KR20140004459A - Manufacturing method of extract from bulnesia sarmienti containing catechins, extract from bulnesia sarmienti manufactured by the same, high catechins granular tea containing the same and food composition for removing active oxygen, obesity control, body fat reduction containing the same - Google Patents

Abstract

The present invention relates to a method for manufacturing Bulnesia sarmienti extract containing catechins, the Bulnesia sarmienti extract containing catechins manufactured by the same method, high catechins granular tea containing the same extract, and a food composition containing the same for removing the active oxygen in the body, controlling the obesity, and reducing body fat. The method for manufacturing the Bulnesia sarmienti extract comprises: a step for adding 35-50 mL of ethanol per 1 g of the Bulnesia sarmienti and extracting at 88-90°C for 1-2 hours(step 1); a step for filtering the extract(step 2); and a step for drying the filtered extract for 4-5 hours(step 3). The high catechins granular tea containing the Bulnesia sarmienti extract containing catechins, dietary fiber, Inonotus obliquus extract powder, extracts from fermented embryo bud of rice and soybean, and barley extract powder has effects in removing the active oxygen in the body, controlling the obesity, and reducing the body fat and anti-viral effect. [Reference numerals] (AA) Bulnesia; (BB) Regular green tea

Description

Method for preparing blancia salmity extract containing catechin, blancia salmity extract containing catechin prepared by this, high concentration of catechin granules comprising the same and free radicals in body including obesity, obesity control and body fat improvement Manufacturing Method of Extract from Bulnesia Sarmienti containing Catechins, Extract from Bulnesia Sarmienti manufactured by the same, High Catechins Granular Tea containing the same and Food Composition for removing active oxygen, obesity control, body fat reduction containing the same}

The present invention provides a method for producing a catechin-containing blancia salmity extract, blancia salmity extract containing catechin, high concentration of catechin granules comprising the same and the removal of free radicals in the body containing the same, obesity control and The present invention relates to a food composition for improving body fat, and more particularly, to bleeding free radicals, controlling obesity, and improving body fat, including Blencia salmity extract containing catechin, dietary fiber, embryonic soybean fermentation extract, and barley extract powder. Method for the preparation of the catechin-containing blancia salmity extract, the catechin-containing blancia salmity extract, high concentration catechin granules comprising the same and the removal of free radicals in the body containing the same, obesity control and It relates to a food composition for improving body fat.

With the introduction of Western diet, intake of calories through animal foods and processed foods is increasing, and obesity, diabetes, hyper-cooperative diseases, etc. are increasing due to adult diseases. In particular, obesity due to excessive intake of animal fats is considered to be an important cause of the development of chronic adult diseases.

Bulnesia Sarmienti grows only in the form of nocturnal in the deep outcrops of the Americas, and has been widely used by the Aboriginal people as a mysterious herb for healing diseases.

Currently, Guaiacwood Oil extracted from Blencia Salmonti is commercialized and sold in the United States and Europe, which are registered in US FDA Food Code 21CFR, PART 172, Sec.172.510. It was confirmed that stability was obtained in single dose toxicity test and residual pesticide test of BioScreen testing Service.

In relation to the Blensia Salmity, the Korean Patent Registration No. 10-0890945 (20019.03.27.) "Blensia Salmity Extract containing beverages and a method for preparing the same" A beverage containing the active ingredient and a method of manufacturing the same are disclosed, and Korean Patent Registration No. 10-0955726 (2010.05.03.) "Blensia Salmonity Extract having a blood lipid improvement, body fat reduction or blood circulation improvement effect" It is disclosed that the extract of Elencia salmity and mixed extracts added alone or in combination with Salvia soybean or lettuce extract have the effect of body fat reduction, blood lipid improvement or blood circulation improvement, and Korean Patent Publication No. 10-2011-0085433 ( 2011.07.27.) "Blencia Salmity Extract-Containing Hangover Relief Composition" discloses a method for producing a hangover relief composition for reducing alcohol and acetaldehyde concentrations in blood. There.

In addition, the antioxidant and anticancer effects of diabetic extracts of Blencia Salmonti, improvement of diabetic complications, stability of single oral dose toxicity, anticancer effect on lung cancer cell H460, activated LPS RAW264.7 intracellular Blencia The anti-inflammatory response, lipid lipid-improving effect and blood circulation-improving effect have been found. However, there is no patent for high quality catechin granular tea using catechin extracted from Blencia Salmity.

