JP5068555B2 - Beauty method evaluation method - Google Patents

Beauty method evaluation method Download PDF

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JP5068555B2
JP5068555B2 JP2007027253A JP2007027253A JP5068555B2 JP 5068555 B2 JP5068555 B2 JP 5068555B2 JP 2007027253 A JP2007027253 A JP 2007027253A JP 2007027253 A JP2007027253 A JP 2007027253A JP 5068555 B2 JP5068555 B2 JP 5068555B2
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麻里子 江川
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Shiseido Co Ltd
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Description

本発明は美容方法の評価方法に係り、より詳細には、ラマン分光法により測定された皮膚内の水分量の深さ分布により導かれる指標に基づいて、化粧水を使用する際の美容方法の評価方法に関する。   The present invention relates to a method for evaluating a cosmetic method, and more specifically, based on an index derived from a depth distribution of moisture content in the skin measured by Raman spectroscopy, a cosmetic method when using a lotion. It relates to the evaluation method.

近年、健康や美容に関心が高まっており、特に、女性の肌の健康や美容に対する関心が非常に高くなってきている。しかしながら、美容方法は多種多様であり、さらに美容方法は人によって千差万別である。このような状況において、美容方法の効果を何らかの指標を用いて評価することができれば、効果的な美容をすることが期待できる。   In recent years, interest in health and beauty has increased, and in particular, interest in the health and beauty of women's skin has become very high. However, there are a wide variety of beauty methods, and the beauty methods vary from person to person. In such a situation, if the effect of the beauty method can be evaluated using some kind of index, it is possible to expect effective beauty.

特に、化粧水を使用する美容方法において、化粧水の提供側は、例えば、化粧水のコットン使用による皮膚への塗布を推奨しているが、論理的な根拠に裏付けられたものではなく、ヒトの官能評価によるものであり、その効果は明確ではなかった。さらに、使用する化粧水の推奨量や、皮膚への接触時間などは定まっておらず、使用後の皮膚の水分含有量を表すうるおい感は化粧水の使用者によってばらつきがあった。   In particular, in a cosmetic method using lotion, the lotion provider recommends, for example, the use of lotion cotton on the skin, but this is not supported by a logical basis. This was based on the sensory evaluation, and the effect was not clear. Furthermore, the recommended amount of lotion to be used, the contact time with the skin, etc. are not fixed, and the moist feeling representing the moisture content of the skin after use varies depending on the user of the lotion.

一方、従来から、皮膚内の水分量の深さ分布を測定する方法が知られており、例えば、ラマン分光法を用いて測定する方法が用いられている。(例えば、非特許文献1参照。)また、ラマン分光法は周知の技術であるため、皮膚内の水分量の深さ分布だけでなく、生物の細胞の組織内の成分を分析するためにも多様に活用されている(例えば、特許文献1参照。)。   On the other hand, conventionally, a method of measuring the depth distribution of the amount of water in the skin is known, and for example, a method of measuring using Raman spectroscopy is used. (For example, see Non-Patent Document 1.) Since Raman spectroscopy is a well-known technique, not only the depth distribution of the amount of water in the skin but also the analysis of components in the tissue of living cells. It is used in various ways (for example, see Patent Document 1).

しかしながら、化粧水を使用する美容方法と、皮膚の水分量の深さ分布との関係は、いまだかつて関連付けされておらず、ましてや、ラマン分光法により測定できる、水分量の深さ分布で美容方法を評価する方法はなかった。
特表2006−508358号公報 Caspers PJ, Lucassen GW, Bruining HA, Puppels GJ. Automateddepth-scanning confocal Raman microspectrometer for rapid in vivo determinationof water concentration profiles in human skin. J Raman Spectrosc 2000; 31:813-818
However, the relationship between the beauty method using lotion and the depth distribution of the moisture content of the skin has never been related, and moreover, the beauty method using the depth distribution of moisture content that can be measured by Raman spectroscopy. There was no way to evaluate.
JP-T-2006-508358 Caspers PJ, Lucassen GW, Bruining HA, Puppels GJ.Automateddepth-scanning confocal Raman microspectrometer for rapid in vivo determinationof water concentration profiles in human skin.J Raman Spectrosc 2000; 31: 813-818

したがって、本発明は上述に鑑みてなされたものであり、ラマン分光法により測定できる、皮膚の水分量の深さ分布を用いて得られる指標に基づく美容方法の評価方法を提供することを目的とする。   Therefore, the present invention has been made in view of the above, and an object of the present invention is to provide a method for evaluating a cosmetic method based on an index obtained using a depth distribution of moisture content of skin, which can be measured by Raman spectroscopy. To do.

