KR20130092014A - A method for preparing the salted alaska pollack roe using by kelp oil - Google Patents

A method for preparing the salted alaska pollack roe using by kelp oil Download PDF

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KR20130092014A
KR20130092014A KR1020120013440A KR20120013440A KR20130092014A KR 20130092014 A KR20130092014 A KR 20130092014A KR 1020120013440 A KR1020120013440 A KR 1020120013440A KR 20120013440 A KR20120013440 A KR 20120013440A KR 20130092014 A KR20130092014 A KR 20130092014A
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weight
kelp
essential oil
water
cod roe
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KR1020120013440A
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KR101373384B1 (en
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조순영
라경민
최성미
최지연
임미진
조현덕
김도윤
최윤정
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강릉원주대학교산학협력단
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/30Fish eggs, e.g. caviar; Fish-egg substitutes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/02Preserving by means of inorganic salts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings, cooking oils
    • A23D9/02Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/65Addition of, or treatment with, microorganisms or enzymes
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00Production of fats or fatty oils from raw materials
    • C11B1/10Production of fats or fatty oils from raw materials by extracting

Abstract

PURPOSE: A manufacturing method of salted Pollack roe is provided to reduce the production cost and to prevent waste of food resources due to degeneration. CONSTITUTION: Salting water is manufactured by mixing 1-20 weight% of salt, 1-10 weight% of sugar, 1-10 weight% of water, and 0.01-5 weight% of refined rice wine based on 100 weight% of Pollack roe. Moisture is removed from Pollack roe, and the Pollack roe is dipped in the salting water. The impurities and moisture are removed from the dipped Pollack roe. Arid spice is manufactured by mixing 1-10 weight% of red pepper powder, 0.01-5 weight% of sugar, and 0.01-10% of kelp essential oil based on 100 weight% of Pollack roe. The Pollack roe with the impurities removed is seasoned. The arid spice is put on the seasoned Pollack roe. Water spice is manufactured by mixing 1-7 weight% of red pepper powder, 0.01-5 weight% of condiment, 1-10 weight% of garlic, 1-10 weight% of starch syrup, and 1-15 weight% of water based on 100 weight% of Pollack roe. The water spice is put on the Pollack roe seasoned with the arid spice.

Description

다시마 정유를 이용한 명란젓의 제조방법 {A method for preparing the salted Alaska pollack roe using by kelp oil}A method for preparing the salted Alaska pollack roe using by kelp oil}

본 발명은 천연 항균성 물질로서 다시마 정유를 이용하여 품질향상 및 저장성이 우수한 명란젓을 제조하는 방법에 관한 것이다.The present invention relates to a method for producing cod roe excellent in quality and shelf life using kelp essential oil as a natural antimicrobial substance.

[문헌 1] 김상무, 식품첨가제에 의한 저염 명란젓의 보존 효과, 한국식품영양과학회지, 25 (6), pp.937-943, 1996 [1] Kim, Sang Moo, Conservation effect of low salt cod roe by food additives, Korean Journal of Food Science and Nutrition, 25 (6) , pp.937-943, 1996

[문헌 2] 우원식. 2005. 천연물화학연구법. 서울대학교출판부. 167-235.[Document 2] Woo Wonsik. 2005. Natural Products Chemistry Research Act. Seoul National University Press. 167-235.

[문헌 3] 최한길외, 해양식물과 한의학, (주)학술정보, pp.18, 175-176, 2004[Reference 3] Choi, Han-Gil et al., Marine Plants and Oriental Medicine, Academic Information, pp.18, 175-176, 2004

젓갈은 어패류에 식염을 가하여 염장함으로써 부패균의 번식을 억제하고 자가소화효소 또는 미생물의 효소작용에 의해 육질을 분해시킨 우리나라 전통의 수산발효식품으로서 제조 공정이 단순하고 특별한 제조장치도 필요하지 않으며 저장후의 제품은 독특한 감칠 맛을 가지고 있어 예부터 오늘에 이르기까지 밑반찬이나 다시마치의 조미 소재로 많이 이용되고 있다(식품첨가제에 의한 저염 명란젓의 보존 효과, 한국식품영양과학회지, 25 (6), pp.937-943, 1996). Salted seafood is a traditional Korean fermented fish food that suppresses the growth of decayed bacteria by salting fish and shellfish and decomposes meat by the enzyme action of autodigestive enzyme or microorganism.The manufacturing process is simple and no special manufacturing equipment is needed. The product has a unique rich taste and is widely used as a seasoning material for side dishes and kelp from ancient times to today (preservation effect of low salted cod roe with food additives, Korean Journal of Food Science and Nutrition, 25 (6) , pp.937-943, 1996).

최근들어 건강 지향적인 식품의 소비가 증가함에 따라 젓갈제품은 염의 함량이 훨씬 낮은 양념 젓갈의 형태로 많이 이용되고 있는 실정이나, 이러한 저식염의 양념젓갈은 제조시에 발생되는 가장 큰 문제점 중의 하나가 젓갈의 저장성의 단축이다. 이에 저염 젓갈의 저장성을 연장하려는 연구가 폭넓게 이루어지고 있으나 아직까지 뚜렷한 해결방안이 마련되어 있지 않다. In recent years, as consumption of health-oriented foods has increased, the fermented product has been widely used in the form of seasoned salted fish which has a much lower salt content. However, such seasoned salted salted fish is one of the biggest problems It is shortening of storage stability of salted fish. To this end, researches to extend the shelf life of low salted salted fish have been widely conducted, but there are no clear solutions yet.

식물 정유는 특정식물체를 증류 또는 압착하여 얻어지는 휘발성의 향기성분으로서, 식물, 동물, 미생물들에서 방어기작, 신호전달, 2차대사를 위하여 생합성된 화학물질의 혼합물이다(우원식. 2005. 천연물화학연구법. 서울대학교출판부. 167-235)Plant essential oils are volatile fragrances obtained by distilling or compressing certain plants and are mixtures of chemicals synthesized for defense mechanisms, signaling and secondary metabolism in plants, animals and microorganisms (Woo Won Sik. 2005. Natural Products Chemistry Research Method) Seoul National University Press, 167-235)

특히, 다시마류(kelp; Laminaria)는 다시마과(Laminariaceae)에 속하는 갈조류의 다시마류(Laminaria spp.) 해초로서 유럽 북부해안에 자생하며, 아미노산인 라미나린(laminarine)과 다당류인 알긴산(alginic acid)가 다량 함유라며, 한국에서는 Agarum, Costaria, Ecklonia, Eisenia, Kjellmaniella, Laminaria 속 9종이 존재하며, 라미나리아속에는 다시마(Laminaria jponica) 및 애기 다시마(Laminaria religiosa) 및 개다시마 (Kjellmaniella crassifolia)가 동, 남해한 및 태평양 연안에 자생하며 일반성분으로는 지질, 단백질, 섬유질, 당질, 화분 등을 함유한 것으로 알려져 있다 (최한길외, 해양식물과 한의학, (주)학술정보, pp.64-65, 160-161, 2004).In particular, Kelp (Laminaria) is a seaweed of Laminaria spp. Belonging to the family Laminariaceae, which grows in the northern coast of Europe, and has the amino acid laminarine and polysaccharide alginic acid. Agarum, Costaria, Ecklonia, Eisenia, Kjellmaniella and Laminaria are 9 species in Korea, and Laminaria jponica and Laminaria religiosa and Kjellmaniella crassifolia are found in Laminaria. And native to the Pacific coast and are known to contain lipids, proteins, fiber, sugars, pollen, etc. (Han-gil Choi, Marine Plant and Oriental Medicine, Academic Information, pp.64-65, 160-161 , 2004).

