KR101582197B1 - Preparation method of ginger with increased shogaol content - Google Patents

Abstract

The present invention relates to a method of high-pressure treatment of ginger to increase the content of ginger oil, and to a ginger having increased showol content thereby prepared. According to the method of the present invention, gingerol in the ginger can be converted economically and efficiently into showgar in a short time, and thus the increased showgar can be used for various purposes such as improvement of cognitive function, ≪ / RTI >

Description

TECHNICAL FIELD [0001] The present invention relates to a method of manufacturing a ginger having an increased content of all-

The present invention relates to a method of high-pressure treatment of ginger to increase the content of ginger oil, and to a ginger having increased showol content thereby prepared.

Ginger (Zingiber officinale Roscoe) is a genus Zingiberaceae plant belonging to the genus Zingiberaceae . It is widely distributed in Southeast Asia and has been used as a medicinal drug for folk remedies. The ginger contains 40 ~ 60% of whole starch, and it has oriental sinciness component, resin protein, fiber, pentanoic acid and minerals. The ginger has a hot spice such as zingeron, gingerol, Shogaol, dihydrogingerol, and oriental components such as citral and camphene. Antioxidative, anti-inflammatory, insecticidal and uterine relaxation activities of ginger have been reported.

Among the components of the hot spicy taste of ginger, the formula is C ₁₇ H ₂₄ O _{3, which} has anticancer and antioxidant activity and has been reported to inhibit the growth of neural tumor cells. In particular, the two components of Gingerol and Shaw show that active oxygen is removed at the stage before the gene is damaged by reactive oxygen species. In other words, ginger has excellent antioxidant effect to remove active oxygen, it blocks antioxidant effect by active oxygen, and has anticancer effect, fever, pain and strong anti-inflammatory effect. In addition, Gingerol and Showa come to calm the central nervous system, stimulate the gastric mucosa, increase gastric secretion, promote digestion and suppress vomiting. The show is more effective against arthritis due to its stronger anti-inflammatory and analgesic effects than gingerol, and it is known that ginger ingredients such as gingerol and shogaol have powerful sterilizing action.

Korean Patent Laid-Open Publication No. 2010-0056020 discloses a composition for promoting learning or memory enhancement comprising ginger extract or showol, and Korean Patent Publication No. 2010-0060124 discloses a composition for improving ischemic brain including ginger extract or showol A pharmaceutical composition for the prevention or treatment of vascular disease, and Korean Patent Laid-Open Publication No. 2010-0060123 disclose a pharmaceutical composition for preventing or treating Parkinson's disease including ginger extract or shogaol, respectively.

Japanese Unexamined Patent Application Publication No. 2012-249553 has proposed a technique for converting gingerol to sucrose by heating ginger under specific conditions.

However, there is still a need to develop a technology that will convert ginger rolls into show galleries more efficiently and quickly.

Therefore, the inventors of the present invention have conducted intensive studies in order to meet the above-mentioned development needs. As a result, they have found that gingerol can be converted into sucralose with high efficiency in a short time when high pressure treatment, especially subcritical treatment, Thus completing the present invention.

According to the method of the present invention, it is possible to economically and efficiently convert gingerol to gingerol in a short time, and thus, the amplified ginger can be used for various purposes such as improvement of cognitive function, ≪ / RTI >

FIG. 1 is a graph showing changes in the content of 6-gingerol and 6-showol in ginger subjected to heat treatment at different application temperatures and times.
2 is a graph showing changes in the content of 6-gingerol and 6-showol in heat treatment time at a constant temperature (120 ° C).
FIG. 3 is a graph showing the contents of 6-gingerol and 6-showol contents in ginger according to temperature and time under subcritical conditions.
FIG. 4 shows a reaction surface graph of the content of 6-gingerol and 6-showol in the subcritical ginger.
FIG. 5 shows optimum subcritical conditions for 6-show all amplification using the center synthesis method.

Hereinafter, the present invention will be described in detail. However, it should be understood that the present invention may be embodied in many other specific forms without departing from the spirit or essential characteristics thereof.

