KR20080081544A - Novel method of producing ginsenosides by biotransformation of ginseng through liquidl culture of phellinus linteus strain - Google Patents

Abstract

A method for producing ginsenosides is provided to mass-produce rare ginsenosides of Rd, Rg2 and Rh1 through liquid-culturing of Phellinus linteus mycelium within a short time. To produce a bio-converted ginsenoside, a Phellinus sp. strain such as Phellinus linteus KCTC 0912BP is liquid-cultured at a temperature of 20-35 deg.C for 1-10 day(s) with agitation, wherein the bio-converted ginsenoside includes Rd, Rg2 or Rh1 and consists of mannose. Further, the bio-converted ginsenoside is fresh ginseng, dried ginseng or tiny-sized ginseng.

Description

Novel method of producing ginsenosides by biotransformation of ginseng through liquidl culture of Phellinus linteus strain}

1 is a liquid chromatogram showing the ginsenoside pattern of fresh ginseng according to the present invention.

Figure 2 is a liquid chromatogram showing the ginsenoside pattern produced by the bioconversion method of the present invention.

Figure 3 is a gas chromatogram showing the pattern of the constituent sugar of the red ginseng polysaccharide according to the present invention.

Figure 4 is a gas chromatogram showing the pattern of the constituent sugar of the situation mushroom fermented red ginseng polysaccharide produced by the bioconversion method of the present invention.

Figure 5 is a diagram showing the immune enhancing effect of the present situation mushroom fermented red ginseng.

The present invention relates to a novel method for producing ginsenosides from ginseng through liquid culture of Felius linteus mycelium using a bioconversion method, and more particularly, a situation mushroom mycelium among ginseng components containing a large amount of a substance having excellent pharmacological activity. The present invention relates to a method for producing a large amount of useful saponin components in a short time using the bioconversion method through the liquid culture method.

Known as 'the mysterious elixir of the East', Korean ginseng is recognized as the world's best quality thanks to natural conditions suitable for the growth of ginseng and long-term accumulated cultivation and processing technology, and it is recognized as a representative image product representing Korea. In particular, Korean ginseng contains a large amount of physiologically active substances such as saponins and polyphenols that other plants do not have at all, and it is known to be an excellent herbal medicine to date because of its excellent pharmacological effect.

Recent scientific research shows that ginseng contains various bioactive substances such as ginsenosides (ginseng saponins), polyacetylenes, polyphenolic compounds, organic acids, amino acids, peptides, polysaccharides, vitamins, etc. Has been found to have the best physiological activity, and the pharmacological action has been reported to suppress the central nervous system, promote protein synthesis, regulate immune function, insulin-like action, detoxification, anti-cancer and anti-cancer activity.

Saponins are glycosides composed of glycosides and aglycones and are widely distributed in the plant family.Ginseng saponins are characterized by the fact that the non-sugar portion is a dammarane-based triterpene. (protopanaxdiol) It is classified as a protopanaxtriol family and to date, 38 ginsenoside chemical structures have been identified.

Of these, five major ginsenosides (ginsenosides Rb1, Rb2, Rc, Re, and Rg1) account for over 80% of all ginsenosides. Ginsenosides contain glucose, rhamnose, It is known that pharmacological action is different depending on the type, number and location of the sugars to which the sugars such as arabinose and xylose bind. Among these ginsenoside derivatives, in particular, there have been many studies on the physiological activity of minor ginsenosides present in ginseng in small amounts. These minor ginsenosides, Rd, Rg3, Rh1 and Ck, are major It has been disclosed to have a better physiological activity than ginsenoside (Republic of Korea Patent Publication 10-2006-0062083).

Therefore, in recent years, attempts have been actively made to convert major ginsenosides to trace ginsenosides, including methods of heat treatment (Korean Patent Publication No. 1996-017670), methods of treating acids and alkalis (Korea Patent Registration 10 -0620107), a method of enzymatic treatment (Korean Patent Publication 10-02304002) and the like are known.