이에 따라 본 발명자들은 젓갈의 맛과 질을 향상시킬 수 있는 방법에 관하여 연구하던 중 젓갈 변질 원인균의 증식을 억제시킬 것이라 기대되는 천연 항균성 물질로서 다시마 정유를 이용하여 제조한 명란젓이 시중에서 판매되는 명란젓보다 미생물, pH, 아미노태질소, VBN 및 TBA 측정결과, 저장성이 탁월함에 따라 변질로 인한 반품 및 폐기되는 양을 줄여 식량자원의 낭비를 막고, 제품의 생산 경비를 절감시킬 뿐만 아니라, 제품의 품질향상의 이점이 있어 다시마 정유를 이용한 명란젓의 제조방법을 본 발명에서 완성하였다.
Accordingly, the inventors of the present invention is a natural antibacterial substance that is expected to inhibit the proliferation of the causative agent of spoiled spoiled salt while researching how to improve the taste and quality of salted salted cod roe produced in kelp essential oil More microbial, pH, amino nitrogen, VBN, and TBA measurements result in greater shelf life, reducing the amount of returns and wastes resulting from alteration, not only saving food resources, but also reducing product production costs There is an advantage of improving the production method of spicy cod roe using kelp essential oil in the present invention.

상기 목적에 따라, 본 발명은 천연 항균성 물질로서 다시마 정유를 이용하여 품질 향상 및 저장성이 우수한 명란젓을 제조하기 위한 제조방법을 제공한다.In accordance with the above object, the present invention provides a manufacturing method for producing cod roe excellent in quality and shelf life using the kelp essential oil as a natural antimicrobial material.

구체적으로 본 발명은 명태로부터 명란을 채취하여 수세하는 선별 및 정선의 제 1단계; 상기 제 1단계의 명란에서 수분을 제거하고 절임물에 침지하여 1일 내지 4일, 바람직하게는 1일 내지 3일 동안 침지시키는 제 2단계; 상기 제 2단계의 침지된 명란에서 불순물 및 수분을 제거하는 제 3단계; 상기 제 3단계의 불순물이 제거된 명란에 조미하고 조미된 명란에 다시마 정유을 첨가한 마른양념을 바르는 제 4단계; 상기 마른 양념이 가미된 명란에 물양념을 바르는 제 5단계를 포함함을 특징으로 하는 제조공정으로부터 본 발명의 다시마 정유를 이용하여 품질향상 및 저장성이 우수한 명란젓을 수득할 수 있다. Specifically, the present invention comprises the first step of screening and selection to collect and wash water from pollack pollack; A second step of removing water from the first step and dipping in pickles to immerse for 1 to 4 days, preferably 1 to 3 days; A third step of removing impurities and water from the immersed light of the second step; A fourth step of applying dry seasoning to the seasoned cod roe and the seasoned cod roe with kelp essential oil; Using the kelp essential oil of the present invention from the manufacturing process characterized in that it comprises a fifth step of applying water seasoning to the dried seasoned cod roe, excellent cod roe can be obtained.

본 발명에서 사용되는 절임물은 명란 100 중량% 대비하여 소금 1 내지 20중량%, 설탕 1 내지 10중량%, 물 1 내지 10중량%, 및 청주 0.01 내지 5중량%, 바람직하게는 명란 100 중량% 대비하여 소금 1 내지 15중량%, 설탕 1 내지 10중량%, 물 1 내지 10중량%, 및 청주 0.01 내지 5중량%로 조성되어 있는 것을 특징으로 한다.The pickles used in the present invention are 1 to 20% by weight of salt, 1 to 10% by weight of sugar, 1 to 10% by weight of water, and 0.01 to 5% by weight of sake, preferably 100% by weight, based on 100% by weight of egg. It is characterized in that it is composed of 1 to 15% by weight of salt, 1 to 10% by weight of sugar, 1 to 10% by weight of water, and 0.01 to 5% by weight of sake.

본 발명에서 사용되는 명란 100 중량% 대비하여 고춧가루 1 내지 10중량%, 설탕 0.01 내지 5중량%, 및 다시마 정유 0.01% 내지 10%, 바람직하게는 명란 100 중량% 대비하여 고춧가루 1 내지 5중량%, 설탕 0.1 내지 3중량% 및 다시마 정유 0.1% 내지 6%로 조성되어 있는 것을 특징으로 한다.1 to 10% by weight of red pepper powder, 0.01 to 5% by weight of sugar, and 0.01% to 10% of kelp essential oil, preferably 1 to 5% by weight of red pepper powder, based on 100% by weight of cod roe, It is characterized in that it is composed of 0.1 to 3% by weight of sugar and 0.1% to 6% of kelp essential oil.

본 발명에서 사용되는 물양념은 명란 100 중량% 대비하여 고춧가루 1 내지 7중량%, 미원 0.01 내지 5중량%, 마늘 1 내지 10중량%, 물엿 1 내지 10중량%, 물 1 내지 15중량%, 바람직하게는 명란 100 중량% 대비하여 고춧가루 1 내지 5중량%, 미원 0.1 내지 3중량%, 마늘 2 내지 7중량%, 물엿 2 내지 7 중량%, 물 1내지 10중량%로 조성되어 있는 것을 특징으로 한다.Water seasoning used in the present invention is 1 to 7% by weight of red pepper powder, 0.01 to 5% by weight, 1 to 10% by weight of garlic, 1 to 10% by weight of starch syrup, 1 to 15% by weight, preferably 100% by weight It is characterized in that it is composed of 1 to 5% by weight of red pepper powder, 0.1 to 3% by weight, 2 to 7% by weight of garlic, 2 to 7% by weight of starch syrup, and 1 to 10% by weight of water, based on 100% by weight of cod roe. .

