KR20130135111A - Multi-channel device for distributing liquid sample, device for extracting nucleic acid comprising the same, and method for extracting nucleic acid using the same - Google Patents
Multi-channel device for distributing liquid sample, device for extracting nucleic acid comprising the same, and method for extracting nucleic acid using the same Download PDFInfo
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Abstract
An embodiment of the present invention relates to a multi-channel liquid dispensing apparatus, a nucleic acid extracting apparatus including the same, and a nucleic acid extracting method using the same. In this respect, in performing various biological reactions using a thin- It is possible to quickly dispense and dispense the same very small amount of liquid to the small one or more inflow portions and to accurately distribute a very small amount of liquid to the one or more inflow portions by only one user operation, Can be significantly shortened to allow rapid progression of a variety of subsequent biological detection or analysis reactions.
Description
TECHNICAL FIELD The present invention relates to a liquid dispensing apparatus for accurately and simultaneously distributing a biological sample or a reagent and a liquid to a thin-film microfluidic chip having a liquid inlet, a nucleic acid extracting apparatus including the same, and a nucleic acid extracting method using the same.
Recently, techniques for extracting nucleic acids from biological samples such as cells, bacteria, or viruses have been widely used in connection with nucleic acid amplification reaction techniques in order to diagnose, treat, or prevent disease at a gene level. In addition to the diagnosis, treatment, or prevention of diseases, technologies for extracting nucleic acids from biological samples in various fields such as customized drug development, forensic medicine, and environmental hormone detection are required. As an example of a conventional nucleic acid extraction technique, there is a method of treating a sample containing cells with SDS or proteinase K, followed by solubilization, and denaturing the protein with phenol to purify the nucleic acid. However, since the phenol extraction method requires a lot of processing steps, it takes a lot of time and the efficiency of the nucleic acid extraction depends greatly on the experience and the skill of the researcher. In recent years, a kit using silica or glass fiber that specifically binds to nucleic acid has been used to solve this problem. Since the silica or glass fiber has a low binding ratio with proteins and cellular metabolites, a relatively high concentration of nucleic acid can be obtained. Such a method is advantageous compared with the phenol method, but since it uses a chaotropic reagent or ethanol which strongly inhibits the enzyme reaction such as PCR, it is necessary to completely remove these substances, This is a very cumbersome and time-consuming disadvantage. A method of directly purifying nucleic acids using recent filters is disclosed in WO 00/21973, which involves passing a sample through a filter to adsorb the cells to the filter, dissolving the adsorbed cells in the filter, After filtration, the nucleic acid adsorbed on the filter is washed and eluted. However, in order to elute the nucleic acid after adsorbing the cells on the filter, there is a problem in that a filter must be selected according to the type of the cell, and there are disadvantages that the apparatuses are large and complex and can not be used easily by researchers.
In addition, it is necessary to provide a device for injecting a liquid such as a very small sample or reagent into a reaction vessel during various biological reactions. Conventional reaction vessels are mostly tube-shaped, and furthermore, multi-tubes in which a plurality of tubes having a very small volume are arranged and arranged are also used. Thus, devices commonly used to inject, mix, or withdraw liquids such as very small amounts of sample or reagent into such reaction vessels are pipettes and tips. However, since the pipette and the tip are not satisfactory in accuracy by adjusting the amount of liquid to be injected into the reaction vessel by a manual operation, particularly when injecting a liquid into one or more small-sized inflow portions of the thin- It is very troublesome that a tip having a fine outlet and a pipette designed accordingly are required. In addition, when the reaction is performed using a thin-film microfluidic chip having a plurality of reaction channels, the pipette can not accurately and quickly dispense the same sample or reagent, which is very troublesome. Therefore, in a biological reaction using a thin-film shaped microfluidic chip, it is possible to accurately and accurately distribute a very small amount of sample or reagent to one or more small-sized influent portions quickly and accurately, to improve user convenience and to perform a rapid reaction A liquid dispensing apparatus capable of discharging liquids is required.