However, the method of treating heat or acid and alkali may decompose trace present ginsenosides and polysaccharides having other excellent activities, and as a result, other biological activities of ginseng may be eliminated. On the other hand, the enzyme treatment method requires a lot of enzyme compared to the amount of substrate, and there is an inefficient problem in reaction time and yield.

In the case of mushrooms, various studies have been recognized as a material with high physiological activity. Pelinus linteus, a type of situational mushroom, has an anticancer activity of 96.7%, and the polysaccharide of the pelinus linteus mycelium has been reported to effectively inhibit Sarcoma 180 ascites and solid cancer. The conversion of ginsenosides by higher fungi can be divided into solid culture method and liquid culture method according to the culture method of higher fungi. When cultivating higher fungi with ginseng as a substrate, the incubation time is too long and the production efficiency decreases. Productivity is emerging as a problem.

In particular, in the case of the solid culture of higher fungi, it can act as a problem of industrialization process as a production space problem, manpower consumption type process. Regarding such a solid culture, Republic of Korea Patent Publication 10-2006-0062083, Republic of Korea Patent Registration 10-0496666, Republic of Korea Patent Publication 10-2004-0108476 has been known. According to the above patents, ginseng itself including higher fungi is known to produce ginseng products through fermentation for about 14 days to 90 days on a solid medium.

In view of the above, the present invention has not been reported to date Ginsenoside Rd, which is present or not present in trace ginseng, dried ginseng, and red ginseng using a biotransformation method through liquid culture of a mycelial mushroom of Felinus linteus. The aim is to provide a method for mass production of Rg2 and Rh1 in a short time.

The above object of the present invention is to culture the S. mushroom Felinus linteus KCTC 0912BP strain and to prepare a red ginseng powder medium to inoculate the seed, then incubate in bulk and obtain a situation-fermented ginseng dry powder to reflux in butanol solution and then residue Was achieved by drying, adding methanol, dissolving, filtration and performing HPLC analysis.

A feature of the present invention is a production method capable of mass production of rare ginsenosides Rd, Rg2, and Rh1, which are present in trace ginseng, dried ginseng, and red ginseng, in a short period of time by bioconversion through mycelium liquid culture of Felinus linteus strains. It is completed by providing.

Hereinafter, the configuration of the present invention will be described in detail.

The present invention utilizes the Phellinus linteus strain ( Phellinus linteus, KCTC 0912BP) , which has excellent bioconversion activity of ginsenosides in ginseng components, and is novel and mass-produced rare ginsenovirus which is bioconverted through liquid culture to this strain. Provides a side production method. More specifically, the present invention is excellent in the bioconversion activity of ginsenosides Plinus linteus ( Phellinus) linteus , KCTC 0912BP) and a method for producing a polysaccharide comprising mannose composed of constituents of ginsenosides Rd, Rg2 and Rh1 obtained by liquid culture of the mycelium of the strain as well as the specific polysaccharide derived from the situation mushroom mycelium. to provide.

The present invention comprises the steps of culturing the mycelium of the Pellinus linteus strain in a pure medium of Potato dextrose agar (PDA) solid medium; Liquid culturing the mycelium cultured in PDA solid medium in a potato dextrose broth liquid medium, respectively; After washing 500 g of fresh ginseng with running water, put it in a 5L closed container and steam it for 5 hours using heated steam.The steamed product was dried for 1 hour at 50 ℃ of a hot air dryer, and then dried in a drying room to be 15% or less of moisture. Drying to prepare red ginseng to prepare a bioconversion material; Putting ginseng ginsenoside into a liquid medium containing the red ginseng powder obtained in the above step into a bioreactor together with the mycelia of the Felinus linteus strain and sterilizing ginseng; Analyzing the rare ginsenosides Rd, Rg2 and Rh1 and constituent sugars from the bioconverted situation mushroom fermented ginseng; The present invention is composed of the immune effect and toxicity test step of the situation fermented ginseng.

