KR20110041668A - Single nucleotide polymorphism markers in swine and method for determination of domestic pork origin by using the same - Google Patents

Abstract

PURPOSE: A SNP marker which is used for determining the place of origin of domestic pork is provided to trace pork history. CONSTITUTION: A polynucleotide for determining the place of origin of domestic pork or a complementary polynucleotide thereof is novel 51 SNP markers selected from a pig group. The polynucleotide is obtained from muscle, skin, blood, bone, or other organs. A method for determining the origin of domestic pig comprises: a step of isolating nucleic acid from pork; a step of confirming base type of a marker of SNP at a nucleotide of sequence numbers 1-51 and storing the database; and a step of analyzing the place of origin of the pork using a parental marker which is already stored.

Description

Single Nucleotide Polymorphism Markers in Swine and Method for Determination of Domestic Pork Origin by Using the Same}

The present invention relates to a single nucleotide polymorphism marker of pigs and a method of determining the origin of domestic pork using the same.

Domestic and international studies using DNA markers to provide accurate information on individual identification and lineage in livestock are being done. In the past, paternity discrimination and individual tracking were performed using microsatellite markers, but high analytical prices and high error rates in the reading process have been highlighted as problems.

However, recent research has shown that genotypes can be analyzed at low cost using single nucleotide polymorphism (SNP) markers and applied to livestock traceability or origin identification (Anderson and Garza, 2006; Rohrer et al. , 2007; Baruch and Weller, 2008). In 2009, Takeshi Honda conducted a simulation in cattle, selecting 50 useful single-base polymorphic markers (MAF: q, 0.25≤q≤0.35) to estimate 98-99% paternity estimators in a small cohort. Presented.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

The present inventors found 51 new mononucleotide polymorphism markers in swine population and performed efficiency verification for individual identification using them, and it is possible to accurately predict the lineage relationship as a result of applying the 51 markers to identify the origin of pork. The present invention was completed by experimentally confirming.

Accordingly, it is an object of the present invention to provide a mononucleotide polymorphism marker of swine.

Another object of the present invention is to provide a method for determining the origin of domestic pork using the marker.

The objects and advantages of the invention will become apparent from the following detailed description, claims and drawings.

According to an aspect of the present invention, the present invention provides a 264th nucleotide of SEQ ID NO: 1, 287 nucleotides of SEQ ID NO: 2, 496 nucleotides of SEQ ID NO: 3, 219 nucleotides of SEQ ID NO: 4 , 256th nucleotide of SEQ ID NO: 5 sequence, 290th nucleotide of SEQ ID NO: 6 sequence, 267th nucleotide of SEQ ID NO: 7 sequence, 256 nucleotides of SEQ ID NO: 8 sequence, 370th nucleotide of SEQ ID NO: 9 sequence 117th nucleotide of SEQ ID NO: 10, 171 nucleotides of SEQ ID NO: 11, 143 nucleotides of SEQ ID NO: 12, 196 nucleotides of SEQ ID NO: 13, 295 nucleotides of SEQ ID NO: 14 , 228th nucleotide of SEQ ID NO: 15 Sequence, SEQ ID NO: 143 nucleotides of SEQ ID NO: 16, 131 nucleotides of SEQ ID NO: 17, SEQ ID NO: 423 nucleotides of SEQ ID NO: 18, 256 nucleotides of SEQ ID NO: 19, 230 nucleotides of SEQ ID NO: 20, SEQ ID NO: 247th nucleotide of the 21st sequence, 225th nucleotide of the 22nd sequence, 384th nucleotide of the 23rd sequence, 431th nucleotide of the 24th sequence, 272th nucleotide of the 25th sequence, 306th nucleotide of SEQ ID NO: 26, 327 nucleotide of SEQ ID NO: 27, 344 nucleotide of SEQ ID NO: 28, 109 nucleotide of SEQ ID NO: 29, 349 nucleotide of SEQ ID NO: 30, SEQ ID NO: 115th nucleotide of the 31st sequence, 256 nucleotides of SEQ ID NO: 32, 411 nucleotides of SEQ ID NO: 33, 198 nucleotides of SEQ ID NO: 34, 171 nucleotides of SEQ ID NO: 35, 378 nucleotides of SEQ ID NO: 36, 237th nucleotide of SEQ ID NO: 37, 239 nucleotide of SEQ ID NO: 38, 61 nucleotide of SEQ ID NO: 39, 251 nucleotide of SEQ ID NO: 40, 256 nucleotide of SEQ ID NO: 41, 215 nucleotides of SEQ ID NO: 42, 269 nucleotides of SEQ ID NO: 43, 290 nucleotides of SEQ ID NO: 44, 256 nucleotides of SEQ ID NO: 45, 58 nucleotides of SEQ ID NO: 46, 263 th nucleotide of the 47 th sequence , 8-100 consecutive nucleotides comprising the 184th nucleotide of SEQ ID NO: 48 sequence, the 171th nucleotide of SEQ ID NO: 49 sequence, the 287th nucleotide of SEQ ID NO: 50 sequence, or the 175th nucleotide of SEQ ID NO: 51 sequence And polynucleotides or complementary polynucleotides thereof useful for determining the origin of pork.

The present inventors discovered 51 novel mononucleotide polymorphism markers in swine population and performed efficiency verification for individual identification using them, and it is possible to accurately predict the lineage relationship as a result of applying the 51 markers for identifying the origin of pork. The present invention was completed by experimentally confirming.

The SNP marker of the present invention is applied to swine, most preferably to domestic pigs.

As used herein, the term “nucleotide” is a deoxyribonucleotide or ribonucleotide that exists in single- or double-stranded form and includes analogs of natural nucleotides unless otherwise specified (Scheit, Nucleotide Analogs, John Wiley, New York (1980); Uhlman and Peyman, Chemical Reviews, 90: 543-584 (1990).

As used herein, the term "nucleic acid molecule" has the meaning of comprehensively including DNA (gDNA and cDNA) and RNA molecules, and the nucleotides, which are the basic structural units in nucleic acid molecules, are naturally modified nucleotides, as well as modified sugar or base sites. Analogs (Scheit, Nucleotide Analogs, John Wiley, New York (1980); Uhlman and Peyman, Chemical Reviews, 90: 543-584 (1990))

According to another aspect of the present invention, the present invention provides a method for determining the origin of pork, comprising the steps of: (a) separating nucleic acid molecules from pork to be traced to origin; (b) identifying the base type of the marker of Single Nucleotide Polymorphism (SNP) corresponding to the nucleotide of the designated position of SEQ ID NO: 1 to 51 of the sequence described in claim 1 and storing it in the database step; And (c) analyzing the origin of the pork using the markers of the parents already stored.

In the method of the present invention, the nucleic acid molecule can be obtained from various sources of pork, for example from muscle, epidermis, blood, bone, organ, and most preferably from muscle or blood.

If the starting material in the process of the invention is gDNA, isolation of gDNA can be carried out according to conventional methods known in the art (Rogers & Bendich (1994)). If the starting material is mRNA, total RNA is isolated and carried out by conventional methods known in the art. See Sambrook, J. et al., Molecular Cloning. A Laboratory Manual, 3rd ed. Cold Spring Harbor Press ( Ausubel, FM et al., Current Protocols in Molecular Biology, John Willey & Sons (1987); and Chomczynski, P. et al., Anal. Biochem. 162: 156 (1987)). The isolated total RNA is synthesized into cDNA using reverse transcriptase. Since the total RNA is isolated from animal cells, it has a poly-A tail at the end of the mRNA, and cDNA can be easily synthesized using oligo dT primer and reverse transcriptase using this sequence characteristic (see PNAS). USA, 85: 8998 (1988); Libert F, et al., Science, 244: 569 (1989); and Sambrook, J. et al., Molecular Cloning.A Laboratory Manual, 3rd ed.Cold Spring Harbor Press (2001) )).