本発明者は、ラマン分光法により測定される皮膚内の水分量の深さ分布を用いることで、美容方法を評価することができるという考えに基づき本発明を開発するに到った。   The present inventor has developed the present invention based on the idea that a cosmetic method can be evaluated by using the depth distribution of moisture content in the skin measured by Raman spectroscopy.

すなわち、本発明の美容方法の評価方法は、化粧水を用いる美容方法を評価する方法であって、該化粧水を塗布する前後皮膚内の水分量の深さ分布をラマン分光法を用いて測定する工程と、該化粧水を塗布する前後の皮膚内の水分量の深さ分布から、化粧水を塗布する前後の、単位角層相当厚あたりの皮膚の表面から角層相当厚の1/3までの積算水分量をく工程を有するIn other words, the evaluation method of the cosmetic method of the present invention is a method for evaluating a cosmetic method of using the lotion, the depth distribution of the amount of water in the skin before and after applying the lotion, using Raman spectroscopy and measuring Te, the lotion from the water content of the depth distribution of the skin before and after applying the, before and after applying the lotion, per equivalent thickness unit corneum stratum corneum equivalent thickness from the surface of the skin 1 / having 3 to the integrated water content rather guiding process.

また、本発明の美容方法の評価方法化粧水を用いる美容方法を評価する方法であって、該化粧水を塗布する前後の皮膚内の水分量の深さ分布を、ラマン分光法を用いて測定する工程と、該化粧水を塗布する前後の皮膚内の水分量の深さ分布から、化粧水を塗布する前後の、皮膚の表面からの深さが2μm、4μm及び6μmの3点における水分量の平均値を導く工程を有するFurther, the method for evaluating the cosmetic method of the present invention is a method for evaluating a cosmetic method using a lotion, and the depth distribution of the amount of moisture in the skin before and after applying the lotion is determined using Raman spectroscopy. And the depth distribution from the surface of the skin before and after applying the lotion at three points of 2 μm, 4 μm and 6 μm from the depth distribution of the moisture content in the skin before and after applying the lotion. A step of deriving an average value of moisture content .

本発明によれば、化粧水を使用する美容方法の効果を評価することができる。より具体的には、塗布前と塗布後において、例えば、化粧水マスク、パッティング、すべらせなど化粧水の塗布時間、塗布量などの美容法による、皮膚への効果を評価することができる。

According to the present invention, it is possible to evaluate the effects of cosmetic method for use of粧水. More specifically, after coating and prior to coating the fabric, for example, lotion mask, putting coating time lotion such as sliding, by cosmetic method such as coating amount, it is possible to evaluate the effect of the skin.

以下、本発明にしたがって実施した具体例を更に詳細に説明するが、本発明はこれらの実施例に限定されるものではない。なお、本発明の趣旨及び範囲を逸脱しない限り、その細部については様々な態様が可能である。   Specific examples carried out according to the present invention will be described in more detail below, but the present invention is not limited to these examples. In addition, various aspects are possible about the detail, unless it deviates from the meaning and range of this invention.

以下に添付図を参照しながら本発明を詳細に説明する。   Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

本発明の好ましい態様では、美容方法で使用する化粧品の塗布前後で皮膚内の水分量の深さ分布をラマン分光法によって測定し、その測定した値から導かれる指標に基づいて、化粧品の塗布前後で皮膚内の水分量の深さ分布を比較することによって美容方法の効果を評価する。   In a preferred embodiment of the present invention, the depth distribution of the moisture content in the skin is measured by Raman spectroscopy before and after the application of the cosmetic used in the cosmetic method, and based on the index derived from the measured value, before and after the application of the cosmetic. In order to evaluate the effect of the cosmetic method by comparing the depth distribution of the moisture content in the skin.

