KR20120134264A - Food additive composition for preventing or improving diabete comprising purple bamboo salt - Google Patents

Abstract

PURPOSE: A food additive composition containing purple crystal-like bamboo salt for treating and preventing diabetes is provided to use the composition with a low diabetogenic effect instead of salt. CONSTITUTION: A food additive composition for treating and preventing diabetes contains purple crystal-like bamboo salt as an active ingredient. The purple crystal-like bamboo salt is obtained by the following steps: filling bay salt in a bamboo container, and sealing the container; firstly heating the sealed bay salt at 500-850 deg. C for 1-10 hours; crushing the heated bay salt for 8 times; mixing the firstly heated bay salt with bamboo; and secondly heating the mixture after inserting into the bamboo container at 1,000-2,000 deg. C for 1-10 hours.

Description

FOOD ADDITIVE COMPOSITION FOR PREVENTING OR IMPROVING DIABETE COMPRISING PURPLE BAMBOO SALT}

The present invention relates to a food additive composition.

Insulin is a hormone that regulates blood sugar, and when the concentration of glucose in the blood is increased, insulin secretion is increased in the pancreas and glucose is introduced into the tissue to maintain a certain concentration of glucose in the blood.

In normal people, the body's insulin sensitivity (effect) and insulin secretion are balanced so that blood sugar is always regulated within the normal range. However, when insulin is deficient or susceptible to susceptibility, blood sugar cannot be used to increase the concentration of insulin in the blood. In addition, the body's susceptibility to insulin is reduced, increasing insulin resistance. The insulin resistance leads to a weak state promoting the absorption of sugar, which is the main action of insulin in skeletal muscle, adipocytes or liver. Due to the insulin resistance, even if the amount of insulin in the body is about the same, the blood glucose lowering action is weakened, and thus, more insulin is required to maintain the blood sugar normal. Accordingly, the phenomenon in which a large amount of insulin is secreted into the body to supplement insulin resistance is called hyperinsulinemia. In addition, excessive secretion of insulin increases appetite and promotes fat synthesis as well as inhibits lipolysis, thereby inducing obesity.

When the balance between insulin sensitivity and insulin secretion in the body is broken, that is, insulin deficiency or insulin sensitivity is lowered, the glucose metabolism abnormality disease that causes glucose to be discharged in the urine due to the inability to use blood sugar is not called diabetes mellitus. do.

The above-mentioned diabetes is largely classified into type 1 diabetes and type 2 diabetes.

The type 1 diabetes, namely insulin-dependent diabetes, is caused by decreased insulin production and secretion due to damage of pancreatic beta cells (β-cells) that secrete insulin. In order to treat type 1 diabetes, insulin is usually administered externally to lower blood glucose levels. Type 2 diabetes, that is, insulin-independent diabetes, refers to a state in which blood sugar levels are higher than normal, and occurs when the balance of insulin secretion and the secretion of insulin from the pancreas is broken in peripheral tissues such as muscle, fat, and liver. . Most diabetics show insulin resistance and hyperinsulinemia before progressing to type 2 diabetes. In other words, type 2 diabetes lowers insulin action to lower the initial blood glucose level, does not effectively absorb the sugar in the peripheral tissue, and can not be used as an energy source of insulin resistance, resulting in lowering of hyperglycemia to increase insulin secretion Hyperinsulinemia. If you do not overcome this, you will eventually go on to type 2 diabetes.

The World Health Organization (WHO) recently reported that the number of diabetics worldwide will be about 150 million in 2000 and about 220 million in 2010. In Korea, due to the inappropriate lifestyle and the increase of obese population, the number of diabetics is currently about 4 million, accounting for about 10% of the total population, and the number of patients with type 2 diabetes has been steadily increasing in recent 10 years. to be.

Inappropriate lifestyles have been suggested in connection with the causes of diabetes, particularly type 2 diabetes. In particular, Korea has been reported to cause insulin resistance due to excessive salt intake, causing insulin resistance. According to a related study, sodium chloride (NaCl), the main component of the salt, has been found to be a direct cause of high blood pressure and diabetes. For this reason, WHO / ISH recommends adult daily salt intakes of less than 5 g, and the Japanese Society of Hypertension in 2000 recommends that adults consume less than 7 g in relation to the daily intake of salt. Doing.

