KR20110137090A - Light transmittable temperature control device and polymerase chain reaction apparatus with the same - Google Patents

Abstract

PURPOSE: A real-time PCR reactor is provided to effectively control temperature of a container and to detect nucleic acid amplification in real time. CONSTITUTION: A light transmittable temperature control device(100) comprises: at least one container(110) for containing a sample therein; and a temperature control unit which guides light to irradiate light to the container and controls temperature of the container. The temperature control unit has a thermoelectric element block for heating and cooling the container.

Description

Light transmitting type temperature control device and real-time detection type polymerase chain reaction device having same {Light transmittable temperature control device and polymerase chain reaction apparatus with the same}

Embodiment of the present invention relates to a light transmission type temperature control device and a real-time detection type polymerase chain reaction device having the same, and more particularly, the light transmission type that is effective in the temperature control of the vessels for nucleic acid amplification and the real time detection of nucleic acid amplification It relates to a temperature control device and a real-time detection type polymerase chain reaction device having the same.

Nucleic acid (DNA, RNA) amplification techniques are widely used for R & D and diagnostic purposes in the life sciences, genetic engineering, and medicine fields. Among various nucleic acid amplification techniques, polymerase chain reaction (PCR) is used. Nucleic acid amplification techniques are widely used. Polymerase chain reaction (PCR) is used to amplify specific sequences in the genome as needed.

The polymerase chain reaction proceeds with a series of temperature enzyme reaction steps such as denaturation, annealing, and extension, each of which must be carried out at a constant temperature range to obtain high yield of high quality nucleic acid. Can be obtained.

A real time PCR (real time PCR) device that can monitor the product amplified by the polymerase chain reaction in real time, emits fluorescence generated by irradiating the excitation light to the sample during the amplification reaction of the sample. It is a device that detects light in real time.

Typically, the excitation light beam is brighter than the fluorescence. If the excitation light and the fluorescence are both introduced into the detection device for detecting the fluorescence, the noise contained in the signal increases, so that normal fluorescence detection is difficult. In order to reduce noise, efforts have been made to prevent the excitation light from entering the sensing device by installing various types of optical filters and lens units or adjusting an angle in the incident path of the light source. However, in this case, there is a problem in that the configuration of the optical system is complicated or the optical path is too long to increase the size of the PCR device.

An object of the present invention is to provide a real-time detection polymerase chain reaction apparatus having a simple configuration and can minimize the optical path for nucleic acid amplification.

Another object of the present invention is to provide a real-time detection type polymerase chain reaction apparatus capable of realizing precise detection performance by minimizing noise caused by excitation light rays.

Still another object of the present invention is to provide a light transmitting type temperature control device and a PCR device having the same, in which temperature control of containers for nucleic acid amplification can be performed quickly and effectively.

The light transmission type temperature control device according to the present invention comprises at least one container made of a light transmitting material and capable of accommodating a sample therein; A temperature control part for controlling the temperature is provided.

In the present invention, the temperature control unit may have at least one through hole for receiving at least a portion of the container and may include a thermoelectric element block for heating or cooling the containers.

In the present invention, the light transmission type temperature control device may further include a heat sink of heat transfer material having at least one through hole at a position corresponding to the through hole of the thermoelectric element block and in contact with one side of the thermoelectric element block. .

In the present invention, the temperature control unit may include electrodes of a transparent material that receives at least a portion of the container and generates heat when a current is applied.

In the present invention, the light transmitting temperature control device may further include a heat sink of a heat transfer material having at least one through hole for receiving at least a portion of the container.

In the present invention, the temperature control unit is provided with at least one through hole to receive at least a portion of the container and a thermoelectric element block for heating or cooling one side of the vessel, and a thermoelectric element block for supporting the lower end of the vessel made of a transparent material The heating layer may be provided with a transparent layer disposed on one side of the electrode and an electrode formed on the transparent layer to generate heat when a current is applied.

In the present invention, the light transmission type temperature control device may further include a heat sink of heat transfer material having at least one through hole at a position corresponding to the containers and disposed on one side of the heating block to transfer heat.

In the present invention, the temperature controller may include a transparent layer made of a transparent material and having at least one receiving groove into which containers are inserted, and an electrode installed on the transparent layer to generate heat.

