KR20110136558A - Cosmetic composition containing mycelial culture broth of mushrooms - Google Patents

Abstract

PURPOSE: A cosmetic composition containing mushroom mycelium culture liquid is provided to promote hyaluronan synthase-3 and to improve anti-wrinkling and moisturizing. CONSTITUTION: A cosmetic composition contains 0.01-30 wt% of mixture mycelium culture liquid of red koji, Cordyceps sinensis Sacc, and Sparassis crispa Wulf.:Fr. The mycelium culture liquid is prepared by culturing the mycelium of red koji, Cordyceps sinensis Sacc, and Sparassis crispa Wulf.:Fr. in a liquid medium containing 1-3 wt% of maltose, 0.5-2 wt% of peptone, 0.01-1 wt% of calcium chloride, 1-3 wt% of magnesium chloride, 0.1-0.5 wt% of ferric chloride, 0.1-0.5 wt% of citric acid, 0.005-0.2 wt% of manganese sulfate, 0.05-0.2 wt% of sulfate zinc, 1-5 wt% of monobasic potassium phosphate, 5-10 wt% of sodium monohydrogen phosphate, and 0.5-2 wt% of sodium sulfate.

Description

Cosmetic composition containing mycelial culture broth as an active ingredient {Cosmetic composition containing Mycelial Culture Broth of Mushrooms}

 The present invention relates to a cosmetic composition containing a mushroom mycelium culture medium, more specifically, mycelium cultured by inoculating the mycelium of hongguk, Cordyceps sinensis, zinnia mushroom in the medium as an active ingredient to improve skin wrinkles, moisturizing And it relates to a cosmetic composition having an activity such as promoting skin regeneration.

Humans are using cosmetics for cleanliness, beauty, and skin care regardless of their sex and age, and furthermore, cosmetics are used to change the skin to express beauty. Therefore, the function of cosmetics is gradually improving, and as the industry develops, cosmetics become more luxurious and diversified. As the cosmetics industry grows, it is trying to use safe materials that are not harmful to humans. In particular, natural materials including natural products and medicinal plants are being developed.

Humans have long used mushrooms not only in food but also in various fields such as antibiotics, vitamins and anticancer drugs. The β-glucan of mushroom-derived polysaccharides has been shown to promote the production of skin cell growth factors essential for skin burns and wound healing, and skin cell growth factors promote collagen biosynthesis to increase skin regeneration ability. In addition, the skin's moisture retention ability is known to play a role in maintaining the elasticity of the skin. Korean Patent Application No. 1999-4497 discloses a moisturizing cosmetic composition containing beta-1,6-branched beta-1,3-glucan, a polysaccharide (Schizophyllum commune), and Korean Patent Application No. 2003-73323 discloses Wrinkle improvement cosmetic composition containing a mushroom (Inonotus obliquus) extract is disclosed, the Korean Patent Application No. 2001-10171 discloses a whitening cosmetics containing the extract (Phellinus linteus). In addition, Korean Patent Application No. 2003-24042 discloses an external preparation composition for skin aging prevention and whitening and skin damage alleviation containing Hericium erinaceus fruiting body extract. The wrinkle improvement cosmetic composition containing is disclosed.

However, in the case of fruiting bodies, their composition changes according to the culture conditions and habitats. Therefore, in order to industrialize, it is necessary to develop a substance that can suppress wrinkles by cultivating liquid mycelium that can control the possible culture conditions, yield, and efficacy. Necessity is emerging. Therefore, the inventors of the present invention can be usefully used in cosmetic compositions in the case of mixing culture of red yeast, Cordyceps sinensis and zinnia mushroom mycelium in a specific medium while searching for industrializable materials using various mushrooms native to Korea in addition to commonly known mushrooms The present invention was completed by finding out.

It is an object of the present invention to provide a cosmetic composition containing a mushroom mycelium culture medium inoculated with a mycelium of red yeast rice, Cordyceps sinensis and zinnia mushroom in a medium.

In another aspect, the present invention is to provide a method for producing a mushroom mycelia culture solution contained in the cosmetic composition as an active ingredient.

