KR20130081131A - Composition and health liquor containing the extract of dandelion, siberian-ginseng, red ginseng, cherokee rose, mugwort and ginkgo leaf - Google Patents

Abstract

PURPOSE: Extracts of Taraxacum offininale, Acanthopanax senticosus, red ginseng, Rosa laevigata, Artemisia princeps, and Ginkgo biloba L. are provided to suppress 5 alpha-reductase which is related to alopecia and prostatic hypertrophy, and to develop a composition for preventing alopecia or prostatic hypertrophy. CONSTITUTION: A composition for preventing alopecia contains one or more selected from extracts of Taraxacum offininale, Acanthopanax senticosus, red ginseng, Rosa laevigata, Artemisia princeps, and Ginkgo biloba L. The extracts are prepared using an ethanol solution. Alopecia is suppressed by inhibiting testosterone 5 alpha-reductase. A composition for preventing prostatic hypertrophy contains one or more selected from extracts of Taraxacum offininale, Acanthopanax senticosus, red ginseng, Rosa laevigata, Artemisia princeps, and Ginkgo biloba L.

Description

Composition and Health Liquor Added with Dandelion Extract, Prickly Pear Extract, Red Ginseng Extract, Golden Primrose Extract, Leafy Leaf Extract and Ginkgo Leaf Extract {Composition and Health Liquor Containing the Extract of Dandelion, Siberian-ginseng, Red ginseng, Cherokee rose, Mugwort and Ginkgo leaf }

The present invention relates to dandelion extract, prickly pear extract, red ginseng extract, golden primrose extract, leaf extract or ginkgo biloba extract added composition and healthy liquor, specifically by inhibiting testosterone 5α-reductase (testosterone 5α-reductase) The present invention relates to compositions and healthy strains capable of preventing hair loss and enlarged prostate.

In modern society, as the occurrence of hair loss becomes more serious due to side effects due to various stresses, environmental pollution, drug abuse, perm, dyeing, use of hair dressings, and genetic factors, there is increasing interest in hair growth or hair growth. .

The causes of hair loss include poor blood circulation, excessive male hormone action, excessive sebum secretion, deterioration of scalp function by dandruff and other bacteria, genetic factors, aging stress, etc. The cause is unknown. Also, in recent years, the number of people who suffer from hair loss due to changes in diet, increase of stress due to social environment, etc. is increasing.

One of the causes of alopecia is directly related to the excessive release of male hormone, and is called androgenetic alopecia, commonly called male pattern baldness. In the case of androgenetic alopecia, healthy hair gradually becomes thinner, shorter, and weaker, leading to brittleness, which is called miniaturization. The hair follicles, which shrink, become thin and invisible, turn into short hairs and eventually develop hair loss. Accordingly, many studies have recently been reported for the prevention and treatment of hair loss through inhibition of male hormone activity.

In male alopecia, testosterone, one of the male hormones, is converted by testosterone 5a-reductase to dihydrotestosterone (DHT), an active male hormone. Testosterone binds to androgen receptors to delay protein synthesis in hair follicle cells, resulting in shorter hair follicle growth and atrophy of hair follicles. In addition, in the process of androgenetic alopecia, sebum is excessively produced, resulting in hair loss with inflammation.

On the other hand, one of the diseases caused by excessive dehydrotestosterone is benign prostatic hyperplasia. In the past, prostatic hyperplasia was defined as a condition in which the prostate was enlarged to block the passage of urine in the lower bladder, causing urethral obstruction, which reduced the flow of urine, and histologically defined as proliferation of prostate epilepsy or epithelial cells of the prostate. .

However, recently, the pathological physiology of the disease is too complicated to be explained by the above definitions or concepts, and urinary urination, night urination, and strong and sudden urinary urges to urinate more than eight times a day in men over 50 years old ) Urinary tract and urinary bladder storage symptoms such as urinary urgency and delayed urine (a phenomenon of urinating when urine is seen when urine), urinary discontinuation (phenomena of the flow of urine), urinating force The prostate hyperplasia is defined as the 'appeal of the lower urinary tract symptoms' collectively referred to as a symptom indicating a bladder discharge disorder.

