KR20140043202A - Drought stress resistant gene from potato and drought resistant transformant using the same - Google Patents

Abstract

The present invention relates to a drought stress resistant gene derived from a potato and a drought stress resistant transformant produced by using the same. The gene of the present invention is confirmed to be expressed from the potato with a low temperature stress resistance, and is capable of producing the transformant with drought stress resistance by transforming the genes, thereby capable of being practically used in developing plant bodies with resistance against dry environments. [Reference numerals] (AA) Collecting colonies; (BB) Separating plasmid; (CC) Transforming yeast and screening; (DD) Resistance test; (EE) Drying stress PEG (25%)

Description

Drought stress resistant gene from potato and drought resistant transformant using the same

The present invention relates to a gene derived from potato and having a resistance to dry stress, and a transformant having a dry stress resistance prepared using the same.

Potatoes ( Solanum tuberosum ) is a more crop crop per unit of arable land than corn, wheat, and rice. It is a major staple food resource that has spread worldwide since Columbus and is cultivated in most regions except the polar regions. In addition, more than half of the world's potato production comes from developing countries, where potatoes are an important part of food and farm income.

On the other hand, it is known that the productivity of crops is greatly reduced by environmentally stressed, such as extreme temperature, lack of moisture, excess salt, mineral deficiency and toxicity. Crop production loss due to abiotic environmental stress is huge, and it is confirmed that more than half of the possible yields are lost due to abiotic stress, and recently, a sharp change in the environment has been recorded locally. Is even more concerned. Pesticides are generally used to reduce the damage of crops and improve productivity due to environmental stress, but the use of pesticides not only causes serious problems such as environmental pollution, but also harmful effects on human body when ingested. However, the effect of reducing the production of agricultural products due to environmental stress has not been clearly identified as far as the fundamental mechanism of stress tolerance in plants. As for potato, biotechnology research has been very active, and it has shown remarkable achievements in research on late blight resistance genes, genetic map research using markers, quality improvement by changes in starch synthesis, and research on oral vaccine production, but studies on disaster resistance are still insufficient. (See Non-Patent Document 1).

As a plant cell gene expression machine that ultimately acts on environmental stress, various kinds of genes are involved. In order to discover genes resistant to environmental stress in plants, studies to date have been directed to mutant induction and search, and to compare genes whose expression is amplified or suppressed by differential screening, microarrays, and transcripts using NGS. Although it has been performed by a comparison method, recently, a gain-of-function search method using E. coli, yeast or agrobacteria in addition to a loss of function approach such as a conventional mutation, etc. It has been reported to be effective in the search for environmental stress resistance genes (see Non-Patent Document 2).

However, there have been no studies to search for various environmental stress, especially dry stress resistance genes, in potatoes.

 Mullins et al., 2006  Eswaran et al., 2010  Hemavathi et al 2010  Burge and Karlin 1998  Salamov and Solovyev 2000  Altschul et al 1990

SUMMARY OF THE INVENTION An object of the present invention is to provide a potato-derived dry stress resistant gene and a dry stress resistant potato transformant prepared using the same in order to cultivate a gene having excellent dry resistance and potato varieties using the same.

Hereinafter, terms used in the present invention will be described.

As used herein, the term “dry stress resistance” refers to resistance that prevents the growth of living organisms under dry drought stress.

As used herein, the term “transformer” is genetically engineered to include foreign polynucleotide sequences and refers to those that are designed to be expressed in plant cells, plant tissues or plants to express foreign polynucleotide sequences.

Hereinafter, the present invention will be described in detail.

In order to achieve the above object, the present invention provides an expression vector for producing a cold stress resistant transformant comprising a dry stress resistant gene, the gene provides an expression vector is a gene selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 87. do.

The gene may be composed of all or part of the nucleotide sequence of any one of SEQ ID NO: 1 to SEQ ID NO: 87, 70% or more, preferably 80% or more of any one of SEQ ID NO: 1 to SEQ ID NO: 87 , More preferably at least 90%, most preferably at least 95% homology. The gene may be isolated from a plant, preferably potato ( Solanum tuberosum ).

The expression vector of the present invention may further include a gene, a gene having homology with the gene, or other dry stress resistance gene. The expression vector may be used as long as it is commonly used as an expression vector for plant transformation in the art.

In a preferred embodiment of the present invention, the expression vector may include a promoter and a terminator. The promoter includes a CMV (Cauliflower Mosaic Virus) 35S promoter, a CMV 19S promoter, and a nopaline synthase (nos) promoter of the Agrobacterium tumefaciens Ti plasmid as a promoter for plant expression, ocs (octopine synthase). Both promoters and mas (mannopine synthase) promoters and known promoters can be used.

In another embodiment of the present invention, the expression vector may further comprise a selection marker that can confirm the expression of the gene of the present invention or select a transformant. Selectable markers include genes that are resistant to antibiotics such as kanamycin, hygromycin, gentamicin and bleomycin, or β-glucuronidase (GUS), chloramphenicol acetyltransferase (CAT), luciferase or green fluorescent protein (GFP). Genes encoding such as but not limited to. The expression vector containing the selection marker is delivered to the plant to enable selection of the transformants by culturing in a medium containing a specific antibiotic.

In a preferred embodiment of the present invention, the inventors of the present invention derived from potato plants expressed under a drought environment in order to produce a transgenic plant resistant to dry stress by discovering and identifying the candidate genes adaptive to a dry environment The gene was identified. At this time, the dry stress resistance gene is selected through function-acquisition screening of the cDNA library using the yeast system. That is, the gene cDNA isolated from the potato exposed to dry stress was cloned into the yeast expression vector (see FIG. 3), the yeast transformed with the expression vector was exposed to a dry environment, and then the plasmid was separated from the dry stress resistant yeast. Thereby obtaining a gene having resistance to dry stress (see FIGS. 5 to 8). As a result of sequencing the genes obtained as described above, it was confirmed that the genes shown in Table 1 are expressed in yeast having dry stress resistance (see Table 1).

Therefore, when the gene described in any one of SEQ ID NO: 1 to SEQ ID NO: 87 of the present invention is expressed in potatoes can be usefully used as a gene for imparting resistance to dry stress.

The present invention also provides a dry stress resistant transformant prepared by introducing the expression vector into a host cell.

In a preferred embodiment of the present invention, the transformant is preferably a plant, and includes plant cells, plant tissues or plant seeds. The host cell includes plant cells, plants and culture cells derived therefrom.

The plant may be monocotyledonous or dicotyledonous plants, potatoes, onions, eggplants, tobacco, carrots, cucumbers, olives, sweet potatoes, Chinese cabbage, cabbage, radish, watermelon, melon, cucumber, pumpkin, strawberries, soybeans, green beans, kidney beans, Lettuce, pepper, tomato, broccoli, sunflower, mustard, grass, baby pole, rapeseed, and any one of the plants selected from the group consisting of fresh, of which potato is more preferred, but is not limited thereto.

Transformants of the present invention can be prepared by known techniques, for example Agrobacterium sp.-mediated methods, gene guns, silicon carbide whiskers, The gene of the present invention can be introduced into a plant by a method such as sonication, electroporation, or polyethylene glycol (PEG) precipitation.

In addition, the present invention comprises the steps of preparing the expression vector;

Preparing a transgenic plant cell by introducing the expression vector into a host plant cell; And

It provides a method for producing a plant having a dry stress resistance comprising the step of recultivating the transformed plant cells in tissue culture.

The step of introducing the expression vector into the host plant cell may introduce the expression vector into the plant by a known plant transformation method. That is, to the person skilled in the art or suitable means for transforming the plant, such as Agrobacterium-mediated method, gene gun, silicon carbide whisker method, sonication method, electroporation and PEG precipitation method Any known method can be used.

After transformation, they can be regenerated from plant cells into mature plants, and techniques for regeneration of mature plants from callus or protoplast cultures are already well known in the art for numerous species (Handbook of Plant Cell Culture, Volume 1-5). , 1983-1989 Momillan, NY).

In one embodiment of the present invention, the inventors of the present invention, in order to obtain a gene capable of enhancing dry stress resistance in plants, first applying a drought condition to potatoes, and then produced a cDNA library to express yeast cDNA clones expressed under dry stress Screening is performed using the functional screening method used.

Therefore, a transformant may be prepared by introducing a gene of the present invention into a host cell, and the transformant thus prepared may have dry stress resistance due to expression of the gene.

In addition, the present invention provides a method for increasing the dry stress resistance of a plant comprising the step of introducing the expression vector into the plant. Method for increasing the dry stress resistance of the plant according to the present invention is characterized by using a gene derived from potato that has been confirmed to have a function of increasing the dry stress resistance of the plant, thereby producing a plant exhibiting resistance in a dry environment can do.

According to the present invention, the gene of the present invention isolated from the potato is a gene induced by dry stress, and has an effect of increasing the dry stress resistance of the plant by being expressed in the plant.

1 is a schematic diagram for screening potato-derived dry stress resistant genes.
Figure 2 shows various time intervals (30 minutes, 1 hour, 2 hours, 3 hours, 4 hours, 6 hours, 9 hours, and 24) under dry stress (25%, 27.5% or 30% PEG treatment) for RNA isolation. Time) It is a photograph showing the recovered potato plant.
3 is a schematic of the pYES2.1 / V5-His-TOPO vector used in the present invention.
4 is a photograph showing the viability of yeast according to various drought environments (various sorbitol concentrations).
5 shows screening of yeast transformants into which dry stress resistant genes are introduced in various drought environments.
FIG. 6 is a photograph showing comparison of growth of control transformant colonies and dry stress resistant transformant colonies on a control plate and a dry stress screening plate.
Figure 7 is a photograph showing the growth of the yeast transformant of the present invention in a plate of a dry stress environment containing 2.1 M sorbitol.
8 is a photograph showing a change in gene expression according to the dry stress treatment.

Hereinafter, the present invention will be described in more detail by way of examples.

However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

< Example  1> Preparation of Potato Plants

Potatoes ( Solanum tuberosum ) cultivar "Taston Valley" was produced by Murashige and Skoog (MS) medium (Murashige and Skoog 1962) containing 2.5% (w / v) sucrose and 0.8% (w / v) plant agar (Duchefa, Germany). Growing in a culture tube containing. This potato plant was maintained in a growth chamber of 22 ± 2 ° C. and 50 to 60% RH by irradiating with 10 mmol / m ² s light with a white fluorescent lamp for 16 hours (persons): 8 hours (cancer) with a photoperiod. (Refer nonpatent literature 3).

< Example  2> dry stress treatment

Potato plants grown for 26 days as in Example 1 were transferred to a tube containing 0.5 × MS (Sigma-Aldrich) and 0.5% sucrose solution and incubated for 3 days, and then cultured in an open chamber at 25 ° C. and 60% RH. Different concentrations of PEG (25%, 27.5% or 30%) were treated to give dry stress. Thereafter, 8 to 10 plants were recovered for each sample at a defined time point (30 minutes, 1 hour, 2 hours, 3 hours, 4 hours, 6 hours, 9 hours and 24 hours later) (FIG. 2). The recovered plants were then frozen in liquid nitrogen and stored at -80 ° C until analysis. At this time, the potato plants not treated with PEG were recovered at each of the above time points and used as controls.

< Example  3> cDNA  Production of expression vector

<3-1> total RNA Separation of

To obtain cDNA from the dry stressed potato plants and control samples obtained from Example 2, each total RNA was isolated using the NucleoSpin RNA Plant mini prep Kit (Macherey-Nagel, Germany) according to the manufacturer's instructions. . The purity of total RNA was confirmed through formaldehyde agarose gel and measured using Nanodrop.

<3-2> cDNA Production

The directional cDNA library was prepared from the control RNA isolated from Example <3-1> or from the total plant-derived total RNA under dry stress using an Infusion SMARTer directional cDNA library construction kit (BD Clone Tech, USA). Specifically, the first strand cDNA was a 3 'Infusion SMARTer CDS primer (12μM), SMARTer V oligonucleotide (12μM), 5' PCR primer II A (12μM) and SMARTscribe reverse transcription according to the manufacturer's instructions (Ref of Smart cDNA Kit) Synthesis of poly (A) ⁺ RNA 500 ng using polymerase (100 U / μl). Double-stranded cDNA was synthesized by long length PCR using Accu power HF PCR Pre Mix (Bioneer, Korea), and PCR amplification was carried out by iCycler (Bio-Rad, USA) according to the following program: 1 minute at 95 ℃ Early degeneration; Denature for 15 seconds at 95 ° C. and 18 cycles of annealing / extension at 30 ° C. for 30 seconds, and a final extension of 6 minutes at 68 ° C. Then, cDNA was extracted and separated by size using CHROMA SPIN + TE-1000 column (BD Clone Tech, USA). Yield and purity of the amplification product (amplicon) was confirmed on 1.2% agarose gel.

<3-3> Production of yeast expression vector

E. coli against Amp ^r to express the cDNA library of dry stressed potato plants in yeast An yeast expression vector pYES2.1 TOPO TA (Invitrogen, Carlsbad, USA), an E. coli -yeast shuttle vector comprising a selection marker and a URA3 marker, a yeast selection marker, was used. To prepare the In-Fusion SMARTer cDNA library, the yeast expression vector pYES2.1 TOPO TA was linearized according to the manufacturer's protocol. Linearization of the pYES2.1 TOPO TA yeast vector was carried out using Prime 1 using Accu power HF PCR Pre Mix (Bioneer Corp, Korea); 5′-TTGATACCACTGCTTAGGGCGAGCTTAATATTCCCT-3 ′ (10 μM) (SEQ ID NO: 88) and primer-2; A reaction volume of 20 μl containing 5′-TCTCATCGTACCCCGCTCCTCGGTCTCGATTCTACG-3 ′ (SEQ ID NO: 89) was performed. Amplification was then performed according to the following PCR program: Initial denaturation at 95 ° C. for 5 minutes; 20 cycles of annealing / extension at 95 ° C. for 1 minute and 5.48 minutes at 68 ° C .; And final extension at 72 ° C. for 10 minutes. The amplified PCR product was separated on 1.2% agarose gel and subjected to gel elution of pYES2.1 TOPO TA plasmid (5.8 Kb) according to the protocol of Hiyield Gel / PCR DNA fragments extraction Kit (RBC, Real Genomics, Taiwan). .

<3-4> cDNA  Construction of Expression Libraries

To control gene expression of the library in yeast, cloning of external cDNAs made from potato plants using the Infusion HD cloning kit (BD Clone Tech, USA) downstream of the GAL1 promoter of the linearized yeast vector pYES2.1 TOPO TA (FIG. 3). cDNA libraries of dry stressed potato plants in pYES2.1 TOPO TA were transformed into E. coli HST08 premium Electro-cells (Takara Bio INC, Japan). Then, transformed colonies were screened using PCR to confirm the proportion of recombinant clones as presented in the Infusion SMARTer directional cDNA library construction kit (BD Clone Tech, USA). Two cDNA expression libraries were obtained, consisting of individual clones, controlled under the yeast GLA1 system, respectively, from potato plants subjected to dry stress for 2 and 24 hours. Then, plasmid DNA was isolated from the entire library prepared from potato plants using Gene All Exprep Plasmid Midi prep Kit (Gene All Biotech, Korea).

< Example  4> functional analysis

<4-1> Determination of Functional Screening Drying Conditions for Yeast Strains

Wild type yeast ( Saccharomyces) cerevisiae ) To examine the acceptable or unacceptable drying conditions for strain BY4741 (MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0) (EURO-SCARF, Germany), the yeast was treated with YPD containing 0.1-3 M of sorbitol. Incubate overnight in the medium.

As a result, in the yeast strain cultured in a medium containing 2.1 M of sorbitol, growth defects were observed, so sorbitol 2.1 M was selected as a screening drying condition of yeast (Fig. 4).

<4-2> Transformation of Library Plasmids into Yeast and Treatment of Dry Stress

Yeast (pYES2.1 TOPO TA) plasmids regulated by the yeast GAL1 promoter and containing cloned inserts from potato plants were electroporated using a Biorad Micro Pulser (Biorad, USA) to wild-type yeast strain BY4741. Transformation by law (see L. Benatuil et al 2010). Immediately after electroporation, the yeast was transferred to 8 ml of a mixture of 1 M sorbitol (Sigma Aldrich) and YPD medium in a 1: 1 ratio, and incubated in a shaking incubator at 30 ° C. for 1 hour at 225 rpm. Finally, cells were obtained by centrifugation at 3000 rpm for 3 minutes and the pellet was resuspended in 250 µl of medium. The resulting cell mixture was plated on a synthetic selection medium containing 2% micro agar (Duchefa, Netherlands) to give 1 to 2.1 M of sorbitol, 20 g / L D-galactose and 0.5 g / L raffinose to give dry stress. It was incubated for 3-4 days in uracil-free yeast synthetic dropout medium (Sigma Aldrich).

<4-3> Yeast Functional Screening

After the dry stress was applied to the yeast transformants expressing the cDNA of the potato plants subjected to the dry stress, the surviving yeast transformants were identified, and the control plate without sorbitol and the dry stress screening plate containing sorbitol were found. Yeast transformants of the invention were plated.

As a result, enzyme cells survived in a concentration-dependent manner even with sorbitol by a plasmid having a URA3 marker expressing heterologous potato cDNA (FIG. 5), from which 200 yeast transformants survived under dry stress. It was. In addition, it was confirmed that the yeast transformants grow in the control plate and the plate treated with sorbitol (FIGS. 6 and 7).

<4-4> Plasmid Separation and E. coli Of Inverse transformation

Plasmid DNA was isolated from the dry stress resistant yeast transformants including inserts in the pYES2.1 TOPO TA yeast vector with a slight modification to the method reported in the prior art (Byrd and St-Arnaud, 2001). Specifically, the dry stress resistant yeast transformants were incubated at 30 ° C. in synthetic selection medium containing dextrose for 2-3 days until saturated. The transformants were then washed with 0.3 g of acid washed glass beads, yeast lysis solution (2% (v / v) Triton X-100, 1% (w / v) sodium dodecyl sulfate, 100 mM NaCl, 10 mM). 200 μl of Tris-HCL (pH 8.0), 1 mM EDTA, and 200 μl of chloropan (phenol: chloroform: isoamyl alcohol = 25: 24: 1) were added and vortexed for 5 minutes to dissolve. The plasmid DNA was isolated from the yeast by chloropan extraction followed by ethanol precipitation plasmid of nucleic acid one or more times.

To further analyze the insert cloned in the pYES2.1 TOPO TA yeast vector, the plasmid DNA isolated from the dry stress resistant yeast transformants were transformed back into E. coli via electroporation.

< Example  5> DNA  Sequencing and Annotation ( annotation )

Sequences of cDNAs isolated from dry stress resistant plasmids expressing heterologous cDNAs were analyzed using a Big Dye ^™ Terminator Cycle Sequencing Kit (ABI, USA) and a DNA analyzer (ABI 3730XL, USA). Sequencing reactions were performed using GAL1 forward sequencing primer 5'-AATATACCTCTATACTTTAACGTC-3 '(SEQ ID NO: 90) and prior to further analysis, VecScreen ( http://www.ncbi.nlm.nih.gov/VecScreen/VecScreen.html ). Vector and adapter sequences were cut out using. After pretreatment, GeneScan ( http://genes.mit.edu/GENSCAN.html ) and FGENESH ( http :) for sequences not identified as possible genes via GENSCAN (see Non-Patent Document 4 and Non-Patent Document 5). //linux1.softberry.com/berry.phtml ? topic = fgenesh & group = programs & subgroup = gfind) were used to predict possible open reading frames. In addition, in order to confirm gene function, NCBI ( http://www.ncbi.nlm.nih.gov/blast/ ) and TIGR plantt ranscript assemblies (http://tigrblast.tigr.org/euk) using the BLAST algorithm -blast / plantta-blast.cgi) was subjected to homology search to the sequence database (see Non-Patent Document 6).

