KR20130033041A - Quantitative estimating water-proofing quality of cosmetics goods by turbidity analysis - Google Patents

Abstract

PURPOSE: A quantitative method with respect to water resistance of a makeup product through turbidity analysis is provided to objectively and visually evaluate water resistance through the turbidity level, and to enhance reliability of the makeup product. CONSTITUTION: A quantitative method with respect to water resistance of a makeup product through turbidity analysis comprises: a step of preparing a film with a groove, and applying the makeup product on the film before drying; a step of treating the film by ultrasonic waves for a predetermined time; a step of putting the film into a tube containing a buffer solution, and heating to separate the makeup product and the film; a step of centrifuging the solution in the tube; and a step of collecting a supernatant and measuring turbidity. [Reference numerals] (AA) Test group; (BB) Control group; (CC) Before ultrasonic wave treatment; (DD) 1hour after ultrasonic wave treatment; (EE) 2 hour after ultrasonic wave treatment;

Description

Quantitative Estimating water-proofing quality of cosmetics goods by turbidity analysis}

The present invention relates to a method for quantitatively evaluating the water resistance to the skin of a makeup product that can be used as a means of quality assurance by objectively and numerically evaluating the water resistance.

Water-resistant cosmetics, such as mascara, have a thin film on the surface of the skin to prevent sweat or water from spreading or erasing to maintain the appearance of the initial makeup.

There is no quantitative method for evaluating the water resistance of makeup products, and a method of evaluating a subjective judgment after evaluating a certain number of panels has been widely used.

For example, in Korean Patent Publication No. 2002-83610, in order to evaluate the water resistance of the makeup cosmetics, after applying the skin color after soaking for 1 minute in water every 10 minutes every 10 minutes and dried to measure the degree of color change '◎' The water resistance was evaluated by the sensory evaluation of 'Δ' and '×'.

In addition, WO 2009/118106 discloses a water bath for 4 hours while stirring a glass plate at room temperature in order to measure the water resistance of cosmetics such as mascara containing a special polyurethane or its aqueous dispersion and a component that produces a cosmetic effect. After soaking in, the film area is measured, and the result is described as "coarse" or "excellent".

However, this method has a disadvantage in that the water resistance of the product is dependent on the subjective evaluation of the naked eye or the panel, so that it is not scientifically or objectively and can be subjectively determined by the tester.

Republic of Korea Patent Publication No. 2002-83610 International Publication No. 2009/118106

Accordingly, an object of the present invention is to provide a method for quantitatively evaluating the water resistance of a makeup product through turbidity analysis.

In order to achieve the above object,

Preparing a grooved film;

Drying after applying the makeup product on the film;

Sonicating the film to which the makeup product is applied for a predetermined time;

Injecting the sonicated film into a tube containing a buffer solution and heating to separate the makeup product from the film;

Centrifuging the solution in the tube through a centrifuge;

Taking the supernatant after the centrifugation and measuring turbidity with a haze meter; And

It provides a method for evaluating the water resistance of a makeup product comprising the step of objectively quantifying the water resistance of the makeup product by measuring the turbidity of the film coated with the makeup product before and after the ultrasonic treatment.

Through the quantitative evaluation method according to the present invention, the water resistance of the makeup product on the skin can be objectively and visually evaluated through the turbidity value, thereby increasing the reliability of the makeup product and greatly contributing as a means of quality assurance. There is.

1 is a flowchart showing a method for evaluating water resistance of a makeup product according to the present invention.
Figure 2 is a photograph showing the image according to the ultrasonic treatment time of the mascara test group and the control group.
3 is a graph showing the results of non-turbidity analysis.
Figure 4 is a photograph showing the state of the film after sonication, in order to measure the water resistance of the makeup base using a test group and two control groups A, B.
5 is a photograph showing the state of the film after sonication, in order to measure the water resistance of the lipstick using a test group and two control groups A and B.

The method of evaluating water resistance according to the present invention is not a sensory evaluation depending on a subjective judgment of a conventional subject, but a method of determining the degree of water resistance through quantitatively turbidity measurement. In particular, it evaluates the water resistance of makeup products applied to the film even under severe conditions such as ultrasonication, which not only provides higher reliability for water resistance evaluation but also allows an objective and visual evaluation of water resistance by turbidity values. Do.

Hereinafter, the present invention will be described in more detail with reference to the drawings.

1 is a flow chart showing a method for evaluating the water resistance of a makeup product according to the present invention.

Referring to Figure 1, first to provide a film having a certain size to apply a makeup product (S1). The film is a grooved film, the material is not particularly limited. In the experimental example of the present invention, a square pattern having a size of 2.0 × 2.0 cm 2 was used.