The disease can be treated with medications, including androgen inhibitors. Testosterone 5alpha-reductase inhibitor, one of the androgen inhibitors, is used to treat prostatic hypertrophy, and commercially available finasteride and dutasteride are sold. These drugs act to reduce the size of the prostate by inhibiting androgen action in androgen-sensitive prostates. Recent studies have reported that long-term use of acute urinary tract and surgery reduces the frequency of prostate hyperplasia, and studies on the prophylactic effects of prostate cancer are being actively conducted.

However, it takes a long time to take effect after taking it, and there is a side effect that decreases sexual function, so it is recommended to take it only when the prostate specific antigen (PSA) level is high and the prostate size is large. Although causative treatment is possible by resection of prostate tissue enlarged by surgical treatment and drug treatment, most patients are restricted to the elderly, and there are problems such as side effects and recurrence after surgery, which increases the expectation for simple drug therapy. have.

Meanwhile, dandelion ( Taraxacum offininale ) is a perennial herb that grows in fields or on the roadside, and has been used extensively as a herbal medicine in the East and West because it contains a large amount of useful pharmacological ingredients. In oriental medicine, dried dandelion is called pogongyoung, and it is known to work to lower the heat, to urinate well, to remove inflammation, to strengthen the stomach, to detoxify and to clear blood, and tonic, fever, and diuresis. It has been used for health, expectancy, expectation, and detoxification.

Acanthopanax senticosus ) is a deciduous broad-leaved shrub belonging to the family Armenian ginseng, such as ginseng. Prickly gingiva tree looks like wild ginseng and is also called Siberian Ginseng in Russia, America and Europe. Prickly Pear Tree has been found to contain a large amount of acantoside and various useful components with excellent biological activity. Thorn oak tree has been recognized as one of the health-promoting plants for a long time and has been studied variously for its composition and efficacy. Through such studies, it has been found that the useful components of prickly oak bark act on the innate and specific immune systems of animals to enhance the antimicrobial and anti-tumor effects of the host. In particular, chisanoside, a type of secotriterpenoid glycoside present in fruits and leaves of prickly oak tree, has been reported to be effective in anticancer activity and kidney toxicity resistance. In addition, it is confirmed that prickly glycoside glycosides contained in prickly pear fruit have an effective effect on bone marrow hematopoiesis by promoting the synthesis of RNA. . Psoriasis containing these various useful ingredients has been used as a medicine for treating stroke, neuralgia, arthritis, hypertension, nervous breakdown and diabetes in folk medicine or traditional medicine.

Red ginseng refers to red ginseng that is dried and dried by ginseng, and is defined in Korean law as "ginseng starch ginseng, steamed ginseng, or other ginseng starch." Efficacy of various diseases has been reported through clinical trials and studies, such as being known for being excellent in the efficacy of homeostasis (恒常 性) that always maintains the constitution of health. Like ginseng and white ginseng, red ginseng contains glycosides, panacen, polyacetylene compounds, nitrogen-containing compounds, flavonoids, vitamin B group, trace elements, enzymes, antioxidants, organic acids and amino acids. It is contained. In addition, red ginseng is not simply manufactured for long-term storage of ginseng, and a variety of new bioactive ingredients are produced during the manufacturing process that are beneficial to our body. That is, in the manufacturing process of red ginseng, the chemical composition is changed due to the partial molecular structure change due to the thermal decomposition of ginsenoside, resulting in a beneficial special ingredient. Maltol, a special ingredient of red ginseng which is not contained in other ginseng such as fresh ginseng or white ginseng (Maltol) and ginsenosides (Ginsenosides Rh1, Rh2, Rs1, Rs2, Rg3, Rg5, etc.) contains eight kinds of special ingredients, among which Maltol (Maltol) has an anti-aging function, Ginsenosides (Ginsenosides) Rh1, Rh2, Rs1, Rs2, Rg3, Rg5, etc.) are known to have excellent activity such as anti-cancer, anti-diabetic, anti-ulcer, anti-inflammatory, anti-aging, vasodilation, and anti stress.

The stem of Rosa laevigata is vine-like, spiny and long. The leaves are 3 bilobal, small leaves have sack, oval, 2 ~ 4 cm long, 1 ~ 2 cm butterfly and pointed. In oriental medicine, the fruit is tonic, gangjeong, convergence, antibacterial, etc. It has chronic diarrhea, polyuria, oil well, 유 ureo, cold sweat, chronic enteritis, lobster, uterine bleeding, etc. Prescribe for treatment.