Blensia Salmonti, which has been eaten among the natives of the United States for a long time, contains various functional substances such as caffeine, catechin, theanine, and amino acids. Journal of the Korean Society of Physiology and Pathology, Vol. 19, No. 4, 2005), Anticancer and Antioxidant Effects (Cho Dae-Hyun, et al. , Body Fat Reduction Effect (Park Chang-Ho et al., Effects of Blensia Salmonity Single Extract and Complex Extract on Serum Lipids and Body Fat in Rats Fed High Fat Diet, Journal of the Korean Society of Food Science and Technology, Vol. 40, No. 4, p. 449 ~ 454, 2008, Vol. 40 No. 4, p 455 ~ 459), Human Non-toxic Test (Park, Chang-Ho et al., Outlined by single oral administration to rats to ensure the safety of complex extracts containing blancia extract Are said to be effective for Research, Korea Experimental Animal Association, in 24 2008 (1), p 83 ~ 86) of the tetramer and toxic, are also being studied for its efficacy is still done.

Most of the ingredients in Blensia Salmonti are composed of catechins, and recent studies show that catechins play an important role in physiological activity of our bodies. (A review of latest reserch findings on the healthpromotion properties of tea, Christiane J, Dufresne, Edward R. Farnworth, J. Nutr. Bichem, Dec. 2001, 404--421)

In addition, the content of catechin in the existing green tea is generally different according to the cultivation environment and time of the tea growing region, the soil, etc., but the catechin content is on average 10-to-total dry weight of tea leaves. The chemical composition of wild green tea of Jirisan, Shin Mi-kyung, Korean Tea Culture Association, March-April 2000, 30-33. It has a catechin content ratio of%, which is more than 5 times the content of catechins compared to conventional green tea.

However, there are no reports on the optimal conditions for the extraction of catechins from Blensia Salmity.

In view of the above, the inventors of the present invention showed that the optimal extraction conditions of catechin in the Blensia salmity were 30-50 mL / g solvent ratio, extraction temperature 88-90 ° C., extraction time 1-2 hours, and drying time 4-5 hours. The catechin extract tea was prepared using the catechin extract obtained under the optimum conditions as described above, and the catechin granular tea containing the catechin component was developed using the catechin component as well as the optimal extraction condition of the catechin in the blancia salmity. .

KR 10-0890945 B1 2009.03.27. KR 10-0955726 B1 2010.05.03. KR 10-2011-0085433 A 2011.07.27.

Thesis 1: Master's Thesis, Keimyung University Journal of the Korean Society of Food Hygiene and Safety, Vol22, No2, pp 120 ~ 126 (2007) Journal of the Korean Society of Food Science and Technology, Vol. 40, No. 4, 2008 p 449 ~ 454, 2008, Vol. 40, No. 4, p 455 ~ 459 Journal of the Korean Society for Laboratory Animal Science, 2008 (24), p 83 ~ 86 A review of latest reserch findings on the healthpromotion properties of tea, Christiane J, Dufresne, Edward R. Farnworth, J. Nutr. Bichem, Dec. 2001, 404-421 Journal of Korean Tea Culture Association, March-April 2000, 30-33 Journal of the Korean Society for Laboratory Animal Science, 2008 Symposium, PS-B-8 Journal of the Korean Society of Acquired Common Infectious Diseases, 2009, P-68

An object of the present invention is a catechin-containing blend, which has the effect of eliminating active oxygen in the body, controlling obesity, and improving body fat, including blancin salvia extract, dietary fiber, embryonic soybean extract and barley extract powder containing catechin. Producing method of the extract of sia salmity extract, blancia salmity extract containing catechin prepared thereby, high concentration catechin granule tea comprising the same and a composition for the removal of free radicals, control of obesity and body fat improvement will be.

In order to achieve the above object, the present invention provides the following means.

The present invention comprises the steps of adding and extracting ethanol to the blancia salmity (step 1); Filtering the extract (step 2); And drying the filtered extract for 4 to 5 hours (step 3); It provides a method for producing a blancia salmity extract containing catechin, characterized in that it comprises a.

Step 1 is characterized in that 30 to 50 mL of ethanol per 1 g of the blendia salmity is added and extracted for 1 to 2 hours at 88 ~ 90 ℃.

In another aspect, the present invention provides a blancia salmity extract containing catechin, characterized in that the production method.