One embodiment of the present invention is a method for rapidly and accurately dispensing a very small amount of liquid such as a biological sample or reagent to at least one inlet of a thin film microfluidic chip having at least one reaction channel, , A multi-channel liquid distributor, a nucleic acid extracting apparatus including the same, and a nucleic acid extracting method using the same.
A first embodiment of the present invention is a thin film transistor comprising: a thin film substrate; A single liquid inlet disposed at one end region of the substrate; And one or more even liquid outlets disposed in the other end region of the substrate and in fluid communication with the single liquid inlet through a channel, the channel having one end connected to the single liquid inlet side And the other end is divided into two halves so as to distribute the flow rate by half, and has a channel pattern connected to the liquid outlet side, wherein the multi-channel liquid distributor comprises at least one unit channel region .
In the first embodiment of the present invention,
The liquid discharge port is implemented
A second embodiment of the present invention is a microfluidic chip (1) in the form of a thin film having at least one to at least two reaction channels each having an inlet and an outlet at both ends thereof, Channel liquid dispensing apparatus according to the first embodiment of the present invention for down-infusing liquid into at least one reaction channel, and having a liquid outlet corresponding to the number of the at least one inlet; And fluid delivery means for fluidly connecting one or more inflow portions of the microfluidic chip to at least one liquid outlet of the multi-channel liquid distribution device.
A third embodiment of the present invention is directed to extracting nucleic acid from a biological sample comprising an inlet, a channel region connected to the inlet, and an outlet connected to the channel region, wherein the channel region is introduced through the inlet A microfluidic chip for nucleic acid extraction in the form of a thin film, the microfluidic chip having at least one reaction channel, the microfluidic chip for nucleic acid extraction comprising: a heating unit configured to transfer externally obtained heat to the biological sample; A multi-channel liquid distributor according to the first embodiment of the present invention, having a liquid outlet coinciding with the number of the at least one inlet; And fluid delivery means for fluidly connecting at least one inlet of the microfluidic chip and at least one liquid outlet of the multi-channel liquid distributor.
In a third embodiment of the present invention,
A chip outlet region end mount adapted to fixably mount at least one outlet region end of the microfluidic chip, at least one upward liquid inlet corresponding to an upper end of at least one outlet of the microfluidic chip, And a liquid storage vessel having one or more liquid storage chambers fluidly connected to the upward liquid intake.
The microfluidic chip may include a first filter disposed in a first channel region connected to the inlet unit and disposed in a second channel region connected to the heating unit and capable of passing a substance having a size corresponding to nucleic acid .
The microfluidic chip may include a first filter disposed in a first channel region connected to the inlet portion and disposed in a second channel region connected to the heating unit and capable of passing a substance having a size corresponding to nucleic acid, And a nucleic acid separator disposed in a third channel region connected to the first filter and having a nucleic acid binding substance capable of specifically binding to the nucleic acid.
The microfluidic chip may include a first filter disposed in a first channel region connected to the inlet portion and disposed in a second channel region connected to the heating unit and capable of passing a substance having a size corresponding to nucleic acid, And a nucleic acid separator disposed in a third channel region connected to the first filter and having a nucleic acid binding substance capable of specifically binding with the nucleic acid, the nucleic acid separator being disposed in a fourth channel region connected to the nucleic acid separator, And a second filter capable of passing a substance having a size corresponding to the nucleic acid.
The microfluidic chip may further include a nucleic acid separator disposed in a channel region connected to the heating unit and having a heating portion disposed in a channel region connected to the inflow portion and having a nucleic acid binding material capable of specifically binding to the nucleic acid, .
The microfluidic chip may further include a nucleic acid separator disposed in a channel region connected to the heating unit and having a heating unit disposed in a channel region connected to the inlet unit and having a nucleic acid binding material capable of specifically binding with the nucleic acid, And a second filter disposed in a channel region connected to the nucleic acid separation unit and capable of passing a substance having a size corresponding to the nucleic acid.