In the present invention, the light transmission type temperature control device may further include a heat sink of heat transfer material having at least one through hole at a position corresponding to the containers and disposed on one side of the transparent layer to transfer heat.

In the present invention, the temperature control unit may cool the container using air.

The real-time detection type polymerase chain reaction apparatus according to the present invention is made of a light transmitting material and accommodates at least one container and at least a portion of the container therein to accommodate a sample therein, and guides the light so that light is irradiated to the container. A light transmission type temperature control device having a temperature control unit for controlling the temperature of the container, a light generation unit disposed on one side of the light transmission type temperature control device and irradiating light toward the containers, and disposed on the other side of the light transmission type temperature control device And a light detector for detecting fluorescence generated from the containers.

As described above, the light transmission type temperature control device and the real time detection type polymerase chain reaction device of the present invention have a temperature control unit for controlling the temperature of the containers containing the sample so that light can pass through the containers to allow for nucleic acid amplification. Temperature control of the vessels and real time detection of nucleic acid amplification can be achieved effectively.

In addition, by placing the optical elements to reduce the noise on the path of the light traveling through the container in the light transmission type temperature control device it can minimize the effect by the noise. This eliminates the need to modify the path of the light or to complicate the optical elements in order to reduce the noise flowing into the light detector, thereby simplifying the configuration of the PCR device and minimizing the optical path.

1 is a side cross-sectional view schematically showing the components of a real-time detection type polymerase chain reaction device (PCR device) according to an embodiment of the present invention.
FIG. 2 is a side cross-sectional view illustrating components of a light transmitting temperature control device provided in the PCR device of FIG. 1.
3 is a side cross-sectional view of a light transmitting temperature control device according to another embodiment of the present invention.
4 is a side cross-sectional view of a light transmitting type temperature control device according to still another embodiment of the present invention.
5 is a side cross-sectional view of a light transmitting type temperature control device according to still another embodiment of the present invention.

Hereinafter, the configuration and operation of the light transmitting type temperature controlling device and the real time detection type polymerase chain reaction device according to the present invention will be described in detail with reference to the accompanying drawings.

1 is a side cross-sectional view schematically showing the components of a real-time detection type polymerase chain reaction device (PCR device) according to an embodiment of the present invention.

The real-time PCR apparatus according to the embodiment shown in FIG. 1 controls the temperature of the containers 110 for receiving a sample and transmits light to the containers 110, and the containers 110. The light generating unit 10 for irradiating light toward the () and the light detection unit 20 for detecting the fluorescence generated from the containers (110).

In determining the degree of amplification for nucleic acid amplification, a technique of confirming the degree of amplification of a nucleic acid sample by electrophoresis has been conventionally used after all amplification processes are completed. According to this technique, the amplification degree of the nucleic acid sample cannot be confirmed in real time during the amplification process.

The real-time PCR apparatus according to the embodiment shown in FIG. 1 controls the temperature conditions of the containers 110 to amplify a nucleic acid sample to be amplified, and simultaneously detects fluorescence generated by irradiating light to the containers 110. The degree of amplification can be monitored in real time.

The light generator 10 is disposed on one side of the light transmission type temperature control device 100 to irradiate light toward the containers 110 containing the nucleic acid samples. The light detector 20 may include a light source 11 that generates light, a planarizing lens 12 that flattens the light of the light source 11, and light having a wavelength in a specific range of regions. An excitation filter 13 for passing through, an enlarged lens 14 for enlarging the filtered light, and a refraction unit 15 for refracting the direction of the light with the light transmission type temperature control device 100. do.

As the light source 11, a plurality of LEDs, LED arrays, lasers, halogen lamps, or other suitable light generating devices can be used.

The light irradiated from the light source 11 to the light transmitting type temperature controller 100 passes through the flattening lens 12 and undergoes a flattening process. Thereafter, the light passes through the first wavelength selection filter 13 and is enlarged by the magnifying lenses 14 to be uniformly irradiated to the containers 110 of the light transmission type temperature control device 100. Since the containers 110 are made of a light-transmissive material, light passes through the containers 110 and enters the samples contained in the containers 110. When light is irradiated onto the containers 110, emission light is generated from the fluorescent label included in the sample.

