KR20140045870A - Germination or growth improvement method of vegetation using biopolymer - Google Patents

Abstract

The present invention relates to a germination or growth improvement method of vegetation, and specifically, to an environmentally friendly germination or growth improvement technique of vegetation including a step of adding a high molecule viscous biopolymer, which is polysaccharide generated from a plant body, to soil. The high molecule viscous biopolymer includes a polysaccharide based or amino acid based biopolymer. The polysaccharide based high molecule viscous biopolymer includes a high molecule chain type biopolymer or a gelated biopolymer. [Reference numerals] (AA) Artificial compost(beta-glucan, xanthan gum, and non-treated sample from the left); (BB,FF) Seven days; (CC,GG) 10 days; (DD,HH) 14 days; (EE) Red clay(beta-glucan, xanthan gum, and non-treated sample from the left)

Description

How to improve germination or growth of vegetation using biopolymer {GERMINATION OR GROWTH IMPROVEMENT METHOD OF VEGETATION USING BIOPOLYMER}

The present application relates to a method for promoting germination or growth of growth, and a composition of soil for germination or growth enhancement of vegetation. Specifically, the present invention relates to an environmentally friendly vegetation germination or growth enhancement technique using a biopolymer, which is a polysaccharide produced from an organism.

The geotechnical structure of the soil directly affects vegetation growth. In general, the looser the soil structure and the higher the water content in the soil, the better the plant growth [Passioura, 1991, "Soil structure and plant growth", Australian Journal of Soil Research, Vol. 28, No. 6, 717 -728 pages]. Therefore, in agriculture, it is important to stir the farmland before sowing or to maintain an efficient irrigation system. Most of the topsoil in Korea is granite residue, which is the final weathering product of granite. Hwang Jin Yeon et al., 2000, "Organic Minerals and Chemical Components of Korean Ocher (Weathered Soil), Korean Mineral Society, Vol. 13, No. 3, pp. 146-163] Ocher is composed mainly of halloysite and its compact structure has been used as a building material since ancient times, but it has been recognized as not suitable for vegetation growth.

Thus, while preventing the erosion of the soil, interest in research to improve the soil to increase the vegetation growth is increasing.

On the other hand, the biopolymer is a high-molecular substance produced by living organisms refers to various kinds of high-molecular substances in which carbohydrates, fats, proteins, nucleic acids, and complexes thereof, are synthesized in the organism and secreted out of the body. Among the industrially important biopolymers are xanthan and gellan produced by microorganisms, and chitosan obtained by deacetylation of chitin [Lee Jin-woo, 2001, "Gum using microorganisms. Production, "Microbiology and Industry, Vol. 27, pp. 23-32. In particular, water-soluble or insoluble polysaccharides are representative biopolymers that are useful in humans, with at least 10 monosaccharides or derived monosaccharides being the most abundant in nature as polymers produced by glycosidic linkages. Unlike chemical synthetic polymers, they are environmentally friendly, and due to their functional characteristics such as gel formation, emulsion stability, surface tension control, water absorption, adhesion, lubrication, and biofilm formation, which are derived from structural properties, It is used as a major material in the field [Hong Geum Lee, 2001, "Trend of Biopolymer Development from Marine Microorganisms", BioWave, Vol. 3, No. 4, SubNo. 7].

Such biopolymers, unlike petroleum-based synthetic polymers, are environmentally friendly materials, and are free from environmental and social problems, and thus have the potential to be used as important material materials in the future.

Accordingly, the present application is to provide a method for promoting germination or growth of vegetation, and a soil composition for promoting germination or growth of vegetation prepared by the method, which can enhance vegetation growth using a biopolymer.

However, the problems to be solved by the present invention are not limited to the problems described above, and other problems not described can be clearly understood by those skilled in the art from the following description.

A first aspect of the present application provides a method for promoting germination or growth of vegetation, comprising adding a polymer viscous biopolymer to soil.

The second aspect of the present application provides a soil composition for promoting germination or growth of vegetation, which is prepared by the method of promoting germination or growth of vegetation of the first aspect of the present application, and includes a polymer viscous biopolymer.

