KR20140023193A - Skin whitening and anti-oxidative composition containing boesenbergia rotunda extract - Google Patents

Abstract

The present invention relates to a skin whitening and anti-oxidative composition containing a Boesenbergia rotunda extract as an effective component. The Boesenbergia rotunda extract of the present invention inhibits the generation of melanin, has an anti-oxidative action, and has no cytotoxicity to be used as a skin whitening and anti-oxidative composition.

Description

Skin Whitening and Anti-oxidative Composition Containing Boesenbergia rotunda Extract

The invention beam senbeol Jia rotunda (Boesenbergia rotunda ) relates to a composition for skin whitening and antioxidant comprising the extract as an active ingredient.

Many factors are involved in determining the skin color of humans, including the activity of melanocytes that make melanin, the distribution of blood vessels, the thickness of the skin, and the presence or absence of pigments in the body, such as carotenoids and bilirubin. It is important. In particular, the most important factor is the black pigment called melanin (melanin) produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of this melanin pigment is influenced by genetic factors, physiological factors related to hormone secretion and stress, and environmental factors such as ultraviolet irradiation.

This melanin is synthesized in melanocytes present in the basal layer of the skin, and the most important enzyme in this pathway is tyrosinase. The resulting melanin is transferred to the surrounding keratinocytes in the melanosome state and removed by keratinization. However, if this process is not progressed smoothly due to hormones or external environment, pigmentation such as blemishes may occur.

In particular, ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, or the like in order to treat or alleviate excessive melanin pigmentation caused by skin pigment abnormality symptoms such as blemishes, freckles and pigmentation, and UV exposure, etc. Derivatives and substances having tyrosinase inhibitory activity have been used in cosmetics and pharmaceuticals, but their use is limited due to insufficient whitening effects, stability problems on the skin, formulation and stability problems when formulating cosmetics, and the like.

Meanwhile, in addition to these existing whitening efficacy substances, research is being conducted to find whitening active ingredients in natural products. Among them, Sangbaekpi (Japanese Patent Publication No. 55-44375, So64-26507, So64-83009, Pyeong1- 1) 25687, Hei 5-139950, Korean Laid-Open Patent Nos. 1992-002109, 1997-021273) and Licorice (Japanese Laid-Open Patent Nos. 60-214721, Small 63-23809, Small 64-63506, Flat 1-49706, Korean Publication Patent No. 1992-002109, No. 1997-025601) and many plant extracts have been found to act on tyrosinase to inhibit melanin production (Korean Patent Publication No. 2011-0220069, 2011-0120030, Japanese Patent Application Laid-Open No. 2007-22253, 2010-240422), and these also have many problems in using above the effective concentration in cosmetics and pharmaceuticals in terms of safety, stability and discoloration, and do not produce satisfactory effects. It is true.

Boesenbergia rotunda is a plant belonging to the family Ginger. Bosenenbergia rotunda extract has anti-inflammatory activity (Tuchinda et al., Phytochemistry , 59: 169, 2002), antimicrobial activity (Rukayadi et al., Bio . Pharm . Bull . , 33: 1489, 2010) and anti-ulcer activity (Abdelwahab et al., J. Ethnopharm . , 137 (2): 963, 2011), but are known to be effective in skin whitening containing Bosenbulgia Rotunda extract. There is no disclosure of the composition.

Accordingly, the present inventors have made diligent efforts to develop natural extracts having skin whitening and antioxidant effects among various natural extracts that have not been known so far, and confirmed that Bosenbulgia Rotunda extract has skin whitening and antioxidant effects, and completed the present invention. Was done.

An object of the present invention is to provide a skin whitening or antioxidant composition comprising Bosenbulgia Rotunda extract excellent in skin whitening and antioxidant effect.

In order to achieve the above object, the present invention provides a composition for skin whitening comprising Boesenbergia rotunda extract as an active ingredient.

The invention further beam senbeol Jia rotunda (Boesenbergia rotunda ) provides an antioxidant composition comprising the extract as an active ingredient.

The present invention also provides beams senbeol Jia rotunda (Boesenbergia rotunda ) provides a cosmetic composition having skin whitening or antioxidant ability, including the extract as an active ingredient.

Beams of the present invention senbeol Jia rotunda (Boesenbergia rotunda ) extract inhibits melanin production, has antioxidant activity and no cytotoxicity, so it can be usefully used as skin whitening and antioxidant composition.

