KR20110130036A - Nanoemulsion composition comprising astaxanthin - Google Patents

Abstract

PURPOSE: A nano-emulsion composition containing astaxanthin is provided to improve the freezing melting stability and the preserving stability, and the water solubility of the composition by mixing the astaxanthin with lysolecithin, sugar ester, auxiliary emulsifier, solubilizing agent, and water. CONSTITUTION: A nano-emulsion composition containing astaxanthin includes 0.05-30 weight% of astaxanthin, 0.05-20 weight% of lysolecithin, 0.1-20 weight% of sugar ester, 1-30 weight% of auxiliary emulsifier, 10-60 weight% of solubilizing agent, and water. A method for manufacturing the nano-emulsion composition containing astaxanthin includes the following: The auxiliary emulsifier and water are mixed. The lysolecithin and the sugar ester are mixed with the mixture and homogenized. The solubilizing agent and the astaxnthin are mixed with the mixture and are homogenized.

Description

Astaxanthin-containing nanoemulsion composition {Nanoemulsion composition comprising astaxanthin}

The present invention relates to an astaxanthin-containing nanoemulsion composition, specifically, astaxanthin 0.05 to 30% by weight, risorecithin 0.05 to 20% by weight, sugar ester 0.1 to 20% by weight, co-emulsifier 1 to 30% by weight, It relates to an astaxanthin-containing nanoemulsion composition consisting of 10 to 60% by weight of a dissolution aid and a balance and a method for preparing the same.

Astaxanthin is a natural carotenoid with a strong antioxidant power, nicknamed supervitamin E. The red flesh of salmon is called salmon pink, and the source of this color is astaxanthin. Astaxanthin is found primarily in the muscles and body surface of seafood. Salmon meat is, of course, rich in eggs. It is also rich in sea bream, sea bream, shrimp and crabs. In vivo, many are present as pigment proteins bound to proteins. When crab is boiled, it becomes red because the pigment breaks down and the color of red astaxanthin appears.

In 1996, according to Shimizu's study, astaxanthin showed 550 times more antioxidant activity than vitamin E. Vitamin E plays an important role in the body's fat-soluble antioxidants. Astaxanthin has helped protect the body from damage caused by lipid peroxidation in mice lacking vitamin E. In addition, astaxanthin showed 100 times greater activity than vitamin E in the inhibition of lipid peroxidation in mitochondria. Antioxidant power also plays an important role in the prevention of atherosclerosis.

Astaxanthin also has an excellent effect on inhibiting anti-inflammatory effects such as anti-stress, lowering diabetes, suppressing complications, preventing cataracts, and inhibiting nephritis. Applying astaxanthin to the back of a person can reduce pigmentation caused by UV and reduce blemishes. This suggests that astaxanthin could be actively used in cosmetics to prevent skin damage caused by UV irradiation. In addition, when LDL cholesterol is oxidized by free radicals, it turns into 'bad cholesterol' and damages the artery wall. Then platelets gather to repair the wound, which attaches to the wall of the artery and becomes thick like a wall, narrowing the artery and creating a blood clot. Especially with the aging of the body, blood vessels and cerebrovascular vessels around the heart called coronary arteries tend to make blood clots. However, astaxanthin blocks the oxidation of LDL cholesterol, which can eliminate bad substances in the blood vessels. On the other hand, a study in which astaxanthin was administered to rats with diabetes has been reported to suppress an increase in blood glucose levels.

In addition, several researchers have recently published papers on astaxanthin intake and eye health. Astaxanthin is reported to be able to prevent and relieve symptoms such as cataracts, reduce eye fatigue, relieve VDT syndrome, improve eye control, and improve vision. In particular, the reason why the VDT syndrome, which is frequently reported to modern people who use the computer, is improved is the main mechanism is that the blood flow to the retina is increased, thereby improving eye control and fatigue.

Astaxanthin has been found to increase its concentration in the blood when consumed by humans, and it is metabolized and used in the body, causing the concentration in the blood to decrease over time. In addition, no side effects and toxicity were reported by the Ames test, single-dose toxicity test, and rat 90-day subchronic (less-than- chronic) toxicity test. It has not been found and is known to be safe for long term intake. In addition, various animals and clinical trials have been conducted, and although the experiment was not intended to evaluate toxicity or safety, no adverse reactions have been reported in various studies, and it is recognized as a safe substance.