According to the present invention to achieve the above object, there is provided a cosmetic composition containing 0.01 ~ 30% by weight of the mycelium culture solution of red yeast rice, Cordyceps sinensis and blossom mushroom relative to the total weight of the cosmetic composition. The mycelium culture medium is mixed mycelium of red yeast rice, Cordyceps sinensis and zinnia mushroom 1 to 3% by weight of maltose, 0.5 to 2% by weight peptone, 0.01 to 1% by weight of calcium chloride, 1-3% by weight of magnesium chloride, 0.1 to 0.5% by weight of iron chloride , 0.1 to 0.5% by weight of citric acid, 0.005 to 0.2% by weight of manganese sulfate, 0.05 to 0.2% by weight of zinc sulfate, 1 to 5% by weight of potassium dihydrogen phosphate, 5 to 10% by weight of sodium dihydrogen phosphate, 0.5 to 2% of sodium sulfate It is characterized in that obtained by culturing in a liquid medium containing weight%.

The mycelium of the hongguk, Cordyceps sinensis and blossom mushroom is added in a weight ratio of 1: 1 to 2: 1 to 2 is to be cultured mixed, preferably it is mixed and cultured in a ratio of 1: 1: 1. The total mycelium is preferably inoculated in a liquid medium at 20 to 40% (v / v) and mixed and cultured.

The cosmetic composition is characterized by promoting the biosynthesis of collagen to improve wrinkles, promote the expression of hyaluronan synthase-3 (hyaluronan synthase-3) in the skin to increase the moisturizing power and regenerates the skin.

The cosmetic composition containing the mushroom mycelium culture medium skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, essence, pack, soap, It can be applied in any one formulation selected from the group consisting of shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder and eye shadow.

In order to achieve the above object, according to the present invention, 1 to 3% by weight of red yeast, Cordyceps sinensis and blossom mushroom mycelium 1 to 3% by weight, 0.5 to 2% by weight peptone, 0.01 to 1 calcium chloride % By weight, 1 to 3% by weight of magnesium chloride, 0.1 to 0.5% by weight of iron chloride, 0.1 to 0.5% by weight of citric acid, 0.005 to 0.2% by weight of manganese sulfate, 0.05 to 0.2% by weight of zinc sulfate, 1 to 5 of potassium dihydrogen phosphate Inoculating a liquid medium containing weight%, 5 to 10 weight% sodium dihydrogen phosphate, and 0.5 to 2 weight% sodium sulfate;

incubation for 7-15 days at pH 5.7-6.0, temperature 25-30 ° C .; And

After homogenizing the culture solution is provided a method for producing a mushroom mycelium culture medium comprising the step of centrifugation for 10 to 15 minutes at 3000rpm.

Cosmetic composition of the present invention containing a mycelium culture of red yeast rice, Cordyceps sinensis, zinnia mushroom promotes collagen biosynthesis, improves skin wrinkles, and produces hyaluronan which proceeds with biosynthesis of hyaluronan (HA), which is well known to retain moisture By promoting the expression of hyaluronan synthase-3 (hyaluronan synthase-3) it has the effect of promoting skin moisturizing and skin regeneration.

1A and 1B are graphs showing an optimal carbon source and an optimal nitrogen source of a mushroom mycelium culture medium according to an embodiment of the present invention.
Figure 2 is a graph showing the optimum culture temperature of the mushroom mycelium culture medium according to an embodiment of the present invention.
Figure 3 is a graph showing the optimum mixing ratio of the mushroom mycelium culture medium according to an embodiment of the present invention.
Figure 4 is a graph showing the optimum amount of inoculation mushroom mycelium culture medium according to an embodiment of the present invention.
5 is a graph showing the optimum culture pH of the mushroom mycelium culture medium according to an embodiment of the present invention.
Figure 6 is a graph showing the optimum incubation time of the mushroom mycelium culture medium according to an embodiment of the present invention.
Figure 7 is a photograph showing the cell regeneration effect of the mushroom mycelium culture medium according to an embodiment of the present invention.

The present invention relates to cosmetics using the collagen production promoting and hyaluronan synthase-3 expression promoting effect of the red yeast, Cordyceps sinensis and the mushroom mycelium culture.