Artemisia is a perennial herbaceous plant belonging to the Asteraceae family and has a unique aroma and taste. The young leaves are used for wormwood rice cake as food, and the pharmacological components of the leaves are isocoumarin, coumarin, dieterlaclactone, flavonoid, phellandrene, cuprol ( cuprol) and cardinene. The lower lobe warms the blood vessels and meridians (經脈), so the uterine and lower abdomen are weak and cold due to uterine bleeding, bleeding during pregnancy, hemostasis, nosebleeds, and hemostasis. In addition, Hacho is weak and cold, cold and painful symptoms in the abdomen, menstrual disorders, menstrual pain, daeha (帶下), etc. It is effective in eczema and itching of the skin. Pharmacological actions include hemostatic action, fungal action, bronchial smooth muscle relaxation action, antitussive expectoration action, sleep action, uterus excitement action, hypersensitivity shock protection, anticancer action, antibacterial action.

Ginkgo Leaves biloba L. ) has been used as a medicine for asthma and bronchitis for about 5,000 years, and it is still being evaluated. Recently, it is widely used in Asia and Europe as an agent for treating peripheral circulatory disorders, cerebrovascular disorders and asthma. The ginkgo biloba extract was patented in Europe in 1972 and is widely used as a therapeutic agent for the cardiovascular system. The pharmacological action of the ginkgo biloba leaves is mainly known to be based on flavonoid compounds and terpene compounds. Terpene compounds include bilovalide, gingcholides A, B, C, D, M, and J. Gingcholides are platelets induced by antagonists such as Adenosine diphosphate (ADP), arachidonic acid, collagen, and thrombin. It does not inhibit aggregation but platelet bow

Interest has been very high since it has been reported as a potent antagonist that selectively inhibits only platelet aggregation induced by adults. Gingcolide is a competitive reaction with platelet activators and is useful for asthma, bronchitis, senile dementia, allergies, heart disease, shock symptoms, rheumatic diseases and other circulatory diseases.

Republic of Korea Patent No. 10-0451885 (hair loss prevention pomegranate extract and cosmetic composition for preventing hair loss comprising the pomegranate extract) has been described for pomegranate extract having 5 alpha-reductase inhibitory activity and hair loss prevention cosmetic composition comprising the same It has not been described that the composition to which the dandelion extract, prickly pear extract, red ginseng extract, golden primrose extract, leafy leaf extract or ginkgo biloba extract is effective in inhibiting testosterone 5 alpha-reductase. Korean Patent Laid-Open Publication No. 10-2007-0056207 (Testosterone 5-alpha-reductase inhibitor containing grape root extract) contains a testosterone 5-alpha-reductase inhibitor composition containing an extract of grape root as an active ingredient for hair loss and prostate. Although it has been described that it is effective in preventing hypertrophy, it has been described that the composition to which dandelion extract, prickly pear extract, red ginseng extract, golden primrose extract, leafy leaf extract or ginkgo leaf extract has the effect of inhibiting testosterone 5 alpha-reductase. none.

Therefore, the present invention confirms that the composition to which the dandelion extract, prickly pear extract, red ginseng extract, golden primrose extract, leafy leaf extract or ginkgo biloba extract inhibits testosterone 5alpha-reductase, and prevents hair loss or enlarged prostate. The purpose is to develop and provide.

In order to achieve the above object, the present invention provides a composition for preventing hair loss, characterized in that it comprises any one or more selected from dandelion extract, prickly pear extract, red ginseng extract, golden primrose extract, leaf extract and ginkgo leaf extract. In the present invention, a testosterone 5 alpha-reductase inhibitory test was performed on 162 natural products to select materials showing an inhibition rate of 30% or more. As a result of the test, it was confirmed that the dandelion extract, prickly pear extract, red ginseng extract, golden primrose extract, leafy leaf extract or ginkgo leaf extract showed more than 30% inhibition rate.

On the other hand, in the composition for preventing hair loss of the present invention, the composition preferably comprises dandelion extract, prickly pear extract and red ginseng extract. This is because the highest synergistic effect is achieved when the dandelion extract, prickly pear extract, and red ginseng extract are combined together among the six samples showing the inhibition rate of 30% or more.