まず、ラマン分光法による皮膚内の水分量の深さ分布を測定する手法を説明する。   First, a method for measuring the depth distribution of moisture content in the skin by Raman spectroscopy will be described.

本発明で使用するラマン分光法による皮膚内の水分量の深さ分布の測定は、例えば、特許文献1に開示されているような機器及び方法を用いて行う。例えば、レーザー、ラマン・シグナルを測定するためのシグナル検出ユニット、および光ファイバー・プローブを含む機器を使用する。また、上記機器による測定方法は、ラマン・シグナルを生成し、光ファイバーを介してレーザー光を送り、光ファイバーを介してラマン・シグナルを受け、シグナル検出ユニットによってラマン・シグナルを検出することを含み、例えば、実質的に2500〜3700cm−1スペクトル領域にラマン・シグナルを有さないための光ファイバーを使用して、ラマン・シグナルを受けるものと同じ光ファイバーによってレーザー光を送り、シグナル検出ユニットで、上記スペクトル領域のラマン・シグナルを測定する。   The measurement of the depth distribution of the moisture content in the skin by Raman spectroscopy used in the present invention is performed using, for example, an apparatus and method as disclosed in Patent Document 1. For example, an instrument is used that includes a laser, a signal detection unit for measuring Raman signals, and a fiber optic probe. Further, the measurement method using the above apparatus includes generating a Raman signal, sending a laser beam through an optical fiber, receiving the Raman signal through an optical fiber, and detecting the Raman signal by a signal detection unit, for example, , Using an optical fiber for having substantially no Raman signal in the 2500-3700 cm-1 spectral region, sending the laser light through the same optical fiber that receives the Raman signal, Measure the Raman signal.

つまり、解析したい部位に対して光ファイバーを介してレーザー光を送り、そこから光ファイバーを通って散乱されたラマン・シグナルを受け、シグナル検出ユニットによってラマン・シグナルを検出する。   That is, a laser beam is sent to the part to be analyzed through the optical fiber, and the Raman signal scattered through the optical fiber is received from the laser beam, and the Raman signal is detected by the signal detection unit.

本発明では、上述した機器及び方法を用いて、皮膚内の水分量の深さ分布を測定するために、美容方法で使用する化粧品の塗布前後で皮膚表面から2μmごとに測定する。   In the present invention, in order to measure the depth distribution of the moisture content in the skin using the above-described apparatus and method, the measurement is performed every 2 μm from the skin surface before and after the application of the cosmetic used in the cosmetic method.

なお、ラマン分光法による測定は、周知の測定技術であるため、ここではこれ以上の詳細な説明はしない(ラマン分光法による測定の詳細は、特許文献1を参照。)。   Since measurement by Raman spectroscopy is a well-known measurement technique, further detailed description is not given here (refer to Patent Document 1 for details of measurement by Raman spectroscopy).

次に、本発明において、上述したラマン分光法による測定を用いた、美容方法の評価方法を具体的に説明する。   Next, in the present invention, a cosmetic method evaluation method using the above-described measurement by Raman spectroscopy will be specifically described.

本発明では、化粧品として、化粧水を用いる美容方法を評価する。より詳細には、化粧水の塗布前と塗布後で水分量の深さ分布をラマン分光法で測定して導かれる指標に基づき、皮膚内の水分量の深さ分布を比較することによって、化粧水の塗布の手法、化粧水の皮膚への接触時間、化粧水の使用量などを効果を評価する。   In this invention, the cosmetics method using a lotion is evaluated as cosmetics. More specifically, by comparing the depth distribution of moisture in the skin based on an index derived by measuring the depth distribution of moisture before and after applying lotion with Raman spectroscopy. Evaluate the effects of water application methods, skin contact time, and amount of skin water used.

