KR101330451B1 - A method for preparing the salted Alaska pollack roe using by Hizikia fusifirmia oil - Google Patents

A method for preparing the salted Alaska pollack roe using by Hizikia fusifirmia oil Download PDF

Info

Publication number
KR101330451B1
KR101330451B1 KR1020120013438A KR20120013438A KR101330451B1 KR 101330451 B1 KR101330451 B1 KR 101330451B1 KR 1020120013438 A KR1020120013438 A KR 1020120013438A KR 20120013438 A KR20120013438 A KR 20120013438A KR 101330451 B1 KR101330451 B1 KR 101330451B1
Authority
KR
South Korea
Prior art keywords
weight
parts
cod roe
oil
essential oil
Prior art date
Application number
KR1020120013438A
Other languages
Korean (ko)
Other versions
KR20130092012A (en
Inventor
조순영
라경민
최성미
최지연
임미진
조현덕
김도윤
최윤정
Original Assignee
강릉원주대학교산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 강릉원주대학교산학협력단 filed Critical 강릉원주대학교산학협력단
Priority to KR1020120013438A priority Critical patent/KR101330451B1/en
Publication of KR20130092012A publication Critical patent/KR20130092012A/en
Application granted granted Critical
Publication of KR101330451B1 publication Critical patent/KR101330451B1/en

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/30Fish eggs, e.g. caviar; Fish-egg substitutes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/02Preserving by means of inorganic salts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings, cooking oils
    • A23D9/02Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/65Addition of, or treatment with, microorganisms or enzymes
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00Production of fats or fatty oils from raw materials
    • C11B1/10Production of fats or fatty oils from raw materials by extracting

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Meat, Egg Or Seafood Products (AREA)

Abstract

본 발명은 톳 정유를 이용하여 명란젓을 제조하기 위한 제조방법에 관한 것으로, 상세하게는 천연 항균성 물질로서 톳 정유를 이용하여 본 발명의 공정으로 제조한 명란젓이 시중에서 판매되는 명란젓보다 미생물, 효모, 유산균, pH, VBN 및 TBA 측정결과 저장성 및 저장성이 탁월하여 최종적으로 변질로 인한 식량자원의 낭비를 막고, 제품의 생산 경비를 절감시킬 뿐만 아니라, 제품의 위생적 품질향상의 이점이 있어 우수한 품질의 명란젓을 제조할 수 있다.The present invention relates to a manufacturing method for producing cod roe using 톳 essential oil, and more specifically, the cod roe produced by the process of the present invention using 톳 essential oil as a natural antimicrobial substance, microorganisms, yeast, Lactobacillus, pH, VBN and TBA results in excellent storage and storage to prevent waste of food resources due to deterioration, reduce production cost of products, and improve the hygienic quality of products. Can be prepared.

Description

톳 정유를 이용한 명란젓의 제조방법 {A method for preparing the salted Alaska pollack roe using by Hizikia fusifirmia oil}A A method for preparing the salted Alaska pollack roe using by Hizikia fusifirmia oil}

본 발명은 천연 항균성 물질로서 톳 정유를 이용하여 품질향상 및 저장성이 우수한 명란젓을 제조하는 방법에 관한 것이다.The present invention relates to a method for producing cod roe excellent in quality improvement and shelf life by using essential oils as natural antimicrobial substances.

[문헌 1] 김상무, 식품첨가제에 의한 저염 명란젓의 보존 효과, 한국식품영양과학회지, 25 (6), pp.937-943, 1996 [1] Kim, Sang Moo, Conservation effect of low salt cod roe by food additives, Korean Journal of Food Science and Nutrition, 25 (6) , pp.937-943, 1996

[문헌 2] 우원식. 2005. 천연물화학연구법. 서울대학교출판부. 167-235.[Document 2] Woo Wonsik. 2005. Natural Products Chemistry Research Act. Seoul National University Press. 167-235.

[문헌 3] 최한길외, 해양식물과 한의학, (주)학술정보, pp.18, 175-176, 2004[Reference 3] Choi, Han-Gil et al., Marine Plants and Oriental Medicine, Academic Information, pp.18, 175-176, 2004

젓갈은 어패류에 식염을 가하여 염장함으로써 부패균의 번식을 억제하고 자가소화효소 또는 미생물의 효소작용에 의해 육질을 분해시킨 우리나라 전통의 수산발효식품으로서 제조 공정이 단순하고 특별한 제조장치도 필요하지 않으며 저장후의 제품은 독특한 감칠맛을 가지고 있어 예부터 오늘에 이르기까지 밑반찬이나 톳치의 조미 소재로 많이 이용되고 있다(톳상무, 식품첨가제에 의한 저염 명란젓의 보존 효과, 한국식품영양과학회지, 25 (6), pp.937-943, 1996). Salted seafood is a traditional Korean fermented fish food that suppresses the growth of decayed bacteria by salting fish and shellfish and decomposes meat by the enzyme action of autodigestive enzyme or microorganism.The manufacturing process is simple and no special manufacturing equipment is needed. The product has a unique umami, and has been used a lot as a seasoning material for side dishes and squid from ancient times until now. (Preservation effect of low salted cod roe with food additives, food additives, Korean Journal of Food Science and Nutrition, 25 (6) , pp.937-943, 1996).

최근들어 건강 지향적인 식품의 소비가 증가함에 따라 젓갈제품은 염의 함량이 훨씬 낮은 양념 젓갈의 형태로 많이 이용되고 있는 실정이나, 이러한 저식염의 양념젓갈은 제조시에 발생되는 가장 큰 문제점 중의 하나가 젓갈의 저장성의 단축이다. 이에 저염 젓갈의 저장성을 연장하려는 연구가 폭넓게 이루어지고 있으나 아직까지 뚜렷한 해결방안이 마련되어 있지 않다. In recent years, as consumption of health-oriented foods has increased, the fermented product has been widely used in the form of seasoned salted fish which has a much lower salt content. However, such seasoned salted salted fish is one of the biggest problems It is shortening of storage stability of salted fish. To this end, researches to extend the shelf life of low salted salted fish have been widely conducted, but there are no clear solutions yet.

식물 정유는 특정식물체를 증류 또는 압착하여 얻어지는 휘발성의 향기성분으로서, 식물, 동물, 미생물들에서 방어기작, 신호전달, 2차대사를 위하여 생합성된 화학물질의 혼합물이다(우원식. 2005. 천연물화학연구법. 서울대학교출판부. 167-235)Plant essential oils are volatile fragrances obtained by distilling or compressing certain plants and are mixtures of chemicals synthesized for defense mechanisms, signaling and secondary metabolism in plants, animals and microorganisms (Woo Won Sik. 2005. Natural Products Chemistry Research Method) Seoul National University Press, 167-235)