On the other hand, in the composition for preventing hair loss of the present invention, the composition may be alcohol as an example. The six materials selected in the present invention are obtained as extract powders, and then added to alcohol to produce alcohol in the form of cocktails.

On the other hand, in the composition for preventing hair loss of the present invention, the extract is preferably used an aqueous solution of ethanol as the extraction solvent. This is because useful extraction is easy when an aqueous ethanol solution is used.

On the other hand, in the composition for preventing hair loss of the present invention, the prevention of hair loss is to inhibit hair loss by inhibiting the testosterone 5 alpha-reductase enzyme associated with hair loss.

On the other hand, the present invention provides a composition for preventing prostatic hyperplasia, characterized in that it comprises any one or more selected from dandelion extract, prickly pear extract, red ginseng extract, golden primrose extract, leaf leaf extract and ginkgo leaf extract.

On the other hand, in the composition for preventing the enlargement of the prostate gland of the present invention, the composition preferably comprises dandelion extract, prickly pear extract and red ginseng extract.

On the other hand, in the composition for preventing the enlargement of the prostate gland of the present invention, the composition may be an example.

On the other hand, in the composition for preventing the enlargement of the prostate gland of the present invention, the extract is preferably used an aqueous solution of ethanol as the extraction solvent.

On the other hand, in the composition for preventing prostatic hyperplasia of the present invention, the prevention of prostatic hyperplasia is to inhibit hair loss by inhibiting the testosterone 5 alpha-reductase enzyme associated with prostatic hyperplasia.

According to the present invention, it was confirmed that dandelion extract, prickly pear extract, red ginseng extract, golden primrose extract, leafy leaf extract or ginkgo biloba extract have an inhibitory effect of testosterone 5alpha-reductase related to hair loss or enlarged prostate. .

Based on the results, the present invention can prepare a composition for preventing hair loss or a composition for preventing prostatic hyperplasia.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[ Example  1: 162 sample extract preparation method]

200 g of ethanol, 70% ethanol or water was added to 10 g of a sample prepared by grinding finely 162 natural products, and extracted for 1 hour using an ultrasonic cleaner. The extracts were concentrated under reduced pressure with a vacuum rotary concentrator (Eyela N-1000, Tokyo, Japan) to obtain 162 extracts. Testosterone 5 alpha-reductase activity inhibition was intended to test the 162 extracts at a concentration of 66.67 μg / ml.

On the other hand, testosterone 5 alpha-reductase enzyme required for this experiment was prepared as follows. The livers of rats fasted for 24 hours were perfused with ice-cold Crabs-Ringer phosphate buffer (pH 7.2) to remove blood. A 1.4-fold ice-cold 0.01 M Tris hydrogen chloride buffer (Tris-HCl buffer, pH 7.2) was added to homogenize and centrifuged at 3000 rpm and 4 degreeC for 10 minutes. The supernatant separated by centrifugation was centrifuged again at 7,000 rpm at 4 ° C. for 10 minutes, and the supernatant obtained by centrifuging the separated supernatant once more at 10,000 rpm at 4 ° C. for 10 minutes was used as the enzyme solution. The enzyme solution was cryopreserved at -70 ° C and used in the experiment.

For the experiment, 50 μl of Testosterone (500 μg / ml), 50 μl of the test solution obtained by dissolving the sample obtained above, 160 μl of the testosterone 5 alpha-reductase enzyme solution prepared above were mixed, and NADPH (0.77 mg). / Ml) 80 μl, 400 μl 2 mM dithiothreitol were added to initiate the reaction, and then incubated at 37 ° C. for 30 minutes. The reaction was stopped by dissolving an internal standard (p-hydroxybenzoic acid n-hexyl ester, 0.01 mg / mL) in dichloromethane and adding 820 μl. Then, vortexing (vortexing) to mix the upper and lower well, centrifuged for 10 minutes at 3,000 rpm, and after removing the upper layer, the dichloromethane layer was blown with nitrogen. Add 500 μl of methanol and measure the amount of testosterone remaining by high performance liquid chromatography (HPLC) to calculate the testosterone 5 alpha-reductase activity inhibition rate as shown in Equation 1 below. '

On the other hand, as a positive control group of testosterone 5 alpha-reductase activity inhibitory experiment, Finasteride (Finasteride), which is currently used as a testosterone 5 alpha-reductase inhibitor, and the female hormone family ethynylestradiol were used.