The fluorescence generated in the containers 110 is imaged to the light detector 20 by the imaging optical system 30. The imaging optical system 30 includes lenses 31 and 32 for condensing fluorescence emitted from the light transmission type temperature control device 100 and a second wavelength selective filter for passing light having a wavelength in a specific range in the emitted fluorescence. 33 is provided. The second wavelength selection filter 33 may minimize the influence of noise (noise) by blocking the excitation light beam and transmitting only the generated fluorescence.

In the imaging optical system 30, a microscope objective lens may be used instead of a telecentric lens, and thus, a fluorescent microscopy capable of observing the amplification process of nucleic acids on a molecular basis may be implemented.

The light detector 20 may be implemented by using a photodetector such as a photodiode, a CCD device, or a CMOS device. The light detector 20 receives the fluorescence emitted from the light transmission type temperature control device 100 and generates an electrical signal corresponding thereto. Therefore, the amplification degree of the nucleic acid sample to be amplified can be quantitatively determined in real time from the electrical signal of the light detector 20.

Polarizers may be disposed on the path of light from the light generator 10 to the light detector 20 to reduce the influence of noise.

FIG. 2 is a side cross-sectional view illustrating components of a light transmitting temperature control device provided in the PCR device of FIG. 1.

The light transmissive temperature control apparatus 100 according to the embodiment shown in FIG. 2 includes a plurality of containers 110 made of a light-transmissive material and capable of accommodating a sample therein, and the temperature of the containers 110. The temperature control part 120 is provided.

Light transmitting type temperature control device 100 is implemented as a device called a thermal cycler (thermal cycler), the temperature control unit 120 has a function of heating or cooling the temperature of the vessels 110 in accordance with the cycle required for nucleic acid amplification Can be performed. The present invention is not limited by the technology for implementing the light transmitting type temperature control device 100 as a thermal cycler as described above, and by modifying the light transmitting type temperature control device 100 to operate as an isothermal block. It may be used to implement an isothermal nucleic acid amplification apparatus and its isothermal target & probe amplification.

Since the containers 110 are made of a material such as transparent plastic or glass, light may penetrate the containers 110. The nucleic acid samples to be amplified can be accommodated in the containers 110. The tops of the containers 110 are supported by the support plate 115. The containers 110 are inserted into the thermoelectric element block 121 of the temperature controller 120.

The temperature controller 120 includes a thermoelectric element block 121 disposed outside the vessels 110 to control the temperature of the vessels 110, and a controller 122 that controls the thermoelectric element block 121. . The thermoelectric element block 121 may be implemented by, for example, a peltier element.

The Peltier device is an electric / heat conversion device using the Peltier effect. The Peltier device uses a thermoelectric phenomenon in which heat is generated or heat is absorbed at a junction of two metals when a current is connected to two kinds of metals. Therefore, the controller 122 applies the current to the thermoelectric element block 121 through the electrical wiring 122a to control the temperature of the vessels 110 by causing the temperature of the thermoelectric element block 121 to rise or fall. can do.

The controller 122 may be implemented with, for example, a semiconductor chip or a circuit board using the semiconductor chip.

The temperature sensor 127 may be installed in the thermoelectric element block 121, and the controller 122 may control the temperature of the thermoelectric element block 121 based on a detection signal of the temperature sensor 127.

The controller 122 also performs the function of transmitting light through the containers 110, for which the thermoelectric element block 121 has a plurality of through holes 121a into which the containers 110 are inserted. do. The through holes 121a of the thermoelectric element block 121 are in contact with the side surfaces of the containers 110 and support the containers 110, and an optical path through which light incident from the outside is introduced into the containers 110. Function as.

The light transmission type temperature control device 100 may further include a heat sink 130 made of a heat transfer material in contact with one side of the thermoelectric element block 121. The heat sink 130 may perform a function of assisting rapid cooling by transferring heat generated from the thermoelectric element block 121 to the outside. Heat sink 130 may be made of a thermally conductive metal, such as aluminum or copper, for example. By installing the heat sink 130, a cooling effect due to the natural convection effect can be obtained. The heat sink 130 may adopt a well-known heat pipe structure in addition to the illustrated structure.

In addition to the structure of installing the heat sink 130, various technologies can be introduced to assist the cooling effect. For example, although not shown in the drawing, a separate blower for blowing air or a pipe for cooling the cooling fluid flows therein. You can also install

The light transmission type temperature control device 100 having the above-described configuration and a real-time PCR device having the same may effectively detect and amplify DNA, for example.