Sodium chloride (NaCl), a major component of salt, is physiologically indispensable for animals. Specifically, sodium chloride adjusts the osmotic pressure of body fluids, sodium, a component of sodium chloride, binds with phosphoric acid, maintains the acid group parallel to the body fluid as a buffer, and constitutes an alkaline digestive fluid such as bile, interest, or intestinal fluid, and nerves. Acts as a stimulant delivery substance, and chlorine, another component of sodium chloride, constitutes gastric juice.

Salt used as a seasoning for flavoring foods in daily life is a component that is closely related to the physiological activity and life support of the animal as described above, if the lack of salt intake in the short term, the secretion of digestive fluid is insufficient Loss of appetite occurs, and in the long term, systemic weakness, boredom, fatigue or mental anxiety occur, and sudden loss of salinity in the body causes obvious physical and mental impairment such as dizziness, insufficiency, loss of consciousness, and dysfunction.

The salts can be broadly classified into rock salt, refined salt, recontaminated salt, and sun salt, and are known to have different mineral contents depending on the type of salt. In other words, rock salts and refined salts, which are consumed mostly by people around the world, contain more than 99% of sodium chloride, while there are few minerals such as K, Ca, and Mg. Natural salts, especially Korean-made tidal flat salts, contain 85% of sodium chloride. On the other hand, it is reported to contain a large amount of mineral components such as K, Ca or Mg.

Recently, the tidal flat salt inhibits blood pressure rise and inhibits the expression of insulin receptor substrate-1 serine phosphorylation enzyme, which is a negative regulator in insulin signaling, compared to purified salt, which is a salt that is almost free of minerals. It has also been reported to further reduce insulin resistance by increasing glucose uptake in fat or muscle tissue. From the above results, it was suggested that not only the intake of salt but also the type of salt affects the relationship between salt and diabetes.

As described above, salt must be ingested not only for the purpose of seasoning but also for health reasons, but in case of excessive intake, it causes diabetes. There is a need for the development of food additives that can suppress or improve the diabetes-inducing effects of salt, such as prevention. In this regard, it is necessary to develop a food additive having a prevention or improvement effect on diabetes by using a salt with a natural biological material known to have a prevention or improvement effect on diabetes.

The present invention is to improve the problems of the prior art as described above, foods containing a natural substance with a significantly lower diabetes-causing effect as compared to salts, in particular tablet salts, which can be given salty taste to foods, in particular, as an active ingredient It is an object to provide an additive composition.

In order to achieve the above object, the present invention provides a food additive composition comprising a porridge salt as an active ingredient. Although the food additive composition may be used together with salt to impart salty taste, the food additive composition may be a food additive composition having an improved or preventive effect on diabetes, that is, a significantly lower diabetes-inducing effect or a diabetic improvement effect. In this aspect, the food additive composition may be a food substitute composition for salt replacement, preferably a food additive composition for tablet salt replacement.

In addition, the present invention provides a method for producing suicidal salts having an effect of improving or preventing diabetes, that is, a diabetes, a diabetes lowering effect is significantly lower than a salt, preferably a purified salt.

The inventors of the present invention, while studying insulin sensitizers derived from natural substances that do not have side effects such as weight gain and have no problems with resistance or stability even after long-term use, glucose tolerance test, glucose count in fat cells and muscles As a result of the glucose uptake test, the expression level of insulin-related protein in adipose tissue and the measurement of lipid peroxide in the blood, it was reported that porphylitis compared with the sun salt, which has a significantly lower antidiabetic effect, that is, diabetes induction than tablet salt It confirmed that the antidiabetic effect was excellent, and completed this invention.

Hereinafter, the present invention will be described in more detail.

In the present invention, salt means all types of salt that can be used for food, not for industrial purposes, and include salt, rock salt, flower salt, roasted salt, bamboo salt, refined salt, reconstituted salt, molten salt, refined salt or processed salt, and the like. do.