According to the present application, it is possible to prepare a soil composition for promoting germination or growth of environmentally friendly vegetation that does not depend on the existing nitrogen, phosphorus-based fertilizer or artificial culture soil. Conventional vegetation promotion techniques are mainly using artificial chemical fertilizers such as nitrogen and phosphorus, or artificial culture soil. However, the method for promoting germination or growth of vegetation using the biopolymer according to the present application not only promotes germination and / or growth of vegetation, but also maintains the initial stabilization of the ground before vegetation stabilization, and contaminates and eutrophicates to groundwater or rivers. Not only is it a concern, it has the environmental advantages that biopolymers naturally biodegrade and return to the original soil over time. Therefore, the method of promoting germination or growth of vegetation using the biopolymer according to the present application is not only in the field of ecological vegetation composition, but also in the construction of vegetation surface at large construction sites, river banks and waterfront space greening projects, initial stabilization of road and railway slopes, and large-scale farmland. It can be effectively used in various fields such as composition, rooftop and urban agriculture. Furthermore, the germination or growth promotion method of the vegetation using the biopolymer according to the present application and the soil composition for germination or growth enhancement of the vegetation can be expected to further effect the invention in accordance with the trend of full-scale biopolymer commercialization.

1 is a conceptual diagram illustrating a biopolymer-treated vegetation ground composition using a spraying method according to an embodiment of the present application.
2 is a conceptual diagram illustrating a biopolymer-treated vegetation ground composition using a wet mixing and spreading method according to an embodiment of the present application.
3 is a conceptual diagram illustrating a biopolymer-treated vegetation ground composition using a dry mixing / spray method according to an embodiment of the present application.
Figure 4 shows a conceptual diagram of the partition division for eco-friendly waterfront space composition using a biopolymer according to an embodiment of the present application.
Figure 5 shows a test cultivation picture of the biopolymer treated soil according to an embodiment of the present application.
Figure 6 shows the vegetation growth results with time of the biopolymer treated soil according to an embodiment of the present application.
Figure 7a is an electron projection micrograph showing the untreated loess and vegetation roots according to an embodiment of the present application.
Figure 7b is an electron projection micrograph showing the beta glucan treated loess and vegetation roots according to an embodiment of the present application.
Figure 7c is an electron projection microscope photographed the xanthan gum treated loess and vegetation roots according to an embodiment of the present application.

Hereinafter, embodiments and examples of the present invention will be described in detail with reference to the accompanying drawings, so that those skilled in the art can easily carry out the present invention.

It should be understood, however, that the present invention may be embodied in many different forms and is not limited to the embodiments and examples described herein. In the drawings, the same reference numbers are used throughout the specification to refer to the same or like parts.

Throughout this specification, when a part is referred to as being "connected" to another part, it is not limited to a case where it is "directly connected" but also includes the case where it is "electrically connected" do.

Throughout this specification, when a member is "on " another member, it includes not only when the member is in contact with the other member, but also when there is another member between the two members.

Throughout this specification, when an element is referred to as "comprising ", it means that it can include other elements as well, without departing from the other elements unless specifically stated otherwise.

As used herein, the terms "about," " substantially, "and the like are used herein to refer to or approximate the numerical value of manufacturing and material tolerances inherent in the stated sense, Accurate or absolute numbers are used to prevent unauthorized exploitation by unauthorized intruders of the mentioned disclosure. Also, throughout the present specification, the phrase " step "or" step "does not mean" step for.

Throughout this specification, the term "combination (s) thereof " included in the expression of the machine form means a mixture or combination of one or more elements selected from the group consisting of the constituents described in the expression of the form of a marker, Quot; means at least one selected from the group consisting of the above-mentioned elements.

Throughout this specification, the description of "A and / or B" means "A or B, or A and B".

Throughout this specification, the term "cationic aqueous solution" refers to an aqueous solution containing a cation, and may include, for example, an aqueous solution containing an alkali metal or alkaline earth metal ion, but may not be limited thereto. The alkali metal comprises a Group 1 metal consisting of Li, Na, K, Rb, and Cs, which can provide monovalent cations, and the alkaline earth metals can provide divalent cations, Be, Mg, Ca , Group 2 metals consisting of Sr, Ba, and Ra.

Throughout this specification, the term "Hwang-to" means yellowish or yellowish brown granite, in which fine grains of rock broken by weathering in the interior of the continent are blown off and stacked.

Throughout this specification, the term "soil" is used in the same sense as the soil.

Hereinafter, embodiments of the present invention are described in detail, but the present invention is not limited thereto.

A first aspect of the present application provides a method for promoting germination or growth of vegetation, comprising adding a polymer viscous biopolymer to soil.