As a result, the cDNA sequence separated from the dry stress resistant plasmid was confirmed to match the gene sequence shown in Table 1, it was found that the following genes are genes involved in dry stress resistance in potato plants.

SEQ ID NO: Clone ID
Putative gene function Gene length
(bp) One StDT3 O-linked GlcNAc transferase 903 2 Stdt10 Phospholipase D, partial 444 3 Stdt11  AAA-metalloprotease FtsH, partial 767 4 Stdt15 Steroid dehydrogenase 1437 5 Stdt20 Non-specific lipid-transfer protein 2 345 6 Stdt21 N-acetyltransferase 525 7 Stdt22 Proteinase inhibitor type-2 CEVI57 234 8 Stdt24 SUMO E2 conjugating enzyme SCE1 483 9 StDT25, StDT166 Dihydrolipoyl dehydrogenase, partial 861 10 Stdt26 Ribosomal protein S27 261 11 Stdt28 Cytochrome c 339 12 D29 Metallothionein-like protein, partial 177 13 StDT30,
Stdt93 Anaerobic basic leucine zipper protein 417 14 Stdt31 Dehydration-induced protein ERD15 471 15 StDT33, StDT135 Putative acid phosphatase 855 16 Stdt41 Chitinase 192 17 Stdt43 Glyceraldehyde 3-phosphate dehydrogenase 1014 18 D45 p23 co-chaperone, partial 684 19 Stdt48 Acyl-CoA-binding protein 273 20 D179 Conserved gene of unknown function, partial 975 21 Stdt50 RNA recognition motif containing protein, partial 360 22 Stdt51 Thioredoxin H-type 2 357 23 Stdt52 Protein LE25 273 24 Stdt53 60S ribosomal protein L12 501 25 Stdt54 Putative p23 co-chaperone 771 26 Stdt56 Abscisic acid and environmental stress-inducible protein TAS14 438 27 Stdt57 Stress-associated protein 3 516 28 D58 WD-repeat protein, partial 882 29 D59 30S ribosomal protein S7, partial 30 D62, StDT150 Metallothionein-like protein 249 31 Stdt63 Aspartic protease, partial 1056 32 Stdt64 Endomembrane protein emp70, partial 1041 33 Stdt67 60S ribosomal protein L10-like protein 660 34 Stdt71 Protein transport SEC13-like protein 981 35 D72, StDT52 60S ribosomal protein L12 501 36 Stdt74 Fiber protein Fb11 198 37 Stdt75 Short-chain alcohol dehydrogenase, partial 405 38 D78 Unknown clone, perfect match 225 39 Stdt79 Dehydration-responsive protein RD22, partial 414 40 Stdt80 Ubiquitin-conjugating enzyme 2 483 41 Stdt82 Proteinase inhibitor I 354 42 D85 60S ribosomal protein L27, partial 168 43 D87 Protein transport protein Sec23, partial 552 44 Stdt93 Anaerobic basic leucine zipper protein 417 45 Stdt94 Non-specific lipid transfer protein 2 345 46 D99, StDT111 40s ribosomal protein S11 480 47 Stdt100 Ubiquitin-conjugating enzyme E2 36 462 48 Stdt102 Translation initiation factor eIF-1A, partial 315 49 Stdt103 Aspartic protease inhibitor 5 657 50 Stdt105 Lipid transfer protein 498 51 Stdt110 Photosystem II 10 kDa polypeptide 417 52 Stdt111 40s ribosomal protein S11 480 53 D112 25 kDa protein dehydrin, partial 528 54 Stdt116 60S ribosomal protein L18a 537 55 D118 Conserved gene of unknown function 261 56 Stdt119 Stem 28 kDa glycoprotein, partial 417 57 D121 Glycosyltransferase gene, partial 996 58 D122, StDT22 Proteinase inhibitor type-2 CEVI57 234 59 D126, D25, StDT166 Dihydrolipoyl dehydrogenase, partial 861 60 Stdt134 UDP-glucose: glucosyltransferase, partial 888 61 Stdt135 Putative acid phosphatase 855 62 D136, StDT102 Translation initiation factor eIF-1A, partial 315 63 Stdt138 ADP, ATP carrier protein, partial 951 64 D141 Conserved gene of unknown function 888 65 Stdt147 60S ribosomal protein L1, partial 966 66 Stdt149 Inducible plastid-lipid associated protein 519 67 Stdt150 Metallothionein-like protein 249 68 D151 Tyramine N-feruloyltransferase 4/11 153 69 D152, StDT94 Non-specific lipid transfer protein 2 345 70 Stdt154 ATP-dependent Clp protease proteolytic subunit 906 71 Stdt160 Fructokinase 3, partial 609 72 Stdt162 4F5 protein family protein 204 73 Stdt166 Dihydrolipoyl dehydrogenase, partial 861 74 Stdt171 Hydrophobic protein OSR8 216 75 Stdt174 Putative uncharacterized protein 621 76 D179 Conserved gene of unknown function, partial 975 77 Stdt181 Ribosomal protein L36 333 78 Stdt182 Wound-induced protein WIN2 636 79 Stdt184 Alpha-1,4-glucan-protein synthase [UDP-forming] 1, partial 981 80 Stdt185 Lipase, partial 843 81 Stdt188 Abscisic stress ripening protein (ASR) partial 300 82 Stdt189 Putative uncharacterized protein 1488 83 Stdt190 Stress enhanced protein 2 579 84 D200 Gene of unknown function 411 85 Stdt202 Putative thioredoxin m2 549 86 D205, StDT94 Non-specific lipid transfer protein 2 345 87 Stdt213 Hypothetical protein 612

< Example  6> qRT - PCR through Transcript  analysis

3-4 treated with 2.1 M sorbitol at different time intervals (0, 30 minutes, 2 hours, 6 hours, 12 hours and 24 hours) using the NucleoSpin RNA Plant mini prep Kit (Macherey-Nagel, Germany) Total RNA was extracted from the potato plants of age. For cDNA production, the first strand was synthesized from about 750 ng of total RNA using RocketScript reverse transcriptase (Bioneer, Korea) and oligoDT according to the ccuPower RocketScript RT PreMix Kit (Bioneer, Korea). RT-PCR was performed using Accupower® 2x greenstar qpcr master mix containing 5 μl cDNA template and 1 μl of gene specific primers. The RT-PCR was performed using a Bio-Rad CFX96 sequence detector (Bio-Rad, USA) under the following conditions; 10 minutes at 95 ° C; 29 cycles of 20 seconds at 95 ° C and 45 seconds at 60 ° C. At this time, potato actin gene was used as an internal standard for gene expression analysis. The obtained relative threshold (Ct) was normalized to the actin and the relative expression levels of the different genes from the samples obtained at different time intervals were calculated.

As a result, SUMO E2 conjugating enzyme, Ribosomal protein S27, Cytochrome C, Acyl-CoA-binding protein, 60S ribosomal protein L12, 60S ribosomal protein L10-like protein, Ubiquitin-conjugating enzyme 2, Photosystem II 10 kDa polypeptide and 60S ribosomal protein The expression level of the L18a gene in the potato plant was changed depending on the dry stress treatment time and the treatment time, and particularly, the genes that significantly increased the expression at low temperature stress were identified (FIG. 8).