본원에서 정의되는 다시마 정유는 국내산 또는 수입산 다시마, 바람직하게는 국내산 다시마, 보다 바람직하게는 다시마류(Laminaria)속, 보다 더 바람직하게는 다시마(Laminaria jponica), 애기 다시마(Laminaria religiosa) 또는 개다시마 (Kjellmaniella crassifolia)를 건조 및 분쇄하여 절단하고, 상기 시료 총 중량의 약 1배 내지 200배(w/w), 바람직하게는 10배 내지 100배(w/w)의 정제수를 포함한 물, 주정, 탄소수 1 내지 4의 저급 알콜, 또는 이들의 혼합용매, 바람직하게는 물 또는 물 및 에탄올 혼합용매, 보다 바람직하게는 물 또는 50-99% 물 및 에탄올 혼합용매를 가하여 10분 내지 7일, 바람직하게는 20분 내지 2시간 동안, 0℃ 내지 150℃, 바람직하게는 10℃ 내지 100℃, 보다 바람직하게는 20℃ 내지 90℃에서 냉침추출, 열수추출, 초음파 추출, 환류냉각 추출 등의 추출방법, 바람직하게는, 초음파추출법을 수행하여 추출물을 수득하는 제 1단계; 상기에서 얻은 추출물을 여과하고 여과물을 감압 건조시키는 제 2단계의 제조공정을 통하여 본 발명의 다시마로부터 추출한 다시마 정유을 수득할 수 있다.
Kelp essential oils as defined herein are domestic or imported kelp, preferably domestic kelp, more preferably Laminaria, even more preferably Laminaria jponica, Laminaria religiosa or Gadamashima ( Kjellmaniella crassifolia) is dried and pulverized and cut, and includes water, spirits and carbon atoms containing about 1 to 200 times (w / w), preferably 10 to 100 times (w / w) purified water of the total weight of the sample. 1 to 4 lower alcohols, or mixed solvents thereof, preferably water or water and ethanol mixed solvents, more preferably water or 50-99% water and ethanol mixed solvents, for 10 minutes to 7 days, preferably For 20 minutes to 2 hours, extraction methods such as cold needle extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, and the like at 0 ° C. to 150 ° C., preferably 10 ° C. to 100 ° C., more preferably 20 ° C. to 90 ° C., are preferred. It has a first step of performing ultrasound extraction to give the extract; The kelp essential oil extracted from the kelp of the present invention may be obtained through the second step of manufacturing the filtered extract and drying the filtrate under reduced pressure.

이하, 본 발명의 명란젓의 제조방법을 상세히 설명한다.Hereinafter, the manufacturing method of the cod roe of the present invention will be described in detail.

제 1단계에서 북태평양 또는 동해에서 어획된 명태로부터 명란을 채취하여 수세하는 선별 및 정선을 수행하는 단계; Performing a selection and selection process of picking and washing the flag from the roasted seafood in the North Pacific or the East Sea in the first step;

제 2단계에서 상기 제 1단계의 명란에서 수분을 제거하고 절임물에 침지하여 1일 내지 4일, 바람직하게는 1일 내지 3일 동안 침지시키는 단계; Removing water from the first step of the egg in the second step and immersing in pickles to immerse for 1 to 4 days, preferably 1 to 3 days;

제 3단계에서 상기 제 2단계의 공정으로 처리한 명란은 불순물 및 수분을 제거시키는 단계; In the third step, the egg that has been treated in the process of the second step may include removing impurities and moisture;

제 4단계에서 상기 제 3단계의 불순물이 제거된 명란에 조미하고 조미된 명란에 마른양념을 바르는 단계; Applying seasoning to the seasoned cod roe and dried seasoned cod roe in a fourth step;

제 5단계에서 상기 마른 양념이 가미된 명란에 물양념을 바르는 제 5단계를 포함하는 제조공정으로부터 본 발명의 다시마 정유를 이용하여 품질 향상 및 저장성이 우수한 명란젓을 제조할 수 있다.Using the kelp essential oil of the present invention from the manufacturing process comprising the fifth step of applying the water seasoning to the dried seasoned cod roe in the fifth step can be produced excellent cod roe.

또한 본 발명은 상기 제조방법으로 수득되는 품질 향상 및 저장성이 우수한 다시마 정유를 첨가한 명란젓을 제공한다.
In another aspect, the present invention provides a spicy cod roe added with kelp essential oil excellent in quality improvement and shelf life obtained by the production method.

상기한 제조 공정으로 제조한 명란젓이 시중에서 판매되는 명란젓보다 미생물, 효모, 유산균, pH, VBN 및 TBA 측정결과, 품질 및 저장성이 탁월하여 최종적으로 변질로 인한 식량자원의 낭비를 막고, 제품의 생산 경비를 절감시킬 뿐만 아니라, 제품의 품질향상의 이점을 확인하였다.Cod roe prepared by the above manufacturing process is excellent in microbial, yeast, lactic acid bacteria, pH, VBN and TBA measurement results, superior quality and shelf life than commercially available cod roe to prevent waste of food resources due to deterioration. In addition to reducing costs, we have confirmed the benefits of product quality improvement.

본 발명은 천연 항균성 물질인 다시마 정유를 이용하여 본 발명의 공정으로 제조한 명란젓이 시중에서 판매되는 명란젓보다 미생물, 효모, 유산균, pH, VBN 및 TBA 측정결과, 품질 및 저장성이 탁월하여 최종적으로 변질로 인한 식량자원의 낭비를 막고, 제품의 생산 경비를 절감시킬 뿐만 아니라, 제품의 품질향상의 이점이 있기 때문에 식품학적 가치를 향상시키는데 기여할 수 있다.The present invention is a microbial, yeast, lactic acid bacteria, pH, VBN and TBA measurement results, quality and shelf life superior to the cod roe produced by the process of the present invention using the kelp essential oil, which is a natural antimicrobial substance, and finally deteriorated It not only prevents waste of food resources, reduces the production cost of the product, but also contributes to improving the food value because it has the advantage of improving the quality of the product.

이하, 본 발명을 실시예 및 실험예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

단, 하기 실시예 및 실험예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예 및 실험예에 의해 한정되는 것은 아니다.
However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples and experimental examples.

참고예Reference Example 1. 시료의 준비 1. Preparation of sample

명란은 속초에서 어획된 명태로부터 동결된 명란을 채취하여 사용하였고, 소금(천일염, 강릉시 수협), 설탕(백설탕, CJ), 청주(백화수복, 롯데주류), 미원(감칠맛나는 미원, 청정원), 물엿(옛날물엿, 오뚜기), 마늘(강릉시 하나로마트), 수돗물(강릉시), 고춧가루(해찬들고춧가루, CJ)을 구입 사용하였다.
The cod roe was harvested from frozen pollock caught in Sokcho and used as salt (sun salt, Gangneung-si Suhyup), sugar (white sugar, CJ), cheongju (baekhwa restoring, Lotte liquor), Miwon (flavorful beauty, clean water), Starch syrup (old starch syrup, Ottogi), garlic (Gangneung-si, Hanaro Mart), tap water (Gangneung-si), and red pepper powder (Haechan deul pepper, CJ) were used.

