KR20140044078A - A method for preparing the essential oil fraction showing potent anti-obesity and inhibiting activity on fatty liver from angelica gigas nakai and the composition comprising the same the prevention or treatment of obesity and fatty liver - Google Patents

Abstract

The present invention relates to a production method of an essential oil fraction showing fatty liver control and anti-obesity activity from Angelica gigas Nakai, and a composition having prevention and treatment effects on fatty liver and obesity containing the fraction. The composition of the present invention shows body weight and body fat reduction, blood triglyceride reduction, and intake food amount reduction in obesity induced animals through a high fat diet; and is confirmed to have effects of controlling fat hepatopathy, fat cell hypertrophy, pancreatic islet increase, and the increase of insulin and glucagon producing cells, thereby capable of being practically used for medicines and health functional food for preventing and treating fatty liver and obesity.

Description

FIELD OF THE INVENTION The present invention relates to a process for preparing an essential oil fraction exhibiting anti-obesity activity and a composition having the effect of preventing and treating obesity and fatty liver including the fraction, fatty liver from Angelica gigas Nakai and the composition comprising the same or prevention of obesity and fatty liver.

The present invention relates to a process for producing an essential oil fraction exhibiting an anti-obesity activity, and a composition for preventing and treating obesity and fatty liver comprising the fraction.

[Document 1] Van der Ploeg LH. Related Obesity: an epidemic in need of therapeutics. Curr Opin Chem Biol. 4 (4) , pp. 452-460, 2004

[Literature 2] Aviva M, et al. The disease burden associated with overweight and obesity. JAMA, 282 , pp. 1523-9, 1999

[3] Park SH, Jeon WK, Kim SH, Kim HJ, Park DI, Cho YK, Sohn IK, Sohn CI, Keum DK, Kim BI. Prevalence and risk factors of non-alcoholic fatty liver disease among Korean adults. J Gastroenterol Hepatol 21 : 138-143, 2006.

[4] Asselah T, Rubbia-Brandt L, Marcellin P, Negro F. Steatosis in chronic hepatitis C: why does it really matter? Gut 55: 123-130, 2006.

[Literature 5] Jung. B. The annual socioeconomic costs of six obesity-related diseases. Unpublished master's dissertation, Seoul National University, Seoul. 2001

[Document 6] Kim Eun-sun et al. 2, Obesity Therapeutics, In-depth Information Analysis Report, Korea Institute of Science and Technology Information, pp.1-111, 2002

[Literature 7] Lee, GJ et al. 1, Predisposition of waist circumference and obesity in women. Korean Journal of Obesity, 5 , pp.41-48, 1996

[Literature 8] Treatment of obesity. Korean Journal of Nutrition, 5 (2) , pp.179-190, 1990

[Literature 9] Lee, Jonghwa et al. 1, Life change after menopause and acceptance of it in menopausal women. Korean Journal of Menopause, 9 (3) , pp.254-260, 2003

[Literature 10] Shin, Jin Hee. A survey on utilization of aromatherapy by skin care workers, Thesis of master 's degree, Kosin University. 2000

[Literature 11] Korea Aroma Association. Aromatic Essential Oil Encyclopedia. Hyunmosa, Seoul, 2002

[12] Park, Jin-A, Volatile Compounds and Flavor Components in Angelica Gigantis Radix, Master's thesis, University of Ulsan. 2008

[Literature 13] Kim Hee Ja. Effects of aromatherapy massage on body composition and serum lipids in obese women after menopause, Journal of the Korean Nursing Society for Geriatrics, 9 (1) , pp.29-39, 2007

[14] Kim, Myoung Sook1, Effects of characteristics and obesity management on middle-aged abdominal obesity women, Korean Journal of Obesity, 12 (1) , pp.65-66, 2003

[15] Han SK, Effects of Angelica gigas Nakai herbal acupuncture into Hyolhae (SP10) of brain ischemic injury induced by Intraluminal filament insertion in the rats, The Journal of Korean Acupuncture & Moxibustion Society, 21 (2) 20, 2004

[0008] Huang B. et al., Cinnamaldehyde inhibits adipocyte differentiation and adipogenesis via regulation of peroxisome proliferator-activated receptor-γ (PPARγ) and AMP-activated protein kinase (AMPK) pathways, J Agric Food Chem, Apr 27 (8) , pp.3666-73, 2011

[17] Allain C. et al., Enzymatic determination of total serum cholesterol, Clin. Chem. 20 (4) , pp.470-475, 1974

Food and Chemical Toxicology, 44 (11) , pp. 1875-1883, 2006). In addition, the lipid profiles of lipids, .