In the present invention, the natural salt refers to an unprocessed salt made by concentrating seawater by evaporating water using natural forces such as wind and sunlight after introducing seawater or seawater into a reservoir or salt field, and having coarse and translucent hexagons. Has the characteristic of having a crystal. The sun salt is called mineral salt because it is rich in many minerals (minerals) such as calcium, magnesium, zinc, potassium, and iron. In particular, the natural salt produced in our country, specifically, the natural salt produced in Jeollanam-do is preferred because it is rich in minerals.

In the present invention, bamboo salt means salt baked in a bamboo barrel. In addition, the porridge salt means a salt of purple or bluish color among the salts of the bamboo salt, roasted with bamboo salt in bamboo.

In the present invention, a functional food is a biological defense rhythm control or disease prevention of a food group or a food composition that has added value to the food by using physical, biochemical, or biotechnological techniques to act and express the function of the food for a specific purpose. Means foods that have been designed and processed to fully express the gymnastic function of the body and recovery.

The present invention relates to a food additive composition comprising porridge salt as an active ingredient. More specifically, the composition may be a food substitute composition for salt replacement, including porridge salt as an active ingredient. The salt may be a refined salt. The salt substitute food additive composition may be a food additive composition having an improved or preventive effect on diabetes, that is, a significantly lower diabetes-inducing effect or a diabetic improvement effect.

The porridge salt refers to a salt of purple or bluish color among salts added with sun salt in bamboo. More specifically, the porridge salt is a process of filling and sealing the sun salt in bamboo; Performing the process of heating the sealed natural salt at 500 to 850 ° C., preferably at 600 to 800 ° C. for 1 hour to 10 hours, preferably 3 to 8 hours, and grinding the heated natural salt to eight times. After filling and sealing the natural salt in which the first firing step and the first firing step were carried out in bamboo, and then 1 to 10 hours, preferably 2 to 5 hours at 1000 to 2000 ℃, preferably 1300 to 1800 ℃ It may be prepared by a method comprising a second firing step of heating.

Filling and sealing the natural salt of the first firing step in the bamboo is a method of mixing by mixing the natural salt and bamboo in a 1: 1 to 1:10, preferably 1: 6 to 1: 9 based on the weight ratio Can be done.

The sun salt may be manufactured in Korea, and specifically, may be produced in the Jeollanam-do region.

The sun salt is preferably salt, that is, the sodium chloride content of 75 to 95% by weight, the calcium content of 0.1 to 0.3% by weight, the potassium content of 0.15 to 0.45% by weight, and the magnesium content of 0.4 To 2.0% by weight, preferably sodium chloride content of 80 to 90% by weight, calcium content of 0.11 to 0.22% by weight, potassium content of 0.2 to 0.35% by weight, magnesium content of 0.6 to 1.5% by weight More preferably, sodium chloride content of 82.5 to 88% by weight, calcium content of 0.13 to 0.18% by weight, potassium content of 0.25 to 0.33% by weight, magnesium content of 0.7 to 1.2% by weight, and In addition, it may further include manganese (Mn), iron (Fe), copper (Cu), zinc (Zn) and strontium (Sr).

The sodium chloride content may be 82.5 to 88% by weight, preferably 83 to 87% by weight, more preferably 84 to 86% by weight, the calcium content may be 0.13 to 0.18% by weight, preferably 0.14 to 0.16% by weight The potassium content may be 0.25 to 0.33% by weight, preferably 0.27 to 0.32% by weight, more preferably 0.29 to 0.31% by weight, and the magnesium content is 0.7 to 1.2% by weight, preferably 0.8 to 1.1% by weight, more Preferably from 0.9 to 1.0% by weight.

The bamboo is a generic name of an evergreen perennial plant belonging to a monocotyledonous plant rice plant and bamboo subfamily, preferably, the maturity of bamboo may be three to four years old. The bamboo is less than one week after cutting sap, so that the sap is used immediately after cutting, specifically those that have elapsed less than six days have elapsed, more specifically within one hour to 144 hours after cutting Do. As an example, the bamboo may be to use the form of chips (chip) after crushing the bamboo maturity of three to four years old. The size of the bamboo chip may be, for example, 1cm × 2cm in size in consideration of the degree of mixing with salt, productivity and process cost.