Fungi are large groups that contain eukaryotic organisms such as yeasts, fungi, and mushrooms. They are classified into plants, animals, and bacteria, and most of the spores germinate to form mycelium, grow to fruiting bodies, and form fruiting bodies. The process of growing spores is repeated. Fungi contain biopolymer chitin as well as glucans (β-1,3-glucan) in their cell walls. Plants contain cellulose in their cell walls, whereas fungi contain chitin, which is one of the main differences. Can be. Among the biologically similar mushrooms, mushrooms are among the most similar species. Mushrooms grow according to various growth conditions such as nutrition, temperature, humidity, light, oxygen, and acidity, and depending on the physiological function, medium, host surface, organic matter, moderate humidity and temperature. If the environmental conditions are good, a tissue is formed. Mushrooms have an active ingredient in the cell wall or in the cell membrane, and in some cases they form fruiting bodies and secrete the active ingredient outside the cell. Fungi grow in the form of tubular filaments, called hyphae, and these hyphae masses are called mycelium. In functional terms, the mycelium can be likened to the nutrient organs and the fruiting bodies to the reproductive organs, and what is called a mushroom is the fruiting body and the mycelium cannot be observed with the naked eye. In terms of nutrition, mycelium is a powerful active substance that accumulates nutrients and active substances for growing fruiting bodies as it holds deeply the energy for growing mushrooms. Therefore, the core of the mushroom's energy is in the spores and mycelium.

Cordyceps militaris has antioxidant and hypolipidemic effects and contains skin elasticity and moisturizing ingredients. Monascus pilosus inhibits anti-inflammatory effect and cholesterol, and Sparassis crispa has anticancer activity. It is known to have a high content of beta glucan and has an excellent effect on boosting immune function.

The present invention is characterized in that the culture medium obtained by mixing and cultivating red yeast, Cordyceps sinensis and zinnia mushroom mycelium at a specific ratio in a specific composition, exhibits excellent skin wrinkle improvement, moisturizing and skin regeneration by synergy. It is done.

In the present invention, the mycelium of red yeast rice, Cordyceps sinensis and blossom mushroom showed an excellent effect when mixed culture in a ratio of 1: 1 to 2: 1 to 2, as shown in Figure 3, 1: 1: 1 The best effect is obtained when mixed cultures by weight ratio.

The mushroom mycelium culture solution of the present invention has a mechanism of action to improve skin wrinkles by promoting the biosynthesis of collagen in the skin. In addition, by promoting the expression of hyaluronan synthase-3 (hyaluronan synthase-3) has a mechanism of action to increase the skin moisturizing power.

Mycelium culture of red yeast rice, Cordyceps sinensis and blossom mushroom of the present invention can be prepared as follows. First, the mycelium of hongguk, Cordyceps sinensis, zinnia mushroom is inoculated with the spawn of the mushroom mycelia culture medium. Solid culture for bacterial growth of the mushroom mycelium is 5 ~ 10 days, preferably at a temperature of 25 ~ 32 ℃, preferably 27 ~ 30 ℃ and pH 5.0 ~ 6.0, preferably pH 5.7 ~ 6.0 in PDA medium It is recommended to incubate for 7-10 days.

Using the obtained mushroom mycelium as the seed, liquid culture is carried out as follows to obtain a culture solution. The seed is incubated by inoculating 20 to 40% (v / v) in a liquid medium having the following composition. The effect on the inoculation amount is shown in FIG. 4. As shown in Figure 4 showed the best effect when inoculated at 20% (v / v).

Liquid culture of the mushroom mycelium is 1 to 3% by weight maltose, 0.5 to 2% by weight peptone, 0.01 to 1% by weight calcium chloride, 1-3% by weight magnesium chloride, 0.1 to 0.5% by weight iron chloride, 0.1 to 0.5 citric acid In a medium containing% by weight, 0.005 to 0.2% by weight manganese sulfate, 0.05 to 0.2% by weight zinc sulfate, 1 to 5% by weight potassium dihydrogen phosphate, 5 to 10% by weight sodium dihydrogen phosphate, 0.5 to 2% by weight sodium sulfate It is recommended to incubate for 7-15 days under the condition of pH 5.7 ~ 6.0 and 25 ~ 30 ℃.

After homogenizing the culture solution, the culture solution obtained by centrifugation at 3000 rpm for 10 to 15 minutes is used as a cosmetic. The mushroom mycelium broth may be concentrated or purified.

[Example]

Hereinafter, the present invention will be described in detail by way of the following examples and test examples, but the present invention is not limited to these examples.

Preparation Examples 1-4: Preparation of mycelia culture medium

Monascus pilosus ), Cordyceps militaris ) and Sparassis crispa were used as strains, and passaged on PDA (Potato Dextrose Agar, DIFCO, USA) flat medium, and reagents were used by DIFCO's special reagent.