Germination and growth of vegetation are affected by the aeration and moisture permeability of the soil, the moisture retained, temperature, light quantity, and organic matter content. Therefore, in order to improve the germination and growth of vegetation in general, methods for improving the organic content in soil by using artificial culture soil having excellent ventilation, moisture permeability, and soil moisture retention rate, or by adding nitrogen and phosphorus chemical fertilizers are commonly used. . However, in the case of large-scale planting or vegetation composition, the use of artificial culture soil is extremely unrealistic, and even nitrogen and phosphorus-based chemical fertilizers can cause water pollution when introduced into rivers and groundwater. I have it.

In one embodiment of the present disclosure, the polymer viscous biopolymer may be used without limitation as long as it is a polymer material produced from an organism, but may not be limited thereto. The polymer viscous biopolymer may include a substance having glucose as a basic unit, and may be broadly classified into a polysaccharide and an amino-acid series. Biopolymers can be classified into high-molecular chains and gelation biopolymers according to their shape. For example, the polymer chain biopolymer may include a substance having glucose as a basic unit, for example, beta-1,3 / 1,6-glucan (Polycan ^™ ). , Alpha glucan, Curdlan, and the like, and the gelling biopolymer may include Welan gum, Gellan gum, Xanthan gum, Agar gum, Succino Glycan (Succinoglycan gum) and the like, but may not be limited thereto. The amino acid-based biopolymer may include chitosan, gamma fiji, and the like, but may not be limited thereto.

In one embodiment of the present application, the polymeric viscous biopolymer is about 20 parts by weight or less, for example, about 0.00001 parts by weight to about 15 parts by weight, about 0.00001 parts by weight to about 10 parts by weight based on about 100 parts by weight of soil About 0.00001 parts by weight to about 5 parts by weight, about 0.00001 parts by weight to about 1 parts by weight, about 0.00001 parts by weight to about 0.5 parts by weight, about 0.00001 parts by weight to about 0.1 parts by weight, about 0.0001 parts by weight to about 20 parts by weight About 0.01 parts by weight to about 20 parts by weight, about 0.05 parts by weight to about 20 parts by weight, about 0.1 parts by weight to about 20 parts by weight, about 0.5 parts by weight to about 20 parts by weight, about 1 parts by weight to about 20 parts by weight Part, about 5 parts by weight to about 20 parts by weight, or about 10 parts by weight to about 20 parts by weight, but may not be limited thereto.

In one embodiment of the present application, the polymer viscous biopolymer may be to expand the pores in the soil, to maintain the soil water properties, and to increase the binding force between the soil particles, but may not be limited thereto.

In one embodiment of the present application, adding the polymer viscous biopolymer to the soil is performed by mixing the polymer viscous biopolymer with the soil, spraying on the surface of the soil, or injecting into the soil It may be, but may not be limited thereto.

In one embodiment of the present application, but may include adding the polymer viscous biopolymer to the soil in a powder state, but may not be limited thereto. The polymer viscous biopolymer may be directly mixed with soil, or may be coated with a polymer viscous biopolymer powder or a suspension or an aqueous solution on the surface of the soil to form a coating, or injected into the soil, but is not limited thereto. It may not be. In addition, after directly mixing the polymer viscous biopolymer with the soil, it may be installed on the surface of the target area, but may not be limited thereto.

In one embodiment of the present application, it may include adding the polymer viscous biopolymer to the soil in an aqueous solution or a basic aqueous solution, but may not be limited thereto. For example, a suspension or an aqueous solution of the polymer viscous gelling polysaccharide biopolymer may be added as it is, or a salt may be added to the suspension or the aqueous solution of the biopolymer to prepare a basic aqueous solution, for example, a basic aqueous solution having a pH of about 9 or more. It may be added to the soil by lowering the viscosity, but may not be limited thereto. After the basic aqueous solution of the biopolymer is added to the soil, the acidic aqueous solution may be sprayed to promote aggregation of the infiltrating polymer viscous gelled polysaccharide biopolymer, but may not be limited thereto.

In one embodiment of the present application, after adding the polymer viscous biopolymer to the soil, it may further include adding a cation of an alkali metal or alkaline earth metal, but may not be limited thereto. For example, a cation of an alkali metal such as Na ⁺ , K ⁺ or an alkali earth metal such as Ca ^{2 +} , Mg ^{2 +} may be added to induce gelation of the biopolymer to form a solid soil-biopolymer mixture. However, this may not be limited.

In one embodiment of the present application, after adding the polymer viscous biopolymer to the soil, it may further include adding an acidic aqueous solution or a cationic aqueous solution of pH about 5 or less, but is not limited thereto. Can be. The cationic aqueous solution may include, for example, an aqueous solution containing alkali metal or alkaline earth metal ions.