Figure 1 is a graph showing the melanin production when treated with Bosenbulgia Rotunda extract.
Figure 2 is a graph showing cell viability upon treatment with Bosenbulgia Rotunda extract.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art.

In one aspect of the present invention, the present invention relates to a composition for skin whitening comprising Bosenbulgia Rotunda extract as an active ingredient.

In the present invention, the Bosenbulzia rotunda extract may be characterized in that it is extracted by using Bosenbulgia Rotunda selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 3 carbon atoms, and a mixed solvent thereof. In addition, the amount of the extraction solvent may be 2 to 20 times the dry weight of the herbal medicine.

In the present invention, Bosenbulzia rotunda extract may be characterized in that it is extracted using a method selected from hot water extraction, heat extraction, reflux cooling extraction, ultrasonic extraction and immersion extraction.

The extraction method is preferably a hot water extraction method of 30 ℃ ~ 70 ℃, more preferably 40 ℃ ~ 60 ℃, more preferably maintaining at 40 ℃ is most effective in the prevention of rot, discoloration and odor, The extraction time is 5 to 30 hours, preferably 24 hours.

After obtaining the Bosenbulgia Rotunda extract, the solids are removed using a filter paper, and the suspension is centrifuged to filter the supernatant under reduced pressure, and the filtered extract is heated to dryness, reduced pressure drying, spray drying and freezing. It can be characterized by using a method such as drying.

In one embodiment of the present invention, finely pulverized 10 g of dried Bosenbulgia Rotunda root, and then, 200 ml of water was added, and the extract obtained by hot water extraction in a 40 ° C. water bath for 24 hours was cooled at room temperature, filtered with a filter paper, and filtered. Obtained. The filtrate obtained was concentrated under reduced pressure and lyophilization to obtain 1.07 g of Bosenbulzia rotunda extract.

In order to assay the skin whitening effect of Bosenbulzia rotunda extract of the present invention, a test for measuring melanin biosynthesis inhibition effect in B16F1 cells was performed. As a result, in the case of arbutin, known as a melanogenesis inhibitor, melanin production was 67.5% at the concentration of 200 µg / ml, and bosenbulgia rotunda extract was 66.6% and 1mg at 0.6mg / ml. 52.8% melanin production in / ml showed a similar or superior melanin inhibitory effect of arbutin, and the survival rate test in B16F1 cells to confirm the cytotoxicity of Bosenbulgia extract, Compared with the untreated treatment, the extracts of Sverggia rotunda showed 90.8% survival at 0.6mg / ml and 76.4% at 1mg / ml.

In another aspect of the present invention, the present invention relates to an antioxidant composition comprising Bosenbulzia rotunda extract as an active ingredient.

In the present invention, the Bosenbulzia rotunda extract may be characterized in that it is extracted by using Bosenbulgia Rotunda selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 3 carbon atoms, and a mixed solvent thereof. In addition, the amount of the extraction solvent may be 2 to 20 times the dry weight of the herbal medicine.

In the present invention, Bosenbulzia rotunda extract may be characterized in that it is extracted using a method selected from hot water extraction, heat extraction, reflux cooling extraction, ultrasonic extraction and immersion extraction.

The extraction method is preferably a hot water extraction method of 30 ℃ ~ 70 ℃, more preferably 40 ℃ ~ 60 ℃, more preferably maintaining at 40 ℃ is most effective in the prevention of decay, discoloration and odor, The extraction time is 5 to 30 hours, preferably 24 hours.

After obtaining the Bosenbulgia Rotunda extract, the solids are removed using a filter paper, and the suspension is centrifuged to filter the supernatant under reduced pressure, and the filtered extract is heated to dryness, reduced pressure drying, spray drying and freezing. It can be characterized by using a method such as drying.

In order to measure the antioxidant effect of the Bosenbulgia Rotunda extract of the present invention, the phenol content having an antioxidant effect was measured in the Bosenbulgia Rotunda extract, and it was confirmed that the total phenol content was 29.8 mg / g. In addition, the antioxidant activity by the DPPH method generally used is used as a measurement result of measuring the antioxidant activity of a beam senbeol Jia rotunda extract, beam senbeol IC ₅₀ of Jia rotunda extract The value of 21.2 mg / mL was confirmed that the antioxidant effect of Bosenbulgia Rotunda extract.

The skin whitening and antioxidant composition preferably contains 0.005 to 50% by weight of Bosenbulzia rotunda extract based on the total weight, but above or below the range according to the use purpose in the range of whitening and antioxidant effects and no toxicity appears It can also be used as.