In the method of the present invention, step (b) may be carried out by applying various methods known in the art used to identify specific sequences. For example, techniques applicable to the present invention include fluorescence phosphorylation hybridization (FISH), direct DNA sequencing, PFGE analysis, Southern blot analysis, single-strand conformation analysis (SSCA, Orita et al., PNAS, USA 86: 2776 (1989)), RNase protection assay (Finkelstein et al., Genomics, 7: 167 (1990)), dot blot analysis, denaturation gradient gel electrophoresis (DGGE, Wartell et al., Nucl. Acids Res. 18: 2699 (1990)), methods using proteins that recognize nucleotide mismatches (e.g., mutS protein of E. coli) (Modrich, Ann. Rev. Genet., 25: 229-253 (1991)), and Allele-specific PCR, including but not limited to.

Sequence changes result in differences in base bonds within single-stranded molecules, resulting in different bands of mobility, which SSCA detects. DGGE analysis uses denaturation gradient gels to detect sequences that exhibit mobility different from wild-type sequences. Other techniques generally employ probes or primers that are complementary to sequences comprising the SNPs of the invention. For example, in RNase protection assays riboprobes complementary to the sequences comprising the SNPs of the invention are used. DNA or mRNA isolated from the riboprobe and the plant are hybridized and then cleaved with an RNase A enzyme capable of detecting mismatches. If there is a mismatch and RNase A recognizes, a smaller band is observed.

In assays using hybridization signals, probes complementary to the sequences comprising the SNPs of the invention are used. In this technique, hybridization signals of probes and target sequences are detected to directly determine whether SNP variants are present.

As used herein, the term “probe” refers to a linear oligomer having naturally occurring or modified monomers or bonds, including deoxyribonucleotides and ribonucleotides that can hybridize to a particular nucleotide sequence. Preferably, the probe is single stranded for maximum efficiency in hybridization. The probe is preferably deoxyribonucleotide.

As the probe used in the present invention, a sequence perfectly complementary to the sequence including the SNP may be used, but a sequence complementarily complementary to a range that does not prevent specific hybridization may be used. It may be.

Preferably, the probe used in the present invention is a sequence capable of hybridizing to a sequence comprising 8-100 consecutive nucleotides including SNP nucleotides of any one of SEQ ID NOs: 1 to 51 It includes. More preferably, the 3'-end or 5'-end of the probe has a base complementary to the SNP base. In general, since the stability of duplexes formed by hybridization tends to be determined by the consensus of the sequences of the ends, the ends in probes having bases complementary to the SNP base at the 3'- or 5'-ends If the parts are not hybridized, these duplexes can be dismantled under stringent conditions. Conditions suitable for hybridization include Joseph Sambrook, et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (2001) and Haymes, BD, et al., Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, DC Decisions may be made with reference to those disclosed in (1985). Stringent conditions used for hybridization can be determined by adjusting the temperature, ionic strength (buffer concentration), the presence of compounds such as organic solvents, and the like. Such stringent conditions can be determined differently depending on the sequence being hybridized.

According to a preferred embodiment of the present invention, such gene amplification basically uses a primer pair designed to amplify polynucleotides including the SNP nucleotides of the present invention.

A "primer" in the context of the present invention is a single strand of oligonucleotide, suitable conditions (the presence of four different nucleoside triphosphates and polymerases such as DNA or RNA polymerase), suitable temperatures and suitable It is meant to act as an initiation point that can initiate template-directed DNA synthesis under a buffer.

The suitable length of the primer is determined by the nature of the primer to be used, but is usually used as a length of 15 to 30 bp. The primers need not be exactly complementary to the sequence of the template but must be complementary enough to form a hybrid-complex with the template.

Amplification techniques that can be used in the methods of the invention include PCR amplification (Miller, HI (WO 89/06700) and Davey, C. et al. (EP 329,822)), ligase chain reactions (LCR, Wu, DY) et al., Genomics 4: 560 (1989)), polymerase ligase chain reaction (Barany, PCR Methods and Applic., 1: 5-16 (1991)), Gap-LCR (WO 90/01069), repair chain Reactions (EP 439,182), 3SR (Kwoh et al., PNAS, USA, 86: 1173 (1989)) and NASBA (US Pat. No. 5,130,238). Most preferably according to the PCR amplification step. Where amplification is applied, it is important to design suitable primers to identify the SNP bases of the present invention. The conditions of the amplification reaction by PCR and the reagents and enzymes used may be those commonly known in the art.

As described in detail above, the present invention provides a single nucleotide polymorphism (SNP) marker that can be usefully used for determining the origin of domestic pork, a method for determining the origin of domestic pork using the same, and a kit used in the method. It is about. Using the SNP marker by the single nucleotide variation provided in the present invention, not only can generate unique DNA fingerprint information of individual pigs to track the origin of the pork product, but also identify the sows and sows through the farm origin. It is possible to confirm and trace the history from pig production to consumption of pork products.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example

Example  1.Identification and pedigree estimation of pigs DNA Marker contents Efficiency analysis

Selection of efficient DNA markers using livestock identification and lineage estimation is based on the independence between the markers and the Hardy-Weinberg equilibrium. Only when these two conditions are satisfied, the maximum likelihood value or LOD value, which represents the result of paternity discrimination and paternity analysis of real data, is meaningful. However, when a large number of markers are to be performed, some markers are not independent because they are located close to the same chromosome, and the number of data is small or the Hardy-Weinberg may not be satisfied depending on the characteristics of the markers. In this case, the DNA marker contents that can be used for the paternity of pigs can be constructed only if the markers satisfying the Hardy-Weinberg equilibrium conditions or independent markers are selected before performing paternity analysis.

Example 2 Efficacy of DNA Marker Selection for Parental Estimation of Livestock is Likelihood Method for Analyzing Polymorphism Information between Markers (Barrett JC et al., 2005).

2-1. Hardy-Wineburg Equilibrium ( HWE ) Verification

The purpose of the Hardy-Wineberg equilibrium test is to check whether the sample used in the experiment is well screened or whether the genotype or genotype frequency of the population remains constant. In addition, genotype frequency can be explained using genotype frequency by the law that genotype frequency and genotype frequency remain unchanged even after generation changes, unless there is an element that changes genotype frequency in a large group that crosses randomly. .

HardyWineberg Equilibrium Expectations

MM = p ² x N; MN = ² pq x N; NN = q ² x N

MM, MN, NN: genotypes corresponding to each

In practice, the difference between the observed value and the expected value at Hardywinberg equilibrium is evaluated using the χ ² test method.

χ ² = ∑ ((observed-expected) ² / expected

This value is not significant when the F-value corresponding to the significance level of 1% is less than 3.84 (P-value <0.01) when the degree of freedom is 1 in the χ ² distribution table, that is, between the observed value and the expected value. There is no significant difference, so we can assess the Hardywinberg equilibrium of the population.

2-2. Heterozygosity Verification

The simplest way to determine the degree of genetic variation at a particular locus is to measure the degree of heterozygosity. Hetrozygosity is a heterozygosity given by the probability that the genotype of a randomly selected person is heterozygous for a particular locus.

H = 1-p ² -q ² = 2p (1-p)

In the above expression, in the case of bi-allele markers such as SNP, the genotype frequency of the SNP marker is called p and q (q = 1-q), respectively.

In general, the higher the heterozygosity marker, the more informative marker. As shown in Table 1, the SNP marker developed in the present patent was able to observe a high polymorphic information index in the genotype population.

Table 1 Markers Used for Individual Identification and Percentage Testing in Pigs

Example 3 Efficacy of DNA Marker Selection Using the Parental Estimation of Livestock The Stephens Method for Independence Analysis between Markers (Stephens er al., 2001).

The two most commonly used methods for measuring independence between markers are D 'and r ² Is to use a value. Both measurements have values between 0 (no linkage) and 1 (complete linkage).

1) [D ']-D prime

A ₁ B ₁ = p ₁ xq ₁

A ₁ B ₂ = p ₁ xq ₂

A ₂ B ₁ = p ₂ xq ₁

A ₂ B ₂ = p ₂ xq ₂

A, B: each gene

p _1, q _1, p _2, q ₂ ; Each allele

The formula for calculating the D value is as follows.

D = P ₁₁ P ₂₂ -P ₁₂ P ₂₁

If the two markers are perfectly related, the value of D takes 0. However, the degree of association takes other values, and D 'is a measure that is generally used more than D.