The fourth embodiment of the present invention includes the steps of providing a nucleic acid extracting apparatus according to the third embodiment of the present invention; Injecting a biological sample or reagent into said microfluidic chip for nucleic acid extraction through said multi-channel liquid distributor and fluid delivery means; And driving the microfluidic chip for nucleic acid extraction to extract nucleic acid from the biological sample.
The fifth embodiment of the present invention provides a nucleic acid extracting apparatus according to the third embodiment of the present invention; Injecting a biological sample or reagent into said microfluidic chip for nucleic acid extraction through said multi-channel liquid distributor and fluid delivery means; Driving the microfluidic chip for nucleic acid extraction to extract nucleic acid from the biological sample; And storing the nucleic acid extracted product in a liquid storage chamber of the liquid storage container.
An embodiment of the present invention relates to a multi-channel liquid dispensing apparatus, a nucleic acid extracting apparatus including the same, and a nucleic acid extracting method using the same. In this respect, in performing various biological reactions using a thin- It is possible to quickly dispense and dispense the same very small amount of liquid to the small one or more inflow portions and to accurately distribute a very small amount of liquid to the one or more inflow portions by only one user operation, Can be significantly shortened to allow rapid progression of a variety of subsequent biological detection or analysis reactions.
1 illustrates a multi-channel liquid dispensing apparatus in accordance with an embodiment of the present invention.
Figures 2 to 3 show the unit channel area of the channels of the multi-channel liquid distributor according to Figure 1.
Figures 4 to 5 schematically illustrate a microfluidic chip according to an embodiment of the present invention.
Figure 6 illustrates a multi-channel liquid dispensing device in accordance with an embodiment of the present invention.
FIGS. 7 to 10 illustrate a microfluidic chip according to an embodiment of the present invention and illustrate a nucleic acid extraction method using the same.
11 to 13 show a liquid storage container according to an embodiment of the present invention.
FIG. 14 shows a flow path of a liquid such as a biological sample or a reagent in a state where the microfluid chip and the liquid storage container are combined according to an embodiment of the present invention.
Figure 15 illustrates the path of movement of a liquid, such as a biological sample or reagent, in conjunction with a multi-channel liquid distributor, microfluidic chip, and liquid reservoir according to one embodiment of the present invention.
16 to 17 show results of nucleic acid extraction experiments using a third-party nucleic acid extracting apparatus and a nucleic acid extracting apparatus according to an embodiment of the present invention, respectively.
Hereinafter, an embodiment of the present invention will be described in detail with reference to the accompanying drawings. The following description is only for the purpose of easy understanding of the embodiments of the present invention, and is not intended to limit the scope of protection of the present invention from such description.
1 shows a multi-channel liquid dispensing
1, the multi-channel
The
The multi-channel liquid distributor (2) is for simultaneously distributing the same amount of sample or reagent in a single operation to a reaction vessel having at least one inlet. Thus, the multi-channel
Figures 2-3 illustrate the
2, the
Furthermore, the
Figures 4 to 5 schematically illustrate a
The
Figure 6 illustrates a multi-channel liquid dispensing device in accordance with an embodiment of the present invention.
6, a multi-channel liquid distribution injection apparatus according to an embodiment of the present invention includes a microfluidic chip having a thin film-shaped microfluidic chip having at least one and at most two reaction channels each having an inlet and an outlet, (1, 2, 3, 4) for injecting liquid downward into the at least one reaction channel through the at least one inlet (10), wherein the liquid outlets (4000, Channel liquid dispensing device (2) comprising: a) a multi-channel liquid dispensing device (2); And fluid delivery means (4) for fluidly connecting at least one inlet (10) of the microfluidic chip (1) and at least one liquid outlet (4000) of the multi-channel liquid distributor (2) do. In this case, the
FIGS. 7 to 10 illustrate a microfluidic chip according to an embodiment of the present invention and illustrate a nucleic acid extraction method using the same. 7 to 10, the microfluidic chip according to an embodiment of the present invention can be used for nucleic acid extraction. Hereinafter, in Figs. 7 to 10, the microfluidic chip is referred to as "microfluidic chip for nucleic acid extraction ".