The skin whitening and antioxidant composition may contain one or more active ingredients exhibiting the same or similar function in addition to Bosenbulzia rotunda extract.

In another aspect of the present invention, the present invention relates to a cosmetic composition having a skin whitening or antioxidant capacity comprising the Bosenbulgia Rotunda extract as an active ingredient.

Cosmetics prepared with the skin whitening and antioxidant cosmetic composition containing the Bosenbulgia Rotunda extract of the present invention as an active ingredient may be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of emulsified form include nutrition lotion, cream, essence, etc., and cosmetics of solubilized form have softening longevity.

As a formulation of the skin whitening and antioxidant cosmetic composition containing Bosenbulzia rotunda extract of the present invention as an active ingredient, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, Formulated with nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder, eye shadow It may be applied to a conventional cosmetic preparation method. Relief can be applied in various forms such as liquid, cream, paste and solid, and may include various conventional auxiliaries and carriers suitable for each of these formulations and well known in the art.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these examples are for illustrative purposes only and that the scope of the present invention is not construed as being limited by these examples.

Example  One: Bossenbulgia Rotunda  Preparation of extract

The Bossenbulzia rotunda of the present example used what was collected from Malaysia.

The collected plants were washed with water to remove foreign substances, dried, and finely pulverized 10 g of Rosenda Rosunda root, and 200 ml of water was added thereto and warmed in a 40 ° C. water bath for 24 hours. The obtained extract was cooled to room temperature and filtered through a filter paper to obtain a filtrate. The filtrate was then depressurized and concentrated to prepare 1.07 g of Bossenbulzia rotunda extract.

Test Example  One: Bossenbulgia Rotunda  Whitening effect measurement

1-1: B16F1O Melanoma Cell Culture

B16F10 cells (ATCC No. CRL-6475) are used as a product from the American Type Cell Collection (ATCC), and the B16F10 melanoma cells are generally used for the validation of whitening substances as cell lines producing melanin. The medium for culturing the cells was Dulbecco's Modified Eagle's Medium (DMEM) containing 10% FBS, 1% penicillin / streptomycin, and the cells were cultured in an incubator at 37 ° C., 5% CO ₂ . B16F10 cells were passaged when the 100 mm Petri dishes were about 90% full.

1-2: Determination of melanin inhibitory effect by measuring absorbance

In order to measure the whitening effect of Bosenbulgia Rotunda extract, the change of melanin produced by treatment of Bosenbulgia Rotunda extract or arbutin on B16F10 cells was measured. The cells were seeded in 6-well plates, treated with Bosenbulzia rotunda extract obtained in Example 1 or arbutin commercially available at a constant concentration for 3 days, followed by incubation with trypsin-EDTA. Was recovered. 1N NaOH containing 10% DMSO (dimethylsulfoxide) was added to the recovered cells to react at 80 ° C. for 1 hour to allow melanin to be sufficiently dissolved, and then absorbance was measured at a wavelength of 405 nm using an ELISA reader. . Melanin production was calculated using Equation 1 below.

As a result, in the case of arbutin, known as a melanogenesis inhibitor, melanin production was 67.5% at the concentration of 200 µg / ml, and bosenbulgia rotunda extract was 66.6% and 1mg at 0.6mg / ml. It was confirmed that melanin production inhibitory effect similar or superior to arbutin at 52.8% at / ml (Fig. 1, Table 1).

[Equation 1]

Melanogenicity (%) = treatment absorbance / no treatment absorbance × 100

Inhibitory Effect of Bosenbulzia Rotunda Extract on Melanin Production Name of sample Melanin Production (%) Non-treatment 100 Arbutin (200 ug / ml) 67.5 Bossenbulgia Rotunda Extract (0.6mg / ml) 66.6 Bossenbulgia Rotunda Extract (1mg / ml) 52.8

Test Example  2: Bossenbulgia Rotunda  Toxicity Test of Extracts

To determine whether the extract of Bosenbulzia rotunda can be used as a whitening cosmetic composition, a cytotoxicity experiment was conducted. B16F10 cells were inoculated into 96 well plates, and Bosenbulgia Rotunda extract obtained in Example 1 was treated with 0.2 mg / ml, 0.4 mg / ml, 0.6 mg / ml and 1 mg / ml, respectively, at 37 ° C. for 10 days. Incubated in an incubator of% CO ₂ . After incubation, the medium was removed, and 100 μl of a solution of MTT (3- (4,5-dimethythiazol-2-yl) -2,5-diphenyltetrazoliu, bromide) was added thereto and reacted at 37 ° C. for 4 hours. After the reaction, the solution was removed, and 100 μl of DMSO was added to dissolve the stained cells, and then the absorbance was measured at a wavelength of 540 nm using an ELISA reader. Cell viability was calculated using Equation 2 below.