D '= D / D _max ,

D _max = min (p ¹ q ² , p ² q ¹ ), if D> 0

max (-p ¹ , q ¹ , -p ² q ² ), if D <0

D _max ; The maximum value of D is shown.

2) r ² -r-square

Another method that can be measured by verifying association and independence between markers is r ^2. There is a method using (correlation), which is a measure of how statistical correlation is between two markers.

r ² =

p ¹ q ² , p ² q ¹ ; Each allele.

As shown in Table 2, none of the markers associated with each marker could be found (r2> 0.3).

TABLE 2 Correlation between 51 SNP Markers in Swine

Example 4. Estimation of paternity in pig populations using 51 SNP markers (1st and 2nd generation).

4-1. Paternity presumption genotyping

Genotypes were analyzed to estimate first-generation progeny and second-generation paternity, and the CERVUS 3.0 program, currently widely used, was analyzed using ().

4-2. Biological Statistical Analysis

The PIC value of each marker was calculated using the Botstein method as follows.

PIC = 1-∑ni = 1pi2-2∑n-1i = ∑nj = I + 1pi + 2pj2

pi and pj are the probability that alleles of "1" and "2" appear, and n is the number of alleles.

The paternity index (PI) and probability of paternity (PP) were used for the probabilistic expression of paternity using the Aickin method.

1) Statistical frequency (SF): ∂

2) Paternity Index (PI) or Likelyhood Ratio (LR): β / ∂

3) Probability of Paternity (PP): [β / (β + ∂)] x 100 (%)

In this case, SF is the probability (∂) of being a paternity when randomly selecting individuals from the entire population. PI or LR is the ratio (β / ∂) of the probability that the randomly selected individual is paternal (β) to the probability that the individual is paternal (β). PP is a percentage of PI.

Statistical Summary

Confidence intervals of 95% to 100% were shown as significant intervals, and the genotypes of 62 genotypes of the first generation showed 100% of genotype agreements between father and mother information. By comparing the presumed lineage relationship between the first generation and the second generation, which were previously identified by the original tag, through the statistical analysis of 51 single-base polymorphisms, it was observed that all of them matched.

Example  5. 51 SNP Markers  Estimation of paternity in pig populations (2nd and 3rd generation).

5-1. Genotyping for Estimating 2nd Generation Progeny and 3rd Generation Parents

Genotypes were analyzed to estimate the second generation progeny and the third generation of paternity. As a paternity discrimination program using DNA markers, CERVUS 3.0, which is widely used, was analyzed using ().

<Intact pedigree output file>

Offspring ID; Entity ID of the descendant

Typed loci: The number of markers in which genotype values were recorded in the target object.

5-2. Biological Statistical Analysis

The PIC value of each marker was calculated using the Botstein method as follows.

PIC = 1-∑ni = 1pi2-2∑n-1i = ∑nj = I + 1pi + 2pj2

pi and pj are the probability that alleles of "1" and "2" appear, and n is the number of alleles.

The paternity index (PI) and the probability of paternity (PP) were used for the probabilistic expression of paternity by using the Aickin method.

(1) Statistical frequency (SF): ∂

(2) Paternity Index (PI) or Likelyhood Ratio (LR): β / ∂

(3) Probability of Paternity (PP): [β / (β + ∂)] x 100 (%)

In this case, SF is the probability (∂) that can be a bias when randomly selecting individuals from the entire population. PI or LR is the ratio (β / ∂) of the probability that the randomly selected subject is the father (β) to the probability of the father being the father (β). PP is a percentage of PI.

<Statistic summary>

Confidence intervals of 95%-100% resulted from the statistical results above, and 346 genotypes of 2nd generation progeny showed 314 heads of 100% genotypes of father and mother information. When the percentage matched more than%, both father and mother information estimated 346 parents. From the statistical analysis, 51 single base polymorphic markers were used for estimating kinship relations, and the identification rate was 98.463% to 98.463%.

<Statistic summary>

As a result of the above statistical analysis, 51 markers were used to estimate the parent of fathers and mothers who did not know the mother information, which was almost 100%. The result showed higher estimation power than previously reported results.

Example 6 PCR Primers

Information on the nucleotide sequence of the PCR primer used in the SNP analysis of the present invention is as follows.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