The PCT apparatus, which shows only the qualitative result of DNA amplified by gel electrophoresis at the end point of the conventional PCR apparatus, has many problems such as accuracy in quantitative detection of DNA. In order to solve this problem, a real-time PCR device that enables quantitative analysis of DNA by detecting the intensity of fluorescence in proportion to the concentration of amplified DNA using an optical detection system has been used. However, in the conventional real-time PCR device, both the excitation light and the fluorescence are introduced, so there is a lot of noise included in the signal, and in order to overcome such a problem, the configuration of the optical system is complicated and the optical path is too long, resulting in a large size of the PCR device. .

For DNA amplification, the template DNA to be amplified, an oligonucleotide primer pair having a sequence complementary to a specific sequence of each single strand of the template DNA, and a high temperature stable DNA polymerase And prepare a sample containing deoxyribonucleotide triphosphates (dNTP).

After injecting the prepared sample into the containers 110 of the light-transmitting temperature control device 100, a specific site sequence of the template DNA is amplified by repeating a temperature cycle of sequentially changing the temperatures of the containers 110. Specifically, three or two temperature cycling cycles are used.

The first step is the denaturation step, which separates double-stranded DNA into single-stranded DNA by heating the sample to a high temperature.

The second step is an annealing step, where the denaturated sample is cooled to an appropriate temperature, where the DNA-primer complex is partially double-stranded by double-stranded binding of single-stranded DNA and primers. Forming a step.

The third step is the polymerization step, where the primers of the DNA-primer complex are extended to the original template DNA by extension of the primers of the DNA-primer complex by the polymerization reaction by keeping the sample subjected to the annealing step at an appropriate temperature. It is a step of replicating a new single-stranded DNA having a sequence.

By repeating these three steps 20 to 40 times in sequence, DNA between two primers can be replicated every cycle, thereby achieving millions of times or more of DNA amplification.

The temperature in the denaturation step is a value in the range of 90 ℃ to 95 ℃, the temperature in the annealing step is appropriately adjusted according to the melting point (Tm) of the primer used, it is usually a value in the range of 40 ℃ to 60 ℃. The temperature in the polymerization stage is set to 72 ° C., which is the optimum active temperature of the high temperature stable Taq DNA Polymerases extracted from the commonly used Thermos aquaticus, and it is best to use a three-step temperature cycle. It is universal. Since the active temperature range of the tack DNA polymerase is quite wide, a two-stage temperature cycle is also used in which the temperature of the unwinding and polymerization steps are equalized to circulate the temperature.

Here, since the maintenance of a constant temperature and the temperature change step by step is not made quickly, when the temperature is lowered in the annealing step, the primer does not stick to the position to be amplified, which greatly affects the yield.

1 and 2 is a thermoelectric device 100 having through-holes 131 for guiding light incident from the outside so that light enters the containers 110. By using the block 121, it is possible to effectively heat and cool the vessels 110 and to effectively detect the generated fluorescence.

3 is a side cross-sectional view of a light transmitting temperature control device according to another embodiment of the present invention.

The light-transmitting temperature control apparatus 200 according to the embodiment shown in FIG. 3 includes a plurality of containers 210 made of a light-transmissive material and capable of accommodating a sample therein, and the temperature of the containers 210. The temperature control unit 220 is provided.

The temperature controller 220 is made of a transparent material and disposed on the surfaces of the containers 210 to control the temperatures of the containers 210 and to control the application of current to the electrodes 221. Has a controller 222. The electrodes 221 may be manufactured by coating the surfaces of the containers 210 using, for example, a transparent material such as a carbon nanotube (CNT) or an indium tin oxide (ITO) film. The electrodes 221 are electrically connected to the controller 222 by the electrical wiring 222a.

The temperature sensor 227 may be installed outside the containers 210, and the controller 222 may control the temperatures of the containers 210 based on a detection signal of the temperature sensor 227.

The light transmission type temperature control device 200 may include a heat sink 230 made of a heat transfer material including a plurality of through holes 231 into which the containers 210 are inserted. The heat sink 230 may transfer heat generated from the electrodes 221 on the surfaces of the containers 210 to the outside to assist in rapid cooling. Heat sink 230 may be made of a thermally conductive metal, such as aluminum or copper, for example.

4 is a side cross-sectional view of a light transmitting type temperature control device according to still another embodiment of the present invention.