특히, 톳류(Sea weed fusiforme; Hizikia fusifirmia)는 갈조식물 모자반과의 바닷말. 조간대 하부에 서식하며 유성세대만 존재하는 다년생 해조류이다. 맛이 좋아 식용으로 이용되며 특히 칼슘과 철분을 풍부하게 함유하며마산·진해·창원·거제에서는 ‘톳나물’이라 하며, 고창에서는 '따시래기', '흙배기'라고 부른다. 제주지역에서는 ‘톨’이라 하며 보릿고개가 존재했을 당시 톳밥 등을 지어 구황식품으로 이용하기도 하였다. 사슴꼬리와 유사하다고 하여 '녹미채(鹿尾菜)'라 부르기도 한다. 조간대 하부에서 경사가 완만한 암초지대나 파도가 심하지 않으며 뻘이 약간 덮인 지역에 큰 군락을 이룬다. 섬유상의 뿌리를 가지고 있으며 줄기는 원뿔형이다. 잎은 주걱모양으로 하부에서만 볼 수 있고 다육질이며 작고 가장자리에 톱니가 있다. 유성세대만 있는 다년생 해조류 유성생식과 영양번식을 한다. 4∼5월에 생식기관이 형성되고 기부만 남긴 후 유실(流失)된다. 이 식물은 칼슘·요오드·철 등의 무기염류가 많이 포함되어 있어, 혈관경화를 막아 주고, 상용으로 먹으면 치아가 건강해지며 머리털이 윤택해지고, 임신부인 경우에는 태아의 뼈를 튼튼하게 해주는 등 예로부터 기호식품의 하나로서 특히 일본 사람들이 잘 먹는다. 섬유질을 포함하고 있어 변비에도 좋으며, 점액질의 물질이 창자의 소화운동을 높여 준다. 자연종묘에 의존하고 있었으나 최근 한국의 남해안에서 자연산을 채취하여 이식양식(移植養殖)을 시도하고 있는데, 그것은 아직도 포자에 의한 양식법이 개발되지 못하고 있기 때문이다. 한국에서는 주문진 이남에서 서해안 장산곶까지 생육하고 남해안과 제주에서 잘 자란다. 톳밥이나, 무침, 샐러드, 냉국 등으로 요리된다. 봄에서 초여름에 가장 연하고 맛이 좋다. ([출처] 톳 [Sea weed fusiforme ] 네이버 백과사전, www.naver.com), 일반성분으로는 지질, 단백질, 섬유질, 당질, 화분 등을 함유한 것으로 알려져 있다 (최한길외, 해양식물과 한의학, (주)학술정보, pp.64-65, 2004).In particular, the sea weed fusiforme (Hizikia fusifirmia) is a sea horse with brown algae. It is a perennial seaweed that lives in the lower intertidal zone and has only a meteor generation. It tastes good and is used for food. It contains abundant calcium and iron, and it is called 'namulmul' in Masan, Jinhae, Changwon, and Geoje. In the Jeju area, it was called 'tol' and was used as a food for wangbap when the barley hill existed. Similar to deer tail, it is also called 'Nomichae (鹿 尾 菜)'. In the lower intertidal zone, it forms a large colony in a gentle reef area or a modestly covered area with small waves. It has fibrous roots and the stem is conical. Leaves are spatula, visible only at the bottom, fleshy, small, and serrated at the edges. Perennial algae reproductive and nutrient breeding with only the sexual generation. Reproductive organs are formed in April-May, and only the basal leaves are lost. This plant contains a lot of inorganic salts such as calcium, iodine, iron, etc. It prevents vascular hardening, and when it is eaten commercially, teeth are healthy, hair is moisturized, and in the case of pregnant women, the fetus bones are strengthened. One of the favorite foods, especially Japanese people eat well. Containing fiber is also good for constipation, mucous substances increase the digestive movement of the intestines. Although it was dependent on natural seedlings, it has recently tried to transplant cultures by harvesting wild products from the south coast of Korea, because the farming method by spores is still not developed. In Korea, it grows from south of Jumunjin to Jangsan Cape on the west coast and grows well on the south coast and Jeju. It is cooked with rice, radish, salad and cold soup. It is the lightest and most delicious in spring to early summer. ([Source] 톳 [Sea weed fusiforme] Naver Encyclopedia, www.naver.com ), Its general ingredients are known to contain lipids, proteins, fiber, sugars, pollen, etc. Academic Information, pp.64-65, 2004).

이에 따라 본 발명자들은 젓갈의 맛과 질을 향상시킬 수 있는 방법에 관하여 연구하던 중 젓갈 변질 원인균의 증식을 억제시킬 것이라 기대되는 천연 항균성 물질로서 톳 정유를 이용하여 제조한 명란젓이 시중에서 판매되는 명란젓보다 미생물, pH, 아미노태질소, VBN 및 TBA 측정결과, 저장성이 탁월함에 따라 변질로 인한 반품 및 폐기되는 양을 줄여 식량자원의 낭비를 막고, 제품의 생산 경비를 절감시킬 뿐만 아니라, 제품의 품질향상의 이점이 있어 톳 정유를 이용한 명란젓의 제조방법을 본 발명에서 완성하였다.Accordingly, the inventors of the present invention is a natural antibacterial substance that is expected to inhibit the growth of the causative agent of spoiled spoiled salt during the study on how to improve the taste and quality of salted salted cod roe produced on the market More microbial, pH, amino nitrogen, VBN, and TBA measurements result in greater shelf life, reducing the amount of returns and wastes resulting from alteration, not only saving food resources, but also reducing product production costs There is an advantage of improving the production method of cod roe using 톳 essential oil was completed in the present invention.

상기 목적에 따라, 본 발명은 천연 항균성 물질로서 톳 정유를 이용하여 품질 향상 및 저장성이 우수한 명란젓을 제조하기 위한 제조방법을 제공한다.In accordance with the above object, the present invention provides a manufacturing method for producing cod roe excellent in quality improvement and shelf life by using essential oils as natural antibacterial substances.

구체적으로 본 발명은 명태로부터 명란을 채취하여 수세하는 선별 및 정선의 제 1단계; 상기 제 1단계의 명란에서 수분을 제거하고 절임물에 침지하여 1일 내지 4일, 바람직하게는 1일 내지 3일 동안 염장시키는 제 2단계; 상기 제 2단계의 염장된 명란에서 불순물 및 수분을 제거하는 제 3단계; 상기 제 3단계의 불순물이 제거된 명란에 조미하고 조미된 명란에 톳 정유를 첨가한 마른양념을 바르는 제 4단계; 상기 마른 양념이 가미된 명란에 물양념을 바르는 제 5단계를 포함함을 특징으로 하는 제조공정으로부터 본 발명의 톳 정유를 이용하여 품질향상 및 저장성이 우수한 톳 정유를 첨가한 명란젓을 수득할 수 있다. Specifically, the present invention comprises the first step of screening and selection to collect and wash water from pollack pollack; A second step of removing water from the first step and immersing in pickles to salt for 1 to 4 days, preferably 1 to 3 days; A third step of removing impurities and water from the salted egg of the second step; A fourth step of applying a dry seasoning to the seasoned cod roe and the seasoned cod roe added to the seasoned cod roe; Using the 톳 essential oil of the present invention from the manufacturing process characterized in that it comprises a fifth step of applying a water spice to the dried seasoned cod roe, can be obtained cod roe added with 톳 essential oil with excellent quality and shelf life. .

본 발명에서 사용되는 절임물은 명란 100 중량부 대비하여 소금 1 내지 20중량부, 설탕 1 내지 10중량부, 물 1 내지 10중량부, 및 청주 0.01 내지 5중량부, 바람직하게는 명란 75 내지 85 중량부에 소금 1 내지 15중량부, 설탕 1 내지 10중량부, 물 1 내지 10중량부, 및 청주 0.01 내지 5중량부로 조성되어 있는 것을 특징으로 한다.The pickles used in the present invention are 1 to 20 parts by weight of salt, 1 to 10 parts by weight of sugar, 1 to 10 parts by weight of water, and 0.01 to 5 parts by weight of sake, preferably 75 to 85, relative to 100 parts by weight of egg. It is characterized by comprising 1 to 15 parts by weight of salt, 1 to 10 parts by weight of sugar, 1 to 10 parts by weight of water, and 0.01 to 5 parts by weight of sake.