Astaxanthin, however, is a high melting point compound (about 160-200 ° C.), insoluble in water, extremely low in solubility in organic solvents, fats and oils, and particularly prone to isomerization by heating, It tends to be easily denatured by heat, oxygen or light. Due to these characteristics, astaxanthin, despite its various usefulness as mentioned above, its utilization is insufficient and its commercialization is limited.

Therefore, there is an urgent need to develop an astaxanthin-containing nanoemulsion composition having high water solubility and excellent stability in various conditions.

Therefore, the present inventors have studied astaxanthin composition having improved storage stability, freeze-melting stability and water solubility, and mixed astaxanthin with risorecithin, sugar ester, co-emulsifier, dissolution aid and water in the form of nanoemulsion. When making, it was found that the water solubility is high, the storage stability, the freeze-melting stability is high, and the heat resistance, acid resistance and flame resistance are also excellent and completed the present invention.

Therefore, an object of the present invention is 0.05 to 30% by weight astaxanthin, 0.05 to 20% by weight risorecithin, 0.1 to 20% by weight sugar ester, 1 to 30% by weight co-emulsifier, 10 to 60% by weight solubilizer and water (balance It is to provide an astaxanthin-containing nanoemulsion composition consisting of).

In addition, another object of the present invention is to (a) mixing the co-emulsifier and water, (b) homogenizing after mixing and adding risorecithin and sugar ester to the mixed product; And (c) to provide a method for producing astaxanthin-containing nanoemulsion composition comprising the step of homogenizing after mixing the dissolution aid and astaxanthin in the mixed product of step (b).

Another object of the present invention is 0.05 to 30% by weight astaxanthin, 0.05 to 20% by weight risorecithin, 0.1 to 20% by weight sugar ester, 1 to 30% by weight co-emulsifier, 10 to 60% by weight solubilizer and water ( It is to provide a cosmetic composition comprising a nano emulsion consisting of a balance) as an active ingredient.

Another object of the present invention is 0.05 to 30% by weight astaxanthin, 0.05 to 20% by weight risorecithin, 0.1 to 20% by weight sugar ester, 1 to 30% by weight co-emulsifier, 10 to 60% by weight solubilizer and water ( It is to provide a food composition comprising a nano emulsion consisting of a balance) as an active ingredient.

In order to achieve the above object, the present invention is 0.05 to 30% by weight astaxanthin, 0.05 to 20% by weight risorecithin, 0.1 to 20% by weight sugar ester, 1 to 30% by weight co-emulsifier, dissolution aid 10 to 60 It provides an astaxanthin-containing nanoemulsion composition consisting of weight percent and balance.

In order to achieve another object of the present invention, the present invention comprises the steps of (a) mixing the co-emulsifier and water, (b) homogenizing after mixing and adding risorecithin and sugar ester to the mixed product; And (c) provides a method for producing astaxanthin-containing nanoemulsion composition comprising the step of homogenizing after mixing the dissolution aid and astaxanthin in the mixed product of step (b).

In order to achieve the other object of the present invention, the present invention is 0.05 to 30% by weight astaxanthin, 0.05 to 20% by weight risorecithin, 0.1 to 20% by weight sugar ester, 1 to 30% by weight co-emulsifier, 10 to 60 solubilizer It provides a cosmetic composition comprising a nanoemulsion consisting of weight% and water (balance) as an active ingredient.

In order to achieve the other object of the present invention, the present invention is 0.05 to 30% by weight astaxanthin, 0.05 to 20% by weight risorecithin, 0.1 to 20% by weight sugar ester, 1 to 30% by weight co-emulsifier, 10 to 60 solubilizer It provides a food composition comprising a nanoemulsion consisting of weight% and water (balance) as an active ingredient.

Hereinafter, the content of the present invention will be described in more detail.

Astaxanthin-containing nanoemulsion composition of the present invention comprises astaxanthin as an active ingredient, characterized in that consisting of risorecithin, sugar esters, co-emulsifiers, dissolution aids and water.