[Literature 19] Allain C. et al., Enzymatic determination of total serum cholesterol, Clin. Chem. 20 (4) , pp.470-475, 1974

[20] Van Handel E. et al., Micromethod for the direct determination of serum triglycerides, J. Lab. Clin. Med. 50 (1) , pp.152-157, 1957

[21] Giusti G. et al., A comparative study of some spectrophotometric and colorimetric procedures for determination of serum glutamic-oxaloacetic and glutamic-pyruvic transaminase in hepatic diseases. Enzymol. Biol. Clin (Basel), 10 (1) , pp. 17-38, 1969

[22] Murase T. et al, Nootkatone, a characteristic constituent of grapefruit, stimulated energy metabolism and prevents diet-induced obesity by activating AMPK, Am J Physiol Endocrinol Metab, 299 (2) , pp.E266-75, 2010

[PubMed 23] Huh, Myung-Sun 4 and others, Effects of incense-sucking on body weight, dietary intake, intake efficiency and serum leptin in obesity-induced rats, Journal of the Korean Society for Plants and Environment ,

[PubMed 24] Koo Sekwang et al. 3, Anti-obesity effect of acid garlic leaf extract in obese mice induced by high fat diet, Korean Journal of Veterinary Clinics, 28 (3) , pp.280-286, 2011

Obesity is defined as the excess accumulation of body fat. Obesity is a state of excessive accumulation of body fat, which is harmful to health. It can be defined as obesity when there is an increase of more than 20% from the standard weight of each individual. Specifically, the percentage of body fat in the weight of the fat is 25% or more in males and 30% or more in females (Van der Ploeg LH). 4 (4) , pp. 452-460, 2004). This is one of the serious health problems that are soaring not only in Western society but also in many Asian countries including Korea. If the current global trend continues, one-third of the world's population will become obese (BMI, Body Mass Index, 30 kg / ㎡ or more) by 2025, the International Obesity Task Force .

Obesity is known to cause various diseases such as type 2 diabetes, hypertension and coronary artery disease as well as cosmetic stress, and it is known to increase the incidence of breast cancer, endometrial cancer, prostate cancer and colorectal cancer (Aviva M, et , al., The disease burden associated with overweight and obesity. JAMA, 282 , pp. 1523-9, 1999).

Liver plays an important role in regulating liver fat accumulation and insulin resistance through the balance of fatty acid synthesis and oxidation, and maintaining metabolic homeostasis in the body. Non-alcoholic fatty liver disease (NAFLD), which causes excessive accumulation of triglycerides in the liver, is associated with simple hepatic steatosis, steatohepatitis, hepatic fibrosis, And hepatocellular carcinoma (HCC), as well as chronic liver disease such as liver cancer (Park SH, Kim SH, Kim HJ, Park DI, Cho YK, Sung IK, Sohn CI, Keum DK, Kim BI. J Gastroenterol Hepatol 21 : 138-143, 2006). Recently, it has been known to be associated with metabolic syndrome such as obesity, insulin resistance, type 2 diabetes and cardiovascular disease (Asselah T, Rubbia-Brandt L, Marcellin P, Negro F. Steatosis in chronic hepatitis C: why does it really matter Gut 55: 123-130, 2006).

In addition, obesity has been reported as accounting for the socio-economic costs of six obesity-related diseases at 2.1-1.1% of the total national health expenditure, and the economic burden is also large (Jung, related disease. Unpublished master's dissertation, Seoul National University, Seoul, 2001).

Obesity is a problem in the frequency of occurrence, related diseases, economic loss, and everyday life. In modern society, intervention for obesity should be done through more multidimensional and scientific methods, and active prevention is needed .

Obesity treatment methods include the use of appetite suppressants, fat absorption inhibitors, energy metabolism accelerators, hormone preparations, and other surgical procedures such as gastrectomy and liposuction. However, (Kim et al., 2, Obesity treatment, In-depth information analysis, Korea Institute of Science and Technology Information, pp.1-111, 2002).

There are several ways to manage obesity: diet, exercise, pharmacotherapy, surgery, etc. However, there is a side effect that the visceral fat reduction rate is lower and the visceral fat is increased more when the diet is returned to the original diet and 1, the waist hip circumference ratio and obesity-related disease prediction in women. Journal of obesity, 5, pp.41-48, 1996) exercise therapy is not only effective but far excel time in a long economic aspects of modern life hanging (2) , pp. 179-178. In addition, it is reported that there is a risk of economic burden, pain, side effects, 190, 1990). In fact, 44% of post-menopausal women in Korea are using alternative therapies such as massage, sauna, and jjimjilbang in order to lose weight. 9 (3) , pp. 254-260, 2003). Of the people using alternative therapies, 25% had obesity management (Kim, Jin-hee, A survey on utilization of aromatherapy by skin care workers, Kosin University, Master's thesis, 2000).

