KR20120087652A - Manufacturing Mathod of Ginger Extract And Ginger Extract Using The Same - Google Patents

Abstract

PURPOSE: A ginger extract and a producing method thereof are provided to use a supercritical extraction method for improving an anti-inflammation effect of the extract. CONSTITUTION: A producing method of ginger extract comprises the following steps: preparing ginger powder; extracting ginger powder using supercritical carbon dioxide at 35-55 deg C for 1-3 hours in the pressure of 100-400bar and the flow speed of 1-3mL per minute, using a supercritical extraction method to obtain ginger oil; and adding 60-90% of alcohol into residues obtained from extracting the ginger oil, settling the mixture for 6-36 hours, and filtering and concentrating the mixture. The average granularity of the ginger powder is 50-500 micrometers. The ginger extract contains 21-25wt% of gingerol.

Description

Manufacturing method of ginger extract and ginger extract according to it {Manufacturing Mathod of Ginger Extract And Ginger Extract Using The Same}

The present invention relates to a method for producing a ginger extract with high anti-inflammatory effect by using a supercritical extraction method and to a ginger extract accordingly.

Ginger is one of spices widely used not only in Korea but also in the world and is widely used as one of the seasoning ingredients added to improve the sensory properties of food. In addition, ginger contains a variety of ingredients beneficial to the human body has been widely used as tea (tea) or medicine.

Ginger is a subtropical and tropical perennial plant belonging to the family Ginger, and has been used as a spice for a long time due to its characteristic aroma and spicy taste. In oriental medicine, ginger root stems are used as a health medicine. Health is effective in indigestion and vomiting diarrhea, promotes blood circulation, anti-inflammatory and analgesic effect.

About three quarters of ginger is water, and about 40-60% of the total solids are made up of starch. Essential oils include zingiberol and zingiberene, and specific ingredients include 6-gingerol (0.1-0.3%), 8-gingerol and 10-gingerol. , Zingerone, 6-shogaol (0.04%), gamma-aminobutyric acid, and the like.

Extraction of natural products by supercritical fluids has been of great interest. Extraction and separation techniques using supercritical fluids are one of the separation techniques for selectively extracting specific components from the mixed components using specific physical properties of the supercritical fluid in or near the critical point.

Methods for separating specific components from the mixture include distillation using the difference in volatility of the components and solvent extraction using the difference in solubility in a specific solvent. Distillation is operated at a high boiling point, so in the distillation of natural products, decomposition and destruction of the active ingredient due to high temperature becomes a problem. Solvent extraction involves difficulties in selecting an appropriate organic solvent, remaining organic solvent in the extraction phase, the need for a removal process, and low separation effect. However, the supercritical fluid extraction method is a low energy consumption process because it can be operated at a low temperature near the critical temperature compared to the distillation method and is suitable for heat sensitive materials such as natural products. Compared with the solvent extraction method, the supercritical fluid has a high diffusion coefficient and low viscosity, so the extraction speed is fast, and phase separation is easy. Therefore, the residual solvent removal process is not required like the solvent extraction method.

Ginger shows high anti-inflammatory activity among various functionalities, but when it is developed various functional foods or pharmaceutical compositions for treating inflammation, it has a taste that is not satisfactorily good and peculiar aroma characteristic is followed.

Therefore, it is necessary to develop an extraction method that can not only remove or supplement the unique flavor and aroma of ginger but also increase the extraction yield in a short time, but also obtain an extract with a high content of gingerol, one of the anti-inflammatory active ingredients. In addition, it is necessary to develop not only ginger extract but also how to use ginger foil after supercritical extraction of ginger.

The present invention is characterized by obtaining ginger oil by supercritical extraction of ginger powder for 1 to 3 hours at a flow rate of 1 to 3 mL / min at a temperature of 35 to 55 ° C. and a pressure of 100 to 400 bar using supercritical carbon dioxide. It provides a method for producing ginger extract.

The present invention is also characterized in that the ginger remaining after obtaining the ginger oil is added to 60 ~ 90% alcohol of 5 to 15 times (w / v) with respect to the weight of ginger foil and left for 6 to 36 hours at room temperature It provides a method for producing an extract.