Another object of the present invention relates to a method for providing total saponin, wherein the ginsenosides obtained in a short time from the bioconversion process using the situation mushroom mycelium liquid culture method include Rd, Rg2 and Rh1.

Another feature of the present invention is the Felinus linteus strain ( Phellinus linteus The coexistence of Rd, Rg2 and Rh1 in ginsenosides bioconverted by KCTC 0912BP) by liquid culture has not been disclosed at all.

Hereinafter, the specific configuration of the present invention will be described in detail by way of examples, but the scope of the present invention is not limited only to these embodiments, and those skilled in the art will easily detour by attempting to make some design changes or changes in numerical values and processes. Although it can be carried out such inventions will belong to the scope of the present invention.

Example  One Felinus Linteus  Strain ( Phellinus linteus KCTC  0912 BP Mycelium spawn culture

Felinus linteus KCTC 0912BP strains used in the present invention were collected and collected in Seorak-san, Gangwon-do, and the strains deposited as patent strains at the Korea Research Institute of Biotechnology and Biotechnology Center (KCTC). The strain was found to have the best bioconversion activity as a result of our study. Cultivation conditions of mycelium seedlings were maintained at 25 ° C. in potato dextrose agar (PDA) medium for 7-10 days, and then the mycelium pieces were cut to approximately 0.5 cm in a cleanbench and then 100 mL potatoes. Into a 500 mL Erlenmeyer flask containing a dextrose broth complex medium was incubated for 10 days. The mycelium of the strain cultured sufficiently for 10 days was pulverized for 1 minute at 4,000 rpm with a grinder and inoculated with 10% (v / v) as a seed in a 1 L Erlenmeyer flask containing 200 mL to 300 mL of WIM sterilization medium (Table 1). After 10 days liquid culture and the culture was used as 10% (v / v) spawn. The liquid culture mycelium used as a seed was placed in a sterile blender and homogenized, and then inoculated into a container containing 5 L of WIM medium in a 7 L volume fermentor (Marubishi Co. Ltd.). The culture environment of the mycelium of Felinus linteus KCTC 0912BP strain was supplied while adjusting the air injection rate from 0.1vvm to 1vvm. At the air injection speed of 1vvm or more, mycelium tends to grow on the wall of the incubator due to the formation of a large amount of bubbles, so the air injection speed is adjusted to 1vvm or less. The culture temperature was installed in a culture chamber to adjust the room temperature to 20 ℃ to 35 ℃ incubated for 5 days to 10 days. The bioreactor was sterilized for 20 minutes at 121 ℃ using a sterilizer.

WIM badge composition Badge Ingredient Medium composition (g / L) Glucose 20 peptone 5 Yeast extract 6 KH ₂ PO ₄ One K ₂ HPO ₄ 0.5 MgSO ₄ 7H ₂ O 2 pH 5.5

Example 2 Preparation of Red Ginseng Powder

4 kg of fresh ginseng from which the brains were removed was washed with running water, and then placed in a 20 L closed container and steamed for 5 hours with heated steam. The steamed ginseng was put in a hot air dryer, dried at 50 ° C. for 1 hour, and then transferred to a drying chamber to naturally dry 15% or less of water. In order to use red ginseng as a bioconversion material, a red ginseng powder was prepared in a size of about 20 to 30 mesh using a separator.

Example 3 Mass Production of Ginseng Ginsenosides Using a Bioconversion System Through Liquid Culture of Mycelium of the Felinus Linteus Strains of the Present Invention