Aromatherapy is a combination of aroma, which means fragrance and aroma, and therapy, which means therapy. It is a combination of all the various parts extracted from seeds, shells, roots, leaves, flowers, trees, balsam and resin Korea Aroma Association, Encyclopedia of Aroma Essential Oil, Hyunmunsa, Seoul, 2002) Maintaining and promoting health by improving incompatibility of body and mind by absorbing essential oil components that emit volatile and unique fragrance through the olfactory or skin (Kim, Myung-Sook et al., 1, Characteristics of obese middle-aged women and effect of obesity management, Korean Journal of Obesity, 12 (1) , pp.65-66, 2003). The fragrance therapy includes massage method, diffusing method to induce incense, inhalation method to inhale nostril, bathing method, puffing method to freshen painful area, gagging method to rinse mouth, dosing method and application method according to the method of administration. The absorption pathway through the nose is absorbed into the nose and the olfactory nerves in the mucous membrane transmit the cerebrum to the cerebrum, which is analyzed in the limbic system of the cerebrum, and then the neurochemical substance is controlled to stabilize the imbalance of mind and body. These neurochemicals also affect the pituitary gland and affect the whole body tissue by regulating hormone secretion.

The fragrant Angelica Gigantis Radix is a perennial herbaceous plant belonging to the persimmon family, which means one to two years old, and it is rooted in Korea. It is collected late in autumn to remove the whisker root, wait for a little evaporation of water, and slowly fumble with smoke. The active ingredients of Angelica gigas include decursin of coumarin, ligustilide of decousinol, phthalide, butylidene phthalide, and butylphthalide. Roots contain verapathecent C12H8O4, isopimpineline, about 0.2% essential oil, and β-sitosterol.

As one of the measures to solve obesity, aromatherapy has been recently popularized, and there are more than 70 kinds of essential oils currently used. However, scientific studies on aromatic essential oils targeting obesity are very rare. Oriental oriental ginseng has been extensively studied, but research on ginseng essential oil in the treatment of obesity has not been active in Korea and abroad.

Accordingly, the present inventors have researched the above problems and developed a therapeutic agent effective for obesity. Through the studies to develop an effective therapeutic agent for obesity, the inhalation of the fractions of Angelica gigas Oviposus was reduced in body fat and body fat, . In addition, the present invention has been completed by confirming that the hyperlipidemia induced by feeding the high fat tissue in the tissues significantly inhibits the increase of the hepatopathy, fat cell hypertrophy, pancreatic islet hyperplasia, insulin, glucagon producing cell.

In order to attain the above object, the present invention provides a method for producing Angelica gigas Nakai, which comprises drying and crushing Angelica gigas and then washing the Angelica gigas with a volume of about 1 to 100 times, preferably about 2 to 20 times the volume of the sample in n-hexane, heptane, methylene chloride, Acetate, preferably hexane or ethyl acetate, more preferably a hexane solvent, at 20 to 120 ° C, preferably at room temperature to 80 ° C for about 24 hours to 144 hours, preferably 48 to 120 hours, The extract obtained by extracting with a cold extraction method is preferably used at a temperature of 20 to 80 占 폚, preferably 30 to 60 占 폚, for a period of 96 hours by using an extraction method such as a cold extraction, a hot water extraction, a cold extraction, a reflux cooling extraction or an ultrasonic extraction Filtration and concentration under reduced pressure to obtain an Angelica nonpolar solvent fraction; Ethanol is added to the non-polar solvent fraction at a rate of about 0.05 to 50 times, preferably 0.5 to 5 times (v / w%) the weight of the fraction, and the resulting fraction is concentrated to concentrate the second fraction To obtain a fraction of Angelica gigas Fern oil having anti-obesity activity.

The present invention also provides a pharmaceutical composition and a health functional food for the treatment or prevention of fatty liver and obesity, which comprises the above-described production method and the fraction of Angelica gigas Fertilization obtained by the above-mentioned manufacturing process as an active ingredient.

The above-obtained dietary fractions of Angelica gigas Fertifolia have resulted in reduction of body weight and body fat, decrease of blood triglyceride concentration and decrease of dietary intake in obesity-induced animals using high-fat diets. In addition, significant inhibition of lipid peroxidation, adipocyte hyperplasia, pancreatic islet hyperplasia, insulin and glucagon - producing cells induced by high - fat diet in the tissues was significantly confirmed.

