KR20200108748A - The cultivation method of sang hwang mushroom mycelium with high content of beta-glucan - Google Patents

Abstract

The present invention relates to a cultivation method of Phellinus linteus mycelium with high beta-glucan content. The cultivation method of Phellinus linteus mycelium with high beta-glucan content of the present invention comprises: a first process for producing mulberry leaf powder by drying and pulverizing mulberry leaves; a second process of inoculating the Phellinus linteus mycelium into a medium and culturing the same after preparing a solid medium containing the mulberry leaf powder produced in the first process to produce a cultured product; a third process of inoculating the cured product of the second process in the medium and culturing the same after preparing a liquid medium containing the mulberry leaf powder prepared in the first process to produce a cultured liquid; a fourth process of preparing grains and mixing the mulberry leaf powder prepared in the first process and the grains in a weight ratio of 1 to 3:100 to produce a grain medium; and a fifth process of inoculating 3 to 10 mL of a culture solution of the third process per 100 g of the grain medium into the grain medium, and culturing the same to culture the Phellinus linteus mycelium. According to the present invention, a cultivation method is capable of mass-producing Phellinus linteus mycelium using mulberry leaves in a short time, and the Phellinus linteus mycelium has high beta-glucan content through optimal cultivation conditions.

Description

Cultivation method of P. mushroom mycelium with maximized beta-glucan content{THE CULTIVATION METHOD OF SANG HWANG MUSHROOM MYCELIUM WITH HIGH CONTENT OF BETA-GLUCAN}

Cultivation method of Pseudomonas mushroom mycelium with maximized beta-glucan content

The present invention relates to a method for cultivating a mushroom mycelium with maximizing beta-glucan content.

Beta-glucan is a term that refers to a polysaccharide in which glucose is polymerized around beta (β)-1,3 chemical bonds, and is present in the epidermis of cereals, mycelium and fruiting bodies of mushrooms, cell walls of yeast, and extracellular polysaccharides of bacteria. It is known, and it is known to have the effect of activating immune cells, anti-cancer activity, reducing body fat and lowering cholesterol.

Mushrooms containing these beta-glucans include Pseudomonas mushrooms, which naturally grow on old mulberry trees, so it is not only difficult to easily obtain Pseudomonas mushroom strains, and it is difficult to distinguish whether the obtained Pseudomonas mushroom strains are Pseudomonas mushrooms. There is a more difficult problem for ordinary people to produce.

In addition, when cultivating Pseudomonas mushrooms, sawdust medium, malt extract medium, peptone medium, etc. are commonly used as culture medium, but mass production is difficult because they are not well cultured in such a medium. Therefore, in order to solve this problem, various studies are being conducted as follows.

In Korean Patent Application Publication No. 10-206-0008341 (A method for culturing P. mushroom mycelium in a grain medium containing coated green tea leaves), green tea coated with a certain amount of yeast extract on grains made of starchy substances such as brown rice, barley rice, and wheat rice. By adding leaves, fiber and porosity are increased, and through this, the cultivation period of P. mushroom mycelium is shortened, the color of yellow to yellow peculiar to Pseudomonas mushrooms appears darker, and beta, an immunity enhancing substance unique to Psoriasis mushrooms. A method for producing functional Pseudomonas mushroom mycelium, which emphasizes the differentiation of nutritionally superior quality to increase glucan content, has been disclosed.

However, the above invention causes the hassle of coating green tea leaves, and although it is said that the content of beta-glucan is increased, the exact content is not described, and it is described that it is increased by only 15 times than that of the native mushroom. It cannot be seen that the amount has been improved.

In Korean Patent Application Publication No. 10-2004-0024757 (a method for rapid cultivation of Pseudomonas mushroom mycelium using grains), the medium composition is adjusted by mixing an accelerator such as glucose into grains so that Pseudomonas mushroom mycelium can grow smoothly in cereals. A method for rapid cultivation of Pseudomonas mushroom mycelium, which enables rapid cultivation, has been disclosed.