36L of WIM medium containing the red ginseng powder prepared in Example 2 was prepared, transferred to a 70L bioreactor (in situ fermenter), and sterilization was performed at 121 ° C. for 40 minutes. After dropping the temperature of the bioreactor to about 28 ° C. using a low temperature circulation tank, 4L of the strains of the mycelium of Felinus linteus KCTC0912BP strain cultured in Example 1 were inoculated and cultured in a 70L bioreactor using a conventional aseptic inoculation method. As a comparative example, when using raw ginseng powder or dry ginseng powder, 36L of WIM medium containing fresh ginseng powder or dry ginseng powder instead of red ginseng powder was prepared and transferred to a 70L bioreactor (in situ fermenter), followed by 40 minutes at 121 ° C. A sterilization process was performed. After dropping the temperature of the bioreactor to about 28 ° C. using a low temperature circulation tank, 4L of the strains of the mycelium of Felinus linteus KCTC0912BP strain cultured in Example 1 were inoculated and cultured in a 70L bioreactor using a conventional aseptic inoculation method. Fermented ginseng reaction conditions were supplied while adjusting the air injection rate from 0.1vvm to 1vvm. At the air injection speed of 1vvm or more, mycelium tends to grow on the wall of the incubator due to the formation of a large amount of bubbles, so the air injection speed is adjusted to 1vvm or less. The cultivation temperature was installed in a culture chamber to adjust the room temperature to 20 ℃ to 35 ℃ incubated for 1 to 10 days. After the end of the culture, the fermented ginseng was filtered with filter paper and dried in a drier at 8O < 0 > C for 8 hours to determine the weight of the situation fermented ginseng to obtain about 600 g / L of dry weight. The culture filtrate containing the extracellular polysaccharide and the ginseng fermentation extract component was concentrated to 60 Brix, mixed with the dried fermented ginseng and then dried at 60 ℃ for 5 hours in a dryer. The final dried product was powdered to a size of about 100 mesh using a separator to obtain a final 1.6 kg of situation fermented ginseng powder.

Example 4 Extraction of Ginsenosides of Bioconverted Ginseng

As a result of Example 3, in order to analyze the pattern of ginsenoside of the situation culture fermented ginseng cultured in large quantities, it was carried out in accordance with the example standards and test methods of the health functional food method ginseng products. In other words, 5 g of the sample that passed through 100 mesh was weighed and placed in a 250 mL reflux flask, 50 mL of water-saturated butanol solution was added, and refluxed and cooled in a water bath at 70 ° C. to 80 ° C. for 1 hour, cooled, and filtered. Transfer to aliquot. The above operation was repeated two more times for the residue. 50 mL of distilled water is added to the separating funnel and shaken vigorously until the water layer and the butanol layer are completely separated. The water layer (lower layer) was removed, the butanol layer was transferred to a concentrated flask with a constant weight, concentrated under reduced pressure in a water bath, 50 mL of ether was added to the residue, and the mixture was cooled to reflux for 30 minutes in a water bath at about 46 ° C, and ether was removed. The residue was dried to a constant weight to obtain an analytical dry sample according to the present invention.

Experimental Example 1

Bio-converted Strains of Ginseng Ginsenoside HPLC  analysis

Methanol was added to the dried fermented ginseng (sample) of Example 4, completely dissolved, filtered through a 0.45 μm Minisart RC-15 (Satorius) filter, injected into HPLC according to the following analysis conditions, and analyzed by ginseng ginsenoside analysis. The area of was compared. The column used Platinum C18 (100 A, 1.5 μl, 33 mm × 7 mm), and the water phase acetonitrile ratio was 80: 20 for the mobile phase, 75: 25 for the first 10 minutes, and 50: 15 minutes afterwards. The density gradient of water and acetonitrile was adjusted at the ratio of 50. The flow rate of the mobile phase was 1.2 ml / min and the chromatogram was obtained at 203 nm (Fig. 1, Fig. 2). Raw ginseng ginseng In the present invention, the characteristic ginsenoside change of the situation-fermented ginseng powder showed a significant increase of 2.5 to 10 times in Rd, Rg2 and Rh1 compared to the bioconversion using dry powder (Table 2). ).

division  Ginsenosides (mg / g total saponin) Saponin Type Rg1 Re Rf Rb1 Rc Rb2 Rg2 Rh1 Rd Fresh ginseng 10.48 41.12 22.33 3.01 12.52 12.51 6.76 0.94 3.21 Situation Fermented Ginseng 3.61 10.43 5.04 0.64 3.96 4.52 17.33 10.97 17.78