In addition, the present invention, 2 to 3 parts by weight of dietary fiber, 0.5 to 1 part by weight of chaga extract powder, 0.1 to 0.4 parts by weight of embryonic soybean extract, based on 100 parts by weight of the Blencia salmity extract containing the catechin And it provides a high concentration catechin granules tea containing blancia salmity extract containing catechin, characterized in that containing 1 to 3 parts by weight of barley extract powder.

0.1 to 0.3 parts by weight of vitamin C, 0.1 to 0.3 parts by weight of citric acid, 1 to 5 parts by weight of enzymatically treated stevia, and mixed grain flavor based on 100 parts by weight of the high concentration of catechin granule tea containing the catechin-containing blancia salmity extract It is characterized by additionally adding ~ 6 parts by weight.

In addition, the present invention, any one or more selected from the group consisting of dietary fiber, chaga mushroom extract powder, embryonic soybean fermentation extract and barley extract powder with respect to 100 parts by weight of the Blencia salmity extract containing the catechin 0.1 ~ 20 It provides a food composition for removing free radicals in the body, controlling obesity and improving body fat, comprising a weight part.

High concentration catechin granular tea comprising the catechin-containing blancia salmity extract according to the present invention is effective in removing free radicals, controlling obesity, improving body fat and antiviral effect.

The high concentration catechin granular tea containing the catechin-containing blancia salmity extract contains more than four times the catechins compared to the conventional green tea, and has a much richer antioxidant effect than the general green tea or tea drink.

In addition, the high concentration of catechin granite tea containing the catechin blancia salmity extract is a powder type that is shaken in cold water and can be enjoyed easily and fresh anytime and anywhere. You can satisfy young women's taste with various attractive tastes such as freshness and refreshing taste. It will be called an excellent invention in the health food or diet industry by adding a diet improvement function to it.

1 is a graph showing a comparison of the catechin content of the blancia salmity extract and green tea extract according to the present invention.

Hereinafter, the present invention will be described in detail.

Catechin is the most famous of the green tea constituents, a polyphenol compound called tannin. Catechin is the most common constituent of tea. Ringed substance, belonging to condensed tannins and having the basic structure of Flavan-3-ol. Due to its chemical structure, it has a lot of hydroxyl groups (-OH), so it is easily combined with various materials. There are eight kinds of catechin components in green tea, of which (-)-EC, (-)-EGC, (-)-ECg and (-)-EGCg are referred to as general tea catechins.

The catechin compound is [2R, 3R] -2- [3,4,5-Trihydiroxyphenyl] -3,4-dihydr o-1 [2H] -benzopyran-3,5,7-triol 3- [3, 4,5-trihydroxybenzene]. To date, there have been many studies on catechins, and the focus has been on antioxidant, anticancer and cholesterol lowering effects.

Recently, the area of interest has been expanded to various areas such as obesity control, calculus removal, anti-allergy, dermatitis improvement, cold prevention, etc. In addition, research on atherine's arteriosclerosis and anti-tumor effect has been actively conducted.

First, the preparation method of the blancia salmity extract containing catechin according to the present invention.

Method for producing a blancia salmity extract containing catechin of the present invention,

Adding and extracting ethanol to the blancia salmity (step 1);

Filtering the extract (step 2); And

Drying the filtered extract (step 3);

.

Step 1 is a step of adding 30 ~ 50mL of ethanol per 1 g of the blendia salmity and extract for 1 to 2 hours at 88 ~ 90 ℃.

Step 2 is filtering the extract with a filter paper (Filter papers Whatman, 90mm Dia, 2.5㎛).

Step 3 is a step of drying the filtered extract for 4 to 5 hours.

The present invention provides a blancia salmity extract containing catechin prepared by the above method.

In another aspect, the present invention provides a high concentration catechin granular tea containing the Blencia Salmity extract containing the catechin.

High concentration catechin granule tea comprising the catechin-containing blancia salmity extract of the present invention is 2 to 3 parts by weight of dietary fiber, chaga mushroom extract powder 0.5 to 1 based on 100 parts by weight of blancia salvia extracts containing catechin It includes weight parts, embryonic soybean extract 0.1-0.4 parts by weight and barley extract powder 1-3 parts.

The dietary fiber is cha cha blood, cellulose, hemicellulose, crystalline cellulose, lignin, pectin, alginic acid, polymanneuroic acid, guar gum, gum arabic, arabinogalactan, konjac mannan, inulin, levan, polytextrose, indigestible maltotextile One or more selected from among them can be used.