The present invention provides a ginger extract manufacturing method that can not only remove or supplement the unique flavor and aroma of ginger but also obtain a high extracting yield in a short time, but also a high content of gingerol (gingerol).

The present invention provides a ginger extract with high anti-inflammatory activity by utilizing the remaining ginger foil after supercritical extraction of ginger.

Accordingly, the present invention provides a step of preparing a ginger powder (S1) as a first embodiment; And (S2) ginger powder is extracted by supercritical extraction for 1 to 3 hours at a flow rate of 1 to 3 mL / min at a temperature of 35 to 55 ° C. and a pressure of 100 to 400 bar using supercritical carbon dioxide. It provides a method for producing ginger extract comprising;

In the ginger supercritical extract manufacturing method according to the embodiment, the ginger powder in the step (S1) provides a ginger extract manufacturing method having an average particle size of 50 ~ 500 μm.

In the ginger supercritical extract manufacturing method according to the embodiment, the extraction yield provides a ginger extract manufacturing method of 2.5 to 3.0% by weight.

In the ginger supercritical extract manufacturing method according to the above embodiment, after the step (S2) (S3) after the ginger oil obtained from the remaining 5 to 15 times (w / v) of 60 ~ 90% alcohol by the weight of ginger foil Adding and standing at room temperature for 6 to 36 hours; And (S4) filtration and concentration; provides a ginger extract manufacturing method further comprising.

The present invention provides a ginger extract prepared according to the above production method as a second preferred embodiment.

In the ginger supercritical extract according to the second embodiment, a ginger extract having a content of gingerol (gingerol) is 21 to 25% by weight.

In the ginger extract according to the second embodiment, it provides a ginger extract 50 to 98% of NO (Nitric Oxide) production inhibitory effect at a concentration of 10 ~ 100㎍ / mL.

The present invention provides an anti-inflammatory composition comprising the ginger extract as a third embodiment.

1 is a graph showing a change in yield of each supercritical extraction temperature and pressure of ginger according to an embodiment of the present invention.
Figure 2 is a photograph showing the appearance of the ginger extract extracted by varying the pressure at a temperature of 35 ℃ according to an embodiment of the present invention.
Figure 3 is a graph showing the antioxidant activity of the ginger extract extracted by varying the supercritical extraction temperature and pressure according to an embodiment of the present invention. At this time, Figure 3a shows the antioxidant activity of the supercritical extract ginger extract at 35 ℃, Figure 3b is 45 ℃, Figure 3c is a graph showing the antioxidant activity of the supercritical extract ginger extract at 55 ℃.
Figure 4 is a graph showing the cytotoxicity and NO scavenging ability of the ginger supercritical extract according to an embodiment of the present invention. Specifically, Figure 4a is a graph showing the cytotoxicity of the ginger supercritical extract, Figure 4b is a graph showing the NO scavenging ability of the ginger supercritical extract.
Figure 5 is a graph showing the cytotoxicity and NO scavenging ability of ginger supercritical extract according to an embodiment of the present invention. Specifically, Figure 5a is a graph showing the cytotoxicity of ginger supercritical gourd extract, Figure 5b is a graph showing the NO scavenging ability of the ginger supercritical gourd extract.
6 is a graph showing the cytotoxicity and NO scavenging ability of ginger ethanol extract according to a preferred comparative example of the present invention. Specifically, Figure 6a is a graph showing the cytotoxicity of ginger ethanol extract, Figure 6b is a graph showing the NO scavenging ability of the ginger ethanol extract.
7 is a graph showing the cytotoxicity and NO scavenging ability of gingerol. Specifically, Figure 7a is a graph showing the cytotoxicity of the ginger roll, Figure 7b is a graph showing the NO scavenging ability of the ginger roll.

Hereinafter, the present invention will be described in more detail.