In one aspect, the invention relates to a method of high-pressure treatment of ginger to increase the content of ginger in the ginger. By high pressure treatment of ginger, gingerol is converted to showgol and the content of ginger ginger is increased.

Gingerol is the most common ingredient in fresh ginger, and there are several ginger rolls with different chain lengths. Gingerol is structurally unstable to heat due to the presence of? -Hydroxy keto groups. Therefore, when ginger is dried and heated, the hydroxyl group of gingerol is removed by the dehydration reaction and converted to showol having a double bond at the 4- and 5-positions.

In a preferred embodiment of the invention, the gingerol is 6-gingerol and the showgar is 6-showgar. The structural formula of 6-sucrose is shown below.

[Chemical Formula 1]

The content of 6-gingerol and 6- showol in ginger is known to vary inversely with the degree of exposure to heat during processing and storage. In the comparative example of the present invention, it was confirmed that the content of 6-gingerol decreased and the content of 6-showol increased with heating temperature and time.

In the present invention, it has been confirmed that the high-pressure treatment, particularly the subcritical treatment, can more efficiently and quickly amplify 6- That is, in a preferred embodiment of the present invention, the high pressure treatment is treated under subcritical conditions.

In one embodiment, the subcritical condition is preferably set to a pressure of at least 3 MPa using carbon dioxide (CO ₂ ) in terms of conversion efficiency. In the embodiment of the present invention, experiments were conducted at a pressure of 6.4 MPa.

The conversion of gingerol to sucrose by using the subcritical technology of the present invention is more environmentally friendly because the time and energy consumed are reduced compared with the existing physical heat treatment techniques such as steam, The show can be obtained efficiently.

The amplified amplification products according to the method of the present invention can be applied to a wide variety of applications, particularly those known to be known or to be announced in the future. For example, it can be used as a pharmaceutical composition for improving brain cognitive function, a health functional food, and the like.

The present invention will be described in more detail with reference to the following examples. However, the following examples are for the purpose of illustrating the present invention and are not intended to limit the scope of the present invention.

[Example]

Samples and reagents

The ginger used in the examples was purchased from the Garak-dong agricultural and marine products market in Seoul, South Gyeongsang Province in 2012, and the surface soil was washed and completely used.

All of the reagents used for the extraction and analysis were made with special grade reagents, and the standard materials, Gingerol and Showa all, were manufactured by Chromadex USA.

Comparative Example : Heat treatment

Ginger samples were prepared at different heating temperatures as a method to increase the content of 6 - sucrose in ginger tissue. The washed ginger was cut into pieces of not more than 1 cm and placed in a nonwoven fabric net, and heat was applied for a predetermined time at 85 ° C, 100 ° C, 120 ° C and 140 ° C.

Specifically, the group treated at 85 캜 was heat-treated for 24 hours in a hot air drier adjusted to 85 캜. In the group treated at 100 ℃, the ginger was cut into a nonwoven fabric net, put into a steamer, and heated with steam for 2 hours. At 120 ° C and 140 ° C, cut ginger was placed in an autoclave in a nonwoven fabric net, heated, and heated for 2 hours after reaching the temperature. After heating for a certain period of time, each ginger sample was cooled and dried in a hot air drier at 25 ° C, stored until no weight change, and then crushed to a size of 100 mesh (149 μm) and stored in a cool dark place.

In order to investigate the effects of heating time on the amplification of 6-show, the gingival ginger was placed in a non-woven fabric net and heated at a constant temperature of 120 ° C for 30 minutes, 90 minutes, 120 minutes and 240 minutes, Cooled, dried and pulverized in a dryer.

Example  : Subcritical  process

The subcritical environment was applied to the ginger at 90.5% moisture content and the change with time was observed.

Response surface methodology (RSM) was applied to generate an approximate response surface model for the experimentally obtained response variables from the design variables (design varisbles) in order to efficiently calculate the optimal conditions. MINITAB ^® Release 14 (manufactured by Minitab) and Maple 7.00 (manufactured by Waterloo Maple) software were used for the experimental design and the graphic production of the obtained results. The temperature (C, X ₁ ) and the time (minute, X ₂ ) in the subcritical condition were set as independent variables and they were encoded into 5 steps (-1.414, -1, 0, 1, 1.414) (central composite design, CCD) (Table 1).

