KR20120111319A - Pheromonal compounds for induction and control of scrobipalpa salinella and uses thereof - Google Patents

Abstract

PURPOSE: A pheromone composition for attracting and eliminating Scrobipalpa salinella is provided to early prevent damage by Scrobipalpa salinella and to be used as an eco-friendly agrochemical. CONSTITUTION: A composition for attracting or preventing Scrobipalpa salinella contains 1-20 parts by weight of (E)-3-dodecenylacetate or (Z)-5-dodecenyl acetate based on 100 parts by weight of (Z)-3-dedecenyl acetate. The compounds are derived from pheromone grand of female Scrobipalpa salinella. The composition is manufactured in the form of a solution, emulsion, suspension, paste, gel, cream, lotion, oil, powder, wax, or spray.

Description

Pheromonal compounds for induction and control of Scrobipalpa salinella and uses approximately}

The present invention relates to a horn horn moth attracting, sperm pheromone composition for control and its use using a sex pheromone component secreted by the horned moth female.

Salicornia in general herbacea (English: glasswort) is a perennial herb that grows on the tidal flats of the southwestern coast of Korea and is distributed in groups around tidal flats and salt fields close to seawater. Stalk is very salty and is the only plant on the planet that absorbs salt. It is rich in various minerals such as Na, Ca, Mg, K, and Fe, and its value is gradually increasing.

The horned horn moth is now causing great damage in the cultivated area. Scrobipalpa salinella , whose larvae hit spider webs on the stems of the stalks, eats the leafy flesh , and, in the case of severe damage, the stalks cannot be harvested at all.

Cultivated farmers and industries have been researching and making efforts to solve the damage caused by the horned moth, but it is difficult to control pests by using pesticides due to the characteristics of the cultivated eco-friendly cultivars, and no proper control methods have been developed so far. It is true.

The present inventors have studied the eco Salicornia horns moth control methods to prevent damage caused by the Salicornia (Scrobipalpa salinella), the result sought, Salicornia horns moth (Scrobipalpa Salinella ) was designed to control the sex pheromone, and for this purpose, we first identified the sex pheromone of females, and then found the most suitable composition for attracting adult males, and applied it as a result. Scrobipalpa salinella The present invention has been completed by discovering that it is possible to drastically reduce the damage caused by bumps.

Accordingly, an object of the present invention is to provide a composition for attracting or controlling a ragged moth moth using a sex pheromone-derived material of rhododendron moth.

It is another object of the present invention to provide a pheromone trap or mating disturbance agent comprising the composition for attracting or controlling the rhabdomite moth.

In order to solve the above problems,

The present invention comprises at least one compound selected from the group consisting of (Z) -3-dodecenyl acetate, (E) -3-dodecenyl acetate and (Z) -5-dodecenyl acetate. Provided is a horn moth attraction or control composition.

In another aspect, the present invention provides a pheromone trap or copious disturbance agent comprising the composition for attracting or controlling the rhododendron moth.

The horned horn moth attracting and controlling sex pheromone composition according to the present invention is the most suitable pheromone lure (bait) for attracting horned horned moth males, and can prevent the damage of the horned ear by the horned horn moth, It has the advantage that it can be used as an environmentally friendly agricultural material by producing it as a mass killing and mating disturbance agent. In addition, it is possible to reduce the foreign currency by localization of the preservatives, and contribute to the production of lumps in an eco-friendly way.

Figure 1 shows the results of GC-MS analysis of the female sex pheromone extract of the horned horn moth female.
Figure 2 is a graph showing the effect of Z3-12: OAc and Z5-12: OAc mixed composition pheromone (standard) and single-treated pheromone on the male attraction of male hornworm moth.
3 is a graph showing the attracting effect of male horned moth male according to the composition of pheromone in the open air (Tukey's test: P> 0.05).
Figure 4 is a photograph showing the male adult male snapper moth attracted to the pheromone trap.

In the present invention, the sex pheromone of the horned rhinoceros moth has not been identified for the first time, and the optimum composition ratio for the analysis and identification of the horned rhinoceros moth attracted to maximize the effect of attracting and controlling the horned rhinoceros moth It is a main feature to provide a composition which shows.

The present invention relates to a sex pheromone composition for environmentally friendly control of spurs (seaweed) from spurs, more specifically (Z) -3-dodecenyl acetate (Z3-12: OAc), (E)- 3-Dodecenyl Acetate (E3-12: OAc) and (Z) -5-Dodecenyl Acetate (Z5-12: OAc) composition comprising one or more compounds selected from the group consisting of horn horn moth attracting or controlling And it relates to a mating disturbance agent or pheromone trap using the same.