참고예Reference Example 2. 다시마 정유 제조 2. Kelp essential oil manufacture

다시마 정유로 사용한 다시마(Laminaria japonica)은 강릉시 수협에서 구입하여 사용하였으며, 분말화된 다시마(200 g 건조중량)에 95% ethanol 1,000 mL를 첨가 후 30℃에서 30분 동안 초음파추출기(Power Sonic520)로 초음파 추출법(sonication)을 수행한 후에 감압 여과하여 에탄올 추출물을 얻어 이를 감압농축기(rotary evaporator; Heating Bath B-490)로 40℃에서 용매를 제거한 후 -40℃이하에서 보관하면서 실험에 사용되었다.
Kelp ( Laminaria japonica ) used as essential oil for kelp was purchased from Gangneung-si Suhyup, and 1,000 mL of 95% ethanol was added to powdered kelp (200 g dry weight), followed by an ultrasonic extractor (Power Sonic520) for 30 minutes at 30 ° C. After performing sonication, the resultant was filtered under reduced pressure to obtain an ethanol extract, which was then used in an experiment while removing the solvent at 40 ° C. with a rotary evaporator (Heating Bath B-490) and storing it at −40 ° C. or lower.

실시예Example 1. 명란젓 제조  1. Cod roe manufacture

1-1. 다시마 정유를 첨가한 명란젓 제조1-1. Cod roe with kelp essential oil

상기 참고예 1의 냉동된 명란을 2일정도 상온에서 해동하여 세척하였다. 세척한 명란에 명란양 대비 소금 10%, 설탕 4%, 물 40%, 청주 0.5%의 간수로 48시간동안 염장을 하였다. 절인 명란을 세척, 선별 후 채반에서 3시간 동안 물기를 빼고, 명란양 대비 고춧가루 3%, 설탕 0.7%, 농도를 달리한 상기 다시마 정유를 각각 1% 또는 3% 첨가하여 마른양념을 하였다. 최종적으로 물양념을 명란양대비 고춧가루 2%, 미원 0.15%, 마늘 5%, 물엿 4.5%, 물 6.5%을 첨가하여 명란젓을 제조하여 멸균팩에 담아 25℃에 저장한 후에 하기 실험에 사용하였다. (이하, “다시마 정유 1% 및 3%”라고 각각 명명함).Frozen frozen egg of Reference Example 1 was washed by thawing at room temperature for about 2 days. The washed cod roe was salted for 48 hours with 10% salt, 4% sugar, 40% water, and 0.5% cheongju. After washing and screening the pickled cod roe, the water was drained for 3 hours from the rice tray, and dried seaweed was added by adding 1% or 3% of the kelp essential oil with varying concentrations of red pepper powder 3%, sugar 0.7% and different concentrations. Finally, the water seasoning was prepared by adding 2% red pepper powder, miwon 0.15%, garlic 5%, starch syrup 4.5%, water 6.5%, and putting it in a sterilized pack and storing it in a sterilization pack at 25 ° C. (Hereinafter referred to as “Dashima Oil Refinery 1% and 3%” respectively).

1-2. 1-2. 솔빈산을Solvin acid 첨가한 비교용 명란젓 제조 Preparation of Comparative Cod roe

상기 1-1 공정중 다시마 정유 대신에 시중에서 식품보존제로서 사용되는 솔빈산(S1626-250G, SIGMA)을 각각 1% 및 3%(중량%)를 대체하여 첨가하는 것만 제외하고는 상기 1-1 명란젓 제조공정과 동일한 방법을 이용하여 솔빈산을 첨가한 비교용 명란젓을 제조하였다(이하, “솔빈산 1% 및 3%”라고 각각 명명함).
1-1 except for adding sorbinic acid (S1626-250G, SIGMA), which is used as a food preservative, in place of 1% and 3% (wt%), respectively, instead of kelp essential oil during step 1-1. A comparative cod roe added with sorbic acid was prepared using the same method as the cod roe manufacturing process (hereinafter, referred to as “solvinic acid 1% and 3%”, respectively).

1-3. 1-3. 포도씨유를Grapeseed oil 첨가한 비교용 명란젓 제조 Preparation of Comparative Cod roe

상기 1-1 공정중 다시마 정유 대신에 시중에서 식품보존제로서 사용되는 포도씨유(오뚜기)을 각각 1% 및 3%(중량%)를 대체하여 첨가하는 것만 제외하고는 상기 1-1 명란적 제조공정과 동일한 방법을 이용하여 포도씨유을 첨가한 비교용 명란젓을 제조하였다(이하, 포도씨유 1% 및 3% 라고 각각 명명함).
1-1 coarse manufacturing process except that grape seed oil (OTTOGI), which is used as a food preservative instead of 1% and 3% (wt%), is added instead of kelp essential oil during 1-1. Comparative cod roe was added to the grape seed oil using the same method as described below (hereinafter referred to as grape seed oil 1% and 3%, respectively).

1-4. 1-4. 트레할로스를Trehalose 첨가한 비교용 명란젓 제조 Preparation of Comparative Cod roe

상기 1-1 공정중 다시마 정유 대신에 시중에서 식품보존제로서 사용되는 트레할로스(Trehalose, 제품번호PREHA, HAYASHYBARA) 을 각각 1% 및 3%(중량%)를 대체하여 첨가하는 것만 제외하고는 상기 1-1 명란적 제조공정과 동일한 방법을 이용하여 트레할로스을 첨가한 비교용 명란젓을 제조하였다(이하, Trehalose 1% 및 3% 라고 각각 명명함).
Except for adding 1% and 3% (wt%) of trehalose (Trehalose, product number PREHA, HAYASHYBARA), which is used as a food preservative in the market, instead of kelp essential oil during the process 1-1, A comparative cod roe with trehalose was prepared using the same method as in the 1 coarse preparation process (hereinafter, referred to as Trehalose 1% and 3%, respectively).

실험예Experimental Example 1. 다시마 정유 첨가 명란젓의 미생물 수 및  1. The number of microorganisms of cod roe refined with kelp essential oil pHpH 측정  Measure

1-1.미생물 수 측정1-1. Number of microorganisms

상기 실시예에서 제조된 명란젓에 대해 25(±5)℃에 3, 6, 9, 12일간 저장하면서 미생물 수를 하기의 방법을 이용하여 하기와 같이 측정하였다.The number of microorganisms was measured as follows using the following method while storing for 3, 6, 9 and 12 days at 25 (± 5) ℃ for the cod roe prepared in Example.