Experimental Example 2

Analysis of Polysaccharide of Bio-Converted Sacral Fermented Ginseng of the Invention

In order to extract polysaccharides from the situation fermented ginseng obtained according to Example 4

Situation Fermented ginseng dry powder (sample) 80 g of water 20 times was added to 4 g of fresh ginseng powder and red ginseng powder and refluxed for 3 hours. The extract was filtered and 95% ethanol was added in 4 times the amount of the filtrate, then left at 4 ° C. for 12 hours, followed by centrifugation. After centrifugation, the supernatant was removed and the precipitate was dried to obtain a polymer polysaccharide. The intrinsic polysaccharide derived from the contextual mycelium in the polysaccharide was measured according to the following experimental method as the content of Methyl α-D-mannopyranoside contained in the polysaccharide.

50 mg of the sample was precisely weighed and placed in a 20 mL glass container, and 10 mL of methanolic 0.75 N hydrochloric acid solution was charged with nitrogen, and then reacted in a water bath at 80 ° C. for 24 hours. The reaction solution was cooled to room temperature and then transferred to a 100 mL flask for distillation under reduced pressure, and concentrated under reduced pressure at 60 ° C. 20 mL of methanol was added to the dried product under reduced pressure, completely dissolved, filtered through a 0.45 μm filter, and then, 10 mL of the filtrate was concentrated under reduced pressure. 5mL of internal standard solution was added to the residue, followed by adding 1 mL of trimethylchlorosilane 0.5mL Hexamethyldisilazane and vigorously shaking for 3 minutes, followed by filtration to obtain a sample solution. After preparing 34.5 mg of the standard solution with the special reagent Methyl α-D-Mannopyranoside, precisely cool down, dissolve it by adding the internal standard solution, and make up to 50 mL. Then, 5 mL of this solution was added, 0.5 mL of TMCS and 1 mL of HMDS were prepared, and vigorously shaken for 3 minutes. The internal standard solution was prepared by precisely weighing 50 mg of Rhamnose, adding anhydrous pyridine to make exactly 100 mL.

The polysaccharide content was calculated by the area of the sample solution and the standard solution obtained by testing according to the following GC analysis method with the sample solution and standard solution prepared as described above.

The analyzer was GC (Varian 3800) and the analysis conditions were as follows.

Column: RTX-5 Capillary column (0.53mm ｘ 30m)

Detector: Hydrogen flame ionization detector (290 ℃)

Column Temp: After holding for 1 minute at 180 ℃, raise 4 ℃ by minute and keep at 220 ℃.

Injector Temp: 270 ℃

Carrier gas: N2 80psi, H2 40psi, Air 60psi

Injection volume: 1.0µl

The calculation method is as follows.

Methyl α-D-Mannopyranoside (%) = (Asi X AT X Ws) / (ATi X As X WT) X 100

As: Methyl α-D-Mannopyranoside peak area of standard solution

Asi: Peak area of internal standard in standard solution

AT: Methyl α-D-Mannopyranoside peak area of the sample solution

ATi: Peak area of internal standard material

Ws: standard sample (mg)

WT: Sample collection amount (mg)

As a result, while 14g of mannose per 1g of situationally fermented red ginseng powder, mannose was not analyzed at all in ginseng dried ginseng or red ginseng (FIG. 3, 4). Therefore, the situation-fermented ginseng powder according to the present invention contains polysaccharides derived from situation mycelium, and it was found that the types and contents of constituent sugars are completely different from the polysaccharides obtained from fresh ginseng, dried ginseng, and red ginseng.