Hereinafter, these are demonstrated concretely.

At least one member selected from the group consisting of (Z) -3-dodecenyl acetate, (E) -3-dodecenyl acetate and (Z) -5-dodecenyl acetate Compound.

More preferably (Z) -3-dodecenyl acetate, and (E) -3-dodecenyl acetate and (Z) -5-dodecenyl acetate. It is preferable because it shows a better effect on attracting or controlling the ragged moth.

The use ratio between the compositions is not limited, but is preferably selected from (E) -3-dodecenyl acetate and (Z) -5-dodecenyl acetate with respect to 100 parts by weight of (Z) -3-dodecenyl acetate. It is preferable to include 1 to 20 parts by weight of one or more compounds, and more preferably, 5 to 15 parts by weight is preferable because it shows the best effect on attracting moth.

(Z) -3-dodecenyl acetate (Z3-12: OAc), (E) -3-dodecenyl acetate (E3-12: OAc) and (Z) -5-dodecenyl acetate (Z5-12: OAc The compound can be separated from the female sex pheromone gland, and, for example, it can be separated by extracting the sex pheromone gland located in the female abdomen in an organic solvent.

Compositions for attracting or controlling the rhabdomyomoth moth of the present invention comprising the compound may be prepared in various forms using methods known in the art, for example, (Z) -3-dodecenyl acetate (Z3). -12: OAc) and (E) -3-dodecenyl acetate (E3-12: OAc) are mixed in an organic solvent and deposited on a carrier such as a rubber septa to volatilize the organic solvent and only the pheromone component to the carrier. It can be made to soak.

According to the present invention, the horned moth attracting or controlling composition can be prepared in various formulations and used as an attractant or controlling agent of the horned moth. The form of the formulation of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, emulsions, suspensions, emulsions, pastes, gels, creams, oils, powders, waxes and sprays It may be formulated as a formulation, but is not limited thereto.

When the formulation of the present invention is a cream, as an additive, a liquid diluent such as ethyl alcohol, water or propylene glycol, lanolin oil, acetylated lanolin alcohol, cetyl alcohol, stearyl alcohol, biswax, BHT, alkyl Parabens and the like can be used. When the formulation of the present invention is in gel form, alkylene glycol, water, carbomer 940 and the like may be used as additives. When the formulation of the present invention is a spray, a liquid diluent such as water, ethyl alcohol or propylene glycol may be used as an additive.

The horn bitter moth attracting or controlling composition according to the present invention can be utilized as a pest antagonist that is pest pest observation and environmentally friendly control agent, for example, a copulation disturbance agent comprising the horn moth attracting or controlling composition according to the present invention. Alternatively, the pheromone trap may be manufactured and used.

More specifically, the horn horn moth attracting or controlling composition according to the present invention is used by installing around the horn, and can be used as a mating disturbance agent to disturb the copulation of the horn horn moth. For example, the horn horn moth attracting or controlling composition of the present invention may be prepared in the form of a cream and attached to a desired portion of the horn. However, the method of using the pheromone composition of the present invention is not limited thereto, and the method of use may vary depending on the surrounding environment. . In addition, more preferably, a food trap may be disposed around the crop to prevent movement of the pest male from the untreated area and used with the pheromone composition. In addition, when used, it is possible to mix and use an electrodeposition agent. The control method using the pheromone composition of the present invention can be applied to all crops that can be controlled by pests disturbed by mating disturbance, in particular can be applied to all salt plants to which the rhizome moth is added.

In the case of the pheromone trap according to the invention, for example, a mixture of (Z) -3-dodecenyl acetate (Z3-12: OAc) and (E) -3-dodecenyl acetate (E3-12: OAc) was deposited. The rubber diaphragm can be used outdoors as a lure of a pest attraction trap with a sticky plate inserted to effectively protect it from the horn moth.

Hereinafter, the present invention will be described in detail with reference to Examples and Test Examples. However, these are for the purpose of illustrating the present invention in more detail, and the scope of the present invention is not limited thereto.