On the other hand, the measurement conditions of high performance liquid chromatography (HPLC) were as follows. As a column, Thermo Hypersil Gold C18 (150 × 4.6 mm) was used, and as a mobile phase, methanol and water were mixed at a ratio of 65:35. The flow rate was 1.0 ml / min, the column temperature was 40 ° C., the injection amount was 20 μl, and the detection wavelength was measured at 254 nm. The measurement was performed using the internal standard method, and the testosterone 5 alpha-reductase inhibition rate was expressed as a% value by the peak-area ratio (testosterone / internal standard) (Equation 1).

[Equation 1]

% Inhibition = 100 x {area of test sample-area of the sample which has been reacted for 30 minutes / (area of the sample which has been stopped for 0 minutes-area of the sample which has been reacted for 30 minutes)}

As a result, dandelion, prickly pear, red ginseng, golden primrose, leaf lobe, ginkgo biloba leaf were selected as the material showing more than 30% inhibition rate (see Tables 1, 2 and 3). On the other hand, 26% of the positive control finasteride (Finasteride), 46% of the other positive control group Ethinylestradiol (Ethinylestradiol).

Sample name Extraction solvent Edible or not Inhibition rate
(%) Sample name Extraction solvent Edible or not Inhibition rate
(%) Gold candle ethanol possible 3.22 Youppy 70% Ethanol Of - Golden cherry 70% Ethanol possible 38.05 Ripe 70% Ethanol possible - Snow Cordyceps ethanol possible 24.14 Inosugi 70% Ethanol possible - Cordyceps ethanol impossible 6.42 Ginkgo leaves 70% Ethanol possible 35.99 Rhododendron 70% Ethanol Of 25.48 Self 70% Ethanol Of - Mural 70% Ethanol impossible 29.09 Pine needles 70% Ethanol Of - Baekbokryung ethanol possible 19.27 Pine tree trunk 70% Ethanol Of - Boiling 70% Ethanol possible 29.35 Peony 70% Ethanol possible - White migraine 70% Ethanol possible - Valerian 70% Ethanol possible - Law 70% Ethanol Of - tardy 70% Ethanol possible - Ghost ethanol possible 11.99 Creation 70% Ethanol possible - Ejection 70% Ethanol possible 28.55 Chunma 70% Ethanol possible - Casualties 70% Ethanol possible 18.46 Astronomical building 70% Ethanol possible - Raspberry 70% Ethanol Of 18.32 Taro leaves 70% Ethanol Of - Inner bird 70% Ethanol impossible 9.26 Taro stem 70% Ethanol possible 29.95 Flower pot 70% Ethanol possible 21.69 Passerby 70% Ethanol impossible - Pecker 70% Ethanol possible 16.66 enjoyment 70% Ethanol possible - Leaf 70% Ethanol Of 7.33 Gold 70% Ethanol possible - Yanggang 70% Ethanol possible 11.57 Black Schisandra 70% Ethanol Of - Mature meat 70% Ethanol 4.23 History ethanol Of - Alder ethanol - Thong ethanol impossible - Oval 70% Ethanol 9.72 Tamarind ethanol possible - The 70% Ethanol - buckwheat Water extraction possible - Yugunpi ethanol possible - Mushroom Water extraction possible - buckwheat ethanol possible - Sobaekpi 70% Ethanol Of - Neck and neck ethanol Of - Pine needles 70% Ethanol Of - White oyster 70% Ethanol impossible - Sookmyung 70% Ethanol possible 28.00 Centipede 70% Ethanol Of - Reminder 70% Ethanol Of - A black protein 70% Ethanol possible - Bitter mint 70% Ethanol possible - Bokbunja ethanol possible - Sophora ethanol possible 20.24 Seokchangpo ethanol possible - Bark 70% Ethanol Of - Cactus ethanol possible - Gongji mushroom ethanol possible -

