CN112760214A - Quick visual isothermal amplification device - Google Patents
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- CN112760214A CN112760214A CN202011552871.6A CN202011552871A CN112760214A CN 112760214 A CN112760214 A CN 112760214A CN 202011552871 A CN202011552871 A CN 202011552871A CN 112760214 A CN112760214 A CN 112760214A
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- B01L7/00—Heating or cooling apparatus; Heat insulating devices
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Abstract
The invention discloses a rapid visual isothermal amplification device, which comprises a reaction tube fixing mechanism, a reaction tube fixing mechanism and a temperature control mechanism, wherein the reaction tube fixing mechanism is used for fixing at least one reaction tube and heating at least one reaction tube to a target temperature so that reagents in the reaction tubes are subjected to isothermal amplification reaction at the target temperature; the detection mechanism is used for acquiring a real-time color numerical value of the reagent in the reaction tube in the isothermal amplification reaction process, and judging the attribute of the reagent according to the color numerical value. The application provides a quick visual isothermal amplification device can observe the color change in the reagent reaction process often, can take notes the color change process. And the color value reaches the threshold value, the instrument has alarm reminding, time is saved, result judgment errors are reduced, and detection is more accurate. The instrument can realize batch detection, simultaneously output 96 results every time, and has high flux and high efficiency.
Description
Technical Field
The invention relates to the technical field of nucleic acid detection equipment, in particular to a rapid visual isothermal amplification device.
Background
In recent years, detection techniques based on isothermal nucleic acid amplification have been developed rapidly. Isothermal techniques of various mechanisms have gradually matured and are widely applied in the fields of molecular biology, medicine, law and the like. Particularly, in the aspects of clinical and on-site rapid diagnosis technologies, the isothermal nucleic acid amplification technology shows outstanding advantages. More importantly, the isothermal nucleic acid amplification technology does not need time course of temperature change and gets rid of dependence on fine equipment, so that the detection and diagnosis of pathogens can be realized quickly and in high flux. In the isothermal amplification of nucleic acids, the solution undergoes an isothermal amplification reaction over time. During the reaction, the pH value of the solution changes, and the corresponding color of the solution changes. And after the reaction is completed after 30-45min, removing the sample from the isothermal amplification device, observing the color change of the sample, and then judging the conclusion of the sample.
However, in the prior art, the nucleic acid isothermal amplification instrument has long reaction time, and after a sample is separated from the isothermal amplification device, the temperature change is irregular, thereby influencing the result judgment. And errors are introduced when the human eyes observe for judgment. In addition, the existing nucleic acid detection equipment has low flux and long detection result acquisition time, and is not suitable for application places with large sample detection amount and high-efficiency detection requirements.
Disclosure of Invention
The invention provides a rapid visual isothermal amplification device. The problems that in the prior art, nucleic acid detection equipment is low in detection efficiency and needs to judge detection results manually are solved, and high-throughput, automatic and accurate-judgment nucleic acid detection is realized.
The invention provides the following scheme:
a rapid visualization isothermal amplification device, comprising:
the reaction tube fixing mechanism is used for fixing at least one reaction tube and heating at least one reaction tube to a target temperature so that reagents in the reaction tubes can perform isothermal amplification reaction at the target temperature;
the detection mechanism is used for acquiring a real-time color numerical value of the reagent in the reaction tube in the isothermal amplification reaction process, and judging the attribute of the reagent according to the color numerical value.
Preferably: further comprising:
and an adjusting mechanism for adjusting the target temperature and the reaction time of the isothermal amplification reaction according to the reaction characteristics of the reagent that change with temperature and time.
Preferably: the reaction tube fixing mechanism comprises a base and a reaction tube frame which is arranged on the base and provided with a heating module; at least one accommodating groove for accommodating at least one reaction tube is formed on the reaction tube rack.
Preferably: the holding tank includes a plurality ofly, every the holding tank all disposes different serial numbers to according to different serial numbers carry out corresponding serial numbers to the reaction tube that is located inside each holding tank.
Preferably: the bottom of each accommodating groove is provided with a through hole, and the through holes are used for enabling a part of the reaction tube to extend out of the through holes, so that the detection mechanism can obtain real-time color values of the reagents in the reaction tube in the isothermal amplification reaction process through the extending parts of the reaction tube.