Example  One: Horned Horn Moth  Extraction and Analysis of Pheromone Components in Females

At night, when mating horned moths are mainly mated, females that have not yet been mated were captured, and the abdominal end where the pheromone gland was located was cut with scissors and put in an organic solvent (hexane) to obtain an extract. The obtained extract was analyzed by GC-MS equipped with a DB-23 column. As a result, the sex pheromone secreted by the female horned moth was (Z) -3-dodecenyl acetate (Z3-12: OAc), (E ) -3-dodecenyl acetate (E3-12: OAc) and (Z) -5-dodecenyl acetate (Z5-12: OAc), among which (Z) -3-dodecenyl acetate (Z3-12) : OAc) was the main component.

The results are shown in Table 1 and FIG. 1.

Amount and Ratio of Sex Pheromone Components Secreted by Female Horned Horn Moths Sex Pheromone Ingredients Extraction amount (ng / female) ratio(%) Z 3-12: OAc 2.15 91 E 3-12: OAc 0.14 6 Z 5-12: OAc 0.07 3

As can be seen from the above results, the sex pheromone of the female of the horned moth female is (Z) -3-dodecenyl acetate (Z3-12: OAc), (E) -3-dodecenyl acetate (E3-12) : OAc) and (Z) -5-dodecenyl acetate (Z5-12: OAc), and the ratio was about 91: 6: 3.

Example  2: outdoor pheromone alone and mixed use Horned Horn Moth  Male Attraction Effect Test

The mixed composition of (Z) -3-dodecenyl acetate (Z3-12: OAc) and (Z) -5-dodecenyl acetate (Z5-12: OAc) and pheromone alone In order to investigate the influence on the male horn moth male attraction, the male horn moth male attracting effect was tested using the pheromone composition shown in Table 2 below.

Measurement results for the male horned moth male moth effect according to the composition of pheromone in the open air are shown in Table 2 and FIG. 2. The measurement was carried out by installing lures with different pheromone compositions in outdoor pavement where the stalks were grown, and examining the number of males attracted after one week.

Furtherance Z3-12: OAc + Z5-12: OAc
(standard) Z3-12: OAc E3-12: OAc Z5-12: OAc E5-12: OAc Control usage
(unit) 0.9 +0.1 mg 1.0 mg 1.0 mg 1.0 mg 1.0 mg 0 mg Number of males attracted per trap 58.6 2.8 0.4 1.0 1.8 0.8

As shown in the above results, the mixed pheromone component of (Z) -3-dodecenyl acetate (Z3-12: OAc) and (Z) -5-dodecenyl acetate (Z5-12: OAc) was found It showed a high attraction effect, and the single-treated pheromone component was found to have almost no attraction effect.

Example  3: Outdoor composition of pheromone Horned Horn Moth  Male Attract Effect

In order to learn more about the male horned moth male attracting effect according to the pheromone mixed composition in the open air, the male horned male moth attracting effect was confirmed by the pheromone composition shown in Table 3 below in the outdoor packaging in which the horned cultivated. The measurement results are shown in Table 3 below. The measurement was carried out by installing lures with different pheromone compositions in outdoor pavement where the stalks were grown, and examining the number of males attracted after one week.

division A B C D E F G Control Z3-12: OAc 0.9 mg 0.5 mg 0.1 mg 0.9 mg 0.5 mg 0.1 mg 0.8 mg - E3-12: OAc 0.1 mg 0.5 mg 0.9 mg - - - 0.1 mg - Z5-12: OAc - - - 0.1 mg 0.5 mg 0.9 mg 0.1 mg - Number of males attracted per trap 109.8 51.2 23.2 116.2 54.9 15.2 11.03 0.6

As can be seen from the above results, (E) -3-dodecenyl acetate (E3-12: OAc) or (Z)-to (Z) -3-dodecenyl acetate (Z3-12: OAc) which is a main component in the open air. When 5-dodecenyl acetate (Z5-12: OAc) was added, it was confirmed that the attracting effect of the male Rhododendron male was greatly improved. In addition, to attracting male horned moth males, (Z) -3-dodecenyl acetate (Z3-12: OAc) in 90 parts by weight of (E) -3-dodecenyl acetate (E3-12: OAc) or (Z)- It was confirmed that the mixture to which 10 parts by weight of 5-dodecenyl acetate (Z5-12: OAc) was added was suitable.

Manufacturing example  One: Horned Horn Moth  Preparation of Pheromone Trap Using Attract or Control Composition

(Z) -3-dodecenyl acetate (Z3-12: OAc) and (E) -3-dodecenyl acetate (E3-12: OAc) Or (Z) -3-dodecenyl acetate (Z3-12: OAc) and (Z) -5-dodecenyl acetate (Z5-12: OAc) in a ratio of 9: 1. The mixture was immersed in a rubber diaphragm (white, 8 mm in diameter, Aldrich) to prepare a pheromone luer.