<110> Chungbuk National University Industry Academic Cooperation Foundation <120> Single Nucleotide Polymorphism Markers in Swine and Method for          Determination of Demestic Pork Origin by Using the Same <160> 51 <170> KopatentIn 1.71 <210> 1 <211> 358 <212> DNA <213> Pig <400> 1 ttcacaggcg atgcctatgt catcctgaag acggtgcagc tgaggaacgg gaacctgcag 60 tatgacctcc actactggct gggtgaggct ggcccccacc ccrgtccctc cacctccacc 120 ccagcccctc tgctgcagag cacagacctt tgagtcatgg tctccaggaa gcgcccatga 180 gaattacggg actgttgagc atcaagtgtg tgccaggccc ttggttatgt gttcccaaga 240 ctctagagcc acctgcccaa gttyagactt cagctctgcc ctttggcaag atatccagtg 300 tctctgtgaa gtggacatga tgatcgcacc agaccacctc ataggactgt tgtgaggg 358 <210> 2 <211> 470 <212> DNA <213> Pig <400> 2 gagaaagtcc agactcggct ggaggagacc caggcgctgc tgcggaagaa ggagaagtca 60 ggcaccttcc cgggcccctg ctycctgcac ccccctcatr ccagcagctc cctgcctcct 120 agcccaggcc cttctgtagt aagactctcc ccagggggac agacttcctt ccccattgat 180 ggccccctgg tctcattgca rtyggagcct cttctgcccc tggctcctca ccagcagggc 240 tactccaagg acctgtcagg actgagaatt aggggcagag ccacggkgaa gggacactgc 300 ccaagcccgg gttctcttcc gcagagagtt tgaggagacg atggatgcac ttcaggctga 360 cattgaccag ctggaggcag agaaggcaga gttaaagcaa cggctgaaca gccagtccaa 420 gcgcacaatc gaggggatcc ggggaccccc tccctcgggt attgctaccc 470 <210> 3 <211> 551 <212> DNA <213> Pig <400> 3 gtgaggtaat cctttatttt cttacattca cttttgaagt attgacttcc tttttttagt 60 ctkttgtgat atatgcagat atcacccaaa gtaaaatgtr ttgagaaaat rgcagagctt 120 taacatctgc tgaacttctg aaattattaa ttttaatttt agattgtact gtgctctttt 180 gtttccacta ctgttgagca aasgaaaaca ccgatgtaga aaaaaagtag ctttgctcta 240 ttgtgcacta cagaatagta gagacacatt tttattagca gctgtggaaa aaaaatgttt 300 ctttttgtag gaggggcatt acagcagctt ttgtgccatc cctaatccat tttaaaattt 360 atttctaggg aaacagtgct cttaaaacat tggaaatgaa tccttgtacc ccaaataatg 420 ttgaggttct ggaaactcga tcagcagttc ctaaaataga agatacagaa ataatctctc 480 cagtagctga gcgtcyggaa gagagctctg atgcagaacc cgaaaaccat gtggttgtat 540 cctattgtcc a 551 <210> 4 <211> 474 <212> DNA <213> Pig <400> 4 gggggccggt gctctgacaa ccccgaggga gggtacacct gccgctgccc tgggggcttc 60 tctggcttta actgtgagaa gaagatggat tcctgcactt cctcaccctg ttccaatggt 120 aagggggcca cctgacccac ttgagacttg gtcagctggt cgacactgac gaggacgtgg 180 caggagcaca ggaccttagt tttatgctga gctcatcayt ttgtgagcta ccttgcattt 240 caggagctct tttgtctgag cagtgagaaa cttcctagca agtctttcct ggagtctggg 300 aacacccagg gagtgagaaa ggcaggcagg tgtgttcctt ctctgtgagg atgcctcagg 360 cagagagatg cagtgatttc tcctggtgcc tcaggtgcgg gagaccccag ggatccgcga 420 gtcagcgcca gtgccagccc agcctctctg ctttaggagg gaagcgcagc tgta 474 <210> 5 <211> 511 <212> DNA <213> Pig <400> 5 tgccatgaat ggtgatgaag gttgcagatg tggctcatat cccacattam wgtggctgtg 60 actgtggccg gcagctgcag ctcatatttg acccctagcc tgggaatctc catatgctgc 120 aggtgcaacc ctaaaaagca aaaaacaaaa aacaaaaaac aaaaccccaa aaaacaagat 180 caacttagac tttcattcta taaggacaga gagactacag tgaaaggaca gtaagcacat 240 gtatccagtg cttatratgt gctaggttct ctttttaagt gatgtactaa ctcatttaat 300 ccctaagaca atcctgtgag gttagggaac ttgcccatag tcagaacatt tttctggggg 360 cagttatccc ttgtcaaggt tcctgcaggg gaggatccct ggccgtggta gcagctgctg 420 cttttctaat gtggggactt ggtatcctgc ctagctaagc ctgagggtga aggctttgaa 480 gcggcaggtg gatgaagcag aagaggaaat t 511 <210> 6 <211> 358 <212> DNA <213> pig <400> 6 ttcacaggcg atgcctatgt catcctgaag acggtgcagc tgaggaacgg gaacctgcag 60 tatgacctcc actactggct gggtgaggct ggcccccacc ccrgtccctc cacctccacc 120 ccagcccctc tgctgcagag cacagacctt tgagtcatgg tctccaggaa gcgcccatga 180 gaattacggg actgttgagc atcaagtgtg tgccaggccc ttggttatgt gttcccaaga 240 ctctagagcc acctgcccaa gttcagactt cagctctgcc ctttggcaar atatccagtg 300 tctctgtgaa gtggacatga tgatcgcacc agaccacctc ataggactgt tgtgaggg 358 <210> 7 <211> 469 <212> DNA <213> pig <400> 7 aggyactact gctactcccg ctattgagag gaggaaactg aggctcaggc taagagaact 60 ggccaaggtc cagggctgac aaaaggcaga ggtggggtcc aagccaggct ttttccaacc 120 gcccacctgg cccttcttgc ttcctctgtg tgttgtccty atttatagga caggttgggg 180 cagagccgga gagaaaccca gacacctggt gctagctagt gggaaggcag gcccaagccc 240 ccagcacctc ccagcygctg cggaagsggt tttctggatg accgatgcca cagaatctgg 300 tttgggaatc ccatyrgcac ccccyycatc tccaaactgc cctgagtcta ggcacccaca 360 gggctctggg gtaygtaggg ggaccatacc tggggtgggg tcacataatc agacacgtcc 420 atgactcggt tggcatagcg tccaaagccc ttcaccttgg tcaccaccg 469 <210> 8 <211> 563 <212> DNA <213> pig <400> 8 atcttctctc ggctggcatt gagccacagc atgtaggcag acatgggcct cttgggggca 60 ttgggatctt tgcccttctt cacctgcaga ggaacccaaa tggcttcagc catctatttc 120 ccacaaaccc ttgcattctc ccaaataaat tatttggatc gcctgcttaa ggggataaaa 180 tggaaaaaag cctaaacagc accaagccag actgagagct tcccaagggc aaagaccatg 240 cccttctccc ctctayaccc cactgtcatg ctccttaggg gcccctttcc ttgggctccc 300 aggacagagg aagcctgctc ctacttccga tctcccagaa gctaattatc ycaaggactt 360 tcaaagatgc tcctcaaggc catcatcact ggctacaaaa acccacctag aggaccrrtc 420 agcaggccct atggtggggg acagaagaag gtggcttccc ttccccaamg gccgcccccc 480 gcccccttag aggaggccgc acctctgtgg gcttcttgcg gctcttgcga tccttggcca 540 tcttggcctt tttaagctgc ttc 563 <210> 9 <211> 550 <212> DNA <213> pig <400> 9 gtggcagaac ctggggggca gtgcggagca cagggagtca gagcccaggc actctgccct 60 cttccagtac ctgctgtggg cctactgtgt gacaggtact aggaaaagag cagaaagctg 120 gacagaccca ctgcccagcc tcctgcagcc ctggagtttg gggagggcac ccacccgcct 180 gccctagcag gttatccaay aacagctcag cctcccacca tggaggaayg gctttcccat 240 ccatggaggg atggctgagg ctgcctggtc cacagcccga ggccgggtga ggacaggttc 300 cttggcgttc ccctgrtttc cctctccaga ggtcctgaga tgctgtggtc caactggaca 360 gagaggggty ggtgggactc acggttgccg tagcccacga gcagcacagc atggtcgatg 420 agccaagtgg tcgatgagct caggcggcct ngggctgtgg accaggcagc ctcagccatc 480 cctccatgga tgggaaagcc attcctccat ggtgggaggc tgagctgttg ttggataacc 540 tgctagggca 550 <210> 10 <211> 372 <212> DNA <213> pig <400> 10 aacgaggagt gtccccggct cagtggggca ggggcctgaa ccgaagccct ggtggcaagg 60 gaagcctgtc cagcactcag