Preferably the astaxanthin-containing nanoemulsion composition of the present invention is 0.05 to 30% by weight astaxanthin, 0.05 to 20% by weight risorecithin, 0.1 to 20% by weight sugar ester, 1 to 30% by weight co-emulsifier, dissolution aid 10 To 60% by weight and water (balance).

When describing the astaxanthin-containing nanoemulsion composition of the present invention as follows.

The composition of the present invention contains astaxanthin as an active ingredient exhibiting various effects. Astaxanthin is a reddish-colored carotenoid that is widely distributed in crustaceans such as shrimp, crab, salmon, and cod, and has a hydroxyl (OH) group and a keto (C = O) group. Have Astaxanthin is expected to have a very strong antioxidant function, and because it reacts well, it is known to exist in the form of protein or fatty acid in nature rather than free form. Astaxanthin of the present invention can be isolated and purified from nature, commercially available and prepared by chemical synthesis methods known in the art. Astaxanthin has a structure similar to carotenoids, but is not converted to vitamin A, and because it is not synthesized in the body of the animal, it is known that the diet should be consumed.

<Formula 1>

Such astaxanthin may be included in an amount of preferably 0.05 to 30% by weight, more preferably 1 to 30% by weight based on the total weight of the composition. If the content of astaxanthin is less than 0.05% by weight, it is difficult to obtain an effect as an active ingredient, and when it contains more than 30% by weight, the emulsion stability is lowered and transparency is impaired.

The composition of the present invention also includes riserecithin as an emulsifier to increase water dispersibility. Such resorcithins impart the preservation stability or cryogenic melting stability of the composition.

Risorecithin of the present invention refers to an enzyme treatment of lecithin using phospholipase, and more preferably, may be increased in purity by solvent extraction after enzyme treatment. Lecithin refers to a mixture of various phospholipids, and the composition of phospholipids may vary depending on their origin. The risorecithin of the present invention may be derived from various origins such as soybean oil, sunflower seed oil and the like, and preferably may be derived from soybean oil. Such risorecithin may be included in an amount of preferably 0.05 to 20% by weight, and more preferably 1 to 15% by weight, based on the total weight of the composition. When the content of risorecithin is included in less than 0.05% by weight, the emulsification effect provided by the lecithin may be weakened and the emulsion stability may be reduced, and when included in excess of 20% by weight, the viscosity of the aqueous composition may be increased. have.

The composition of the present invention also includes sugar esters for the production of stable astaxanthin-containing nanoemulsion compositions. Sugar esters are fatty acid esters of sucrose (sugar), which are excellent in cleaning and emulsifying properties and are used as sugars and fatty acids in the body even when ingested with food. It is nontoxic because it is decomposed and is approved as a food additive. The strong hydrophilicity of sugar residues and the hydrophobicity of the alkali groups of fatty acids maintain a good balance and show excellent emulsifying power. Such sugar esters may be included in an amount of preferably 0.1 to 20% by weight, more preferably 1 to 20% by weight, based on the total weight of the composition. When the sugar ester content is less than 0.1% by weight, the emulsification effect provided by the sugar ester may be weak and the emulsion stability may be lowered. If the sugar ester content is more than 20% by weight, there may be a problem in the physical properties of the aqueous composition.

The composition of the present invention also includes a co-emulsifier to emulsify astaxanthin more stably.

As the co-emulsifier of the present invention, an emulsifier having a HLB (Hydrophilic lipophilic balance) value of 10 or more is preferable, and such an emulsifier is preferably a polyglycerol fatty acid ester. There is no restriction | limiting in particular in the kind of fatty acid, More preferably, hexaglycerol monostearate, hexaglycerin monooleate, hexaglycerol monomyristate (HLB = 13), decaglycerol monostearate, decaglycerol monooleate, decaglycerin Monopalmitate, decaglycerin monomyristate, decaglycerin monolaurate and glycerin esters. In addition, as long as the average HLB is 10 or more, polyglycerol fatty acid ester can use 2 or more types of mixtures. Such co-emulsifier may be included in an amount of preferably 1 to 30% by weight, more preferably 5 to 30% by weight based on the total weight of the composition. When the content of the co-emulsifier is included in less than 1% by weight, a sufficiently transparent emulsion may not be obtained. When the content of the co-emulsifier is included in excess of 30% by weight, the viscosity becomes high and does not become a uniform emulsion, resulting in a decrease in transparency.