The chaga extract powder is boiled with 8000cc of water in a water heater and cooled to 50 ~ 60 ℃, 800g bag containing chaga mushroom powder in 50 ~ 60 ℃ water and extracted for 48 hours after the chaga mushroom powder After removing the bag, the extract is dried for 3 to 5 hours through a hot air dryer, it can be used to extract powder powdered to 50mesh. The chaga extract powder contains a large amount of beta glucan component and is excellent in removing harmful oxygen.

The embryonic soybean fermentation extract is 24 ~ at a temperature of 25 ~ 45 ℃ by any one or more microorganisms selected from the group consisting of microorganisms of the genus Saccharomyces ( Saccharomyces ) and Isschenchenia ( Isatchenkia ) Fermentations incubated for 72 hours can be used.

The barley extract powder is cut to the appropriate size by washing barley, immersed in water for 24 hours, roasted soaked barley at 80 ℃ for 45 minutes, steamed for 2 to 3 hours at 140 ~ 160 ℃, hot air It can be used by drying through a dryer to remove moisture and crushing into 12 ~ 50mesh.

In addition, 0.1 to 0.3 parts by weight of vitamin C, 0.1 to 0.3 parts by weight of citric acid, 1 to 5 parts by weight of enzymatically treated stevia and mixed grains based on 100 parts by weight of the high concentration catechin granule tea containing the catechin-containing blancia salmity extract 2 to 6 parts by weight of fragrance may be added.

The vitamin C is a vitamin that is good for beauty and has a strong reducing effect has an antioxidant effect and has the effect of inhibiting the production of free radicals that cause skin aging.

The citric acid is included to add flavor and aroma of granular tea.

The enzyme-treated stevia (Glucosyl Stevia) is a micro-improved product to reduce the intrinsic bitter taste of stevioside by adding glucose to sugar extract by using a sugar transfer enzyme.

The fragrance may use any one commonly used in the art, Preferably mixed grain fragrance can be used.

High concentration catechin granule tea containing the catechin-containing blancia salmity extract has the effect of eliminating free radicals, controlling obesity and improving body fat. The high concentration catechin granule tea containing the catechin-containing blancia salmity extract is in the form of a powder stick-type small package which is added to water, and can be prepared as a general drinkable drinking water and a supplement for adding to water.

In another aspect, the present invention provides a food composition for the removal of free radicals in the body, control of obesity and body fat improvement, including the extract of blancia salmityum containing the catechin.

In the body containing the active oxygen removal, obesity control and body fat improvement composition containing the catechin-containing blancia salmianti extract, dietary fiber, chaga extract powder based on 100 parts by weight of blancia-salencian extract containing catechin It is made by additionally adding 0.1-20 parts by weight of any one or more selected from the group consisting of fermented soybean extract and barley extract powder.

Hereinafter, the constitution and effects of the present invention will be described in more detail through examples. These embodiments are only for illustrating the present invention, and the scope of the present invention is not limited by these embodiments.

Blensia Salmonity was supplied as a dry powder form from Lucky Pharmaceutical Co., Ltd., 40 mL of ethanol per 1 g of the Blensia Salmonity was added and extracted at 88 ° C. for 2 hours to obtain a Blensia Salmonity extract. Then, the filter paper (Filter papers Whatman, 90mm Dia, 2.5㎛, Whatman international Ltd.) was filtered, dried for 5 hours in a moisture dryer to dry the moisture of the filtered extract, containing catechin Blensia Salmon Tea extract was prepared.

Comparative Example 1

Brown rice green tea of D Company was purchased, and brown rice green tea extract was prepared using the same preparation method as in Example 1.

[Experimental Example 1]

The catechin component content was measured by separating the catechin from the Blencia salmity extract containing the catechin prepared in Example 1 and the brown rice green tea extract of Comparative Example 1, and the results are shown in Table 1 and FIG. .

division Catechin Content (mg / g) Example 1 84 Comparative Example 1 22

Looking at Table 1, it can be seen that the Blencia salmity extract containing the catechin prepared in Example 1 contains about 4 times more catechin content than brown rice extract of Comparative Example 1.

3 parts by weight of dietary fiber, 1 part by weight of chaga mushroom extract powder, 0.3 parts by weight of embryonic soybean extract and 2 parts by weight of barley extract powder A mixture was prepared.

As the dietary fiber, indigestible maltotextrin was used.