At this time, it is desirable to obtain the average value through 10 or more test groups in order to increase the reliability.

Next, after applying a certain amount of makeup product on the test site and dried (S2).

Makeup products can be used in all commercially available makeup products, makeup primer, foundation, makeup base, face powder, compact powder, twin cake, powder pact, powder foundation, mascara, lipstick, lip gloss, eyebrow pencil, eyeliner, eye Liner pencil, eye shadow, ball touch, lip liner, etc. are available.

At this time, the coating may vary depending on the area of the film to be used, and preferably, an amount of 0.05 to 0.1 g is applied per area of 20 × 20 cm 2. In the experimental example of the present invention, a makeup product was applied at a content of 0.1 g within a total area of 20 × 15 cm 2.

Means for the application include, but are not limited to, hand, stick, pomade, brush, bristled wand, sponge, pad, compression tube, liquid dispensing pump, and liquid dispensing pen. .

The drying is carried out at room temperature, specifically at 25 ± 3 ℃.

Next, the film coated with the makeup product on the surface is sonicated for a predetermined time (S3).

The ultrasonic treatment can be a device known in the art, for example, in the present invention, the 3510E-DTH, Branson, USA device was used. The ultrasonic treatment is preferably performed at a frequency of 40 kHz at 25 ± 0.5 ℃. In this case, the treatment time means an average time of life after application of the makeup product or a makeup correction time after the initial makeup.

At this time, the ultrasonic treatment time is performed within a maximum of 2 hours.

Next, the ultrasonicated film is injected into a tube containing a buffer solution and then heated to separate the applied makeup product from the film (S4).

The buffer solution uses a solution of pH 8.5 with 2% sodium dodecyl sulfate (SDS), 20 mM dithiothreitol (DTT), 5 mM ethylenediaminetetraacetic acid (EDTA) and 0.1 M Tris-HCl.

In addition, the tube uses an Eppendorf tube, where it may be selected to have a capacity of 0.5ml, 1.0ml, 1.5ml, 2ml, 5ml, 10ml, 15ml, 50ml and the like depending on the film size.

The heating is carried out at 90 ~ 100 ℃ for 10 minutes.

At this time, in the experimental example of the present invention, 1.5 ml of Eppendorf tubes containing 1 ml of buffer solution and finely sculpted films were treated before sonication, ultrasonic treatment for 1 hour, and treatment for 2 hours.

Next, the tube is mounted in a centrifuge to centrifuge the solution in which the makeup product is dissolved (S5).

The centrifugal separator may be a conventional centrifuge. In the experimental example of the present invention is carried out for 10 minutes at 10,000 ~ 20,000rpm, preferably 14,000rpm using a high-speed centrifuge (Micro 17TR, hanil, Korea).

Through such centrifugation, it is possible to completely separate the makeup product remaining on the film, and in particular, by taking the supernatant, it is possible to exclude other insoluble components in addition to the makeup product, thereby increasing the reliability of the test results.

Next, take the supernatant completely dissolved the makeup product through the centrifugation and measure the turbidity with a haze meter (S6).

That is, only the supernatant is transferred to a falcon tube, and then a buffer solution is added to measure the turbidity using a haze meter.

The volume of the buffer solution may vary depending on the volume of the cuvette of the haze meter. Since the minimum volume of the falcon tube of the haze meter (NDH 2000, denshoku, Japan) used in the experimental example of the present invention was 15 ml, 1 ml of the supernatant was taken and 14 ml of the buffer solution was added to measure turbidity.

Next, by measuring the turbidity of the film coated with the makeup product before and after the ultrasonic treatment to objectively quantify the water resistance of the makeup product (S7).

The image change of the makeup product before and after the ultrasound can be performed by paired t-test to confirm the water resistance of the makeup product to be tested.

Subsequently, the significant difference between the test group to be tested and the control group (an existing product or a product having a different composition) is performed by ANCOVA verification. As a result, objective quantitative evaluation is possible as not only the subjective judgment of the existing subject but also the small difference in the product or the product can be verified numerically so that it can be confirmed numerically.

In a preferred embodiment, the water resistance of the mascara, make-up base and lipstick of the make-up products was quantitatively analyzed according to the method proposed in the present invention. At this time, a 2.0 × 2.0 cm 2 square grooved film (20 × 15 cm 2) was provided, and 0.1 g of makeup products were applied thereto, followed by drying at room temperature for 30 minutes.

Subsequently, the film was sonicated for 1 or 2 hours under a 40 kHz condition using an ultrasonic device (3510E-DTH, Branson, USA). The sonicated film was separated into small pieces and separated into 1 ml Eppendorf tubes (1.5 ml, volume) with buffer solution. At this time, the film was used before the ultrasonic treatment, 1 hour treatment, and 2 hours treatment.