The pharmaceutical composition for treating fatty liver and obesity of the present invention comprises 0.01 to 80% by weight, preferably 10 to 50% by weight, of the fraction based on the total weight of the composition.

The pharmaceutical compositions comprising the fractions of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions.

The pharmaceutical dosage forms of the fractions of the present invention may be used in the form of their pharmaceutically acceptable salts and may also be used alone or in combination with other pharmaceutically active compounds as well as in suitable aggregates.

The pharmaceutical composition containing the fractions according to the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations such as external preparations, suppositories and sterilized injection solutions, Can be used. Carriers, excipients and diluents that may be included in the composition comprising the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, weights, binders, wetting agents, sealing agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate, sucrose, and the like in the extracts and fractions. (sucrose), lactose (lactose), gelatin, etc. are mixed and prepared. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories.

It is preferable that the formulation containing the essential oil fraction extracted and isolated from Angelica gigas of the present invention is administered by parenteral administration, for example, in the form of a spray in the form of an inhalation, or a non-forced agent.

Accordingly, the present invention provides a spray formulation or a non-hardening agent for the treatment and prevention of fatty liver and obesity, which comprises the fraction of Angelica gigas Fertilization oil produced by the above production method as an active ingredient.

In the above formulations, non-aqueous solvents and suspending agents may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like. As a suppository base, witepsol, macrogol, tween 61, cacao paper, laurin, glycerol gelatin and the like can be used.

The preferred dosage of the pharmaceutical composition comprising the fractions of the present invention will vary depending on the condition and weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the pharmaceutical composition containing the fraction of the present invention is preferably administered at 0.0001 to 100 mg / kg per day, preferably 0.001 to 100 mg / kg per day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The fraction of Angelica gigasini oil of the present invention can be administered to mammals such as rats, mice, livestock, and humans in various routes. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or Intracerebroventricular injection.

The present invention also provides a health functional food for preventing or ameliorating fatty liver and obesity, which comprises the fraction of Angelica gigas Fertilized oil produced by the above production method having an excellent inhibitory effect on obesity as an active ingredient.

&Quot; Health functional food "as defined herein means food prepared and processed using raw materials or ingredients having functionality useful to the human body in accordance with Law No. 6727 on Health Functional Foods." Functional " Structure and function of the nutrient to control or physiological effects, such as to obtain a beneficial effect for health is intended to eat.

The health functional food for preventing or ameliorating fatty liver and obesity of the present invention contains 0.01 to 95% by weight, preferably 1 to 80% by weight, of the fraction based on the total weight of the composition.

For the purpose of preventing or ameliorating fatty liver and obesity, a pharmaceutical dosage form such as powders, granules, tablets, capsules, pills, suspensions, emulsions and syrups, or a health functional food in the form of tea bags, Manufacturing and processing are possible.

In addition, the present invention provides a health supplement containing as a main component a fraction of Angelica gigas Fertilis that is produced by the above-described method showing fatty liver and obesity-suppressing activity.

In addition, the present invention provides a food additive containing as a main ingredient a fraction of Angelica gigas Fertilis that is produced by the above-described method showing fatty liver and obesity-suppressing activity.

The health functional beverage composition of the present invention is not particularly limited to other components other than those containing the above-mentioned fraction as essential components in the indicated ratios and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. As natural flavors other than those described above, natural flavors (such as tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin)) and synthetic flavors (saccharin, aspartame, etc.) have. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above-mentioned composition, the composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and intermediates (cheese, chocolate etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. In addition, the compositions of the present invention may contain flesh for the production of natural fruit juices and fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

In addition, the fraction of the present invention can be added to foods or beverages for the purpose of preventing fatty liver and obesity. At this time, the amount of the fraction in the food or drink may be 0.01 to 15% by weight of the total food, and the health beverage composition may be added in a proportion of 0.02 to 5 g, preferably 0.3 to 1 g, .

The essential oil fraction extracted from Angelica gigas Nakai of the present invention is useful as a pharmaceutical composition for the prevention and treatment of fatty liver and obesity or a functional product for inhalation, as well as reducing weight loss, body fat reduction and blood lipid concentration in obesity- Health functional foods and health supplements.