The composition of the present invention also includes a dissolution aid to dissolve astaxanthin. The dissolution aid of the present invention refers to a substance that helps dissolution of poorly soluble substances, and preferably, may be propylene glycol, sorbitol, maltitol, sucrose, glucose, fructose, reduced syrup, glycerin, or a mixture thereof. Dissolution aids enable the dissolution of many poorly soluble materials even with relatively small amounts of emulsifiers. Such propylene glycol, sorbitol, maltitol, sucrose, glucose, fructose, reduced syrup, glycerin or a mixture thereof may be included in an amount of preferably 10 to 60% by weight based on the total weight of the composition, more preferably 15 to It may be included in 50% by weight. When the content of the dissolving aid of the present invention is included in less than 10% by weight may reduce the storage stability of the aqueous composition, when contained in more than 60% by weight of the aqueous composition when used in beverages, foods, texture or flavor There may be a problem that is lowered.

The astaxanthin-containing nanoemulsion composition of the present invention has excellent solubility in aqueous solvents including water, and is stable in a wide range of pH, and also stable in heat sterilization and freezing melting, and thus can be used in various formulations for beverages, foods, cosmetics, and functionalities. It can be used for food and medicine.

Therefore, the present invention is 0.05 to 30% by weight astaxanthin, 0.05 to 20% by weight risorecithin, 0.1 to 20% by weight sugar ester, 1 to 30% by weight co-emulsifier, 10 to 60% by weight solubilizer and water (balance) It provides a cosmetic composition comprising the nanoemulsion made as an active ingredient.

In addition, the present invention is 0.05 to 30% by weight astaxanthin, 0.05 to 20% by weight risorecithin, 0.1 to 20% by weight sugar ester, 1 to 30% by weight co-emulsifier, 10 to 60% by weight solubilizer and water (balance) It provides a food composition comprising a nanoemulsion made as an active ingredient.

The cosmetic composition or food composition of the present invention is 0.05 to 30% by weight astaxanthin, 0.05 to 20% by weight risorecithin, 0.1 to 20% by weight sugar ester, 1 to 30% by weight co-emulsifier, 10 to 60% by weight solubilizer and It is characterized in that it comprises a nanoemulsion consisting of water (balance).

The description of the components constituting the nanoemulsion included in the cosmetic composition or the food composition of the present invention is the same as the description of the components of the astaxanthin-containing nanoemulsion composition described above.

The cosmetic composition of the present invention is 0.05 to 30% by weight astaxanthin, 0.05 to 20% by weight risorecithin, 0.1 to 20% by weight sugar ester, 1 to 30% by weight co-emulsifier, 10 to 60% by weight solubilizer and water ( a basic cosmetic composition (cosmetics, creams, essences, cleansing foams and cleansing water) with a nanoemulsion (hereinafter referred to as 'astaxanthin nanoemulsion') as an active ingredient and a dermatologically acceptable excipient. , Pack, body oil), color cosmetic composition (foundation, lipstick, mascara, makeup base), hair product composition (shampoo, rinse, hair conditioner, hair gel) and soap and the like.

The excipients include, but are not limited to, emollients, skin penetration enhancers, colorants, fragrances, emulsifiers, thickeners and solvents. In addition, fragrances, pigments, fungicides, antioxidants, preservatives and moisturizing agents may be further included, and may include thickeners, inorganic salts, synthetic polymer materials and the like for the purpose of improving the properties. For example, when the face wash and the soap are manufactured with the cosmetic composition of the present invention, the astaxanthin nanoemulsion can be easily prepared by adding the face wash and the soap base. When the cream is prepared, it can be prepared by adding astaxanthin nanoemulsion to a cream base of a general oil-in-water type (O / W). To this, synthetic or natural materials, such as proteins, minerals, vitamins, etc., for the purpose of improving physical properties, such as flavors, chelating agents, pigments, antioxidants, and preservatives, may be added.

The content of astaxanthin nanoemulsion contained in the cosmetic composition of the present invention is not limited thereto, but is preferably 0.001 to 10% by weight, more preferably 0.01 to 5% by weight based on the total weight of the composition. If the content is less than 0.001% by weight can not be expected the desired effect, more than 10% by weight may be difficult to manufacture safety or formulation.