The second firing step is a process for heating for 30 minutes to 7 hours, preferably 1 to 4 hours at 1000 to 1300 ℃ and for 30 minutes to 3 hours, preferably 1 to 2 hours at 1500 to 1600 ℃ It may be made of a process.

In the heating process, the heating source may be a heat source such as pine wood fire, rosin, electricity or gas, and by heating at a constant temperature, it is possible to use electricity or gas to enhance the physiological activity of porridge.

The porridge salt prepared by the preparation method was found to have a remarkably excellent antidiabetic effect, that is, diabetes prevention or improvement effect, compared to the salt before processing. More specifically, the suicidal salt may impart salty taste to the food, but unlike salt, the effect of causing diabetes is remarkably reduced, and a diabetic improvement effect is recognized. Or may have an improvement effect.

Diabetes is an absolute or relative lack of insulin secretion in the interest, but due to a decrease in the biological effect of insulin on the cells, that is, a hyperglycemic state caused by insulin deficiency or decreased insulin sensitivity and associated metabolic disorders for a long time Refers to a disorder of glucose metabolism characterized by the condition. The above-mentioned diabetes is largely classified into type 1 diabetes and type 2 diabetes. The type 1 diabetes, namely insulin-dependent diabetes, is caused by decreased production and secretion of insulin due to selective damage to the pancreatic beta cells that secrete insulin. The type 2 diabetes, ie, insulin-independent diabetes, occurs when insulin resistance of the beta cells is not sufficiently exerted when the insulin resistance of peripheral tissues (liver, fat or liver) increases for any reason. do.

Salt replacement food additive composition containing the porridge salt as an active ingredient, glucose tolerance test, glucose uptake test in adipocytes and muscle, investigation of expression level of insulin-related protein in adipose tissue using animal experiment And as a result of blood lipid peroxide measurement, it is expected that the blood glucose lowering effect is recognized by reducing insulin resistance, increasing insulin sensitivity, and consequently lowering the blood glucose level, and thus, it is expected to have an excellent effect especially on type 2 diabetes.

The food additive composition comprising the suicide salt of the present invention as an active ingredient may include 0.001 to 99.99% by weight, preferably 0.1 to 99% by weight of the active ingredient, based on the total weight of the composition.

The food means a natural product or a processed product containing one or more nutrients, and preferably means a state in which it can be directly eaten through some processing process.

The food additive refers to a substance which is added or mixed with food for the purpose of manufacturing, processing or preserving food to improve the quality of the food, and used in food in addition to the raw materials or the main ingredient in the manufacturing, processing or storage of the food. It is a concept that includes the substance to be added and the additives prescribed in Article 2 (3) of the Food Sanitation Act.

Since the food additive may be used for the purpose of imparting saltiness to food, it may be used as a seasoning for replacing salt, soy sauce or miso in this sense, and to suppress, improve or prevent diabetes-induced effects such as salt intake. Since it is intended to be, it may preferably be a salt substitute food additive.

In this sense, the food additive may be a salt substitute food additive.

While the food additives impart salty taste, such as salt, antidiabetic effect is recognized for diabetes, in this respect the food additives may correspond to functional foods.

The food additive may further include food acceptable food additives, and may further include appropriate carriers, excipients and diluents commonly used in the preparation of food additives or functional foods. The carriers, excipients and diluents can be used independently or in combination. The proportion of auxiliary additives additionally included such carriers, excipients and diluents is not so critical, but may be selected from 0 to 20000 parts by weight per 100 parts by weight of the active ingredient of the present invention.

Although the composition of the present invention contains a large amount of sodium chloride, it has been confirmed that it can cause diabetes in the past, unlike purified salts mainly used for food, it is confirmed to reduce insulin resistance and increase insulin sensitivity, resulting in a blood glucose Since lowering the blood sugar lowering effect is recognized, it can be usefully used in patients with diabetic diseases or individuals at such risks, and in particular for the purpose of imparting a salty taste to the patient or the food ingested by the individual. I think there will be.