<110> Konkuk University Industrial Cooperation Corp <120> Drought stress resistant gene from potato and drought resistant          transformant using the same <130> 2012-DPA-265 <160> 90 <170> Kopatentin 2.0 <210> 1 <211> 1179 <212> DNA <213> Solanum tuberosum <400> 1 ggcacaacac ggcgacagca gcagcataag caccactttg aagtaggggg agaattgaat 60 agagggtaag aagaagaaga agatggtaac aagaacagaa gaagaagagg tgaacagatt 120 ggagaatcaa gtagagaatg gagggggagg tgtttgggaa tatctttgtc ttgtccataa 180 actcaagctc cgtcgatctg atatggtgtt gaagcatggg ctctccattc tcaatgattc 240 caaaaagaga tctgctcttg gacctgaaga atggacccta tatgaacagg tcgctgttgc 300 agctatggat tgtcaatcta ttgatgtggc aaaggactgc ataaaggttt tgcaaagaaa 360 gtttccaggg agcaaaagag ttggtaggct agaagctatg ttgcttgagg ccaggggatt 420 atggtcagag gcagaaaatg cttactcaag ccttttggag gaaaatccat ttgatcaggt 480 tgtacataag aggagggtag ccatggcaaa ggcgcaaggc aatacatcag cagcaattga 540 ctggcttaac aagtatcttg acttattcat ggctgatcat gaagcttgga gagagcttgc 600 tgaaatatat gtttccttgc aaatgtacaa gcaagcagct ttctgctatg aggagctcat 660 cttgtctcaa cctacagttc ctttatatca cctagcctac gctgatgtgc tctatacact 720 tggtggacta gaaaatcttc agacggcaaa gaaatattat gcatctacca tagacttgac 780 tggtggcaag aataccagag cactttttgg catatgtttg tgtacgtctg ccatcgggca 840 actgactaaa ggacgaaaca aggaggataa agagagccca gagcttcaat ctctgtcggc 900 aatggcattg gaaaaagact ataagcagag agctcccagc aggctgtcgg tgcttagttc 960 aacattgaga agcttgaaaa tctgactaca cattagcata tagttttatt gagcagcaca 1020 tactgtcaca gcgtgcactc agatcagtct gtcatctgct ataccctttt gcgatttttt 1080 tcccaatagt atcatcttct actcttgcat agtacctatt ttggagtgcc gtttccttat 1140 ttagtagggg aagcaatgac aatgccagtt gtccgagat 1179 <210> 2 <211> 805 <212> DNA <213> Solanum tuberosum <400> 2 atttacgttc atgccaaaat gatgatagtg gatgatgaat acattatcat tggatcagct 60 aatatcaatc aaaggtcaat ggatggtgcc agagattcag aaatagcaat gggagcctac 120 caaccatttc atctctgtgt gaaggagcca gctaggggcc aggttcacgg gttccgtatg 180 gcattgtggt acgagcactt aggcatgtta gacaacagtt tccttcaacc agagagcgta 240 gaatgcatcc gaaaggtgaa caaaattggt gacaaatatt gggatatgta ttcaagtgag 300 agcctcattc atgatttgcc tggacatctt cttacttacc ctattggtat tactgaaaat 360 ggagaggtca ctggaatacc aggagtagaa tgctttcctg acaccaaagc acctgttctt 420 ggtaccaaat ccaattttct tcctcccatt ctcacaacct aagcctccaa cttttaattt 480 gttcattcct tctctagaca tatgttatga ttgtggagta tatgttaata aggtttctta 540 tttaaggaaa gaaaagttta ctatcaccac aggacgcggt ggaatggtta aggtttctct 600 atccttaagc agaggtttca ggttcgatat ttgggaatgg aacctctatc ccttggtatg 660 aattacgaaa ccccagagtg gcgggctttc ttcaagtcgg tgactttgag caccagaaga 720 ttgtacaaaa aaaaaaacag caggctacta ctatatttac catatatagt agtttgtgta 780 gttacgaaaa aaaaaaaaaa aaaaa 805 <210> 3 <211> 957 <212> DNA <213> Solanum tuberosum <400> 3 aaaccagaca taaaaggccg tgatcagata ttcaggatct atttgagcaa gttgaaactt 60 gaccatgagg catcctttta ttcccagagg cttgctgctc taacaccagg atttgctgga 120 gcagacattg caaatgtttg taatgaagct gctttaattg ccgcaaggaa tgagagcaca 180 ataattacaa tgcaacattt tgagtcagcc atagataggg taattggtgg tcttgagaag 240 aagaataagg tcataagcaa gctagagagg cggacagttg cctatcatga atctggtcat 300 gctgttgctg gatggttttt ggaacatgca gaaccattac taaaagtgac aattgttcct 360 cggggtacgg cagccttggg atttgctcag tatgttccta atgaaaatct tctaatgact 420 aaggaacagc tatttgatgt gacatgcatg actcttggag gtcgagctgc tgagcaggtt 480 ttgattggga ggatctcgac tggtgcccaa aatgatttgg agaaagtcac caaaatgaca 540 tatgcccaag tagcagtcta tggtttcagt gacaaggttg gtcttctttc tttcccacaa 600 agagacgatg gatttgagat gtcaaagcct tacagtagca agacagcagc aattatcgac 660 actgaagtaa gagaatgggt ctccaaggca tatgaacgca ctgtgcaact tatagagaag 720 cacaaagaac atgtagcaca gattgcagaa ttactacttg agaaagaggt ccttcatcaa 780 gaagatttgg ttcgggtatt gggtgaacgc ccattcaaga gtctcgagcc cacaaactat 840 gatatattca agcaaggatt cgaagaagag aacaaagaaa gaaaagataa ccccgaaaat 900 aagactgtag aagataacgg atcaccaccg gtcgtacctg aggttgttcc attgtag 957 <210> 4 <211> 1702 <212> DNA <213> Solanum tuberosum <400> 4 ggggaaacga cggagtacta ctctgttgtg gttccaaaag aggaaaaact ttgttagcat 60 taggttgggg gaagaaaaat gcatctgagc gaaaacgaag ggatagagaa gaagagtttc 120 gtggttacag gtggacttgg ctttataggc gccgcacttt gtcttgaact cgtcagaagg 180 ggtgctcgtc ttgtcaaagc atttgatctt agaacccatt ctccttggtc atctcaactc 240 cgtcaatatg gggttcacct cattcaaggg gatataacag acaaacaaca tgtccagaaa 300 gcactacaag ggtcagattg tgttttccat cttgcttctt atggcatgtc ggggaaagaa 360 atgcttcaat atagtcgtgt tgatgaggtc aatattaatg gaacctgcca cattattgat 420 gcttgccttg accatcagat taagaggctt gtttatgtca gcacacctaa tgttgtttat 480 ggtggcaagg aaattgttaa tggcaatgag aacctacctt attttccaat agatgaccat 540 gttgatccat atggacgaag caaatcaatt gctgaacaat tggtcctaaa gagtaacggt 600 cgtcccttca ctaagaagaa tggcaaatgc ctttacacct gcgcaattcg tccagctgct 660 atatatggtc caggtgaaga aaggcacctg ccgaggatca taactcttac aaagctaggt 720 ctgtttccct ttaaaatcgg ttcaccaaat gtaaaatcag attgggtgta cgtcgacaat 780 cttgtactgg cgcttttatt ggctagcatg ggacttttgg atgacatccc tggcagagaa 840 gggcttccaa ttgctgctgg tcaaccttat tttatatcag atggttctcc catcaacagt 900 ttcgagttcc ttctcccttt actgaaaagc ctggattatg atctaccaaa gacatcacta 960 gctgtctctc atgctcttct tctgggaaaa attttctggg ctttctactc ctttctgtat 1020 ccttggttga atagtaggtg gcttcctcaa cctctgatcc ttcctgctga agtctacaag 1080 gttggtgtca ctcattactt ttctttccta aaagcaaaag aggaacttgg gtacgtccca 1140 atggtgagtt ctaaggaggg catggctgca actattgcat attggcaaga aagaaaacgg 1200 aggagtttgg acggacctac aatatgggca tggctgtttt gtgtgattgg aatgtcggca 1260 ttgtttgctg ctgcgtatct gcctgattat ggacccattc cctttattag agcagttcac 1320 ctcttcttct ttcgctctat gttggcattg aaagtgattt ttgttctagc agctgctgca 1380 cacgtaggtg aagctatata tgcatggaat gttgcaaaaa caatagatcc tgcaaatgca 1440 agagggtggt tttggcagac atttgctttg gggatatttt ccctacgatt acttctgaag 1500 agagctaaaa agtaggatta ccatatttct tgctcgactc ttaactatga agcatgtaat 1560 tgcaatcaca tcaaagttga gacataatga caacttttca gtactcaagt tttttctcct 1620 tgtttttggt ctcagcaaac tatctttgtt tttttggtat aataaacgag tttaacttcc 1680 gaaaaaaaaa aaaaaaaaaa aa 1702 <210> 5 <211> 676 <212> DNA <213> Solanum tuberosum <400> 5 cgcagagtac agggacccaa cactcaagta aatacatttg tagtccataa aacttttcta 60 gtatttctat cttttctcaa ttttgtaaca atggaaatgg ttagcaaaat tgcatgcctt 120 gttgttttgt gcatgatagt ggttgcaccc catgcagaag cactaagttg cggccaggtt 180 acatctggct tggctccttg cctcccttat ctccagggac gcggccctct aggagggtgt 240 tgtagtggag ttaagggtct gttgggtgca gccaggaccc cagcggaccg aaagacagca 300 tgtacttgcc tcaaatcagc cgctagtgct attaaaggca ttgatgtggg caaagccgct 360 ggtatcccta gcgtttgtgg cgtaaacatt ccttacaaga taagcccctc cactgactgc 420 tccaaggtcc agtaaggttg atgaaagttc aattttccaa aaggcataat ttggtagtaa 480 tgtgatctag cttgcggaga aaaataaaat ggatatatcg atcgatcgag tttaatccat 540 cattttatat gttgtctctt ttcttttgta tttgtgtttg ttggagtaca tgtaatgaac 600 cttggtggtt ggttgtatta caacccaatc ttcatagtaa tatatacatg atattctcaa 660 aaaaaaaaaa aaaaaa 676 <210> 6 <211> 891 <212> DNA <213> Solanum tuberosum <400> 6 ggggggtcca ttaactggtt atcgccctta cagattacca gaagagttcc gtagagagag 60 aaagagcgaa gaagagagag aaagcatgac gacgattcgt agattcagct gcaatgacct 120 tcttcgcttc gcgtcagtga atttggatca tcttactgaa actttcaaca tgtcatttta 180 tatgacttac atggcaagat ggccggatta tttccatgtt gcaaaagctc ccggcggcca 240 catcatgggt tacattatgg gaaaagttga agggcaaggt gaatcttggc atggtcacgt 300 cactgcagta actgtggctc ctgaatatcg gaggcagcaa ttagccaaga aattgatgaa 360 cctgctggaa gaagtcagtg ataagatcga taaggcttac tttgtggact tgtttgtgag 420 agcatcaaat acaccagcca taaagatgta cgagaagctt gattacataa tatataggcg 480 agttttacgc tattactcgg gggaggaaga tggtttggat atgaggaagg ctctatcgcg 540 agatgtagaa aagaagtcta tcatccctct taaacgacca gtaactccag atgaattgga 600 gtatgactga gtcggtaaga gttgttacca tgtaatctta tctttcaaga agctaagttt 660 aggtatcgag aagtcttgtc tagaaatgta tgagatgaag agtctgggtt cttatcttta 720 gtaatgttat gccaataagt actatgcata ttatgtttaa gaaccaagtg ctagaatttc 780 tggccttgtg tatttgctgc aaccgcagag catttgtatg ttttttaaat tgttaatttt 840 tgtgacttat tgaactttta atatagtttc cgaaaaaaaa aaaaaaaaaa a 891 <210> 7 <211> 529 <212> DNA <213> Solanum tuberosum <400> 7 ttgcctgcct tagcagtggt atcacgcaga gtacagggac atcctccaag aagattagaa 60 aataaaagca aacaatcaag catgggtagt aacaaagttg gtgtcctcac tctcctcctc 120 ctatgtgttt ttctagtggg aagcaattat gtggaagctc agatttgtcc tcaattttgt 180 gagccaaatg tggattacat gacttgttcc tcttcaggaa gcacaatact tcgtccaaca 240 tgcatcaact gtagccaagc aagaggaagg ggatgccaac ttttccgtag agatggctct 300 gcaatatgta actaataaat cacctttgag atttatcaaa ggggatatat taatagtttc 360 taataaatta tgatcatata tatgtgttta aaataaattc tgagtttatg atttactcag 420 atgaccaata ttatatataa ataatggatt tttagtgttt gtctaaaaaa aaaaaaaaaa 480 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 529 <210> 8 <211> 775 <212> DNA <213> Solanum tuberosum <400> 8 gaagacttga gagacaacaa tgtctgcagg tattgctcgt ggccgtcttg ctgaagaacg 60 caaagcttgg cgcaagaatc atccccatgg gtttgtggct aaacctgaga cacttccaga 120 tgggtcagtg aacttgatgg tgtggcactg ctctattcct ggtaaaacag ggacggattg 180 ggaaggtggt tgttatccag ttacgattca cttcagtgaa gattatccta gcaaaccacc 240 aaagtgcaaa ttcccacaag gtttctttca tccgaatgtg tatccatcag gaacagtttg 300 cttgtcgatc ctcaatgaag acagtggttg gagaccagcc attacagtga aacagatact 360 cgttggcatc caagacctgt tagatcagcc aaaccctgct gatcctgctc aaactgaagg 420 gtatcatctc tttattcagg atgctattga gtacaagaag cgggctaagc tgcaggccaa 480 gcaatatccg cctctggtgt agtctaaata atgttcgtat aatcatgtgt atgtgtgtgt 540 tctaatgcaa acttcaaagt aagctgttca tagcgtactg atagctggca ctgttctggc 600 aatatgatgc tgattagttt tgttgcagca gcgaagtaaa tcgttatagt tggaattaat 660 acctgattct gcttttcatt gttcctagtc atcttatatg tgtgtgtatt aacatgcaag 720 ctataattgt ttcagacgat tgaaacatgt cattgataaa aaaaaaaaaa aaaaa 775 <210> 9 <211> 1229 <212> DNA <213> Solanum tuberosum <400> 9 ggtctgaact cagaatttga catttatagt tttaaaaaaa atactatcta ttatcttctc 60 ttccatgttg aatttttgaa ctataaaaac catttgaaaa tgcattcatc atctgtttta 120 tctcctccaa attgctcact actgaagaga tcacacagaa atcaaatcga gaaattgtct 180 ttagcgccat tgaagaatct tccgttctgt ggactgagaa atgagatttt agcgtttgag 240 ttttcgaagc ttaatcgatg tgagactcag agagtgagga attttctaag gaataaaata 300 gtatcagctt cggtggctga aaatggtaat gctccgacgt cgtttgatta tgatttagta 360 ataattggtg ctggtgttgg tggccatggt gctgcattac atgctgtgga gaagggtctt 420 aaaacagcta tcattgaagg agatgtaatc ggcggaactt gtgtaaacag aggttgtgtt 480 ccttctaaag cacttcttgc agttagcggt aggatgcggg aacttaagaa tgagcatcac 540 ttgaaagctt tgggtataca ggtttctggt gccggctatg acaggcaagc agttgctgat 600 catgctaata atcttgcctc gaaaattcgt aataacttga caaactctct aaaggcactt 660 ggtgtggaca ttttgacagg agtgggtact ataacgggtc ctcaaagggt gaagtatgga 720 aaagttggtt tttctggcag tgaagttacc gcaagggata taattatcgc taccggttct 780 gtcccttttg ttcctagagg cattgaagtt gatgggaaga ctgtgattac cagcgaccat 840 gctctcaaac tggagtttgt acctgaatgg atagctatag tagaagtggt tatattggac 900 ttgaatttag tgatgtgtat accgcacttg gtagtgaggt tacttttatt gaagctctag 960 atcacttatg cctggatttg atccagaaga attagtaagt tagctcaaag attctggtaa 1020 tccaggaaaa ttgattatca cacactggat gtttgccacg aagatcactc ccgcaagaga 1080 tggggagaac agtgtttatg actacctgac gcccgacata agagttaaga acctttggag 1140 gtgatgcacc tcatggcact cgagctcaag ttctatgctg gaattacctg tgacacacag 1200 gtatttctgt cgtgagcgac tcgcacagt 1229 <210> 10 <211> 622 <212> DNA <213> Solanum tuberosum <400> 10 gtcttcattt cagctgcctc cgacgctctt cctcccccgc cgccgtccgg aatttcccgt 60 tagcctcgcg aaaatggttc ttccaaacga tgtcgacttg ttgaacccac cagctgagca 120 ggagaagaga aagcacaagc tcaagcgttt ggttcaatct cctaactctt tcttcatgga 180 tgtgaagtgc cagggttgct ttaacataac aacggtgttc agccactcgc agactgtggt 240 ggtgtgcggg aactgccaga cggtgttgtg ccagccaact ggtggacgtg ctagactcac 300 tgagggttgt tctttcagga gaaagggaga ttagagaaga acaggcaact atttgctttt 360 agagtgggaa gtgaaaagtt tatatgggta acaaagtcag ttttggtgtc tcctgttttt 420 tttttcttct tttttgatct tttaagaaca aaggattttt aggttaccat gtctggttat 480 gctatgtttt tcaattaaat tgggtttcaa ttcacttatg gattttgaat tttgctatat 540 attagcgctc tatgttgttc tacctatgat attccccatc aatttctttg aattccttga 600 taaaaaaaaa aaaaaaaaaa aa 622 <210> 11 <211> 679 <212> DNA <213> Solanum tuberosum <400> 11 ggaagaagaa tttccctatt ttatttctca cggccaaacc ccccctctct ctctcgtctg 60 cgtgtgtaga aagtcggaga aatggcatct ttcggggaag caccaccagg taatccaaaa 120 gccggagaaa agatcttcaa gaccaagtgc gctcaatgtc acacagtcga taaaggagct 180 ggtcacaaac aaggacccaa cttgaatggg ctctttggaa gacaatctgg tacaactgca 240 ggttactcat actccaatgc aaacaaaaac atggctgtga cctggggaga gaatacatta 300 tatgactact tgctcaaccc taagaagtat attcctggga caaaaatggt tttccctggg 360 ttgaagaagc cacaggagcg ggcagatctc attgcttatc tcaaggaagc aactgcgtga 420 agatcttttt ggaacattat aatacttttg ctagtggtca ggagtcgtcc attggtagca 480 tttctcattt tatattttga aaggcatatt ttgcccgttt aaataaaacc aaatacagtg 540 tcttgccatt cttctggttg agctaagcca gtcttgaagg aattttgtcc aagagtttgt 600 catttattgt cacataaaat cttgacctac ttatgaatat gcctgattta tttctttcgt 660 aaaaaaaaaa aaaaaaaaa 679 <210> 12 <211> 177 <212> DNA <213> Solanum tuberosum <400> 12 atgtacccag acatgagcta caccgaaagc atcacaacca ccgagacttt ggtgcttggg 60 gtgggacctg agaagacaag cttcggcgcc atggagatgg gtgaatcccc tgttgctgag 120 aatggctgca aatgtggatc tgactgcaag tgcaaccctt gcacttgttc taagtga 177 <210> 13 <211> 689 <212> DNA <213> Solanum tuberosum <400> 13 atgttacctt taaggcagag tgctagttca tctgcatcag atgatgatca aaggtatgca 60 ggaatggatg agaagaagag gaaaaggatg atttccaaca gggaatctgc gaggcgatcg 120 aggatgaaga agcagaagct tctgcaagat ttgacagggg aagtgagcaa attacagtct 180 gcaaataaga atattgtgtc taagattgac gagacaacag aaagatatgc aatttgtgct 240 gcacagaata atgtgttgag ggcacaggca atggaattga ctgataggct caggtattta 300 aatgatgtta ttgacagtac tggtttggct gctggtgctg ctgatccttt gttgaagcca 360 ttgcagaatc cttgtccaat gcagcctatt gcttcttcag gattgtttaa attttaatcg 420 tcgttgtggc ttgtttggat agctttgctt catagtccat attttagtag agtttccttg 480 gatggtatgt catgtagtat gcatttgctg gttttgttta gtgtatgctg tgtctgctac 540 tcttttacca tgtattcaga tcagcttgga gttgtgtgaa atcgatgttt gtttctacga 600 gaataccaat gggttcctat cgtacccgct ctgccgaatt ccacccgtac ggtcatcatt 660 acaatacatg gtagatgttt ctcgtccag 689 <210> 14 <211> 779 <212> DNA <213> Solanum tuberosum <400> 14 gatacgtgta cgagatcgtc tctgtagctg aagaacaaaa aatctgttaa aaggcatcaa 60 ttttgaaaaa gcaatatggc actagtttct ggaggaagat caacattgaa tccaaatgcg 120 cctctctttg ttccttcatt cgtgcgccag gtagaggact tttcaccgga atggtggaat 180 ttggtgacaa cttcaacatg gttccatgac tattggatga gccagaacca gggagaggaa 240 tatggtgctg gtaatgatgt tgctgatttg cttcctgaaa atattgatct caatgttgat 300 gaggacatct tgaacatgga agctcagttt gaggaatttc ttcaatcttc tgaaaatggg 360 caacaaggaa ttaagtcatc tctctatggt atcaatgcga tgccacaata tggttttcca 420 tctgatgcac tgataagaac attgagttca ccaagatccc ccattgggcc acccaaatac 480 tttgagaagg catccaagat cgtgagcccg aggaacagtt tccgcagcat ccagcagcct 540 cgttgaattc ccagtctctt ggtttacttt cagaaagtcg gttgttagtt tgcagcagtt 600 tagaaagtct tttaagcttt agtttcatgt aacctttgta tagagcaagg taggtggcac 