KR20110126888A - A natural composition for mosquito repelleat having excellence durability - Google Patents

Abstract

PURPOSE: A mosquito repellent composition is provided to keep the repelling effect for seven or more hours without skin irritation. CONSTITUTION: A natural mosquito repellent composition contains Eucalyptus extract oil and Portulaca oleracea etract as a main ingredient. The composition also contains aloe vera extract, citronella oil, cinnamon oil, lavender oil, or rosemary oil and hydrotalcite. The particle size of the hydrotalcite is 3um or less.

Description

A natural composition for mosquito repelleat having excellence durability}

The present invention relates to a natural mosquito repellent having excellent mosquito repellent activity and a sustaining effect, and more particularly, to an extract oil prepared from a natural plant or a compound separated from the extract oil, in particular a double layered compound. By prescribing modified hydrotalcite, the duration and effect of mosquito repellent activity is very long and it relates to an excellent natural mosquito repellent.

Mosquitoes, one of the main pests that cause direct and indirect damage, are known to have about 50 types of mosquitoes in Korea alone. The main reason is the state. Among these mosquitoes, Aedes togoi mediates filamentous fever in the southern islands, Culex triaeniorhynchus spreads Japanese encephalitis, and the Chinese spotted mosquito (Anopheles sinensis) is responsible for malaria and inland vulgaris. Inland Brugian Filariasis, which is a family of Culx pipiens pallens and Culx pipiens molestus, has recently risen in apartment complexes, and has recently emerged as a mediator and blood-sucking problem of many infectious diseases. There is a situation.

The demand for mosquito repellents that can effectively prevent insect-borne diseases and have a low environmental impact has increased. Conventionally known repellents include citronella oil, dimethylphthalate (DMP), etc., but lack repellent activity. The mosquito-repelling plants reported so far include plants of the genus neem oil, Lantana camara, Artemisia vulgaris and Eucalyptus.

In particular, insect repellents (PMDs) derived from lemon eucalyptus are manufactured using oil residues extracted from lemon eucalyptus plants in China. Although PMD is not as effective as DEET (N, N-diethyl-m-toluamide), it has been shown to be effective against anopheles and also effective against ticks, flies and mosquitoes. In addition, PMD is not reactive with plastics and synthetic fibers, so it can be applied to various products and has very low toxicity. (Oral test in rats LD 50 2,408 mg / kg, transdermal LD 50> 2,000 mg / kg) DEET is a synthetic repellent that has excellent repelling effect and is used throughout pests, but it has an unpleasant irritant odor and plastic It can't be used to react to synthetic rubber, and especially, it has a side effect that can cause brain disease, seizures, cardiovascular toxicity and hypersensitivity due to its large skin penetration ability. It has a hard disadvantage. (Hongchun et al., A review. J. Am. Mosq. Control Assoc. 14 (1): 12-27, 1998)

Therefore, in recent years, research on natural extracts or compounds derived from natural products has been actively conducted. The repellent, developed as a new concept using natural extracts, utilizes natural substances that inhibit the behavior of living organisms as a pest control agent, which can prevent insect-borne diseases on humans and have no side effects such as environmental pollution. However, repellents are demanding because they are intended for human use and must include the function of controlling them to stimulate the chemosensory of insects.

Necessary requirements for repellent should be harmless to human body, long lasting effect, no irritation to skin, no unpleasant odor, chemically stable, effective even if not treated all over skin Etc.

In particular, the mosquito repellents prepared on the basis of the mosquito repellents and natural materials used in combination with DEET shows the mosquito repellent effect, but there is a problem that the duration of the effect is about 1-2 hours short. New methods of protection or alternative medicines are being developed New humans will be faced with difficult situations if they cannot be effectively used because of the very short time they can be free from mosquitoes. Due to the problem that the duration of the effect is very short, in particular, the addition of fatty acids such as stearic compounds and stearic acid to have a coating effect on the main material can be recognized as a solution in terms of the duration. However, it may lead to the improvement of the dispersing effect by fatty acids, but the reduction of the repelling effect and the non-ideality of the shape, there is a problem that it is difficult to manufacture in aerosol shape when applied to the spray. In addition, natural mosquito repellents that show excellent repellent activity while improving sustainability have not been developed.

It is an object of the present invention to provide a natural mosquito repellent which is excellent in mosquito repellent activity, has no toxicity to humans or animals, has a simple treatment method, has no irritating odor, and has a very long lasting repellent effect.

The inventors of the present invention have a short duration of 1 to 2 hours of mosquito repellents that are conventionally used, and when applied to the skin, it causes skin irritation and mosquito repellents manufactured on the basis of natural substances. It was found that the effect was much lower than the repellent effect such as DEET. The inventors of the present invention have a layered support carrier which can play a role of slowly releasing the main component of the mosquito repellent in the composition by releasing the main component of the mosquito repellent in the layer, thereby forming a mosquito repellent. The present invention was completed by confirming that the repellency rate of 100% duration can be extended by 7 hours without inhibiting the mosquito repelling effect.