In addition, the present invention provides a method for producing porridge, which has an improvement or prevention effect on diabetes.

The manufacturing method is a process of filling and sealing the natural salt in bamboo; Performing the process of heating the sealed natural salt at 500 to 850 ° C., preferably at 600 to 800 ° C. for 1 hour to 10 hours, preferably 3 to 8 hours, and grinding the heated natural salt to eight times. After filling and sealing the natural salt in which the first firing step and the first firing step were carried out in bamboo, and then 1 to 10 hours, preferably 2 to 5 hours at 1000 to 2000 ℃, preferably 1300 to 1800 ℃ It may be a method including a second firing step of heating.

The first firing step is a process of filling and sealing salt into bamboo; The sealed salt may be heated by 600 to 800 ° C. for 3 hours to 8 hours and the grinding of the heated salt may be repeated seven times.

Filling and sealing the natural salt of the first firing step in the bamboo is a method of mixing by mixing the natural salt and bamboo in a 1: 1 to 1:10, preferably 1: 6 to 1: 9 based on the weight ratio Can be done.

The sun salt may be manufactured in Korea, and specifically, may be produced in the Jeollanam-do region.

The sun salt is preferably salt, that is, the sodium chloride content of 75 to 95% by weight, the calcium content of 0.1 to 0.3% by weight, the potassium content of 0.15 to 0.45% by weight, and the magnesium content of 0.4 To 2.0% by weight, preferably sodium chloride content of 80 to 90% by weight, calcium content of 0.11 to 0.22% by weight, potassium content of 0.2 to 0.35% by weight, magnesium content of 0.6 to 1.5% by weight More preferably, sodium chloride content of 82.5 to 88% by weight, calcium content of 0.13 to 0.18% by weight, potassium content of 0.25 to 0.33% by weight, magnesium content of 0.7 to 1.2% by weight, and In addition, it may further include manganese (Mn), iron (Fe), copper (Cu), zinc (Zn) and strontium (Sr).

The sodium chloride content may be 82.5 to 88% by weight, preferably 83 to 87% by weight, more preferably 84 to 86% by weight, the calcium content may be 0.13 to 0.18% by weight, preferably 0.14 to 0.16% by weight The potassium content may be 0.25 to 0.33% by weight, preferably 0.27 to 0.32% by weight, more preferably 0.29 to 0.31% by weight, and the magnesium content is 0.7 to 1.2% by weight, preferably 0.8 to 1.1% by weight, more Preferably from 0.9 to 1.0% by weight.

The bamboo is a generic name of an evergreen perennial plant belonging to a monocotyledonous plant rice plant and bamboo subfamily, preferably, the maturity of bamboo may be three to four years old. The bamboo is less than one week after cutting sap, so that the sap is used immediately after cutting, specifically those that have elapsed less than six days have elapsed, more specifically within one hour to 144 hours after cutting Do. As an example, the bamboo may be to use the form of chips (chip) after crushing the bamboo maturity of three to four years old. The size of the bamboo chip may be, for example, 1cm × 2cm in size in consideration of the degree of mixing with salt, productivity and process cost.

The second firing step is a process for heating for 30 minutes to 7 hours, preferably 1 to 4 hours at 1000 to 1300 ℃ and for 30 minutes to 3 hours, preferably 1 to 2 hours at 1500 to 1600 ℃ It may be made of a process.

In the heating process, the heating source may be a heat source such as pine wood fire, rosin, electricity or gas, and by heating at a constant temperature, it is possible to use electricity or gas to enhance the physiological activity of porridge.

The prepared porridge has a purple or blue color, stimulates appetite with a distinctive color and a unique aroma, and enhances aesthetics when used in food, as well as sensually, and has been previously identified as an edible food that can cause diabetes. Unlike purified salts and the like, which are mainly used, since the body can increase insulin functionality, lower insulin resistance, and lower blood sugar, there is an advantage that it can be usefully used in patients with diabetic diseases or individuals having such a risk.