The microfluidic chip for nucleic acid extraction is a structure for extracting nucleic acid, that is, an inlet, an outlet, a channel connecting the inlet and the outlet, a first filter, 2 filter or the like is implemented in units of millimeters (mm) or micrometers (탆).
7A, a microfluidic chip for nucleic acid extraction according to an embodiment of the present invention includes an
The microfluid chip for nucleic acid extraction according to an embodiment of the present invention shown in FIG. 7B has a
The biological sample may be a biological sample containing a nucleic acid such as DNA or RNA and may be, for example, a liquid sample including animal cells, plant cells, pathogens, fungi, bacteria, viruses and the like.
The inlet (10) is a portion where the biological sample or a solution for nucleic acid extraction is introduced into the microfluidic chip, and the outlet (60) is a portion for extracting the nucleic acid, the solution for nucleic acid extraction, And other waste is discharged to the outside of the microfluidic chip. In this case, the
The
The
The nucleic
The
8 is a cross-sectional view of a microfluidic chip for nucleic acid extraction according to an embodiment of the present invention.
8 is a cross-sectional view of a microfluidic chip for nucleic acid extraction according to an embodiment of the present invention. The microfluidic chip for nucleic acid extraction according to an embodiment of the present invention comprises a
9 is a schematic view of a nucleic acid extracting apparatus equipped with a microfluidic chip for nucleic acid extraction according to an embodiment of the present invention.
Referring to FIG. 9, a nucleic acid extracting apparatus according to an embodiment of the present invention includes: a
The nucleic acid extracting apparatus is implemented to perform all steps for nucleic acid extraction in a state where the
The
The
The
The
Meanwhile, the nucleic acid extracting apparatus according to an embodiment of the present invention includes an electronic control module (not shown) for automatically controlling the
10 is a flowchart of a nucleic acid extraction method according to an embodiment of the present invention. Specifically, FIGS. 10A to 10D show various nucleic acid extraction methods based on the
Referring to FIG. 10A, a method of extracting a nucleic acid from a biological sample according to an embodiment of the present invention includes: providing a microfluidic chip for nucleic acid extraction according to FIG. 7F (providing a microfluidic chip); Introducing a biological sample selected from the group consisting of cells, bacteria, and virus (biological sample introduction step) through the inlet of the microfluidic chip; Moving the introduced biological sample to a heating section of the microfluidic chip and then applying heat to the heating section of the microfluidic chip to lyse the biological sample (biological sample dissolution step); Separating the nucleic acid from the dissolving material through a nucleic acid binding material (membrane) (nucleic acid separation step); As a selectable step, there is a step of removing foreign substances generated in the nucleic acid separation process (a foreign substance removing step); And a step (nucleic acid extraction step) of transferring the nucleic acid to the outflow portion and then extracting the nucleic acid through the outflow portion.
Referring to FIG. 10B, a method of extracting nucleic acid from a biological sample according to an embodiment of the present invention includes the steps of providing a microfluidic chip for nucleic acid extraction according to FIG. 7B or 7C (providing a microfluidic chip); Introducing a biological sample selected from the group consisting of cells, bacteria, and virus (biological sample introduction step) through the inlet of the microfluidic chip; Moving the introduced biological sample to a heating section of the microfluidic chip and then applying heat to the heating section of the microfluidic chip to lyse the biological sample (biological sample dissolution step); Moving the material obtained from the dissolving step to a first filter of the microfluidic chip, passing through the first filter, and removing material that has not passed through the first filter (filtration through a first filter ); Separating the nucleic acid from the substance that has passed through the first filter (nucleic acid separation step); As a selectable step, there is a step of removing foreign substances generated in the nucleic acid separation process (a foreign substance removing step); And a step (nucleic acid extraction step) of transferring the nucleic acid to the outflow portion and then extracting the nucleic acid through the outflow portion.
