KR20120140111A - Agricultural pesticide against plant disease containing solution typed thiamine di-lauryl sulfate - Google Patents

Abstract

PURPOSE: An agrochemical containing thiamine di-lauryl sulfate of a solution form for preventing plant diseases is provided to enhance antifungal activity and to maintain stable solution state. CONSTITUTION: A method for manufacturing an agrochemical for preventing plant diseases comprises: a step of adding thiamine di-lauryl sulfate(TDS) powder to 50-95% ethanol and stirring to prepare a TDS ethanol solution; a step of adding water to the TDS ethanol solution and stirring to prepare a TDS solution; and a step of adding TDS solution as an active ingredient. The agrochemical contains TDS solution as an active ingredient. The plant diseases are caused by pathogen of Botrytis cinerea, Colletotrichum gloeosporioides, Sclerotinia sclerotiorum, or Phytopthora infestans. [Reference numerals] (AA) TDS solution; (BB) D.W; (CC) TDS powder solution

Description

Agricultural pesticide against plant disease containing solution typed thiamine di-lauryl sulfate}

The present invention relates to a pesticide for controlling and preventing plant diseases, and more particularly, containing thiamine di-lauryl sulfate (TDS) in the form of an aqueous solution prepared by dissolving in ethanol and diluting in water. It relates to a pesticide for plant disease control and prevention.

As modern society enters, the rapid growth of the population and the shrinking agricultural sector are increasing the importance of food production all over the world, and much investment and development is being made in the agricultural sector to improve agricultural productivity. As one of these efforts, pesticides have been used to increase plant productivity, but organic synthetic pesticides, which have been used until now, have caused environmental pollution such as pesticide poisoning, groundwater and soil pollution due to indiscriminate abuse.

However, what is more problematic is the consumer's anxiety and rejection of the stability of the plant caused by this. In order to solve this problem, the development of biopesticides that can safely and efficiently inhibit plant diseases has been studied in recent years.

Meanwhile, vitamin B ₁ extracted from rice bran is _one of the earliest known vitamins and is synthesized by green plants or microorganisms, and is contained in rice bran, embryos, yeast, and the like. Vitamin B ₁ is also found in animal nerves, and has been considered as one of the alternative natural pesticides known to activate plant self-defense genes and contribute to growth. However, since vitamin B ₁ has a problem in thermal stability, it is likely to be lost in the heat treatment process, and there is a problem in that the stability in water is low.

Therefore, there is a side that is difficult to use as a pesticide to prepare the vitamin B ₁ in an aqueous solution state, there is also a problem to solve the problem of loss and denaturation of vitamin B ₁ due to process treatment.

In order to solve the above problems with vitamin B ₁ , the present invention is to develop and provide a method for preparing a new type of vitamin B _1- containing pesticides with high thermal stability and stability in water.

In order to achieve the above object, the present invention provides a TDS ethanol solution by adding thiamine di-lauryl slfate (TDS) powder to 50-95% ethanol and stirring it in the first embodiment of the present invention. Manufacturing; Adding water to the TDS ethanol solution and then stirring to prepare a TDS aqueous solution; And adding the TDS aqueous solution as an active ingredient. The method provides a method of preparing a pesticide for plant disease control and prevention, comprising: a.

Thiamine di-lauryl sulfate (TDS), a vitamin B ₁ derivative, is known to inhibit the infection of fungi, bacteria and viruses that infect foods. However, there are many problems in terms of industrial applications due to low thermal stability and low stability in water. In order to solve this problem, it is necessary to develop vitamin B ₁ or a derivative thereof having high thermal stability and stability in water.

In the present invention, by dissolving thiamine di-lauryl sulfate (TDS) in ethanol, and then diluted in water to prepare an aqueous solution form, a stable state in the aqueous solution was secured, the powder of the existing vitamin B ₁ water It was confirmed that the effect of controlling against plant pathogens could be enhanced rather than simple dilution.