In Korean Registered Patent Publication No. 10-0393254 (Situation mushroom attribute cultivation medium and Pseudomonas mushroom mycelium attribute cultivation method using it),

A method of shortening the cultivation period of Pseudomonas mushroom mycelium by adding oak juice extracted from oak as a culture medium component of Pseudomonas mushrooms and allowing mass production within a short time has been disclosed.

However, the above invention was invented for the purpose of shortening the cultivation period of Pseudomonas mushroom mycelium and mass production within a short period of time, and it is not mentioned how much the content of beta-glucan contained in Pseudomonas mushrooms is enhanced. You can see the difference.

In order to solve the above problems, the present invention has an object to provide both methods capable of mass-producing P. mushroom mycelium in a short time using mulberry leaves.

In addition, there is also an object to provide a mushroom mycelium with a high beta-glucan content through optimal culture conditions.

The present invention relates to a method of cultivating a mushroom mycelium with a high beta-glucan content.

The cultivation method of the mushroom mycelium with a high beta-glucan content of the present invention comprises a first step of preparing mulberry leaf powder by drying and pulverizing mulberry leaves, and preparing a solid medium containing mulberry leaf powder prepared in the first step. Then, after inoculation of P. mushroom mycelium in the medium, the second step of producing a culture by culturing, and after preparing a liquid medium containing mulberry leaf powder prepared in the first step, the culture of the second step in the medium The third step of inoculating and then culturing to prepare a culture solution, the fourth step of preparing a grain medium by mixing the mulberry leaf powder and grains prepared in the first step after preparing grains in a ratio of 1 to 3: 100 by weight, grain medium It consists of a fifth step in which 3 to 10 ml of the culture solution of the third step per 100 g is inoculated into the grain medium and then cultured to cultivate the P. mushroom mycelium. Phellinus linteus, which is the main material of the present invention, is a family of basidiomycete Minorum Mushrooms, and is a kind of Pseudomonas mushroom. Psoriasis mushrooms have excellent anticancer effects and are known to have excellent efficacy in enhancing immunity. The mushroom grows on the trunks of mulberry, oak, oak, and pine, and among them, the only mushroom that grows on the mountain mulberry by genetic analysis is said to be the only mushroom.

Another main material, mulberry leaves, is the leaves of Morus, which are ovate round or long elliptical round, split into 3 to 5 pieces, 10 cm long, with dull serrations at the edge, and are usually used to grow silkworms. Has become.

The present invention prepares a solid medium containing mulberry leaf powder by preparing mulberry leaves in a powder state by using the characteristics of Pseudomonas mushrooms growing on a mulberry tree as described above, and then inoculating Pseudomonas mushroom mycelium in this medium to obtain pure Psoriasis mushroom mycelium. Acquired, and when cultured by inoculating the so-grown Pseudomonas mushroom mycelium in a liquid medium containing mulberry leaf powder again, the Pseudomonas mushroom mycelium could be easily cultured in a short period of time.

At this time, as a result of several studies to find out the optimal culture conditions to obtain the maximum content of beta-glucan, a solid medium containing 0.5 to 3 g of mulberry leaf powder per 100 ml of the solid medium was prepared. Then, after inoculation of P. mushroom mycelium, culture was prepared at 20 to 32°C for 5 to 10 days, and a liquid medium containing 0.5 to 3 g of mulberry leaf powder per 100 ml of liquid medium was prepared. , After inoculating the culture, cultured at 20 to 32°C for 3 to 5 days to prepare a culture solution, and then mixed mulberry leaf powder and cereals in a weight ratio of 1 to 3: 100 to prepare a cereal medium, and then a cereal medium Per 100 g, 3 to 10 ml of the above-mentioned Pseudomonas mushroom mycelium culture solution is inoculated into cereals medium, and then cultured for 10 to 25 days at 100 to 1000 Lux and 20 to 32°C to cultivate Pseudomonas mushroom mycelium. As the porosity is increased, Pseudomonas mushroom mycelium can easily penetrate into cereals, so that Pseudomonas mushrooms mycelium with high beta-glucan content could be cultured.

According to the present invention, there is provided a cultivation method capable of mass-producing P. mushroom mycelium in a short time by using mulberry leaves.