Since the surface of the biopolymer used in the germination or vegetation enhancement method of the vegetation using the biopolymer according to the present application has a negative charge, when the alkali metal or alkaline earth metal ions are added to the soil, the bonding properties with the soil are further increased. Can be improved.

In one embodiment of the present application, after adding the polymer viscous biopolymer to the soil, it may further include heating and cooling, but may not be limited thereto. For example, the polymer viscous biopolymer may be added to the soil, and then sufficiently heated at about 80 ° C. to about 120 ° C., and then cooled to about 40 ° C. to about 60 ° C. or less to induce gelation of the biopolymer. This may not be limited. Further, after the cooling, the method may further include adding a cation of an alkali metal or an alkaline earth metal, for example, an alkali metal cation such as Na ⁺ , K ⁺ , or an alkali earth metal such as Ca ^{2 +} , Mg ^{2 +,} or the like. It may be, but may not be limited thereto.

In one embodiment of the present application, after spraying the polymer viscous biopolymer to the surface of the soil, may further include spraying water and / or acidic aqueous solution, but may not be limited thereto. For example, an acidic aqueous solution having a pH of about 5 or less may be sprayed to strengthen the gel structure of the biopolymer in the soil, but may not be limited thereto.

In one embodiment of the present application, the polymer viscous biopolymer may be added to the soil in various ways as follows according to the type and purpose of the polymer viscous biopolymer used, but may not be limited thereto:

1.Method for Enhancing Soil Vegetation of Polymer Viscous Chain Polysaccharides

In order to improve the vegetation of soil, a high molecular viscous chain polysaccharide biopolymer is used. Polymeric viscous chain-type polysaccharides have high hydrophilicity to maintain the moisture environment in the soil, as well as improve the aeration and water permeability of the soil, and further improve the growth of plant roots, thereby promoting overall vegetation. Specific implementation method is as follows.

1) Composition of Vegetation Soil Using Biopolymer Mixed Soil

Promoting the germination and growth of the plant by directly cultivating the plant using a mixed soil containing a polymer viscous chain polysaccharide biopolymer of about 0.0001% to about 5% relative to the dry weight of the soil as vegetation soil.

2) Vegetation cultivation using biopolymer suspension or aqueous solution

In the vegetation cultivation method, the use of a polymer viscous chain polysaccharide suspension or an aqueous solution of about 0.00001% to about 10% as a growing water is used to suppress the loss of water supplied and to improve the durability of the soil around plants. At the same time it is effective in preventing plant growth.

2. Method for Improving Penetration of Dirt Viscous Biopolymer in Soil

Polymeric viscous chain polysaccharide biopolymers are generally polymers having a molecular weight of about 10,000 Da or more, and the fibers are entangled with each other in a neutral or acid (pH up to about 7) suspension or in an aqueous solution to show high viscosity. In particular, the viscous chain-type polysaccharides having a negative charge on the surface has a characteristic of increasing viscosity as the pH is lowered. Polymeric viscous chain polysaccharide biopolymers, on the other hand, swell due to high hydrophilicity and become very viscous suspensions or aqueous solutions. Thus, in order to increase the permeability of the polymer viscous biopolymer in the soil, the viscosity must be lowered. To this end, the present application proposes the following methods.

1) Method using chemical treatment

Increasing the pH of the polymer viscous chain or gelled polysaccharide suspension or aqueous solution at a concentration of about 0.00001% to about 10% results in a lower viscosity. Injecting low-viscosity biopolymer suspensions or aqueous solutions into the ground by spraying or pressure can improve penetration or diffusion into the ground.

After sprinkling or injecting a basic polymer viscous chain polysaccharide suspension or aqueous solution into the soil, additionally spraying or injecting a low pH acidic aqueous solution (pH of about 5 or less) to the viscous chain biopolymer in the soil-biopolymer mixed soil. Aggregation and viscous gelling may be promoted between the biopolymers.

2) using physical processing

A bead mill or the like can be used to lower the viscosity of the polymer viscous chain polysaccharide biopolymer solution, and the tangled polysaccharide chains can be released by stirring the solution using the beads at a rate of about 10,000 ppm or more.

In addition, the polymer viscous chain polysaccharide biopolymer solution may be collided at high pressure (about 150 bar or more) to release physically entangled polysaccharide chains. Polycan ^TM , a chain polysaccharide biopolymer solution, has a viscosity of about 1,000 cps, and when it is collided with a homogenizer at 200 bar, the viscosity decreases to about 30 cps, and the liquid of about 30 cps is again returned. Collision reduces the viscosity to about 16 cps.