Meanwhile, the food composition of the present invention includes all forms such as functional food, nutritional supplement, health food and food additives.

Food compositions of this type can be prepared in various forms according to conventional methods known in the art. For example, the astaxanthin nanoemulsion may be ingested in the form of tea, juice, and drink to be liquefied, granulated, encapsulated, and powdered so as to be drinkable. In addition, functional foods include, but are not limited to, beverages (including alcoholic beverages), fruits and processed foods (e.g. canned fruit, canned food, jams, marmalade, etc.), fish, meat and processed foods (e.g. ham) , Sausage cornbeans, etc., breads and noodles (e.g. udon, soba, ramen, spagate, macaroni, etc.), fruit juices, various drinks, cookies, candy, dairy products (e.g. butter, cheese), edible vegetable oils It can be prepared by adding astaxanthin nanoemulsion to margarine, vegetable protein, retort food, frozen food, various seasonings (eg, miso, soy sauce, sauce, etc.). In addition, in order to use the astaxanthin nanoemulsion in the form of a food additive, it may be prepared and used in the form of a powder or a concentrate.

The preferred content of the astaxanthin nanoemulsion in the food composition of the present invention is not limited thereto, but is preferably 0.01 to 50% by weight in the finally prepared food.

On the other hand the present invention comprises the steps of (a) mixing the co-emulsifier and water; (b) homogenizing after mixing and adding risorecithin and sugar ester to the resultant mixture; And (c) provides a method for producing astaxanthin-containing nanoemulsion composition comprising the step of homogenizing after mixing the dissolution aid and astaxanthin in the mixed product of step (b).

Hereinafter, the method for preparing the composition of the present invention will be described in detail step by step.

(a) mixing the co-emulsifier and water

In the step (a), an emulsifier having a HLB (Hydrophilic lipophilic balance) value of 10 or more is put into water and melted and mixed. The mixing may be by a conventional mixing method known in the art, for example, may be stirring using a stirrer or mixing using a homomixer.

The temperature at the time of melting and mixing is not limited thereto, but may be preferably 40 ° C. to 80 ° C.

(b) adding homogenized risorecithin and sugar ester to the mixed product of step (a) and homogenizing

In the step (b), risorecithin and sugar ester are added to the resultant mixture obtained in the step (a), mixed and homogenized. The mixing may be by a conventional mixing method known in the art, for example, may be stirring using a stirrer or mixing using a homomixer.

The temperature at the time of melting and mixing is not limited thereto, but may be preferably 40 ° C. to 80 ° C.

(c) adding homogeneous dissolution aid and astaxanthin to the mixed product of step (b) and homogenizing

In the step (c), the dissolution aid and the astaxanthin are added to the resultant mixture produced in the step (b) and mixed and homogenized.

The mixing may be by a conventional emulsion mixing method, for example, may be mixing using a homomixer. The homogenization may be preferably performed using an ultrahigh pressure homogenizer, and the homogenization pressure may vary depending on the component content of the composition of the present invention, preferably 500 to 3000 bar. The homogenization process can be completed once and can be repeated several times as necessary.

In one embodiment of the present invention, astaxanthin-containing nanoemulsion compositions were prepared in various compositions.

In one experimental example of the present invention, the properties of the astaxanthin-containing nanoemulsion composition of the present invention were observed. As a result, it was confirmed that the astaxanthin-containing nanoemulsion composition of the present invention did not exhibit delamination or precipitation phenomenon (see Experimental Example 1).

In another experimental example of the present invention, the water solubility of the astaxanthin-containing nanoemulsion composition of the present invention was measured. The composition of the present invention was mixed in distilled water, and then its transparency was measured. As a result, it was confirmed that the astaxanthin-containing nanoemulsion composition of the present invention was well dissolved in distilled water, and the solution had high transparency. As a result, the composition of the present invention was confirmed to be well dissolved in an aqueous solvent (see Experimental Example 2).

In another experimental example of the present invention, the heat resistance, acid resistance and flame resistance of the astaxanthin-containing nanoemulsion composition of the present invention were tested. The composition of the present invention was added to distilled water adjusted to pH 3 to 11 and heated at 95 ° C. for 5 hours, or heated at 121 ° C. for 30 minutes, and then the particle size change of the astaxanthin-containing nanoemulsion composition in the composition was measured. As a result, the astaxanthin-containing nanoemulsion composition of the present invention was stable even in acidic and basic conditions, and showed no significant change in particle size even when heated to 95 ° C. for 5 hours or at 2 bar pressure for 30 minutes at 121 ° C. It confirmed (see Experimental Example 3).