図1は、本発明において、化粧水を用いる美容方法の一連の流れを説明する図である。   FIG. 1 is a diagram illustrating a series of flows of a cosmetic method using a lotion in the present invention.

まず、皮膚を石鹸で十分洗浄する。次いで、60分間放置した後で、洗浄後の皮膚の任意の部位で水分量の深さ分布を上述のラマン分光法によって測定した。その後、化粧水を皮膚に対して塗布するが、マスク、パッティング又はすべらせによって塗布した。なお、各塗布条件は、実施例によって異なり、それらの条件は下記の各実施例に詳述する。次に、化粧水を塗布した30秒後、ティッシュで当該部位の化粧水を軽く拭き取り、さらにその30秒後(つまり、化粧水を塗布した1分後)に、塗布前の測定部位と同じ部位の水分量の深さ分布をラマン分光法によって同様に測定した。さらにまた、化粧水を塗布した10分後において、同じ部位の水分量の深さ分布をラマン分光法によって測定した。   First, wash your skin thoroughly with soap. Subsequently, after leaving for 60 minutes, the depth distribution of the water content was measured by the above-mentioned Raman spectroscopy at an arbitrary part of the washed skin. Thereafter, lotion was applied to the skin, but applied by masking, putting or sliding. In addition, each application | coating condition changes with Examples, and those conditions are explained in full detail in each following Example. Next, 30 seconds after applying the lotion, gently wipe off the lotion at the site with a tissue, and then 30 seconds later (that is, 1 minute after applying the lotion), the same site as the measurement site before application The water depth distribution was similarly measured by Raman spectroscopy. Furthermore, the depth distribution of the water content at the same site was measured by Raman spectroscopy 10 minutes after applying the lotion.

本発明は、ラマン分光法によって測定した水分量の深さ分布を用いて得られる指標によって評価することを特徴とするが、例えば、本発明の美容方法の評価に用いる指標の一つに角層相当厚がある。図2のグラフに示すように、角層相当厚とは、上述のようにしてラマン分光法によって測定した、深さ2μmごとの数値をプロットして、曲線が「深さ」を示す横軸と平行になる時点での皮膚からの厚さとして定義する。具体的には、この角層相当厚に基づいて、皮膚内の水分量の深さ分布を化粧水の塗布前後で比較することによって、つまり、角層相当厚を塗布前後で比較することによって美容方法の効果を評価する。   The present invention is characterized in that it is evaluated by an index obtained using the depth distribution of the moisture content measured by Raman spectroscopy. For example, one of the indexes used for the evaluation of the beauty method of the present invention is a stratum corneum. There is considerable thickness. As shown in the graph of FIG. 2, the stratum corneum equivalent thickness is obtained by plotting numerical values every 2 μm depth measured by Raman spectroscopy as described above, and the horizontal axis where the curve indicates “depth”. Defined as the thickness from the skin at the time of parallelism. Specifically, based on this stratum corneum equivalent thickness, by comparing the depth distribution of the amount of moisture in the skin before and after the application of skin lotion, that is, by comparing the stratum corneum equivalent thickness before and after application. Evaluate the effectiveness of the method.

また、角層相当厚とは別の指標として、例えば、上部積算/角層相当厚を用いることもできる。上部積算/角層相当厚とは、単位角層厚あたりの角層相当厚の皮膚表面から1/3までの積算水分量を示す。また、別の指標として、表面3点平均水分量を用いることもできる。これは、ラマン分光法によって測定した水分量の深さ分布は、2μmごとにグラフにプロットされるため、皮膚表面から6μmまでの3点の水分量を平均したものである。   In addition, as an index different from the stratum corneum equivalent thickness, for example, the upper integrated / corneal layer equivalent thickness can be used. The upper integrated / stratified stratum corneum refers to the cumulative water content from the skin surface of the stratum corneum equivalent thickness to 1/3 per unit stratum corneum thickness. Further, as another index, the surface three-point average moisture content can also be used. This is because the depth distribution of the water content measured by Raman spectroscopy is plotted on a graph every 2 μm, so the water content at three points from the skin surface to 6 μm is averaged.