The present invention provides a method for producing a ginger extract having a high anti-inflammatory effect by using a supercritical extraction method and a ginger extract accordingly, in particular ginger powder using a supercritical carbon dioxide temperature of 35 ~ 55 ℃, 100 ~ 400 Supercritical extraction for 1 to 3 hours at a flow rate of 1 to 3 mL / min at bar pressure not only eliminates or complements the ginger's distinctive taste and aroma, but also gives a high extraction yield in a short time, while gingering It provides a method of obtaining an extract having a high content of. If the respective conditions are out of range, the extraction yield is lowered or the extraction yield is no longer increased, which is economically undesirable.

In addition, the present invention is added to 60 ~ 90% alcohol of 5 to 15 times (w / v) with respect to the weight of the ginger foil remaining after the supercritical extraction and allowed to stand for 6 to 36 hours at room temperature, by using a ginger foil Provided is a method for obtaining ginger extract with high anti-inflammatory activity.

To this end, the present invention comprises the steps of preparing a ginger powder (S1); And (S2) ginger powder is extracted by supercritical extraction for 1 to 3 hours at a flow rate of 1 to 3 mL / min at a temperature of 35 to 55 ° C. and a pressure of 100 to 400 bar using supercritical carbon dioxide. It provides a method for producing ginger extract comprising;

First, the ginger powder is prepared in step (S1). Ginger powder has an average particle size 50 to 500 μm is preferable because the extraction rate is high.

In step (S2), the ginger powder is extracted by supercritical extraction for 1 to 3 hours at a flow rate of 1 to 3 mL / min at a temperature of 35 to 55 ° C. and a pressure of 100 to 400 bar using supercritical carbon dioxide.

According to the extraction method ginger oil (ginger supercritical extract) can be obtained in a yield of 2.5 to 3.0% by weight in a short time.

In addition, according to the extraction method, the ginger extract having a content of 21 to 25% by weight of gingerol, which is one of the anti-inflammatory active ingredients, can be obtained. That is, when the supercritical extraction of ginger powder under the above conditions, it is possible not only to remove or supplement the flavor and flavor peculiar to ginger, but also to extract extracts (ginger oil) having a high content of gingerol in a short time. You can get it.

In addition, the ginger extract manufacturing method of the present invention after the (S2) step, (S3) 60 ~ 90% alcohol of 5 to 15 times (w / v) with respect to the weight of the ginger foil remaining after obtaining ginger oil Adding and standing at room temperature for 6 to 36 hours; And (S4) filtering and concentrating.

In addition, according to the above method, a ginger extract having a supercritical extraction of ginger and 50 to 98% of NO (Nitric Oxide) production inhibitory effect at a concentration of 10 to 100 µg / mL can be obtained by using ginger foil. That is, according to the extraction method, the ginger extract having high anti-inflammatory activity can be obtained by using the remaining ginger foil after the supercritical extraction of ginger.

When the inflammatory reaction occurs, several proinflammatory mediators are produced, which are prostaglandin produced by nitric oxide (NO) and cyclooxygenase-2 (COX-2), which are produced by inducible nitric oxide synthase (iNOS). E ₂ (PGE ₂ ), and the like. These inflammatory factors activate nuclear factor-kB (NF-kB), a transcription factor of the inflammatory response, resulting in the production of excess NO and PGE ₂ to cause inflammation. Therefore, by inhibiting the production of nitric oxide (NO) it is possible to suppress the inflammatory response.

Next, the present invention provides an anti-inflammatory composition comprising a ginger extract according to the ginger extract manufacturing method.

The anti-inflammatory composition is a very useful invention because it can be used in various industrial fields, such as cosmetic compositions, pharmaceutical compositions, food compositions with anti-inflammatory functionality.

Hereinafter, the configuration of the present invention in more detail through examples, but the scope of the present invention is not limited to the following examples.

[ Example ]

Example  One

A supercritical fluid extractor (SFT-100XW, Supercritical Fluid Technologies, Inc., Newark, DE, USA) was used, and 18 g of ginger powder having an average particle size of 50 to 500 μm was extracted into a 100 mL extraction tank. The extraction conditions of the samples were carried out with varying pressure at 35, 45, 55 ℃ temperature to 100, 200, 300, 400 bar, the flow rate of carbon dioxide is 2 mL / min for 2 hours in the dynamic mode to extract the container immediately I received it in (vial).