In another experimental example of the present invention, the freeze melting stability of the astaxanthin-containing nanoemulsion composition of the present invention was tested. The astaxanthin-containing nanoemulsion composition of the present invention was freeze-stored at -20 ° C and then taken out at regular intervals to measure the particle size. As a result, it was confirmed that the astaxanthin-containing nanoemulsion composition of the present invention did not show a significant change in particle size until 30 days and was stably preserved and maintained (see Experimental Example 4).

In another experimental example of the present invention, the long-term stability of the astaxanthin-containing nanoemulsion composition of the present invention was tested. Astaxanthin content of the astaxanthin-containing nanoemulsion composition of the present invention was measured, and then stored in an incubator at 4 ° C, 25 ° C and 40 ° C for 1 year, and then the astaxanthin content was measured again. As a result, it was confirmed that the astaxanthin-containing nanoemulsion composition of the present invention stably preserves the content of astaxanthin at all temperatures regardless of the storage period (see Example 5).

As described above, the present invention provides an astaxanthin-containing nanoemulsion composition comprising astaxanthin as an active ingredient and consisting of risorecithin, sugar esters, co-emulsifiers, dissolution aids, and water. The composition of the present invention improves the water solubility of astaxanthin, which is excellent in storage stability, freeze-melting stability, and excellent in heat resistance, acid resistance, and flame resistance, and is effective in preparing functional foods, pharmaceuticals, and cosmetics.

1 is a graph of the heat resistance, acid resistance and flame resistance measurement results of the astaxanthin-containing nanoemulsion composition of the present invention (control: a composition diluted in distilled water, heat treatment: a composition diluted in distilled water and heated at 95 ° C. for 5 hours, pH3 to pH11: composition diluted with distilled water adjusted to each pH and then heated to 95 ° C for 5 hours.
Figure 2 is a graph of the measurement results of heat resistance, acid resistance and flame resistance of the astaxanthin-containing nanoemulsion composition of the present invention (control: a composition diluted in distilled water, heat treatment: diluted in distilled water and heated to 121 ℃ for 30 minutes under 2 bar pressure) One composition, pH 3 to pH 11: composition diluted in distilled water adjusted to each pH and then heated to 121 ° C. for 30 minutes under 2 bar pressure).
Figure 3 is a graph of the results of the freezing melting stability test of the astaxanthin-containing nanoemulsion composition of the present invention.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

<Examples>

Astaxanthin-containing nanoemulsion composition

Astaxanthin-containing nanoemulsion composition was prepared by the following method with the composition according to [Table 1].

Add co-emulsifier and water, mix and add risorecithin and sugar ester to the resultant mixture, homogenize with homomixer (PRIMIX Corporation, TK Robomics, Japan), and then add dissolution aid and astaxanthin according to [Table 1]. After sufficiently stirring with a homomixer, the mixture was passed three times in succession at 500 to 2,000 bar in an ultrahigh pressure homogenizer (Microfluidics, M-110, USA), and then, aseptically filtered and subdivided.

Components of Astaxanthin-Containing Nanoemulsion Compositions (weight%) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Astaxanthin 0.05-20% by weight 0.05-20% by weight 0.05-20% by weight 0.05-20% by weight 0.05-20% by weight 0.05-20% by weight Risorecithin 1-20% by weight 1-20% by weight 1-20% by weight 1-20% by weight 1-20% by weight 1-20% by weight Sugar ester 0.1-20 wt% 0.1-20 wt% 0.1-20 wt% 0.1-20 wt% 0.1-20 wt% 0.1-20 wt% Co-emulsifier (polyglycerin fatty acid ester) 20-30% by weight 20-30% by weight 20-30% by weight 20-30% by weight 20-30% by weight 20-30% by weight glycerin 10-60 wt% 5-30% by weight 5-30% by weight 5-30% by weight 5-30% by weight 5-30% by weight Propylene glycol 5-30% by weight Sorbitol 5-30% by weight Maltitol 5-30% by weight Glocoose 5-30% by weight Fructose 5-30% by weight Water (to make 100% by weight in total) balance balance balance balance balance balance

< Experimental Example  1>

Astaxanthin  contain Of nanoemulsion  Constellation observation

100 ml of the astaxanthin-containing nanoemulsion composition prepared in Examples 1 to 6 was placed in a brown sample bottle and placed in a refrigerated (4 ° C.) room temperature (25 ° C.) for 1 year without external impact or irritation, followed by layer separation. Or it was observed whether precipitation occurred.