660 actcttgtat ctagccttgt tcatatcctt tctttagctt aacttcttgt aaacaacagc 720 actgtaaaag tgaaaaaaat ttgtaaaaac cagttaaact aaaaaaaaaa aaaaaaaaa 779 <210> 15 <211> 996 <212> DNA <213> Solanum tuberosum <400> 15 gacaacatct tctttaattt cttctcttcc atcaaaatga ggtcatgttt cactattctc 60 ttgttatttt cagctatagt agctgtggca ttggcctcta atgttgaaga ggtgataagc 120 caagtagtag aaattcaccg tctaaggcca caaactggtt ctgctggtta taccgtccca 180 catttggact gtcttagctg gcgactcgcc gtggagacaa acaacctcca atactggaaa 240 ttggtgccta aagaatgtac aaactatgta ggtcattaca tgttaggcaa acaatatcgc 300 cacgactgtg aatatgtagc caagcaggct attgagtatg ccaaaagcct caaacttggt 360 ggagatggaa tggacgtttg ggtatttgat attgatgaga ccactctctc aaatcttcct 420 tactacgctc ggtctgacgt tgcttttggg gctatagcgt acaactctac aaagtttcat 480 gagtggatcg cggagggaaa agcgccagcc attccatcgg tactgggtct atacaagatt 540 gtgttgtctt tggggatcaa gcccgttttc attacaggga cgagagaaaa tttcgaacaa 600 gtgaggattg ctaatctcaa gaaagttggt tataccaact gggcagcgct catactaaag 660 ggagaaaatt attcagggtc agcagtgaaa tttaaatcga gcaagagaac agcattggtg 720 aaggctggat atagaattgt tggtaatatt ggagatcaat ggactgattt aatcggagaa 780 aacgttggag ctcgtacttt taaactccct gaccctatgt actacgtagt tgactctaac 840 caaattttaa tcaacaaagg tggtggggta gctttttatt ttggcatgtg agatattata 900 agctcagtac tgttcctaat cccccatcac tttgctataa ataatgactt ccttcatatt 960 ttcacgaaga aacaagagaa aaaaaaaaaa aaaaaa 996 <210> 16 <211> 517 <212> DNA <213> Solanum tuberosum <400> 16 gatatccata actaatatta ctaaatatct caagaaaata agaaaaatga aaatgatgca 60 gtttgttgtg gttattgtga atattttagc attgttgata agtgtagcaa atgcacaaca 120 atgtggaagt caagctggtg gagctttgtg tgccaatggg ttgtgttgta gtgaatatgg 180 ttattgtggc actactactg cttactgtgg atcaggatgc cagagtcaat gcaactaaat 240 tataaggcta tgataagaat aaggtaatat ccatgtggtt gtgtgtttaa aaataatata 300 aatttgtact actagaataa agtgggaaca tgtatgtatt gtgagttcca caagaacaag 360 aatatagtgt caggtcatga acactatata tgtactagtt tgtttgtttt ctatttcaat 420 gtgatgttta ttaaggttgg ttaaaaacca cttaattgaa tgtccaataa gtattattag 480 atcgatccca ataattccaa aaaaaaaaaa aaaaaaa 517 <210> 17 <211> 1014 <212> DNA <213> Solanum tuberosum <400> 17 atggctaagg ttaagattgg aattaacgga tttggaagaa ttggccgatt ggtcgctcgg 60 gttgctctcc aaagagatga tgttgaactt gtcgctgtta acgacccctt catctctgtt 120 gattacatga catatatgtt taagtatgat agtgtacacg gccagtggaa gcatcatgag 180 cttaaggtta aggatgagaa aacccttctc tttggtgaga aggctgttac tgtttttggc 240 tttaggaacc cagaggagat tccatgggca cagactggag ctgattacat tgtggagtcc 300 acgggtgttt tcaccgataa ggacaaagct gctgctcatt tgaagggtgg tgccaagaaa 360 gttatcattt ctgctcctag caaggatgct cctatgtttg tcgttggtgt caatgagaag 420 gaatacaagc cagagctcaa cattgtttca aacgctagct gtaccacaaa ttgccttgct 480 cccttggcca aggttataaa tgacagattt ggaattgttg agggtctcat gaccacagtc 540 cactccatca cagccactca gaagactgtt gatggaccat cagccaagga ttggagaggt 600 ggaagagctg cttcgttcaa cattattccc agcagtactg gagcggccaa ggctgttggg 660 aaagtgctac cagcattgaa tggaaagtta actggaatgg ctttccgagt cccaactgtt 720 gatgtgtctg tggttgacct cacagtgagg ttggaaaaag aagctaccta tgatgaaatc 780 aaggctgcca tcaaggagga gtctgaagga aaactgaagg gaattctagg ttacactgaa 840 gatgatgtgg tttccacgga ctttgtggga gacaatagat caagcatatt tgatgccaag 900 gctggaattg ctttgagcaa gaattttgtc aagcttgttg catggtacga caatgaatgg 960 ggttacagca caagagtggt ggacttgatt attcacatgt catcagttca gtaa 1014 <210> 18 <211> 684 <212> DNA <213> Solanum tuberosum <400> 18 atgagcctcc atccagaagt taagtgggca caaaggcctg atgtggtgta tctcactgtg 60 atgctaccag atgctaaaga tgcaaaagta aacttggatc cagaaggagt gtttaatttc 120 tctgctagtg ctggggcagg agaccgtcac tatgagttga agcttgagct tcaggataaa 180 gttaatgttg aggagagcaa aattagtact ggcgtgagaa gtattttctg cattttgcaa 240 aaagcagagc caaaatggtg gactaaatta ttgcgtggag acggtaaagc accacactat 300 gtgaaggtag attgggacaa gtgggtggat gaagatgatg aacctgatgc tggtcctggg 360 gatatggata tgaattcaat ggatttctct aaattcggtg atatgggtgg aatgggaggt 420 gatatggctt caatgatggg cggaatgggc ggaatgggcg gaatgggtgg aatgatgggc 480 ggaatgggtg ggatgatggg aggaatgggt ggcatgatgg gtggaatggg aggtgatatg 540 gctggaatga tgggtggaat gggaggtgat atgggggatg accttgatga gagtgacgat 600 gaagagcaag aagtgataaa gccttccgct aaagctgctg aggtggtaga tgcgaaacct 660 aaggcagaag gtgaagctgc atag 684 <210> 19 <211> 466 <212> DNA <213> Solanum tuberosum <400> 19 gaagcagatc agatttccat catggctttg aaggaggaat ttgaagcgca cgctgagaaa 60 gctaagacat tgcccgagag taccaccaat gagaacaagc ttattcttta cgggctttac 120 aagcaagcca ccgttggcaa tgtcgacaca agccgtcctg gcatattcaa catgagagac 180 agagcaaagt gggatgcatg gaaggctgtt gaaggaaaat ccaccgatga agccatgaac 240 gactacatca ccaaggtgaa acagttgctg gaagaaggtg ctgcttcagc ataaggctca 300 tctatctatg ttgttaaatt gttattggaa tggactatgt ggcttgtctg attttagtgt 360 aacagcaggt gtatgctttt cctggctagg aaaaaaaact acttctataa gtatgtttga 420 accttctatc aataaacccc ttaaaataaa aaaaaaaaaa aaaaaa 466 <210> 20 <211> 975 <212> DNA <213> Solanum tuberosum <400> 20 atgggtggcg gtggagcatt cagagcagcg gcgaaggtcg ccggagccgc cgctgccaac 60 agcggattcc gctcggtgac ggtggagcat ccagtctatg cagccgcacg caacgtcccc 120 cgtccggtgt cgatctcagg tatatcgtca acatcggaag atgtcaagtc tgctatggtt 180 acggcttctc acggtggatc atcggatgtg tcgtatgtgc ctaagatggt ttctgatttt 240 gatgactggg agatggccgg cggagaggaa gaaatgatgg ttaatcctgg tgaaccgctg 300 ccacgactcg tatttggtgg agctccttct cttcaggaag ctacagaggc tacttctgat 360 ctcaaagatg ctctggtata tatgtctgga tctgctaatg gatatggggg atcttgtata 420 tccggctcat gctcatcgcc cgtctccaaa gcctgtgttg tcagcgagac tatagtcaca 480 aagtctgtgc cgaaacatgc tgttcaggca tataggattc tgagtgaaat tcctgctgct 540 cagaacgttg ttgcttcaat tgcttgtgac ccaaatgtgt ggaatgcagt actgcaaaat 600 ccagcccttc aagatttcct cgagtcacag agatacagtg agaagtgtgc atcattcccc 660 gactcggatc aagagaaaga tgaatcggtt gctgatactg cttccttttc tgaatcatcc 720 ccatggaagc cgttttctga atctaaggcg gaagagtcta aatccgggaa ctcttttaca 780 agctttttgc agaatgttac acaaacagtg acacaaacag tggttagtat gatggacagc 840 ttgtcagatt tctttaacaa tctctttgga ggcaacaagt tttttgttga tgctgatgga 900 agtgctaaat ttggcgcggg tgggagaaag acaccttgga gcatcttttc atggctttgg 960 cagtcatgtt attag 975 <210> 21 <211> 993 <212> DNA <213> Solanum tuberosum <400> 21 cgcagagtac agggtgaatc cagggggtta tacagcagaa gttacaggtc tttcccctgc 60 tgctactgag aaggatgttc aggagttctt tggtttctgt ggggcaattg aacatgttga 120 gattgtcaga gctggtgaac atgcaagtac agcctatgtt acgtttagaa atcctcatgc 180 attggaaacg gctgtcttac tgagtggagc tgccattttg gatcaacgtg tgtgcataac 240 cagctggggt cactatcaag atgaatttga ttattggaat cattcatcat ggaaacctca 300 agaagactgt cattcaagtc aggattccca aggacactat tttgtttctt ctgctgggga 360 agctgtaaca ttgactcaag atgtggttaa aaccatgcta tctcaagggt atgttcttgg 420 aaaaggcgca ttggaaaaag ccaaagcttt tgacgaatct catgggctct cagcaactgc 480 agtctccaag gttgctgatt taagtgagag aatcggcctc actgacaagt tctgtgccgg 540 ggttgaagtg gctagatccg tggatcagag gtatcacata tcagatacca ccagatcagc 600 tgtttcagct acaggtagaa ctgccatctc tgctgcaact gctgttgtca acagtagcta 660 cttttccaaa ggagctcttt ggatgtctgg tgcactaagt aaagcggctc aagctgcagc 720 tgatttggta gccgaggcat taacaaatga gtaggttcga gcaattgaca tgtagcttgg 780 agtcctttat atgagaatgg tattagttct atgggttgtg tggtgtaaag tcgcgaacta 840 agtagattgg cctaatggtg ttgattcatc tggaatcgaa ctgtgtttcc tttccgttgt 900 tgtttggata tcagtcgatg ttgtaaagaa catatagtac gctaatacgt tgagtaggat 960 ttccatttca gctcaaaaaa aaaaaaaaaa aaa 993 <210> 22 <211> 553 <212> DNA <213> Solanum tuberosum <400> 22 agagctttct tgtttggtaa gctaattttt gcgaagccca tagaaaaaag aacaactcag 60 agaaatggca gaggagggac aagtctttgg cgtccacgcc gttgatgaat ggaaccaaca 120 tctccaaaaa ggaattgata acaaaaaact gatagttgtg gactttactg cttcctggtg 180 tggtccctgc aagttcattg ctccattcct tgcagagttg gccaagaaga tacccactgt 240 taccttcctg aaggtggatg tggatgaatt gaagtctgta gctactgatt gggccgtgga 300 ggcgatgcca accttcatgt tcatcaagga ggggaagatc gttgataagg tggtgggagc 360 caaaaaagat gagctgcagc agaccattgc caagcacatc agtagtactt catcagccta 420 attaatatgt tttactccat attatattgc acttggttga gtggcatcta tcttaatatg 480 agcaagaatt aatttaattt tcgttggata ttgcaagttc ttactattaa gccaaaaaaa 540 aaaaaaaaaa aaa 553 <210> 23 <211> 515 <212> DNA <213> Solanum tuberosum <400> 23 gaatcgaaat tacaaacaac aaacacactg tagaaagaag aagagagaaa acgaattaat 60 cgattagcga aaaaaaaaga aaaatgcaga caggaaagga cgcagcatct gcagccaaag 120 ctggcatgga gaagactaaa gcggatcttc aagagaaggg tgagaagatg acgacgaggg 180 atccattgaa gaaagagatg gcgacggaga agaaagagga tagggttgca gcggcggaga 240 tggagaagag agatgctaaa gcacagcatg cagctgcaaa acaaggagga gctacaactg 300 gaactggaac tggaactggt tatggtgcca ccactggcga tcataccact ttttagaaag 360 aaaaaagcta gttactttta attttggtgt ttttttttgt tttgttatta ggggagggat 420 gtgattcaca ctttttgtgt gggtgttggc ttttaagatt gacaccaaat taaataaata 480 aagtggtact cttcctaaaa aaaaaaaaaa aaaaa 515 <210> 24 <211> 709 <212> DNA <213> Solanum tuberosum <400> 24 gctccgaaac acagctaaac ctcaacccta gctcactgtc aaaatgccgc caaagttcga 60 tccatctcag gtcgtggagg ttttcgtccg agtcaccgga ggtgaagtcg gagcagcgag 120 ttcactcgct cccaaaatcg gtccactcgg tctttctcct aaaaaaatcg gagaagacat 180 cgcaaaggag acctcaaagg actggaaggg tctccgagtg accgtcaaac tcacagtgca 240 gaatcgtcaa gctaaggtat cagttgttcc ctccgccgcc gcacttgtca tcaaggcgtt 300 gaaggagccg gaacgtgaca ggaagaagac gaagaacatc aagcataacg gtaacatctc 360 actggatgac gtcattgaga ttgctaaggt gatgcagtct agatctatgg cgaaggattt 420 gagtggaact gtgaaggaga tcttgggaac atgtgtatct gttggttgta cggtagatgg 480 aaaggatcct aaggatttgc agcaagagat tactgatgga gatgtggaga ttcctcagga 540 ttgatgagat aagagggaat tatgaactga tgattccatt gatggaggtt atgttctgat 600 tttgttctta tattaatttt tatgttattt tgctttgagt atgttatcga ggataatttt 660 gatataatta tgacgagtaa actttgggta aaaaaaaaaa aaaaaaaaa 709 <210> 25 <211> 1000 <212> DNA <213> Solanum tuberosum <400> 25 gctacactgt ttttagggtt tttcagtctc tcttccttta acctcttctc atctgctcta 60 gaaaaaaaaa tatattcaaa tatgagcctc catccagaag ttaagtgggc acaaaggcct 120 gatgtggtgt atctcactgt gatgctacca gatgctaaag atgcaaaagt aaacttggat 180 ccagaaggag tgtttaattt ctctgctagt gctggggcag gagaccgtca ctatgagttg 240 aagcttgagc ttcaggataa agttaatgtt gaggagagca aaattagtac tggcgtgaga 300 agtattttct gcattttgca aaaagcagag ccaaaatggt ggactaaatt attgcgtgga 360 gacggtaaag caccacacta tgtgaaggta gattgggaca agtgggtgga tgaagatgat 420 gaacctgatg ctggtcctgg ggatatggat atgaattcaa tggatttctc taaattcggt 480 gatatgggtg gaatgggagg tgatatggct tcaatgatgg gcggaatggg cggaatgggc 540 ggaatgggtg gaatgatggg cggaatgggt gggatgatgg gaggaatggg tggcatgatg 600 ggtggaatgg gaggtgatat ggctggaatg atgggtggaa tgggaggtga tatgggggat 660 gaccttgatg agagtgacga tgaagagcaa gaagtgatca cgccttctgc tacagctgct 720 gacgcgatac ccgcgcactt cacacccccc ctcccgaatg cactgcacga acatgggccc 780 cgaacggaga ccactggggg gagacttcaa ccttcctctt ctccctacac ccgcagactc 840 ttaagtctct gaggagcata aattcgcctc tatgtacagc catacgtgct tcttccagcg 900 gggaccagct taagcaggac tgagacagtc ccaaggtcct ttccaaccta tcgaactgcc 960 caacccctct aaaatcgttg catcctccgc tcctcgatga 1000 <210> 26 <211> 438 <212> DNA <213> Solanum tuberosum <400> 26 atggcacaat acggcaacca agaccaaatg cgcaagacta atgaatatgg aaaccatgtc 60 caagaaactg gaactcatca aggtaccggt atgggaggca tgcaggctgg tactggtggt 120 atgatggggg gcatgggtac tggcggtatg ggtggcactg atggaggata tggaacacgt 180 accggtatgg gaatgggtat gggtactggt acccatcacc atgaggggca acagcagctt 240 cgtcgatccg acagctctag ctcgtcggag gatgatggag aaggtgggag gagaaagaag 300 gggatgaagg agaagataat ggagaagatg ccaggaggag gacatggcca gcaggaagga 360 caaacaacag gtgaaggagc tggagagaag aaaggaatga tggacaaaat caaggacaag 420 atccctggga tgcattga 438 <210> 27 <211> 938 <212> DNA <213> Solanum tuberosum <400> 27 aaacacaatc tctctctctc tcatcggcga tctaaactct atctggggcg gagctaggta 60 ggggtgaggg gttcatccgt aacccctttc ggcaaaagat cacactgtgt gttgacaata 120 tggagcatga tgagacggga tgccaacccc ctccggaagg tcctattttg tgtattaaca 180 actgtggctt tttcggaagt gctgcaaaca tgaacatgtg ctccaagtgt tacaaagaca 240 tggtattgaa acaagaacaa gctaagcttg ctgtgtcatc aattgaaaac cttgtcaatg 300 gatcatctgc cggagagaag ggaatggtta ttgttgaccc cgtagatgtg caacctgata 360 ctattgaagc acagtctata gctttgccat catctcaaac ttcgagttct agtgatatgc 420 ctgatgtaaa ggctaaggtg ggtcctaacc gctgtggcac ttgtaagaaa aaggttggca 480 tcactggatt caaatgtcgc tgtggtaacc tttattgtgg agcacaccgc tactcggata 540 aacatgactg cctgtttgat taccgctctg ctggccagga tgcaatagca aaagccaacc 600 ctgttgttaa ggcagagaag cttgacaaga tctagaaggg gagtatttgg tgatttgatt 660 ttgtctccaa aagggtcctt ctctgattgg caacatatat tttgagatct tattaatcct 720 gggggacgaa gaagaaaaaa tgatatgtgc atgttcaaga attattagga gaatatgtgt 780 gtttgctcca gtttcaggtc tttcatttta ctggtttgat agcactttga ttatgtctgt 840 catatgtcgt tctcactcac ctggtattct atcagatatt acatgtctaa atggtatgtg 900 caaatctgga taacatgcca aaaaaaaaaa aaaaaaaa 938 <210> 28 <211> 882 <212> DNA <213> Solanum tuberosum <400> 28 atgcattggt catcaatttc ccgcaacctg tcagaagttc tcaacttctc tggacgtata 60 gtaccgacag agaaatatgc aggaaatttg ttagagggtc tgacactgac ccaaatcggc 120 acgatggcgg tgaataaccg ttttgtggtt gctggaggct tccaaggaga actcatttgt 180 aagagtttgg acaagccagg ggttagcttc tgtgcaagga ccacttatga ggataatgct 240 atcacaaatg ccgttgagat atatgaaagt gtcagttgtg ggcttcgttt tatggcagcc 300 aacaacgact gtggtgtgag ggtgtatgac atggagaagt ttcagcagat gaaccacttc 360 cgcttccctt ggccagtgaa tcacacctcg atgagcccag attgcaagct ttttactgtt 420 gttggggatg atctagatgg tttacttgtt gattcccgca atggaaagac ggtggcttca 480 attgttggtc atttagacta ctcttttgct tgtgcgtggc atccagatgg gcgtacattt 540 gccactggga atcaagataa gacatgtcga atttgggact tgagaaactt atcctcgtct 600 acagccattc tgaagggaaa catcggtgct gctcgatcta tccgtttctc atccgatgga 660 cagttccttg tagtggctga gcctgcagat tttgtccata tctacagtac caagtcagac 720 tacaagaaac gtcaggaaat tgatcttttc ggtgagatat caggggtatc tctgagtcca 780 gacgacgaat ctctttacat tggtatctgg gatcgaacat atggaagctt gctagaatac 840 aacagaaagc atacttgtag atatcttgat tcgttcgtgt aa 882 <210> 29 <211> 123 <212> DNA <213> Solanum tuberosum <400> 29 atggctttca aattaagttc cgaattagtg gatgctgcca aagggagtgg cgatgccata 60 cgcaaaaagg aagagactca tagaatggca gaggcaaata gagcttttgc acattttcgt 120 taa 123 <210> 30 <211> 559 <212> DNA <213> Solanum tuberosum <400> 30 gactcatttt acatcaatca taacacattt gatttctcaa ttttttaatc tcagatcaat 60 catgtcttgc tgtggaggaa actgtggctg tggatctggc tgcaagtgcg gcaatggctg 120 tggaggatgc aagatgtacc cagacatgag ctacaccgaa agcatcacaa ccaccgagac 180 tttggtgctt ggggtgggac ctgagaagac aagcttcggc gccatggaga tgggtgaatc 240 ccctgttgct gagaatggct gcaaatgtgg atctgactgc aagtgcaacc cttgcacttg 300 ttctaagtga acaaactata gttaaaacag agcagagatc atggtgttag ccttctatgg 360 ctgaagaaaa tagttgaaaa atgtgtgtct ctctagtgtt ttagtgtcag tctgttgaaa 420 cagggaaaaa atatgtgttc tttttttccc tgtaataaga aatatagtaa tggaatgtat 480 aagtaaccag taaaatggtt tgtgtaacat gagcatctct gcatctggta attttaacta 540 aaaaaaaaaa aaaaaaaaa 559 <210> 31 <211> 1277 <212> DNA <213> Solanum tuberosum <400> 31 atacaagaga tggggaatct tgttcaatcc gttatggaac tggatcaatc tctggccatt 60 tcagtatgga caatgttcaa gttggtgatc