The number of experimental groups was set to 13 by the central synthesis plan so that the two factor variables had 5 levels. The dependent variables affected by independent variables were 6 - gingerol (mg / g, Y ₁ ) and 6 - Show is set to (mg / g, Y ₂ ). The decimal point of the theoretical value was converted into the value close to the application and the experiment was performed.

The prepared ginger samples were placed in an extractor bath, sealed, and CO ₂ gas was injected to set the subcritical environment at a pressure of 6.4 MPa. The reaction was carried out according to the conditions of Table 1 according to the central synthesis plan, followed by drying at 45 ° C in a hot-air drier until the weight of the ginger was constant, followed by grinding into 100 mesh (149 μm).

The characteristics of the gingiva at various temperature and time were statistically analyzed by response surface regression analysis using the MINITAB program. The dependent variables (6-gingerol, 6-show) for the independent variables X _i and X _j are represented by the second-order regression equation, where β ₀ is a constant and β i, β iii and β ij represent regression coefficients .

In the same way, the optimization of the subcritical treatment condition of ginger was determined by setting the target value for each reaction variable using the MINITAB program and selecting the optimal combination of the factors maximizing the satisfaction (D) while satisfying the range.

6- Showgirl  Analysis of content change

Extract preparation

The extracts for the analysis of 6-gingerol and 6-showol content of ginger powder samples dried and pulverized after heat treatment and subcritical treatment were extracted using an ultrasonic wave extractor (Sonics & Materials). That is, 100 mL of methanol was added to the ginger powder, and the probe of the extractor was immersed in the sample mixture. After that, the sample was subjected to pulsation (on: off) at a rate of 20% . 1 for filtration.

6- Gingerol  And 6- Show is coming  Content analysis

High performance liquid chromatography (HPLC: Jasco) was used for quantitative analysis of 6-gingerol and 6-showol as the indicator components of ginger among the extracted extracts. The reversed phase column Eclipse XDB-C ₁₈ was used for analysis, and the detailed analysis conditions are shown in Table 2 below.

For quantitative analysis, 6-gingerol, 8-gingerol, 10-gingerol, 6-showol, 8-showol and 10-showol were diluted to concentrations of 10, 25, 50, Curve was drawn and the analytical value of correlation coefficient (R ² ) 0.99 or more was adopted.

Heat treatment result

FIG. 1 shows the results of measuring the content of 6-gingerol and 6-sucrose by heating at different fresh ginger temperatures. In addition, the contents of 6-gingerol and 6-showol in the ginger tissues according to the heat treatment time were analyzed and the results were shown in Fig.

Gingerol can be converted by retro-aldol reaction (conversion to gingerol and aliphatic aldehyde) and dehydration (conversion to showalol), and the sucrose is known to polymerize at high temperatures. Therefore, when ginger was heat treated for 240 minutes, it was considered that gingerol was converted into gingerol and other substances other than sucrose or 6-gingerol and 6-showol due to polymerization of 6-showol.

Subcritical  Processing result

In the subcritical application experiment, the CO ₂ pressure was maintained at 6.4 MPa for 95 to 265 minutes in the reactor to determine the retention time of the reactor at the set temperature. Using a subcritical environment setting device, 6- (3-hydroxyphenyl) -6-methylpentanoic acid was prepared by changing the application temperature (70 ° C., 80 ° C., 100 ° C., 120 ° C., 130 ° C.) and the application time (95, 120, 180, 240, The content of gingerol and 6-sucrose was measured and shown in Fig.