Viscous chain biopolymer in soil-biopolymer mixed soils by mixing or injecting a physically viscous polymer viscous chain polysaccharide biopolymer with soil, followed by additional spraying or injection of an acidic pH solution (pH less than about 5) Can promote liver aggregation.

3) How to use heat treatment

Sufficient heating of the polymer viscous gelled polysaccharide suspension or aqueous solution at a concentration of about 0.00001% to about 10% to about 80 ° C. to about 120 ° C. lowers the viscosity of the biopolymer suspension or aqueous solution. When the mixture is mixed or injected into the soil at a high temperature, it cools naturally and forms a gel at a temperature of about 40 ° C. to about 60 ° C. or less to form a solid soil-biopolymer mixed soil.

In one embodiment of the present application, the biopolymer may be added to the soil in various ways for various purposes in various target areas as follows, but may not be limited thereto:

1. Vegetation ground composition method using polymer viscous polysaccharide biopolymer

Biopolymer treatment enhances the germination and growth of vegetation, so it can be applied to the field in various forms.

1-1. Method using spray method

The method of spraying the biopolymer suspension at high pressure can be easily applied not only to flat lands but also to inclined slopes or slopes, and dilutes the solid or liquid biopolymer at a predetermined ratio, and optionally mixes additives homogeneously. It consists of a mixing tank, a high pressure pump suitable for the high viscosity characteristics of the biopolymer suspension, a delivery tube system, and a special nozzle for effectively spraying the biopolymer mixture (see FIG. 1). 1 shows a conceptual diagram of the biopolymer treated vegetation ground composition using the spraying method as described above. The special nozzle must satisfy the conditions for spraying fine particles such as vegetation seeds.

1-2. Method using wet mixing and spreading method

It is a method of forming a biopolymer mixture soil through pre-mixing, and then laying it in a target area and forming a coating having a specific thickness through compaction. Figure 2 shows a conceptual diagram of the biopolymer treated vegetation ground composition using the wet mixed laying method associated with this. This method has the advantage of forming a homogeneous coating on the site, and through the compaction to increase the adhesion to the base.

The method consists of a device for diluting a solid or liquid biopolymer in a proportion and mixing with soil and other additives at the same time, and a compaction device for spreading and laying the installed soil (see FIG. 2). The compaction apparatus can be either roller or vibratory.

This method can improve the coating power of a coating surface in parallel with method 1-1. This method is useful when a large amount of on-site soil is available on site.

1-3. Dry mix and spray method

This method is sprayed by dry mixing and spraying method using dual transfer system without premixing, and mixed with liquid biopolymer, soil and other additives, and then attached to the ground. Use to maximize the effect. FIG. 3 shows a conceptual diagram of a biopolymer-treated vegetation ground composition using a dry mixing and spraying method.

The core of the method is the dual transport of the spreading material, the wet transport system transports and spreads the liquid biopolymer suspension, and the dry transport system transports and sprays dry soil and other additives, thereby clogging in the transfer pipe. Its purpose is to reduce construction problems and to maximize the efficiency of field work.

The system of the method includes a mixing tank for largely forming a biopolymer suspension in a liquid state, a high pressure pump and conveying tube system suitable for the high viscosity characteristics of the biopolymer suspension, a mixing tank for uniformly mixing solid soil and other additives, and a high pressure. It consists of a dual-nozzle that can be sprayed independently with a pump for the solids and the transfer tube system, liquid biopolymer and solid soil and other additives that can be transferred to the furnace (see FIG. 3).

2. Environment-friendly Landscaping Using Polymer Viscous Polysaccharide Biopolymer

The present application proposes an environment-friendly landscape composition method using a biopolymer that does not depend on existing chemical fertilizers.

In the case of rapeseed, after forming a polymer viscous polysaccharide biopolymer coating layer on the surface layer, the seeds are directly sprayed or a vegetation mat is installed. After seeding, the seed is left without post treatment or a toffee of a certain thickness is formed to protect the seed from the external environment and to induce the germination of the seed.

In the case of slopes such as a slope or a slope, a biopolymer coating layer is formed on the surface, and then the seeds are directly sprayed or a vegetation mat is applied. After sowing the seeds, they can be left without post-treatment or additional coatings of a certain thickness can be used to protect the seeds from the external environment and to promote germination.

3. Environment-friendly Waterfront Space Using Polymer Viscous Polysaccharide Biopolymer

Biopolymers are environmentally friendly and biodegradable over time, resulting in extremely low water and water ecosystem disturbances when applied to the waterfront compared to conventional cement or chemical materials. Active utilization in the furtherance is expected.