저장 중 명란젓의 미생물 총균수는 3M Aerobic Count Plate film(55144-1000), 유산균수는 BCP첨가 한천배지(DIFCO(E-MB31))를 사용하였다. The total number of microorganisms in the broth was 3M Aerobic Count Plate film (55144-1000) and the number of lactic acid bacteria was BCP-supplemented agar medium (DIFCO (E-MB31)).

상기 실험 결과, 다시마 정유를 활용한 고품질 명란젓의 숙성기간에 따른 총균수의 변화는 표 1와 같다. 다시마 정유 첨가 명란젓의 초기 총균수는 일반 명란젓의 총균수와 비교하여 크게 차이를 보이를 않았으나, 저장 3일째부터 9일째까지 총균수가 급격히 증가한 일반 명란젓에 비해 다시마 정유 첨가 명란젓은 저장기간 동안 초기 총균수와 비교하여 크게 차이를 보이지 않았다.As a result of the experiment, the total bacterial count according to the ripening period of the high-quality cod roe using kelp essential oil is shown in Table 1. The total number of spp. Cod fermented cod roe with kelp essential oil was not significantly different from that of cod cod roe, but compared with cod cod fermented with cod edible essential oil during the storage period. There was no significant difference compared to the number of bacteria.

저장중(25±0.5℃) 다시마 정유 첨가 명란젓의 총균수 변화 (단위: CFU/g)Changes in total bacterial counts of cod roe added with kelp essential oil during storage (25 ± 0.5 ℃) (unit: CFU / g) 시료sample 저장기간(일)Storage period (days) 00 33 66 99 ControlControl 7.1×105 7.1 x 10 5 1.1×107 1.1 × 10 7 1.9×109 1.9 × 10 9 2.2×109 2.2 x 10 9 솔빈산 1%Solvate 1% 6.6×105 6.6 × 10 5 6.5×105 6.5 × 10 5 5.7×105 5.7 × 10 5 7.4×108 7.4 × 10 8 솔빈산 3%Solvate 3% 6.4×105 6.4 × 10 5 4.6×105 4.6 × 10 5 4.7×105 4.7 × 10 5 5.3×108 5.3 × 10 8 포도씨유 1%1% grape seed oil 6.8×105 6.8 × 10 5 5.9×105 5.9 × 10 5 5.2×108 5.2 × 10 8 7.9×106 7.9 × 10 6 포도씨유 3%3% grape seed oil 8.5×105 8.5 × 10 5 3.8×106 3.8 × 10 6 9.6×108 9.6 × 10 8 1.6×109 1.6 × 10 9 Trehalose 1%Trehalose 1% 5.8×105 5.8 × 10 5 5.2×107 5.2 × 10 7 3.6×108 3.6 × 10 8 1.1×109 1.1 × 10 9 Trehalose 3%Trehalose 3% 4.8×105 4.8 × 10 5 1.2×106 1.2 × 10 6 4.2×106 4.2 × 10 6 1.2×107 1.2 × 10 7 다시마 정유 1%Kelp essential oil 1% 4.8×105 4.8 × 10 5 4.6×105 4.6 × 10 5 4.7×105 4.7 × 10 5 5.2×105 5.2 × 10 5 다시마 정유 3%Kelp essential oil 3% 3.6×105 3.6 × 10 5 4.7×105 4.7 × 10 5 9.0×105 9.0 × 10 5 4.7×105 4.7 × 10 5

유산균수Lactobacillus 측정 Measure

다시마 정유를 활용한 고품질 명란젓의 숙성기간에 따른 유산균수의 변화는 표 2와 같다. 저장 6일째 일반 명란젓에 비해 다시마 정유 첨가 명란젓의 유산균수가 높게 나타났으며, 저장 9일째는 약 10배 이상으로 높게 나타났다. 총균수는 일반 명란젓이 높았으나, 다시마 정유 첨가 명란젓의 유산균수가 상대적으로 높게 나타난 결과를 바탕으로 다시마 정유를 첨가가 명란젓중의 유산균수 상승이라는 발효제품 품질 지수를 향상시킴을 확인하였다.The changes in the number of lactic acid bacteria according to the ripening period of high-quality cod roe using kelp essential oil are shown in Table 2. On the 6th day of storage, the number of lactic acid bacteria of keratin added with kelp essential oil was higher than that of normal cod roe, and on the 9th day of storage, it was about 10 times higher. The total number of bacteria was higher in general cod roe, but based on the results of the relatively high lactic acid bacteria of cod roe, the addition of kelp essential oil improved the fermentation product quality index.

저장중(25±0.5℃) 다시마 정유 첨가 명란젓의 유산균수 변화 (단위: CFU/g)Changes in Lactic Acid Bacteria in Cod Cod with Tangle Essential Oil during Storage (25 ± 0.5 ℃) (Unit: CFU / g) 시료sample 저장기간(일)Storage period (days) 66 99 ControlControl 3.4×104 3.4 × 10 4 5.7×104 5.7 × 10 4 다시마 정유 1%Kelp essential oil 1% 6.5×105 6.5 × 10 5 5.7×105 5.7 × 10 5 다시마 정유 3%Kelp essential oil 3% 3.5×106 3.5 × 10 6 4.6×105 4.6 × 10 5

1-2. 1-2. pHpH 측정 Measure

상기 실시예에서 제조된 명란젓에 대해 25(±5)℃에 3, 6, 9, 12일간 저장하면서 pH를 하기의 방법을 이용하여 측정하였다.The pH of the cod roe prepared in Example was stored at 25 (± 5) ° C. for 3, 6, 9, 12 days, and measured using the following method.

pH 측정은 시료 10 g을 취해서 증류수 20 mL를 가해 균질화 시킨 후 pH meter(Mettler Toledo, SevenEasy pH, switzerland)로 측정하였다.
The pH was measured with a pH meter (Mettler Toledo, SevenEasy pH, Switzerland) after taking 10 g of sample and homogenizing with 20 mL of distilled water.

상기 실험 결과, 다시마 정유를 활용한 고품질 명란젓의 숙성기간에 따른 pH 변화는 표 3과 같다. 초기 명란젓의 pH는 4.7-6.1 로 시료간에 유의적 차이를 보였다. 일반 명란젓은 저장 12일까지 지속적으로 pH가 증가한 반면에, 항산화제 및 항균제 첨가 명란젓과 다시마 정유 첨가 명란젓은 pH 변화가 없거나 감소하였다.As a result of the experiment, the pH change according to the ripening period of high-quality cod roe using kelp essential oil is shown in Table 3. The pH of the early cod roe was 4.7-6.1, which showed a significant difference between samples. General cod roe increased up to 12 days of storage, whereas cod roe with antioxidants and antimicrobial agents and cod roe with kelp essential oil showed no or decreased pH.