FIG. 1 is a graph showing the effect of the fractions of Angelica keiskei kanseri on body weight of obese rats due to high fat diet administration,
FIG. 2 is a graph showing the effect of the fractions of Angelica keiskei L. on the dietary intake of obese rats due to high fat diet administration,
FIG. 3 is a graph showing the effect of the fractions of Angelica keiskei L. on the liver weight of obese rats due to administration of high fat diet,
FIG. 4 is a graph showing the effect of the Angelica keiskei koidz oil fraction on body weight of obese rats due to administration of (ad) high fat diet,
FIG. 5 (ad) is a graph showing the effect of the Angelica keiskei koreana fraction on the serum liver function and triglyceride concentration in obese rats fed with high fat diet,
6 (ah) is a graph showing the effect of the Angelica keiskei kansen fraction on hepatocyte hypertrophy and hepatic hyperplasia across the whole hepatic lobule of obese rats due to administration of high fat diet,
FIG. 7 (ah) shows the effect of the Angelica keiskei korean oil fraction on epididymal fat cell hypertrophy in the epididymal tissues of obese rats due to administration of the high fat diet,
8 (ah) is a graph showing the effect of the Angelica keiskei koreana fraction on the distribution of pancreatic islets in combination with the reduction of pancreatic Zymogen granules in obese rats due to the high fat diet administration,
9 (ah) shows the effect of the fractions of Angelica keiskei kansei on the number increase of insulin-producing cells in obese rats due to administration of high fat diet,
Fig. 10 (ah) shows the effect of the Angelica keizan extract fraction on the increase in number of glucagon-producing cells in obese rats due to administration of the high fat diet.

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following Examples, Reference Examples and Experimental Examples.

Example  1. Angelica oil Fraction  Produce

8 kg of Dengue was purchased from Daewon Pharmaceutical Co., Daegu, and cleaned, ground, put into an extractor, added with 30 L of n-hexane, and then stirred for 72 hours at room temperature while being stirred. The extracted liquid was extracted with a rotary vacuum distiller 220, Buchi, rotary vacuum evaporator, Switzerland). Ethanol (ethanol 10L) was added to the concentrated Angelica giganteus extract and re-dissolved. The precipitated wax layer and the like were filtered (Filter Paper NO2 Advantec) and concentrated to obtain essential components used in the experiment. As a result, the yield of the obtained essential oil was about 1.81% (hereinafter referred to as AGEO).

Reference example  1. Preparation of experimental animals

Experimental animals were ICR mice (Orient) at 5 weeks of age, weighing 28 ~ 32g. The animals were maintained in a constant temperature condition (temperature: 21 ± 2, darkness: 12 hours) Free drinking of water, and sufficient water and food until the start of the experiment.

Reference example   2. Inhalation of Angelica essential oil and induction of obesity

To induce obesity, experiments were conducted using the dietary compositions described in the literature (Lee JS et al., Supplementation of whole persimmon leaf improves lipid profiles and suppresses body weight gain in rat fed high-fat diet, Food and Chemical). Toxicology, 44 (11) , pp. 1875-1883, 2006).

First, in the experimental group except the normal group, a diet based on the AIN-76 composition was prepared by adding a pellet-shaped solid feed (# 102038, AIN-76A Based High Fat / High Carb Purified Rodent Diet with Cholesterol and Cholic Acid, Dyet, USA) for 6 weeks, and 15 animals were assigned to each group. (NOR), high fat control group (HFD), high fat diets, and Angelica gigas oil inhalation group (AGEO) which inhaled the fraction of essential oil of Angelica gigas Nakai. The gangrene essential oil inhalation group (AGEO) was further subdivided into a 2 week inhalation group (2AGEO) and a 3 week inhalation group (3AGEO), and the gangwoe essential oil fraction obtained in Example 1 was administered for 12 hours every day for 4 weeks, , Or high fat diet was inhaled with high fat diet from 3 weeks after feeding to 3 weeks, and 10 g of feed was allocated daily.

Ingredient Normal HFD Casein 20 20 DL - Methionine 0.3 0.3 Com starch 15 15 Sucrose 50 39.75 Cellulose 5 5 Corn oil 5 5 Mineral-mix 3.5 3.5 Vitamin-mix One One Choline bitartrate 0.2 0.2 Lard - 10 Cholesterol - 0.2 Cholic acid - 0.05 total (%) 100 100 Kcal / 100g diet 3850 4340 Calorie from fat (%) 117 311 Calorie from carbohydrate (%) 675 505 Calorie from protein (%) 208 184

Reference Example 3. Statistics Processing

The statistical significance of the test results was analyzed using two-way ANOVA and LSD post-test of the SPSS program (Version 14.01) . 05 , and statistical significance (#) with NOR group and statistical significance with HFD group (*) were shown in the figures and tables, respectively.

Experimental Example 1. Dietary Efficacy, Body Weight and Tissue Harvesting in Obese-Induced Rats Using High-Fat Diet

In order to confirm the effects of the gangrene essential oil fraction (AGEO) of the above example on the dietary efficiency and body weight measurement of obesity-induced rats using a high fat diet, the following experiment was conducted by applying the method described in the literature Effects of Inhalation on Body Weight, Dietary Intake, Intake Efficiency and Serum Leptin in Obese Rats, Korean Journal of Human Ecology, Plant and Environment pp.113-124, 9 (2), 2006).