The general shape of the river and waterside space is shown in FIG. Figure 4 shows a conceptual diagram for the division of the partition for the environment-friendly waterfront space composition using the related biopolymer. It is usually divided into a bank (B), a ciliary site (C) inside the bank, and a periphery (A) outside the bank to prevent flooding. As a method for creating an environment-friendly waterfront space, the present invention performs the following implementation method for each space.

A (ambient space): All methods of method 1 can be applied depending on site conditions.

In order to suppress retrograde erosion due to river dredging and water level change, the germination or vegetation enhancement method using the biopolymer according to the present application may be applied as an environmentally friendly soil reinforcement method using the biopolymer on the surrounding ground.

B (bank): Applicable to method 1-1 or 1-3

As an alternative to the concrete blocks or sandstones used for the embankment and revetment, the germination or vegetation enhancement method of the vegetation using the biopolymer according to the present application may be applied as the method of forming the embankment and the revetment wall using the mixed biopolymer. .

In addition, the germination or vegetation enhancement method of the vegetation according to the present application can be applied as a bank surface coating method using a biopolymer in order to suppress the water penetration into the dike at the full water level or flood level.

C (Fixed ground): All methods of method 1 can be applied depending on site conditions

In order to suppress irregular soil erosion, such as partial erosion of the inflow portion or gully on the flat (coriander) due to river inflow, the germination or vegetation enhancement method according to the present application is a method for improving the soil erosion resistance using a biopolymer. Can be applied.

According to the vegetation enhancement method using the biopolymer of the present application, it is possible to prepare an environmentally friendly vegetation enhancement soil composition that does not depend on the existing nitrogen, phosphorus-based fertilizer or artificial culture. In addition, the vegetation enhancement method according to the present application by using an eco-friendly and beneficial to the human body biopolymers, it is possible to realize not only the enhancement of vegetation, but also the physical stabilization of the sowing soil until the vegetation stabilization (sufficient rooting of the root) is achieved. In particular, in the present application, the soil is treated using biopolymers, and the soil-biopolymer interaction improves the structure and moisture condition of the soil to induce the growth of plants.

The second aspect of the present application provides a soil composition for vegetation enhancement, which is prepared by the vegetation enhancement method of the first aspect of the present application, and includes a polymer viscous biopolymer.

In one embodiment of the present application, about 100 parts by weight of soil may include about 20 parts by weight or less of the polymer viscous biopolymer, but may not be limited thereto. For example, the polymer viscous biopolymer may be about 0.00001 parts by weight to about 15 parts by weight, about 0.00001 parts by weight to about 10 parts by weight, about 0.00001 parts by weight to about 5 parts by weight, based on about 100 parts by weight of soil. 0.00001 parts by weight to about 1 part by weight, about 0.00001 parts by weight to about 0.5 parts by weight, about 0.00001 parts by weight to about 0.1 parts by weight, about 0.0001 parts by weight to about 20 parts by weight, about 0.01 parts by weight to about 20 parts by weight, about 0.05 parts by weight to about 20 parts by weight, about 0.1 parts by weight to about 20 parts by weight, about 0.5 parts by weight to about 20 parts by weight, about 1 part by weight to about 20 parts by weight, about 5 parts by weight to about 20 parts by weight, or About 10 parts by weight to about 20 parts by weight, but may not be limited thereto.

The vegetation-promoting soil composition according to the second aspect of the present application, the detailed description of the overlapping parts of the first aspect of the present application, but the description of the first aspect of the present application is that in the second aspect of the present application Although the description is omitted, the same may be applied.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the following Examples are only provided to help understanding of the present application, and the contents of the present application are not limited to the following Examples.

[Example]

Here, various indoor experiments were conducted to verify the vegetation enhancing effect of the biopolymer. As the polymer chain-type biopolymer material, beta-1,3 / 1,6-glucan-based liquid product (glucan) was used, wherein betaglucan is a glycoside bond of two or more glucose molecules (D-glucose). It is a polysaccharide made of polymer by (glycosidic bonding). As a gelation polymer, xanthan gum (Sigma-Aldrich; CAS 1138-6-2) in a pure powder state, which is widely used as a food curing agent, was used. Here, xanthan gum is D-glucuronic acid (D-glucuronic acid), D-mannose (D-mannose), pyruvylated mannose, 6-O-acetyl-D-mannose (6-O-acetyl-D -mannose), and an anionic biopolymer consisting of 1,4-linked-glucan (Cadmus et al., 1982, "Biodegradation of Xanthan gum by Bacillus Sp", Applied and Environmental Microbiology , Vol. 44, No. 1, pp. 5-11].