実施例
以下の実施例では、化粧水を用いた美容方法で、化粧水の塗布の手法、化粧水の皮膚への接触時間、および化粧水の使用量を変化させて、化粧水の塗布前と塗布後で皮膚内の水分量の深さ分布をラマン分光法によって測定し、上部積算/角層相当厚に基づいて評価した。
Examples In the following examples, a cosmetic method using lotion, the method of applying lotion, the contact time with the skin of lotion, and the amount of lotion used are changed before and after applying lotion. After application, the depth distribution of the moisture content in the skin was measured by Raman spectroscopy, and evaluated based on the upper integral / horny layer equivalent thickness.

実施例1
実施例1では、日常的に行っている4種類の化粧水の使用法を比較した。化粧水は、10%エタノール、5%1,3ブチレングリコールを含有するモデル化粧水を使用した。通常塗布としては、10.9μl/6cmの化粧水を手で30秒間塗布した。コットンを使用したすべらせにおいて、3ml/コットンで30秒間行った。コットンを使用したパッティングにおいて、3ml/コットンで2分間行った。コットンを使用したマスクにおいて、3ml/コットンで10分間行った。これらの条件で行った、化粧水の塗布前と化粧水の塗布後1分及び10分における皮膚内の水分量の深さ分布をラマン分光法によって測定した。その結果を図3に示す。
Example 1
In Example 1, the usage methods of four types of lotions used on a daily basis were compared. The skin lotion used was a model lotion containing 10% ethanol, 5% 1,3 butylene glycol. As a normal application, 10.9 μl / 6 cm 2 of lotion was applied by hand for 30 seconds. In sliding using cotton, 3 ml / cotton was performed for 30 seconds. In putting using cotton, it was performed at 3 ml / cotton for 2 minutes. In a mask using cotton, the treatment was performed at 3 ml / cotton for 10 minutes. Under these conditions, the depth distribution of the moisture content in the skin was measured by Raman spectroscopy before and after the application of the lotion. The result is shown in FIG.

ここでは、化粧水の皮膚に対する接触時間・塗布量などの美容方法の条件は異なるが、日常的に行っている美容方法の評価を目的として、上記の結果を図3のグラフとしてプロットした。日常の使用条件における美容方法では、上部積算/角層相当厚において、化粧水の塗布前後で水分量に差異が生じ、塗布後1分の場合、皮膚との接触時間が長い手法の効果が大きいことが評価できる。具体的には、肌にうるおい感をもたらすために、コットンマスクが最も効果が高く、次いで、コットンパッティング、コットンすべらせ、手の順に効果が高い評価となる。   Here, although the conditions of the cosmetic method such as the contact time and the amount of application to the skin of the skin lotion are different, the above results were plotted as a graph in FIG. 3 for the purpose of evaluating the daily cosmetic method. In the cosmetic method under daily use conditions, there is a difference in the amount of water before and after the application of the skin lotion in the upper integrated / horny layer equivalent thickness. Can be evaluated. Specifically, in order to bring a moist feeling to the skin, the cotton mask is the most effective, followed by cotton putting, cotton sliding, and hand evaluation.

さらに、以下の実施例では、最も効果が高かった、コットンマスクにおいて、化粧水の接触時間及び接触量を変えることによって評価した。   Furthermore, in the following examples, the cotton mask that was most effective was evaluated by changing the contact time and the contact amount of the skin lotion.

実施例2
実施例2では、化粧水の塗布方法をマスク、塗布量を3ml/コットンとして、接触時間を変えることで比較した。化粧水は、2%エタノール、3%グリセリンを含有するモデル化粧水を使用した。接触時間は、1、3、5、10、15及び20分として行い、化粧水の塗布前と化粧水の塗布後1分及び10分における皮膚内の水分量の深さ分布をラマン分光法によって測定した。
Example 2
In Example 2, the method of applying lotion was set as a mask, the amount applied was 3 ml / cotton, and comparison was made by changing the contact time. The skin lotion used was a model lotion containing 2% ethanol and 3% glycerin. The contact time is 1, 3, 5, 10, 15 and 20 minutes, and the depth distribution of the amount of moisture in the skin before and after applying the lotion is determined by Raman spectroscopy. It was measured.