본 발명에서 사용되는 마른양념은 명란 100 중량부 대비하여 고춧가루 1 내지 10중량부, 설탕 0.01 내지 5중량부, 및 톳 정유 0.01 내지 10중량부, 바람직하게는 명란 75 내지 85 중량부에 고춧가루 1 내지 5중량부, 설탕 0.1 내지 3중량부 및 톳 정유 0.1 내지 6 중량부로 조성되어 있는 것을 특징으로 한다.Dried seasoning used in the present invention is 1 to 10 parts by weight of red pepper powder, 0.01 to 5 parts by weight of sugar, and 0.01 to 10 parts by weight of essential oil, preferably 75 to 85 parts by weight of red pepper powder, relative to 100 parts by weight of egg. 5 parts by weight, 0.1 to 3 parts by weight of sugar and 0.1 to 6 parts by weight of essential oils, characterized in that the composition.

본 발명에서 사용되는 물양념은 명란 100 중량부 대비하여 고춧가루 1 내지 7중량부, 미원 0.01 내지 5중량부, 마늘 1 내지 10중량부, 물엿 1 내지 10중량부, 물 1 내지 15중량부, 바람직하게는 명란 75 내지 85 중량부에 고춧가루 1 내지 5중량부, 미원 0.1 내지 3중량부, 마늘 2 내지 7중량부, 물엿 2 내지 7 중량부, 물 1내지 10중량부로 조성되어 있는 것을 특징으로 한다.Water seasoning used in the present invention is 1 to 7 parts by weight of red pepper powder, 0.01 to 5 parts by weight, 1 to 10 parts by weight of garlic, 1 to 10 parts by weight of starch syrup, 1 to 15 parts by weight of water, preferably 100 parts by weight of egg It is characterized in that it is composed of 1 to 5 parts by weight of red pepper powder, 0.1 to 3 parts by weight of miwon, 2 to 7 parts by weight of garlic, 2 to 7 parts by weight of starch syrup and 1 to 10 parts by weight of water. .

본원에서 정의되는 톳 정유는 국내산 또는 수입산 톳, 바람직하게는 국내산 톳, 보다 바람직하게는 톳류(Hizikia fusifirmia)을 건조 및 분쇄하여 절단하고, 상기 시료 총 중량의 약 1배 내지 200배(w/w), 바람직하게는 10배 내지 100배(w/w)의 정제수를 포함한 물, 주정, 탄소수 1 내지 4의 저급 알콜, 또는 이들의 혼합용매, 바람직하게는 물 또는 물 및 에탄올 혼합용매, 보다 바람직하게는 물 또는 50-99% 물 및 에탄올 혼합용매를 가하여 10분 내지 7일, 바람직하게는 20분 내지 2시간 동안, 0℃ 내지 150℃, 바람직하게는 10℃ 내지 100℃, 보다 바람직하게는 20℃ 내지 90℃에서 냉침추출, 열수추출, 초음파 추출, 환류냉각 추출 등의 추출방법, 바람직하게는, 초음파추출법을 수행하여 추출물을 수득하는 제 1단계; 상기에서 얻은 추출물을 여과하고 여과물을 감압 건조시키는 제 2단계의 제조공정을 통하여 본 발명의 톳으로부터 추출한 톳 정유을 수득할 수 있다.
톳 Essential oils as defined herein are cut by drying and grinding domestic or imported 톳, preferably domestic 톳, more preferably Hizikia fusifirmia, and from about 1 to 200 times the total weight of the sample (w / w). ), Preferably water containing 10 to 100 times (w / w) purified water, alcohol, lower alcohols having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably water or a mixed solvent of water and ethanol, more preferably Preferably, water or 50-99% water and ethanol mixed solvent are added for 10 minutes to 7 days, preferably 20 minutes to 2 hours, 0 ° C to 150 ° C, preferably 10 ° C to 100 ° C, more preferably Extraction method of cold sedimentation, hot water extraction, ultrasonic extraction, reflux cooling extraction, etc. at 20 ° C. to 90 ° C., preferably, a first step of obtaining an extract by performing an ultrasonic extraction method; The crude oil extracted from the oil of the present invention can be obtained through the second step of manufacturing the filtered extract and drying the filtrate under reduced pressure.

이하, 본 발명의 명란젓의 제조방법을 상세히 설명한다.Hereinafter, the manufacturing method of the cod roe of the present invention will be described in detail.

제 1단계에서 북태평양 또는 동해에서 어획된 명태로부터 명란을 채취하여 수세하는 선별 및 정선을 수행하는 단계; Performing a selection and selection process of picking and washing the flag from the roasted seafood in the North Pacific or the East Sea in the first step;

제 2단계에서 상기 제 1단계의 명란에서 수분을 제거하고 절임물에 침지하여 1일 내지 4일, 바람직하게는 1일 내지 3일 동안 침지시키는 단계; Removing water from the first step of the egg in the second step and immersing in pickles to immerse for 1 to 4 days, preferably 1 to 3 days;

제 3단계에서 상기 제 2단계의 공정으로 처리한 명란은 불순물 및 수분을 제거시키는 단계; In the third step, the egg that has been treated in the process of the second step may include removing impurities and moisture;

제 4단계에서 상기 제 3단계의 불순물이 제거된 명란에 조미하고 조미된 명란에 마른양념을 바르는 단계; Applying seasoning to the seasoned cod roe and dried seasoned cod roe in a fourth step;

제 5단계에서 상기 마른 양념이 가미된 명란에 물양념을 바르는 제 5단계를 포함하는 제조공정으로부터 본 발명의 톳 정유를 이용하여 품질 향상 및 저장성이 우수한 명란젓을 제조할 수 있다.From the manufacturing process comprising the fifth step of applying the water seasoning to the dried seasoned cod roe in the fifth step using the 톳 essential oil of the present invention can be produced excellent cod roe.

또한 본 발명은 상기 제조방법으로 수득되는 품질 향상 및 저장성이 우수한 톳 정유를 첨가한 명란젓을 제공한다.
In another aspect, the present invention provides a spicy cod roe added 톳 essential oils excellent in quality and storage properties obtained by the manufacturing method.

상기한 제조 공정으로 제조한 명란젓이 시중에서 판매되는 명란젓보다 미생물, 효모, 유산균, pH, VBN 및 TBA 측정결과, 품질 및 저장성이 탁월하여 최종적으로 변질로 인한 식량자원의 낭비를 막고, 제품의 생산 경비를 절감시킬 뿐만 아니라, 제품의 품질향상의 이점을 확인하였다.
Cod roe prepared by the above manufacturing process is excellent in microbial, yeast, lactic acid bacteria, pH, VBN and TBA measurement results, superior quality and shelf life than commercially available cod roe to prevent waste of food resources due to deterioration. In addition to reducing costs, we have confirmed the benefits of product quality improvement.

본 발명은 천연 항균성 물질인 톳 정유를 이용하여 본 발명의 공정으로 제조한 명란젓이 시중에서 판매되는 명란젓보다 미생물, 효모, 유산균, pH, VBN 및 TBA 측정결과, 품질 및 저장성이 탁월하여 최종적으로 변질로 인한 식량자원의 낭비를 막고, 제품의 생산 경비를 절감시킬 뿐만 아니라, 제품의 품질향상의 이점이 있기 때문에 식품학적 가치를 향상시키는데 기여할 수 있다.
The present invention is a microbial, yeast, lactic acid bacteria, pH, VBN and TBA measurement results, quality and shelf life superior to the cod roe produced by the process of the present invention using a natural antibacterial substance 톳 essential oil, finally deteriorated It not only prevents waste of food resources, reduces the production cost of the product, but also contributes to improving the food value because it has the advantage of improving the quality of the product.