The light transmissive temperature control device 300 according to the embodiment shown in FIG. 4 includes a plurality of containers 310 made of a light-transmissive material and capable of accommodating a sample therein, and the temperature of the containers 310. The temperature control unit 320 is provided.

The temperature controller 320 includes a thermoelectric element block 321 disposed outside the vessels 310 to control the temperature of the vessels 310 and a heating material for supporting the lower ends of the vessels 310 made of a transparent material. And a controller 322 for controlling the thermoelectric element block 321 and the heating block 323.

The thermoelectric element block 321 may be implemented with, for example, a peltier element. The thermoelectric element block 321 is electrically connected to the controller 322 through the first wiring 322a, and may heat or cool the containers 310 as a current is applied from the controller 322. The thermoelectric element block 321 includes a plurality of through holes 321a in which the containers 310 are accommodated.

The heating block 323 includes, for example, a transparent layer 324 made of glass or a transparent plastic material, and an electrode 325 formed in the transparent layer 324 to generate heat when a current is applied. The electrode 325 is connected to the controller 322 through the second wiring 322b.

The electrode 325 is deposited on the surface of the transparent layer 324 using a transparent material such as, for example, carbon nanotube (CNT) or indium tin oxide (ITO) film, or as shown in FIG. 4. 324 may be embedded in the interior.

The electrode 325 may be disposed outside at the position corresponding to the containers 310 in the transparent layer 324. Although the electrode 325 is manufactured to transmit light, the transmittance of light incident on the containers 310 may be increased by being disposed to avoid a path of light incident on the containers 310. When the electrode 325 is disposed to avoid the path of light, the electrode 325 does not necessarily need to be made of a transparent material, but may be manufactured using various materials having electrical conductivity such as copper or nickel.

The light transmission type temperature control device 300 may include a temperature sensor 327 disposed in the thermoelectric element block 321 or the heating block 323 to sense a temperature. Since the controller 322 is connected to the temperature sensor 327 through the third wire 322c, the controller 322 may control the temperatures of the containers 310 based on the detection signal of the temperature sensor 327.

The light transmission type temperature control device 300 may include a heat sink 330 of a heat transfer material including a plurality of through holes 331 into which the containers 310 are inserted. The heat sink 330 may transfer heat generated by the heating block 323 to the outside to assist in rapid cooling. Heat sink 330 may be made of a thermally conductive metal, such as aluminum or copper, for example.

According to the light transmission type temperature control device 300 having the above-described configuration, due to the action of the thermoelectric element block 321 and the heating block 323 it is possible to effectively control the temperature of the containers 310, the light is heat sink Through the through holes 331 of 330, the transparent layer 324 and the through holes 321a of the thermoelectric element block 321 is incident to the containers 310 to generate fluorescence to effectively detect the degree of nucleic acid amplification can do.

5 is a side cross-sectional view of a light transmitting type temperature control device according to still another embodiment of the present invention.

The light-transmitting temperature control device 400 according to the embodiment shown in FIG. 5 is made of a light-transmissive material and controls the temperature of the plurality of containers 410 and the containers 410 that can accommodate a sample therein. The temperature control unit 420 is provided.

The temperature controller 420 includes a transparent layer 421 of a transparent material having a plurality of receiving grooves 421a into which the containers 410 are inserted, and electrodes 425 installed on the transparent layer 421 to generate heat. And a controller 422.

Since the transparent layer 421 is made of, for example, glass or a transparent plastic material, the transparent layer 421 may transmit light. The electrode 425 is connected to the controller 422 through electrical wiring 422a. As a current is applied from the controller 422 to the electrode 425, the electrode 425 may generate heat. The electrode 425 is deposited on the surface of the transparent layer 421 using a transparent material such as, for example, carbon nanotube (CNT) or indium tin oxide (ITO) film, or as shown in FIG. 5. 421 may be embedded in the interior.

The electrode 425 may be disposed outside the containers 410 in the transparent layer 421. The electrode 425 is disposed to avoid the path of the light incident on the containers 410, thereby increasing the transmittance of the light incident on the containers 410, and avoiding optical interference that may occur between the containers 410. It can prevent. For example, if the electrode 425 is arranged to surround each of the containers 410, the performance of heating the containers 410 can be greatly improved.