In addition, through the optimal culture conditions, the beta-glucan content is provided with a high content of mushroom mycelium.

Figure 1 is a cultivation method of the situation mushroom mycelium with a high beta-glucan content.

The present invention describes the cultivation method of the mushroom mycelium with a high beta-glucan content as follows.

1. Cultivation method of Psoriasis mushroom mycelium with high beta-glucan content

1) Manufacture of mulberry leaf powder

After drying the mulberry leaves, they are pulverized to prepare mulberry leaf powder.

When pulverizing mulberry leaves, it is preferable to pulverize the mulberry leaf powder so that the particle size is 0.1 to 10 mm.

2) Culture preparation

After preparing a solid-state medium containing the mulberry leaf powder, inoculated with Pseudomonas mushroom mycelium in the medium, and then cultured to prepare a culture.

At this time, when 4 g or more of mulberry leaf powder is added per 100 ml of solid medium, the diameter of the cultivated Pseudomonas mushroom mycelium does not show a significant difference from when 3 g of mulberry leaf powder is added. It is preferable to prepare a solid medium containing 0.5 to 3 g of mulberry leaf powder per 100 ml of the medium, and then inoculate the mushroom mycelium, and then incubate at 20 to 32° C. for 5 to 10 days to prepare a culture.

In addition, it is preferable to prepare a solid medium containing mulberry leaf powder having a particle size of 0.1 to 1.0 mm.

3) Preparation of culture solution

After preparing a liquid medium containing the mulberry leaf powder, the culture medium is inoculated and cultured to prepare a culture medium.

At this time, when more than 4 g of mulberry leaf powder is added per 100 ml of the liquid medium, the yield of the cultivated Pseudomonas mushroom mycelium does not show a significant difference from that when 3 g of the mulberry leaf powder is added. After preparing a liquid medium containing 0.5 to 3 g of mulberry leaf powder per 100 ml of the medium, inoculating the culture of the second step, it is preferable to prepare a culture solution by inoculating the culture at 20 to 32°C for 3 to 5 days. Do.

In addition, it is preferable to prepare a liquid medium containing mulberry leaf powder having a particle size of 0.1 to 1.0 mm.

4) Manufacture of grain medium

After preparing cereals, mulberry leaf powder and cereals are mixed in a weight ratio of 1 to 3: 100 to prepare a cereal medium. At this time, as grains, one selected from brown rice, rice, corn, barley, wheat, adlay, sorghum, and soybeans is used. In addition, it is preferable to prepare a grain medium using mulberry leaf powder having a particle size of 1.0 to 10 mm. This, by increasing the porosity of the grain medium, has the effect of reducing the cultivation period by smooth oxygen supply.

5) cultivation of P. mushroom mycelium

3 to 10 ml of the culture solution per 100 g of the grain medium is inoculated into the grain medium, and then cultured to culture the P. mushroom mycelium.

At this time, it is preferable to incubate for 10 to 25 days at 100 to 1000 Lux, 20 to 32 °C. Hereinafter, the present invention will be described in detail through examples and experimental examples, but these do not limit the scope of the present invention.

<Example 1> Beta-glucan content of the present invention is high cultivation of mushroom mycelia 1

After preparing mulberry leaves, drying them, and then pulverizing the dried mulberry leaves with a grinder to prepare mulberry leaf powder.

YMA medium (yeast extract 0.3 g, malt extract 0.3 g, glucose 1 g, peptone 0.5 g, Agar 1.8 g, distill water 96.1 mL, pH 5.0) was added with 3 g mulberry leaf powder with a particle size of 0.2 mm. After sterilization at 121° C. for 20 minutes, a solid medium was prepared.

Phellinus linteus and Phellinus linteus and Phellinus linteus were prepared by direct separation from wild Pseudomonas mushroom mycelium (as a result of analyzing the ITS-5.8S rDNA sequence of classification number BPL by requesting the Korea Microorganism Conservation Center), and then the solid state A culture was prepared by inoculating P. mushroom mycelium in the medium of and incubating at 26° C. for 10 days.