As a result, as shown in [Table 2], it was confirmed that the astaxanthin-containing nanoemulsion composition of the present invention did not exhibit delamination or precipitation.

Astaxanthin-containing nanoemulsion composition appearance results sample Refrigerated (4 ℃) Room temperature (25 ℃) Separation Precipitation Separation Precipitation Example 1 none none none none Example 2 none none none none Example 3 none none none none Example 4 none none none none Example 5 none none none none Example 6 none none none none

< Experimental Example  2>

Water solubility test ( After melting  transparency( transparency ) Measure)

The astaxanthin-containing nanoemulsion composition of the present invention prepared in Examples 1 to 6 was added to 50 mL of distilled water so as to add 10 mg of astaxanthin, the mixture was stirred gently, and then the transparency thereof was measured. As a comparative example, 10 mg of astaxanthin was added to 50 ml of distilled water and mixed in the same manner, and then the transparency was measured.

Transparency was visually observed and evaluated in three stages as follows.

***: Melting well, solution is transparent

**: Melted well but the solution is cloudy

*: Precipitates or suspended solids are present

As a result, it was confirmed that the astaxanthin-containing nanoemulsion composition of the present invention was well dissolved in an aqueous solvent as shown in [Table 3].

Transparency Evaluation Results of Astaxanthin-Containing Nanoemulsion Compositions sample Evaluation results Example 1 *** Example 2 *** Example 3 *** Example 4 *** Example 5 *** Example 6 *** Comparative Example 1 *

< Experimental Example  3>

Astaxanthin  contain Nano Emulsion  Heat resistance of the composition, Acid resistance  And flame resistance test

After adding the astaxanthin-containing nanoemulsion composition of the present invention prepared in <Example 1> so that 10 mg of astaxanthin was added to 50 mL of distilled water adjusted to pH 3, 5, 7, 9 and 11, the mixture was gently stirred and mixed. Heat treatment was performed at 95 ° C. for 5 hours, or at 121 ° C. for 30 minutes under 2 bar pressure. After heat treatment, the particle size of the astaxanthin nanoemulsion in the composition was measured.

Astaxanthin-containing nanoemulsion composition particle size measurement was measured using a particle size analyzer (Nanotrac, NPA-210, Montgomeryville, PA 18936, USA) and measured as a total of three times the average value.

The particle size of the astaxanthin nanoemulsion of the heated composition without the pH control as a control and the composition not treated after the preparation was measured in the same manner.

As a result, as shown in FIG. 1 or 2, the astaxanthin-containing nanoemulsion composition of the present invention was stable even in acidic and basic conditions, and heated to 95 ° C. for 5 hours or 30 ° at 121 ° C. under 2 bar pressure. It was confirmed that no significant change in particle size was observed even after heating for a minute.

< Experimental Example  4>

Astaxanthin  contain Nano Emulsion  Freeze-melting stability test of the composition

The astaxanthin-containing nanoemulsion composition prepared in Example 1 was mixed well and then subdivided into seven tubes and stored at -20 ° C to freeze. 0, 1, 3, 5, 10, 15, 30 days from the start of the experiment was taken out one by one to measure the particle size of the emulsion. The same test was carried out three times and the average value was taken.

As a result, astaxanthin-containing nanoemulsion composition of the present invention as shown in FIG. 3 did not show a significant change in the astaxanthin nanoemulsion particle size in the composition until 30 days, it was confirmed that it is stably preserved and maintained.

< Experimental Example  5>

Astaxanthin  contain Nano Emulsion  Long-term stability test of the composition

Long-term stability test was conducted to confirm the stability of the astaxanthin-containing nanoemulsion composition of the present invention.