이하, 본 발명을 실시예 및 실험예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

단, 하기 실시예 및 실험예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예 및 실험예에 의해 한정되는 것은 아니다.
However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples and experimental examples.

참고예Reference Example 1. 시료의 준비 1. Preparation of sample

명란은 속초에서 어획된 명태로부터 동결된 명란을 채취하여 사용하였고, 소금(천일염, 강릉시 수협), 설탕(백설탕, CJ), 청주(백화수복, 롯데주류), 미원(감칠맛나는 미원, 청정원), 물엿(옛날물엿, 오뚜기), 마늘(강릉시 하나로마트), 수돗물(강릉시), 고춧가루(해찬들고춧가루, CJ)을 구입 사용하였다.
The cod roe was harvested from frozen pollock caught in Sokcho and used as salt (sun salt, Gangneung-si Suhyup), sugar (white sugar, CJ), cheongju (baekhwa restoring, Lotte liquor), Miwon (flavorful beauty, clean water), Starch syrup (old starch syrup, Ottogi), garlic (Gangneung-si, Hanaro Mart), tap water (Gangneung-si), and red pepper powder (Haechan deul pepper, CJ) were used.

참고예Reference Example 2. 톳 정유 제조 2. oil refinery manufacturing

톳 정유로 사용한 톳(Hizikia fusifirmia)은 강릉시 수협에서 구입하여 사용하였으며, 분말화된 톳(200 g 건조중량)에 95% ethanol 1,000 mL를 첨가 후 30℃에서 30분 동안 초음파추출기(Power Mega21R)로 초음파 추출법(sonication)을 수행한 후에 감압 여과하여 에탄올 추출물을 얻어 이를 감압농축기(rotary evaporator; Heating Bath B-490)로 40℃에서 용매를 제거한 후 -40℃이하에서 보관하면서 실험에 사용되었다.
H Hizikia fusifirmia was used as a refined oil in Gangneung-si, and was added to 1,000 g of 95% ethanol in powdered sorghum (200 g dry weight) with an ultrasonic extractor (Power Mega21R) for 30 minutes at 30 ℃. After performing sonication, the resultant was filtered under reduced pressure to obtain an ethanol extract, which was then used in an experiment while removing the solvent at 40 ° C. with a rotary evaporator (Heating Bath B-490) and storing it at −40 ° C. or lower.

실시예Example 1. 명란젓 제조  1. Cod roe manufacture

1-1. 톳 정유1-1. Oil refinery To 첨가한 명란젓 제조 Added cod roe

상기 참고예 1의 냉동된 명란을 2일정도 상온에서 해동하여 세척하였다. 세척한 명란에 명란양(중량) 대비 소금 10%(중량), 설탕 4%(중량), 물 40%(중량), 청주 0.5%(중량)의 간수로 48시간동안 염장을 하였다. 절인 명란을 세척, 선별 후 채반에서 3시간 동안 물기를 빼고, 명란양(중량) 대비 고춧가루 3%(중량), 설탕 0.7%(중량), 농도를 달리한 상기 톳 정유 1%(중량) 또는 3%(중량) 첨가하여 마른 양념을 하였다. 최종적으로 물양념을 명란양(중량) 대비 고춧가루 2%(중량), 미원 0.15%(중량), 마늘 5%(중량), 물엿 4.5%(중량), 물 6.5%(중량)을 첨가하여 명란젓을 제조하여 멸균팩에 담아 25℃에 저장한 후에 하기 실험에 사용하였다.(이하, 톳 정유 1% 및 3%라고 각각 명명함).Frozen frozen egg of Reference Example 1 was washed by thawing at room temperature for about 2 days. The washed cod roe was salted for 48 hours with salt of 10% (weight), sugar 4% (weight), water 40% (weight), and 0.5% (weight) of sake. After washing and sorting the pickled cod roe, drain the water for 3 hours in the rice tray, and add 3% (weight) of pepper powder, 0.7% (weight) of sugar, 1% (weight) of the above-mentioned essential oil with different concentrations. % (Weight) was added to dry seasoning. Finally, add 2% (weight) of red pepper powder, 0.15% (weight) of garlic, 5% (weight) of garlic, 4.5% (weight) of starch syrup, and 6.5% (weight) of water. Prepared and stored in a sterile pack and stored at 25 ℃ was used in the following experiments (hereinafter referred to as 톳 essential oil 1% and 3%, respectively).

1-2. 1-2. 솔빈산을Solvin acid 첨가한 비교용 명란젓 제조 Preparation of Comparative Cod roe

상기 1-1 공정중 톳 정유 대신에 시중에서 식품보존제로서 사용되는 솔빈산(S1626-250G, SIGMA)을 각각 1% 및 3%(중량%)를 대체하여 첨가하는 것만 제외하고는 상기 1-1 명란젓 제조공정과 동일한 방법을 이용하여 솔빈산을 첨가한 비교용 명란젓을 제조하였다(이하, “솔빈산 1% 및 3%”라고 각각 명명함).
1-1 except for adding sorbinic acid (S1626-250G, SIGMA), which is used as a food preservative, in place of 1% and 3% (% by weight), respectively, in place of the essential oil in step 1-1. A comparative cod roe added with sorbic acid was prepared using the same method as the cod roe manufacturing process (hereinafter, referred to as “solvinic acid 1% and 3%”, respectively).

1-3. 1-3. 포도씨유를Grapeseed oil 첨가한 비교용 명란젓 제조 Preparation of Comparative Cod roe

상기 1-1 공정중 톳 정유 대신에 시중에서 식품보존제로서 사용되는 포도씨유(오뚜기)을 각각 1% 및 3%(중량%)를 대체하여 첨가하는 것만 제외하고는 상기 1-1 명란젓 제조공정과 동일한 방법을 이용하여 포도씨유을 첨가한 비교용 명란젓을 제조하였다(이하, 포도씨유 1% 및 3% 라고 각각 명명함).
In the process of 1-1, except for adding 1% and 3% (wt%) of grapeseed oil (OTTOGI), which is used as a food preservative in the market, instead of essential oil, A comparative cod roe with grapeseed oil was prepared using the same method (hereinafter, referred to as grapeseed oil 1% and 3%, respectively).

1-4. 1-4. 트레할로스를Trehalose 첨가한 비교용 명란젓 제조 Preparation of Comparative Cod roe

상기 1-1 공정중 톳 정유 대신에 시중에서 식품보존제로서 사용되는 트레할로스(Trehalose, , 제품번호PREHA, HAYASHYBARA) 을 각각 1% 및 3%(중량%)를 대체하여 첨가하는 것만 제외하고는 상기 1-1 명란젓 제조공정과 동일한 방법을 이용하여 트레할로스을 첨가한 비교용 명란젓을 제조하였다(이하, Trehalose 1% 및 3% 라고 각각 명명함).
Except for replacing 1% and 3% (wt%) of trehalose (Trehalose, product number PREHA, HAYASHYBARA), which is used as a food preservative in the market, instead of the essential oil in 1-1 above, A comparative cod roe with trehalose was prepared using the same method as the -1 cod roe manufacturing process (hereinafter, referred to as Trehalose 1% and 3%, respectively).