The eppendorf tube was heated at 100 ° C. for 10 minutes, and then centrifuged at 14,000 rpm for 10 minutes using a high speed centrifuge (Micro 17TR, hanil, Korea).

Then, only the supernatant was taken and transferred to a 15 ml Falcon tube, and 14 ml of buffer was added thereto. Next, the turbidity of each time point was measured using a haze meter (NDH 2000, denshoku, Japan).

Figure 2 is a photograph showing the image according to the ultrasonic treatment time of the mascara test group and the control group. Referring to FIG. 2, the area of the mascara-coated film is reduced by sonication, and the water resistance of the makeup product may be analyzed by changing the turbidity of each film.

Specifically, Table 1 and Figure 3 shows the results for the turbidity of the film for the ultrasonic treatment. The higher the turbidity value after sonication, the higher the water resistance.

Turbidity Analysis Results According to Ultrasonic Treatment Time I'm 1 hour 2 hours Test group 88.88 64.10 23.47 Control group 89.27 55.20 16.56 Significance (p <0.001) - 0.0000 0.0000 % Water resistance compared to the control - 37.49 11.16

Referring to Table 1 and FIG. 3, it was confirmed that there was a significant difference between the two products at the time point 1 and 2 hours after the ultrasonic treatment through ANCOVA verification.

Specifically, the test group can know the water resistance of the test group itself through the difference in turbidity before and after the ultrasonic treatment. In addition, it can be seen that the water resistance is improved by 11.16% after 2 hours when compared with the control group.

In addition, the longer the evaluation time, there is a problem that unnecessary variables will act, but if you shorten within two hours can reduce the problem, it can be seen that the reliability can be further increased if the optimal evaluation time is set to two hours.

It can be applied to other makeup products as well as mascara.

Figure 4 is a photograph showing the state of the film after sonication, in order to evaluate the water resistance of the makeup base using a test group and two controls A, B.

5 is a photograph showing the state of the film after sonication in order to evaluate the water resistance of the lipstick using a test group and two control groups A and B.

As shown in FIGS. 4 and 5, the turbidity of each makeup may be measured to confirm the water resistance of the makeup product, and the water resistance may be compared with other commercially available products or products of different compositions.

As described above, the analysis method according to the present invention can quantitatively analyze the water resistance of a makeup product. This makes it possible to objectively quantify water resistance, rather than observing minute differences in products or between products, depending on the subjective judgment of a conventional subject, thereby increasing the reliability of makeup products and greatly contributing as a means of quality assurance. It works.

The method according to the invention can be used as a method for measuring the reliability of makeup products.

Claims (10)

(S1) preparing a grooved film;
(S2) drying after applying the makeup product on the film;
(S3) sonicating the film to which the makeup product is applied for a predetermined time;
(S4) injecting the sonicated film into a tube containing a buffer solution and heating to separate the make-up product and the film;
(S5) centrifuging the solution in the tube through a centrifuge; And
(S6) taking the supernatant after the centrifugation and measuring the turbidity with a haze meter,
(S7) The water resistance evaluation method of the makeup product comprising the step of objectively quantifying the water resistance of the makeup product by measuring the turbidity of the film coated with the makeup product before and after the ultrasonic treatment. The method of claim 1, wherein the film has a groove made of a square of 2.0 × 2.0 cm 2. The method of claim 1, wherein the application is performed on an film per area of 20 × 20 cm 2. The water resistance evaluation method of the makeup product characterized by apply | coating in the quantity of 0.05-0.1g. The method of claim 1, wherein the drying is performed at 25 ± 3 ° C. The method of claim 1, wherein the ultrasonication is performed within 2 hours at a frequency of 40 kHz. The makeup product of claim 1 wherein the buffer solution is a solution of pH 8.5 with 2% sodium dodecyl sulfate (SDS), 20 mM dithiothreitol (DTT), 5 mM ethylenediaminetetraacetic acid (EDTA) and 0.1 M Tris-HCl. Method of evaluation of water resistance. The method of claim 1, wherein the heating is performed at 90 to 100 ° C. for 10 minutes. The method of claim 7, wherein the centrifugation is performed using a centrifuge for 10 minutes at 10,000-20,000 rpm. The method for evaluating the water resistance of a makeup product according to claim 1, wherein during the evaluation of water resistance, the turbidity change of the makeup product before and after the ultrasound is performed by a paired t-test. The method of claim 1, wherein the significant difference between the test group and the control group is evaluated by ANCOVA verification.

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