As described above, the food additive composition comprising the sucrose salt of the present invention as an active ingredient not only maintains salty taste, but also has excellent palatability by the aroma and color characteristic of sucrose salt by bamboo and sun salt, By reducing insulin resistance and increasing insulin sensitivity, blood sugar lowering effect is recognized by lowering blood sugar level, so it can act effectively on type 2 diabetes, which has become a problem recently, and since it has been used for a long time as a food, side effects are not a problem. In addition, it can be usefully used for dietary control of diabetic patients or high-risk individuals for the disease. In addition, since the main raw materials are natural salts produced in abundantly on the west coast of Korea, especially Jeollanam-do and bamboo, which are native to Jeonnam, Korea, it is economically advantageous and the industrial effect will be very large.

1 is a graph of the glucose tolerance test of sun salt and anti-diabetic purpura according to an embodiment of the present invention, the horizontal axis means time, the vertical axis means blood sugar (mg / dL), SS Is a sun salt-administered group, PBS means a group treated with porphyritis.
Figure 2 is a graph confirming the glucose transport ability of the sun salt and the anti-diabetic suicidal infection according to an embodiment of the present invention, SS means sun salt administration group, PBS means suicide infection administration group, Adipose tissue in fat cells Skeletal muscle refers to the measurement result in muscle cells.
Figure 3 is a photograph confirming the effect on the protein associated with insulin sensitivity of the sun salt and anti-diabetic suicidal infection according to an embodiment of the present invention, SS means sun salt administration group, PBS means suicide infection administration group, pIRS1ser ³⁰⁷ And NF-κB each represent a band corresponding to a protein.
Figure 4 is a graph confirming the degree of protein expression associated with insulin sensitivity of the sun salt and anti-diabetic suicidal infection in accordance with an embodiment of the present invention, SS means sun salt administration group, PBS means suicide infection administration group, Figure 4a The expression level for pIRS1ser ³⁰⁷ is shown, and FIG. 4B shows the expression level for NF-κB.
5 is a graph confirming the effect on the production of lipid peroxides of sun salt and anti-diabetic suicidal salt according to an embodiment of the present invention, SS means sun salt administration group, PBS means suicide infection administration group.

Hereinafter, preferred embodiments of the present invention are described. However, the following examples are only preferred embodiments of the present invention, and the present invention is not limited to the following examples.

Example 1: Preparation of porridge

Solar salts used in the experiment (SS, solar salts) were purchased from Taepyeong salt (Korea). In addition, the porridge salt (PBS, purple bamboo salt) of the present invention was prepared by the same method as described above.

Specifically, after filling the three-year and four-year-old bamboo processed in the form of natural salt and chips in a 1: 7 (sun salt: bamboo) based on the weight ratio to the bamboo and sealed, the sealed salt temperature range of 600 to 800 ℃ After heating for 5 hours, the heated salt was ground. The pulverized salt was filled with the same method as that described above, i.e., the filling and heating was repeated seven times, and the same process was repeated a total of eight times to perform the first firing step. After filling and sealing the salt, which is repeated in the first firing step again to bamboo, and then heated for 2 hours in the range of 1000 to 1300 ℃, and then heated for 1 hour in the range of 1500 to 1600 ℃ to perform a second firing step The porridge salt of the present invention was prepared.

Example 2: Measurement of Antidiabetic Effect

Example 2-1 Breeding of Experimental Animals

Experimental animals were used to purchase Spraque-Dawley male rats of 6 weeks old from Damul Science Co., Ltd. (South Korea). The rats were transferred to the breeding room three days before the experiment and stabilized for two weeks. The rats were divided into eight groups, each divided into a group treated with porphyritis and a group containing sea salt. More specifically, the breeding was carried out in two cages per cage in the breeding room, and was bred under the same conditions except for the difference in feed related to the type of salt. In relation to the breeding conditions, the photoperiod condition of the experimental animal was adjusted every 12 hours day and night, the temperature condition was adjusted to 18 ℃ to 24 ℃, relative humidity conditions were adjusted to 50% to 60%.