Example 1: Biopolymer  Used Vegetation  Promotion effect measurement

In this example, various indoor experiments were conducted to verify the vegetation enhancement effect of the biopolymer. In this embodiment, beta-1,3 / 1,6-glucan-based liquid product (glucan Co., Ltd.) was used as the polymer chain biopolymer material. In addition, as a gelation polymer, xanthan gum (Sigma-Aldrich; CAS 1138-66-2) in a pure powder state, which is widely used as a food curing agent, was applied to this example.

The basic method for carrying out the invention is to mix the soil with the corresponding biopolymer, sowing crops and cultivating under constant temperature and humidity conditions to check the germination and growth of seeds, further analyzing the structure of the soil and how the biopolymer treated soil is planted. It was confirmed whether it affects the growth of. The details are as follows.

1. Seed Germination for Biopolymer Treated Soil

In the present embodiment, a granite residue (ocher), which is a main component of Halloysite: Al ₂ Si ₂ O ₅ (OH) ₄ , which is a representative soil of Korea, was used as a representative soil sample. After the natural drying, the soil was ground to a size of about 0.07 mm to about 0.15 mm, and then dried at 110 ° C. to remove residual organic matter.

Six pots were prepared and three (A, B, and C) were filled with basic soil, and the remaining three (D, E, and F) were artificially commercially available. A and D are pure soil conditions without any treatment, B and E are mixed with Xanthan gum, which is equivalent to 1% of soil weight. Finally, C and F are beta equivalent to 0.5% of soil weight. Glucan (Beta-glucan) was mixed. On top of that, about 600 seeds of oat were evenly sown with test crops, and then covered. A, B, C, D, E, F were all sprinkled with water so that the initial water content (amount of water to soil weight) was 60% and placed in a greenhouse at the same temperature and sunshine conditions (see FIG. 5). Figure 5 shows a test cultivation photograph of the biopolymer treated soil according to this embodiment. Germination and growth trends were observed daily, and the same amount of water was supplied to each pot when watering.

Seed germination results according to the cultivation day of each pot is shown in Table 1.

As a result, it was confirmed that seed germination was promoted in biopolymer-treated soil in both loess and cultured soil. Among the biopolymers, beta glucan was more efficient than xanthan gum. In particular, when the germination of the cultured soil was better than that of the loess, the germination of ocher (C) treated with betaglucan showed better results than that of the cultured soil (D) without any treatment. Improvement could be confirmed.

2. Plant Growth for Biopolymer Treated Soil

In the above example, seed germination was observed and growth of the entire vegetation was observed. As a method of observation, each pollen was divided into 8 zones, and the average growth length of the vegetation was measured in each zone to obtain the average of the whole. The growth results according to the cultivation day of each pot are shown in Table 2.

In relation to the cultivation results, Figure 6 shows the vegetation growth results with time of the biopolymer treated soil according to this embodiment. As shown in Figure 6, it was confirmed that the growth of vegetation in the soil treated with the biopolymer in both ocher and cultured soil. Among the biopolymers, beta glucan had a better effect than xanthan gum. In the case of ocher, it was confirmed that plant growth was promoted up to 5 times in beta glucan treated soil in the early stage (day 0 to 12). In particular, the growth of beta glucan-treated ocher (C) showed similar trends in the growth of untreated soil (D), indicating that beta-glucan treatment significantly improved soil performance not suitable for plant growth. there was.

3. Microstructure Analysis of Biopolymer Treated Soil

In order to determine how the biopolymers interact with soils in soil, they observed the mutual behavior of soil-biopolymer-vegetation roots using an electronic projection microscope (SEM, Philips XL30SFEG). The results are shown in Figures 7a to 7c.

Figure 7a is an electron projection microscope picture taken untreated ocher and vegetation roots according to this embodiment, Figure 7b is an electron projection microscope picture taken beta glucan treated ocher and vegetation roots according to the present embodiment , FIG. 7c shows an electron projection microscope photograph of xanthan gum treated loess and vegetation roots according to the present embodiment. In Figure 7a it can be seen that the ocher particles and vegetation roots are very tightly bonded as a result of the ocher untreated. In the case of beta glucan treated ocher of FIG. 7b, it is determined that the polymer beta glucan chains have an effect of improving the air permeability and moisture permeability of the soil by expanding the pores in the soil. On the other hand, in the case of xanthan gum treated soil (FIG. 7C), the overall soil structure is denser than that of the beta glucan treated soil of FIG. 7B, but due to gelation, soil particles form agglomerates and loosen than the untreated soil of FIG. 7A. Showing structure was observed. Therefore, it could be confirmed that the biopolymer treatment broadens the pores of the soil to create an environment in which the roots of the vegetation can grow well.