Preferably: the heating module comprises a plurality of independent heating units, and each heating unit is arranged in one-to-one correspondence with at least one accommodating groove.
Preferably: still include temperature control mechanism and at least one temperature sensor, at least one temperature sensor and each heating unit respectively with temperature control mechanism can communicate and link to each other temperature sensor is used for acquireing the real-time temperature of reaction tube support, temperature control mechanism be used for with real-time temperature with the target temperature is compared to according to the heating temperature of the comparison result control each heating unit that obtains.
Preferably: the heating unit includes heater strips uniformly arranged with the accommodating groove as a reference.
Preferably: the reaction tube fixing mechanism further comprises an upper cover with a heating function.
Preferably: the reaction tube fixing mechanism further comprises a rotating motor, and an output shaft of the rotating motor is connected with the base and used for driving the base to rotate around the horizontal center of the base.
Preferably: also included is an illumination module for providing a light source to reagents within the reaction tube.
Preferably: the illumination module is positioned above the upper cover, and light beams generated by the illumination module are provided to the reagent through the upper cover and the tube cover of the reaction tube.
Preferably: the illumination module comprises a plurality of point light sources, and the point light sources are correspondingly arranged right above the reaction lights one by one so that light rays generated by the point light sources vertically irradiate on the reaction tube.
Preferably: the detection mechanism comprises an imaging module and a detection module, wherein the imaging module is used for acquiring a real-time image of the reagent in the reaction tube, and the detection module is used for acquiring a color numerical value of the reagent through the real-time image so as to judge the attribute of the reagent according to the color numerical value.
Preferably: the detection module is also used for recording the color numerical value of the reagent in each reaction tube after the color is changed and generating a detection report containing the color numerical value; the color numerical values include hue values in a color model.
According to the specific embodiment provided by the invention, the invention discloses the following technical effects:
according to the invention, a rapid visual isothermal amplification device can be realized, and in one implementation mode, the device can comprise a reaction tube fixing mechanism for fixing at least one reaction tube and heating at least one reaction tube to a target temperature, so that reagents in the reaction tubes are subjected to isothermal amplification reaction at the target temperature; the detection mechanism is used for acquiring a real-time color numerical value of the reagent in the reaction tube in the isothermal amplification reaction process, and judging the attribute of the reagent according to the color numerical value. The application provides a quick visual isothermal amplification device can observe the color change in the reagent reaction process often, can take notes the color change process. And the color value reaches the threshold value, the instrument has alarm reminding, time is saved, result judgment errors are reduced, and detection is more accurate. The instrument can realize batch detection, simultaneously output 96 results every time, and has high flux and high efficiency.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a rapid visualization isothermal amplification apparatus provided in an embodiment of the present invention.
In the figure: the device comprises a reaction tube fixing mechanism 1, a base 11, a reaction tube rack 12, an upper cover 13, a detection mechanism 2, an imaging module 21, a detection module 22, an illumination module 3 and a reaction tube 4.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments that can be derived by one of ordinary skill in the art from the embodiments given herein are intended to be within the scope of the present invention.
Examples
Referring to fig. 1, a rapid visualization isothermal amplification apparatus provided for an embodiment of the present invention, as shown in fig. 1, may include:
the reaction tube fixing mechanism 1 is used for fixing at least one reaction tube 4 and heating the at least one reaction tube to a target temperature so as to enable reagents in the reaction tube 4 to perform isothermal amplification reaction at the target temperature;
the detection mechanism 2 is used for acquiring a real-time color numerical value of the reagent in the reaction tube 4 in the isothermal amplification reaction process, and judging the attribute of the reagent according to the color numerical value.
The reaction tube fixing mechanism can enable the reaction tube fixing mechanism to generate heat through the heating component configured by the reaction tube fixing mechanism, and further transfer the heat to the reaction tube, so that the temperature of the reagent in the reaction tube is increased, and the temperature requirement of isothermal amplification reaction is met. It is understood that the target temperature may be adjusted according to the temperature required for isothermal amplification reactions of different reagents. For example, in one implementation, an adjustment mechanism may be further included for adjusting the target temperature and the reaction time of the isothermal amplification reaction according to the reaction characteristics of the reagents with respect to temperature and time. The adjustment mechanism can adjust the heating temperature and the heating time, and for example, the temperature can be raised to 65 degrees celsius and maintained for 30 minutes.