Subject to surround the metal cation is an octahedral structure with the basic unit, ^- double the hydrotalcite type of layered compound is a kind of a metal hydroxide, a divalent or trivalent metal cation is located in the center of the center 6 of hydroxyl ion (OH) Repeated octahedral units have a double layer structure that forms two layers. Anion and water molecules are positioned between the double layers to maintain the equilibrium of charge. [M ^II _{1- x} M ^III _x (OH) ₂ (A ^n- ) _{2 /} nmH ₂ O], where M ^II is a divalent metal, M ^III is a trivalent metal, and A ^{n -} is n It is an anion having a valence. The ion of hydrotalcite intermediate layer has been used as the ion trapping ability of the ion trapping ability having excellent heat resistance and weather resistance when processing the resin by the ability to exchange anions under various conditions. The present invention can use all of the various depositable methods, and can achieve the above object in the most effective and efficient manner depending on the conditions. The size of the secondary particles of the synthesized hydrotalcite can be used all hydrotalcite synthesized in various sizes up to 0.1㎛-10㎛.

Such hydrotalcite of the present invention has a small and uniform size, and when processed to be applied to gel and spray forms of mosquito repellents, smooth processability, skin irritation and repellency persistence are usually 90% of secondary aggregated particle size. In the case of the powder (d (0.9)), the powder is preferably about 10 μm or less, preferably about 5 μm or less, and the size of the secondary aggregated particles is about 3 μm or less on average. However, it is only possible to predict that the effect will be halved when using particles having a size smaller than 50 nm as a support.

According to the present invention, hydrotalcite containing modified eucalyptus oil acts as a repellent lasting agent as a carrier while maintaining mosquito repellent activity as a mosquito repellent, thereby maintaining the duration of drug efficacy at least 100% of the repellency rate for at least 7 hours. To a mosquito repellent composition.

Eucalyptus oil extracted from lemon eucalyptus oil is represented by the following chemical formula (1). In addition, the chemical formula of hydrotalcite having a layered structure serving as a carrier is represented by the formula (2) and the chemical structure of PMD (p-menthane-3,8-diol) which can be derived from eucalyptus oil.

[Mg_{1 - x}Al_x(OH)₂(CO₃ ^2-)·mH₂O]

[Mg _{1 - x} Al _x (OH) ₂ (CO ₃ ^2- ) mH ₂ O]

Expression (1) Expression (2)

           Expression (3) Expression (4) Expression (5) Expression (6)

PMD undergoes hydroxylation from eucalyptus oil to form 3-hydroxy cineole (Eq. (6)), which produces PMDs by eliminating ethoxy groups. P-menthane-1,8-diol, etc. (Eq. (5)) made through oxidation of eucalyptus oil (Eucalyptol) as well as PMD made in this way are all ethoxy in a hydrotalcite mixture solution that can form a basic compound with water. It is thought that it can be formed in an atmosphere that can lead to physical reaction with the destruction of the group. The hydroxyl group on the surface of the hydrotalcite is predicted to be trapped by the modification of the eucalyptus oil and the surface physics reaction, and the reaction that invades into the layer of the layered compound by the anion exchange reaction occurs by the physical bonding. It is believed that a rather unstable compound was formed.

As such, hydrotalcite is a mosquito repellent excipient that acts as a carrier by physical bonding with eucalyptus oil in the molecule and externally, and when the mosquito repellent is applied to the skin or sprayed, it is gradually released by body temperature and ambient temperature. Inference can be inferred, and the results are in good agreement.

The mosquito repellent composition relates to a mosquito repellent composition mainly composed of eucalyptus oil prepared by mixing hydrotalcite as 0.1 to 5.0 wt% as a repellent sustaining agent carrier. In the present invention, wt% is based on the total amount. This sustained effect can be explained by the ethoxy reaction with the following three hydrotalcites. It is anticipated that three possible materials can be achieved by the above mechanism. In addition, the three materials are expected to coexist at the same time. There may be a case where the hydroxy group on the outer surface of the hydrotalcite particle is attached by physical reaction and the anion resulting from the cracking of the alkoxy group is intercalated in the middle of the hydrotalcite layer. There may be cases where there is a simultaneous interlayer. Eucalyptus oils present in the bonds and layers are thought to flow out first from the surface and continue out from the inside.

The amount of hydrotalcite may be used in an amount of 0.1wt% to 5wt%, but preferably 0.5 to 3.0wt%, and when it is 5wt% or more, it is possible to change the viscosity of the entire repellent solution, which is preferable because the entanglement of the carrier appears. I can't. In addition, it was determined that slowing the dissolution rate would have a great effect on the repelling effect, but the results of the study did not affect the repelling effect at all, and only by slowing the dissolution rate, it has a role of maintaining the repelling effect for a long time.

[Table 1] and [Table 2] showed the repelling effect and the repelling time in different compositions.

The well-known mosquito repellent derived from the well-known natural material has a good repellency rate, but has a short time to avoid the evacuation time of about 1 or 2 hours, and has the disadvantage of spraying the repellent every hour. The present invention provides a mosquito repellent composition mainly composed of eucalyptus oil and machi oil to maintain a repellent time of about 7 hours or more by changing the components of natural mosquito repellents and adding excipients such as hydrotalcite in order to compensate for this drawback. There is.