Experimental Example 3

Of the present invention Investigation of Immune Enhancing Effect of Bio-Converted Situation Fermented Red Ginseng

<Culture and administration of test substance>

The present invention investigated the immune-enhancing effect on macrophages using saponins extracted from the fermented red ginseng powder prepared in Example 3. In order to measure the activity of macrophages, the amount of nitric oxide (NO) produced was determined. Nitrogen monoxide is a radical of low molecular weight and is known for its role in neurotransmitter function, blood coagulation and blood pressure regulation, and immune function against cancer cells. Macrophage-based Raw 264.7 cell line was obtained from the Korea Cell Line Research Foundation. DMEM containing 10% FBS was incubated in a cell culture medium at 37 ° C and 5% CO2 incubator and passaged twice a week. Raw 264.7 cell lines were mixed into 96 well plates at 5 × 105 cells / mL with DMEM containing 10% FBS, and adapted for 24 hours. The test substance was dissolved and treated using DMEM medium without FBS. After culturing for 24 hours, the amount of free NO was measured as a culture of each well. To quantify NO, 100ul of the supernatant was taken, the same volume of Griess reagent (Sigma; G4410) was mixed and reacted for 15 minutes, and the absorbance was measured at 540 nm with an ELISA reader. Sodium nitrite was used to prepare a standard calibration curve. NO concentrations of test substance treatment group and negative control group were analyzed by Student t-test. Significance was recognized when P <0.01.

<Immune Boosting Effect Measurement>

This study was conducted to evaluate the effect of enhancing macrophage immune function by treating nitric oxide (NO) production by treating four test substances such as situationally fermented red ginseng in mouse-derived macrophages (Raw 264.7). NO, synthesized by activated macrophages, is known to play an important role in activating the immune system and exerting antiviral and anticancer activity. As a result of this experiment, the concentration of NO was increased in all the test substances compared to the negative control group, especially in the case of the situation fermented red ginseng treatment group, 14.6 umol / L of NO production was much higher than in other test substances (Fig. 5). Therefore, in the case of the present invention fermented red ginseng, it was determined that the immune function control effect of macrophages is excellent. That is, it was confirmed that there was statistically significant (p <0.01) in all test substances compared to the negative control.

Experimental Example 4

Present invention bioconverted Fermented red ginseng  Toxicity Test

<Dietary diet and test substance administration>

Single dose toxicity test was performed in ICR series mice using the situation fermented red ginseng powder prepared in Example 3. After oral administration of fermented red ginseng products to mice, mortality, clinical signs, body weight, feed and water intakes, and GOT / GPT tests were evaluated for 14 days. Experimental animals were used after adapting a total of 40 weeks-old ICR mice produced in Daehan Biolink Co., Ltd. in the animal breeding room (22 ± 2 ℃) for one week. Five mice were selected for each group, and the litter used during the test period was sterilized at 121 ° C. for 15 minutes using a high-pressure steam sterilizer, and water was freely supplied to experimental animals in clean bottles.

The preparation of the test substance was divided into fermented red ginseng products into hot water extract and powder that passed through 100 mesh, and carefully mixed so as not to foam in the physiological saline by the dosage. Dosage was as shown in the table below. The test substance was orally administered using sonde, and the dose was calculated according to the body weight immediately before administration.

<Analysis Sample Collection and Sample Analysis>

All experimental animals were observed once a day at regular time for changes in general condition, toxic symptoms and death. Body weight measurements, feed and water intake were measured once every two days, and body weight was measured before administration of test substance and on the day before necropsy. For blood biochemical test, blood was left in the refrigerator for 2 hours and centrifuged to separate serum and GOT / GPT detection kit (Asan Pharmaceutical) was used.

As a result, no animals died in all groups and no clinical symptoms were observed. As a result of weight measurement, feed and water intake, no significant change was observed in both the male and female groups compared with the control group (Table 3). In addition, there were no abnormal symptoms observed in the blood biochemical test compared to the control group (Table 4), and autopsy showed no specific abnormalities of the organ organs.