ttgtagttaa agatcaggtg tttattgagg 120 cgacacgaga acctagtatt acatttatag ttgcaaagtt tgatggtata ctaggccttg 180 gtttccagga aatttctgtt ggaaatacta cacctgtctg gtacaatatg gtggggcaag 240 gtcttgttaa agagtcagtc ttctctttct ggtttaaccg tgatgcaaat gcaaaagagg 300 gaggtgaact tgtttttggt ggagtcgatc caaagcactt caagggtaat catacttatg 360 ttccattgac tcagaagggc tactggcagt ttaatatggg agatttcctt atcgggaaca 420 catcaacagg ttattgtgca ggtggctgtg ctgctattgt ggattctgga acatcactac 480 ttgctggtcc aacaactatc gtgacacaaa tcaaccatgc cattggagca gaaggaatag 540 ttagcatgga atgtaaaact attgtttcac agtatggaga gatgatctgg gacttactag 600 tatcaggggt aagacctgat caagtttgtt cacaagcagg tttatgcttt gttgatggag 660 ctcagcatgt gagctctaat atcagaactg tagttgaaag ggaaactgaa ggaagttctg 720 ttggtgaagc accattatgc actgcctgtg agatggcagt tgtttggatg caaaaccagc 780 tcaaacaagc agggacaaaa gagaaagtgt tagagtatgt gaatcagctt tgtgagaaaa 840 taccaagtcc aatgggagaa tccacaatcg actgtaacag catctcatcc atgccagata 900 tttcattcac tatcaaagat aaagcttttg tgctcactcc agaacagtat attcttaaaa 960 ctggagaagg ggttgcgaca atttgtgtca gtggatttgc tgccctggat gtgccaccac 1020 ctcgcggtcc tctttggatt cttggagatg tattcatggg gccgtatcat actgtgtttg 1080 actatggtaa atcccaagtg ggttttgctg aagctgcata atagcctttc atgtagttac 1140 acgcctcagt tactttgtaa aatggggcaa gtattgtaaa gagatgaatg ctactgctac 1200 tgctatatga ttgtgttgta tctgactata atatggcaca ctagtctctt tcttgaccaa 1260 aaaaaaaaaa aaaaaaa 1277 <210> 32 <211> 1421 <212> DNA <213> Solanum tuberosum <400> 32 atgcggacgc taaggaatga ttatgccaaa tatgctcggg aagatgatga cctggagact 60 ctggaaagag atgtgagtga agagtctggt tggaaacttg ttcatggaga tgtgtttcgg 120 actcccctta acctagtgtt actttctgct cttgttggta ctggggcaca actggcattg 180 cttgtcctcc ttgtcatact gttggcaatt gtggggatgt tgtatgttgg gagaggatcc 240 atagttacga cttttattgt atgttatgct ttgacatcat tcatttcggg ctatgttagt 300 ggtgcaatgt actcacggaa tggtgggaaa agctggatca agtcgatgat tctgacagca 360 tcactctttc catttatgtg ctttggaatt ggcttcgttc tgaacacaat tgccatattt 420 tatgggtctc tagcagcaat tccctttggc acaatggtgg ttgtcttcgt catctgggca 480 ttcatttcct tccccttggc tcttctgggt acagttgttg gaagaaactg gagtggtgct 540 ccaaacaatc cgtgccgtgt caagaccatt cctcgcccta tacctgtgaa aaagtggtac 600 ttgacaccat ctgtaatctc cttgatgggt ggtctgctac catttggcag catatttatc 660 gagatgtatt tcgtcttcac atccttctgg aattacaagg tgtactatgt ttacgggttc 720 atgcttttgg tgttcctgat actcattgtt gttactgtct gtgtgaccat cgtgggcaca 780 tatttcctgc taaatgctga gaattatcat tggcagtgga cttcattctt ctcagcattc 840 tctacagctg tctatgtcta cctgtactca gtatattact actatgtgaa aactaagatg 900 tcagggttct tccagaccag cttctatttt ggatatacac tgatgttttg tcttggctta 960 ggaattctct gcggtgctgt tggatttttg ggctcacatc tgttcgtgag gaggatctat 1020 aggaacatca aatgtgactg atgttttaac aaagaagagt aggactgaca ctgctatatt 1080 tgcttggtat ctagcagtaa ggtcaagaac aacctcaaga accatgtatc tttgtgggat 1140 tcaatttggt ttaatgatgg taggtacctc tgcttgtatt ctggggagcc ctgaaaattt 1200 tccatattcc ttgggctgta aaagaaatct cctattttca tttgtactta aattagaatg 1260 gagactttgg atgattcaag gctagcatgg taagagttac agctgtgatg tagttatatc 1320 tccaatacta tacgacgcag atgcaaaaaa cattgctact taatttttgt ttattcttta 1380 Tgcccagaca agttgttgaa caaaaaaaaa aaaaaaaaaa a 1421 <210> 33 <211> 876 <212> DNA <213> Solanum tuberosum <400> 33 cagacagttt ttaagccgca gatcaattgc agtgacgcca caatcgaata gccatgggga 60 gaagacctgc aagatgttat cgtcagatta agaacaagcc ttacccaaaa tcaaggtttt 120 gccgtggtgt gcctgatcca aagatcagga tttatgatgt gggcatgaag agaaagggag 180 ttgatgagtt ccctttctgt gtgcatttgg tcagttggga gaaggagaat gtctcaagtg 240 aggcacttga agctgcccgt attgcttgca acaagtacat gaccaagtct gctgggaagg 300 atgcttttca cctaagggtc agggttcatc cattccatgt tttgcgaatt aacaagatgt 360 tgtcgtgtgc tggagctgat agactccaaa ctggtatgag gggagctttt ggtaagcccc 420 agggtgtttg tgctcgtgtt gcaattggtc aggttcttct atctgttcgc tgcaaagatg 480 gaaacagtaa ccatgcccaa gaggctctgc gccgtgccaa gttcaagttt cctggacgtc 540 aaaagatcat tgtcagcaga aagtggggat tcactaagtt cagccgtact gattatctga 600 aatacaagtc agagaaccgt attctcccag atggtgtgaa tgctaagctt cttggcaacc 660 atggtcgact tgctgcacgt caacctggaa gggccttcct tacatctgct tagggaattg 720 aagtggcata ttctcacaaa tagcacattt agttttctag tctgttgaat gaaactttgt 780 tacttgaatt tctgtctttg catgaacttg atcagtagta tccaattgaa catttttgaa 840 attatgttct gtattcgaaa aaaaaaaaaa aaaaaa 876 <210> 34 <211> 1262 <212> DNA <213> Solanum tuberosum <400> 34 gactgggcct gcagtgtttt tgatccgtca agttgactta gaattgattc atctctttct 60 ccggtgactc ttcgccgatc accggcgaat ttacgccgat ttttgccaga gagagaagat 120 ttgaaacatc cagtagagag aaaaaaaaat gccttcacag aagattgaaa gtgggcatac 180 tgacactgtc catgatgtgg caatggatta ttatggtaag cgtctggcaa cagcttcttc 240 agactgcacc attaagataa caggggtcag caattcgtct tcgcagcaac tggctactat 300 aagtggccac caaggaccag tgtggcaagt agcttgggct caccctaagt ttggttccct 360 ccttgcttct tgttcttctg atggaaaggt aattatctgg aaggaaggta ctcaaaatga 420 gtggagctta gcacgtgttt tcgatgacca taaagcttca gtcaatgcaa tagcttgggc 480 tcctcatgaa ttaggcctct gtttggcttg tggatcttct gatgggaaca tttccatttt 540 cacagcaaga tcagaagatg tttgggagac atcacggatt gatcaggctc atccagtagg 600 tgttacatct gtttcgtggg cgccatcaac agctcctggc tctcttgtag gctctgatct 660 gcttaatccc gtgccgaagc ttgcatctgg tggctgtgac aatacagtta aagtgtggaa 720 gctgtttgat ggaacttgga agttggattg cttcccagtt cttcaaatgc acactgattg 780 ggtgagggat gttgcttggg cccctaactt ggggctacca aagtcaacta tcgcgagtgc 840 ttctgaagat ggaagagtga ttatatggac agttggtaaa gaaggtgatc aatgggttgg 900 taaagtgttg aaggattttg gtgcccccgt ttggagagtt tcgtggtcac taacaggaaa 960 catattggct gtggctgatg ggaataacaa tgtgacattg tggaacgaag cagtagatgg 1020 tgaatggcaa cctaacaaca gtagatacat aaagttagac acatttataa cccttgagtc 1080 cagaatgtta catcatatta acaaaactct agctattttg caaatataga ttttgcttca 1140 catatcattg tgctatataa cacaacgatc gatgtggtgt ttgacggaag aacagatatg 1200 tatgagcatc tttgatatac aatttaagga taataatcct gcgaaaaaaa aaaaaaaaaa 1260 aa 1262 <210> 35 <211> 709 <212> DNA <213> Solanum tuberosum <400> 35 gctccgaaac acagctaaac ctcaacccta gctcactgtc aaaatgccgc caaagttcga 60 tccatctcag gtcgtggagg ttttcgtccg agtcaccgga ggtgaagtcg gagcagcgag 120 ttcactcgct cccaaaatcg gtccactcgg tctttctcct aaaaaaatcg gagaagacat 180 cgcaaaggag acctcaaagg actggaaggg tctccgagtg accgtcaaac tcacagtgca 240 gaatcgtcaa gctaaggtat cagttgttcc ctccgccgcc gcacttgtca tcaaggcgtt 300 gaaggagccg gaacgtgaca ggaagaagac gaagaacatc aagcataacg gtaacatctc 360 actggatgac gtcattgaga ttgctaaggt gatgcagtct agatctatgg cgaaggattt 420 gagtggaact gtgaaggaga tcttgggaac atgtgtatct gttggttgta cggtagatgg 480 aaaggatcct aaggatttgc agcaagagat tactgatgga gatgtggaga ttcctcagga 540 ttgatgagat aagagggaat tatgaactga tgattccatt gatggaggtt atgttctgat 600 tttgttctta tattaatttt tatgttattt tgctttgagt atgttatcga ggataatttt 660 gatataatta tgacgagtaa actttgggta aaaaaaaaaa aaaaaaaaa 709 <210> 36 <211> 504 <212> DNA <213> Solanum tuberosum <400> 36 ggggtgatag gaaatctacg ggaagaagaa gaagaagaag aagaaaagaa atggctgtaa 60 tggggaagtt gagaatgttc gtggttcagg aaccagtcgt agctgcctct tgtctcatcg 120 ctggattcgg cctcttcctt cctgcggttg taaggcctat tctggactct tttgaatcat 180 ccaagcaagt tccggcacct cctttaagcg atgtagttgc gggcatgact ggtaaaaaac 240 aagaataaga tacttgtgag gttttagttt gaaccagctc cttgtaggct tattccattc 300 ctcttgtttc atcctgatca gaaatgaaaa taaggtcttt tttggtggat actttaaatg 360 ttttgtgtaa ggggggagac cctcctcatt tgtaagcact tgacaagagc cagtgctact 420 tggattcaaa taccgaattg tcttcagtta taacctaatt aaatgcatct gaagcttatc 480 attaaaaaaa aaaaaaaaaa aaaa 504 <210> 37 <211> 677 <212> DNA <213> Solanum tuberosum <400> 37 ggatggtacc tgttgaaatg tattggtaac agaatgcgag acagtaaatc cggaggatcc 60 attgtattta tgacctcgat cattggtgct gagagaggaa tatatcgagg agctgccgct 120 tatggttcat gtgcggctgg aattcagcag ctggttaggt tatctgcaat tgaattgggg 180 aagcaccaaa ttagggtgaa tggcataatg cgtggcttgc acctcgagga tgagtttcct 240 ttgtcagtgg gtaaggagag agcagagaag ttgaccaagg aagcagcgcc tctaaatcga 300 tggcttgatg ctaaaaagga tctggcttcc actgtcatct atttaatcag tgatgattca 360 agatacatga ctggaacctc catctttgtt gatggtgccc agtctctagt gaggccgcga 420 atgcgctcat acatgtgaag tttctaaatt atctatacat gaatggagcc atagacactg 480 gaattatatt gtagtattgt gtaaaagcac aagtatcagg ggaagtacta taagctgtct 540 ggattttctt ctattgtatt tggtttaagt tttcaataag gatgacattc ttctttctcc 600 tcctcatttc tgttgtgatt tagcttaaac tttaatctat tctaagtgag aatttgttgc 660 aaaaaaaaaa aaaaaaa 677 <210> 38 <211> 225 <212> DNA <213> Solanum tuberosum <400> 38 atggagaaga agaaaatgaa tgcagtggca gctatggcta tggtgttgat gatcctttta 60 tcggccaaca tggacatagc cggtgttgct gctcaaggcg tcaattgcta tgataactgc 120 aacaccggat gcgccggtct cccatccaag gagtatctga aatgtgataa gaagtgccac 180 aaaaggtgtg gagatgatga caaaattgat ggaaacgttg gttga 225 <210> 39 <211> 631 <212> DNA <213> Solanum tuberosum <400> 39 gtgccacatc accaaaggca atggttgatt ttgttcaaga aataatggga gataaaacag 60 aaatcaaagc tctatccaca actcatttct caaattcaac tcccctgctt caaaagtata 120 ttattttgga tactccagaa gaagtagaaa ctcctaagat ggtggcatgt cataccatgc 180 cttacgctta tgcgattttc tactgtcatc acacagttag caagagcaaa gtgttcaagg 240 tttcattagg tggcgaaaat ggtgataaag tgaaagcaat tgctgtttgt cacttggata 300 cctctgaatg gagtccatct cacgtatctt ttcgagtgct cggtatatca tcaggaacat 360 gcccaatttg tcactttttt ccatcagata atctactctg ggttccaaaa ttcgccattg 420 cacaagtgat ttgaaggagc actctctcta tgttatcagc aactttgtgt gctactgttt 480 gattactaca tgttattgtg aattagtaat attttcatgc atcttttctg tactgtttca 540 gtgtgcctct tgttttgctc acttttcaat ggtgagatat aaataaaact agtaaatatg 600 attttctgat tgaaaaaaaa aaaaaaaaaa a 631 <210> 40 <211> 819 <212> DNA <213> Solanum tuberosum <400> 40 ggaccaacat cttcttcccc tttttcccta tttctcactt tctctttctt gttgcctctt 60 tcttaggaat actcttttgg tcacctcaaa gaacctttct ttactgttgt atttctgaat 120 gttgccagaa atggggtcag aaggatcatc aagagttgta gtcccaagga atttcagatt 180 attggaggag cttgaaaggg gagaaaaagg aataggggat ggaactgtca gctatgggat 240 ggatgatgca gatgatgtgc acatgcagtc atggacagga actatcattg gccctcctaa 300 tactgtacac gaagggcgca tctatcagct gaaactattt tgtggaaagg agtatccgga 360 tcagccacct ggtgtaaggt tccagtccag gattaacatg agctgcgtaa attatgaaac 420 tggtgtggta gagccaagtc atttccccat gcttgctgac tggaagagag aaaacacaat 480 ggaggatata ttgatgcagt tgaaaaagga aatgacgtct ccccaaaatc gaaaactagc 540 tcaaccttct gatggcaatg aagagggccg ggtggatcaa aaaggcctag ttgtgaaatg 600 ctgcattatg taagttttga caaaatactg aaagataata atgttgtata taaccttgat 660 atgtagacga ttcgagagta tcgtagcatg tataagtatg tgtatgtttt gtatttctta 720 ttgaatcctc cttaaattat gggatggatt gatgtttaat gccaattatc caactctctg 780 aataaggcta aaaaaaaaag aaaaaaaaaa aaaaaaaaa 819 <210> 41 <211> 509 <212> DNA <213> Solanum tuberosum <400> 41 gatcacacaa ttatttgaga gaaaataaaa taaaagcaaa atggagggaa agagtatgct 60 caagttatct catgtgcttg ctttcttgct tcttgcatca ctttttcaat cactgatggc 120 aagagatttg atcagtgatg gcatagaagt actgcaactt ccagtggaaa atgatggcga 180 atttgtattt tgcccaggta agcaatcatg gcctgaactt gttggaaagt cagcaggata 240 tgcaaaacaa gtaattgaga aggaaaattc catagttcat gaagttaggc ttctatttcc 300 tggtatggtt aagccactta attatgtttg tggcagagtt tttctggttg ttaactttaa 360 actcgttgtt caagttactc ccagtatggg ttagtaacat tatgttatga aaattaaata 420 aagtggagat attaaatata atgtcttcat gtactctact atttcaacat aaataaaact 480 aatgttcctc aaaaaaaaaa aaaaaaaaa 509 <210> 42 <211> 168 <212> DNA <213> Solanum tuberosum <400> 42 atgcctactc gttacacact ggatgttgat ttgaaggaag ccgttactgt cgattgcctt 60 cagtcacgtg ataagaaggt tactgctgct aaggaggcca aggctaagtt tgaagagcga 120 ttcaaaaccg ggaaaaatcg ctggttcttc actaagctta gattttga 168 <210> 43 <211> 552 <212> DNA <213> Solanum tuberosum <400> 43 atgtttcatt tgcggcgatc acaatttttg caagttttca ataatagtcc cgatgagaca 60 gcatatttcc ggatgctgct ctaccgagag agtataagca atgctgttgt tatgattcaa 120 cctacattaa tggcattttc attcaataca ctgccttctc cagctttact ggatgtggca 180 tcaattgctg ctgatcgcat tcttttgttg gattcgtact tcagtgttgt catattccat 240 ggaatgacaa tagctcaatg gagaaatttg ggataccaga accagccaga gcatcaggca 300 tttgcgcatc tattgcaagt tcctcatgac gaggcccaag cactcatcca ggagaggttt 360 cctgttccta gacttgtagt gtgtgatcag catggttccc aggcaagatt tcttttggca 420 aagttgaatc cctcagccac atacaataat gcaaacgata tggcagcagg ttcagatgtg 480 atattcactg atgatgtaag tctccaagtt ttcttccagc atcttcagcg actggcggtg 540 caatcttctt ga 552 <210> 44 <211> 689 <212> DNA <213> Solanum tuberosum <400> 44 atgttacctt taaggcagag tgctagttca tctgcatcag atgatgatca aaggtatgca 60 ggaatggatg agaagaagag gaaaaggatg atttccaaca gggaatctgc gaggcgatcg 120 aggatgaaga agcagaagct tctgcaagat ttgacagggg aagtgagcaa attacagtct 180 gcaaataaga atattgtgtc taagattgac gagacaacag aaagatatgc aatttgtgct 240 gcacagaata atgtgttgag ggcacaggca atggaattga ctgataggct caggtattta 300 aatgatgtta ttgacagtac tggtttggct gctggtgctg ctgatccttt gttgaagcca 360 ttgcagaatc cttgtccaat gcagcctatt gcttcttcag gattgtttaa attttaatcg 420 tcgttgtggc ttgtttggat agctttgctt catagtccat attttagtag agtttccttg 480 gatggtatgt catgtagtat gcatttgctg gttttgttta gtgtatgctg tgtctgctac 540 tcttttacca tgtattcaga tcagcttgga gttgtgtgaa atcgatgttt gtttctacga 600 gaataccaat gggttcctat cgtacccgct ctgccgaatt ccacccgtac ggtcatcatt 660 acaatacatg gtagatgttt ctcgtccag 689 <210> 45 <211> 667 <212> DNA <213> Solanum tuberosum <400> 45 gacccaacac tcaaaacaaa tacatttgta gtattatatc atcctctact atttctatcg 60 atctttactc aatttttgta acaatggaaa tgtttggcaa aattgcatgc tttgtgcttt 120 tgtgcatggt ggtggttgca ccccgtgcag aggcactgag ctgcggcgag gttacatctg 180 gcttggctcc ctgcctccct tatcttcaag ggcgcggtcc tattggaggg tgttgtggtg 240 gtgttaaggg tctgttgggt gcagccaaga ccccagaaga ccggaagaca gcatgcactt 300 gcctgaaatc ggcagctaat tctattaagg gcattgatac gggaaaagcc gctggtctcc 360 ctggagtttg tggagtcagc attccttaca agatcagccc ttccactgat tgctccaagg 420 tccagtaagg ttgatgaaat taaagctaag tctatatata gcttgcggag atgaagaata 480 agatggatat atatcgatcg agtttgatcc atcattatat atgttgtctc ttttcttttg 540 tatttgtgtt tgttggagta cttatatatt gtcgagtctt gtaatgaaca ttagtggttg 600 cttgtgttac gacccaatct tcatatagta atacatgata ttttactaaa aaaaaaaaaa 660 aaaaaaa 667 <210> 46 <211> 738 <212> DNA <213> Solanum tuberosum <400> 46 cagggggaaa attagggttt ggcatctctc gccgtcagtt gattttcctt agtaacgatc 60 aatcatggcg gagcagacgg agaaggcgtt tttgaagcag ccaggtgagt ttctaagctc 120 taagaagacc gggaaaggaa agaggccagg aaagggaggc aatcgatact tcaagagtat 180 cggtttagga ttcaagactc ctcgtgaggc tactgaaggt acgtatattg acaagaaatg 240 cccattcact ggcaatgttt ctatcagagg tcgtatcctt gctggtacat gccacagtgc 300 taagatgaac agaaccatca tcgttcgtcg caactaccta cattatgtca agaagtatca 360 gagatacgag aagaggcact ccaatattcc agctcacata tcaccgtgct tccgtgtgaa 420 agagggagat catgttacta ttggacagtg caggccttta tccaaaaccg tgaggttcaa 480 tgtcttaaag gttattccag ctggttctgg tggtggcggg aaaaaagctt ttaccgggat 540 gtaatcttcg tgcctccatt tcaaattttg tttcttgtag gaatattatg tcaagacttt 600 tctgctttta ctgagttatc ttcaaatttt taaacttaat gcttagtaag ttatgtttaa 660 ttaagcctct gaactagctt ataaaatgtt gtttgattga accttataca aaaaaaaaga 720 aaaaaaaaaa aaaaaaaa 738 <210> 47 <211> 726 <212> DNA <213> Solanum