실험예Experimental Example 1. 톳 정유 첨가 명란젓의 미생물 수 및  1. The number of microorganisms of cod roe with essential oil pHpH 측정  Measure

1-1.미생물 수 측정1-1. Number of microorganisms

상기 실시예에서 제조된 명란젓에 대해 25(±5)℃에 3, 6, 9, 12일간 저장하면서 미생물 수를 하기와 같이 측정하였다. The number of microorganisms was measured as described below for 3, 6, 9, and 12 days storage at 25 (± 5) ° C for the cod roe prepared in Example.

저장 중 명란젓의 미생물 총균수는 3M Aerobic Count Plate film(55144-1000), 유산균수는 BCP첨가 한천배지(DIFCO(E-MB31))를 사용하였다. The total number of microorganisms in the broth was 3M Aerobic Count Plate film (55144-1000) and the number of lactic acid bacteria was BCP-supplemented agar medium (DIFCO (E-MB31)).

상기 실험 결과, 톳 정유를 활용한 고품질 명란젓의 숙성기간에 따른 총균수의 변화는 표 1와 같다. 톳 정유 첨가 명란젓의 초기 총균수는 일반 명란젓의 총균수와 비교하여 크게 차이를 보이를 않았으나, 저장 3일째부터 9일째까지 총균수가 급격히 증가한 일반 명란젓에 비해 톳 정유 첨가 명란젓은 저장기간 동안 초기 총균수와 비교하여 크게 차이를 보이지 않았다.As a result of the experiment, the total bacterial count according to the ripening period of high-quality cod roe using 젓 essential oil is shown in Table 1. 초기 The initial total bacterial count of essential cod roe was not significantly different from that of normal cod roe, but compared to the common cod roe whose total number of bacteria increased sharply from day 3 to 9 There was no significant difference compared to the number of bacteria.

저장중(25±0.5℃) 톳 정유 첨가 명란젓의 총균수 변화 (단위: CFU/g)During storage (25 ± 0.5 ℃) 변화 Total bacterial count changes of cod roe with essential oils (Unit: CFU / g) 시료sample 저장기간(일)Storage period (days) 00 33 66 99 ControlControl 7.1×105 7.1 x 10 5 1.1×107 1.1 × 10 7 1.9×109 1.9 × 10 9 2.2×109 2.2 x 10 9 솔빈산 1%Solvate 1% 6.6×105 6.6 × 10 5 6.5×105 6.5 × 10 5 5.7×105 5.7 × 10 5 7.4×108 7.4 × 10 8 솔빈산 3%Solvate 3% 6.4×105 6.4 × 10 5 4.6×105 4.6 × 10 5 4.7×105 4.7 × 10 5 5.3×108 5.3 × 10 8 포도씨유 1%1% grape seed oil 6.8×105 6.8 × 10 5 5.9×105 5.9 × 10 5 5.2×108 5.2 × 10 8 7.9×106 7.9 × 10 6 포도씨유 3%3% grape seed oil 8.5×105 8.5 × 10 5 3.8×106 3.8 × 10 6 9.6×108 9.6 × 10 8 1.6×109 1.6 × 10 9 Trehalose 1%Trehalose 1% 5.8×105 5.8 × 10 5 5.2×107 5.2 × 10 7 3.6×108 3.6 × 10 8 1.1×109 1.1 × 10 9 Trehalose 3%Trehalose 3% 4.8×105 4.8 × 10 5 1.2×106 1.2 × 10 6 4.2×106 4.2 × 10 6 1.2×107 1.2 × 10 7 톳 정유 1%톳 1% essential oil 4.6×105 4.6 × 10 5 4.7×105 4.7 × 10 5 4.3×105 4.3 × 10 5 4.9×105 4.9 × 10 5 톳 정유 3%톳 3% essential oil 5.7×105 5.7 × 10 5 3.1×105 3.1 × 10 5 9.4×105 9.4 × 10 5 3.6×105 3.6 × 10 5

유산균수Lactobacillus 측정 Measure

톳 정유를 활용한 고품질 명란젓의 숙성기간에 따른 유산균수의 변화는 표 2와 같다. 저장 6일째 일반 명란젓에 비해 톳 정유 첨가 명란젓의 유산균수가 높게 나타났으며, 저장 9일째는 약 10배 이상으로 높게 나타났다. 총균수는 일반 명란젓이 높았으나, 톳 정유 첨가 명란젓의 유산균수가 상대적으로 높게 나타난 결과를 바탕으로 톳 정유를 첨가가 명란젓중의 유산균수 상승이라는 발효제품 품질 지수를 향상시킴을 확인하였다.변화 Changes in the number of lactic acid bacteria according to ripening period of high-quality cod roe using essential oil are shown in Table 2. On the 6th day of storage, the number of lactic acid bacteria of the fermented cod roe with the essential oil was higher than that of the general cod roe, and on the ninth day of storage, it was about 10 times higher. The total number of bacteria was high in general cod roe, but based on the results of the relatively high lactic acid bacteria of cod roe, it was confirmed that addition of cod essence improved fermentation product quality index.

저장중(25±0.5℃) 톳 정유 첨가 명란젓의 유산균수 변화 (단위: CFU/g)During storage (25 ± 0.5 ℃) 변화 Change in the number of lactic acid bacteria of cod roe with essential oil (unit: CFU / g) 시료sample 저장기간(일)Storage period (days) 66 99 ControlControl 3.4×104 3.4 × 10 4 5.7×104 5.7 × 10 4 톳 정유 1%톳 1% essential oil 3.1×105 3.1 × 10 5 1.0×106 1.0 × 10 6 톳 정유 3%톳 3% essential oil 7.8×105 7.8 × 10 5 5.1×105 5.1 × 10 5

1-2. 1-2. pHpH 측정 Measure

상기 실시예에서 제조된 명란젓에 대해 25(±5)℃에 3, 6, 9, 12일간 저장하면서, pH 측정은 시료 10 g을 취해서 증류수 20 mL를 가해 균질화 시킨 후 pH meter(Mettler Toledo, SevenEasy pH, switzerland)로 측정하였다.
For 3, 6, 9, 12 days storage at 25 (± 5) ℃ for the cod roe prepared in the above Example, pH measurement was homogenized by taking 10 g of sample and adding 20 mL of distilled water and then pH meter (Mettler Toledo, SevenEasy pH, switzerland).

상기 실험 결과, 톳 정유를 활용한 고품질 명란젓의 숙성기간에 따른 pH 변화는 표 3과 같다. 초기 명란젓의 pH는 4.7-6.1 로 시료간에 유의적 차이를 보였다. 일반 명란젓은 저장 12일까지 지속적으로 pH가 증가한 반면에, 항산화제 및 항균제 첨가 명란젓과 톳 정유 첨가 명란젓은 pH 변화가 없거나 감소하였다.As a result of the experiment, the pH change according to the ripening period of high-quality cod roe using 한 essential oil is shown in Table 3. The pH of the early cod roe was 4.7-6.1, which showed a significant difference between samples. General cod roe increased up to 12 days of storage, whereas cod roe with antioxidants and antimicrobial agents and cod roe with ointment showed no or decreased pH.