The reaction tube fixing mechanism provided in the embodiment of the present application may be manufactured in various forms, for example, the reaction tube fixing mechanism 1 may include a base 11 and a reaction tube holder 12 having a heating function and disposed on the base; at least one accommodating groove for accommodating at least one reaction tube is formed on the reaction tube holder 12. The reaction tube holder 12 may be formed integrally with the heating element or may be formed separately therefrom. In order to improve the working efficiency and simultaneously make a plurality of reaction tubes carry out isothermal amplification reaction, the holding tank comprises a plurality of holding tanks, and each holding tank is configured with different numbers so as to correspondingly number the reaction tubes positioned in each holding tank according to the different numbers.
In order to obtain the color value of the reagent in the reaction tube conveniently, a through hole is formed at the bottom of each accommodating groove, and the through hole is used for enabling a part of the reaction tube 4 to extend out of the through hole, so that the detection mechanism 2 can obtain the real-time color value of the reagent in the reaction tube 4 in the isothermal amplification reaction process through the extending part of the reaction tube 4. One part of the reaction tube 4 is leaked from the lower part of the reaction tube frame, so that the detection mechanism can be arranged at the lower part of the reaction tube fixing mechanism, and the image acquisition module acquires the leaked part to accurately obtain the real-time image of the reagent in each reaction tube.
In order to further improve the heating effect, the reaction tube fixing mechanism 1 further includes an upper cover 13 having a heating module. In order to heat the reaction tube rack evenly, the heating module comprises a plurality of independent heating units, and each heating unit is arranged corresponding to at least one accommodating groove one to one. The heating unit may include various forms, for example, the heating unit includes heating wires uniformly arranged with reference to the accommodating groove. It is understood that each heating unit can individually control the heating time and the heating temperature. In order to better realize the control to the temperature, still include temperature control mechanism and at least one temperature sensor, at least one temperature sensor and each heating unit respectively with temperature control mechanism is communicable to be linked to each other temperature sensor is used for acquireing the real-time temperature of reaction tube frame, temperature control mechanism be used for with real-time temperature with the target temperature compares to according to the heating temperature of the comparison result control each heating unit who obtains. Specifically, firstly, the heating module is divided into rectangular or regular-shaped areas, heating wires are uniformly distributed by taking the accommodating groove as a reference, meanwhile, a temperature sensor is arranged at a position where the temperature gradient can obviously change, the temperature sensor is used as the input of a temperature control system, the regular-shaped subareas of the heating module are used as the output of the temperature system, the temperature control system reads the sensor value in real time and compares the sensor value with a preset target temperature value, and the temperature value of each regular-shaped area of the heating module is controlled within an effective temperature range through PID control.
In order to ensure that the light intensity meets the requirement of clear imaging when the image acquisition module acquires images, the embodiment of the application can further provide the illumination module 3, and the illumination module 3 is used for providing a light source for the reagent in the reaction tube 4. Specifically, the illumination module 3 is located above the upper cover 13, and the light beam generated by the illumination module 4 is provided to the reagent through the upper cover 13 and the tube cover of the reaction tube 4. The embodiment of the application can provide that the lighting module comprises a plurality of point light sources, and each point light source is arranged right above each reaction light in a one-to-one correspondence manner, so that light rays generated by the point light sources vertically irradiate on the reaction tubes. The illumination of upper cover adopts the dot matrix light source to arrange, all has the point light source directly over every reaction tube, and its position guarantees that the illumination light vertically incides on the reaction tube when the upper cover is covered to guarantee that there is not the interference that the light scattering brought between the pipe.