In the case of the feed, each salt of the porridge salt group and sun salt group was prepared by adding to the general feed so that the salinity of the feed is 8% by weight (w / w). For each experimental animal, feed containing porridge salt (porcine salt group, PBS) and sun salt (sun salt group, SS) was ingested.

Example 2-2: Oral glucose tolerance test (OGTT)

In connection with the hypoglycemic effect of the experimental animals bred by the method of Example 2-1, glucose tolerance test was measured. More specifically, after four weeks of breeding the experimental animals in each of the diet, and fasted for 12 hours. After the fasting, 4 mg / g bodyweight of glucose was intraperitoneally administered, and blood was collected from the tail vein at intervals of 0, 30, 60, 120, 180 and 300 minutes to measure blood glucose. Blood glucose was measured using a blood glucose meter (Ji Doctor, Green Cross, Korea). The measurement results are shown in Fig.

As shown in FIG. 1, the experimental group ingested porridge salt was found to reduce insulin resistance compared to the experimental group ingested sun salt. More specifically, the highest blood sugar level was shown after 1 hour of glucose administration, with the difference of 159.3 mg / dL in the sun salt group and 135.9 mg / dL in the suicidal group, and continued to decrease after 1 hour of glucose administration. And the difference between the groups were very significant. It was determined that the high salt diet induced insulin resistance, which is a pre-diabetes stage of diabetes, and that insulin resistance decreased when ingested porridge compared to sun salt.

Example 2-3 Measurement of Glucose Uptake in Adipose Cells and Muscles

After 4 weeks, the animals were sacrificed, and the fat and muscle tissues collected from the animals were washed in Kreb-henseleit buffer (pH 7.4) and preincubated at 37 ° C. Then, 2-Deoxyglucose and insulin were added. It was incubated again. After incubation, the tissues were washed in physiological saline, homogenized with 0.1N HCl, hydrolyzed at 95 ° C, cooled and neutralized with 0.1N NaOH, and neutralized with 0.5M triethanolamide buffer (TEA, pH8.1, containing 0.1% BSA). Mix and centrifuge. After the glucose assay cocktail was added to the centrifuged supernatant and reacted at 37 ° C. for 90 minutes, excitation at 530 nm with fluorescence and emission at 590 nm were performed to measure 2-Deoxyglucose content extracted from fat and muscle tissue cells. At this time, 2-deoxyglucose was used as a standard solution, and the standard solution was reacted in the same manner, and a standard curve was used as a standard for calculating 2-deoxyglucose content in the tissue. The measurement results are shown in Fig.

As shown in FIG. 2, the glucose transport ability in the adipocytes was not significantly different between groups according to the presence or absence of insulin, but the glucose transport ability in the suicide group was higher than that in the sun salt group. As a result of glucose transport ability test in muscle cells, glucose transport ability according to the presence or absence of insulin was higher in the suicide group than the sun salt group. In other words, when insulin was added, glucose uptake of sucrose group was higher than that of sun salt group, and it was judged that ingestion of sucrose salt had an effect on improving insulin resistance. In addition, glucose-transporting ability of the suicide group was high in both adipocytes and muscle tissues not treated with insulin, which may be attributed to the fact that suicide treatment group has a good insulin action, that is, high insulin sensitivity.

Example 2-4: Western blotting

Insulin resistance is that the insulin signaling process is not normally performed, and the signaling system is disturbed by a certain factor, which can be confirmed by investigating protein expression of the signaling system after the insulin receptor. Insulin receptor substrate-1 (IRS-1) is a major protein for intracellular signaling of insulin. It is phosphorylated by TNF-α expressed by oxidative stress, which inhibits its activity and reduces PI3-kinase activity. In addition, the expression of NF-kB in the cells is also increased by TNF-α, thereby inhibiting the activity of PPARγ, thereby inhibiting the expression of GLUT4, resulting in elevated blood glucose levels. In this regard, insulin sensitivity or insulin resistance can be confirmed by investigating the expression levels of two proteins of serine (pIRS1Ser ³⁰⁷ ) and NF-kB, amino acid serine 307 of IRS-1. Therefore, to confirm the expression of the protein in muscle tissue and adipocytes, Western blotting was performed.