저장중(25±0.5℃) 톳 정유 첨가 명란젓의 pH 변화During storage (25 ± 0.5 ℃) pH Change of pH of cod roe with essential oil 시료sample 저장기간(일)Storage period (days) 00 33 66 99 1212 ControlControl 6.0±0.0a 6.0 ± 0.0 a 6.0±0.0c 6.0 ± 0.0 c 6.0±0.0b 6.0 ± 0.0 b 6.3±0.1a 6.3 ± 0.1 a 6.1±0.0b 6.1 ± 0.0 b 솔빈산 1%Solvate 1% 5.2±0.0c 5.2 ± 0.0 c 5.1±0.0f 5.1 ± 0.0 f 5.0±0.0g 5.0 ± 0.0 g 5.0±0.0h 5.0 ± 0.0 h 5.0±0.0f 5.0 ± 0.0 f 솔빈산 3%Solvate 3% 4.7±0.0d 4.7 ± 0.0 d 4.9±0.0g 4.9 ± 0.0 g 4.9±0.0h 4.9 ± 0.0 h 4.8±0.0i 4.8 ± 0.0 i 4.7±0.0g 4.7 ± 0.0 g 포도씨유 1%1% grape seed oil 6.0±0.0a 6.0 ± 0.0 a 6.0±0.0d 6.0 ± 0.0 d 5.8±0.0de 5.8 ± 0.0 de 5.9±0.0d 5.9 ± 0.0 d 6.2±0.0a 6.2 ± 0.0 a 포도씨유 3%3% grape seed oil 6.0±0.0a 6.0 ± 0.0 a 6.0±0.0c 6.0 ± 0.0 c 6.1±0.0a 6.1 ± 0.0 a 6.2±0.0b 6.2 ± 0.0 b 6.2±0.0a 6.2 ± 0.0 a Trehalose 1%Trehalose 1% 6.0±0.0a 6.0 ± 0.0 a 5.9±0.0e 5.9 ± 0.0 e 5.9±0.0c 5.9 ± 0.0 c 6.0±0.0c 6.0 ± 0.0 c 6.2±0.0a 6.2 ± 0.0 a Trehalose 3%Trehalose 3% 6.1±0.0a 6.1 ± 0.0 a 5.9±0.0e 5.9 ± 0.0 e 5.8±0.0e 5.8 ± 0.0 e 5.6±0.0g 5.6 ± 0.0 g 5.5±0.0e 5.5 ± 0.0 e 톳 정유 1%톳 1% essential oil 6.0±0.1a 6.0 ± 0.1 a 6.1±0.0a 6.1 ± 0.0 a 5.8±0.0d 5.8 ± 0.0 d 5.8±0.0e 5.8 ± 0.0 e 5.8±0.0c 5.8 ± 0.0 c 톳 정유 3%톳 3% essential oil 6.0±0.0b 6.0 ± 0.0 b 6.0±0.0b 6.0 ± 0.0 b 5.7±0.0f 5.7 ± 0.0 f 5.7±0.0f 5.7 ± 0.0 f 5.8±0.0d 5.8 ± 0.0 d Values are mean±SD (n=3).
a- iValues with the same letter are not significantly different(p<0.05).
Values are mean ± SD (n = 3).
a- i Values with the same letter are not significantly different (p <0.05).

실험예Experimental Example 2. 신선도( 2. Freshness ( VBNVBN , 휘발성 염기 질소) 측정 , Volatile base nitrogen) measurement

상기 실시예 1에서 제조된 명란젓에 대해 25(±5)℃에 3, 6, 9, 12일간 저장하면서 하기와 같이 신선도를 측정하였다.Freshness was measured as described below while storing the cod roe prepared in Example 1 at 25 (占 5) 占 폚 for 3, 6, 9, and 12 days.

시료 2 g을 취해 증류수 16 mL를 가하고 20% TCA용액 2 mL를 넣고 잘 마쇄한 다음 10분간 방치한 후 여과하여 상징액을 취한 후 Conway unit를 이용한 미량 확산법으로 휘발성 염기질소의 양을 구하였다. Take 2 g of sample, add 16 mL of distilled water, add 2 mL of 20% TCA solution, grind well, leave for 10 minutes, filter, collect supernatant, and quantify volatile basic nitrogen by microdiffusion method using Conway unit.

톳 정유를 활용한 고품질 명란젓의 숙성기간에 따른 VBN 변화는 표 4와 같다. 초기 VBN함량은 19.3-23.6 mg% 이였으며 일반 명란젓에 비해 톳 정유 첨가 명란젓과 포도씨유, Trehalose 첨가 명란젓의 VBN함량이 높게 나타났다. 저장 3일째부터는 톳 정유 첨가 명란젓이 일반 명란젓뿐만 아니라 솔빈산, 포도씨유, Trehalose 첨가 명란젓보다 VBN함량이 낮게 나타났으며, 저장 마지막 날까지 47.7-220.4 mg%로 유의적인 차이를 보였다. 이 결과, 톳 정유 첨가로 인해 명란젓의 부패를 크게 억제한 것을 확인하였으며, 솔빈산, 포도씨유, Trehalose와 같은 항산화제와 항균제보다 효과가 좋음을 확인하였다.변화 VBN changes according to aging period of high quality cod roe using essential oil are shown in Table 4. The initial VBN content was 19.3-23.6 mg% and the VBN content of cod roe, grapeseed oil, and trehalose cod roe was higher than that of cod roe. From the 3rd day of storage, VBN content was lower than that of solvate, grapeseed oil and Trehalose as well as general cod roe. As a result, it was confirmed that the addition of 톳 essential oil significantly suppressed the corruption of spicy cod roe, and is more effective than antioxidants and antibacterial agents such as sorbic acid, grape seed oil, Trehalose.

저장중(25±0.5℃) 톳 정유 첨가 명란젓의 VBN , 변화 (단위: mg%)During storage (25 ± 0.5 ℃) V VBN , change of cod roe with essential oil (unit: mg%) 시료sample 저장기간(일)Storage period (days) 00 33 66 99 1212 ControlControl 19.8±1.3c 19.8 ± 1.3 c 44.8±2.1a 44.8 ± 2.1 a 115.9±18.7a 115.9 ± 18.7 a 156.9±5.2b 156.9 ± 5.2 b 197.9±2.0b 197.9 ± 2.0 b 솔빈산 1%Solvate 1% 19.3±0.0c 19.3 ± 0.0 c 44.9±0.5a 44.9 ± 0.5 a 50.6±0.9de 50.6 ± 0.9 de 52.1±1.4d 52.1 ± 1.4 d 66.6±1.6e 66.6 ± 1.6 e 솔빈산 3%Solvate 3% 19.6±1.1c 19.6 ± 1.1 c 42.1±1.6b 42.1 ± 1.6 b 43.5±1.6de 43.5 ± 1.6 de 50.5±1.2d 50.5 ± 1.2 d 68.6±0.6e 68.6 ± 0.6 e 포도씨유 1%1% grape seed oil 21.4±1.0bc 21.4 ± 1.0 bc 40.9±1.1b 40.9 ± 1.1 b 54.6±2.7d 54.6 ± 2.7 d 111.9±0.9c 111.9 ± 0.9 c 198.7±0.8b 198.7 ± 0.8 b 포도씨유 3%3% grape seed oil 21.8±0.7bc 21.8 ± 0.7 bc 29.2±1.0d 29.2 ± 1.0 d 66.0±4.6c 66.0 ± 4.6 c 155.6±1.8b 155.6 ± 1.8 b 188.4±8.0c 188.4 ± 8.0 c Trehalose 1%Trehalose 1% 22.8±2.2b 22.8 ± 2.2 b 32.0±0.3c 32.0 ± 0.3 c 88.6±0.6b 88.6 ± 0.6 b 177.0±4.7a 177.0 ± 4.7 a 220.4±4.1a 220.4 ± 4.1 a Trehalose 3%Trehalose 3% 23.1±0.3b 23.1 ± 0.3 b 31.8±0.7c 31.8 ± 0.7 c 86.5±1.6b 86.5 ± 1.6 b 113.9±3.0c 113.9 ± 3.0 c 169.4±5.4d 169.4 ± 5.4 d 톳 정유 1%톳 1% essential oil 22.5±1.1b 22.5 ± 1.1 b 28.2±0.7d 28.2 ± 0.7 d 40.2±2.1e 40.2 ± 2.1 e 45.2±1.1e 45.2 ± 1.1 e 47.7±0.7f 47.7 ± 0.7 f 톳 정유 3%톳 3% essential oil 26.0±2.1a 26.0 ± 2.1 a 28.1±1.1d 28.1 ± 1.1 d 38.7±2.6e 38.7 ± 2.6 e 49.2±1.2de 49.2 ± 1.2 de 51.9±1.4f 51.9 ± 1.4 f Values are mean±SD (n=3).
a- fValues with the same letter are not significantly different(p<0.05).
Values are mean ± SD (n = 3).
a- f Values with the same letter are not significantly different (p <0.05).