The chaga extract powder is boiled with 8000cc of water in a water heater, cooled to 55 ℃, 800g bag containing chaga mushroom powder in 55 ℃ water and extracted for 48 hours after removing the bag containing chaga powder And, the extract was dried for 4 hours through a hot air dryer and then used to powder into 50mesh.

The embryonic soybean fermentation extract is added to the medium containing 40g of embryos, 4g of soybeans and 500g of water and stirred by adding 0.05% (w / w) of protease and mixing the raw materials, 1% Saccharomyces cerevisiae in the medium A fermentation product was used which was incubated with aeration (120 rpm) for 48 hours at a temperature of 40 ° C. after inoculation (w / w).

The barley extract powder is cut to the appropriate size by washing barley, immersed in water for 24 hours, roasted soaked barley at 80 ℃ for 45 minutes, steamed at 150 ℃ for 2 hours, and dried by hot air dryer Water was removed, and used to crush into 12 ~ 50mesh.

0.2 parts by weight of vitamin C, 0.2 parts by weight of citric acid, 3 parts by weight of enzymatically treated stevia, and 4 parts by weight of mixed grains were added to the mixture, and then molded by a granulator to contain catechin. A high concentration catechin granule tea comprising the extract was prepared.

[Comparative Example 2]

Commercial brown rice green tea of D company was purchased.

[Experimental Example 2]

Sensory evaluation was performed on the taste and aroma of the high concentration catechin granular tea containing the blancin salmity extract containing catechin prepared in Example 2 and the brown rice green tea of Comparative Example 2, and are shown in Table 2. Granule tea of Example 2 was to drink 1 g 5g in 500ml bottled water, brown rice green tea of Comparative Example 2 was to drink tea bag in 70 ℃ water.

division Taste (20 Ratings) Incense (20 Ratings) Example 2 12 people 11 people Comparative Example 2 5 people 7 people Remarks 3 people (similar) 2 people (similar)

Referring to Table 2, it was found that the high concentration of catechin granulated tea containing the Blencia Salmity extract containing the catechin prepared in Example 2 was superior to brown rice green tea in taste and aroma.

[Experimental Example 3]

Clinical trials were conducted in 50 subjects using a high concentration of catechin granule tea containing the Blencia salmity extract containing the catechin prepared in Example 2. The age distribution of subjects was in their 20s, 10s, 10s, 10s, 40s, 10s and 50s, and 10s in their 60s. The occupations included 25 housewives, 10 office workers and 15 freelancers. The subjects were allowed to drink 5 g of the granulated tea in 500 ml bottled water once a day for 1 month, and the weight loss effect is shown in Table 3.

Weight loss Number of subjects Less than 1kg 14 1-3 kg 22 3-5 kg 9 5-7 kg 5 Side Effect none

Looking at Table 3, it can be seen that the high concentration of catechin granule tea containing the Blencia salmity extract containing the catechin prepared in Example 2 is excellent in weight loss effect but without side effects.

Claims (6)

Adding and extracting ethanol to the blancia salmity (step 1);
Filtering the extract (step 2); And
Drying the filtered extract for 4 to 5 hours (step 3);
Method for producing a blancia salmity extract containing catechin, characterized in that it comprises a.
The method of claim 1, wherein step 1,
30 to 50 mL of ethanol per 1 g of Blancia Salmonity is added and extracted for 1 to 2 hours at 88 to 90 ° C.
Blensia salmity extract containing catechin, characterized in that prepared by the method of any one of claims 1 or 2.
2 to 3 parts by weight of dietary fiber, 0.5 to 1 part by weight of chaga extract powder, 0.1 to 0.4 parts by weight of embryonic soybean extract, and barley extract powder, based on 100 parts by weight of blancia salmity extract containing catechin of claim 3 High concentration catechin granular tea comprising blancia salmity extract containing catechin, characterized in that it comprises 1-3 parts by weight.
5. The method of claim 4,
0.1 to 0.3 parts by weight of vitamin C, 0.1 to 0.3 parts by weight of citric acid, 1 to 5 parts by weight of enzymatically treated stevia, and mixed grain flavor 2 High concentration catechin granular tea comprising blancia salmity extract containing catechin, characterized in that additionally add ˜6 parts by weight.
Claims 1 to 100 parts by weight of the blancin Salencian extract containing catechins containing any one or more selected from the group consisting of dietary fiber, chaga mushroom extract powder, embryonic soybean extract and barley extract powder 0.1 to 20 parts by weight Food composition for the removal of free radicals in the body, control of obesity and body fat improvement, comprising a catechin-containing blancia salmity extract.

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