In order to select the optimal medium for cosmetic raw materials, the red yeast ( Monascus pilosus ), Cordyceps militaris ), Sparassis crispa ) and mixed mycelium of the same weight ratio thereof were incubated for 8 days at 30 ° C. and pH 6.0 with different carbon and nitrogen sources based on CMM medium as shown in Table 1 and Table 2 below. After incubation, centrifugation at 3000 rpm for 10 to 15 minutes was used for the experiment.

Furtherance unit Carbon source
(Maltose / glucose / starch / lactose / fluctose) 10 g / L KNO ₃ 2 g / L Trace element ^a) 40 ml / L Salt solution ^b) 25 ml / L Thiamine solution (0.1mg / ml) 1 ml / L ^a) 2.73 g / L CaCl ₂
MgCl ₂ -6H ₂ O 10.25 g / L
_{FeCl 3 -6H 2 O 1.33 g /} L
Citric acid 1.33 g / L
MnSO ₄ 1.1 g / L
ZnSO ₄ 1.0 g / L

^b) Ammonium tartrate 20 g / L
KH ₂ PO ₄ 40 g / L
Na ₂ HPO ₄ 90 g / L
Na ₂ SO ₄ 11.2 g / L

Furtherance unit Maltose 10 g / L Nitrogen source
(Peptone / yeast / KNO ₃ / malt extract) 2 g / L Trace element ^a) 40 ml / L Salt solution ^b) 25 ml / L Thiamine solution (0.1mg / ml) 1 ml / L ^{a), b)} as ^{shown in} Table 1.

Mycelium density was visually observed, and the collagen biosynthesis test and the expression of hyaluronan synthase-3 were tested according to the test method described later. After incubating with different carbon and nitrogen sources, the results of testing the collagen biosynthesis and the expression of hyaluronan synthase-3 are shown in FIGS. 1A and 1B.

As confirmed in FIGS. 1A and 1B, Monascus pilosus ), Cordyceps militaris ), Sparassis crispa ) When the mycelium is cultured in a medium using maltose as a carbon source and peptone as a nitrogen source, the most excellent collagen biosynthesis and hyaluronan synthase-3 The expression result was shown, and even in the case of the mixed strain of the mushroom mycelium, it showed the best effect in the medium containing maltose and peptone.

Example 1 Preparation of Mushroom Mycelium Mixed Culture Solution

Optimal medium established through the preparation example using a cordyceps militaris , Cordyceps militaris , Monascus pilosus , Sparassis crispa mixed strains of the same weight ratio, that is, a medium having a composition as shown in Table 3 below (modified CMM) ) Was cultured by inoculating the mixed mushroom mycelium in an optimal medium at a ratio of 20% (v / v).

In order to determine the optimum growth temperature conditions were incubated at 15, 20, 25, 30 and 35 ℃, and the other culture conditions were the same as the culture conditions of the preparation example.

Furtherance unit Maltose 10 g / L Eptone 2 g / L Trace element ^a) 40 ml / L Salt solution ^b) 25 ml / L Thiamine solution (0.1mg / ml) 1 ml / L ^{a), b)} as ^{shown in} Table 1.

Monascus pilosus ), Cordyceps militaris ) and Sparassis Each culture solution obtained by culturing crispa ) strain was used as a comparative example, and collagen biosynthesis and hyaluronan synthase-3 expression test results according to temperature are shown in FIG. 2.

As a result, as shown in FIG. 2, collagen biosynthesis and HAS-3 expression amount were excellent by mushroom mycelium culture medium cultured at 30 ° C., and the mixed strain culture medium showed more excellent effect than the mushroom mycelium culture medium. .

Example 2 Preparation of Mushroom Mycelium Mixed Culture Solution

Cordyceps militaris , red yeast ( Monascus pilosus ) and zinnia mushroom ( Sparrassis ) in the same weight ratio crispa ) mixed strains were cultured at different pHs (pH5, pH5.5, pH6, pH6.5, pH7) in the optimal growth medium of Example 1. Other culture conditions were the same as those of Example 1 above.

Monascus pilosus ), Cordyceps militaris ) and Sparassis crispa ) cultures obtained by culturing the strain as a comparative example, collagen biosynthesis by pH, the expression test results of HAS-3 are shown in FIG.