Manufacturing example  2: Horned Horn Moth  Preparation of Mating Disorders Using Attractant or Control Composition

(Z) -3-dodecenyl acetate (Z3-12: OAc) and (E) -3-dodecenyl acetate (E3-12: OAc) or (Z) -3-dodecenyl acetate (Z3-12: OAc) And (Z) -5-dodecenyl acetate (Z5-12: OAc) were mixed in a ratio of 9: 1, respectively, to prepare a gel mating disturbance agent.

Manufacturing example  3: Gel  Produce

90 g of (Z) -3-dodecenyl acetate and 10 g of (E) -3-dodecenyl acetate or 90 g of (Z) -3-dodecenyl acetate and 10 g of (Z) -5-dodecenyl acetate were used. 30g was added and mixed with 30g of propylene glycol. 2 g of Carbomer 940 was added thereto to completely dissolve it, and then purified water was added thereto and mixed.

Manufacturing example  4: spray Spray Manufacturing

90 g of (Z) -3-dodecenyl acetate and 10 g of (E) -3-dodecenyl acetate or 90 g of (Z) -3-dodecenyl acetate and 10 g of (Z) -5-dodecenyl acetate were used here. 30 g glycol (Propylene glycol) and 10 g of ethyl alcohol (Ethyl alcohol) was added to the mixture and mixed with purified water.

Manufacturing example  5: cream Cream Manufacturing

90 g of (Z) -3-dodecenyl acetate and 10 g of (E) -3-dodecenyl acetate or 90 g of (Z) -3-dodecenyl acetate and 10 g of (Z) -5-dodecenyl acetate were placed in a container. A] was prepared. This was mixed with the following [Composition B]. Thereafter, a mixture of [Composition C] and [Composition D] was added thereto and mixed with a high speed mixer.

[Production of Composition A]

1. Composition of the present invention ..................... 100g

2.Propylene glycol ...... 30g

3. Lanolin alcohol ..... 4g

<Composition A> was prepared by mixing the materials of 1 to 3.

[Production of Composition B]

1.Cetyl alcohol ..................... 1.0g

2.Solulan C-24 ......... 0.3g

3. Stearic acid ..................... 3.0g

4. Glyceryl stearate .... 2.0g

The 1-4 materials were mixed and then melted by heating to 80 ° C. Then it was cooled to 30 ℃ to prepare <Composition B>.

[Production of Composition C]

Methyl gluceth-10 ......... 5.0g

2. Water (H ₂ O) ..................... 20.0 g

<Composition C> was prepared by mixing the materials of 1-2.

[Production of Composition D]

Methylparaben ......... 0.3g

2. Propylparaben ................ 0.25g

3. Water (H ₂ O) ...................... 10g

The 1-3 materials were mixed and then melted by heating to 80 ° C. Thereafter, it was cooled to 30 ° C to prepare <Composition D>.

Claims (7)

(Z) -3-dodecenyl acetate, (E) -3-dodecenyl acetate, and (Z) -5-dodecenyl acetate, at least one compound selected from the group consisting of Or a composition for control.
The method of claim 1,
(Z) -3-dodecenyl acetate; And
At least one compound selected from (E) -3-dodecenyl acetate and (Z) -5-dodecenyl acetate
Tongalung moth attracting or controlling composition comprising a.
The method according to claim 2, 1 to 20 parts by weight of at least one compound selected from (E) -3-dodecenyl acetate and (Z) -5-dodecenyl acetate relative to 100 parts by weight of (Z) -3-dodecenyl acetate. Composition for attracting or controlling the ragged horn moth, characterized in that it comprises.
The method according to claim 1, wherein the (Z) -3-dodecenyl acetate, (E) -3-dodecenyl acetate and (Z) -5-dodecenyl acetate are derived from sex pheromones of females Compositions for attracting or controlling the ragged horn moth.
The composition of claim 1, wherein the composition is formulated in the form of a solution, emulsion, suspension, emulsion, paste, gel, cream, lotion, oil, powder, wax or spray. Composition for attracting or controlling the rhabdomyomoth moth, characterized in that.
Pheromone trap, characterized in that it comprises a composition for attracting or controlling the ragged horn moth moth of any one selected from the group consisting of claims 1 to 4.
A mating disturbance agent comprising a composition for attracting or controlling the rhododendron moth in any one selected from the group consisting of claims 1 to 4.

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