KR20130035062A

KR20130035062A - Ginsenoside preparation method using yeast

Info

Publication number: KR20130035062A
Application number: KR1020110099310A
Authority: KR
Inventors: 이원서; 이중수; 박승옥; 최겸
Original assignee: 농업회사법인 강성바이오 주식회사
Priority date: 2011-09-29
Filing date: 2011-09-29
Publication date: 2013-04-08

Abstract

The present invention relates to a ginsenoside production method using yeast. Specifically, the present invention relates to a method for preparing ginsenoside by adding yeast to red ginseng extract. In addition, the present invention relates to a method for preparing ginsenoside by adding yeast to red ginseng extract and cheonnyeoncho extract. Thus, the present invention enables mass production of ginsenosides.

Description

Ginsenoside preparation method using yeast {Ginsenoside preparation method using yeast}

The present invention relates to a ginsenoside production method using yeast.

Saponins are a chemical compound called glycosides.

Plant roots, stems, leaves, bark, seeds, etc., previously known as non-nutritive substances, but recently discovered the anti-cancer, antioxidant, cholesterol lowering effect, began to emerge as a bioactive substance.

Saponin is the main pharmacological agent among various active ingredients of ginseng.

Ginseng saponins have a unique chemical structure that is different from those found in other plants, and their pharmacological effects are also unique, which means 'Ginseng Glycoside', which is called 'Ginsenoside'.

These ginsenosides exist in a total of 34 forms, but ginsenosides such as Ra, Rb1, Rb2, Rc, and Re are mainly present in ginseng.

In particular, red ginseng, which has been steamed and dried, has an increased content of Rg3, Rh1, Rh2, and compound K (compound K, 20-ObD-glucopyranosyl-20 (S) -protopanaxadiol), which are rarely present in ginseng. do. Rg3, Rh1, Rh2, Compound K (compound K, 20-O-b-D-glucopyranosyl-20 (S) -protopanaxadiol) is known as the most excellent anti-cancer and anti-cancer metastasis effect, it is easy to absorb and digest when ingested.

However, ginseng before being obtained as red ginseng has a difficulty in cultivation, a long growing period, and the manufacturing process of ginseng to red ginseng is complicated, making it difficult to manufacture the ginsenoside materials in large quantities.

Meanwhile, Cheonnyeoncho (Opuntia humifusa) is a native Korean cactus native to Chungnam. Opuntia ficus-indica var saboten, an exotic species and commonly known as palm cactus, grows to 1-2 m in size and has many long and thick spines, while the cheonnyeoncho has fluffy spines and grows to about 30 cm in size. Baeknyeoncho, which is grown on Jeju Island, is native to the tropics and is difficult to cultivate in the open field, while Korean native cactus, Chunnyeoncho, survives in the open field even in the dormant winter season, and is resistant to pests.

In order to solve the problems of the prior art as described above, the present invention is to provide a method for producing a large amount of ginsenosides present in red ginseng.

In addition, to increase the sugar component than the conventional red ginseng, and to provide a manufacturing method for increasing the amount of fatty acids, minerals, and dietary fiber additionally.

Ginsenoside production method according to a feature of the present invention for solving the above problems is characterized in that the culture of ginsenosides using red ginseng extract and yeast.

Ginsenoside manufacturing method according to another feature of the present invention is characterized by culturing ginsenosides using the red ginseng extract and cheonnyeoncho extract and yeast.

Ginsenoside manufacturing method of the present invention is an invention related to increasing the amount of ginsenoid existing in the existing red ginseng in a larger amount. In addition, the invention relates to increasing the content of sugar than conventional red ginseng.

Accordingly, the present inventors have made diligent research efforts to overcome the problems of the prior art, and when ginsenosides are prepared by the ginsenoside manufacturing method according to the present invention, the content of ginsenosides is greatly increased compared to the conventional red ginseng. It can be confirmed that it can be prepared by greatly increasing the content of sugar and completed the present invention.

Specifically, the yeast is added to the medium in which the red ginseng extract and the cheonnyeoncho extract are mixed using the yeast, and the content of ginsenosides is increased when the yeast is cultured. At this time, the yeast is preferably Saccharomyces cerevisiae KCTC 7920 strain, the content of the red ginseng extract and cheonnyeoncho extract is characterized in that the content of 0.8 ~ 1.5: 1 ~ 20.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Example

Example One

Take 30-35 g of red ginseng purchased from Geumsan, grind it, add 1L of 70% ethanol, and reflux 3-5 times for 3-5 hours. The extracted solution is cooled to room temperature, filtered, and the solvent is removed using a vacuum concentrator. And autoclaved red ginseng suspension was prepared. The red ginseng suspension thus prepared was inoculated with 1-5% of the total culture solution of yeast bacteria pre-cultured in Potato dextrose broth (PDB), added 1-5% molasses, and then slowly stirred at 25-50 ° C. While incubating for 5-6 days to prepare a red ginseng fermentation.