4. Verification of water retention performance of biopolymer treated soil

Vegetation growth and soil water content are closely related. The longer soil moisture content is maintained, the better the initial growth of the plant. Therefore, experiments were conducted to compare the water retention characteristics of biopolymer treated soil and soil.

Three samples of the same amount (200 g) of ocher were prepared, and the initial water content was changed to 60% of betaglucan treatment, 0.5% of soil weight, xanthan gum treatment, and no treatment. Matched in% and dried at room temperature. The weight of the specimen was weighed over time to determine the amount of water lost (evaporated). Evaporation rate over time [%; Initial water content (120 g)] The results are shown in Table 3.

According to Table 3, all of the biopolymers according to the present application can be confirmed to maintain a good water content in the soil compared to the soil without any treatment. In the early stages, the moisture of the soil surface evaporated mainly, so the difference between the biopolymer treated soil and the untreated soil was not large.However, in the medium and long-term behavior in which water in the soil is lost, the biopolymer treatment has an excellent effect on suppressing moisture loss in the soil. I could confirm it.

It will be understood by those of ordinary skill in the art that the foregoing description of the embodiments is for illustrative purposes and that those skilled in the art can easily modify the invention without departing from the spirit or essential characteristics of the present invention. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims rather than the detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention .

Claims (17)

A method for promoting germination or growth of vegetation, comprising adding a polymeric viscous biopolymer to the soil.
The method according to claim 1,
The polymer viscous biopolymer comprises a polysaccharide-based or amino acid-based biopolymer, a method for promoting germination or growth of vegetation.
3. The method of claim 2,
The polysaccharide-based polymer viscous biopolymer comprises a polymer chain biopolymer or a gelled biopolymer, a method for promoting germination or growth of vegetation.
The method according to claim 1,
The polymer viscous biopolymer includes a substance having glucose as a monomer, and includes beta glucan, alpha glucan, xantham gum, gellan gum, wellan, agar, succinoglycan, curdlan, and A method for promoting germination or growth of vegetation, comprising one selected from the group consisting of combinations thereof.
The method according to claim 1,
Wherein said polymeric viscous biopolymer comprises one selected from the group consisting of chitosan, gamma fiji (γPGA), and combinations thereof.
The method according to claim 1,
A method for promoting germination or growth of vegetation, comprising adding the polymer viscous biopolymer to 20 parts by weight or less based on 100 parts by weight of soil.
The method according to claim 1,
The polymer viscous biopolymer is to expand the pores in the soil, to maintain the soil water properties, and to increase the bonding strength between the soil particles, the method of germination or growth of vegetation.
The method according to claim 1,
The addition of the polymer viscous biopolymer to the soil may be performed by mixing the polymer viscous biopolymer with the soil, spraying the surface of the soil, or injecting the soil into the soil. Way.
The method according to claim 1,
A method for promoting germination or growth of vegetation, comprising adding the polymer viscous biopolymer to soil in an aqueous or basic aqueous solution.
The method according to claim 1,
Comprising the step of adding the polymer viscous biopolymer to the soil in a powder state, germination or vegetation enhancement method of vegetation.
The method according to claim 1,
After adding the polymer viscous biopolymer to the soil, further comprising adding a cation of an alkali metal or alkaline earth metal, germination or growth promotion method of vegetation.
The method according to claim 1,
After adding the polymer viscous biopolymer to the soil, further comprising adding an acidic aqueous solution or cationic aqueous solution, the germination or growth promotion method of vegetation.
The method according to claim 1,
After adding the polymer viscous biopolymer to the soil, and further comprising heating and cooling, vegetation germination or growth method.
14. The method of claim 13,
After the cooling, further comprising adding a cation of an alkali metal or an alkaline earth metal.
The method of claim 8,
After spraying the polymer viscous biopolymer on the surface of the soil, further comprising spraying water and / or acidic aqueous solution, the germination or growth promotion method of vegetation.
A soil composition for promoting germination or growth of vegetation, which is prepared by the method for promoting germination or growth of vegetation according to any one of claims 1 to 15, comprising a polymer viscous biopolymer.
17. The method of claim 16,
Containing 20 parts by weight or less of the polymer viscous biopolymer with respect to 100 parts by weight of soil, vegetation germination or growth promoting soil composition.

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