The detection mechanism 2 can adopt any mechanism which can realize the acquisition of the image of the reaction tube, can extract the local color in the image and can compare the color with the preset color. For example, the embodiment of the present application may provide that the detection mechanism 2 includes an imaging module 21 and a detection module 22, the imaging module 21 is configured to obtain a real-time image of the reagent in the reaction tube 4, and the detection module 22 is configured to obtain a color value of the reagent through the real-time image, so as to make a judgment on an attribute of the reagent according to the color value. The imaging module may be a camera or the like. In order to better show the detection result to the user. The detection module is also used for recording the color numerical value of the reagent in each reaction tube after the color is changed and generating a detection report containing the color numerical value; the color numerical values include hue values in a color model.
In another preferred embodiment, the reaction tube fixing mechanism further comprises a rotating motor, and an output shaft of the rotating motor is connected with the base 11 and is used for driving the base 11 to rotate around the horizontal center of the base 11. The base 11 rotates to drive the reaction tube 4 on the reaction tube rack to move in the set temperature environment. The temperature of the heating unit among the prior art itself is difficult to maintain and is the same from time to time, and the department is unanimous, and consequently the ambient temperature that the heating unit on reaction tube support 12, the upper cover 13 formed is inhomogeneous, and this application can improve the uniformity of the temperature of each reaction tube 4 through reaction tube 4 motion in the environment of temperature imbalance to guarantee that experimental reaction time is unanimous as far as possible. Compare and to possess reaction tube frame 12, the upper cover 13 rotation of heating unit, this application adopts the mode that base 11 rotated, avoids the winding problem of heating unit's pipeline.
The following describes the device and its method of use in detail, taking as an example a specific structure that can form the device provided by the present application in one embodiment.
The device can comprise a reaction tube fixing mechanism consisting of a base, a reaction tube frame, an upper cover and a heating module on the base, an illuminating module and a detecting mechanism consisting of an imaging module and a detecting module; the illumination module and the detection module are respectively positioned at the upper side and the lower side of the reaction tube rack; the reaction tube frame is provided with a plurality of accommodating grooves, and light rays generated by the illumination module can sequentially pass through the upper cover, the heating module and the tube cover of the reaction tube to irradiate the reagent sample; the bottom end of the reaction tube support is provided with an opening, the imaging module receives light rays of the reagent sample, imaging is carried out on reaction tubes in all the accommodating grooves, and the detection module receives an imaging effect. The illumination module adopts a light homogenizing device, so that illumination is stable and balanced; meanwhile, the imaging effect adopts a calibration process, and the detection result is reliable.
When the device is used, the accommodating grooves on the base are numbered in advance, for example, the row index is A, B, C, D … …, and the column indexes are 1, 2 and 3 … …. Putting the processed throat swab reagent into an 8-row reaction tube, putting the reaction tube into a containing groove, numbering the reaction tube according to the number of the containing groove, namely numbering the reaction tube in the A1 groove as A1; and finishing the positive and negative detection threshold corresponding to the color of the reagent in the reaction tube according to the preset experiment. Starting a heating function to heat the hot cover and the base to a temperature value for reagent reaction; when the temperature reaches a set value, the illumination is turned on (the illumination on time point can be advanced, such as the illumination is always on or the illumination is turned on when heating is on); the imaging module and the detection module acquire the color numerical value of the reagent in the reaction tube in real time, and record the serial number of the reaction tube if the detected color value is in the color numerical value range corresponding to the preset positive threshold value. After the recording, the color value can be judged after the preset time, so that the obtained reagent attribute result is more accurate. For example, when the test value of a certain sample reaches a threshold value, an alarm is given, and after a certain fixed time (30min), the threshold value judgment result is counted again. And carrying out temperature control and time control according to the reaction characteristics of the reagent along with temperature and time, recording the color numerical values in each reaction tube in real time in the detection process, forming a detection report by the color numerical values in the reaction tubes for tracing and identification after the detection is finished, and displaying negative and positive results according to threshold setting. If the detection value of the A1 tube is X, the detection result at A1 is positive if X is within the positive threshold range. In the detection process, reagent changes in all the reaction tubes can be digitally displayed, and after detection is finished, detection conclusions of all the reaction tubes are judged and presented simultaneously, so that detection is visual and rapid.