As a result, as shown in FIG. 5, collagen biosynthesis and HAS-3 expression amount by mushroom mycelium culture medium cultured at pH 5.5 were the best, and the mixed strain culture solution of Example 2 showed the best effect.

Example 3: Preparation of mushroom mycelium mixed culture solution

Cordyceps militaris of the same weight ratio, Cordyceps militaris , Monascus pilosus and Sparassis crispa mixed strain in different culture times in the optimum growth medium of Example 1 (5 days, 10 days, 15 days, 20 Work). Other culture conditions were the same as those of Example 1 above.

Monascus pilosus ), Cordyceps militaris ) and Sparassis Each culture solution obtained by culturing crispa ) strain was used as a comparative example, and collagen biosynthesis and hyaluronan synthase-3 expression test results according to culture time are shown in FIG. 6.

As a result, as shown in Figure 6 collagen biosynthesis and HAS-3 expression by the mushroom mycelium culture medium cultured for 10 days was the best, the mixed strain culture solution of Example 3 showed the best effect.

Test Example 1: Collagen Biosynthesis Promotion Effect Test

Keratinocytes were inoculated in DMEM medium with 10% FBS at a cell concentration of 1 × 10 ⁶ and incubated in 37 ° C., 5% CO ₂ incubator for 24 hours. Cultivation of red yeast rice, Cordyceps sinensis, blossom mushroom mycelium and their same weight mixed strains, which were incubated for 24 hours and cultivated under optimum growth conditions (30 ° C., pH 5.5, 10 days of incubation period) established in Examples 1 to 3, respectively. One culture solution was diluted in DMEM medium so that the final concentration was 0.5%, and the mixture was incubated under the same conditions for 18 hours. After that, cells were recovered using Trizol reagent (invitrogen, USA), mRNA was extracted, and cDNA was synthesized through a series of processes. Procollagen type I gene region was amplified from the synthesized cDNA to confirm gene expression by electrophoresis. Gene amplification was performed using thermal cycler (GenePro, Hangzhou bioer tech., CHINA) using 10x taq polymerase buffer, 10 mM dNTP, 10 pmol primer (F: AGC CAG CAG ATC GAG AAC AT, R: TCT TGT CCT TGG GGT TCT TG), mixed with taq polymerase and adjusted to 50 uL with distilled water 95 ° 5 min 1 cycle / 95 ° 1 min / 51 ° 2 minutes / 72 degrees 1 minute 28 cycles amplification, was carried out under the conditions for 5 minutes at 72 degrees. The expression rate of procollagen produced was calculated according to the following equation, and control cells were used as untreated samples.

Procollagen Expression (%) = B / A × 100 (%)

A: amount of procollagen expression in the control group

B: amount of procollagen expression in sample treated cells

The test results were obtained as shown in Table 4 below.

Sample concentration
(Μg / ml) procollagen expression rate (%) Untreated Red yeast rice Cordyceps sinensis Blossom Mushroom Red chrysanthemum + Cordyceps sinensis + Mushroom 500 15 40 64 71 86

As confirmed in the results of Table 4, it was found that the collagen biosynthesis-promoting effect related to the skin wrinkles was higher when the culture medium of the mixed strains were applied than the hongguk, Cordyceps sinensis, blossom mushroom mycelium culture alone.

Test Example 2: Expression promoting effect test of Hyaluronan synthase-3

Keratinocytes were inoculated at a concentration of 1 × 10 ⁶ in DMDM medium containing 10% FBS and incubated for 24 hours in a 37 ° C., 5% CO ₂ incubator. Cultivation of red yeast rice, Cordyceps sinensis, blossom mushroom mycelium and their same weight mixed strains, which were incubated for 24 hours and cultivated under optimum growth conditions (30 ° C., pH 5.5, 10 days of incubation period) established in Examples 1 to 3, respectively. One culture solution was diluted in DMEM medium so that the final concentration was 0.5%, and the mixture was incubated under the same conditions for 18 hours. MRNA was extracted in the same manner as in Test Example 1, and cDNA was synthesized through a series of processes. The amount of gene expression was confirmed by electrophoresis by amplifying the hyaluronan synthase-3 gene region from the synthesized cDNA, and gene amplification is the same as in Test Example 1. 10x taq polymerase buffer, 10 mM dNTP, 10 pmol primer (F: GAG GAC TGG TAC CAT CAG AA, R: GCC AGA TTT GTT GAT GGT AGC), taq polymerase was mixed and adjusted to 50 uL by distilled water 94 Minute 1cycle / 94 degrees 30 seconds / 50 degrees 30 seconds / 72 degrees 1 minute 28 cycles amplification, was carried out under the conditions of reacting for 5 minutes at 72 degrees. The expression rate of the generated hyaluronan synthase-3 was calculated according to the following equation, and the control group was used as a sample untreated cells. The results are shown in Table 5 below.