As shown in FIG. 3, at the subcritical treatment at 95 ° C for 95 minutes, 6-sucrose was found to be as low as 0.29 mg / g on the basis of dry ginger. When the same time was applied, the 6-gingerol decreased and the 6-show tended to increase with increasing temperature. In contrast, as the temperature increased and the time elapsed, the 6-gingerol content decreased. At the temperatures of 70 ° C, 80 ° C and 100 ° C, the structural conversion of 6-sucrose tends to increase as the application time increases. However, at 120 ° C and 130 ° C, - The content of Shaw Gaelic decreased again. This shows that the boiling point of 6-sucrose is very stable in the heat at 427.5 ℃ from atmospheric pressure, but it is considered that the structure of 6-showol is collapsed by receiving energy of 150.3 ℃ or more in the subcritical environment above a certain temperature. 6-showol content was the highest at 4.42 mg / mL when applied at 130 ° C for 95 min. When 265 min was applied at 130 ° C, it was found that most of the gingerol was converted to 6-showol there was.

Subcritical  Optimum application condition setting

6-gingerol content was set to the lowest value (0.1 mg / g) and the 6-showgar content was set to the highest value (3.9 mg / g) in order to give priority to the purity of 6- The results were analyzed.

Oh in critical conditions Temperature (℃, X ₁₎ and application time (min, X ₂₎ that are affected depend upon a threshold applied to ah variable factors of ginger cloves by controlling the variables Y ₁ (6- jinjeorol content, mg / g Dried ginger) and Y ₂ (6-showol content, mg / g dry ginger) are shown in Table 3 below.

 The highest content (4.82 mg / g) was obtained at the lowest heating temperature and the shortest heating time (70 ℃ and 95 min) for 6 - gingerol, and the highest content (0.00 mg / g). In the case of 6 - sucrose, the lowest content (0.29 mg / g) was shown at 70 ℃ and 95 min, and the highest content (4.42 mg / g) was obtained at 130 ℃ and 95 min.

Table 4 also shows the response surface model equation for Y ₁ (6-gingerol content) and Y ₂ (6-showol content), and the coefficient of determination (R ² ) is 0.994 , 0.996 and significance was recognized within 1%.

The effect of application temperature (X ₁ ) and application time (X ₂ ) on the content of 6-gingerol and 6-showol in subcritical application was shown graphically using Maple software to visually show the effect ). The relationship between the applied temperature (X ₁ ) and the time (X ₂ ) in the subcritical condition of chopped ginger showed a tendency that the 6 - gingerol decreased as the temperature increased. 6 - Gingerol did not decrease significantly with increasing time at low temperature, but showed a tendency to decrease even slightly with increasing time at high temperature. The correlation between the application temperature (X ₁ ) and the application time (X ₂ ) of the 6-show all increases gradually as the time increases. As the temperature increases and the time increases, Was rapidly increased.

The center synthesis method was performed based on the results of Table 3 so as to set the optimum 6-show all amplification conditions of ginger in the subcritical condition, and the results are shown in FIG.

As a result of the central synthesis method, the optimum value of the reaction variable was set to 3.9 mg / g for the dependent variable Y ₂ (6-sucrose, mg / g) The application temperature (X ₁ ) was 130 ° C and the application time (X ₂ ) was 204 minutes. As a result, the predicted contents of 6-gingerol and 6-showol were 0.13 mg / g and 3.85 mg / g on dry ginger, respectively, and the satisfaction was 0.99.

As a result of analysis of the ginger subcritical treatment obtained when carbonated ginger was put into subcritical equipment and CO ₂ gas was injected and treated at 130 ° C for 204 minutes under a pressure of 6.4 MPa, And 6-sucrose were contained in an amount of 13 mg and 385 mg, respectively.

Claims (6)

A method of using a ginger high pressure treatment under the subcritical condition to increase my show is coming (shogaol) content of ginger, wherein the sub-critical condition is carbon dioxide a temperature state of at least 3 MPa pressure and 100 to 130 ℃ using (CO ₂₎ ≪ / RTI > wherein the amount of the ginger in the ginger is increased. The method according to claim 1,
A method of increasing the content of olive oil in a ginger, characterized in that the gingerol is converted to a showol and the content in the ginger is increased. 3. The method of claim 2,
Characterized in that the gingerol is 6-gingerol and the showa gum is the 6-show gum. delete delete A ginger having an increase in the content of ologlycerol according to the method of any one of claims 1 to 3.

Images