In a word, the rapid visual isothermal amplification device provided by the application can observe color change in the reaction process of the reagent at any time, and can record the color change process. And the color value reaches the threshold value, the instrument has alarm reminding, time is saved, result judgment errors are reduced, and detection is more accurate. The instrument can realize batch detection, simultaneously output 96 results every time, and has high flux and high efficiency.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.
Claims (10)
1. A rapid visualization isothermal amplification device, comprising:
the reaction tube fixing mechanism is used for fixing at least one reaction tube and heating at least one reaction tube to a target temperature so that reagents in the reaction tubes can perform isothermal amplification reaction at the target temperature;
the detection mechanism is used for acquiring a real-time color numerical value of the reagent in the reaction tube in the isothermal amplification reaction process, and judging the attribute of the reagent according to the color numerical value.
2. The rapid visualization isothermal amplification device according to claim 1, further comprising:
and an adjusting mechanism for adjusting the target temperature and the reaction time of the isothermal amplification reaction according to the reaction characteristics of the reagent that change with temperature and time.
3. The rapid visualization isothermal amplification device according to claim 1, wherein the reaction tube fixing mechanism comprises a base and a reaction tube holder with a heating module disposed on the base; the reaction tube rack is provided with at least one accommodating groove for accommodating at least one reaction tube;
each holding tank all is configured with different serial numbers to carry out corresponding serial number to the reaction tube that is located inside each holding tank according to the serial number that differs.
4. The rapid visualization isothermal amplification device according to claim 3, wherein a through hole is formed at the bottom of each holding tank, and the through hole is used for a portion of the reaction tube to protrude from the through hole, so that the detection mechanism can obtain a real-time color value of the reagent in the reaction tube during the isothermal amplification reaction through the protruding portion of the reaction tube.
5. The rapid visualization isothermal amplification device according to claim 3, wherein the heating module comprises a plurality of independent heating units, each of the heating units being arranged in one-to-one correspondence with at least one of the holding tanks.
6. The rapid visualization isothermal amplification device according to claim 5, further comprising a temperature control mechanism and at least one temperature sensor, wherein the at least one temperature sensor and each heating unit are respectively and communicatively connected to the temperature control mechanism, the temperature sensor is configured to obtain a real-time temperature of the reaction tube rack, and the temperature control mechanism is configured to compare the real-time temperature with the target temperature and control a heating temperature of each heating unit according to an obtained comparison result.
7. The rapid visualization isothermal amplification device according to claim 3, wherein the reaction tube fixing mechanism further comprises an upper cover with a heating function.
8. The rapid visualization isothermal amplification device according to claim 3, wherein the reaction tube fixing mechanism further comprises a rotating motor, and an output shaft of the rotating motor is connected to the base for driving the base to rotate around a horizontal center thereof.
9. The rapid visualization isothermal amplification device according to claim 7, further comprising an illumination module, wherein the illumination module is located above the upper cover, the illumination module comprises a plurality of point light sources, and the point light sources are correspondingly arranged directly above the reaction tubes, so that light generated by the point light sources vertically irradiates the reaction tubes.