Example 2

Red ginseng fermented product was prepared in the same manner as in Example 1, except that red ginseng suspension was prepared by mixing red ginseng powder and Cheonnyeoncho powder in a ratio of 1:20 in the preparation of red ginseng suspension of Example 1.

Comparative example

Comparative example One

Red ginseng fermented product was prepared in the same manner as in Example 1, except that Bacillus subtilis bacteria were used instead of yeast.

Comparative example 2

Red ginseng fermented product was prepared in the same manner as in Example 2, except that Bacillus subtilis bacteria were used instead of yeast.

Comparative example 3

In the preparation of the red ginseng suspension of Comparative Example 1 and the preparation of the red ginseng fermented product, the suspension of the cheonnyeoncho and cheonnyeoncho fermentation were prepared by the same method except that the cheonnyeoncho powder was used instead of the red ginseng powder.

Comparative example 4

In the preparation of the red ginseng suspension of Example 1 and the preparation of the red ginseng fermentation product, the cheonnyeoncho suspension and the cheonnyeoncho fermentation were prepared by the same method except that the cheonnyeoncho powder was used instead of the red ginseng powder.

Experimental Example

Experimental Example One

In the case where red ginseng fermentation product and cheonnyeoncho fermentation product were prepared according to Example 1, Example 2 and Comparative Example 4, an experiment was conducted in which each ginsenoside content was investigated by HPLC method.

Specifically, 1 g of each concentrate of Example 1, Example 2, and Comparative Example 4 was weighed, and 50 ml of 70% ethanol was added thereto, extracted twice in an 80 ° C shaking incubator, and Whatman # 1 paper (Whatman # 1 paper) Filtered). The filtrate was again dried under reduced pressure using a vacuum concentrator and dissolved by adding 50 ml of distilled water. 2 ml of diethyl ether was added to 1 ml of the dissolved solution, followed by centrifugation at 1,500 rpm for 10 minutes to remove diethyl ether. Then, 1.5 ml of butanol was added thereto, mixed, and the butanol layer was recovered. After mixing the recovery process was repeated three more times. 1.5 ml of distilled water was added to the recovered butanol layer, mixed and centrifuged to remove the water layer. Thus, the process of removing the water layer after mixing distilled water was repeated two more times to wash the water-soluble impurities. The butanol layer thus obtained was dried by injecting N 2 gas at 40 ° C., and 0.5 ml of methanol was added thereto, and then filtered through a 0.45 μm membrane filter to use as a sample for HPLC analysis.

HPLC analysis was an Agilent 1100 series (Agilent 1100 series, Agilent Technologies Inc., CA, USA), and the column was used (Symmetry, Waters, USA). The mobile phase was acetonitrile (HPLC grade, Sigma, USA) and distilled water for HPLC, and the mobile phase was the proportion of solvent B using 10% (v / v) CH3CN (A) and 90% (v / v) CH3CN (B). 11% (11 minutes) to 16% (15 minutes), 22% (40 minutes), 33% (41 minutes), 42% (46 minutes), 44% (51 minutes), 48% (65 minutes), 11% (69 minutes) was changed sequentially and finally adjusted to 11% (85 minutes). The column temperature was set to 40 ° C., the flow rate to 1 ml per minute, and the UV wavelength to 203 nm. The analysis results of the ginsenoside content according to Example 1, Example 2 and Comparative Example 4 are shown in Table 1.

Gin Senocide Comparative Example 4 Example 1 Example 2 Re 13.8 16.86 79.35 Rg1 - 10.61 57.66 Rh2 - - 40.33 Compound K - 0.14 0.38

As shown in Table 1, the content of Re, Rg1, Rh2, and Compound K was confirmed to be significantly increased in Example 1 than in Comparative Example 4, and also in Example 2 than in the case of Example 1 It was confirmed that the increase. As a result, according to Example 1 and Example 2 it was confirmed that a large amount of ginsenosides can be prepared.

Experimental Example 2

The red ginseng decomposition product and the cheonnyeoncho degradation products according to Example 1, Example 2, and Comparative Examples 1 to 4 were hydrolyzed, and an experiment was conducted to compare the results.

Table 2 below shows the results.

division Total sugar content (mg / ml) Uronic acid (μg / μl) Comparative Example 1 26.8 ± 4.2 300 ± 9.4 Comparative Example 2 44.8 ± 6.1 378.7 ± 5.8 Comparative Example 3 41.2 ± 2.8 236.5 ± 13.4 Comparative Example 4 45.2 ± 1.8 251 ± 53.2 Example 1 37.2 ± 2.3 341 ± 13.6 Example 2 59.3 ± 2.5 442.3 ± 35.21

As can be seen in Table 2, Example 1 shows that the total sugar content and the uronic acid content are partially reduced compared to Comparative Examples 1 to 4, but in the case of Example 2, the total sugar content and the uronic acid content It was confirmed that compared with Comparative Examples 1 to 4 significantly increased.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. It is natural.

Claims

Ginsenoside production method comprising culturing ginsenosides using red ginseng extract and yeast.

Ginsenoside production method comprising culturing ginsenosides using red ginseng extract and cheonnyeoncho extract and yeast.