tuberosum <400> 47 caggggattc accctttctc tctctagaaa agaagaaccc ttagcgtaaa ctctgcgaac 60 cgaagaaccc caaaaggctt tcaattcaat ggctaacagc aatcttcctc gaagaattat 120 caaggaaact caacgtcttc tcagcgaacc tgcgcctggg ataagtgcgt cgccgtctga 180 agaaaatatg cgatacttta atgtcatgat acttggtcca acacagtctc catatgaagg 240 aggtgtcttc aagctcgaac tatttttgcc tgaagagtac ccaatggctg ctcctaaggt 300 tcgattcctc accaaaatct accatccgaa catcgataag cttggaagga tatgtcttga 360 tattcttaaa gacaaatgga gtcctgctct tcagatccgt acagtacttt tgagcattca 420 agcacttttg agtgctccaa atccagatga tccactctct gaaaacattg ctaagcactg 480 gaagtcaaat gaagttgaag ctgttgaaac ggctaaggag tggacacgcc tatatgctag 540 tggtgcatga agatatagca acgaaatatt caaaataaca aaaatcatgg aatgtaatca 600 atattgactg gcttatcaat atgacgcccg gatagcaccc ttgaaattgt ataattgaac 660 atgacaccct ttggtttttt aatgttcagg aatgaacttt gttacaacaa aaaaaaaaaa 720 aaaaaa 726 <210> 48 <211> 567 <212> DNA <213> Solanum tuberosum <400> 48 cagggggaat atgctcaagt tcttcgtatg cttggaaatg gacggtgtga agctatgcgt 60 attgatggga ctaaacgtct ttgtcacata cgtggtaaga tgcataagaa agtttggatt 120 gccgccggtg atatcgtcct tgtcggactc cgtgattatc aggatgacaa ggctgatgtt 180 attctgaagt atatgccaga tgaggcaagg ttgttgaagg catatggggg gttgccagag 240 aacactaggc tcaatgaggg tattgctaat ttggatgaag aagatgagaa tgctgctgat 300 gattacattg agtttgaaga cgaagacatc gacaaaatct aaaactcctt atctaaacag 360 tgctgtcact gggggttcct gttggcctca caaatatttc cttaagagtt tgagttcaat 420 acaaaaagaa agagagagac cttatatgta ccattttaat atgattgaac tttgttcact 480 agtatttctg tatgaagatt tatgctttga tgaagcagat gcaataatag ccacctgttc 540 tctattctaa aaaaaaaaaa aaaaaaa 567 <210> 49 <211> 657 <212> DNA <213> Solanum tuberosum <400> 49 atgaagtgtt tatttttgtt atgtttgtgt ttggttccca ttgtggtgtt ttcatcaact 60 ttcacttccc aaaatctcat tgacctaccc agtgaatctc ctctacctaa gccggtactt 120 gacacaaatg gtaaagaact caatcctaat tcgagttatc gcattatttc cattggtagg 180 ggtgccttag gtggtgatgt atacctagga aagtccccaa attcagatgc cccttgtcca 240 gatggcgtat tccgttacaa ttccgatgtt ggacctagcg gtacacccgt tagattcact 300 cctttatctg gaggtatatt tgaagatcaa ctactcaaca tacaattcaa tattccaaca 360 gtgaaattgt gtgttagtta tacaatttgg aaagtcggaa atctaaatgc atattttagg 420 acgatgttgt tggagaccgg aggaaccata gggcaagcag ataacagcta tttcaagatt 480 gttaaatcat caaaaattgg ttacaactta ttgtcttgcc catttacttc tattatttgt 540 ctacgttgcc ctgaggatca gttttgtgca aaagtgggtg tagttattca aaatggaaaa 600 aggcgtttgg ctcttgtcaa cgaaaatcct cttgatgtct tattccagga agtctag 657 <210> 50 <211> 783 <212> DNA <213> Solanum tuberosum <400> 50 caggggcaca aagctaaggc acacacaagc taagccatat tcatcaattc cttagaagcc 60 acaaaagata atataattat ttttctccaa gtatggcttc taaaggaatc gctataattt 120 gcatggcttt agttccaatt atcatgacca tgatttcagt acaagtagcc atggctcaat 180 ctgattgtac aagtacactt attactatgg cttcatgcct tagctttgtc accggaagtg 240 cgaaaacgcc atcggcttct tgctgctccg ccctctccgg ggtgttgcag tctaagccac 300 ggtgcctttg tgtcattgtc aatggtggcg gttcgtcgtt aggagtccaa atcaatcaaa 360 ctcaagcact tgcattgcct agtgcatgca atttgcaaac tcctcctgtt agtaaatgtt 420 acgagggtgc accttcagaa ggagctccag attcttcgac tggtatagct atctcaggct 480 caaaggcgtc aggaagcagc agcacctctg atggaagttc tctgaaggct ccgattcgag 540 ctgctgttgg aatcgttctt ttcatggctt cctacgtttc catgatttga agtttatgaa 600 ttttcacgtc gaagtatcat agtactaaat aaaagtagca ttatgatatg ttacatgtta 660 tattggggtg tggtttatta gtgtttgtct ttaagtttgt tgtgtgtctg ttcctttttt 720 attatttgtt tctctatata ttgtaccagt atttgtgatg cagttttaac tttatttcta 780 agc 783 <210> 51 <211> 669 <212> DNA <213> Solanum tuberosum <400> 51 caggggaatt catactaagt agtgagagca aaagaagaaa aaaggtatag gagaaaatgg 60 caagcacagt aatgagctca ttgagcctaa agccaacttt cactttggag aaaacatcag 120 tgaaagggct tccatcactt gctagatctt cttcttcctt caaagttgtg gctagtggtg 180 tcaagaagct taagactgac aaaccctatg gaattaatgg aagcatggcc ttgagagatg 240 gcgttgatgc ctcaggcagg aagcccaagg gaaagggtgt gtaccaatat gttgacaaat 300 atggagctaa tgttgatgga tacagtccca tctacaacac agatgagtgg tctccaagtg 360 gtgatgtcta tgttggaggt actactggct tagccatatg ggccgtgacc ttggttggta 420 ttcttgcagg aggtgctctc cttgtctaca acacaagtgc tttggcacag tagatgttat 480 cctgtgttgt actattttaa gttgtattcg agctcgcatc atgtaatcct ttcaaaaata 540 cttcaaaata tttcttggca tcttgattaa tatgtaattg ctgtgctttt actgtggaca 600 tgatactctt agaggaaaag aaaggggaag aaaacacgga aacaaagtgc catttaatta 660 aataaaaaa 669 <210> 52 <211> 738 <212> DNA <213> Solanum tuberosum <400> 52 cagggggaaa attagggttt ggcatctctc gccgtcagtt gattttcctt agtaacgatc 60 aatcatggcg gagcagacgg agaaggcgtt tttgaagcag ccaggtgagt ttctaagctc 120 taagaagacc gggaaaggaa agaggccagg aaagggaggc aatcgatact tcaagagtat 180 cggtttagga ttcaagactc ctcgtgaggc tactgaaggt acgtatattg acaagaaatg 240 cccattcact ggcaatgttt ctatcagagg tcgtatcctt gctggtacat gccacagtgc 300 taagatgaac agaaccatca tcgttcgtcg caactaccta cattatgtca agaagtatca 360 gagatacgag aagaggcact ccaatattcc agctcacata tcaccgtgct tccgtgtgaa 420 agagggagat catgttacta ttggacagtg caggccttta tccaaaaccg tgaggttcaa 480 tgtcttaaag gttattccag ctggttctgg tggtggcggg aaaaaagctt ttaccgggat 540 gtaatcttcg tgcctccatt tcaaattttg tttcttgtag gaatattatg tcaagacttt 600 tctgctttta ctgagttatc ttcaaatttt taaacttaat gcttagtaag ttatgtttaa 660 ttaagcctct gaactagctt ataaaatgtt gtttgattga accttataca aaaaaaaaga 720 aaaaaaaaaa aaaaaaaa 738 <210> 53 <211> 528 <212> DNA <213> Solanum tuberosum <400> 53 atggctgatc agtacgaaca gaacaagcca tcagttgaag agactgttgg tgccaacgtg 60 gaggctactg atcgtggttt gtttgatttc attgggaaaa aaaaagagga aaaaccaagt 120 catgctcatg aagaagaggc aatttcatct gagttttgtg agaaagttaa agtaagtgaa 180 gaagaacaca aggaggagga aaagaaaaaa gagaagaaac ttcatcgatc aagtagcagc 240 tctagtagct cgagtgacga ggaggaagaa attggagagg atggacagat aatcaagaag 300 aagaaaaaga agggattgaa ggaaaagatt aaggaaaaaa tatctggtga tcacaaggaa 360 gaagtgaaaa cagaggatac ctcagttcca gttgagaaat acgaggaaac agaggagaaa 420 aaaggatttc tagacaaaat taaggagaaa ttgccaggtg gtggacataa gaagacggag 480 gaagtggcgg cgccaccacc accaccaccg gctgcggtgg agcattag 528 <210> 54 <211> 826 <212> DNA <213> Solanum tuberosum <400> 54 cagggacaga cggacacaga gctgcttgag agggagagag agagagagag agagagagga 60 cggcactgaa aatggtgacc ttcaaatttc atcagtacca ggtggtgggt agagctcttc 120 catcagagac cgatgaacac ccaaagatct atcgcatgaa actttgggct accaatgagg 180 tccgtgcgaa atccaagttc tggtactttt tgaggaagct gaagaaggtt aagaagagca 240 acgggcagat gcttgctatt aatgagatct ttgagaagta ccctactaag atcaagaact 300 acggcatttg gttgcgttat cagagtcgca ctgggtacca caacatgtac aaggagtacc 360 gtgataccac attgaatgga gctgtagaac agatgtacac agagatggct tcacgtcaca 420 gggtccgcca tcactgcatc cagatcatta agacagccac tattccagct aaactgtgca 480 agagggagag caccaagcag ttccatgact ccaaaatcaa gttccccttg gtgttcaaga 540 aagtcagacc acctactagg aaactcaaga ctacatacaa ggctacaaaa cccaacttgt 600 tcatgtaaca tgttaggtca taagtttatt gagaagcaac ttagaattca tgcaaatttt 660 gttgcactag ttcgccagtt tggcactaat ggaaaacctt tatgtccctt tttccttata 720 actctaggta tcagacagtt aaagtttctc ctttctgagt tttaaatttg tttgcttcaa 780 cagttccagt tcaagtttta tgtgctctct caataaaaaa acaaaa 826 <210> 55 <211> 261 <212> DNA <213> Solanum tuberosum <400> 55 atgagcggag gtggaatgga gtctatggca cggccaactc cgccggaagt gggcggtgga 60 ataaacagcc agcacgacgg agaacggtgt gggtatgggt tccatatgcc attgcattac 120 ccaagataca cacgagctga atatgaagca atgccagaat ggaaagtaga ttgcttactt 180 gctcaatatg gacttcctag taatggagat gttaatcaaa agagaaagtt tgctatggga 240 gcttttctat ggacctatta a 261 <210> 56 <211> 775 <212> DNA <213> Solanum tuberosum <400> 56 caggggcgac tcgcggtgga gacgaacaac ctccaaaact ggaaattggt gcctaaagaa 60 tgtacaaact atgtaggtca ttacatgtta ggcaaacaat atcgccacga ctgtgaatat 120 gtagccaagc aggctattga gtatgccaaa agcctcaaac ttagtggaga tggaatggac 180 gtttgggtat ttgatattga tgaaaccact ctctcaaatt ctccttacta cgctcgatct 240 gacgttgctt ttggagctat agcgtacaac gctacaaagt ttaatgagtg gaccgcggag 300 ggaaaattgc cagccattcc atcgatactg ggtctataca agattgtgtt gtctttgggg 360 atcaagcccg ttttcattac agggaccaga gacaatttca aacaagtgag gattgctaat 420 ctcaagaaag ctggttatac caactgggca gcgctcatac taaagggaga aaatgattcg 480 ggatcagcag tgaaattcta atcaagcaag agaacagcat tggtgaagac tggatataga 540 attgttggta atattggaga tcaatggagt gatcttattg gaaaaaacgt tggagctcgt 600 acttttaaac tccctgaccc tatgtactac attggttgac tctaaccaaa ttttaatcaa 660 caaaggtggg gtaccttttt tattttgaca tgtgagatat tataagctca ctgttcctaa 720 tccccatcac tttgctataa ataacgactt ccttcgaaaa aaaaaaaaaa aaaaa 775 <210> 57 <211> 996 <212> DNA <213> Solanum tuberosum <400> 57 atggctttgt ctctgtttct ctacttgccg aagcttgatg aaatggtgtc gtgtgagtac 60 agagagttgc cccacccgat tcagattccg ggttgtaccc cgatccacgg aaaggatcta 120 ctcgacccgg ttcaaaaccg gaaagatgaa tcttacaaat ggcttcttca tcatgcgaag 180 aggtatggaa tggcagaagg gataatagca aacagcttta agaatttgga aggaggagcc 240 attggagctc tacagaaaga agaacccggt aagccaaccg tttacccggt tggcccgctt 300 attcagatgg attcgggtag tagggttgac gggtcggagt gtctgacgtg gctggatgaa 360 cagccacgtg gctctgtgtt atacatatcg tacggtagtg gtgggaccct ctctcatgag 420 caacttattg aagttgcaaa aggcttggaa atgagtgaac aaaggttctt gtgggtagtt 480 agatgcccta atgacaaaat agctaatgct acttttttta atgtccaaga ttcaactaat 540 cctcttgagt ttttaccaaa agggttcttg gaaaagacaa aagggtttgg tttagtggtg 600 cctaattggg ccccacaagc ccgaatccta agccatgagt caacgggtgg gttcttgact 660 cactgtggat ggaactcaac tctggagagt gtggtccatg gggtaccact tatagcttgg 720 ccactctatg cggaacaaaa aatgaacgcg gttatgttaa gtgaggatgt aaaagtggca 780 ctaaggccaa aagtcaatga agaaaatggc atagtgggaa gattggaaat tgctaaagtt 840 gtgaaaggat taatggaagg tgaagaaggc aaaggagtgc gcagtagaat gagagacctt 900 aaagatgcag cagccaaagt gcttagtgaa ggtggttctt ctacaaaagc attagctgaa 960 ttggctacta gattgaagaa aaaaagtgtc aaataa 996 <210> 58 <211> 529 <212> DNA <213> Solanum tuberosum <400> 58 ttgcctgcct tagcagtggt atcacgcaga gtacagggac atcctccaag aagattagaa 60 aataaaagca aacaatcaag catgggtagt aacaaagttg gtgtcctcac tctcctcctc 120 ctatgtgttt ttctagtggg aagcaattat gtggaagctc agatttgtcc tcaattttgt 180 gagccaaatg tggattacat gacttgttcc tcttcaggaa gcacaatact tcgtccaaca 240 tgcatcaact gtagccaagc aagaggaagg ggatgccaac ttttccgtag agatggctct 300 gcaatatgta actaataaat cacctttgag atttatcaaa ggggatatat taatagtttc 360 taataaatta tgatcatata tatgtgttta aaataaattc tgagtttatg atttactcag 420 atgaccaata ttatatataa ataatggatt tttagtgttt gtctaaaaaa aaaaaaaaaa 480 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 529 <210> 59 <211> 1229 <212> DNA <213> Solanum tuberosum <400> 59 ggtctgaact cagaatttga catttatagt tttaaaaaaa atactatcta ttatcttctc 60 ttccatgttg aatttttgaa ctataaaaac catttgaaaa tgcattcatc atctgtttta 120 tctcctccaa attgctcact actgaagaga tcacacagaa atcaaatcga gaaattgtct 180 ttagcgccat tgaagaatct tccgttctgt ggactgagaa atgagatttt agcgtttgag 240 ttttcgaagc ttaatcgatg tgagactcag agagtgagga attttctaag gaataaaata 300 gtatcagctt cggtggctga aaatggtaat gctccgacgt cgtttgatta tgatttagta 360 ataattggtg ctggtgttgg tggccatggt gctgcattac atgctgtgga gaagggtctt 420 aaaacagcta tcattgaagg agatgtaatc ggcggaactt gtgtaaacag aggttgtgtt 480 ccttctaaag cacttcttgc agttagcggt aggatgcggg aacttaagaa tgagcatcac 540 ttgaaagctt tgggtataca ggtttctggt gccggctatg acaggcaagc agttgctgat 600 catgctaata atcttgcctc gaaaattcgt aataacttga caaactctct aaaggcactt 660 ggtgtggaca ttttgacagg agtgggtact ataacgggtc ctcaaagggt gaagtatgga 720 aaagttggtt tttctggcag tgaagttacc gcaagggata taattatcgc taccggttct 780 gtcccttttg ttcctagagg cattgaagtt gatgggaaga ctgtgattac cagcgaccat 840 gctctcaaac tggagtttgt acctgaatgg atagctatag tagaagtggt tatattggac 900 ttgaatttag tgatgtgtat accgcacttg gtagtgaggt tacttttatt gaagctctag 960 atcacttatg cctggatttg atccagaaga attagtaagt tagctcaaag attctggtaa 1020 tccaggaaaa ttgattatca cacactggat gtttgccacg aagatcactc ccgcaagaga 1080 tggggagaac agtgtttatg actacctgac gcccgacata agagttaaga acctttggag 1140 gtgatgcacc tcatggcact cgagctcaag ttctatgctg gaattacctg tgacacacag 1200 gtatttctgt cgtgagcgac tcgcacagt 1229 <210> 60 <211> 1169 <212> DNA <213> Solanum tuberosum <400> 60 atgaatactc acaaagctca ttgcttgatc ttgccatatc cagtccaagg tcatatcaac 60 ccaatgctcc aattctccaa acgtttacaa tccaaacgtg tcaaaatcac tatagcaccc 120 acgaaatcct tcttaaaaaa catgaaagaa ttgccaactt cagtgtcaat cgaggccata 180 tctgatggct acgacgatga tggcatcaac caagcaaaaa catacgaggc ctacctaaca 240 cgattcaaag aagttggctc ggatactctg tctcagctta ttcaaaaact ggcaaatagt 300 ggatgccctg taaattgcat agtatatgat ccattccttc cttgggctgt tgaagtggca 360 aaaaaatttg gattagtaag tgctgctttt ttcacacaaa attgtgctgt ggataacatt 420 tattaccatg ttcataaagg ggtaataaaa cttccaccta ctcaacatga tgcaaaaata 480 ttaattcctg gattatcatg tacaattgag agttcagatg tgcctagttt tgagtcaagt 540 cctgaatcag ataaattagt tgaactattg gtgaatcaat tctcaaatct tgagaaaact 600 gattgggtat taatcaacag cttttatgaa ttggagaaag aggttattga ttggatgtcc 660 aagatatatc caatcaagac aattggacca acaataccat caatgtactt agacaagaga 720 ctacatgatg acaaagagta tggtcttagt atcttcaaac caatgacaaa tgaatgccta 780 aattggttaa atcatcaacc aattagctca gtgttgtatg tatcatttgg aagtttagcc 840 atagtaaaag ctgagcaaat ggagattggc atggggtttg atgaatagca cagaacttct 900 tgtgggttgt tagtccactg agaatctaac ttcccagagc ttttagagga ctagaattga 960 caagtgcata taagcctagt ggggtcatgt gtccacaaat tacagtgttg acatgaatcc 1020 gataggtgtt ttctgacgca ttgtggatgg atcgactctg agcaattatt tgggagtgcc 1080 aatgtgtata tggccaatgg tcgatcacgc catcagcttg gcagtttgat ggactagcca 1140 caagtgacag ggagtttaaa ggtttttta 1169 <210> 61 <211> 996 <212> DNA <213> Solanum tuberosum <400> 61 gacaacatct tctttaattt cttctcttcc atcaaaatga ggtcatgttt cactattctc 60 ttgttatttt cagctatagt agctgtggca ttggcctcta atgttgaaga ggtgataagc 120 caagtagtag aaattcaccg tctaaggcca caaactggtt ctgctggtta taccgtccca 180 catttggact gtcttagctg gcgactcgcc gtggagacaa acaacctcca atactggaaa 240 ttggtgccta aagaatgtac aaactatgta ggtcattaca tgttaggcaa acaatatcgc 300 cacgactgtg aatatgtagc caagcaggct attgagtatg ccaaaagcct caaacttggt 360 ggagatggaa tggacgtttg ggtatttgat attgatgaga ccactctctc aaatcttcct 420 tactacgctc ggtctgacgt tgcttttggg gctatagcgt acaactctac aaagtttcat 480 gagtggatcg cggagggaaa agcgccagcc attccatcgg tactgggtct atacaagatt 540 gtgttgtctt tggggatcaa gcccgttttc attacaggga cgagagaaaa tttcgaacaa 600 gtgaggattg ctaatctcaa gaaagttggt tataccaact gggcagcgct catactaaag 660 ggagaaaatt attcagggtc agcagtgaaa tttaaatcga gcaagagaac agcattggtg 720 aaggctggat atagaattgt tggtaatatt ggagatcaat ggactgattt aatcggagaa 780 aacgttggag ctcgtacttt taaactccct gaccctatgt actacgtagt tgactctaac 840 caaattttaa tcaacaaagg tggtggggta gctttttatt ttggcatgtg agatattata 900 agctcagtac tgttcctaat cccccatcac tttgctataa ataatgactt ccttcatatt 960 ttcacgaaga aacaagagaa aaaaaaaaaa aaaaaa 996 <210> 62 <211> 567 <212> DNA <213> Solanum tuberosum <400> 62 cagggggaat atgctcaagt tcttcgtatg cttggaaatg gacggtgtga agctatgcgt 60 attgatggga ctaaacgtct ttgtcacata cgtggtaaga tgcataagaa agtttggatt 120 gccgccggtg atatcgtcct tgtcggactc cgtgattatc aggatgacaa ggctgatgtt 180 attctgaagt atatgccaga tgaggcaagg ttgttgaagg