More specifically, muscle tissue was separated from the experimental animals sacrificed after 4 weeks. After homogenizing the muscle tissue with lysis buffer, centrifugation (15,000rpm, 4 ℃, 15min), the separated supernatant was subjected to protein quantification by Bradford assay. To investigate the expression level of NF-kB and p-IRS-1ser307, electrophoresis was performed on 10% SDS PAGE gel, and then transferred to PVDF (Polyvinylidene Fluoride) membrane, and the transferred membrane was treated in blocking buffer. Anti-NF-kB p65 (Polyclonal Antibody, Upstate, USA) and Anti-IRS-1, pre CT (Upstate, NY, USA) were used as primary antibodies, and goat anti rabbit IgG (H & L) (Millipore, USA) and developed the film by reacting with a chemiluminescent substrate. The measurement results are shown in FIGS. 3 and 4.

As shown in FIG. 3 and FIG. 4, the phosphorylation of IRS-1 was examined from muscle tissues of rats fed the natural salt and sucrose, respectively, and the concentration of the expression level in the sucrose group was lower. In order to clearly see the difference between these proteins, the expression level of porridge salt was quantified based on the expression level of the sun salt group by using an image analyzer and shown in FIG. 4. As a result, the expression level of pIRS1ser ³⁰⁷ (FIG. 4A) in the suicide group was about 0.5, which confirmed that ingestion of sucrose lowered insulin resistance. In addition, as a result of confirming the expression level of NF-κB in adipocytes, the sucrose group was expressed somewhat lower than the sun salt group. These results indicate that the intake of porphyritis lowers insulin resistance, and one of the causes is due to an improvement in insulin signaling than sun salt. Insulin signaling improvement was confirmed by an increase in glucose uptake upon insulin treatment.

Example  2-5: lipid peroxides in serum Thiobarbituric Acid Reactive Substance , TBARS ) Measure

Diabetes mellitus is mainly caused by oxidative stress. To determine the effect of intake of sucrose on oxidative stress, lipid peroxide production was examined. More specifically, after 4 weeks, the sacrificial animals were sacrificed and the serum separated from the blood collected from the experimental animals was mixed with 20% trichloroacetic acid (TCA) and 1 M thiobarbituric acid (TAB), and then 90 ~ It reacted at 95 degreeC. Thereafter, n-butanol was added to the cooled reaction solution, and n-butanol layer containing malondialdehyde (MDA) -TBA reactant was obtained by centrifugation. The absorbance at 532 nm was measured for the obtained n-butanol layer. At this time, the standard solution MDA was reacted in the same manner as the sample solution to prepare a standard curve to determine the TBARS content in the serum. The measurement results are shown in Fig.

As shown in FIG. 5, the content of lipid peroxide was slightly lower than that of the sun salt in the porridge. From the above results, it was expected that the intake of porridge salts may further reduce oxidative stress as compared to sun salts.

 In summary, in the case of porridge, anti-diabetic effect is recognized, and when a salt such as tablet salt currently used for food is replaced with a food additive composition containing the porridge as an active ingredient, It has been found that it can prevent or ameliorate diabetes rather than induce diabetes otherwise. In addition, since the natural salt and bamboo, which are the raw materials of the porridge, are obtained from natural products used as food materials, side effects and safety problems of existing diabetes treatments do not occur. In addition, the west coast of our country, in particular, Jeollanam-do corresponds to the world's five major salt salts to obtain the natural salt, Korea, in particular, Jeollanam-do area corresponds to the production of bamboo, all of the active ingredients of the present invention can be easily obtained in Korea It is economically advantageous.

Claims (3)

Food additive composition for replacing salt containing porridge salt as an active ingredient. The salt replacement food additive composition of claim 1, wherein the salt is a refined salt. The salt replacement food additive composition of claim 2, wherein the salt replacement food additive composition has an improvement or preventive effect on diabetes.

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