Sample name Extraction solvent Edible or not Inhibition rate
(%) Sample name Extraction solvent Edible or not Inhibition rate
(%) green tea Water extraction possible - Arrow Water extraction possible 15.66 칡 Water extraction possible - Celestial Water extraction possible 6.00 hepatica Water extraction possible - Roe deer
mushroom Water extraction possible 2.31 Cactus Water extraction possible - Sophora Water extraction possible - Sewage Water extraction possible - Tofu Water extraction possible - Bitter mint Water extraction possible - purslane Water extraction possible 1.88 Yugunpi Water extraction possible - Living place Water extraction possible 11.17 Cordyceps Water extraction impossible - Fingering mushroom Water extraction possible 17.21 Gugija Water extraction possible - Pyeongbyeop Water extraction possible 7.05 cinnamon Water extraction possible - Earth tree Water extraction Of 11.13 A goose Water extraction possible - Bokbunja Water extraction possible - Safflower Water extraction possible 1.66 Honggyeongcheon Water extraction impossible - Doduk Water extraction possible - Hwanggi Water extraction possible - Tosa Water extraction possible - White oyster Water extraction impossible 4.15 motherwort Water extraction possible - Angelica Water extraction possible 13.14 Origin Water extraction possible - Three muscles Water extraction Of 2.47 Seokchangpo Water extraction possible - Three hundred seconds Water extraction possible - Shovel Root Water extraction Of - Solo Water extraction possible 4.29 Neck and neck Water extraction Of - Snow Cordyceps Water extraction possible - Difference Water extraction possible - Mushroom Water extraction possible - Gold candle Water extraction possible - Alder Water extraction Of - Bellflower Water extraction possible - Sookmyung Water extraction possible - Valerian Water extraction possible - Palm tree Water extraction Of -

Sample name Extraction solvent Edible or not Inhibition rate
(%) Sample name Extraction solvent Edible or not Inhibition rate
(%) Persimmon Water extraction Of - Love 70% Ethanol possible 32.11 Revenge Water extraction impossible 17.66 Indwelling 70% Ethanol possible 13.37 칡 70% Ethanol possible - Ginseng 70% Ethanol possible 22.84 A goose 70% Ethanol possible 35.44 Purple sweet potato 70% Ethanol possible - Gam-cho 70% Ethanol impossible - Bad 70% Ethanol possible 7.81 Approve 70% Ethanol possible - Earth tree trunk 70% Ethanol Of 22.18 mistletoe 70% Ethanol possible - Earth 70% Ethanol possible 10.30 cinnamon ethanol possible - dermis 70% Ethanol possible 28.86 Gosam 70% Ethanol impossible - Millennium 70% Ethanol Of 3.56 Wasabi ethanol possible - Astronomical building 70% Ethanol possible 1.49 Kwak Hyang 70% Ethanol Of - Bark tree 70% Ethanol possible - Gugija 70% Ethanol possible - Gardener 70% Ethanol possible 15.83 Copper ethanol impossible - Taxi 70% Ethanol impossible 0.47 A fugitive 70% Ethanol possible - Lake Toshi 70% Ethanol Of 10.15 Roe deer mushroom ethanol possible - Palm tree 70% Ethanol Of 12.89 Stink wood ethanol Of - Aspirations 70% Ethanol Of - Angelica ethanol possible - And seconds 70% Ethanol possible - Dang Vine ethanol impossible - Sewage ethanol possible 2.86 Doduk 70% Ethanol possible - Honggyeongcheon ethanol impossible 8.77 Bellflower ethanol possible - Safflower ethanol possible - Doin 70% Ethanol possible - Safflower Seed 70% Ethanol Of 25.45 Solo ethanol possible - Hwanggi ethanol possible 22.24 Tofu ethanol possible - Yellowish white 70% Ethanol impossible - rattan 70% Ethanol impossible - boxwood 70% Ethanol Of - dandelion 70% Ethanol possible 35.30 Red ginseng 70% Ethanol possible 37.33 rowan 70% Ethanol possible - Fingering mushroom ethanol possible -

[ Example  2: preparation of active sample extract and Health stock  Produce]

 2 g of alcohol was added to 100 g of each of dandelion, prickly pear, red ginseng, golden cherry, leafy leaf, and ginkgo biloba and refluxed for 3 hours at 100 ° C in a reaction tank (MS-CM709). Since the form of the composition targeted in the present invention is liquor, alcohol was used as the extraction solvent. Dandelion extract, prickly pear extract, red ginseng extract, golden primrose extract, leafy leaf extract, ginkgo leaf extract prepared by the above method were concentrated under reduced pressure using a reduced pressure rotary concentrator (Eyela N-1000, Tokyo, Japan). The extract was concentrated under reduced pressure and lyophilized to obtain a sample of dandelion extract 23 g, prickly pear extract 24 g, red ginseng extract 7.5 g, golden primrose extract 18 g, leaf extract 21 g, ginkgo leaf extract 13 g.