catatggggg gttgccagag 240 aacactaggc tcaatgaggg tattgctaat ttggatgaag aagatgagaa tgctgctgat 300 gattacattg agtttgaaga cgaagacatc gacaaaatct aaaactcctt atctaaacag 360 tgctgtcact gggggttcct gttggcctca caaatatttc cttaagagtt tgagttcaat 420 acaaaaagaa agagagagac cttatatgta ccattttaat atgattgaac tttgttcact 480 agtatttctg tatgaagatt tatgctttga tgaagcagat gcaataatag ccacctgttc 540 tctattctaa aaaaaaaaaa aaaaaaa 567 <210> 63 <211> 1181 <212> DNA <213> Solanum tuberosum <400> 63 gcttttcgtc aaactcctcg ttctcctttc tcttcattgc ctgtttcgtc gcagcgattt 60 ctaaggcgag tagaacgcag gagtttattc taggctgcga tggcagataa ccagcaccca 120 actgtttatc agaaggtagc tagccagatg catctgagct ccagtctttc acaggatgtc 180 catgctcact atgggggcat tcaaaggcct gctctctccc agagacgttt tccgtatggc 240 aattactcta atgccggact acaaacatgc caagccacac aggatctctc attgattgct 300 gcaaatgcgt caccagtgtt tgtgcaagct ccacaagaga aaggacttgc agcatttgcc 360 actgacttcc tcatgggtgg tgtttctgct gctgtgtcaa agactgctgc tgcccctatt 420 gagcgtgtga agcttttgat ccaaaaccaa gatgagatga ttaaggctgg tagactctca 480 gaaccataca agggaattgg agactgtttt agtaggacaa ttaaggatga aggatttgct 540 gccttgtgga gaggaaacac cgctaatgtc attcgttact tccccactca ggcattgaac 600 tttgcattta aggactactt caagaggctc ttcaacttca agaaggaccg cgatggctac 660 tggaagtggt ttgcaggtaa tttggcatct ggtggtggtg ctggtgcttc ctctttgctc 720 tctgtttact cccttgacta tgctcgtact cgtcttgcaa atgatgccaa ggctgcaaag 780 aagggaggtg gtggcagaca attcgatggg ttggttgatg tctacagaaa gacacttaaa 840 tctgatggag ttgctggctt gtaccgtggg tttaacattt catgtgttgg tatcattgtg 900 taccgtggtt tgtacttcgg aatgtatgat tcattgaagc cagtgctgtt gactgaaaga 960 tgggaggata gtttctttgc tagctttgct cttggatggc ttatccacca atggtgctgt 1020 cttgcatcgt accctattga cacaagttag agagatgatg atgacatctg ggtggaaggc 1080 agtgagtaca agaagctcgg tcgatgcatt cacatctaga tgagtccaat ctttggttta 1140 agggtgcttg agcctaacgg ttcctctccg tggcgcggtg t 1181 <210> 64 <211> 888 <212> DNA <213> Solanum tuberosum <400> 64 atggggcgag aaggagattg ggagtgtagt agttgcggta ataagaatta tgcattcaga 60 tgtttctgca ataggtgtaa gcagcctcgt ctccttgttg acaacaaaac tcctcatgat 120 tcaaagtggt ttcctcgaat tggcgattgg atctgcactg gttgcactaa caacaattat 180 gcatcaagag aaaagtgcaa aaaatgtgga caaccaaagg aggtagcagc aatgccagca 240 attgcaatcc ctggagcatc tctcccatct catccaaatt attttgccag gacacaagga 300 ggtatggaac aaaggttgaa cattgggttc ttaggacatg gagctctcca acaacttccc 360 ttgagctcta actggtctct aggggaggct ggtcaatacc aaagccagcc tgctgatcgg 420 tatagactgc agcaaactcc tggagttcca tatgcaagcc aaacgaatca gctgcttcca 480 gttccaaatg gatggcgtaa tggtgactgg ctctgcagtt gtggttttca caactactct 540 tctcgagctc agtgcaaaaa atgcaatgct tctgcacctc cagcttcatc gtcttctctt 600 gccagtaccc ctattacagc tcttggaaca aagcgattag catctgagga acttgttcat 660 gattgggaca acaagaggct gaatgcaggg catacatttg ggtatcaaca aagctatcca 720 ggagcagaat ggatggggga ttcaggggga aatcaactaa ctgcaatccc aacgatcttc 780 cctagagaaa ttcaatggtg cttcctttgc aagccaacat gcagattcct cgtgtacagc 840 agcactacac tccttggtaa agggcaaagc atgcgtgatg gggactga 888 <210> 65 <211> 1186 <212> DNA <213> Solanum tuberosum <400> 65 gactctgttc ttctatctct cataaaccct agcagccgcg cccccccccc cccccgaaat 60 ggctacagct gctgccatcc ccaccaccac ggttcaaaca ttcgaaaatg acatgcctac 120 tgacggcggt tccgcggcca tccctctccc cgccgtcatg aaagctccga tccgacctga 180 cgttgtaacc tacgtccacg ccaatatctc caaaaatgca cgacaacctt acgccgtttc 240 gagaaaggcg ggtcatcaga cctctgctga gtcatggggt actggtcgtg ctgtatctcg 300 tatcccccgt gttgctggtg gtggaactca ccgtgctgga caggctgctt ttggtaatat 360 gtgtcgtggt ggtcgtatgt ttgctcctac acagatctgg cgccgttggc atagaaagat 420 tccggttaat cagaagcgat atgcggtagt ttcagctatt gctgcttcct cagttccttc 480 attggttctc gcccgaggac atcgtattga atcagtacct gagcttcctc tggttgtttc 540 tgattccatt gagggaattg agaagacttc taatgctatc aaggcgctaa agcaagtggg 600 tgcttatcct gatgccgaga aggcgaagga tagccatgct attcgtacag gaaagggtaa 660 gatgaggaat cgtcgctaca tttcccgtaa aggtccattg attgtgtatg gaactgaagg 720 ggctaagttg gttaaagcct tccgtaatat tcctggagtg gaaatttgcc atgttgagcg 780 tttgaatctc ttaaagcttg ctcctggagg tcatcttgga cggtttgtta tctggacaaa 840 atcagcttat gagaaattgg atgagattta cgggtcattc gacaagccat ctcagagaag 900 aaggatactt attgccacgt ccaagatggt gaatgctgac cttgctagat catcaactcg 960 gacgagtaca atcggtgtga gactatcaga aggtgtggaa gaggcacatg agaagaacca 1020 ctaagaacct gatgtgctgc tgaactcacc ctatgcaaga ctgcaaagaa tgtctctttg 1080 ctgagctcac ttcagcttga cgaacctgca agaacaacta gggagggtcc tcagctgact 1140 gtcgcatgat tcgatcgtac gatcgaaact ccaaggttgc tccgat 1186 <210> 66 <211> 802 <212> DNA <213> Solanum tuberosum <400> 66 gatgaaatta ataaatagta cactacttag taagtgattt attagtatat ttgaaattat 60 ttaaaatggc gttcgccgtc gcatcgggaa gcttctacat gccggccatg gctacaaacg 120 ccgggcacag ccgtactccg atggccatcg gaattggacg cgtgtcagtt gctaattcgc 180 gcgctccttt cgtttgctta gccagtaaaa aggaggaaat tagtacagat aaggcaccag 240 ctgcagtggg accatattct caaggaatta aggctaataa tttcatttat gtatctggtt 300 ctctaggtct tattcctgag actggagcac tcatatcaga caatgtggag gatcaaacag 360 aacaggctct gaaaaatatt ggggagatac ttaaagctgg tggtgttggc tattctgcag 420 ctgctaagac aacaatcatg ttggctgatc taggggactt ccagaaagtc aatgcaattt 480 atgctaaata ttttccagat cctgcaccag cacgttcaac ttttcaagca gcaggattac 540 cactgaatgc aaaaattgaa attgattgca tagcagtatg ttaaacagat tcaagtcttc 600 aacttccatg gcagctatgg ttttaagttt aaataaagga gatttaagtc tttaattcag 660 agtctcttga tattctgctt tagattcaat ctccattgct gcagagtgca gcaccaataa 720 attctgtaaa agagggctct ttcagtcccc ataataagaa ttcatcgagt gtacgttcaa 780 aataaaaaaa aaaaaaaaaa aa 802 <210> 67 <211> 559 <212> DNA <213> Solanum tuberosum <400> 67 gactcatttt acatcaatca taacacattt gatttctcaa ttttttaatc tcagatcaat 60 catgtcttgc tgtggaggaa actgtggctg tggatctggc tgcaagtgcg gcaatggctg 120 tggaggatgc aagatgtacc cagacatgag ctacaccgaa agcatcacaa ccaccgagac 180 tttggtgctt ggggtgggac ctgagaagac aagcttcggc gccatggaga tgggtgaatc 240 ccctgttgct gagaatggct gcaaatgtgg atctgactgc aagtgcaacc cttgcacttg 300 ttctaagtga acaaactata gttaaaacag agcagagatc atggtgttag ccttctatgg 360 ctgaagaaaa tagttgaaaa atgtgtgtct ctctagtgtt ttagtgtcag tctgttgaaa 420 cagggaaaaa atatgtgttc tttttttccc tgtaataaga aatatagtaa tggaatgtat 480 aagtaaccag taaaatggtt tgtgtaacat gagcatctct gcatctggta attttaacta 540 aaaaaaaaaa aaaaaaaaa 559 <210> 68 <211> 153 <212> DNA <213> Solanum tuberosum <400> 68 atggggtatg gaagagtgga atgggtggtt ctagattgga atgtgaatgc gatcaagttt 60 tatgaagaaa tgggagctca aattatgcag gaatggaggg tttgtaggct gactggtgat 120 gcccttcaag cctttgcaaa tatcaacatt tga 153 <210> 69 <211> 667 <212> DNA <213> Solanum tuberosum <400> 69 gacccaacac tcaaaacaaa tacatttgta gtattatatc atcctctact atttctatcg 60 atctttactc aatttttgta acaatggaaa tgtttggcaa aattgcatgc tttgtgcttt 120 tgtgcatggt ggtggttgca ccccgtgcag aggcactgag ctgcggcgag gttacatctg 180 gcttggctcc ctgcctccct tatcttcaag ggcgcggtcc tattggaggg tgttgtggtg 240 gtgttaaggg tctgttgggt gcagccaaga ccccagaaga ccggaagaca gcatgcactt 300 gcctgaaatc ggcagctaat tctattaagg gcattgatac gggaaaagcc gctggtctcc 360 ctggagtttg tggagtcagc attccttaca agatcagccc ttccactgat tgctccaagg 420 tccagtaagg ttgatgaaat taaagctaag tctatatata gcttgcggag atgaagaata 480 agatggatat atatcgatcg agtttgatcc atcattatat atgttgtctc ttttcttttg 540 tatttgtgtt tgttggagta cttatatatt gtcgagtctt gtaatgaaca ttagtggttg 600 cttgtgttac gacccaatct tcatatagta atacatgata ttttactaaa aaaaaaaaaa 660 aaaaaaa 667 <210> 70 <211> 906 <212> DNA <213> Solanum tuberosum <400> 70 atggaagctg tcactattgc ttcccatttc tcgccggcta ccggaatacg gctatcaact 60 ccggcgagtt gccgtgcctc agctcccaaa cggaatctca gattttctcc tcccacgaaa 120 tcttctctat cgacgagctt tatctcccca ttcgtcggcg gcagtctatt ctcggactta 180 tcgggtcaga gaattcgacc cgattctctt tacccttctt cctcaactgg atttatcccc 240 aaacgtgccg ttgtcactat ggttattcct ttctcagggg acccatcgca ggaacatcct 300 ccagatttag catcttactt gtttaagaat cgaatcgttt acttgggcat gtctctagtt 360 ccatcagtga cggaattgat tctagctgaa tttctttacc ttcagtatga ggatgaggat 420 aagccaatct atctttatat aaattctact gggactacca agggtggtga aaagttgggt 480 tatgagacag aggcgtttgc tgtatatgac gttatgagtt atgtcaagcc acctatattt 540 actctctgtg ttgggaatgc atggggagaa gctgccttgc ttttagcagc tggtgcaaaa 600 ggaaatcgtg ctgcgctgcc ctcatctaca attatgatta agcagccaat tgctcagttt 660 cagggtcaag caacagatgt tgagctcatg cggaaagaag taaataatgt caaagcggaa 720 ttggtgaaat tgtattcaaa gaatactgga aaatcacctg aggagattga agaagacata 780 agacgtccaa aatactttag tcctagtgaa gcagtagaat atggaattat cgatatgttc 840 tatacaatga gagggaataa agatagagga tttgtatctg atctgaagaa ggcccaactt 900 atctga 906 <210> 71 <211> 1216 <212> DNA <213> Solanum tuberosum <400> 71 agagataact agaggtcgtc gtcggctagt ctttgggtca tccaacaaaa cacacttggc 60 cagcttgtat tttagccaat ttccattcat tgctttctct gctgctacta tttccccgat 120 cagaagaatc aaaattaggg tttattgcct gaaaattact agtgccccaa cgccttctcc 180 tactttccct tttagttgct gaaatttaac gcctgatgta actgtatttt aggtttcaat 240 tttgaaatac gagttaattt ctcagatcag ttcttctatt taaaaacctc ataaatggct 300 cttcatgtta ctgctttctc cttcactgga gtttctactt caagtaaagc ttccagaagc 360 tctttgcttt cagtttttcc tgctcctaga agatgcactg tcaaagctgc ttcccagtat 420 ccgcccagca ttcctcgatg taaaattcaa ggaagagcat tgccaagtga caatgggcca 480 ctggagaagg atgaatcttc tcttgttgtg tgctttggag aaatgcttat tgattttgtt 540 ccgactataa gtgggctttc attggctgaa gcccctgcat ttaaaaaggc tcctggtggt 600 gcaccagcta atgttgctgt tggaatttcc cgtcttggtg gttcatcagc tttcattggc 660 aaggttggtg aagacgaatt tggttacatg cttgctgaga ttttgaagga gaacaacgtc 720 aacagtgatg gtatgcgctt tgatccaggt gctcgtactg ctttagcatt tgtaacactg 780 agaaaggatg gagaacgtga atttatgttc tatcgtaacc cgagtgctga tatgttgctt 840 caagaggatg aacttgattt agagttgatc aggaaggcaa agtttttcat catggttcca 900 taagtctgat tacagagcca tgcaaatcag cccatattgc agctgcaaag ctgcgagatg 960 ctggtgtgat cttatctatg atccaattga gcttcattgt ggcatctgca gaagtgctga 1020 gagatcttag catatgggat actgctgata tattaagaat aatggagaga agagattcgt 1080 tttgacacag gaagagattc tttagagtag atatgttgtt cgtagtgtac catccaacct 1140 ccagtattct ctcgtccaag gtcagtgcaa ttaccagatt cctgggacga attaggtata 1200 aagggggacc ggtaac 1216 <210> 72 <211> 470 <212> DNA <213> Solanum tuberosum <400> 72 ctccagcgtt tcttttggta aagaccatct tccgattctt ctctaccgac ccattcaact 60 actttaccat gactcgcgga aatcagaggg aaaaggatcg ggaaagagct gcagctcgtg 120 ctggtaaagg caagaaggga ggcgatgatg gtttgactcc tgagcagcgc cgtgaaaggg 180 atgcgaaagc tctgcaagag aagactgcaa agaaagcagc aaaagaggct gccgggggta 240 gcaatgctgg aactaaggat accaagaaat agaaagctag gtgttgttac ttccattgca 300 gtcagtaatt tgtcattccc ctttgtttgt catgttattc actgtttaaa tgttgtgatg 360 caatacaact taatacgtct ggattgtcaa tccatccggt tttgaactat ctcgtcttcc 420 ttttgatatg ataaactcca cctccaaacc aaaaaaaaaa aaaaaaaaaa 470 <210> 73 <211> 1229 <212> DNA <213> Solanum tuberosum <400> 73 ggtctgaact cagaatttga catttatagt tttaaaaaaa atactatcta ttatcttctc 60 ttccatgttg aatttttgaa ctataaaaac catttgaaaa tgcattcatc atctgtttta 120 tctcctccaa attgctcact actgaagaga tcacacagaa atcaaatcga gaaattgtct 180 ttagcgccat tgaagaatct tccgttctgt ggactgagaa atgagatttt agcgtttgag 240 ttttcgaagc ttaatcgatg tgagactcag agagtgagga attttctaag gaataaaata 300 gtatcagctt cggtggctga aaatggtaat gctccgacgt cgtttgatta tgatttagta 360 ataattggtg ctggtgttgg tggccatggt gctgcattac atgctgtgga gaagggtctt 420 aaaacagcta tcattgaagg agatgtaatc ggcggaactt gtgtaaacag aggttgtgtt 480 ccttctaaag cacttcttgc agttagcggt aggatgcggg aacttaagaa tgagcatcac 540 ttgaaagctt tgggtataca ggtttctggt gccggctatg acaggcaagc agttgctgat 600 catgctaata atcttgcctc gaaaattcgt aataacttga caaactctct aaaggcactt 660 ggtgtggaca ttttgacagg agtgggtact ataacgggtc ctcaaagggt gaagtatgga 720 aaagttggtt tttctggcag tgaagttacc gcaagggata taattatcgc taccggttct 780 gtcccttttg ttcctagagg cattgaagtt gatgggaaga ctgtgattac cagcgaccat 840 gctctcaaac tggagtttgt acctgaatgg atagctatag tagaagtggt tatattggac 900 ttgaatttag tgatgtgtat accgcacttg gtagtgaggt tacttttatt gaagctctag 960 atcacttatg cctggatttg atccagaaga attagtaagt tagctcaaag attctggtaa 1020 tccaggaaaa ttgattatca cacactggat gtttgccacg aagatcactc ccgcaagaga 1080 tggggagaac agtgtttatg actacctgac gcccgacata agagttaaga acctttggag 1140 gtgatgcacc tcatggcact cgagctcaag ttctatgctg gaattacctg tgacacacag 1200 gtatttctgt cgtgagcgac tcgcacagt 1229 <210> 74 <211> 492 <212> DNA <213> Solanum tuberosum <400> 74 gaagaatgga gataacatgt gcacttgtct gtgaaatctt actagcaatc ttgcttcctc 60 ctcttggtgt ttgccttcgc aatggttgct gcactgtgga gttcttgatt tgcttggtat 120 taaccatatt gggctatgtt cctggaatta tctatgctct ttatgcaatc ctctgtgttg 180 agcgtgaacc acgagtggat cattatgaca gtcttgcttg atctttgttg aatatatcat 240 ttcagattgt gtgcttgttg ttttgttttg cttgtaagtt gtacctatgt tgcttcgact 300 ctacaaaaat gttgcagtcc tcgtgtcgga tcttccaaaa aagcgctatt tttgaaggat 360 ctgacacact cagtcatttt tgaagggtct ttttgactcc aactatggat gtattgtacc 420 ttatgtcact tgttctgaac caactcttgc agtttaagtt aaaaaaaaaa acgaaaaaaa 480 aaaaaaaaaa aa 492 <210> 75 <211> 800 <212> DNA <213> Solanum tuberosum <400> 75 gatcttcctt acattacaaa gtcaataaaa atctgtaaag cttcactgat agccatggaa 60 actttgacgt cttctgccac aactacccct tctctatccg tctttgctcc taaatccaaa 120 catctttctt ctcgtaaatt tgtcaagttt tcagtttcac gcaaaaataa tggaaatgaa 180 cccgatcttc aatctgacgg aaatgaaggc acaagtatcg ttcccatctt caacaatcct 240 actctctcca aggatgcagc tatgggtttg gtccttagtg cagctaatgt aagaggctgg 300 actactggct cgggcatgga ggggccacct gtgcctgctg gttcggattc tgaatccagt 360 actgagcaga tttccacctt cccttggtct ctcttcacta aatctccacg aaggcgtatg 420 cgtgtggcct ttacttgcaa cgtctgtggc caacgaacta ctcgtgccat caatccccat 480 gcctatactg atggcactgt ttttgtccag tgttgtggat gcaatgtatt tcacaagctt 540 gtggataatt tgaacctgtt tcatgagatg aaatgctacg tgagtcccga cttcaatcca 600 aattcagaca atgatattgg cttcaagtat cttgatatgg atgatgacaa cgacgacgtc 660 tttccgcttt tttaaggtct gatagaagga tgtgtaaaaa ttagagtgtt atacattccc 720 ttgcttctat tagcagtttc tctgatcaca tgactgtaaa taacaagtat accctgttta 780 tatgagttat gactgttaat 800 <210> 76 <211> 975 <212> DNA <213> Solanum tuberosum <400> 76 atgggtggcg gtggagcatt cagagcagcg gcgaaggtcg ccggagccgc cgctgccaac 60 agcggattcc gctcggtgac ggtggagcat ccagtctatg cagccgcacg caacgtcccc 120 cgtccggtgt cgatctcagg tatatcgtca acatcggaag atgtcaagtc tgctatggtt 180 acggcttctc acggtggatc atcggatgtg tcgtatgtgc ctaagatggt ttctgatttt 240 gatgactggg agatggccgg cggagaggaa gaaatgatgg ttaatcctgg tgaaccgctg 300 ccacgactcg tatttggtgg agctccttct cttcaggaag ctacagaggc tacttctgat 360 ctcaaagatg ctctggtata tatgtctgga tctgctaatg gatatggggg atcttgtata 420 tccggctcat gctcatcgcc cgtctccaaa gcctgtgttg tcagcgagac tatagtcaca 480 aagtctgtgc cgaaacatgc tgttcaggca tataggattc tgagtgaaat tcctgctgct 540 cagaacgttg ttgcttcaat tgcttgtgac ccaaatgtgt ggaatgcagt actgcaaaat 600 ccagcccttc aagatttcct cgagtcacag agatacagtg agaagtgtgc atcattcccc 660 gactcggatc aagagaaaga tgaatcggtt gctgatactg cttccttttc tgaatcatcc 720 ccatggaagc cgttttctga atctaaggcg gaagagtcta aatccgggaa ctcttttaca 780 agctttttgc agaatgttac acaaacagtg acacaaacag