On the other hand, in the method of producing a pesticide for controlling and preventing plant diseases of the present invention, the thiamine di-lauryl sulfate (TDS) is, for example, an aqueous thiamine hydrochloride (thiamine hydrochloride) solution and sodium lauryl The aqueous solution of sodium lauryl sulfate may be prepared by mixing and reacting at room temperature, followed by crystallization.

On the other hand, the present invention in the second aspect of the present invention, the thiamine di-lauryl sulfate (thiamine di-lauryl sulfate (TDS) powder) is added to 50 ~ 95% ethanol, followed by stirring to prepare a TDS ethanol solution ; And, after adding water to the TDS ethanol solution, the step of stirring; provides a pesticide for plant disease control and prevention, characterized in that containing the aqueous solution of TDS prepared from the active ingredient.

The pesticide of the present invention is applicable to both monocot and dicotyledonous plants including rice and vegetable crops such as pepper, cucumber, ginseng, lettuce, potatoes and tomatoes.

The pesticide of the present invention may be mixed with other conventional synthetic pesticides or biopesticides or mixed with fertilizers.

On the other hand, in the pesticides for controlling and preventing plant diseases of the present invention, the pesticides for controlling and preventing plant diseases may contain 1 to 1,000 ppm of thiamine di-lauryl sulfate (TDS).

On the other hand, in the pesticide for controlling and preventing plant diseases of the present invention, the plant diseases are, for example, Botrytis cineraria ( Botrytis cinerea ), Colletotrichum-Colletotrichum gloeosporioides ), Sclerotinia sclerotiorum and Phytopthora infestans infestans ) may be caused by any one of the pathogens selected.

On the other hand, the present invention is a third aspect of the present invention, in which a pesticide containing an aqueous solution of thiamine di-lauryl sulfate (TDS) prepared by the method described in the first aspect of the present invention is sprayed onto a plant. It provides a plant disease control method characterized in that.

The pesticide throughput in the present invention does not deviate significantly from the existing throughput or handles in a smaller amount range. In addition, it was confirmed that the treatment at any stage of growth from the seed of the plant to the maturity stage did not affect the germination or growth of the plant at concentrations below the concentration range, and there was no visible weakness until 10 days after the treatment. TDS, a derivative of vitamin B ₁ of the present invention, can be treated by conventional pharmaceutical treatment methods such as spraying, irrigation or dipping.

In the present invention, the plant to be applied may be any one selected from, for example, pepper, cucumber, ginseng, lettuce, potato and tomato.

Thiamine di-lauryl sulfate (TDS) aqueous solution prepared by the method of the present invention was maintained in a stable state, and further enhanced antifungal activity than using di-lauryl sulfate (TDS) powder in simple dilution with water. Indicated.

Pesticides in aqueous solution containing di-lauryl sulfate (TDS) according to the present invention solves the disadvantages of organic synthetic pesticides, which are a problem recently, and environmental pollution, such as groundwater and soil, plants in a nontoxic and safe state Direct growth inhibitory effects on pathogenic pathogens. In addition, the pesticide of the present invention has the advantage that it can also express the autoimmune and defense mechanisms inherent in vitamin B ₁ because the active ingredient is a vitamin B ₁ derivative.

Therefore, the present invention will ultimately be able to make a great contribution to the eco-friendly organic materials and pesticide industry necessary for eco-friendly agricultural production.