Larvae breeding methods, adult fables and spawning

Aedes albopictus collected from Gaduk-myeon, Cheongwon-gun, Chungbuk, Korea, was used as a silkworm breeder in a breeding room under a temperature of 25 ± 2 ° C, a humidity of 70 ± 5%, and a 16L: 8D condition in Gwangju. The adults were bred with sugar water (5%) in a 40 × 27 × 30cm breeding net, and a rat was used as a vampire animal. The spawned egg masses were collected and transferred to a plastic breeding container (23 × 30 × 7cm) filled with groundwater. After hatching, the larvae were fed with a bake of prey, and the pupa was delivered every day to adult breeding nets. Allegorized. Hieroglyphs for evaluating evasion force were used to make a hole with a toshi on the wall to insert two hands in a box of cuboid 60 × 30 × 36 cm in height. It was. Test specimens were maintained at a constant temperature and humidity before and during the experiment.

<Evaluation of Evasion Effect by Human Hand>

It was performed by three experimenters (26-30 years old, male), and the left hand of each experimenter was treated as a treatment and the right hand was untreated. Before the drug treatment, the experimenter's hands were sterilized with dilute ethanol, washed with running water and dried. When the repellent was treated, about 0.5 g of the test drug was applied to the left hand of each experimenter and spread evenly on the back of the hand and palm. 50 adult white mosquito mosquitoes were spun on the prepared mosquito gland epithelial device, and both hands were exposed for 5 minutes up to 8 hours at 1 hour intervals immediately after the repellent treatment. Evacuation rate was determined by counting the number of vampires of each hand during the exposed time. The mosquitoes used in the experiment were collected within 5 generations, and female adults of 4-6 days of hatching were used.

<Data Analysis>

The evacuation rate was determined by the blood absorption of treated and untreated groups.

The present invention will be described in detail with reference to the following Examples.

 <Production of Mosquito Repellent Composition>

Example  One

A: By mixing in 5.0 g glycerin, 5.0 g mineral oil, 4.0 g stearin compound, 30.0 g purified water, 3.0 g of propylene glycol, 0.2 g methyl paraben, and 1.0 g aloe vera leaf extract are added to the excipient mixture. Got it.

B: 10.0 g of lemon eucalyptus oil and 2.0 g of portuguese extract were added to 30.0 g of purified water, and the mixture was sufficiently mixed with the excipient mixture A to complete a mosquito repellent. The mosquito repellent effect test was performed using the mosquito repellent composition obtained here, and the result is shown in following [Table 1].

Example  2

Excipients were prepared as A of Example 1 and B of Example 1 was mixed with hydrotalcite 1.0 wt% based on the total mosquito repellent composition at room temperature for about 30 minutes to obtain a white mosquito repellent. The mosquito repellent effect test was performed using the mosquito repellent composition obtained here, and the result is shown in following [Table 1].

Example  3

Excipients were prepared as A of Example 1 and B of Example 1 was mixed with hydrotalcite 3.0 wt% based on the total mosquito repellent composition for about 30 minutes at room temperature to obtain a white mosquito repellent. The mosquito repellent effect test was performed using the mosquito repellent composition obtained here, and the result is shown in following [Table 1].

Example  4

Excipients were prepared as A of Example 1 and B of Example 1 was mixed with hydrotalcite 5.0 wt% based on the total mosquito repellent composition at room temperature for about 30 minutes to obtain a white mosquito repellent. The mosquito repellent effect test was carried out using the mosquito repellent composition obtained here, and the result is shown in following [Table 1].

TABLE 1

  TABLE 2

The mosquito repellent composition of the present invention is prepared by adding hydrotalcite and vegetable extract ingredient citronella, eucarbide oil, and extract of machi, and mixing steric compound, stearic acid, propylene glycol, ethyl alcohol, methyl paraben, etc. as excipients thereto. As a result, the repellency rate of mosquito repellents is very high and it is effective to maintain the duration for at least 7 hours for a long time.

Although the present invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical spirit of the present invention, and such modifications and modifications belong to the appended claims.

Claims (4)

In the composition, a lemon eucalyptus leaf (Eucalyptus) extract oil, a mosquito repellent composition mainly composed of Portulaca oleracea extract, aloe vera leaf extract, citronella oil, cinnamon oil, cinnamon oil, lavender oil, and rose mario oil, which are vegetable extracts, And a natural mosquito repellent composition, which is then formulated by mixing hydrotalcite with 0.01 to 5.0% by weight based on the total composition. The mosquito repellent of Claim 1 which contains 0.5 to 3.0 weight% of hydrotalcite with respect to the whole composition in the mosquito repellent composition of Claim 1. The method of claim 2, wherein the was has an average secondary particle diameter of less than 3㎛ of the hydrotalcite particles, hydrotalcite, characterized in that 90% has a particle size distribution curve within a 10 μm (D _{0 .9)} . The hydrotalcite according to claim 1, wherein the heat treatment is performed at 80 to 250 ° C. for 1.5 to 2.0 hours.