HAS-3 expression rate (%) = B / A × 100 (%)

A: hyaluronan synthase-3 expression in the control group

B: Hyaluronan synthase-3 expression level in sample treated cells

Sample concentration
(Μg / ml) hyaluronan synthase-3 expression rate (%) Untreated Red yeast rice Cordyceps sinensis Blossom Mushroom Red chrysanthemum + Cordyceps sinensis + Mushroom 500 13 69 47 66 88

As confirmed in the results of Table 5, the hyaluronan synthase-3 expression promoting effect was related to the skin moisturizing effect when the culture medium of the mixed strains was applied than when the red yeast, Cordyceps sinensis, and the mycelium mushroom mycelium cultures were applied alone.

Test Example 3 Skin Regeneration Effect Test

Keratinocytes were inoculated at a concentration of 1 × 10 ⁶ in DMDM medium containing 10% FBS and incubated for 12 hours at 37 ° C. in a 5% CO ₂ incubator. Scratch the cells by incubation for 12 hours, and mix the same weight of red yeast, Cordyceps sinensis, and mushroom mycelium cultured under optimal growth conditions (30 ℃, pH 5.5, 10 days incubation period) established in Examples 1 to 3. Dilution solution prepared by diluting the culture medium in which the strain was cultured to a final concentration of 0.5% DMEM medium was added to check the cell regeneration rate at 4 hour intervals. As a control, cells that were not treated with the sample were used, and the results are shown in FIG. 7 and Table 6 below.

sample Skin regeneration rate (%) Hongkuk + Cordyceps sinensis + Mixed mushroom culture 82 Control 30

As confirmed in the results of Table 6, when the red yeast, Cordyceps sinensis, blossom mushroom mycelium mixed culture solution was applied, it showed an excellent cell regeneration promoting effect.

Example 4 Preparation of Nutritional Cream

Contains culture medium in which the same weight mixed strains of red yeast rice, Cordyceps sinensis, and blossom mushroom mycelium were cultivated under optimum growth conditions (30 ° C., pH 5.5, 10 days incubation period) established in Examples 1 to 3 of the present invention. Nutritional creams were prepared according to the composition of Table 7 for the clinical test on the skin moisturizing effect and skin safety of the cosmetics.

A nutritious cream containing no mushroom mycelium culture medium of the present invention was prepared as Comparative Example 1.

ingredient weight% Example 4 Comparative Example 1 Mushroom mycelium broth 5.00 - glycerin 2.00 2.00 Butylene Glycol 5.00 7.50 Arakidil Alcohol / Behenyl Alcohol /
Arachidil Glucoside 2.00 2.00 Caprylic / capric triglyceride 6.00 6.00 Microcrystalline Wax 1.00 1.00 Cetearyl Alcohol 3.00 3.00 Mineral oil 4.00 4.00 Carbomer 0.15 0.15 Triethanolamine 0.15 0.15 Propylparaben 0.05 0.05 Butyl Paraben 0.05 0.05 Methylparaben 0.20 0.20 incense Quantity Quantity Purified water to 100 to 100

Test Example 4 Skin Moisturizing Enhancement Effect

The skin moisturizing enhancement effect of the cosmetic composition of Example 4 containing the red yeast, Cordyceps sinensis, zinnia mushroom mycelium culture medium of the present invention was measured in 20 healthy women aged 25 to 50 years. First, it was divided into two groups, and the formulation of Example 4 in Group A and Comparative Example 1 in Group B was applied to the face twice a day (morning and evening) for 3 months. Three months later, the degree of skin moisturizing was evaluated through a questionnaire and image analysis of the moisturizing. The subject's questionnaire was judged as four stages of no improvement, slight improvement, moderate improvement, and significant improvement compared to before use, and the results are shown in Table 8 below.

sample No improvement Minor improvements Moderate improvement A significant improvement Example 4 One 2 4 3 Comparative Example 1 4 4 One One

In the evaluation of moisturizing by image analysis, the lower part of the eye was measured by using ARAMO-TS, and the moisture value was measured by using the capacitance of the skin surface. The same site was measured. Skin moisture measurement results by image analysis is shown in Table 9 below as a reduction rate for the moisture value compared to before use.

sample Skin Moisturizing Growth Rate (%) Example 4 78 Comparative Example 1 20

From Tables 8 and 9, it can be seen that the skin moisturizing enhancement effect of Example 4 of the present invention is very excellent.