Dietary intake was measured daily at 5 pm, daily intake was calculated, and body weight was measured every 3 days. The food efficiency ratio (FER) is the body weight gain during the experiment and the value divided by the dietary intake (FER = body weight gain / food intake).

As shown in Table 2 and FIG. 1, after 3 weeks, the body weight of the high-fat diet increased significantly in the HFD group compared to the body weight of the normal group (NOR) In the main inhalation group (3AGEO), weight gain was significantly inhibited. In addition, as shown in Table 2 and Figure 2, the dietary intake was significantly reduced in the 2AGEO, 3AGEO group when compared to the normal group (NOR). The change of body weight was significantly decreased in the NOR group, 2AGEO and 3AGEO groups compared to the HFD group. Dietary efficiency (FER) was also significantly higher in the HFD group and the 3AGEO group compared to the NOR group.

On the other hand, the experimental animals were fasted for 12 hours before sacrifice and then harvested with visceral adipose tissue (brown adipose tissue) and brown adipose tissue (BAT) by liver and part under anesthesia with pentobarbital Respectively. The tissues for tissue staining were fixed in formalin solution, and the body fat tissues for analysis were stored at -80 ° C until they were rapidly frozen in liquid nitrogen.

According to the results of FIG. 3 and FIG. 4 (ad), liver weight, epididymal fat mass, internal weight and brown fat (BAT) weight were significantly increased in the high fat dietary group (HFD) The epididymal fat mass, the weight of the fat around the kidney, the internal weight and the brown fat weight were decreased in the group, and 2AGEO group was significantly inhibited. This result shows that the effect of the strong Angelica gigantis essential oil on the reduction of body fat, which is known to cause complications such as cardiovascular diseases rather than simple weight loss in obesity, is more meaningful as a therapeutic agent for obesity.

NOR HFD 2AGEO 3AGEO Food intake 5.14 ± 0.43 4.33 ± 0.83 4.48 + - 0.54 3.62 ± 0.40 Weight gain (g / day) 0.17 + 0.07 0.22 ± 0.04 0.01 ± 0.05 0.06 ± 0.07 FER 0.03 0.02 0.05 ± 0.01 0.02 ± 0.01 0.04 0.02

Experimental Example  2. Blood collection and serum analysis of obese rats

The animals were fasted for 12 hours before sacrifice and anesthetized with pentobarbital sodium (10901, Hanlim Pharmaceutical Co., Ltd., Korea), and blood was collected from the abdominal vena cava. The total blood cholesterol (TC) was measured by the enzyme method of C. Allain 1 (Allain 1), and the total cholesterol (TC) was measured by centrifugation at 4 ° C and 3,500 rpm for 15 minutes. (L-type GHO M, Wako, Japan) was used in accordance with the method described in K. et al., Enzymatic determination of total serum cholesterol, Clin. Chem. 20 (4) , pp.470-475, triglyceride content was determined by the enzyme method of Van Handel et al (Micromethod for the direct determination of serum triglycerides, J. Lab. Clin. Med. 50 (1) , pp. 152-157, 1957) (L-type TG M, Wako, Japan) was used.

Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) contents, which are transaminases related to liver function, were determined by spectrophotometry (Giusti G. et al., A comparative study of some spectrophotometric and (L-type ALT) using an enzyme-linked immunosorbent assay (ELISA ) for the determination of serum glutamic-oxaloacetic and glutamic-pyruvic transaminase in hepatic diseases. Enzymol. Biol. (Hitachi 7080, Japan) using an automated biochemical analyzer (J2, L-type AST J2, Wako, Japan).

As shown in FIG. 5 (a), ALT (143.4 ± 7.8 U / L), AST (1238.8 ± 691.1 U / L) and triglyceride were significantly higher in the high fat diet group (79.4 ± 6.8, 71.4 ± 6.9 U / L) and AST (703.7 ± 265.0, 499.1 ± 137.8 U / L, respectively) in the 2AGEO and 3AGEO groups, respectively, L) and triglyceride (27.3 ± 7.6, 41.2 ± 13.4 ㎎ / ㎗) were decreased.