저장중(25±0.5℃) 다시마 정유 첨가 명란젓의 pH 변화Changes in pH of cod roe with kelp essential oil during storage (25 ± 0.5 ℃) 시료sample 저장기간(일)Storage period (days) 00 33 66 99 1212 ControlControl 6.0±0.0bc 6.0 ± 0.0 bc 6.0±0.0c 6.0 ± 0.0 c 6.0±0.0b 6.0 ± 0.0 b 6.3±0.1a 6.3 ± 0.1 a 6.1±0.0b 6.1 ± 0.0 b 솔빈산 1%Solvate 1% 5.2±0.0d 5.2 ± 0.0 d 5.1±0.0f 5.1 ± 0.0 f 5.0±0.0h 5.0 ± 0.0 h 5.0±0.0h 5.0 ± 0.0 h 5.0±0.0f 5.0 ± 0.0 f 솔빈산 3%Solvate 3% 4.7±0.0e 4.7 ± 0.0 e 4.9±0.0g 4.9 ± 0.0 g 4.9±0.0j 4.9 ± 0.0 j 4.8±0.0i 4.8 ± 0.0 i 4.7±0.0g 4.7 ± 0.0 g 포도씨유 1%1% grape seed oil 6.0±0.0ab 6.0 ± 0.0 ab 6.0±0.0d 6.0 ± 0.0 d 5.8±0.0e 5.8 ± 0.0 e 5.9±0.0d 5.9 ± 0.0 d 6.2±0.0a 6.2 ± 0.0 a 포도씨유 3%3% grape seed oil 6.0±0.0bc 6.0 ± 0.0 bc 6.0±0.0c 6.0 ± 0.0 c 6.1±0.0a 6.1 ± 0.0 a 6.2±0.0b 6.2 ± 0.0 b 6.2±0.0a 6.2 ± 0.0 a Trehalose 1%Trehalose 1% 6.0±0.0bc 6.0 ± 0.0 bc 5.9±0.0e 5.9 ± 0.0 e 5.9±0.0c 5.9 ± 0.0 c 6.0±0.0c 6.0 ± 0.0 c 6.2±0.0a 6.2 ± 0.0 a Trehalose 3%Trehalose 3% 6.1±0.0ab 6.1 ± 0.0 ab 5.9±0.0e 5.9 ± 0.0 e 5.8±0.0f 5.8 ± 0.0 f 5.6±0.0g 5.6 ± 0.0 g 5.5±0.0e 5.5 ± 0.0 e 다시마 정유 1%Kelp essential oil 1% 6.1±0.0a 6.1 ± 0.0 a 6.1±0.0a 6.1 ± 0.0 a 5.9±0.0d 5.9 ± 0.0 d 5.8±0.0e 5.8 ± 0.0 e 5.9±0.0c 5.9 ± 0.0 c 다시마 정유 3%Kelp essential oil 3% 6.1±0.1c 6.1 ± 0.1 c 6.1±0.2b 6.1 ± 0.2 b 5.8±0.0g 5.8 ± 0.0 g 5.7±0.0f 5.7 ± 0.0 f 5.8±0.0d 5.8 ± 0.0 d Values are mean±SD (n=3).
a- kValues with the same letter are not significantly different(p<0.05).
Values are mean ± SD (n = 3).
a- k Values with the same letter are not significantly different (p <0.05).

실험예Experimental Example 2. 신선도( 2. Freshness ( VBNVBN , 휘발성 염기 질소) 측정 , Volatile base nitrogen) measurement

상기 실시예 1에서 제조된 명란젓에 대해 25(±5)℃에 3, 6, 9, 12일간 저장하면서 하기와 같이 신선도를 측정하였다.Freshness was measured as described below while storing the cod roe prepared in Example 1 at 25 (占 5) 占 폚 for 3, 6, 9, and 12 days.

시료 2 g을 취해 증류수 16 mL를 가하고 20% TCA용액 2 mL를 넣고 잘 마쇄한 다음 10분간 방치한 후 여과하여 상징액을 취한 후 Conway unit를 이용한 미량 확산법으로 휘발성 염기질소의 양을 구하였다. Take 2 g of sample, add 16 mL of distilled water, add 2 mL of 20% TCA solution, grind well, leave for 10 minutes, filter, collect supernatant, and quantify volatile basic nitrogen by microdiffusion method using Conway unit.

다시마 정유를 활용한 고품질 명란젓의 숙성기간에 따른 VBN 변화는 표 4와 같다. 초기 VBN함량은 19.3-23.1 mg% 이였으며 일반 명란젓에 비해 다시마 정유 첨가 명란젓과 포도씨유, Trehalose 첨가 명란젓의 VBN함량이 높게 나타났다. 저장 3일째부터는 다시마 정유 첨가 명란젓이 일반 명란젓뿐만 아니라 솔빈산, 포도씨유, Trehalose 첨가 명란젓보다 VBN함량이 낮게 나타났으며, 저장 마지막 날까지 41.6-220.4 mg%로 유의적인 차이를 보였다. 이 결과, 다시마 정유 첨가로 인해 명란젓의 부패를 크게 억제한 것을 확인하였으며, 솔빈산, 포도씨유, Trehalose와 같은 항산화제와 항균제보다 효과가 좋음을 확인하였다.VBN changes according to the maturation period of high-quality cod roe using kelp essential oil are shown in Table 4. The initial VBN content was 19.3-23.1 mg%, and the VBN content of spicy cod roe, grapeseed oil and trehalose cod roe was higher than that of cod roe. From the 3rd day of storage, VBN content was lower than that of solvin acid, grapeseed oil and Trehalose as well as general cod roe, but it was significantly different (41.6-220.4 mg%). As a result, it was confirmed that the addition of kelp essential oil significantly inhibited the corruption of cod roe, and it was confirmed that it was more effective than antioxidants and antimicrobial agents such as sorbic acid, grape seed oil, and Trehalose.