실험예 3. 항산화능 (Thiobarbituric acid(TBA); 지질산화) 측정 Experimental Example 3. Measurement of Thiobarbituric acid (TBA)

상기 실시예 1에서 제조된 명란젓에 대해 25(±5)℃에 3, 6, 9, 12일간 저장하면서 하기와 같이 항산화능을 측정하였다.The antioxidant activity of the cauliflower prepared in Example 1 was measured at 25 (占 5) 占 폚 for 3, 6, 9 and 12 days as follows.

마쇄한 명란젓 20g을 취하여 4℃로 냉각된 2M 인산이 포함된 20% 트리클로로아세트산(20% Trichloroacetic acid in 2M phosphoric acid)용액 50mL를 1.5분간 균질하게 마쇄시킨 후 마쇄액을 100mL 정용 플라스크로 정용하였다. 이 액 50mL를 와트만(WhaTBAn No. 1) 여과지로 여과한 후 원심분리기(미량원심분리기; Mega 21R) 15000ppm에서 10분간 돌린 후 상층액만 5mL 취해 실린지로 여과하여 시험관에 옮긴 후 0.005M 2-TBA(thiobarbituric acid) 5mL를 넣었다. 발색된 액을 530nm에서 흡광도(Jasco, JP/V-550)를 측정하여 측정된 흡광도에 5.2를 곱한 수치로 하였다20 g of the shredded cod roe was poured into 50 mL of 20% trichloroacetic acid in 2M phosphoric acid solution containing 2 M phosphoric acid cooled at 4 ° C for 1.5 minutes, and the resulting solution was used in a 100 mL constant volume flask . After filtrating 50 mL of this solution with a filter of WhaTBAn No. 1, the solution was turned for 10 minutes at 15000 ppm of a centrifuge (Micro Centrifuge; Mega 21R), and only 5 mL of the supernatant was filtered through a syringe, and then transferred to a test tube. 5 mL of thiobarbituric acid (TBA) was added thereto. The absorbed solution (Jasco, JP / V-550) was measured at 530 nm, and the absorbance measured was multiplied by 5.2.

톳 정유를 활용한 고품질 명란젓의 숙성기간에 따른 TBA 변화는 표 5과 같다. 저장 9일째 까지 톳 정유 첨가에 따른 명란젓의 항산화효과는 확인 할 수 없었으나, 저장 12일째 톳 정유 1%를 첨가하였을때 일반 명란젓보다 TBA값이 낮았다. 이 결과로, 톳 정유 1%를 첨가하였을때 명란젓의 산화를 억제 하는것을 확인하였다.변화 TBA changes according to the maturation period of high-quality cod roe with essential oil are shown in Table 5. Antioxidant effect of cod roe was not confirmed by the addition of essential oil until 9th day of storage. As a result, it was confirmed that the addition of 1% of essential oil inhibits the oxidation of cod roe.

저장중(25±0.5℃) 톳 정유 첨가 명란젓의 TBA변화 (단위: O.D)During storage (25 ± 0.5 ℃) 변화 Change in TBA of cod roe with essential oil (Unit: OD) 시료sample 저장기간(일)Storage period (days) 00 33 66 99 1212 ControlControl 0.1±0.0d 0.1 ± 0.0 d 0.1±0.0cde 0.1 ± 0.0 cde 0.1±0.0bc 0.1 ± 0.0 bc 0.1±0.0d 0.1 ± 0.0 d 0.6±0.2ab 0.6 ± 0.2 ab 솔빈산 1%Solvate 1% 0.1±0.0bc 0.1 ± 0.0 bc 0.2±0.0b 0.2 ± 0.0 b 0.3±0.0a 0.3 ± 0.0 a 0.4±0.0a 0.4 ± 0.0 a 0.5±0.0abc 0.5 ± 0.0 abc 솔빈산 3%Solvate 3% 0.1±0.0b 0.1 ± 0.0 b 0.1±0.0de 0.1 ± 0.0 de 0.3±0.0a 0.3 ± 0.0 a 0.4±0.0a 0.4 ± 0.0 a 0.5±0.0abc 0.5 ± 0.0 abc 포도씨유 1%1% grape seed oil 0.1±0.0d 0.1 ± 0.0 d 0.2±0.0a 0.2 ± 0.0 a 0.1±0.0bc 0.1 ± 0.0 bc 0.2±0.0b 0.2 ± 0.0 b 0.5±0.0abc 0.5 ± 0.0 abc 포도씨유 3%3% grape seed oil 0.1±0.0d 0.1 ± 0.0 d 0.2±0.0a 0.2 ± 0.0 a 0.2±0.0b 0.2 ± 0.0 b 0.2±0.0bc 0.2 ± 0.0 bc 0.4±0.0c 0.4 ± 0.0 c Trehalose 1%Trehalose 1% 0.1±0.0d 0.1 ± 0.0 d 0.1±0.0e 0.1 ± 0.0 e 0.1±0.0c 0.1 ± 0.0 c 0.2±0.0bc 0.2 ± 0.0 bc 0.5±0.0abc 0.5 ± 0.0 abc Trehalose 3%Trehalose 3% 0.1±0.0cd 0.1 ± 0.0 cd 0.1±0.0e 0.1 ± 0.0 e 0.1±0.0bc 0.1 ± 0.0 bc 0.2±0.0bcd 0.2 ± 0.0 bcd 0.6±0.0a 0.6 ± 0.0 a 톳 정유 1%톳 1% essential oil 0.1±0.0bcd 0.1 ± 0.0 bcd 0.1±0.0cd 0.1 ± 0.0 cd 0.1±0.0bc 0.1 ± 0.0 bc 0.2±0.0cd 0.2 ± 0.0 cd 0.4±0.0bc 0.4 ± 0.0 bc 톳 정유 3%톳 3% essential oil 0.2±0.0a 0.2 ± 0.0 a 0.1±0.0c 0.1 ± 0.0 c 0.1±0.0bc 0.1 ± 0.0 bc 0.2±0.0bc 0.2 ± 0.0 bc 0.4±0.0a 0.4 ± 0.0 a Values are mean±SD (n=3).
a- dValues with the same letter are not significantly different(p<0.05).
Values are mean ± SD (n = 3).
a- d Values with the same letter are not significantly different (p <0.05).