When the electrode 425 is disposed to avoid the path of light, the electrode 425 does not necessarily need to be made of a transparent material, but may be manufactured using various materials having electrical conductivity such as copper or nickel.

The light transmission type temperature control device 400 may include a temperature sensor 427 disposed on the transparent layer 421 to sense a temperature. The controller 422 may control the temperatures of the containers 410 based on the sensed signal of the temperature sensor 427.

The light transmission type temperature control device 400 may further include a heat sink 430 made of a heat transfer material in contact with one surface of the transparent layer 421. The heat sink 430 may perform a function of transferring heat generated in the transparent layer 421 to the outside to assist rapid cooling. Heat sink 430 may be made of a thermally conductive metal such as aluminum or copper, for example. By installing the heat sink 430, a cooling effect due to the natural convection effect can be obtained.

Cooling pipes 450 through which the cooling fluid may flow may be embedded in the transparent layer 421. The cooling pipes 450 may be disposed to avoid the positions of the containers 410 so as not to disturb the inflow of light incident to the containers 410.

When the vessels 410 are cooled, the vessels 410 may be rapidly cooled by heat dissipation due to a convection phenomenon using the heat sink 430 and the action of a cooling fluid flowing through the cooling pipes 450.

Although the present invention has been described with reference to the above-described embodiments, these are merely exemplary, and it will be understood by those skilled in the art that various modifications and equivalent other embodiments are possible. Therefore, the true technical protection scope of the present invention will be defined by the appended claims.

10: light generation unit 322b: second wiring
11: light source 322c: third wiring
12: flattening lens 323: heating block
13: first wavelength selective filter 122a, 222a: electrical wiring
14: magnifying lenses 421a: receiving grooves
15: refractive portion 422a: electrical wiring
20: light detector 121, 321: thermoelectric element block
30: imaging optical system 450: cooling pipes
33: second wavelength selective filter 324, 421: transparent layer
31, 32: lenses 221, 325, 425: electrode
115: support plate 122, 222, 322, 422: controller
322a: first wiring 110, 210, 310, 410: containers
127, 227, 327, 427: temperature sensor 130, 230, 330, 430: heat sink
120, 220, 320, 420: temperature control
121a, 131, 231, 321a, 331: through holes
100, 200, 300, 400: Light transmission type temperature control device

Claims (11)

At least one container made of a light transmitting material and capable of accommodating a sample therein; And
And a temperature controller for accommodating at least a portion of the vessel, guiding the light to irradiate the vessel, and controlling the temperature of the vessel. The method of claim 1,
And the temperature control unit has at least one through hole for receiving at least a portion of the container and includes a thermoelectric element block for heating or cooling the container. The method of claim 2,
And at least one through hole at a position corresponding to the through hole of the thermoelectric element block, and further comprising a heat sink made of a heat transfer material in contact with one side of the thermoelectric element block. The method of claim 1,
The temperature control unit receives at least a portion of the container, and a light transmission type temperature control device having electrodes of a transparent material that generates heat when a current is applied. The method of claim 4, wherein
And a heat sink of a heat transfer material having at least one through hole for receiving at least a portion of the container. The method of claim 1,
The temperature control unit,
A thermoelectric element block having at least one through hole to receive at least a portion of the container and heating or cooling one surface of the containers; And
And a heating block made of a transparent material and disposed on one side of the thermoelectric element block to support lower ends of the containers, and a heating block formed on the transparent layer to generate heat when an electric current is applied thereto. Transmissive temperature control device. The method of claim 6,
And at least one through hole at a position corresponding to the containers, and further comprising a heat sink of a heat transfer material disposed on one side of the heating block to transfer heat. The method of claim 1,
The temperature control unit is made of a transparent material having a transparent layer having at least one receiving groove into which the containers are inserted, and a light transmitting type temperature control device having an electrode installed in the transparent layer to generate heat. The method of claim 8,
And at least one through hole at a position corresponding to the containers, and further comprising a heat sink of a heat transfer material disposed on one side of the transparent layer to transfer heat. The method of claim 1,
And the temperature control unit cools the vessel using air. At least one container made of a light-transmitting material and capable of accommodating a sample therein; With a light transmission type temperature control device;
A light generator disposed at one side of the light transmission type temperature control device and configured to irradiate light toward the containers; And
And a light detector disposed on the other side of the light transmission type temperature control device to detect fluorescence generated from the containers.

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