyl) -5,6-diphenyl-1,2,4-triazine-4 ', 4 "-disulfonic acid) solution was mixed and reacted at room temperature for 5 minutes Absorbance was measured at 562 nm.
Experimental Example 7: Separation, purification and structural analysis of antioxidant active substances
One purified compound (BA1) was obtained by semi-prep LC autopurification (Waters 2525 Binary Gradient Module, Milford, MA, USA) system for the isolation and purification of ethyl acetate fraction extract. The analytical conditions were as follows: water (solvent A) containing 0.1% formic acid and methanol (solvent B) containing 0.1% formic acid were used for the mobile phase, and synerg Polar-RP 80A (Penomenex, USA, * 10.0 mm) column was used. The flow rate was 3.8 mL / min, the injection volume was 50 μL, and the detector was set at 190-500 nm. ≪ 1 > H-NMR (400MHz, CD3OD).
Experiment result
Experimental Results 1: Extraction yield
The extraction yields of the solvent fractions of A. glabrata were highest in the aqueous fraction fractions having a high polarity as shown in Table 1 below, and the lowest in the hexane fraction fractions having a low polarity.
Experimental Result 2: Phenol and flavonoid content
Total phenol and total flavonoid contents As shown in Table 2, the majority of the flavonoid glycosides were transferred to the ethyl acetate fraction extract, and the total phenol and total flavonoid contents were higher in the ethyl acetate fraction extract than in the other fraction extracts.
1) Total phenolic content was expressed as mg / g gallic acid equivalent (GAE)
2) Total phenolic content was expressed as mg / g quercetin equivalent (QUE)
Experimental Result 3: Gum pool Extract DPPH Radical Scatters
The DPPH radical scavenging activity of the FROG extract was not higher than that of the natural antioxidants as shown in Table 3, but showed a concentration-dependent activity. Especially, the ethyl acetate fraction extract at 500 μg / mL showed higher DPPH radical scavenging ability than BHT, a synthetic antioxidant.
Experimental Result 4: Gum pool Extract ABTS Radical Scatters Measure
The ABTS radical scavenging activity of the FROG extract was not higher than that of the natural antioxidants as shown in Table 4, but showed a concentration-dependent activity. Especially, ethyl acetate fraction extract at 62.5 μg / mL showed higher ABTS radical scavenging ability than α-tocopherol, a natural antioxidant.
Experimental Results 5: FRAP Measurement of reducing power by
The reducing power of ferric reducing antioxidant power (FRAP) was not higher than that of natural antioxidants as shown in Table 5, but showed a concentration-dependent activity. Especially, the ethyl acetate fraction of 250 μg / mL concentration showed higher reducing power than BHT, a synthetic antioxidant, and α-tocopherol, a natural antioxidant, by FRAP.
Results 6: Fe 2 + chelating Active measurement
The Fe 2 + chelating activity showed a concentration-dependent activity as shown in Table 6 below. Chloroform fraction extract showed the highest activity at the concentration of 1,000 μg / mL, 94.3% and 17.1% in the ethyl acetate fraction extract.
Experimental Result 7: Gum pool Of the purified compound of the fraction extract DPPH Radical Scatters Measure
The DPPH radical scavenging activity of the purified compound (BA1) isolated from the ethyl acetate fraction extract of Angelica keiskei koidz. Showed higher activity than the natural antioxidant and the synthetic antioxidant as shown in Table 7. In particular, BA1 at 12.5 μg / mL showed higher DPPH radical scavenging ability than ascorbic acid, α-tocopherol and BHT, which are synthetic antioxidants.
Experimental Results 8: Gum pool Of the purified compound of the fraction extract ABTS Radical Scatters Measure
The ABTS radical scavenging activity of the purified compound (BA1) isolated from the ethyl acetate fraction extract of Panax annuus was higher than that of natural antioxidants and synthetic antioxidants as shown in Table 8 below. In particular, BA1 at 6.3 μg / mL showed higher ABTS radical scavenging activity than ascorbic acid, α-tocopherol and BHT, which are synthetic antioxidants.
Experimental Results 9: Gum pool Of the purified compound of the fraction extract FRAP Reduction power measurement
As shown in Table 9, the reducing power of the purified compound (BA1) isolated from the extract of ethyl acetate fraction was higher than that of natural antioxidants and synthetic antioxidants. In particular, BA1 at 3.1 μg / mL showed higher reducing power than ascorbic acid, α-tocopherol, and BHT, a synthetic antioxidant.
Results 10: Gum pool Of the purified compound of the fraction extract NMR Measurement result
Analysis of the purified compound (BA1) isolated from the ethyl acetate fraction of Angelica keiskei koidaceans by 1 H-NMR (400 MHz,
Example : Gum pool Manufacture of cosmetics
Example 1: Manufacture of softening longevity
Examples of the formulations of the flexible lotion in the cosmetic composition containing the extract of the present invention of the present invention are as follows.
Example 2: Manufacture of Lotion
Examples of the formulations of milk lotion in cosmetics containing the extract of the present invention of the present invention are as follows.
Example 3: Manufacture of nutritional cream
Examples of the formulations of the nutritional cream among the cosmetics containing the extract of the present invention include the following.
Example 4 : Massage Manufacture of cream
Examples of the formulations of the massage cream of the cosmetic composition containing the extract of the present invention of the present invention are as follows.
Example 5: Manufacture of pack
Examples of formulations of packs of cosmetics containing the extracts of the present invention include the following.
Claims (13)
Wherein the flock is selected from the group consisting of leaves, stems, fruits, roots, and toppings of the flies.
Wherein the extracting solvent of the extract is selected from the group consisting of water, ethanol, methanol, methylene chloride, ethyl acetate, butanol, nucleic acid, ethanol, butylene glycol, chloroform and mixtures thereof.
Wherein the extracting solvent of the extract is ethanol.
The ganoderma lucidum extract may be prepared by adding an antioxidant activity of a solvent selected from the group consisting of hexane, chloroform, butanol, ethyl acetate and Aqueous Wherein the extract is a fraction.
Wherein the ganoderma extract is an antioxidant activity fraction of an ethyl acetate solvent of a ganoderma complex ethanol solvent extract.
Wherein said fraction contains as an active ingredient a butein-based compound having a structure represented by the following formula (1).
[Chemical Formula 1]
Wherein said fraction exhibits DPPH radical scavenging ability, ABTS radical scavenging ability and reducing power by FRAP.
The cosmetic composition according to any one of claims 1 to 3, wherein the amount of the extract is from 0.00002 to 30% by weight based on the total weight of the cosmetic composition.
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Or a pharmaceutically acceptable salt thereof.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020130023817A KR20140109631A (en) | 2013-03-06 | 2013-03-06 | Composition Containing Extract of Isodon japonicus (Burm.) Hara which has Antioxidant Activity |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020130023817A KR20140109631A (en) | 2013-03-06 | 2013-03-06 | Composition Containing Extract of Isodon japonicus (Burm.) Hara which has Antioxidant Activity |
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| Publication Number | Publication Date |
|---|---|
| KR20140109631A true KR20140109631A (en) | 2014-09-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020130023817A KR20140109631A (en) | 2013-03-06 | 2013-03-06 | Composition Containing Extract of Isodon japonicus (Burm.) Hara which has Antioxidant Activity |
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| Country | Link |
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| KR (1) | KR20140109631A (en) |
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