Example  2

In Example 1, a ginger supercritical extract (ginger oil) was obtained, and 10 times (w / v) of 70% alcohol was added to the weight of the remaining by-product ginger foil, and allowed to stand at room temperature for 24 hours to obtain a ginger (bak) extract. .

Comparative example

10 times (w / v) of 70% spirit (ethanol) was added to the ginger powder having an average particle size of 50 to 500 μm, and left at room temperature for 24 hours to obtain a ginger extract.

[ Experimental Example ]

Experimental Example  1: ginger Supercritical  Extraction yield

The supercritical extraction yield of ginger increased with increasing pressure at the same temperature as shown in FIG. 1, while the yield decreased from 1.91% to 1.07% at 100 bar as the temperature increased from 35 ° C to 55 ° C. At 200-400 bar, the extraction yield increased with increasing temperature, showing 2.41, 2.92 and 3.00% at 55 ℃, respectively.

Experimental Example  2: Color and Appearance of Ginger Extract

The chromaticity was measured by using a color and color difference meter (Color QUESTⅡ, Hunter Lab, USA) to measure L (lightness), a (redness / greenness), and b (yellowness / blueness), with standard white plate L = 92.68 , a = -0.81 and b = 0.86 were used.

Figure 2 shows the appearance of the ginger extract extracted by varying the pressure at 35 ℃, it was yellow at 100 bar and as the pressure is increased a lot of ginger's own pigment components are extracted and the color becomes darker at 400 bar of the red system It is reddish brown. In addition, as a result of measuring the chromaticity of the ginger extract according to the supercritical extraction condition, as shown in Table 1, the L value and the b value decreased significantly with increasing pressure at all temperature conditions. It was found that the correlation between the pigment extraction and the b value was the largest.

Color of Ginger Extract by Supercritical Extraction Temperature and Pressure Temperature (℃) Pressure (bar) Color value L a b 35 100 38.8 ± 1.5 6.6 ± 1.0 26.7 ± 2.3 200 29.4 ± 0.6 10.0 ± 1.7 10.2 ± 1.9 300 28.6 ± 0.9 16.7 ± 2.4 9.2 ± 1.9 400 24.4 ± 1.4 5.0 ± 1.1 0.2 ± 0.9 45 100 44.4 ± 0.9 -0.9 ± 0.3 33.0 ± 1.8 200 34.0 ± 1.3 14.4 ± 1.0 18.7 ± 2.6 300 27.7 ± 0.8 14.7 ± 0.7 8.5 ± 1.1 400 24.7 ± 0.5 6.9 ± 0.7 1.2 ± 0.7 55 100 45.3 ± 3.0 -2.0 ± 0.3 30.9 ± 4.6 200 35.7 ± 1.2 12.6 ± 0.7 21.8 ± 1.8 300 24.1 ± 0.7 7.9 ± 1.2 2.8 ± 1.0 400 21.8 ± 0.7 5.0 ± 0.2 0.6 ± 0.1

Experimental Example  3: of ginger extract Gingerbread ( gingerol ) content

Gingerol, such as 6-gingerol, 8-gingerol, 10-gingerol, 6-shogaol, etc. contained in ginger supercritical extract Was determined using HPLC (Jasco Co., Japan). The column used a Waters symmetry C-8 reversed phase column (150 × 3.9 mm, Cat.No. WATO 54235), and the mobile phase eluted methanol-water (46:35, v / v) at a rate of 1 mL / min. The detection of the sample was measured at 282 nm with a UV detecter. Analytical standards 6- ginger roll, 8- ginger roll, 10- ginger roll, 6- shogaol was purchased from Chromadex. The ginger extract of Example 1 was dissolved in methanol at a concentration of 5 mg / mL and filtered by 0.45 μm syringe filter (Millipore), which was used as a sample for analysis.