저장중(25±0.5℃) 다시마 정유 첨가 명란젓의 VBN , 변화 (단위: mg%) VBN , Changes of Cod roe with Sea Tangle Essential Oil during Storage (25 ± 0.5 ℃) (Unit: mg%) 시료sample 저장기간(일)Storage period (days) 00 33 66 99 1212 ControlControl 19.8±1.3bc 19.8 ± 1.3 bc 44.8±2.1a 44.8 ± 2.1 a 115.9±18.7a 115.9 ± 18.7 a 156.9±5.2b 156.9 ± 5.2 b 197.9±2.0b 197.9 ± 2.0 b 솔빈산 1%Solvate 1% 19.3±0.0c 19.3 ± 0.0 c 44.9±0.5a 44.9 ± 0.5 a 50.6±0.9d 50.6 ± 0.9 d 52.1±1.4d 52.1 ± 1.4 d 66.6±1.6e 66.6 ± 1.6 e 솔빈산 3%Solvate 3% 19.6±1.1bc 19.6 ± 1.1 bc 42.1±1.6b 42.1 ± 1.6 b 43.5±1.6de 43.5 ± 1.6 de 50.5±1.2d 50.5 ± 1.2 d 68.6±0.6e 68.6 ± 0.6 e 포도씨유 1%1% grape seed oil 21.4±1.0abc 21.4 ± 1.0 abc 40.9±1.1b 40.9 ± 1.1 b 54.6±2.7d 54.6 ± 2.7 d 111.9±0.9c 111.9 ± 0.9 c 198.7±0.8b 198.7 ± 0.8 b 포도씨유 3%3% grape seed oil 21.8±0.7abc 21.8 ± 0.7 abc 29.2±1.0d 29.2 ± 1.0 d 66.0±4.6c 66.0 ± 4.6 c 155.6±1.8b 155.6 ± 1.8 b 188.4±8.0c 188.4 ± 8.0 c Trehalose 1%Trehalose 1% 22.8±2.2a 22.8 ± 2.2 a 32.0±0.3c 32.0 ± 0.3 c 88.6±0.6b 88.6 ± 0.6 b 177.0±4.7a 177.0 ± 4.7 a 220.4±4.1a 220.4 ± 4.1 a Trehalose 3%Trehalose 3% 23.1±0.3a 23.1 ± 0.3 a 31.8±0.7c 31.8 ± 0.7 c 86.5±1.6b 86.5 ± 1.6 b 113.9±3.0c 113.9 ± 3.0 c 169.4±5.4d 169.4 ± 5.4 d 다시마 정유 1%Kelp essential oil 1% 22.1±1.1ab 22.1 ± 1.1 ab 23.8±0.7e 23.8 ± 0.7 e 34.3±1.0e 34.3 ± 1.0 e 37.6±1.3f 37.6 ± 1.3 f 41.6±0.6f 41.6 ± 0.6 f 다시마 정유 3%Kelp essential oil 3% 22.7±2.3a 22.7 ± 2.3 a 26.6±1.1f 26.6 ± 1.1 f 37.1±1.6e 37.1 ± 1.6 e 44.4±2.1e 44.4 ± 2.1 e 45.3±0.9f 45.3 ± 0.9 f Values are mean±SD (n=3).
a- hValues with the same letter are not significantly different(p<0.05).
Values are mean ± SD (n = 3).
a- h Values with the same letter are not significantly different (p <0.05).

실험예 3. 항산화능 (Thiobarbituric acid(TBA); 지질산화) 측정 Experimental Example 3. Measurement of Thiobarbituric acid (TBA)

상기 실시예 1에서 제조된 명란젓에 대해 25(±5)℃에 3, 6, 9, 12일간 저장하면서 하기와 같이 항산화능을 측정하였다.The antioxidant activity of the cauliflower prepared in Example 1 was measured at 25 (占 5) 占 폚 for 3, 6, 9 and 12 days as follows.

마쇄한 명란젓 20g을 취하여 4℃로 냉각된 2M 인산이 포함된 20% 트리클로로아세트산(20% Trichloroacetic acid in 2M phosphoric acid)용액 50mL를 1.5분간 균질하게 마쇄시킨 후 마쇄액을 100mL 정용 플라스크로 정용하였다. 이 액 50mL를 와트만(WhaTBAn No. 1) 여과지로 여과한 후 원심분리기(미량원심분리기; Mega 21R) 15000ppm에서 10분간 돌린 후 상층액만 5mL 취해 실린지로 여과하여 시험관에 옮긴 후 0.005M 2-TBA(thiobarbituric acid) 5mL를 넣었다. 발색된 액을 530nm에서 흡광도(Jasco, JP/V-550)를 측정하여 측정된 흡광도에 5.2를 곱한 수치로 하였다.20 g of the shredded cod roe was poured into 50 mL of 20% trichloroacetic acid in 2M phosphoric acid solution containing 2 M phosphoric acid cooled at 4 ° C for 1.5 minutes, and the resulting solution was used in a 100 mL constant volume flask . After filtrating 50 mL of this solution with a filter of WhaTBAn No. 1, the solution was turned for 10 minutes at 15000 ppm of a centrifuge (Micro Centrifuge; Mega 21R), and only 5 mL of the supernatant was filtered through a syringe, and then transferred to a test tube. 5 mL of thiobarbituric acid (TBA) was added thereto. The color developed liquid was measured at 530 nm absorbance (Jasco, JP / V-550), and the measured absorbance was multiplied by 5.2.

다시마 정유를 활용한 고품질 명란젓의 숙성기간에 따른 TBA 변화는 표 5과 같다. 저장 9일째까지 다시마 정유 첨가에 따른 명란젓의 항산화효과는 확인 할 수 없었으나 저장 12일째부터 다시마 정유 첨가 명란젓이 일반 명란젓에 비해 TBA값이 유의적으로 낮았다. 이 결과로 다시마 정유가 명란젓의 산화를 억제하는 것을 확인하였다.The TBA changes according to the maturation period of high-quality cod roe using kelp essential oil are shown in Table 5. Antioxidant effect of cod roe was not confirmed by the addition of kelp essential oil until 9th day of storage. As a result, it was confirmed that kelp essential oil inhibits oxidation of cod roe.