Experimental Example  3. High Fat Diet  Histopathological analysis of obesity-induced rats

In order to confirm the effect of AGEO on the obesity-induced rats in the above example, the following experiment was conducted by applying the method described in the literature (Sekwang et al., 3, Antiobesity effect of acid garlic leaf extract, Korean Journal of Veterinary Clinics, 28 (3) , pp.280-286, 2011)

MATERIALS AND METHODS : The hepatic, epididymal, and pancreatic parenchyma tissues were fixed in 10% neutral formalin in the normal control, HFD control, 2AGEO, and 3AGEO groups and dehydrated and paraffin- (MHS16-SIGMA) or Masson's trichrome (B8563, SIGMA) staining was performed under the light microscope. Insulin and glucagon-producing cells in the pancreatic islets were stained with avidin-biotin-peroxidase . (% / Mm ² of hepatic parenchyma), hepatocyte diameter (μm / hepatocyte), epididymal adipocyte cell size (μm / adipocyte) and pancreatic zymogen granule (% / mm ² ) for observation of clear histopathologic changes ² of exocrine pancreatic parenchyma) and the area occupied by pancreatic islets (% / mm ² of pancreatic parenchyma), insulin / glucagon cell ratio (cells / mm ² of pancreatic parenchyma) Were evaluated histomorphometrically using DMI-300 Image Processing (DMI, Korea) in each microscope field.

As shown in FIG. 6 (a-h), the fatty change was characterized by hypertrophy and vacuolization of hepatocytes throughout the liver lobe by high fat feed (HFD). On the other hand, in the 2AGEO and 3AGEO groups, in which the high-fat diet and the essential oil fraction were inhaled together, the hepatic artery induced by HFD supply was remarkably improved in both the 2AGEO and 3AGEO groups by decreasing the remarkable hepatocyte diameter as compared with the HFD control group. Significant diameter reduction was observed.

As shown in FIG. 7, the diameters of epididymal adipocytes increased dramatically due to the feeding of high-fat diets. However, in the 2AGEO and 3AGEO groups, in which the high fat diet and the fractionated fraction were inhaled, the diameter of epididymal adipocytes was significantly Respectively.

As shown in FIG. 8, in the HFD group, the pancreatic islet granules and the pancreatic islets were increased in the HFD group. However, in the 2AGEO and 3AGEO groups, The number of pancreatic endocrine hormones was normalized in both groups.

As shown in FIG. 9, endocrine disorders such as increase of insulin and glucagon producing cells were observed in the HFD group due to feeding of high fat milk. However, in the 2AGEO and 3AGEO group in which the high fat diet and the fractionated fraction were inhaled, It was remarkably normalized.

As shown in FIG. 10, endocrine disorders such as increase in glucagon-producing cells were observed in the HFD group due to the feeding of high fat milk. However, in the 2AGEO and 3AGEO group in which the high fat diet and the fractionated fraction were inhaled, It was normalized.

In the HFD control group, there was a significant (p <0.01) ratio of denatured liver, mean hepatocyte diameter, epididymal adipocyte size, pancreatic islet area, insulin and glucagon producing cells And pancreatic zymogen granules accounted for a significant increase (p <0.01). On the other hand, both 2AGEO and 3AGEO groups significantly inhibited the hepatopathy, fat cell hypertrophy, pancreatic islet hyperplasia, insulin and glucagon production cells induced by administration of high fat diet, In addition, the inhibition of the decrease of zymogen granule was observed, and it was judged that various changes caused by the high fat dietary supply were suppressed by controlling the lipid absorption and metabolism through their secretion regulation. Overall, , Fat cell hypertrophy, pancreatic islet hyperplasia, insulin and glucagon production.

Hereinafter, formulation examples of the composition containing the fractions of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation example  One. Sanje  Produce

Fractions of Angelicae essential oil (AGEO) --------------------------------- 20 mg

Lactose ------------------------------------------------- - 100 mg

Talc ------------------------------------------------- - 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

Formulation example  2. Preparation of tablets

Fractions of Angelica gigas oil (AGEO) --------------------------------- 10 mg

Corn starch -------------------------------------------- 100 mg

Lactose ------------------------------------------------- - 100 mg

Magnesium stearate ------------------------------------- 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

Formulation example  3. Preparation of capsules

Fractions of Angelica gigas oil (AGEO) --------------------------------- 10 mg

Crystalline cellulose --------------------------------------- 3 mg

Lactose --------------------------------------------- 14.8 mg

Magnesium stearate 0.2 mg &lt; RTI ID = 0.0 &gt;

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Formulation example  4. Preparation of injections

Fractions of Angelica gigas oil (AGEO) --------------------------------- 10 mg

Mannitol ------------------------------------------------ 180 mg

Sterile sterile distilled water used - 297 mg

Na ₂ HPO ₄ , 12H ₂ O ------------------------------------------- 26 mg

(2 ml) per ampoule in accordance with the usual injection method.