10. The rapid visualization isothermal amplification device according to claim 1, wherein the detection mechanism comprises an imaging module and a detection module, the imaging module is configured to obtain a real-time image of the reagent in the reaction tube, and the detection module is configured to obtain a color value of the reagent from the real-time image, so as to determine the property of the reagent according to the color value;
the detection module is also used for recording the color numerical value of the reagent in each reaction tube after the color is changed and generating a detection report containing the color numerical value; the color numerical values include hue values in a color model.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113755647A (en) * | 2021-10-15 | 2021-12-07 | 无锡市人民医院 | Novel coronavirus rapid detection system |
| CN113981139A (en) * | 2021-09-27 | 2022-01-28 | 厦门健康工程与创新研究院 | Nucleic acid isothermal amplification detection reagent, kit, detection system and method |
| CN115216553A (en) * | 2022-05-26 | 2022-10-21 | 四川国际旅行卫生保健中心(成都海关口岸门诊部) | Deep learning-based digital detection system and detection method for plasmodium falciparum |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101109706A (en) * | 2007-08-31 | 2008-01-23 | 北京工业大学 | Centrifugal type PCR analyzer |
| CN101824486A (en) * | 2009-12-30 | 2010-09-08 | 华东医学生物技术研究所 | Method and device for rapidly detecting nucleic acid |
| KR20110137090A (en) * | 2010-06-16 | 2011-12-22 | 삼성테크윈 주식회사 | Light transmittable temperature control device and polymerase chain reaction apparatus with the same |
| CN103173434A (en) * | 2011-12-23 | 2013-06-26 | 厦门万泰沧海生物技术有限公司 | Method and device for carrying out polymerase chain reaction under constant-temperature heat source |
| KR20130090587A (en) * | 2012-02-06 | 2013-08-14 | 김성용 | Potable isothermal nucleotide amplification device |
| CN203720089U (en) * | 2014-03-10 | 2014-07-16 | 任立松 | Fluorescence intensity analyzer for cellular loop-mediated isothermal amplification gene product |
| CN105917227A (en) * | 2013-11-12 | 2016-08-31 | 新加坡科技研究局 | Quantitative real-time and end-point colorimetric PCR device |
| CN109797097A (en) * | 2019-01-17 | 2019-05-24 | 浙江大学 | A kind of top illuminated nucleic acid isothermal amplification detection portable instrument |
| CN111902212A (en) * | 2018-02-07 | 2020-11-06 | 奥斯瓦道·克鲁兹基金会 | LAMP detection device |
-
2020
- 2020-12-24 CN CN202011552871.6A patent/CN112760214A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101109706A (en) * | 2007-08-31 | 2008-01-23 | 北京工业大学 | Centrifugal type PCR analyzer |
| CN101824486A (en) * | 2009-12-30 | 2010-09-08 | 华东医学生物技术研究所 | Method and device for rapidly detecting nucleic acid |
| KR20110137090A (en) * | 2010-06-16 | 2011-12-22 | 삼성테크윈 주식회사 | Light transmittable temperature control device and polymerase chain reaction apparatus with the same |
| CN103173434A (en) * | 2011-12-23 | 2013-06-26 | 厦门万泰沧海生物技术有限公司 | Method and device for carrying out polymerase chain reaction under constant-temperature heat source |
| KR20130090587A (en) * | 2012-02-06 | 2013-08-14 | 김성용 | Potable isothermal nucleotide amplification device |
| CN105917227A (en) * | 2013-11-12 | 2016-08-31 | 新加坡科技研究局 | Quantitative real-time and end-point colorimetric PCR device |
| CN203720089U (en) * | 2014-03-10 | 2014-07-16 | 任立松 | Fluorescence intensity analyzer for cellular loop-mediated isothermal amplification gene product |
| CN111902212A (en) * | 2018-02-07 | 2020-11-06 | 奥斯瓦道·克鲁兹基金会 | LAMP detection device |
| CN109797097A (en) * | 2019-01-17 | 2019-05-24 | 浙江大学 | A kind of top illuminated nucleic acid isothermal amplification detection portable instrument |
Non-Patent Citations (1)
| Title |
|---|
| 陈杉等: "乙型肝炎病毒重组酶聚合酶等温扩增闭管可视化检测方法及装置", 《医学研究生学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113981139A (en) * | 2021-09-27 | 2022-01-28 | 厦门健康工程与创新研究院 | Nucleic acid isothermal amplification detection reagent, kit, detection system and method |
| CN113981139B (en) * | 2021-09-27 | 2022-11-25 | 厦门健康工程与创新研究院 | Nucleic acid isothermal amplification detection reagent, kit, detection system and method |
| CN113755647A (en) * | 2021-10-15 | 2021-12-07 | 无锡市人民医院 | Novel coronavirus rapid detection system |
| CN113755647B (en) * | 2021-10-15 | 2023-08-22 | 无锡市人民医院 | Novel coronavirus rapid detection system |
| CN115216553A (en) * | 2022-05-26 | 2022-10-21 | 四川国际旅行卫生保健中心(成都海关口岸门诊部) | Deep learning-based digital detection system and detection method for plasmodium falciparum |
| CN115216553B (en) * | 2022-05-26 | 2023-11-24 | 四川国际旅行卫生保健中心(成都海关口岸门诊部) | Digital plasmodium falciparum detection system and detection method based on deep learning |
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