Referring to FIG. 10C, a method of extracting nucleic acid from a biological sample according to an embodiment of the present invention includes the steps of providing a microfluidic chip for nucleic acid extraction according to FIG. 7G (providing a microfluidic chip); Introducing a biological sample selected from the group consisting of cells, bacteria, and virus (biological sample introduction step) through the inlet of the microfluidic chip; Moving the introduced biological sample to a heating section of the microfluidic chip and then applying heat to the heating section of the microfluidic chip to lyse the biological sample (biological sample dissolution step); Separating the nucleic acid from the dissolution material through a nucleic acid binding material (bead) (nucleic acid separation step); As a selectable step, there is a step of removing foreign substances generated in the nucleic acid separation process (a foreign substance removing step); Separating the nucleic acid from the nucleic acid binding material, transferring the separated nucleic acid to the second filter, and passing the separated nucleic acid through a second filter (filtering step through a second filter); And a step (nucleic acid extraction step) of transferring the nucleic acid to the outflow portion and then extracting the nucleic acid through the outflow portion.
Referring to FIG. 10d, a method of extracting nucleic acid from a biological sample according to an embodiment of the present invention includes the steps of providing a microfluidic chip for nucleic acid extraction according to FIG. 7d or 7e (providing a microfluidic chip); Introducing a biological sample selected from the group consisting of cells, bacteria, and virus (biological sample introduction step) through the inlet of the microfluidic chip; Moving the introduced biological sample to a heating section of the microfluidic chip and then applying heat to the heating section of the microfluidic chip to lyse the biological sample (biological sample dissolution step); Moving the material obtained from the dissolving step to a first filter of the microfluidic chip, passing through the first filter, and removing material that has not passed through the first filter (filtration through a first filter ); Separating the nucleic acid from the dissolution material through a nucleic acid binding material (bead or membrane) (nucleic acid separation step); As a selectable step, there is a step of removing foreign substances generated in the nucleic acid separation process (a foreign substance removing step); Separating the nucleic acid from the nucleic acid binding material, transferring the separated nucleic acid to the second filter, and passing the separated nucleic acid through a second filter (filtering step through a second filter); And a step (nucleic acid extraction step) of transferring the nucleic acid to the outflow portion and then extracting the nucleic acid through the outflow portion.
11 to 13 show a liquid storage container according to an embodiment of the present invention.
11, the
Based on the above-described nucleic acid extracting apparatus, an embodiment of the present invention can provide a convenient, quick and efficient ultra-fast nucleic acid extracting method. For example, the first nucleic acid extraction method comprises the steps of: providing the nucleic acid extraction apparatus described above; Injecting a biological sample or reagent into said microfluidic chip for nucleic acid extraction through said multi-channel liquid distributor and fluid delivery means; And driving the microfluidic chip for nucleic acid extraction to extract nucleic acid from the biological sample. The second nucleic acid extraction method may further include the steps of: providing the nucleic acid extraction device; Injecting a biological sample or reagent into said microfluidic chip for nucleic acid extraction through said multi-channel liquid distributor and fluid delivery means; Driving the microfluidic chip for nucleic acid extraction to extract nucleic acid from the biological sample; And storing the nucleic acid extracted product in a liquid storage chamber of the liquid storage container.
Hereinafter, in Examples 1 and 2, the amount and time of the nucleic acid extract were determined while extracting the nucleic acid from the biological sample in comparison with the third-party nucleic acid extracting apparatus (Qiagen), and further, The reliability of the result of the extract was confirmed again.