aggggggcca yttatactga tctcagccag ggacacrctc 120 tcctccttcc aggatcttct cccatccgaa cgtgctcccg gtgctgggtg cctgtcagtc 180 gccacctgct cctcacccga ccctcatcac acactggatg ccatacggat ccctgtacaa 240 cgtgctacac gagggcacca gtgagtcggg gaggccagcc ctcgccgagg gagcgggagg 300 ggaggcgtgt gcgcctcgcc agacggtctg actcttggct cccctctcag attttgtcgt 360 ggaccagagc ca 372 <210> 11 <211> 521 <212> DNA <213> pig <400> 11 gtaaggagac gctggacacc atgctggatg ggctgggccg catccgagac cagctttctg 60 ccgtggccaa caagtgagcc actgcccggt tgctcctggg cacccacctc ccagcacrtg 120 gtagccaccg ccacaggcac cagtggggac aaaggaggct gccacccaga mggcacagtc 180 ggaccagagg cagccaggcc cctacatgga tgttctcrct ttttccccct cttcccgaag 240 ttggtgagca taagcygctc tcctcmcagg gccctgtgtg gtacctgact cccagcatgg 300 cggggagarg gaccttgctt ggtcagctgc ctctgsctgg ctgattggaa aattcaggcc 360 caaattgtgg agctcgtttc cagctctgaa gacctctggg gagtcaggtg gctgatgcca 420 gcctgcttct ctatcagcga agccaggcag atcccgcagg gcagggccac tcaaacttac 480 ctgctgcagc gtgccygtcc caccctggtc cccagccttc r 521 <210> 12 <211> 489 <212> DNA <213> pig <400> 12 ctacgttccc aacatgctcg ggatcatccc ctatgctggg atagacctcg ccgtctacga 60 ggtgagccct ggaggactcc agccccttga gcagcacaag agactcagag cccccctccc 120 agccgtgggt cagacacccc gcygggtaga ccccctgggg accagcraga tcagaggctg 180 gtctcctcgc cggctctyct gtctgttgca agggtcyccc gtcacacygc ctgrcccccg 240 cacccccagg ttctgtaccc ccgagggctg ggcgggcaga ctccggagca caggagtcgg 300 gctccctggg gagggaaagg ggggtgctgt ctctgccccc tccccgctcc ccaccttatg 360 caggaatccc tagtgacagc aaaagcaaag ggcaggcggg ccaggagctc agcaagctcc 420 aggccaaccc aggccaagcg aattgggtta cagtggcctc ccgcagatyc catgtgatgg 480 ggaaacgcc 489 <210> 13 <211> 451 <212> DNA <213> pig <400> 13 agacccaagg cggggaatgg aactgttgac atttccggct tacccgatgt gagatactct 60 gcactgctcc aggaagactt tgcctattca gggtttgagt gttgggtaga aaatgaggat 120 cagattcagg agctacatag ctgtcatgct tcggaargcc ctggtaaccg acccaggaga 180 tgccgtggct gtgcarcttt gcgggttgca gattctgact atgaagctat ttgtaaggtg 240 cctcgaaagg tggccaaaag tatctcatgt ggcccttcta ccaggtggtc caccagcatt 300 tgcactgaag aaccagcttt gtctgaagtt gggccaccgg atttatcaat ttctaaggtt 360 cccccagatg gagaaagcat ggaggaagag acaccgggat cctctgtgga gtctttggat 420 gcaagtgtcc acgctagtcc tccacaacaa a 451 <210> 14 <211> 454 <212> DNA <213> Pig <400> 14 taccgcgtgc agggccgggc ggtgctgccc atccggtgga tggcctggga gtgtatcctc 60 atggtgagag gccctgccac aggcaggcag ggaggggagg ttaggggaac actkgctgcc 120 actcaaagct ctgggatctc attcagtcac ttgccttccc tggactgcat ttcctcatct 180 ctaaagtgaa ggtttggayc aatgctctat acgggagctc aagggactgg agaaagcagg 240 gaggtatggt gtgatgggaa agggtcttac aagagcgagt gtgagagatg gagtsagggc 300 agcagagagc aagagagata aggaggctag aaacagaagg caatgagttg ggagtcagga 360 ccagaaaatg ggggaaggat gggtagaaac gggaagcggt gaggactaga gccagagaag 420 agcagcaggc ttagtgggag agggccagct aagg 454 <210> 15 <211> 608 <212> DNA <213> pig <400> 15 ctgattccct ctctttctcc tataaactac tccgacctga aagttttcaa gagatgggac 60 atgggacatt gctctgctct cattaaggtg agacatatgg cagtctttct ctctgtcctt 120 ctttctgttc cacccagact tttttgatat gtcactcacg tatagcaaca tctcatcatt 180 cccagctata gctacatgtc agctcatggg ttcctctatc acaaaacyta agatctggaa 240 ggaactgatg gaaggaattg aaggtacatg atgacctctc tggttcttcc atcactttct 300 gctatgctgt atgtattttc cactcctatg ttccagaatc ttccattttg gaagctagac 360 agagtctttc tgttatgtgc atattaaagg ttaattttct gataataaat atagcaatag 420 ccaccttaat tgacacaatc cctgtacayg ttctayattt gtagccactt atctgtatac 480 ccctgtgcca ttccctgtga ttctgcctat aggttcttcc tggatttgag aacatctttt 540 ttgctcactc aagctggtac acatatgcag ccatgctcag gatatataaa cactgggact 600 tcaacatc 608 <210> 16 <211> 468 <212> DNA <213> pig <400> 16 ccacacaact acctgagctg aatggctgcc ccaaacaggt cttggtgagt tgtccttgtt 60 ttcttggagt ggaggcygcg gggggtgggg cagacccctt gggagtcagg gagacccagc 120 cttaaygagc ctgtcataaa gcktcctggg tgaagcactg ccttcagctc tyggcccacr 180 gaacatgctg ctgacggctg aatgcatgtg ggncagtgaa acagcaaaca ygccacttcc 240 taagagtcct ctcctttgga gagaagcatt ctagcctkta aggcaactcc tctgcttcct 300 atttgctctt ctaccatcaa atagccttct gcctctgttt tcctaggatt catgggggag 360 tcagcagaaa agtyccgagc atgacagaca acargatgcc cgcttcactc ccccattttc 420 tctaactgtt gtttttcttt ctcatctctc tcccctgaac aaagaaat 468 <210> 17 <211> 393 <212> DNA <213> pig <400> 17 atgtagtcgg gggtgtagcc cattccaaag agggagctgg tgggatgcag gtggcarggc 60 atacctgtcc ggatattcac atactcccca attccctagg gaagagaccc agcaggtgac 120 cgcatcagca maacaccsca cacctggcca gctaccccat cccttcatac tgttttgaca 180 ccggcttttg ggggagcccc agggttgcag gctgtacaat gagaaaggaa gaactctgcc 240 ctggtacaaa gcatcaggcc aggaaggggc tcaccttgag cttggctgcc tggtgaaaat 300 argcagcaca gatacacttc ctgacaatgt cccagtcggt gccacacgag gccaggctca 360 tccgctgctg caccatgatg tccttgagct gaa 393 <210> 18 <211> 508 <212> DNA <213> pig <400> 18 gctggcggct gtgtggcatc gtgagctggg gcaccggctg tgccctggcc caaaagccag 60 gcgtctacac caaagtcagt gacttccggg agtggatctt ccaggccata aaggtgaaay 120 ttgagtccag atgggagcca gagggcagga gggtttggga caccaarcaa gaaagggggg 180 agttcccact gtggctcagt gggttaagaa cccaactaat agccatgaag atggatayta 240 gtttgatccc yggtctcact cagtgggtta aggatccaaa gttgcttcca cctgtggtgt 300 aggcyggcag ytgcagctcc aattcaaccc ctagcctggg agcttccata tgccacragt 360 gtagccctaa aataaatcaa taagggagaa agggaggcag ggttttctgg aaacctatgc 420 tcyggcttga aagacaggtg catttcagag ggctcctggg aaggggaggt cagccatggg 480 cctttagggg ggaagcctgt cagaccca 508 <210> 19 <211> 402 <212> DNA <213> pig <400> 19 gtttcgccca gaacattcca ggcctggatc ttgctggccc tgagatagaa gtctaggggt 60 gagcggccag ctgccccatc gatggcagga cctctaagga gggagaggag aggagacagg 120 gcgaggggag gggagcccac cttcacagat gtcccaggca caccaggggt gttctgcaga 180 ggggtcctca gcctctgggt ggcgcaggtg gagcagggcc tgcacaggga ttcgggaggc 240 caggtagccc acggcrgtgt agggctcctg gatttgggca atggggtaga tccccgccag 300 gacctggggg aaacaaggac actcagcacc ttcctccatc tcctgcctcc tcccgcccgg 360 ccccctccrc cctcatacct gcagctgcct gggcagaagc ga 402 <210> 20 <211> 440 <212> DNA <213> pig <400> 20 ttgtccctgg cttgtagaat accaccgcca ggatgtcact agcaccccct gctggattga 60 gatccatctg cacggccccc tccagtggct ggataaagtt cttactcaaa tgggttcacc 120 tcacaatcct atttcgtctg tgtcttaagc ggcctgggct tctgcctctt gaaaactatt 180 gagccttgca tgtacttgaa ggatggatga gtcagacacc atctgaacts acaaaggagc 240 cttgataata cttgacctct gtgaccaact gatggattca gaaatttgaa aaccttggta 300 acatactgtt gatattgaga acctgtttag tttacatggt aacattctat ggtaaaatca 360 actaaaatgc atacttttag caggacttcg tgtatagtta aagaagagat ggccaagcca 420 gggacaaaat atctacatcc 440 <210> 21 <211> 431 <212> DNA <213> pig <400> 21 agagctgctg ggaggccctg gtcagccccc tggtgcagaa cgtcacctcc cccggtaact 60 gccccgcccc gcactagcct ctcgcgtggc cctgctcacc ggccccttct tgctctactc 120 tagagccacc tgggaaatgc tcttggcctt tttggtaccc acgttcgttt ggtatccatg 180 tgttcccctt ctcacagccc aggggcygcc tcctttcccc tgtcttccac gtgccagctg 240 ccaccayaag gtcaargggg tccttgcaac cttccttacc gtatggttct gtcccgttgc 300 cctttctctg cactcctgtc cagcccagct tccctgctgg cctgcagacg cgctctgcct 360 tggcttgttt tcccccrccc ccagatgagg atggcatcag ccccatgggc tggctgctgg 420 accagtacct g 431 <210> 22 <211> 413 <212> DNA <213> pig <400> 22 caagtgttag ttttaacaat gaggtttggg atcccgggca gctgagctct gaccgcagat 60 ccagtgaggg ccctgcttct gggcccctgg acccagggga gccccgcags ctccaggtga 120 caggagcgaa gggggtggcc ttcagccrtg aggatgagcg ggaacctctg ccrgcaccgg 180 aggasggagk tgggaragca ggcgtggacg gggacgctcc caccyrcccg aggccgcctt 240 gtgcccgagg ttagctgaac acggtgttgt gctgctgggt cacccggatg aaggtcgccc 300 tccggctgag ctctttgagy ttggcggccc ctacgtaggt gcaggtagac ctcagtcccc 360 cgagaatatc cagaatcgtg ttctccacgt cccctttgta aggtacttcc acg 413 <210> 23 <211> 568 <212> DNA <213> pig <400> 23 catacaagac cattacacgc tgctggaacg tcagatcatc atcgaggtta gactcctccg 60 tagtctctct gctttctgct tcagtgccag tgttcygtgc cattcagcat ctaggaggct 120 tctgcccaca ttttgcacac tttcatttcc taagagcctt tcattttctc tctctctgcr 180 tggyagtctt ccctccattg tcctcacgca tccgtttcct ctcacttgct gttgacctgg 240 aagtatyccc tcgtcatcct cgaactgcgc ttcagcgctg ctgctccggt cctcctccat 300 ccaactggac ctgtcatcct ggtgtgggct ttaagctccc ctgggcaacc ctaggcactg 360 ccttcttctc ttcctcccct cctkcctagc gtaggaaccc agtgcggtag tattaaagac 420 ttccctcckg cctctcctcc atgagcattg ggcttcctcc accacaggca aatgatcgtc 480 atctggagtc agcaggrcag acggagatct tccggaagca cccccgcaaa gcgtccatcc 540 tcaacatgcc gttagtgacg acgctctt 568 <210> 24 <211> 611 <212> DNA <213> pig <400> 24 cattgcaggc atcgccctgg tgcagcayca gcagggcctc gccaacccag gtgctgcccc 60 aggacccttc actctcactt cagaagtggg ttattcccag cgctgcccca gacctcttcc 120 tacactggcc cccagcgttt tcacctcctg cccagggttc tctgtgaaga tgtattrtaa 180 ttcccatcac ccccatttta gagtcgagga aactggrtct ctcacccagg agtccctycc 240 cagatggtgg tggacctggg actagcacca gtctgatctt tgccctttcc tgctcacccc 300 cagccccagc acttagcacc ctgaccccac ccacctgcct tctacttgtc atcccttgca 360 gcctctccag gcccaccacg cayccctcag catccagctt cctgtgggcc tggtctgtgt 420 tctgcatctt ycctgcttca gggctggctt cctttaaatg cccacggtgc acagctccgg 480 attcttctgt tctgcccact cctccccaca cgcctcccag catccacgcc tcccctagcg 540 ctggtgttcc cctcgcccag gtctggtggg cctgtcgcta tcctacgccc tgtccctgac 600 gggcctgtct c 611 <210> 25 <211> 353 <212> DNA <213> pig <400> 25 ttcggaaaaa tactgctatt tgcagaacga gaaatttgga ttgtttcaga actggaaaat 60 atcaatggcg tgaccgatgg ttacttaaat aggtaaayag tcgccgtgac cttagacgtt 120 tggcttttct caagcttcct tttgccagac ttcatttcct ttatgtgctg gcgttgcctg 180 tggtttgtct tttccttaga ggctcactct cattctcttg ggcttctgag ggagggtcct 240 gcagcctcga tcgtgcccat cctwctgttc craaagccac tgtctcttcc agccaaggca 300 gcctctcaag gscagaactt cgaatgccar cagtcatgca gctcggggcc tgg 353 <210> 26 <211> 387 <212> DNA <213> pig <400> 26 tctttggaaa ttatagaatc caacataaaa attaaaaaaa aaacccttcc ttctttgtta 60 ctataagtaa ctcatttaat cctagtttac agttgggaat aaacctaaca gagtagacgt 120 tgtatgatgt acacagtcat ataagctatt attaattata ttgcttattt caactaaaga 180 ttgaaaaatt acataaataa ttctgtatta tagagcagct gaagtctgaa aggagtcacc 240 ctttgttagc accagytgat tttggataga agtagtcact tagattctga cacaacattt 300 gatgcrgatt gtctaaagtg tatatttgtt tttcagctgt aagaaatgtg atcttacaga 360 agtggagctg gataatcaaa tcgttac 387 <210> 27 <211> 462 <212> DNA <213> pig <400> 27 tttccccttc ccctgaggtt tcaccctcct ccccaatttc tccgcacagt kcctggggac 60 ttgcgtctct tccatctagg atagaccttg ccctcaggta acctgggtat tggggccagr 120 tctgtgccta taccccgccc ttcccccagc tagaccaggg agtgaagtgg cccctagtgt 180 ctccacgtgt cctcccctct ctcccagctc tgcacttcca ctctctttca gatctctagc 240 tcctacacaa ccaccmctac catcacagca ccttcctcca gggtcctgca gaatggaggg 300 ggcaagttgg agaagactcc ccaataygtg gaagaagaca tccgccctga aatgaaagat 360 gacatctatg acccaaccta ccaggataag gagggcccaa ggcccaagct tgaatatgtt 420 tggagaaaca tcatcctcat gagtctgcta cacttgggag cc 462 <210> 28 <211> 520 <212> DNA <213> pig <400> 28 cctgctcccg ttgctccatg cagccctcac ttcctccttt tgcttytcca gatcccgcac 60 caggtctcgg aaattctgct ccargacggc cttctcattg gtggtgaagc tctgygtcca 120 gmgtaaatta gagagaggaa ggaggctttc agtaatgaac aaaacctccc tagtaggtgc 180 atctttaggt tcccaacaca gagtcagggc atttctatgc wctcacaccg caagcctgtt 240 caaccaagaa tctcattcaa aggcwacacc gaggcatgcc atctattggt ggagatgggc 300 aggctttttt acagtccacc ttttgttgtc tatctgcagc tcayccccct tggtgggttg 360 acatgaaccc tcaytccatc cttgggcaac tccatgggcc caaaagtttc cagatgagtc 420 aarttctgtt cattgaaacc tcaaccagaa tcraacatca gtcakaaagt aaatggaggg 480 ggtgtttctg tagccagggt caaggggcac tgatctcagg 520 <210> 29 <211> 498 <212> DNA <213> pig <400> 29 ttccagcact tcaatgagtg catcttccac ttcaacagct acgagaagca tgaaaagtac 60 aacagatttc cccagtcgga gaggtcagtg ctgcaggggg tgctgggarg gtggctcagg 120 tacagtgctg tggtgagatg gtccaattct gccctagaga aaagcggcta ttttcgctga 180 aggggaagac aaataaggag aaacggatga aaatctacag tttcctcctg gaacacttca 240 cagatgagca gcggttcaac atcacctcca aaatctgcct cagcacactg ggtaggactg 300 gttggagtgt agggatgaac taggtgagca agcaaactgc tgcctgtctc atggcccagc 360 cactctgcag caaagagggc ccctctgtcc ttggccctca