Meanwhile, 10% by weight of the obtained extract was added to the soju to prepare a cocktail of healthy liquor.

[ Example  3: Testosterone from six selected active samples 5 Alpha - Reductase  Activity Inhibition Synergy Effect Assessment]

To evaluate the synergistic efficacy of six selected active samples. First, obtain 50% inhibition rate (IC ₅₀ ) of dandelion extract, prickly pear extract, red ginseng extract, golden primrose extract, leafy leaf extract or ginkgo biloba extract. The concentrations were mixed in various proportions (3, 4, 5, 6) and used for testosterone 5 alpha-reductase activity inhibition test. The measuring method was the same as that of Example 1.

As a result, IC ₅₀ was as Table 4 below.

sample Concentration (ug / ml) Inhibition rate (%) IC ₅₀ (ug / ml) Ethinyl estradiol
(Ethinylestradiol) 666.7 64.91
163.94 333.3 56.39 66.67 46.35 Finasteride
(Finasteride) 66.67 70.99
36.09 33.33 51.23 6.67 26.60
Dandelion extract 666.7 64.59
224.80 333.3 51.73 66.67 37.35 33.33 27.05
Golden Primrose Extract 666.7 53.81
548,52
333.3 41.72 66.67 36.63 33.33 24.27
Leaf extract 666.7 61.85
255.36
333.3 54.32 66.67 31.11 33.33 25.47
Prickly Pear Extract 666.7 66.72
377.95
333.3 45.62 66.67 35.31 33.33 29.70
Ginkgo Leaf Extract 666.7 70.39
193.66
333.3 51.07 66.67 35.37 33.33 22.11
Red Ginseng Extract 666.7 60.86
444.25 333.3 43.68 66.67 37.36 33.33 25.78

On the other hand, EC30% was as shown in Table 5.

dandelion
(占 퐂 / ml) Golden cherry
(占 퐂 / ml) Love
(占 퐂 / ml) Go away
(占 퐂 / ml) Ginkgo leaf
(占 퐂 / ml) Red ginseng
(占 퐂 / ml) Inhibition rate
(%) 40.84 30 50.15 30 52.57 30 14.97 30 54.68 30 25.36 30

As confirmed in Table 5, the dandelion extract 40.84 ㎍ / ㎖, Golden Primrose extract 50.15 ㎍ / ㎖, leafy leaf extract ㎍ / ㎖, thorny goose extract 14.97 ㎍ / ㎖, ginkgo biloba extract 54.68 ㎍ / ㎖, red ginseng extract 25.36 ㎍ / ㎖ Has been shown to inhibit testosterone 5alpha-reductase by 30%.

Meanwhile, the compounding ratio was determined using the EC30% concentration of Table 5, and three, four, five, and six samples were mixed, respectively, and 50 µl of the total volume was reacted to calculate the synergistic growth rate (%). 6). Synergy efficacy increase rate was calculated using the following equation (2).

&Quot; (2) "