1 is a photograph of a thiamine di-lauryl sulfate (TDS) aqueous solution prepared through the present invention.
2 is a result of solubility measurement of the aqueous thiamine di-lauryl sulfate (TDS) solution prepared in the present invention. In FIG. 2, the 'DW' sample is distilled water as a control sample, and the 'TDS powder solution' sample is a solution obtained by simply adding thiamine di-lauryl sulfate powder to distilled water, and the 'TDS aqueous solution' sample is prepared in the present invention. TDS aqueous solution sample.
FIG. 3 shows the results of antifungal activity of a sample of simply diluted thiamine di-lauryl sulfate (TDS) powder and a sample of aqueous thiamin di-lauryl sulfate (TDS) solution. 'a' is a control (untreated) sample, 'b' is 100 ppm sample of TDS powder, 'c' is 50 ppm sample of aqueous TDS solution, and 'd' is 100 ppm sample of aqueous TDS solution.
4 is a result of measuring the mycelial growth inhibition rate of the sample obtained by simply diluting the thiamine di-lauryl sulfate (TDS) powder and the sample of the aqueous thiamine di-lauryl sulfate (TDS) solution. 'A' is a control (untreated) sample, 'B' is 100 ppm sample of TDS powder, 'C' is 50 ppm sample of aqueous TDS solution, and 'D' is 100 ppm sample of aqueous TDS solution.
FIG. 5 is a photograph of a mycelia of a thiamine di-lauryl sulfate (TDS) aqueous solution showing antifungal activity.

Hereinafter, the content of the present invention will be described in more detail through the following examples and experimental examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

Example  1: thiamine die - Lauryl Sulfate ( thiamine di - lauryl  sulfate: TDS A) powder manufacturing

TDS used in the present invention is reacted and crystallized by mixing thiamine hydrochloride (Sigma, St. Louis, MO, USA) and sodium lauryl sulfate (Sigma, St. Louis, MO, USA) It was prepared by.

First, 100 to 900 g of thiamine hydrochloride (TH) was added to 1 L of distilled water and dissolved at room temperature. Next, 100-900 g of sodium lauryl sulfate (SLS) was added to 3 L of distilled water and dissolved at room temperature. Dissolved TH and SLS were collected in 0.2 to 5 μm filters ACHEREY-NAGEL and Neumarn-Neander Str. Germany), and each solution was reacted by stirring at a reaction temperature of 30 to 50 ° C for 1 hour.

After 2 hours of cooling reaction at a low temperature of 0 ~ 10 ℃ for crystallization of the reaction solution, centrifuged twice in a high-speed centrifuge set at a temperature of 2000 rpm, 0 ~ 10 ℃ Proceeded.

The centrifuged sample was vacuum dried at a temperature of 20 to 40 ° C. for 3 hours in a vacuum dryer, and then ground to prepare a TDS powder of 20 mesh.

Example  2: thiamine die - Lauryl Sulfate ( TDS Aqueous Solution Preparation

100 to 900 g of the TDS powder prepared above was dissolved in 1 L of 50 to 95% ethanol, and then stirred for about 10 to 20 minutes until the TDS powder was completely dissolved. Thereafter, 20 mL of a TDS ethanol solution completely dissolved in 50-95% ethanol was added to 9,980 mL of distilled water, followed by stirring for 1 hour to prepare 1 L of an aqueous TDS solution of the present invention.

Experimental Example  1: thiamine die - Lauryl Sulfate ( TDS ) Solubility Measurement of Aqueous Solution

It was to observe whether there is no material precipitated from the TDS aqueous solution prepared in the above Example.

The magnetic bar was stirred for 1 hour at a stirring speed of 800 rpm. 1 is a diagram showing the dissolution of the TDS aqueous solution after stirring for 1 hour. As shown in FIG. 1, a very transparent solution state can be seen, which means that thiamine hydrochloride and sodium lauryl sulfate are completely reacted and there is no remaining precipitate material.

On the other hand, in order to determine the degree of dissolution in detail, the solubility of the aqueous solution diluted by simply adding the TDS powder to the TDS aqueous solution prepared in the present invention and distilled water at the same concentration was measured and compared.

After each sample was placed for 6 hours to stabilize the solution, 200 μl each was added to a 96 well plate, and the absorbance was measured using an ELISA reader at a specific wavelength of 230 nm.