The results of measuring the ginger roll content change of ginger extract according to supercritical extraction temperature and pressure are shown in Table 2 below. Ginger roll content distribution showed the highest content of 6- ginger roll (10.78 ~ 17.17%), 8- ginger roll (2.38 ~ 4.08%), 10- ginger roll (1.01 ~ 3.07%), 6- shogaol (0.64 ~ 1.09% ). Regardless of the extraction temperature, the lowest ginger roll content was shown at 100 bar, and as the extraction temperature increased, it decreased to 20.00, 18.02 and 15.13%, respectively. On the other hand, in the range of 200-400 bar, 21.46 ~ 24.48% showed no significant difference in gingerol content.

Changes in Ginger Roll Contents of Ginger Extracts by Supercritical Extraction Temperature and Pressure Temperature (℃) pressure
(bar) Gingerol content (%) 6-Ginger Roll 6-shogaol 8-Ginger Roll 10-ginger roll Sum 35 100 15.01 0.71 2.47 1.81 20.00 200 16.02 1.05 3.67 2.75 23.49 300 17.17 0.98 4.08 2.25 24.48 400 16.43 1.09 3.40 2.57 23.49 45 100 12.35 0.88 3.01 1.78 18.02 200 16.01 0.75 3.19 2.52 22.47 300 15.33 0.64 3.02 2.47 21.46 400 15.37 0.66 3.17 2.69 21.89 55 100 10.78 0.96 2.38 1.01 15.13 200 16.37 0.91 3.62 2.90 23.80 300 16.21 0.68 3.08 2.54 22.51 400 15.69 0.76 3.58 3.07 23.10

Experimental Example  4: Antioxidant Activity of Ginger Extract

DPPH (2,2- 피덴 -One- picryl - hydrazyl ) Radical Scatters

The antioxidant activity of the extract was measured by DPPH radical scavenging activity modified by the method of William et al. 1 mL of 0.15 mM DPPH solution dissolved in methanol and 0.05 mL of the diluted sample at each concentration were mixed, and after 20 minutes, the absorbance was measured at 525 nm. The radical scavenging activity of DPPH was expressed as a percentage by the following equation compared to the control without the sample.

(B: Absorbance of control / A: Absorbance of sample)

In addition, the radical scavenging activity of each extract was expressed as an IC ₅₀ value, which is the concentration of the extract necessary to reduce the absorbance of the control group without the extract to 1/2.

ABTS Radical Scatters

ABTS radical scavenging ability of the extract was measured by a modification of Van der Berg et al. The 7 mM ABTS solution prepared 12 to 16 hours before the experiment was diluted in 5 mM PBS to have an absorbance of 0.7 ± 0.02 at 734 nm, and then mixed with 1 mL of the ABTS solution and 0.02 mL of the diluted sample at each concentration. Absorbance was measured at and after 734 nm. The radical scavenging activity of ABTS was expressed as a percentage by the following equation compared to the control without the sample.

(B: Absorbance of control / A: Absorbance of sample)

In addition, the radical scavenging activity of each extract was expressed as an IC ₅₀ value, which is the concentration of the extract necessary to reduce the absorbance of the control group without the extract to 1/2.

Linoleic acid And β- carotene of coupled oxidation Antioxidant activity against

60 mg of linoleic acid, 10 mg of β-carotene, 200 mg of Tween 80 and 10 mL of chloroform were concentrated and dissolved in 50 mL of distilled water. After mixing 1 mL of the mixed solution, 2 mL of distilled water, and 0.05 mL of ginger extract (20 mg / mL EtOH), the solution was taken out at 40 ° C. water bath for 24 hours, and the absorbance was measured at 470 nm. The antioxidant activity was computed from the absorbance value measured using the absorbance value just after manufacture as 100. In this case, 0.05 mL of distilled water instead of ginger extract was used as a control.