Formulation example  5. Liquid  Produce

Fractions of Angelicae essential oil (AGEO) --------------------------------- 20 mg

Isseong Party ------------------------------------------------ 10 g

Mannitol ------------------------------------------------- - 5 g

Purified water ------------------------------------------------- - Suitable amount

Each component was added to purified water in accordance with the usual liquid preparation method and dissolved, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was adjusted to 100 ml with purified water, And sterilized to prepare a liquid preparation.

Formulation example  6. Manufacture of spray formulations

Ingredients and Content (in 100 ml) (specific gravity: 0.812 g / ml)

Fractions of Angelica gigas (AGEO): 0.9 g

Glycerin: 6.30 g

Stevioside (100%): 0.020 g

Anhydrous ethanol: 74.8 g

6.30 g of glycerin, 70.0 g of anhydrous ethanol and 0.9 g of a fraction of Angelica ganoderma (AGEO) were first mixed and dissolved in the above raw material preparation, 0.02 g of stevioside (100% purity) Was added to the solution, and 4.8 g of anhydrous ethanol was added to make a total volume of 100 ml. The solution was put into a spray bottle and capped. Then, when using, the spraying device was replaced with a cap and was assembled with a spray agent do.

Formulation example  7. Manufacture of health food

Fractions of Angelica gigas (AGEO) --------------------------------- 1000 mg

Vitamin mixture ---------------------------------------------

Vitamin A Acetate -------------------------------------- 70 g

Vitamin E ------------------------------------------------ 1.0 mg

Vitamin B1 ---------------------------------------------- 0.13 mg

Vitamin B2 ---------------------------------------------- 0.15 mg

Vitamin B6 ----------------------------------------------- 0.5 Mg

Vitamin B12 ---------------------------------------------- 0.2 g

Vitamin C ------------------------------------------------ - 10 mg

Biotin ------------------------------------------------- - 10 [mu] g

Nicotinic acid amide 1.7 mg

Folic acid ------------------------------------------------- ---- 50 μg

Calcium pantothenate ------------------------------------------- 0.5 mg

Inorganic mixture ---------------------------------------------

Ferrous sulfate ---------------------------------------------- 1.75 mg

Zinc oxide ----------------------------------------------- 0.82 Mg

Magnesium carbonate - 25.3 mg

Potassium monophosphate ---------------------------------------------- 15 mg

Calcium Phosphate ---------------------------------------------- 55 mg

Potassium citrate ----------------------------------------------- 90 Mg

Calcium Carbonate ------------------------------------------------ 100 mg

Magnesium chloride ------------------------------------------- 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Formulation example  8. Manufacture of health drinks

Fractions of Angelicae essential oil (AGEO) --------------------------------- 1000㎎

Citric acid ------------------------------------------------ 1000 Mg

Oligosaccharide ------------------------------------------------ 100 g

Plum concentrate ------------------------------------------------ 2 g

Taurine ------------------------------------------------- --- 1 g

Purified water was added.

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated at 85 DEG C for about 1 hour with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, &Lt; / RTI &gt;

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

Claims (7)

After drying and grinding the Angelica vulgaris, a nonpolar solvent selected from n-hexane, heptane, methylene chloride, chloroform or ethyl acetate in a volume of 1 to 100 times the weight of the sample was added thereto, and the result was 24 to 144 hours at 20 to 120 ° C. A first step of extracting the extract obtained by cold extraction, hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction under reduced pressure filtration and concentration at 20 to 80 ° C. to obtain a donkey nonpolar solvent fraction; Fatty liver and anti-obesity were obtained from the Angelica mucus which comprises the second step by removing the precipitate produced by adding 0.05-50 times the volume (v / w%) of ethanol to the nonpolar solvent fraction, and concentrating the obtained essential oil fraction. A method for obtaining an essential oil fraction of Angelica gigas having activity. The method according to claim 1,
The non-polar solvent is a hexane or ethyl acetate solvent characterized in that the manufacturing method. The method according to claim 1,
The extraction method is characterized in that the cold extraction method. A pharmaceutical composition for the treatment or prevention of fatty liver and obesity, comprising the Angelica essential oil fraction obtained by the process of claim 1 as an active ingredient. The pharmaceutical composition of claim 4, wherein the pharmaceutical composition is a spray or nasal preparation. A health functional food for preventing or improving fatty liver and obesity, containing the Angelica essential oil fraction obtained by the process of claim 1 as an active ingredient. The health functional food according to claim 6, wherein the health functional food is in the form of powder, granule, tablet, capsule, pill, tea bag, leach tea or health beverage.

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