図4は、実施例2の結果を示すものであり、ラマン分光法によって測定した皮膚内の水分量の深さ分布の値をグラフにプロットした。このグラフから、上述した上部積算/角層相当厚の定義にしたがって導かれる上部積算/角層相当厚において、化粧水の塗布前後における皮膚内の水分量の深さ分布を比較することによって評価することができる。化粧水の塗布前と塗布後を比べて、特に、塗布後1分では、図4のグラフから得られる上部積算/角層相当厚は、塗布前の水分量の深さ分布よりも大きく、その中でも、接触時間が長い方が水分の浸透量が増加している。これによって、化粧水を用いる美容方法の皮膚への効果を評価することができ、皮膚との接触時間が長い方が化粧水の肌への効果が高く、肌のうるおい感が高まったものと評価することができる。   FIG. 4 shows the results of Example 2, and the value of the depth distribution of the moisture content in the skin measured by Raman spectroscopy was plotted on a graph. From this graph, evaluation is made by comparing the depth distribution of the amount of moisture in the skin before and after the application of the skin lotion in the upper integrated / equivalent stratum corneum thickness derived according to the definition of the upper integrated / exposed stratum corneum described above. be able to. Compared before and after application of the skin lotion, especially at 1 minute after application, the upper integrated / corner layer equivalent thickness obtained from the graph of FIG. 4 is larger than the depth distribution of the amount of water before application. Among these, the longer the contact time, the greater the amount of moisture penetration. This makes it possible to evaluate the effect on the skin of a cosmetic method using lotion, and it is evaluated that the longer the contact time with the skin, the higher the effect on the skin of the lotion and the moist feeling of the skin has increased. can do.

実施例3
実施例3では、化粧水の塗布方法をコットンマスク、接触時間を20分として、化粧水の接触量を変えることで比較した。化粧水は、実施例1と同様、2%エタノール、3%グリセリンを含有するモデル化粧水を使用した。化粧水の接触量は、1、3及び5mlとして行い、化粧水の塗布前と化粧水の塗布後1分及び10分における皮膚内の水分量の深さ分布をラマン分光法によって測定した。その結果を図5に示し、ラマン分光法によって測定した水分量の深さ分布の値をグラフにプロットした。
Example 3
In Example 3, the method of applying the lotion was compared with a cotton mask, the contact time was 20 minutes, and the contact amount of the lotion was changed. As in Example 1, the skin lotion used was a model lotion containing 2% ethanol and 3% glycerin. The contact amount of the skin lotion was set to 1, 3 and 5 ml, and the depth distribution of the moisture content in the skin before and after the skin water application was measured by Raman spectroscopy. The result is shown in FIG. 5, and the value of the depth distribution of the water content measured by Raman spectroscopy was plotted on a graph.

図5から分かるように、上述した上部積算/角層相当厚の定義にしたがって導かれる上部積算/角層相当厚において、接触時間が20分の場合、化粧水の塗布前後で水分量に多大な差異が生じたが、塗布後の結果から、化粧水の使用量によっては、角層上部の水分増加量は変化しない傾向にあった。また、水分の浸透深さは増加傾向にあった。   As can be seen from FIG. 5, when the contact time is 20 minutes in the upper integration / corner layer equivalent thickness derived according to the definition of the upper integration / stratum layer equivalent thickness described above, the amount of moisture before and after application of the skin lotion is great. Although there was a difference, from the results after application, the amount of increase in water at the upper stratum corneum tended not to change depending on the amount of lotion used. Moreover, the water penetration depth tended to increase.