The results of measuring the DPPH and ABTS radical scavenging activity of ginger extracts by supercritical extraction temperature and pressure are shown in Table 3 below. DPPH, exhibited the ABTS radical scavenging activity As both increase with the highest IC ₅₀ value and the temperature at 100 bar exhibited a higher IC ₅₀ values, especially 55 ℃, 100 bar, respectively 1641.0, the lowest antioxidant activity to 1156.7 μg / mL . In the range of 200-400 bar, 677.3-721.6 μg / mL and 392.5-434.7 μg / mL, respectively, showed no significant antioxidant activity. Meanwhile, the results of measuring the antioxidant activity of the coupled oxidation of linoleic acid and β-carotene are shown in FIG. 3. The control group without the ginger extract had an antioxidant activity of 71.1% after 2 hours, indicating that oxidation occurred rapidly in a short time. When the ginger extract was added to the treatment, the difference was 84.4 ~ 99.3%, indicating that the oxidation was significantly inhibited compared to the control. In addition, the control showed 52.5% antioxidant activity even after 24 hours, whereas ginger extract showed 57.3 ~ 77.8% antioxidant activity. Like DPPH and ABTS radical scavenging activity, it showed the lowest antioxidant activity at 100 bar irrespective of extraction temperature and did not show much difference in the range of 200 ~ 400 bar. The antioxidant activity results showed a tendency almost similar to the tendency of the ginger roll content of the extract described above, and it was found that the antioxidant activity of the ginger extract has a great correlation with the ginger roll content.

Antioxidant Activity of Ginger Extracts by Supercritical Extraction Temperature and Pressure Temperature (℃) Pressure (bar) IC ₅₀ (μg / ml) DPPH ABTS 35 100 811.7 546.3 200 708.8 415.8 300 677.3 405.0 400 685.4 392.5 45 100 1267.8 897.2 200 693.5 421.4 300 699.2 418.5 400 683.5 411.4 55 100 1641.0 1156.7 200 721.6 434.7 300 691.4 427.4 400 718.1 409.9

Experimental Example  5: Anti-inflammatory Effects of Ginger Extract

(1) Experimental method

1) Cell culture and cytotoxicity measurement

RAW 264.7 cell line, a murine macrophage cell line, was distributed from Korea Cell Line Bank (KCLB, Seoul, Korea), and DMEM medium containing 10% FBS (fetal bovine serum) and 1% antibiotic (penicillin / streptomycin) 2 to 3 times and cultured for a week in the passage 37 ℃ incubator of 5% CO ₂ is present using.

To measure cytotoxicity by MTT method, RAW 264.7 cells 1 × 10 ⁵ cells / well were dispensed into 96 well plates and incubated in 37 ° C., 5% CO ₂ incubator for 24 hours. The cultured cells were replaced with a serum free medium, and then treated with LPS (100 ng / mL) and samples (Examples 1, 2, and Comparative Example) for 24 hours, and then 5 mg / mL MTT. 10 μL of the solution was added to each well and incubated for 4 hours in an incubator. After the incubation period, the supernatant was removed and 100 μL of DMSO was added to each well to dissolve the formazan crystals. The absorbance was measured at 550 nm using a microplate reader. It is expressed as a percentage for.

Cytotoxic activity against rat cell lines (RBL-2H3) was also tested by MTT method. The cell line RBL-2H3 cells used in this experiment were tested by subcultured through the pre-cultured through the Korea Cell Line Bank (Seoul, Korea). The medium used for cell culture was 5% CO ₂ at 37 ° C. using MEM medium containing 10% FBS (fetal bovine serum) and 1% antibiotic (penicillin / streptomycin). It was used after incubating for 2-3 days in an incubator.

2) NO (Nitric Oxide) production amount measurement

In order to measure the NO (Nitric Oxide) generation inhibitory ability of the sample, 100 μL of the wells or extracts of each sample (Example 1, Example 2, Comparative Example) or LPS-treated RAW 264.7 cell line for nitrite measurement 96 well Taken to plate. After adding the same amount of Griess reagent and reacting for 10 minutes, the absorbance was measured at 540 nm using a microplate reader. The concentration of nitrite was calculated by comparison with a standard straight line obtained using sodium nitrate (NaNO ₂ ).

In addition, the mast cell line RBL-2H3 was sensitized in 1 ml (2 x 10 ⁵ cells, 24 well plates) with anti-DNP IgE (0.45 μg / ml) for 16 hours and activated with DNP-BSA (10 μg / mL). 20 ml of the concentration-specific sample was treated at 37 ° C. for 30 minutes before. After completion of the reaction, 25 μl of the supernatant obtained by centrifugation at 400 × g for 10 minutes at 4 ° C. was reacted with 25 μl of substrate p-nitrophenyl-N-acetyl-bD-glucosaminide at 37 ° C. for 1 hour, followed by a stop solution (0.1 M NaCO / NaHCO). , pH 10) 200 μl was added to stop and the absorbance was measured at 405 nm to calculate the amount of b-hexosaminidase.