After measuring the astaxanthin content of the astaxanthin-containing nanoemulsion composition prepared in Examples 1 to 6, divided into 50 mL tubes and stored in 4 ℃, 25 ℃ and 40 ℃ constant temperature, respectively, the same after 1 year Astaxanthin content was measured by the method.

As a result, as shown in [Table 4], it was confirmed that the astaxanthin-containing nanoemulsion composition of the present invention was stably preserved astaxanthin content at all temperatures regardless of storage period.

The stability of astaxanthin was calculated by the following formula.

Stability = (astaxanthin concentration in emulsion measured at 1 year) / (astaxanthin concentration in emulsion measured before experiment) * 100

Long-term stability evaluation results of astaxanthin-containing nanoemulsion composition stability 4 ℃ 25 ℃ 40 ℃ Early 1 year Early 1 year Early 1 year Example 1 100 100 100 100 100 100 Example 2 100 100 100 100 100 100 Example 3 100 100 100 100 100 100 Example 4 100 100 100 100 100 100 Example 5 100 100 100 100 100 100 Example 6 100 100 100 100 100 100

Accordingly, the present invention provides an astaxanthin-containing nanoemulsion composition comprising astaxanthin as an active ingredient and consisting of risorecithin, sugar esters, co-emulsifiers, dissolution aids, and water. The composition of the present invention increases the water solubility of astaxanthin, which is water insoluble, has excellent storage stability, freeze-melting stability, and excellent acid resistance, flame resistance, and heat resistance, which is effective in manufacturing functional foods, pharmaceuticals, and cosmetics, and thus has high industrial applicability. .

Claims (8)

Astaxanthin consisting of 0.05 to 30% by weight of astaxanthin, 0.05 to 20% by weight of resorcithin, 0.1 to 20% by weight of sugar ester, 1 to 30% by weight of co-emulsifier, 10 to 60% by weight of dissolution aid and balance Containing nanoemulsion compositions.
The astaxanthin-containing nanoparticles of claim 1, wherein the dissolution aid is any one selected from the group consisting of propylene glycol, sorbitol, maltitol, sucrose, glucose, fructose, reduced syrup, glycerin, and mixtures thereof. Emulsion composition.
According to claim 1, wherein the co-emulsifier has a HLB of 10 or more hexaglycerin monostearate, hexaglycerin monooleate, hexaglycerin monomyristate (HLB = 13), decaglycerin monostearate, decaglycerol monooleate, An astaxanthin-containing nanoemulsion composition, characterized in that it is at least one selected from the group consisting of decaglycerin monopalmitate, decaglycerin monomyristate, decaglycerin monolaurate and glycerin esters.

(a) mixing the co-emulsifier and water;
(b) homogenizing after mixing and adding risorecithin and sugar ester to the resultant mixture; And
(c) adding homogeneous dissolution aid and astaxanthin to the resultant mixture of step (b) and homogenizing
Astaxanthin-containing nanoemulsion composition manufacturing method comprising a.
A nanoemulsion comprising 0.05 to 30% by weight of astaxanthin, 0.05 to 20% by weight of resorcithin, 0.1 to 20% by weight of sugar ester, 1 to 30% by weight of co-emulsifier, 10 to 60% by weight of dissolution aid and balance Cosmetic composition containing as an active ingredient.
A nanoemulsion comprising 0.05 to 30% by weight of astaxanthin, 0.05 to 20% by weight of resorcithin, 0.1 to 20% by weight of sugar ester, 1 to 30% by weight of co-emulsifier, 10 to 60% by weight of dissolution aid and balance Food composition comprising as an active ingredient.
The composition of claim 5 or 6, wherein the dissolution aid is any one selected from the group consisting of propylene glycol, sorbitol, maltitol, sucrose, glucose, fructose, reduced syrup, glycerin, and mixtures thereof. .
7. The co-emulsifying agent according to claim 5 or 6, wherein the co-emulsifier has a HLB of 10 or more, hexaglycerin monostearate, hexaglycerin monooleate, hexaglycerin monomyristate (HLB = 13), decaglycerin monostearate, decaglycerin A composition comprising at least one selected from the group consisting of monooleate, decaglycerin monopalmitate, decaglycerin monomyristate, decaglycerin monolaurate and glycerin esters.

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