Figure 2 is the result of measuring the solubility of the TDS aqueous solution and TDS powder diluent. As a result of the measurement, the TDS aqueous solution of the present invention was similar to the absorbance of distilled water, and all TDS powders were dissolved to show transparency similar to that of distilled water, whereas the sample dissolved by simply adding TDS powder set in another experimental group to distilled water was concentrated. The absorbance increased with, indicating that the TDS powder was suspended in solution and not suspended.

Experimental Example  2: Plant disease  Thiamine against pathogens die - Lauryl Of sulfate (TDS)  Antifungal Activity Measurement

 1) Crop pathogens used to measure antifungal activity

Table 1 Crop Disease and Pathogens Used to Measure Antifungal Activity

Pathogens causing the four crop diseases shown in Table 1 were used to measure the antifungal activity of the present experimental example, PDA (Potato Dextrose Agar) medium was used as the antifungal activity evaluation medium, 30 ℃, cultured in cancer conditions It was. Plant pathogens used in the present invention, anthrax, colletotrihum gloeosporioides , Sclerotinia sclerotiorum , a bacterium, phytophthora infestan Sphytopthora infestans ), and The ash fungus, Botrytis cinerea , was sold by KACC.

 2) How to measure antifungal activity

The control group used to measure antifungal activity was the untreated group (a), and the experimental group was prepared by simply dissolving thiamin di-lauryl sulfate (TDS) in 100 ppm of purified water (b) and thiamine di-lauryl sulfate ( TDS) 50 ppm sample (c) and 100 ppm sample (d) in aqueous solution.

TDS prepared for each concentration was added together in the preparation of PDA medium. Each pathogen was cut into 0.5 cm in diameter on the medium containing TDS and placed in the middle of PDA medium. After inoculation, it was observed whether the mycelia continued to grow out of Petri dishes.

 3) Antifungal activity measurement

Figure 3 shows the growth of each mold on the PDA medium containing vitamin B ₁ aqueous solution by concentration. In all four types of fungi, mycelial growth was suppressed in all samples compared to the control group. Especially, the samples prepared by the aqueous solution by the method of the present invention were much better than the samples prepared by simply diluting the TDS powder. Indicated.

In order to measure the more detailed antifungal activity, the colony growth was measured by comparing the diameter of the colonies. The mycelial growth inhibition rate was measured by the following formula, and the result was as shown in FIG. 4.

Equation 1 mycelial growth inhibition rate

As a result of the measurement, Sclerotinia first The inhibition of mycelial growth of sclerotiorum ) was measured at 22.4%, the lowest inhibition rate in 100 ppm of TDS powder compared to the control, and the highest inhibition rate was measured with 100 ppm aqueous TDS solution with 55.5% inhibition rate.

In addition, the inhibition rate of the 50 ppm TDS aqueous solution, which is lower than the highest concentration of 100 ppm TDS powder, resulted in 16% higher inhibition. This makes Sclerotinia more efficient, even at lower concentrations, when producing and using aqueous TDS solutions than using TDS powders. sclerotiorum ) means that the growth can be suppressed.

Next, the inhibition of mycelial growth against Colletotrichum gloeosporioides was also measured as 29.3%, the lowest inhibition rate in 100 ppm of TDS powder compared to the control, and the highest inhibition rate was 74.0%. Measured with 100 ppm aqueous TDS solution. In addition, the inhibition rate of the TDS aqueous solution at 50 ppm concentration, which is lower than the highest concentration of 100 ppm TDS powder, was 65.7%, which was 30% higher than the TDS powder sample. This also means that it is possible to inhibit the growth of Colletotrichum gloeosporioides more efficiently with a small concentration of content.