Test Example 5: Formulation Stability Test

The stability test for the cosmetics containing the red yeast rice, Cordyceps sinensis, and blossom mushroom mycelium culture of the present invention was carried out by the following method. In order to test the stability of the cosmetic Example 4 and Comparative Example 1 in an opaque glass container kept in a constant temperature maintained at 45 ℃ constant storage for 12 weeks and also opaque in a fully shaded refrigerator kept constant at 4 ℃ After storing for 12 weeks in a glass container, discoloration and odor, and the degree of separation is measured and the results are shown in Table 10 below. At this time, the product discoloration, odor and separation degree was classified into six grades and evaluated.

0: No change 1: Very little separation (color change, odor)

2: Slightly separated (discolored, decolored) 3: Slightly separated (discolored, decolored)

4: Severe separation (discoloration, odor) 5: Extremely severe separation (discoloration, odor)

division Formulation Stability Example 4 Comparative Example 1 45 ℃ 4 ℃ 45 ℃ 4 ℃ discoloration 0 0 0 0 Deodorant 0 0 0 0 detach 0 0 0 0

As can be seen from Table 10, in the case of Example 4 and Comparative Example 1, it was stable at almost 45 ° C. and 4 ° C. without discoloration, odor, and separation.

Claims (7)

A cosmetic composition containing 0.01 to 30% by weight of the mixed mycelium culture medium of red yeast rice, Cordyceps sinensis and flower mushroom, based on the total weight of the cosmetic composition. According to claim 1, wherein the mycelium culture medium of the mycelium of red yeast, Cordyceps sinensis and zinnia mushroom maltose 1 to 3%, peptone 0.5 to 2% by weight, calcium chloride 0.01 to 1% by weight, magnesium chloride 1 to 3% by weight, iron chloride 0.1 to 0.5% by weight, citric acid 0.1 to 0.5% by weight, manganese sulfate 0.005 to 0.2% by weight, zinc sulfate 0.05 to 0.2% by weight, potassium dihydrogen phosphate 1 to 5% by weight, sodium dihydrogen phosphate 5 to 10% by weight , Cosmetic composition characterized in that obtained by culturing in a liquid medium containing 0.5 to 2% by weight of sodium sulfate. The cosmetic composition according to claim 1 or 2, wherein the mycelium of the red yeast rice, Cordyceps sinensis and the blossom mushroom is added and mixed in a weight ratio of 1: 1 to 2: 1 to 2. The cosmetic composition according to claim 1, wherein the mycelia culture medium promotes biosynthesis of collagen and promotes expression of hyaluronan synthase-3. According to claim 1, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, pack, soap, Cosmetic composition, characterized in that applied to any one formulation selected from the group consisting of shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder and eye shadow. 1 to 3% by weight of red yeast, Cordyceps sinensis and flower mushroom mycelium in a ratio of 1: 1 to 2: 1 to 2 by weight, 0.5 to 2% by weight of peptone, 0.01 to 1% by weight of calcium chloride, 1-3% by weight of magnesium chloride, iron chloride 0.1 to 0.5% by weight, citric acid 0.1 to 0.5% by weight, manganese sulfate 0.005 to 0.2% by weight, zinc sulfate 0.05 to 0.2% by weight, potassium dihydrogen phosphate 1 to 5% by weight, sodium dihydrogen phosphate 5 to 10% by weight Inoculating a liquid medium containing 0.5 to 2% by weight of sodium sulfate;
incubation for 7-15 days at pH 5.7-6.0, temperature 25-30 ° C .; And
Method for producing a mushroom mycelium culture medium comprising the step of centrifugation for 10 to 15 minutes at 3000 rpm after homogenizing the culture solution. The method according to claim 6, wherein the mushroom mycelium is inoculated in a liquid medium at 20 to 40% (v / v) to incubate.

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