Example 1. Identification of nucleic acid extraction yield and run time
First, DNA is extracted from a tubercle main cell using a general tube included in a third-party product and a
The steps of nucleic acid extraction using a third-party nucleic acid separator are as follows. The Mycobacterium tuberculosis main cell was prepared and mixed with 6% NaOH and 4% NaLC at a ratio of 1: 1: 1 to prepare a sample solution. The sample solution was then centrifuged to remove supernatant (10 min, 7500 rpm, 4 캜). Then, 20 占 퐇 of Proteinase K was added to the sample solution and left at 56 占 폚 until the sample solution became transparent. Then, 200 μl of AL buffer was added to the sample solution, mixed for 15 seconds, and left at 56 ° C for 10 minutes. The sample solution was then transferred to a column and centrifuged (8000 rpm) for 1 minute. Then, 500 μl of
Subsequently, nucleic acids were extracted from the same M. tuberculosis cell using the
The Mycobacterium tuberculosis main cell was prepared and mixed with 6% NaOH and 4% NaLC at a ratio of 1: 1: 1 to prepare a sample solution. Thereafter, it was introduced into at least one inlet of a microfluidic chip for nucleic acid extraction (25 x 72 x 2 mm, silica bead (OPS Diagnostics, LLC), filter (Whatman)) according to Fig. 300 [micro] l of silica gel and 1X DNA binding buffer were introduced into the microfluidic chip inflow section according to the instant embodiment of the present invention, and then the heating section of the microfluid chip according to the instant embodiment of the present invention was heated to 95 [ . Thereafter, wastes in the sample solution were removed through an inlet of the microfluidic chip according to a temporary example of the present invention, and 100 μl of an elution buffer was introduced. In this case, the reagent was introduced into the microfluidic chip for nucleic acid extraction using the
As a result of the above experiment, when the
Example 2. Third party products and work of the invention In the embodiment Obtained by the nucleic acid extraction method according to DNA Polymerase chain reaction of products PCR ) result
In order to ensure the reliability of the DNA product obtained in Example 1, PCR was carried out based on the DNA product.
The polymerase chain reaction (PCR) was performed using a third-party product (Bio-Rad: CFX connect equipment). PCR samples and reagents for PCR were prepared by mixing 10 μl of real-time PCR mixed solution (TOYOBO SYBR qPCR mix), 2 μl of forward primer (μl), reverse primer (Reverse) A total of 20 microliters (占 퐇) including 2 microliters (쨉 l) of primer, 10 占), 1 microliter (쨉 l) of template DNA (1ng) and 5 占 퐇 l of distilled water . Thereafter, pre-denaturation step (95 ° C, 30 sec) was performed (1 cycle), denaturation step at 95 ° C for 5 sec and anealing & extension step at 72-65 ° C for 30 sec cycle.
FIG. 16 is a graph showing the results of real-time PCR on the nucleic acid-extracted products obtained using the nucleic acid extraction method and fluorescence of each PCR cycle. FIG. Figure 17 is a photograph of gel electrophoresis of the final PCR product. The graph curve of FIG. 16 is a PCR result curve (X axis: cycle, Y axis: fluorescence) of the DNA product by each nucleic acid extraction method.
(1) is a negative control group, and (2) is a positive control group.
The nucleic acid extracting method using the nucleic acid extracting apparatus according to an embodiment of the present invention can maintain or improve the result reliability of the nucleic acid extract product and significantly reduce the nucleic acid extracting step, I can confirm that I can do it.
Claims (12)
A single liquid inlet disposed at one end region of the substrate; And
At least one liquid outlet disposed in the other end region of the substrate and fluidly connected through the channel with the single liquid inlet;
, ≪ / RTI &
The channel includes at least one unit channel region having one end connected to the single liquid injection port side and the other end divided into two to distribute the flow rate to one half and having a channel pattern connected to the liquid discharge port side However,
Multi-channel liquid distributor.
The liquid discharge port is implemented pieces 2 N, wherein the channel and wherein the N or less the i-th unit of the channel region is 2 i-1 of start of the N units of the channel region formed by the 2 N of the liquid outlet from the single liquid inlet channel and the 2 i-1 from the start of the channel is divided into two branches each comprising a 2 i of branch channels for distributing the flow rate of 1/2, the first unit of the start of the channel section a channel-side terminal is the single liquid inlet is connected to the N-th unit as a channel region of the branch channel ends are respectively connected to the 2 N of the liquid outlet, wherein the N and i is the one which, the natural-channel liquid dispensing device.