(2) experimental results

1) Determination of cytotoxicity and NO production inhibitory activity of supercritical extract (ginger oil)

In order to measure the anti-inflammatory effect of ginger supercritical extract (ginger oil), NO scavenging activity of ginger oil (Example 1) extracted at 35, 45, 55 ° C. at a pressure of 100 bar was measured. As a result, a slight toxicity was observed at the concentration of 100 ㎍ / mL at 55 ° C, and the higher the extraction temperature, the lower the NO production inhibitory ability. There was no significant difference (FIG. 4). As such, high NO scavenging activity was also observed in ginger supercritical oil extracted under mild conditions of 35-55 ° C. and 100 bar.

2) Measurement of cytotoxicity and NO production inhibitory activity of supercritical gourd

The inhibitory effect on the NO production of the ginger extract of Example 2 was tested. As a result, as shown in FIG. 5, cytotoxicity was observed at high concentrations in proportion to the extraction temperature, and the NO scavenging activity also showed a tendency to increase with a temperature increase, indicating a good correlation with the ginger extract of Example 1. Since the concentration-dependent high inhibition rate in the concentration range of 10-50 μg / mL, together with the ginger extract of Example 1 is expected to have a high effect on the supercritical thin anti-inflammatory.

3) Measurement of Cytotoxicity and NO Production Inhibition of Ginger Extract

The inhibitory effect on the NO production of the ginger extract of the comparative example was tested. As a result, as shown in FIG. 6, cytotoxicity was not shown, and NO scavenging ability was increased in a concentration-dependent manner (FIG. 6).

4) Cytotoxicity and NO production inhibitory activity of Gingerol

As a result of testing the inhibitory effect on ginger's NO production, known as ginger's active ingredient, it showed no cytotoxicity up to 100 μg / mL and showed about 50% NO inhibition at 10 μg / mL (FIG. 7).

As described above, the present invention not only removes or supplements ginger's unique taste and aroma by supercritical extraction under specific conditions, but also obtains an extract having a high content of gingerol (gingerol) while increasing the extraction yield in a short time. It is a very useful invention for the food industry.

In addition, the present invention can obtain a ginger extract with high anti-inflammatory activity by utilizing the ginger foil remaining after the supercritical extraction.

Although the preferred embodiments of the present invention have been illustrated and described above, the present invention is not limited to the above-described embodiments, and the general knowledge in the field of the present invention without departing from the gist of the present invention as claimed in the following claims. Anyone with a variety of modifications are possible, of course, such modifications are within the scope of the claims.

Claims (8)

(S1) preparing a ginger powder; And
(S2) Ginger powder is extracted by supercritical extraction for 1 to 3 hours at a flow rate of 1 to 3 mL / min at a temperature of 35 to 55 ° C. and a pressure of 100 to 400 bar using supercritical carbon dioxide. Ginger extract manufacturing method comprising a.
The method of claim 1,
Ginger powder in the step (S1) is a ginger extract manufacturing method having an average particle size of 50 ~ 500 μm.
The method of claim 1,
Ginger extract manufacturing method with an extraction yield of 2.5 to 3.0% by weight.
The method of claim 1, wherein after step (S2)
(S3) adding remaining oil after ginger oil is added 5 to 15 times (w / v) of 60 to 90% spirits and weighed for 6 to 36 hours at room temperature; And
(S4) filtration and concentration; ginger extract manufacturing method further comprising.
Ginger extract prepared according to the method of any one of claims 1 to 4.
The method of claim 5,
Ginger extract of 21 to 25% by weight of gingerol.
The method of claim 5,
Ginger extract with 50-98% NO (Nitric Oxide) production inhibitory effect at the concentration of 10 ~ 100㎍ / mL.
Anti-inflammatory composition comprising a ginger extract of claim 5.

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