Weight change rate gender Dose (mg / kg) 0 2 4 6 8 10 12 14 Elapsed date cock 0 23.7 ± 0.9 30.3 ± .1.1 32.3 ± 1.6 33.7 ± 1.5 34.4 ± 1.9 36.9 ± 2.0 37.6 ± 2.6 38.7 ± 2.3 Situation Fermented Red Ginseng Hydrothermal Extract 2000 24.9 ± 1.2 30.2 ± 0.9 31.5 ± 1.2 33.6 ± 1.8 34.5 ± 2.3 36.7 ± 1.9 36.9 ± 2.2 38.6 ± 2.3 Situation Fermented Red Ginseng Powder 2000 23.7 ± 0.9 30.3 ± 1.1 32.3 ± 1.2 34.4 ± 1.8 35.1 ± 1.8 37.5 ± 1.2 37.9 ± 2.0 39.4 ± 1.9 female 0 22.3 ± 0.9 26.2 ± 0.9 27.6 ± 0.9 27.9 ± 0.8 28.6 ± 0.8 29.4 ± 1.0 29.5 ± 0.7 30.7 ± 1.2 Situation Fermented Red Ginseng Hydrothermal Extract 2000 21.8 ± 1.3 25.6 ± 1.1 27.6 ± 1.8 28.4 ± 2.0 28.7 ± 2.5 30.5 ± 2.6 30.0 ± 2.8 31.4 ± 3.2 Situation Fermented Red Ginseng Powder 2000 21.2 ± 0.6 24.5 ± 1.9 27.1 ± 1.3 28.1 ± 0.7 28.3 ± 1.3 28.6 ± 1.0 29.7 ± 1.5 30.5 ± 2.8

Serum blood biochemical test cock Dose (mg / kg) 0 Situation Fermented Red Ginseng Extract Situation Fermented Red Ginseng Powder 2000 GOP (IU / L) 34.1 ± 0.7 31.3 ± 6.1 32.8 ± 2.9 GOP (IU / L) 10.2 ± 1.0 1.0.5 ± 1.0 9.6 ± 0.8 female Dose (mg / kg) 0 Situation Fermented Red Ginseng Extract Situation Fermented Red Ginseng GOP (IU / L) 20.6 ± 1 23.6 ± 1.8 26.9 ± 4.1 GOP (IU / L) 3.8 ± 0.5 4.6 ± 0.6 5.2 ± 1.6

* Units are expressed as mean ± standard deviation (IU / L).

As described in the above Examples and Experimental Examples, the present invention was carried out in the fresh ginseng, dried ginseng and red ginseng components by the powerful bioconversion enzyme of this strain through the liquid culture of the mycelia of the strain of Felinus linteus KCTC 0912BP. There is an effect of mass production of total saponin, including trace ginsenosides Rd, Rg2 and Rh1, which are traces or non-existent. In addition, the present invention is a Phellinus strain ( Phellinus) linteus KCTC 0912BP) bioconverted using liquid culture method is a very useful invention in the biopharmaceutical industry because it has an excellent effect including the rare ginsenoside as well as mannose which is a specific polysaccharide component derived from the strain.

Claims (6)

Method for producing a bioconversion ginsenoside produced from the liquid culture of the genus Felinus. The method according to claim 1, wherein the strain of the genus Felinus is Phellinus linteus KCTC 0912BP. The bioconverted ginseng powder according to claim 1 or 2, wherein the ginsenoside bioconverted by the situation mushroom mycelium fermentation comprises Rd, Rg2 or Rh1 and the polysaccharide component consists of mannose. The bioconverted ginseng powder extract according to claim 1 or 2, wherein the ginsenoside bioconverted by the situation mushroom mycelium fermentation comprises Rd, Rg2 or Rh1 and the polysaccharide component consists of mannose. Phellinus according to claim 1 or 2 linteus KCTC 0912BP) sterilized with red ginseng powder in the liquid culture medium of the strain, the air injection speed is 0.1vvm to 1vvm, the stirring speed is 50 ~ 500 rpm, the culture temperature is 20 ℃ to 35 ℃ for 1 to 10 days Situation of culture mushroom culture method. 6. The method according to claim 5, wherein the raw material for bioconversion ginseng comprises fresh ginseng, dried ginseng or rice ginseng.

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