Claims (7)

명태로부터 명란을 채취하여 수세하는 선별 및 정선의 제 1단계; 상기 제 1단계의 명란에서 수분을 제거하고 절임물에 침지하여 1일 내지 4일 동안 염장시키는 제 2단계; 상기 제 2단계의 염장된 명란에서 불순물 및 수분을 제거하는 제 3단계; 상기 제 3단계의 불순물이 제거된 명란에 조미하고 조미된 명란에 톳 정유를 첨가한 마른양념을 바르는 제 4단계; 상기 마른 양념이 가미된 명란에 물양념을 바르는 제 5단계를 포함함을 특징으로 하는 톳 정유를 첨가한 명란젓의 제조방법.The first step of selection and selection of pickling and pickling from pollack; A second step of removing water from the first step and dipping in pickles to salt for 1 to 4 days; A third step of removing impurities and water from the salted egg of the second step; A fourth step of applying a dry seasoning to the seasoned cod roe and the seasoned cod roe added to the seasoned cod roe; A method of manufacturing cod roe cod with 톳 essential oil, characterized in that it comprises a fifth step of applying water seasoning to the dried seasoned cod roe. 제 1항에 있어서, 상기 절임물은 명란 100 중량부 대비하여 소금 1 내지 20 중량부, 설탕 1 내지 10 중량부, 물 1 내지 10 중량부, 및 청주 0.01 내지 5 중량부로 조성되어 있는 것을 특징으로 하는 제조방법.The method of claim 1, wherein the pickles are 1 to 20 parts by weight of salt, 1 to 10 parts by weight of sugar, 1 to 10 parts by weight of water, and 0.01 to 5 parts by weight of sake based on 100 parts by weight of egg. Manufacturing method. 제 1항에 있어서, 상기 마른양념은 명란 100 중량부 대비하여 고춧가루 1 내지 10중량부, 설탕 0.01 내지 5중량부, 및 톳 정유 0.01 내지 10중량부로 조성되어 있는 것을 특징으로 하는 제조방법.The method according to claim 1, wherein the dried seasoning is composed of 1 to 10 parts by weight of red pepper powder, 0.01 to 5 parts by weight of sugar, and 0.01 to 10 parts by weight of essential oil, relative to 100 parts by weight of egg. 제 1항에 있어서, 상기 물양념은 명란 100 중량부 대비하여 고춧가루 1 내지 7중량부, 미원 0.01 내지 5중량부, 마늘 1 내지 10중량부, 물엿 1 내지 10중량부, 물 1 내지 15중량부로 조성되어 있는 것을 특징으로 하는 제조방법.According to claim 1, wherein the water seasoning is 1 to 7 parts by weight of red pepper powder, 0.01 to 5 parts by weight, 1 to 10 parts by weight of garlic, 1 to 10 parts by weight of starch syrup, 1 to 15 parts by weight of water, based on 100 parts by weight of egg The manufacturing method characterized by the above-mentioned. 제 1항에 있어서, 상기 톳 정유는 국내산 또는 수입산 톳으로부터 추출된 톳 정유임을 특징으로 하는 제조방법.The method of claim 1, wherein the refined oil is a refined oil extracted from domestic or imported oil. 삭제delete 제 1항의 제조방법으로 수득되는 품질 향상 및 저장성이 우수한 톳 정유를 첨가한 명란젓.Cod roe with added essential oil having excellent quality and shelf life obtained by the method of claim 1.
KR1020120013438A 2012-02-09 2012-02-09 A method for preparing the salted Alaska pollack roe using by Hizikia fusifirmia oil KR101330451B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020120013438A KR101330451B1 (en) 2012-02-09 2012-02-09 A method for preparing the salted Alaska pollack roe using by Hizikia fusifirmia oil

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020120013438A KR101330451B1 (en) 2012-02-09 2012-02-09 A method for preparing the salted Alaska pollack roe using by Hizikia fusifirmia oil

Publications (2)

Publication Number Publication Date
KR20130092012A KR20130092012A (en) 2013-08-20
KR101330451B1 true KR101330451B1 (en) 2013-11-15

Family

ID=49216942

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020120013438A KR101330451B1 (en) 2012-02-09 2012-02-09 A method for preparing the salted Alaska pollack roe using by Hizikia fusifirmia oil

Country Status (1)

Country Link
KR (1) KR101330451B1 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020024130A (en) * 2002-01-26 2002-03-29 손영석 Salted fish with a substance that becomes a medicine
KR20030094199A (en) * 2003-11-24 2003-12-11 김득기 Process For Making Salt Fish With Marine algae Powder, Mugwort, Green tea And Chitosan Solution
KR100736466B1 (en) 2006-03-29 2007-07-06 대경에프.엔.비 주식회사 Method for manufacturing citron taste pollack roe
KR20100137929A (en) * 2009-06-23 2010-12-31 강릉원주대학교산학협력단 A method for preparing the salted alaska pollack roe additional using by alginic acid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020024130A (en) * 2002-01-26 2002-03-29 손영석 Salted fish with a substance that becomes a medicine
KR20030094199A (en) * 2003-11-24 2003-12-11 김득기 Process For Making Salt Fish With Marine algae Powder, Mugwort, Green tea And Chitosan Solution
KR100736466B1 (en) 2006-03-29 2007-07-06 대경에프.엔.비 주식회사 Method for manufacturing citron taste pollack roe
KR20100137929A (en) * 2009-06-23 2010-12-31 강릉원주대학교산학협력단 A method for preparing the salted alaska pollack roe additional using by alginic acid

Also Published As

Publication number Publication date
KR20130092012A (en) 2013-08-20

Similar Documents

Publication Publication Date Title
CN108522633B (en) Formula and application of preservative and antistaling agent composition containing ferulic acid butyl ester
CN107198147A (en) A kind of method of low temperature two-part fermentation aid bag
KR101142655B1 (en) A method for preparing the salted Alaska pollack roe additional using by Wasabia Japonica Matsum
KR101373377B1 (en) A method for preparing the salted Alaska pollack roe using by bamboo salt
CN108669472A (en) A kind of tender goose of perfume and its production technology
KR101330451B1 (en) A method for preparing the salted Alaska pollack roe using by Hizikia fusifirmia oil
KR101373384B1 (en) A method for preparing the salted Alaska pollack roe using by kelp oil
KR101317691B1 (en) A method for preparing the salted Alaska pollack roe using by sea mustard oil
KR101373521B1 (en) A method for preparing the salted changran jeot using by laver oil
KR101142633B1 (en) A method for preparing the salted Alaska pollack roe additional using by fucoidan
KR101373497B1 (en) A method for preparing the salted changran jeot using by sea mustard oil
KR101317690B1 (en) A method for preparing the salted Alaska pollack roe using by laver oil
KR101142690B1 (en) A method for preparing the salted Alaska pollack roe additional using by Artemisia capillaris
CN110916109A (en) Low-fat fermented sausage added with maltitol and preparation method thereof
KR101373355B1 (en) A method for preparing the salted changran jeot using by kelp oil
KR101373510B1 (en) A method for preparing the salted squid jeot using by laver oil
KR20160088658A (en) Seasoned Meat Products Containing Natural Preservatives and Manufacturing Method Thereof
KR101373385B1 (en) A method for preparing the salted squid jeot using by kelp oil
KR101373467B1 (en) A method for preparing the salted changran jeot using by deep sea water salt
KR101373450B1 (en) A method for preparing the salted changran jeot using by purified salt
KR101892167B1 (en) A preparation method of squid products with long term storage
KR101373446B1 (en) A method for preparing the salted squid using by sea mustard oil
KR101373431B1 (en) A method for preparing the salted Alaska pollack roe using by purified salt
KR20070044125A (en) Lotus(nelumbo nucifera) leaf extract added kimchi
CN106819077A (en) A kind of rattan mother-in-law tea extraction Compound Water product antistaling agent

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20161027

Year of fee payment: 4

LAPS Lapse due to unpaid annual fee