As a result, when compared to the untreated treatment with Bosenbulzia rotunda extract, 90.8% at 0.6mg / ㎖, 76.4% at 1mg / ㎖ showed a high melanin production inhibitory effect without toxicity (Fig. 2, Table 2).

&Quot; (2) "

Cell survival rate (%) = (treatment absorbance / non-treatment absorbance) × 100

Cell Viability of Bosenbulzia Rotunda Extract Name of sample Cell viability (%) Non-treatment 100 Arbutin (200 ug / ml) 108.9 Bossenbulgia Rotunda Extract (0.6mg / ml) 90.8 Bossenbulgia Rotunda Extract (1mg / ml) 76.4

Test Example  3: Bossenbulgia Rotunda  Determination of Total Phenolic Content and Antioxidant Effect of Extracts

The total phenolic content of Bosenbulzia rotunda extract extracted in Example 1 was measured by Folin-Denis method (G. Miliauskas et. al . , 2004), Phenolic substances are known to have a function of binding to biopolymers to exhibit an antioxidant effect. After mixing the sample 1N Folin-ciocalteu solution in 1 ml of Culcuma islandia extract, and left for 5 minutes, 7.5% Na ₂ CO ₃ was added and the absorbance was measured at 765 nm. The content was calculated using a standard curve made of gallic acid, and the total phenol content was found to be 29.8 mg / g.

In addition, the antioxidant activity of Bosenbulzia rotunda extract was measured using DPPH method, which is commonly used for measuring antioxidant activity (Chang et al . , 2002). 50 μl of Bosenbulzia rotunda extract and 950 μl of 0.004% DPPH solution were mixed and reacted at 37 ° C. for 10 minutes, and the absorbance was measured at an wavelength of 515 nm using an ELISA reader. As a result, IC ₅₀ of Bosenbulzia rotunda extract The value was confirmed to be 21.2 mg / mL.

Formulation example  One: Bossenbulgia Rotunda  Containing extract as an active ingredient Of whitening cosmetics  Produce

1-1: Manufacturing Flexible Softener

Formulation example of the flexible lotion containing Bosenbulzia rotunda extract obtained in Example 1 as an active ingredient was prepared as shown in Table 3.

Constituents of Softened Longevity Containing Bosenbulgia Rotunda Extract Raw material Content (parts by weight) Bosenbulgia Rotunda Extract 0.002 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 Hydrogenated castor oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

1-2: Preparation of Nutritional Cream

Formulation example of the nutrition cream containing Bosenbulzia rotunda extract purified in Example 1 was prepared as shown in Table 4.

Constituents of Nutritional Cream Containing Bosenbulgia Rotunda Extract Raw material Content (parts by weight) Bosenbulgia Rotunda Extract 0.002 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlyric / capric triglyceride 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Preservative Suitable amount Flavoring agent Suitable amount Purified water Balance

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereby. something to do. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (7)

Senbeol Jia Bo Rotunda (Boesenbergia rotunda ) Skin whitening composition comprising the extract as an active ingredient.
The method of claim 1, wherein the Bosenbulgia Rotunda extract is extracted by using Bosenbulgia Rotunda selected from the group consisting of water, an anhydrous or hydrous lower alcohol having 1 to 3 carbon atoms, and a mixed solvent thereof as a solvent. Composition.
The composition of claim 1, wherein the Bosenbulzia rotunda extract is extracted by a method selected from hot water extraction, heat extraction, reflux cooling extraction, ultrasonic extraction, and immersion extraction.
Senbeol Jia Bo Rotunda (Boesenbergia rotunda ) Antioxidant composition comprising the extract as an active ingredient.
The method of claim 4, wherein the Bosenbulgia Rotunda extract is extracted by using Bosenbulgia Rotunda selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 3 carbon atoms, and a mixed solvent thereof as a solvent. Composition.
The composition of claim 4, wherein the Bosenbulgia Rotunda extract is extracted by a method selected from hot water extraction, heat extraction, reflux cooling extraction, ultrasonic extraction, and immersion extraction.
Senbeol Jia Bo Rotunda (Boesenbergia rotunda ) A cosmetic composition having skin whitening or antioxidant ability, comprising an extract as an active ingredient.

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