gctggctgtk cgttcccctc 420 aggcagcaat tctcagaagc ccatctctgc ttcctccagc ttgctttgcc gatggcatcc 480 tgcccctgga catggaag 498 <210> 30 <211> 462 <212> DNA <213> pig <400> 30 gacaacatgg gcttagtctt accgcctcgt gtagcctgtg tccaggtaag gaaaatgctt 60 ctgattatca ttgcttttat aaaaatagaa gccattgtga gtaaagaaaa tttcaaagtt 120 taacttactg aatgtcattc aggtagattt tgctgataca gaaacaatat agaatagagt 180 ctagacctca gtattatgta actactrttt gtgtgactgt tttttactct taattttttt 240 acccatttta taaagctttt agaaattatt ttttgaagca gtgtataata aatgaacaaa 300 aatgcattca ttatgracaa acactacgcc gctagttgcc aggtattcwg ygtgggttag 360 aaatcacaca ggttgatttg tgtgarggtt tgtagtcact ggtatttgga ctyctggatg 420 cttaygtggt gatgtgactg cattacttct ctgggctgct tc 462 <210> 31 <211> 472 <212> DNA <213> pig <400> 31 tgatgtggga agcgttttcc tatggacaga agccrtatcg ggtgagctcc cggcgcttcg 60 ctycctaatt acgcagccrt cagaacagat gggcagcgga tgatctttgc aggaycctga 120 caatcakcaa atccggacac tctttctggg tctttatctt ttgaaagtgg acagtaaata 180 taatctcagt ttattctaaa tggaaagaaa catttgmagc tgtctggaca gtatgtgttt 240 ttcattgatg cccacatata aatgggcata aaatatatac ccrcatcaac attaattaga 300 gtggaaatag aaataatggc tatttccatt atcaagtgtg tggctcggtt acttttttca 360 tcaaaatata tatactgaaa aatgcacaga tcctaggata cagcttgatg aattttcaca 420 attgggggac catgaaacca gcccccaaat gaagaaacag aacagaaccc ca 472 <210> 32 <211> 361 <212> DNA <213> pig <400> 32 acaagcaggt gagaccatcc tctcttgaaa gcacaccccc caacccacac ccacacccac 60 ccattccagc ccagagcatc cagaatgctg ccggagccct aactcccctt ctctttccac 120 aagctgacct ggattgcact caaggtatta ggcatgagac ctgggcgtga gcttgtctgt 180 gatgattcag cyttcccgtg tggattagag agagacttgc aacaactgaa cttgggggtg 240 accctcaggg gcttgsatct ctgtccctcc atttcagccc cttcccctcc tccccacccc 300 tgcagtttgc actctataag aagatgaccc aggctgccat cctgatccag agcaagttcg 360 a 361 <210> 33 <211> 485 <212> DNA <213> pig <400> 33 cctgcatcct gagactggcc ctcagactga aggagcccct tctgcggggc ytcccactca 60 caggcgggcg tccccgtacc tacacrtaca gtgacgggga ctggccagca gagggcgccg 120 aaagctcact gtgggacccg cctttgcttt tcaggatggt gagttttttt gttttttcgt 180 tgtgtgtttt tttggtttgc tttttgtttt taacttttat tgaagtatag tcgatttaaa 240 atgtgataat caggayggcg ctttattgtg acttacggag ctgggtggct ttaagcaaat 300 cctttccttt cctgagcctg agmctgacct cggtcccttc catctcagaa wtgtcttcct 360 cccagcaggt tcctccccag gagccacacc tcccgcagca ccaccgcccc scacctggtg 420 ccrggggacc acgtacccgg ctggaggtga agtctcgggt ctttgcgcga agcttattga 480 cctga 485 <210> 34 <211> 568 <212> DNA <213> pig <400> 34 catcatccct ccattcctgg cctatggcga gaaaggctat ggtgagggca ggcagggacy 60 tggggattct ctggaagtgg gctgcacaca gttgttcagg ttgtgtactg cacaaggaca 120 tctagccaag gaggcaagta gggacgaaat ctagcccttg gtgatgactt ccctaagaag 180 aggtgcttta tcctgatkca cacaaaggct cagtatgggc tggtggtggc tcccgggaaa 240 aactcagctt aggtctcgca agggaagaac caacyggggc tgctgatgag caggaaagcg 300 ctctggaaac cagggttggc gcctttttgy gtgatgctca cttacgcctc cctgagagcc 360 ctgttttgct ctccacttgt atggttcaag cctatccctt ccccagggac tgtgatcccc 420 ccgcaggcct ccctcgtctt ccatgtccta ctgattgacg tccacaaccc caaggacact 480 gtccagctgg agacgctgga gctgccacct ggctgcgttc ggagggccgt ggctggggac 540 ttcatgcgtt accactacaa cggctccc 568 <210> 35 <211> 426 <212> DNA <213> pig <400> 35 acattattta gaaagtgaag cctgaaattc ggctgctgat cgagaaatgc aacacggtga 60 ggcaggggct gatgacctag gggctgaccc caaatagcct ggcttgagct atgcaagaca 120 atggatgtgg ggccagaggc gtggaggtga tgttggagtt ggcagactaa wtatcttgac 180 tcctgtgctc ctggcatctg gccccagcag catgagttgt atatgtcttg gcttaagtag 240 aaaaggctaa gatcctcata tgcatatttt ggtcccctca cccttcaggt caaaatgtgg 300 gtacaactcc tgattcctag gatagaagat gggaacaact ttggggtgtc cattcaggta 360 atgtgcttgc attggaaatg ggagaggaag gtttgggagg taatctcatc tcccctgctt 420 ttgtgt 426 <210> 36 <211> 532 <212> DNA <213> pig <400> 36 ctgctggctg gagaacggac tcctccaact ataaccccgt ggagatgtgg aactctggct 60 gccagattgg tacgggctgg cygagacggg gaggaggcct gggtgayggt ttctgcctgg 120 agggccaggg gccaggagcc tctggggacc tgggcagagc cagatacagg gatttgtggg 180 ctcaggatct ggcagtctgt ttggaaatat cccccagcag cacatggcag catmtgaaat 240 ctgtcatcct ttgaatcctg gcaccagaaa tgcatgggyg ttggtgtggt gggtcaggga 300 gggtggcagg tgggtatcag gcatcagagt gatcctggac cctgcttttg cttaggactc 360 tgtaccccca gtatttcwwa gggcaaccca cagcctctga ttgctctgtc ctgcccttag 420 tggccctgaa cttccagaca cctgggccag agatggacgt gtaccagggc ckcttccagg 480 acaacggggc ctgtgggtat gtgctgaagc ctgccttcct gcgagacccc aa 532 <210> 37 <211> 429 <212> DNA <213> pig <400> 37 cagtcgtggc agagtgagcg ggagatcttc agcacgcccg gcatgaagca cgagaacctg 60 ctacagttca ttgccgctga gaagcgaggc tccaacctgg aggtggagct gtggctcatc 120 acggccttcc acgacaaggt gagccatgct tgtggctccc gctcccacgt aggtgaacag 180 gccctcctgt ggctttccat ggctgtggat gtgaaggtgt gcagtcaraa accctamccc 240 tgggatcact tcttaggaat agtaatgact tcatgatcac caccccctac agcgcctttc 300 caaccccttg tggcctcatc kcttccagcc tatagcccac agagacagcc ctcctgggct 360 gggctgggct gggttctgtc ctgtgcccag agcctgtgct gtctcctcta gggctccctc 420 acagattac 429 <210> 38 <211> 272 <212> DNA <213> pig <400> 38 ctccaaccaa caagctcctc tatgccaagg agattcctga gtaccggaaa atcgtgcagc 60 gctactacaa acagatccat gacatgaccc cactcagtga gcaggagatg aatgcgcacc 120 tggctgagga gtcgagggtg cggccggctc tgaggggacc ttgcctggtg ggggatcccg 180 aggaccagtg gtggcagcga ggggctgtgt ctaaggagca gccatggctg ggctggccrg 240 gtgctgaccg tggagttaac ccccttccca gc 272 <210> 39 <211> 390 <212> DNA <213> pig <400> 39 cgaggcggct ctcgcccgcg ccgagctcga aaagcgcatt gacagtctga tggacgaaat 60 ygcctttctg aagaaagtgc acgaagagga gatcgccgag ctgcaggckc agatccagta 120 cgcgcagatc tccgtggaga tggatgtgtc ctccaagccc gacctctccg ccgcgctcaa 180 ggacatccgc gcccagtatg agaagctggc cgccaagaac atgcagaacg ccgaagagtg 240 gttcaagagc cgcttyacgg tgctgaccga gagcgccgcc aagaacaccg atgcggtgcg 300 cgcagccaag gacgaggtgt ccgagagccg ccgactgctc aaggccaaga ccctggagat 360 cgaagcctgc cggggcatga acgaagcgct 390 <210> 40 <211> 506 <212> DNA <213> pig <400> 40 gtgcagagct gattgagcag