저장중(25±0.5℃) 다시마 정유 첨가 명란젓의 TBA변화 (단위: O.D)Change in TBA of Cod roe with Sea Tangle Essential Oil during Storage (25 ± 0.5 ℃) (Unit: OD) 시료sample 저장기간(일)Storage period (days) 00 33 66 99 1212 ControlControl 0.1±0.0e 0.1 ± 0.0 e 0.1±0.0cd 0.1 ± 0.0 cd 0.1±0.0c 0.1 ± 0.0 c 0.1±0.0e 0.1 ± 0.0 e 0.6±0.2ab 0.6 ± 0.2 ab 솔빈산 1%Solvate 1% 0.1±0.0cd 0.1 ± 0.0 cd 0.2±0.0b 0.2 ± 0.0 b 0.3±0.0a 0.3 ± 0.0 a 0.4±0.0a 0.4 ± 0.0 a 0.5±0.0abc 0.5 ± 0.0 abc 솔빈산 3%Solvate 3% 0.1±0.0bc 0.1 ± 0.0 bc 0.1±0.0cd 0.1 ± 0.0 cd 0.3±0.0a 0.3 ± 0.0 a 0.4±0.0a 0.4 ± 0.0 a 0.5±0.0abc 0.5 ± 0.0 abc 포도씨유 1%1% grape seed oil 0.1±0.0de 0.1 ± 0.0 de 0.2±0.0a 0.2 ± 0.0 a 0.1±0.0c 0.1 ± 0.0 c 0.2±0.0b 0.2 ± 0.0 b 0.5±0.0abc 0.5 ± 0.0 abc 포도씨유 3%3% grape seed oil 0.1±0.0de 0.1 ± 0.0 de 0.2±0.0a 0.2 ± 0.0 a 0.2±0.0b 0.2 ± 0.0 b 0.2±0.0c 0.2 ± 0.0 c 0.4±0.0c 0.4 ± 0.0 c Trehalose 1%Trehalose 1% 0.1±0.0de 0.1 ± 0.0 de 0.1±0.0d 0.1 ± 0.0 d 0.1±0.0cd 0.1 ± 0.0 cd 0.2±0.0c 0.2 ± 0.0 c 0.5±0.1abc 0.5 ± 0.1 abc Trehalose 3%Trehalose 3% 0.1±0.0cde 0.1 ± 0.0 cde 0.1±0.0d 0.1 ± 0.0 d 0.1±0.0c 0.1 ± 0.0 c 0.2±0.0d 0.2 ± 0.0 d 0.6±0.1c 0.6 ± 0.1 c 다시마 정유 1%Kelp essential oil 1% 0.2±0.0a 0.2 ± 0.0 a 0.1±0.0c 0.1 ± 0.0 c 0.1±0.0c 0.1 ± 0.0 c 0.2±0.0d 0.2 ± 0.0 d 0.4±0.0bc 0.4 ± 0.0 bc 다시마 정유 3%Kelp essential oil 3% 0.2±0.0ab 0.2 ± 0.0 ab 0.1±0.0cd 0.1 ± 0.0 cd 0.1±0.0d 0.1 ± 0.0 d 0.1±0.0f 0.1 ± 0.0 f 0.4±0.0c 0.4 ± 0.0 c Values are mean±SD (n=3).
a- eValues with the same letter are not significantly different(p<0.05).
Values are mean ± SD (n = 3).
a- e Values with the same letter are not significantly different (p <0.05).

Claims (7)

명태로부터 명란을 채취하여 수세하는 선별 및 정선의 제 1단계; 상기 제 1단계의 명란에서 수분을 제거하고 절임물에 침지하여 1일 내지 4일 동안 침지시키는 제 2단계; 상기 제 2단계의 침지된 명란에서 불순물 및 수분을 제거하는 제 3단계; 상기 제 3단계의 불순물이 제거된 명란에 조미하고 조미된 명란에 다시마 정유를 첨가한 마른양념을 바르는 제 4단계; 상기 마른 양념이 가미된 명란에 물양념을 바르는 제 5단계를 포함함을 특징으로 하는 다시마 정유를 첨가한 명란젓의 제조방법.The first step of selection and selection of pickling and pickling from pollack; A second step of removing water from the first step and dipping in pickles to immerse for 1 to 4 days; A third step of removing impurities and water from the immersed light of the second step; A fourth step of applying a dry seasoning to the seasoned cod roe and the seasoned cod roe with kelp essential oil; A method of manufacturing spicy cod roe with kelp essential oil, characterized in that it comprises a fifth step of applying water seasoning to the dried seasoned cod roe. 제 1항에 있어서, 상기 절임물은 명란 100 중량% 대비하여 소금 1 내지 20중량%, 설탕 1 내지 10중량%, 물 1 내지 10중량%, 및 청주 0.01 내지 5중량%로 조성되어 있는 것을 특징으로 하는 제조방법.The method of claim 1, wherein the pickles are 1 to 20% by weight of salt, 1 to 10% by weight of sugar, 1 to 10% by weight of water, and 0.01 to 5% by weight of sake based on 100% by weight of egg. The manufacturing method to make. 제 1항에 있어서, 상기 마른양념은 명란 100 중량% 대비하여 고춧가루 1 내지 10중량%, 설탕 0.01 내지 5중량% 및 다시마 정유 0.01% 내지 10%로 조성되어 있는 것을 특징으로 하는 제조방법.The method of claim 1, wherein the dried seasoning is 1 to 10% by weight of red pepper powder, 0.01 to 5% by weight sugar and 0.01% to 10% kelp essential oil compared to 100% by weight of the egg. 제 1항에 있어서, 상기 물양념은 명란 100 중량% 대비하여 고춧가루 1 내지 7중량%, 미원 0.01 내지 5중량%, 마늘 1 내지 10중량%, 물엿 1 내지 10중량%, 물 1 내지 15중량%로 조성되어 있는 것을 특징으로 하는 제조방법.According to claim 1, wherein the water seasoning is 1 to 7% by weight of red pepper powder, 0.01 to 5% by weight, 1 to 10% by weight of garlic, 1 to 10% by weight of starch syrup, 1 to 15% by weight, based on 100% by weight of egg Manufacturing method characterized in that the composition. 제 1항에 있어서, 상기 다시마 정유는 국내산 또는 수입산 다시마로부터 추출된 다시마 정유임을 특징으로 하는 제조방법.The method of claim 1, wherein the kelp essential oil is kelp essential oil extracted from domestic or imported kelp. 제 5항에 있어서, 상기 다시마 정유는 다시마(Laminaria spp.)을 건조 및 분쇄하여 절단하고, 상기 시료 총 중량의 1배 내지 200배(w/w)의 정제수를 포함한 물, 주정, 탄소수 1 내지 4의 저급 알콜, 또는 이들의 혼합용매를 가하여 10분 내지 7일 동안, 0℃ 내지 150℃에서 냉침추출, 열수추출, 초음파 추출, 또는 환류냉각 추출방법을 수행하여 추출물을 수득하는 제 1단계; 상기에서 얻은 추출물을 여과하고 여과물을 감압 건조시키는 제 2단계의 제조공정을 통하여 수득된 다시마 정유임을 특징으로 하는 제조방법.The kelp essential oil according to claim 5, wherein the kelp essential oil is cut by drying and grinding kelp (Laminaria spp.) And containing 1 to 200 times (w / w) purified water of the total weight of the sample. Adding a lower alcohol of 4 or a mixed solvent thereof to obtain an extract by performing cold extraction, hot water extraction, ultrasonic extraction, or reflux cooling extraction method at 0 ° C. to 150 ° C. for 10 minutes to 7 days; The method of claim 1, wherein the extract is filtered and the filtrate is dried under reduced pressure. 제 1항의 제조방법으로 수득되는 품질 향상 및 저장성이 우수한 다시마 정유를 첨가한 명란젓.Cod roe added with kelp essential oil excellent in quality improvement and shelf life obtained by the method of claim 1.
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