The multi-channel liquid distributor according to any of the preceding claims, comprising a liquid outlet coinciding with the at least one inlet number; And
Fluid delivery means for fluidly communicating at least one inlet of the microfluidic chip and at least one liquid outlet of the multi-channel liquid distributor;
Channel liquid dispensing device.
The multi-channel liquid distributor according to any of the preceding claims, comprising a liquid outlet coinciding with the at least one inlet number; And
Fluid delivery means for fluidly communicating at least one inlet of the microfluidic chip and at least one liquid outlet of the multi-channel liquid distributor;
And a nucleic acid extracting device.
A chip outlet region end mount adapted to fixably mount at least one outlet region end of the microfluidic chip, at least one upward liquid inlet corresponding to an upper end of at least one outlet of the microfluidic chip, Further comprising a liquid reservoir having at least one liquid storage chamber fluidly connected to the upstream liquid inlet.
The microfluidic chip may include a first filter disposed in a first channel region connected to the inlet portion and disposed in a second channel region connected to the heating unit and capable of passing a substance having a size corresponding to the nucleic acid, Wherein the nucleic acid extracting device is a nucleic acid extracting device.
The microfluidic chip may include a first filter disposed in a first channel region connected to the inlet portion and disposed in a second channel region connected to the heating unit and capable of passing a substance having a size corresponding to nucleic acid, And a nucleic acid separator disposed in a third channel region connected to the first filter and having a nucleic acid binding substance capable of specifically binding to the nucleic acid.
The microfluidic chip may include a first filter disposed in a first channel region connected to the inlet portion and disposed in a second channel region connected to the heating unit and capable of passing a substance having a size corresponding to nucleic acid, And a nucleic acid separator disposed in a third channel region connected to the first filter and having a nucleic acid binding substance capable of specifically binding with the nucleic acid, the nucleic acid separator being disposed in a fourth channel region connected to the nucleic acid separator, And a second filter capable of passing a substance of a size corresponding to the nucleic acid.
Wherein the microfluid chip includes a nucleic acid separator disposed in a channel region connected to the heating unit and having a heating portion disposed in a channel region connected to the inflow portion and having a nucleic acid binding material capable of specifically binding with the nucleic acid, Wherein the nucleic acid extracting device is a nucleic acid extracting device.
The microfluidic chip may further include a nucleic acid separator disposed in a channel region connected to the heating unit and having a heating unit disposed in a channel region connected to the inlet unit and having a nucleic acid binding material capable of specifically binding with the nucleic acid, And a second filter disposed in a channel region connected to the nucleic acid separation unit and capable of passing a substance having a size corresponding to the nucleic acid.
Injecting a biological sample or reagent into said microfluidic chip for nucleic acid extraction through said multi-channel liquid distributor and fluid delivery means; And
Driving the microfluidic chip for nucleic acid extraction to extract nucleic acid from the biological sample;
/ RTI >
Injecting a biological sample or reagent into said microfluidic chip for nucleic acid extraction through said multi-channel liquid distributor and fluid delivery means;
Driving the microfluidic chip for nucleic acid extraction to extract nucleic acid from the biological sample; And
Storing the nucleic acid extracted product in a liquid storage chamber of the liquid storage container;
/ RTI >
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KR1020130060993A KR20130135111A (en) | 2012-05-30 | 2013-05-29 | Multi-channel device for distributing liquid sample, device for extracting nucleic acid comprising the same, and method for extracting nucleic acid using the same |
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- 2013-05-29 KR KR1020130060993A patent/KR20130135111A/en not_active Application Discontinuation
- 2013-05-30 WO PCT/KR2013/004775 patent/WO2013180494A1/en active Application Filing
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