よって、上述した実施例1乃至3の結果から、化粧水を用いる美容方法において、皮膚との接触時間が長いほど、浸透効果が高く、肌の奥までうるおい感があることが評価できる。また、マスクの同一時間の比較では、角層上部の水分増加量の違いは少ないが、マスク時間が長いときは、浸透深さは若干上昇することが分かる。   Therefore, from the results of Examples 1 to 3 described above, it can be evaluated that in the beauty method using lotion, the longer the contact time with the skin, the higher the permeation effect and the moist feeling of the skin. Further, in the comparison of the same time of the mask, the difference in the amount of water increase in the upper stratum corneum is small, but it can be seen that the penetration depth slightly increases when the mask time is long.

したがって、本発明は、美容方法で使用する化粧品の塗布前後で皮膚内の水分量の深さ分布をラマン分光法によって測定し、その測定した値から導かれる指標に基づき、化粧品の塗布前後の皮膚内の水分量の深さ分布を比較することによって美容方法を評価することができる。   Therefore, the present invention measures the depth distribution of the moisture content in the skin before and after the application of the cosmetic used in the cosmetic method by Raman spectroscopy, and based on the index derived from the measured value, the skin before and after the cosmetic application The beauty method can be evaluated by comparing the depth distribution of the amount of water in the inside.

以上本発明の好ましい実施例について詳述したが、本発明はかかる特定の実施形態に限定されるものではなく、特許請求の範囲に記載された本発明の趣旨の範囲内において、種々の変形・変更が可能である。   The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to such specific embodiments, and various modifications and changes can be made within the scope of the gist of the present invention described in the claims. It can be changed.

本発明における、化粧水を用いる美容方法の一連の流れを説明する図である。It is a figure explaining a series of flows of the beauty method using a lotion in the present invention. 本発明において、ラマン分光法によって測定した皮膚内の水分量の深さ分布を用いて得られる指標の一例を示す図である。In this invention, it is a figure which shows an example of the parameter | index obtained using the depth distribution of the moisture content in the skin measured by the Raman spectroscopy. 日常の使用条件における美容方法によって、本発明の美容方法の評価方法を説明する図である。It is a figure explaining the evaluation method of the beauty method of this invention by the beauty method in daily use conditions. 実施例2における本発明の美容方法の評価方法を説明する図である。It is a figure explaining the evaluation method of the beauty method of this invention in Example 2. FIG. 実施例3における本発明の美容方法の評価方法を説明する図である。It is a figure explaining the evaluation method of the beauty method of this invention in Example 3. FIG.

Claims (2)

化粧水を用いる美容方法評価する方法であって、
該化粧水を塗布する前後皮膚内の水分量の深さ分布をラマン分光法を用いて測定する工程と
化粧水を塗布する前後の皮膚内の水分量の深さ分布から、化粧水を塗布する前後の、単位角層相当厚あたりの皮膚の表面から角層相当厚の1/3までの積算水分量をく工程を有することを特徴とする美容方法の評価方法。
A method for evaluating a cosmetic method using lotion ,
The depth distribution of the amount of water in the skin before and after applying the lotion, and measuring using Raman spectroscopy,
From the depth distribution of the amount of moisture in the skin before and after applying the skin lotion, the cumulative moisture from the skin surface per unit stratum corneum equivalent thickness to 1/3 of the stratum corneum equivalent thickness before and after applying the skin lotion evaluation method of cosmetic process, characterized in that it comprises an amount of rather guiding process.
化粧水を用いる美容方法を評価する方法であって、  A method for evaluating a cosmetic method using lotion,
該化粧水を塗布する前後の皮膚内の水分量の深さ分布を、ラマン分光法を用いて測定する工程と、  Measuring the depth distribution of the moisture content in the skin before and after applying the skin lotion using Raman spectroscopy;
該化粧水を塗布する前後の皮膚内の水分量の深さ分布から、化粧水を塗布する前後の、皮膚の表面からの深さが2μm、4μm及び6μmの3点における水分量の平均値を導く工程を有することを特徴とする美容方法の評価方法。  From the depth distribution of the moisture content in the skin before and after applying the lotion, the average value of the moisture content at three points where the depth from the surface of the skin before and after applying the lotion is 2 μm, 4 μm and 6 μm is calculated. A method for evaluating a cosmetic method, comprising a step of guiding.
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