Synergistic potency increase rate (%) = {(inhibition rate% -30%) / 30%} × 100

N0 dandelion
extract
(占 퐂 / ml) Golden cherry
extract
(占 퐂 / ml) Love
extract
(占 퐂 / ml) Go away
extract
(占 퐂 / ml) Ginkgo leaf
extract
(占 퐂 / ml) Red ginseng
extract
(占 퐂 / ml) Inhibition rate
(%) Synergistic efficacy growth rate (%) One 1/3 1/3 1/3 33.22 10.73 2 1/3 1/3 1/3 38.43 28.1 3 1/3 1/3 1/3 38.49 28.3 4 1/3 1/3 28.90 - 5 1/3 1/3 1/3 34.63 15.43 6 1/3 1/3 36.40 21.33 7 1/3 1/3 1/3 24.65 - 8 1/3 1/3 20.54 - 9 1/3 1/3 1/3 41.72 39.07 10 1/3 1/3 34.54 15.13 11 1/4 1/4 1/4 1/4 9.08 - 12 1/4 1/4 1/4 1/4 15.18 - 13 1/4 1/4 1/4 1/4 24.16 - 14 1/4 1/4 1/4 1/4 25.00 - 15 1/4 1/4 1/4 1/4 4.46 - 16 1/4 1/4 1/4 1/4 16.17 - 17 1/4 1/4 1/4 1/4 10.60 - 18 1/4 1/4 1/4 1/4 10.74 - 19 1/4 1/4 1/4 1/4 - - 20 1/5 1/5 1/5 1/5 1/5 8.97 - 21 1/5 1/5 1/5 1/5 1/5 - - 22 1/5 1/5 1/5 1/5 1/5 8.36 - 23 1/6 1/6 1/6 1/6 1/6 1/6 - -

As a result of the experiment, as shown in Table 6, there was no synergy effect in the composition of all six species, the highest at 39.07% in the composition of dandelion extract, prickly pear extract, red ginseng extract (NO. 9) mixed three species Synergy efficacy growth rate is shown.

On the other hand, NO. Synthesis ratio (%) of 9 (dandelion extract, prickly pear extract, red ginseng extract) was measured as shown in Table 7 below (Table 7). 50 μl of the total volume was added and reacted, and the synergy increase rate (%) was calculated by the above Equation 2.

NO dandelion
extract
(占 퐂 / ml) Go away
extract
(占 퐂 / ml) Red ginseng
extract
(占 퐂 / ml) Inhibition rate
(%) Synergy
% Growth One 1/3 1/6 1/3 + 1/6 44.12 47.05 2 1/3 1/3 + 1/6 1/6 18.68 - 3 1/3 1/9 1/3 + 1/5 22.43 - 4 1/3 1/3 + 1/5 1/9 34.49 14.96 5 1/6 1/3 1/3 + 1/6 18.40 - 6 1/3 + 1/6 1/3 1/6 24.13 - 7 1/9 1/3 1/3 + 1/5 12.70 - 8 1/3 + 1/5 1/3 1/9 18.23 - 9 1/3 + 1/6 1/6 1/3 34.11 13.70 10 1/6 1/3 + 1/6 1/3 33.25 10.83 11 1/3 + 1/5 1/9 1/3 26.06 - 12 1/9 1/3 + 1/5 1/3 16.54 -

Experimental results, as confirmed in Table 7, the composition of the dandelion extract, prickly pear extract, red ginseng extract showed the highest synergy efficacy growth rate of 47.05%.

Claims (10)

Hair loss prevention composition, characterized in that it comprises any one or more selected from dandelion extract, prickly pear extract, red ginseng extract, golden primrose extract, leaf extract and ginkgo leaf extract.
The method of claim 1,
The composition may comprise,
Hair loss prevention composition comprising dandelion extract, prickly pear extract and red ginseng extract.
The method of claim 1,
The composition may comprise,
Hair loss prevention composition characterized in that the liquor.
The method of claim 1,
The above-
A composition for preventing hair loss, characterized in that an aqueous solution of ethanol is used as the extraction solvent.
The method of claim 1,
The hair loss prevention,
A composition for preventing hair loss, characterized by inhibiting hair loss by inhibiting testosterone 5 alpha-reductase enzyme related to hair loss.
A composition for preventing prostatic hyperplasia, characterized in that it comprises any one or more selected from dandelion extract, thorny opi extract, red ginseng extract, golden primrose extract, leaf extract and ginkgo leaf extract.
The method according to claim 6,
The composition may comprise,
A composition for preventing prostatic hyperplasia, comprising dandelion extract, prickly pear extract and red ginseng extract.
The method according to claim 6,
The composition may comprise,
Prostate hypertrophy composition, characterized in that the liquor.
The method according to claim 6,
The above-
A composition for preventing prostate hyperplasia, characterized in that an aqueous solution of ethanol is used as the extraction solvent.
The method according to claim 6,
The prostate enlargement prevention,
A composition for preventing prostatic hyperplasia, characterized by inhibiting testosterone 5alpha-reductase enzyme associated with prostatic hypertrophy to inhibit prostatic hyperplasia.