Next, mycelial growth inhibition rate of Phytopthora infestans was 19.6% in 100 ppm TDS powder, 33.8% in 50 ppm TDS solution and 62.7% in 100 ppm TDS solution. The highest inhibition rate was shown in the TDS aqueous solution at ppm concentration. In addition, the inhibition rate of the 50 ppm concentration TDS aqueous solution, which is lower than the highest concentration of 100 ppm TDS powder, was more than doubled. This means that it is possible to more effectively inhibit the growth of Phytopthora infestans when the TDS aqueous solution is prepared and used than the TDS powder like other pathogens described above.

Finally, the mycelial growth inhibition rate of Botrytis cinerea was the lowest as 11.2% at 100 ppm of TDS powder, 33.4% at 50 ppm of TDS solution, and 100 ppm of TDS solution. Inhibition rate of 44.6% was shown. These results indicate that, as with other pathogens, the preparation and use of an aqueous solution of TDS shows a superior inhibitory effect on Botrytis cinerea than the use of TDS powder.

 4) Observation of Pathogens

When the aqueous thiamine di-lauryl sulfate (TDS) solution has antifungal activity, the mycelia of pathogens do not grow, resulting in a smaller size of mycelial growth than the control group. At this time, the hyphae and the rounded portion of the circular shape is taken to look at the microscope, the death form of the hyphae can be observed.

Figure 5 shows a typical Sclerotinia sclerotinia sclerotiorum ) and colletotric dance Colletotrichum gloeosporioides ) is a picture of the hyphae and the mycelia are inhibited by the growth of thiamine di-lauryl sulfate (TDS) solution.

As shown in FIG. 5, the general mycelia are constantly growing without being disturbed, and the mycelia that have been inhibited from mycelial growth by aqueous thiamin di-lauryl sulfate (TDS) solution are thiamine di-lauryl sulfate (TDS). ) Adhered to the cell wall of the mycelia, inhibiting the growth of the mycelia.

These results may mean that the aqueous solution of TDS may increase the persistence of TDS remaining on the cell wall of the mycelium, increase the surface area acting on the mycelium, thereby exhibiting excellent antifungal activity even at a small concentration.

Claims (7)

Adding thiamin di-lauryl slfate (TDS) powder to 50-95% ethanol, followed by stirring to prepare a TDS ethanol solution;
Adding water to the TDS ethanol solution and then stirring to prepare a TDS aqueous solution; And
Adding the aqueous TDS solution as an active ingredient; Plant disease control method for preventing and preventing plant diseases comprising the.
The method of claim 1,
The thiamine di-lauryl sulfate (TDS) is,
Thiamine hydrochloride (thiamine hydrochloride) aqueous solution and sodium lauryl sulfate (sodium lauryl sulfate) aqueous solution by mixing and reacting at room temperature, the method of producing a pesticide for preventing and preventing plant diseases, characterized in that it was prepared by crystallization.
Adding thiamin di-lauryl sulfate (TDS) powder to 50-95% ethanol and then stirring to prepare a TDS ethanol solution; And,
After adding water to the TDS ethanol solution, the step of stirring; Plant pest control and preventive pesticides, characterized in that containing the aqueous solution of TDS prepared from as an active ingredient.
The method of claim 3,
The plant disease control and prevention pesticides,
A pesticide for plant disease control and prevention, comprising 1 to 1,000 ppm of thiamine di-lauryl sulfate (TDS).

The method of claim 3,
The plant disease,
Botrytis Ceria cinerea ), Colletotrichum-Colletotrichum gloeosporioides ), Sclerotinia sclerotinia Sclerotiorum ) and Phytopthora infestans ( Phytopthora infestans ) is a pesticide for plant disease control and prevention, characterized in that caused by any one of the pathogens selected.

A plant disease control method comprising spraying a plant containing an aqueous solution of thiamine di-lauryl sulfate (TDS) prepared by the method of claim 3.
The method according to claim 6,
The plant,
Plant disease control method, characterized in that any one selected from pepper, cucumber, ginseng, lettuce, potatoes and tomatoes

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