tggttagtat gatggacagc 840 ttgtcagatt tctttaacaa tctctttgga ggcaacaagt tttttgttga tgctgatgga 900 agtgctaaat ttggcgcggg tgggagaaag acaccttgga gcatcttttc atggctttgg 960 cagtcatgtt attag 975 <210> 77 <211> 514 <212> DNA <213> Solanum tuberosum <400> 77 ctactacgtc ggagcagatc caacagattg aagaggtgat ggctccaaaa cagccgaata 60 cagggctatc tgttggccta aacaaaggcc atgttgtgac caagaaggaa ttagctccac 120 gcccttctga cagaaaaggg aaaacaagca aaagaatcca ctttgtgagg agtcttatca 180 gagaagttgc tggatttgct ccatacgaga agaggatcac tgagcttctt aaagttggaa 240 aggacaagcg tgccttgaag gtagccaaga ggaagttggg tactcacaag agggcgaaga 300 ggaagagaga agagatctct agtgttctcc gcaagatgag ggctactgga ggtggtgaaa 360 aggagaagtg aggcctgtat ccaccactat tgatgtttga aggacccagt tagttaattc 420 accagctttt acatttgcaa tgtatttttg ttttcaagaa agacttgtga tcaaaagttc 480 cctgtttatg ttacaaaaaa aaaaaaaaaa aaaa 514 <210> 78 <211> 754 <212> DNA <213> Solanum tuberosum <400> 78 gacacttttg gataaaaaaa atggttaagc taagttgtgc tcctatttta ctagctctgg 60 ttctctgcat cagcctaacc tcagttgcca acgcacagca gtgcggaagg caaaggggcg 120 gagcgttatg tggcaacaac ttatgttgca gccaattcgg gtggtgtgga tcgacacccg 180 aatattgttc acctagccaa ggttgccaga gccagtgcag tggaagtgga ccagatccag 240 gccaaggggg cagcgcgcaa aacgttcgtg caacatatca tatatataac ccgcagaatg 300 ttgggtggga tttgaatgct gttagcgctt actgttctac ttgggatgct aataagcctt 360 acgcctggcg gagtaagtat ggttggactg ctttttgtgg tccagtcgga cctcgtggtc 420 gagactcgtg cggaaagtgc ttaagggtga caaatacacg tacaggagct caaacgacgg 480 tgagaatcgt ggatcaatac agcaatggcg gactagattt ggacattaac gtttttcgac 540 aaatcgatac agatggagta ggaaatcaac aaggacacct tattgtgaac taccaatttg 600 ttaattgtgg tgacaatgtc aatgttcctc tgctttctgt agttgacaaa gaatgagaaa 660 caacgacatg gctctgttta gtctaatggt tgtataaaag tcatctttga tgatttaaaa 720 aaagaataaa agaacgaaaa aaaaaaaaaa aaaa 754 <210> 79 <211> 1253 <212> DNA <213> Solanum tuberosum <400> 79 cagggactca aaaaatcaaa atctacaaaa ttcatagaga aattagctat ggcagcagca 60 acacctctgt tgaaagatga gcttgatatt gtgattccca caataagaaa tcttgatttt 120 ttggagatgt ggagaccctt ttttcagcca taccatctga ttattgttca agatggtgat 180 ccttcaaaga tcattaaggt ccctgaagga tttgattatg agctttataa tcgtaatgac 240 attaacagga ttttgggtcc taaagcttct tgtatctctt ttaaggattc tgcttgtagg 300 tgttttgggt atatggtgtc taagaagaag tatatctaca ccattgatga tgattgcttt 360 gtggccaagg acccgtctgg taaggatatc aatgcacttg agcagcacat caagaacctc 420 ctgtgcccat ccacaccaca cttcttcaac actctgtatg atccatacag agatggtgca 480 gatttcgtcc gtggctaccc tttcagcatg cgtgagggtg ctccaacagc tgtttctcat 540 ggactgtggc tcaacatccc tgactacgat gctcccacac agcttgttaa gcctcatgag 600 aggaacacta gatacgttga tgctgtcatg acgattccaa aaggcacttt gttccccatg 660 tgtggaatga acttggcatt tgaccgtgat ctcattggac ctgcaatgta ctttggtctc 720 atgggtgacg gtcagccaat tggtcgttac gacgatatgt gggccggctg gtgtaccaag 780 gtcatttgtg accatttggg actaggaatc aagactggtc tgccctacat atggcacagc 840 aaagccagca acccattcgt taacctcaaa aaggagtaca acggtatctt ctggcaagag 900 gagatcatac ccttcttcca ggctgcaact ctttctaaag agtgcacaac cgtccaacaa 960 tgctaccttg agctctcaaa gcaagtcaag gaaaactttc cacgatcgat ccctatttca 1020 ccaagctagc agaatgccat ggtcacatgg atcgaagcct tggggatgaa gctcaaccct 1080 actggtgaca cacttgctag ctgtctatcg ccgaatgttc cagcaaagaa cataaaaagt 1140 gaaatgctat tttatagctt tgtctcattc tcccggacat agaacttatg agtaactaga 1200 acctgggagt ttatctgtgt atacaataac tgggttgtat atatatatga ttg 1253 <210> 80 <211> 1288 <212> DNA <213> Solanum tuberosum <400> 80 cagggggaag aacacagtaa agcttgtggg cattcccaat ttggacgaat ttatgaacaa 60 gggcattatc cagaatggga tgaagatcac cctattcatt ttgttgggca ttcagctgga 120 gcacaggttg ttcgagtttt gcaacaaatg ctagctgata aggcgtttaa gggttatgag 180 aacacttgcg aaaactgggt cttaagcgtc acagcattgt ctggagcatt caatgggact 240 acaagaacct actttgatgg aatgaatcct gaagatggga agtccttgag gcctgtgtct 300 ctgcttcagc tttgccgcat tggagtaata atttatgatt ggtttgacat tccttggcta 360 aagaactatt acaactttgg gtttgaccac ttcaacatat cctggagaaa aattggaata 420 tggggtctta ttgattgcct cttgggaaat gctggtccat ttgcttcagg ggattggata 480 cttccagatc ttaccctcca ggggtcgatc aagttgaaca gccacttgca tacgtttcct 540 aacacgtact acttcagcta tgcttccaag cgtaccagca aagtcatggg tataacagtc 600 ccttctggca tactaggtat acatcctctg ctgttcatca gaatcctgca aatgagtcta 660 tggcgccatc ctccagatgt ccctccccca tacaagggct atagggatga ggattggtgg 720 gacaatgatg gtgcactcaa cactatatct atgacacatc ctcgtttccc tgttgagcac 780 ccaagtcaac tggttgttaa agattctgat tgccagcctt ttctacctgg catctggtac 840 tataagattg tcgaaggtga tcacattctt ttcattgtga atcgtgaaag agctggagtt 900 caatttgatt tgatctatga cagcatattt gagcgttgca ggaagcatgc cttcagaaag 960 aatccgacaa tgccaaacca tataagttaa tagttagcaa agatgttgct cttactaatg 1020 cgcgctacaa acactttaga gagtagaggt taaagaaaag ggaatctgtg atatccaaat 1080 actcttctgg cgcttcttgc ttcttttttc atcagacaaa aaaactatct tccctttgaa 1140 atgtaaatgt gaataggctt gcagaggaaa aactatttag tgtctaggct tgcagaggaa 1200 aaatgtgaat tgaggagaca tgacacactg gtctcttgta ttattgaaaa cataaagata 1260 gattttcacc aaaaaaaaaa aaaaaaaa 1288 <210> 81 <211> 683 <212> DNA <213> Solanum tuberosum <400> 81 cagggggaga gaaaactagt tatggagata gcggatacgg agagaaacct agttatggtg 60 ggggagatga caacaaatat ggtgagaaaa ctagttatgg taatgaggaa ggtggctatg 120 gtggaggagt tggtgagact actaattatg aagagaatga aagtgaaacc aaaacttctg 180 aggattacaa ggaagagaag aaacaccaca agcatcttga agaaattggg ggactcggag 240 ctgttgctgc tggtgccttt gctttgcatg agaagcacaa ggcagagaaa gacccagaga 300 atgcacacaa gcacaagata gaggaaggaa tagcagctgc tgctgcaatt ggtgctggtg 360 gatttgcatt ccatgaacat catgagaaga aggaagcaaa ggaagaagag gaggaagctg 420 agggaaagaa gaagcatcat ttcttcaatt aattctccta tcataatata tttacctctc 480 tttgttgtgt gctgatcaat ctatccttta attaatactc agcacctaaa taatttgtat 540 gtgtgttaca gtactcttgt gtttgatgta agcgttactt ggtgaatttg gcgttttaag 600 tcacctacat gttgtagtac tttgcatgag tgtgaataat actattacgt atattgtttt 660 tgccaaaaaa aaaaaaaaaa aaa 683 <210> 82 <211> 1760 <212> DNA <213> Solanum tuberosum <400> 82 cagggggtta gcatatagta cattattaac ctactgctat tcttcttctt gtactctgtc 60 aaacaagcgt aagactgaga atacagagga gaccaaaaat ggaagctgaa tatggaaaat 120 catcctctgt aaaaactcca ttgatagatg accgagaaaa acagagtatt cttgatgaaa 180 caagaaaaaa tggtggtggg tttagcaagg aagagattgt tgatgaagtg aagaggcaag 240 tatggctagc aggaccctta gtgagcgtta accttttgca gttttcttta cagcttatat 300 ccattatgtt tgtgggtcat cttggaaaat tgcccctttc tggtgcttcc atggctaatt 360 ccttcacttc tgttactggt atcagccttc tgatggggat gtcaagtgca ttggacacat 420 tttgtggaca gtcttatgga gcaaaacagt atcatatgct tggcattcac atgcagagag 480 ctatgattgt actttcactt gtaagcgtac ctcttgcagt aatctgggca aacactggcc 540 ctattctcaa ggttttaggc caagatccta gtatatctga tgaagctggt caatatgcct 600 tgtattttat ccccggtgtt tttgcttacg gcttgctcca atgtgttgtg agatttttac 660 aaacacaaag tattatcatc cctatggtgt tatgtgctgg agttacaacc ttaatccacg 720 ttgttgtctg ttggattttg gtcttcaaga ctggacttgg tgtcaaagga gctgctctag 780 caaattcaat ctcttactgg cttaacttct tgtttttggt tctttatatc aagttttctc 840 cttcatgtgc aaaaacttgg acaggactgt caaaagaagc tttgaaggat atgcttactt 900 tccttagact tgcggttccc tcggccatca tggtctgctt agaaatgtgg tcgtttgagc 960 taatggttct gctttctggc ctccttccaa atccacagtt ggaaacgtca gtgctctcaa 1020 tctgtcttaa tacagccgca acagtttgga tgatcccttt tgggctgagt ggtgctgtga 1080 gtacacgggt ctcaaatgaa ctaggagcag ctcatcctca acttgcacgt ttggcgctgt 1140 atgttgtctt agcggtggct gttattgagg ggcttgtgtt gggattggtc ttgatattga 1200 tacgtaaagt atggggattt gcttacagca acgaaactga agttgttaaa tacatagcca 1260 tcatgatgcc acttcttgca acatcaaatt tcttggatgg ccttcaatgt gttctctcag 1320 gtgctgtaag aggatttgga tggcagaaaa ttggggcaat tattaacctc ggatcctatt 1380 atttggtggg aattccttgt gctgtcttgt tggcctttta tcttcacatt ggtgggaagg 1440 gattatggtt gggtattatt tgtgcacttc tagtacaagt gttgtctctt cttttcatta 1500 ccatacgcac caactgggaa caagaagcaa agaaggctca agagagagtg gaagaaacaa 1560 cattacccgc ggagatagtg tactgaaaat tgaagtaatt gatcaaaaga ttttgacctg 1620 tttgacgtat ggtttaacgt cttaagaact ctaatacagt tttgtggctt tgatttgcgg 1680 catattgtgg actaaatatc aatggcttac tatcttaata taaatttaag tttcctctcc 1740 aaaaaaaaaa aaaaaaaaaa 1760 <210> 83 <211> 842 <212> DNA <213> Solanum tuberosum <400> 83 caggggctct ctctctctcc cacttttcag gaatcatctc ttagattctt caatggcctt 60 gagagctgga ccggttttct gtgaacttaa accacaaact cagaagcctg aaccggccgg 120 ttttatgccg gttcagaatt tgagagtgtc agaaccgggg caggagaatg ggaaaatcat 180 gttgcagcct agattgacaa ctttaagatc attcgggtcg gatcctgtcg gagtgatcaa 240 gacgaagaac ggaggtttta atggcgaaga tgatgaggtt tcgccttttt tcgcaacgct 300 atcggagtat attgaaagct ctaagaagag tcatgatttt gaaattatat ccggtcgact 360 tgccatgatt gtgtttgcag caacagtgtc tatggaagtt gtgactggaa attcagtatt 420 cagaaagact gattttcaag gaatagctga agctgctggg gtttgtattg gtgccgcggc 480 ttgtgctgct ctttttgctt ggtcttccag ttctcgcacc cgtgttggcc gaattttcac 540 ccttggttgc aacaccttca tcgattcctt catcgatcaa atcgtcgatg gcttgtttta 600 tgacatcaac gattggactg atgatatcta gacctcaaat attggaaaaa gggaagtgat 660 atacttaagt agttgagttt atccaatgtt tcaagtacaa tgtgtttcat tagaccttgg 720 ttagttaata tatatatatg ttgtaaatag atatcatata gttaaaagat attattatta 780 ttataaagaa agttatagta caataatata taaaccatca aaagaaaaaa aaaaaaaaaa 840 aa 842 <210> 84 <211> 411 <212> DNA <213> Solanum tuberosum <400> 84 atggatatct gggtgcaatc tgttggggga aagaaaaaag gaagagttaa aggtcttgga 60 tccctgggtc gatctgtaaa gtcatcgtct aaacaatcaa catcagcttt atcgagagaa 120 attgatgaaa tgattaaagc tcaggtaaat gcttccaatg ccgacttata tgctcaactg 180 cagaaagagc gtcacaaaaa taagaggatg aggagggaat tggacttatt aaagaaacat 240 gtatacaacg catcatcttc aaatgagcaa tcatctcaag aagacaatca agcttatgag 300 aatgaaagtg gtgatgactc tgatagcgtg aatgatagtg gttctgtcca tgataacgtg 360 aatgacagtg gttctgacca ttatagcgca tcatcttcaa atggggatta a 411 <210> 85 <211> 884 <212> DNA <213> Solanum tuberosum <400> 85 cagggatctc tatgtaaata tccactccct ctctcctcac catctctccc actatccaca 60 cactctctct ctgtctctct cttctctttc catggctgct gtactagaaa ctattacagt 120 cccacgcgca tcggcgctgc cgtcgtcgtc gcttgctccg gtcgccggtt attccttctc 180 cggtcctcgt tcctctgtta gattctctca atccagcggc cttaagatcc agccaattcg 240 ctcatccgta tccactagct catgctctaa aatcattccc cgaggaggac gagtcgtttg 300 cgaagctcag aatgctgccg tcgaagtggc tgctgttagt gataaaacat ggaagacact 360 tgtagtcgag tctactgtac ctgtactggt tgaattttgg gctccgtggt gtggtccttg 420 ccgaatgatc caccctgtca ttgatgaact agcaaaggaa tatgttggca agcttacatg 480 cttgaagctg aacacagatg aaagtccttc cattgcaacc gaatttggaa tccgaagcat 540 cccaactgtg atgatattca agaatggaga gaagaaagat gcaatcattg gtgcagttcc 600 taaatcaaca cttaccacct ccatagaaaa attcttgtaa aatgttgcat atgtagatat 660 attatctggg aatttactca tatattccct tttcagcctc cagtgcggca atcgtttaag 720 taaaccagta atgctcctgt atagttgggt tgcattgtgt ttccccttac ttaaacgtta 780 cgttgattgt ttctcatgtt tagaaattca cttttactta taaatgcttt taaacaaagc 840 ataatcctac ttcatgcttt gaatgaaaaa aaaaaaaaaa aaaa 884 <210> 86 <211> 667 <212> DNA <213> Solanum tuberosum <400> 86 gacccaacac tcaaaacaaa tacatttgta gtattatatc atcctctact atttctatcg 60 atctttactc aatttttgta acaatggaaa tgtttggcaa aattgcatgc tttgtgcttt 120 tgtgcatggt ggtggttgca ccccgtgcag aggcactgag ctgcggcgag gttacatctg 180 gcttggctcc ctgcctccct tatcttcaag ggcgcggtcc tattggaggg tgttgtggtg 240 gtgttaaggg tctgttgggt gcagccaaga ccccagaaga ccggaagaca gcatgcactt 300 gcctgaaatc ggcagctaat tctattaagg gcattgatac gggaaaagcc gctggtctcc 360 ctggagtttg tggagtcagc attccttaca agatcagccc ttccactgat tgctccaagg 420 tccagtaagg ttgatgaaat taaagctaag tctatatata gcttgcggag atgaagaata 480 agatggatat atatcgatcg agtttgatcc atcattatat atgttgtctc ttttcttttg 540 tatttgtgtt tgttggagta cttatatatt gtcgagtctt gtaatgaaca ttagtggttg 600 cttgtgttac gacccaatct tcatatagta atacatgata ttttactaaa aaaaaaaaaa 660 aaaaaaa 667 <210> 87 <211> 1018 <212> DNA <213> Solanum tuberosum <400> 87 caggggagaa ccccaattcc aaagctctca tcttcatctg ctcactgtca ctgttgcctc 60 actgccggcg gtggagtctc cgattaagta ctgatggtat ttcttagctg gacgcgtcca 120 tctcctgacg agcagaaagc atgtatcaac aagtctggtt cctttaacta tgataataga 180 tttagaggag ctactgataa acctgctcct ctacttaagc aagacaggga gcttgcaaag 240 gatggtttct ctgttaattg tgctcgtgta ttagtcggtt caggccgtga gacttttgag 300 aagggaaaag ctgcccttca aaattggagg cattttggat tgaattgggc gtttgttgat 360 ccaaaagccc caattcaaag tgggacaaag ttctgtgttt gcgtgaagga gttcttcccg 420 tggcttatga tgcctcttca ggtggtttat gtcaccgaga acaagaactc gaaaatgggt 480 gcttcattta gttttggcag tggaactctc caaggacatt tgctggctgg tgaagaacgt 540 ttttcaatag cattggacga aaatgatcta gtttggtacg agatactttc cttctcgaag 600 cctgcacatc tgctgtcact ctttggatac ccttatgttc ttctcaggca aaaatacttt 660 gctcatcatt caggtattgc ggtgaagaaa catttatctg cttaaaacat gtggggctat 720 aactgtgtgt aaaaaaaatg ctcttcattg tagacaggaa atcttagttg taaaagattc 780 ccatccatag ttatattggg tcttgatttc atgttttttt ctttgcatta tatgcacgac 840 ctagttacta ataatgcaat tctagtcctt gttaaagaaa atgtaattgt agtccaatag 900 ctggataatc agtgtgatat aattaagtga aaagcataga agaaaaacta tgcataagac 960 ccaatagcga gaatcatttc aggaaagggt cattcattct acgaaaaaaa aaaaaaaa 1018 <210> 88 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer 1 <400> 88 ttgataccac tgcttagggc gagcttaata ttccct 36 <210> 89 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer 2 <400> 89 tctcatcgta ccccgctcct cggtctcgat tctacg 36 <210> 90 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> sequencing primer <400> 90 aatatacctc tatactttaa cgtc 24

Claims (8)

An expression vector for producing a cold stress resistant transformant comprising a dry stress resistant gene, wherein said gene is a gene selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 87. The method according to claim 1,
The gene is potato ( Solanum tuberosum ) derived expression vector. Dry stress resistant transformant prepared by introducing the expression vector of claim 1 into a host cell. The method of claim 3,
The transformant is a transformant plant. The method of claim 4,
The plant is potato, onion, eggplant, tobacco, carrot, cucumber, olive, sweet potato, cabbage, cabbage, radish, watermelon, melon, cucumber, pumpkin, strawberry, soybean, green beans, green beans, lettuce, pepper, tomato, broccoli, sunflower , Gat, turf, Arabidopsis, rapeseed, and gat transformants. Preparing an expression vector of claim 1;
Preparing a transgenic plant cell by introducing the expression vector into a host plant cell; And
Method of producing a plant having a dry stress resistance comprising the step of recultivating the transformed plant cells in tissue culture. The method of claim 6,
The introduction of the expression vector into the host plant cell is Agrobacterium-mediated method, gene gun, silicon carbide whisker method, sonication, electroporation and PEG precipitation method is selected from the group consisting of. A method of increasing the dry stress resistance of a plant comprising the step of introducing the expression vector of the plant into the plant.

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