YMB medium (yeast extract 0.3 g, malt extract 0.3 g, glucose 1 g, peptone 0.5 g, distill water 97.9 mL, pH 5.0) to 100 mL of liquid medium containing 3 g of mulberry leaf powder with a particle size of 0.2 mm Was prepared after sterilizing at 121° C. for 20 minutes.

The culture was inoculated into the liquid medium and cultured at 200 rpm, 1.0 vvm, and 26° C. for 5 days to prepare a culture solution.

After preparing brown rice, 300 g of brown rice was added to distilled water, immersed at 35° C. for 24 hours, and then taken out to remove moisture, and mixed with 3 g of mulberry leaf powder having a particle size of 10 mm to prepare a grain medium.

After inoculating 20 ml of the culture medium into 300 g of the prepared grain medium, incubate at 800 Lux and 26 ℃ for 25 days to

The mycelium was cultured.

<Example 2> The beta-glucan content of the present invention is high cultivation of mushroom mycelia 2

Cultured in the same manner as in Example 1, but when preparing a grain medium, 6 g of mulberry leaf powder was added and mixed to cultivate P. mushroom mycelium.

<Example 3> The beta-glucan content of the present invention is high cultivation of mushroom mycelia 3

The culture was carried out in the same manner as in Example 1, but when preparing a grain medium, 9 g of mulberry leaf powder was added and mixed to incubate the P. mushroom mycelium.

<Comparative Example 1> Culture of Pseudomonas mushroom mycelium 1

YMA medium (yeast extract 0.3 g, malt extract 0.3 g, glucose 1 g, peptone 0.5 g, Agar 1.8 g, distill water 96.1 mL, pH 5.0) was sterilized at 121°C for 20 minutes, and then a solid medium was prepared.

Fig. 1

Claims (6)

In the cultivation method of the mushroom mycelium,
The first process of manufacturing mulberry leaf powder by pulverizing after drying mulberry leaves,
A second step of preparing a solid medium containing mulberry leaf powder prepared in the first step, inoculating Pseudomonas mushroom mycelium in the medium, and then culturing to prepare a culture,
A third step of preparing a liquid medium containing the mulberry leaf powder prepared in the first step, inoculating the culture of the second step in the medium, and then culturing to prepare a culture solution,
After preparing grains, the fourth process of preparing grain medium by mixing the mulberry leaf powder and grains prepared in the first process at a weight ratio of 1 to 3: 100,
A method for culturing phytophthora mushroom mycelium having a high beta-glucan content, comprising a fifth step of inoculating 3 to 10 ㎖ of the third step of the culture medium per 100 g of the cereal medium to the cereal medium and then culturing the phytophthora.
The method of claim 1,
When preparing the culture of the second step, after preparing a solid medium containing 05 to 3 g of mulberry leaf powder per 100 ml of the solid medium, inoculating Pseudomonas mushroom mycelium, then at 20 to 32 ℃ for 5 to 10 days Characterized in that the culture is produced by culturing, beta-glucan content is high, the cultivation method of mushroom mycelium.
The method of claim 1,
When preparing the culture of the second step, after preparing a solid medium containing 05 to 3 g of mulberry leaf powder per 100 ml of the solid medium, inoculating Pseudomonas mushroom mycelium, then at 20 to 32 ℃ for 5 to 10 days Characterized in that the culture is produced by culturing, beta-glucan content is high, the cultivation method of mushroom mycelium.
The method of claim 1,
When cultivating the mushroom mycelium of the fifth step, it is characterized by incubating for 10 to 25 days at 100 to 1000 Lux, 20 to 32 ℃,
A method for culturing Pseudomonas mushroom mycelium with high beta-glucan content.
The method of claim 1,
In the production of the fourth process of grain medium, it is characterized by using one selected from brown rice, rice, corn, barley, wheat, adlay, sorghum, and soy as grains.
A method for culturing Pseudomonas mushroom mycelium with high beta-glucan content. Pseudomonas mushroom mycelium having a high beta-glucan content, cultured by the method of any one of claims 1 to 5.

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