ccgctccgca tcagcctcgg ggacgcccag ctctatgcgc 60 ccaatgcccc gctcagcggg ccggtgctgg ccctcatcct caacatcctc aaaggtgagc 120 gccctccgca gccctgcaga gcagtgatct gggtgtggct gctcttcaag gtctgggtgc 180 cacagtgttg gccagagttc actgctgagt ggcctggtcc agagggcaca tctgggaaac 240 ctcagtgstc mtccgagagc atgtggacca gaagccctgt ggctggacct gtgaggccca 300 tggcttcctt ccagccatca gtgcttccaa agcagctgct gccgttgggg attaaaaggt 360 ccccatctca aggtagccaa gtggcccagg gaggggtcaa ccacaccccc agggcactca 420 gctgtctggt caagctggga catccagggc caccaacctg cctggctctg tcaatcaggg 480 tacaacttct cccgggcaag tgtgga 506 <210> 41 <211> 511 <212> DNA <213> pig <400> 41 tttaccttat caaagtacat caagttcctc ctccatgaac ccattgtcaa caagttccgg 60 gaatacaagg taaggcctcg ctctacggtc tacatgagtt ccttggcagc tctctgtata 120 cctgctaatg cttaccttgg gcatattgat ctctctgaga caaggactac aaagggtgag 180 gcaattctgc ctcagaattg tggcggcggt ggggtggagg attgacaaag atctcaggta 240 tggaaatcat ttatayagaa cctggccagt gattaatgtt ttacctgtca ttcttttccc 300 ctggttcttt tggaaaagag gaaaattact gcctctgaag caaagattgt ggtctttcag 360 ggatgctctg gtgtgttttc tgcccaaaga aattgcttcc attagcgttc tttctggaat 420 tgctcccaag agataaggct ttccatccca caaatagaaa ctgttaaatg cttaaatacc 480 acagggaaag taaaaaccta aatatagcca a 511 <210> 42 <211> 470 <212> DNA <213> pig <400> 42 gaattcagaa aagaactttc caatggacat gcctgatgat tatgagactc agcagtggcc 60 agaaaggaag ctcaagcaca tgcgtttccc tcctatgtat gaagagaatt ccagagacaa 120 cccctttaaa cgcacaaatg aaatcgtgga ggaacagtat actcctcaaa gtcttgctac 180 attggaatct gtcttccaag agctggggaa actgrcagga cccaacaacc agaagcgtga 240 gagggttgat gaggagcaaa aactttacac agatgatgaa gatgatatyt acaaggccaa 300 taacattgcc tatgaagatg tggttggggg agaagattgg aacccagtag aggaaaaaat 360 agagagtcaa acccaggaag aggtaagaga cagcaaggag aatatagaaa aaaatgaaca 420 aatcaatgat gaaatgaagc gttcagggca gatgggtctc caagatgaag 470 <210> 43 <211> 439 <212> DNA <213> pig <400> 43 gacagccaga gattcaatcc ttagacacaa gattctgggt aatgagagcg aagaggaatt 60 tctaactgga atagagaatt tggaaatcag agaaacagaa accattaatc cccttgatgt 120 cctgtgtgcc tctatgctgt gttcagacag ctctttgcaa cgcgaagtca tgtcaaacat 180 gtatcaatgc cagtttgctc ttcccctgct actgccagat gcagaaaaca acaagagcat 240 tttaatgctg ggtgcyatga aggacattrt gaaggagcag tcaacacagc cttcaggagg 300 tcctacagag ggtacggaaa agcttctgac tctcatgaag atgcccgtca tctcttttgt 360 gcgtctagga tactgtagct tctccaagtc cagaatcctc aacacactgc tcagccctgc 420 ccacctgaaa tcacacaaa 439 <210> 44 <211> 449 <212> DNA <213> pig <400> 44 gttcaccaag aaggagctgc agtccctcta caggggcttc aagaatgtga gtgctcccca 60 ttcccctggg agaggacttt gttccccagg ccctcttgtc cttggmccca ggagggaggg 120 actctgatgc aggaggacct tctkgaaatt ttccatggca tggtagatat cccagggcct 180 ttatggggct cacrttttgt gagagttcat gttctagggg gtgggctgcc ccagaatcag 240 tgttctgaag gccccgtgct ccctgctgtg tgtgggagga acacgcagtk ggatccagct 300 tcccgccagg cccaccyggg gctggtagag cccagtggtg cctcagccag caggcgtccc 360 ccacggggag gagagaggcc agccctgtgg ctcggggtgc ggggcggggt ggctggggtc 420 actgagaggg agaccttcct gctgcagga 449 <210> 45 <211> 481 <212> DNA <213> pig <400> 45 gtcycccgtc acacygcctg rcccccgcac ccccaggttc tgtacccccg agggctgggc 60 gggcagactc cggagcacag gagtcgggct ccctggggag ggaaaggggg gtgctgtctc 120 tgccccctcc ccgctcccca ccttatgcag gaatccctag tgacagcaaa agcaaagggc 180 aggcgggcca ggagctcagc aagctccagg ccaacccagg ccaagcgaat tgggttacag 240 tggcctcccg cagatyccat gtgatgggga aacgcctcct gtggggatcc aataactgtc 300 ccccagctcc cccgcccaga cgctcactgc ctcgcaagcr cttccaytct ggggtctgca 360 ggcrctgtgc tcaccccctc cccctygccc cttccagaca ctcaagaacg cctggctgca 420 gcgctacgca gtgaacagcg cagaccccgg cgtgtttgtg ctcctggcct gtggcaccat 480 g 481 <210> 46 <211> 313 <212> DNA <213> pig <400> 46 tattcccatc atcaaactgg aatggcattc cccatgggct gtcatccctg tcttcctygc 60 aatgttgggc atcatcgcca ctatatttgt catggccact ttcatccgct acaatgacac 120 tcccatcgtc cgggcatctg ggagggaact gagctatgtt ctgttaactg gcatctttct 180 ctgctacatc atcactttct tgatgatagc caaacctgat gtggcagtgt gttctttccg 240 gagagttttc ttgggcttgg gaatgtgcat cagttatgca gccctcttga caaaaaccaa 300 tcggatttat cgc 313 <210> 47 <211> 385 <212> DNA <213> pig <400> 47 ggccttcaat gtgagcgccc gggggacgcg cgggcggggc gggccgcagc cgggccagcc 60 cttcccacag cctgtgtctc ccgcagatgt ttgtgcgtct ctttgtggat gataacttgg 120 accgcatggk gcccatctcc aagcagccca aggagaagat ccaggccatc attgagtcgt 180 gcagccggca gttccccgag ttccaggagc gggcccgcaa gcgcatccgc acgtacctca 240 agtcctgccg gcgcatgaag aaraatggca tggagatggt gagccccgag tcctggcccg 300 ctgcccctgt tccactctgt gctctgctcc ggggctcccc gacatcctcc cagcgggggc 360 gcttcgccca cagccctcct agtcc 385 <210> 48 <211> 608 <212> DNA <213> pig <400> 48 cggcctgtac cccagggatc cccctgccta gccstttgta ggggatggtg tcgggccctc 60 cctccccatc tggtgctcct gtggcttctg gaagggccac tttttcttcc cccggagggc 120 aggctcagcc tmccgcaggc ctggccctca caccaggtcg aggagcctct ggcatctcgg 180 gctyggccct gcctttgggt tcccctctgg gggtgaccag ggttccatag gtgtggctgg 240 cctcagaggt gcctttgggc ctggcctgtc tttccagtgc tcctcctgcg cccagcagtt 300 catgcagaag aaggacttgc agagccatat gatcaagctg cacggagccc ccaagcccca 360 cgctgtaagt gccaggccgg gctgagcatc ggggccggcg tgttcccagc aagagtgagg 420 cggctgtgrc tgcgtctaca atgggtgggc tgtgaggttg ccaccaggtg gcaggggcgg 480 ccrggggggt tcccgggggc cctctggggc cctagggaca gcccttcctg ccctttactc 540 tggccctgct ccctctgccc cttagtgtcc cacttgtgcc aagtgcttct gtcccgaacg 600 gaactgca 608 <210> 49 <211> 465 <212> DNA <213> pig <400> 49 ccgctggcgc ccgacgggat cctggtgttc agcggaggcc agagtgggcc tgtggaggac 60 tttgtgtccc tggcgatggt tggcggccat ctggagttcc gttacgagtt gggatcaggt 120 gagcactgcc rccaaatgca gatgggcata ggcactaagc cctgtgcccc raggaggcgc 180 gaaggagtga attctgaagt cagaccaact ggagtccccg ttcttcgttg cttctctctg 240 tgtgactgga agcaagtccc tggatctctc aggttcctct gggacascct agacaggaag 300 gaagttcccc atctgctcaa gttccaggag ggggtgggac agggcagtga ccatccctgt 360 gccaaccctg ccgggcatcc tccccagggc tggccattct gcggagctcc gagccactgg 420 ccctgggccg ctggcaccac gtgtccgcag agcgtttcaa caaga 465 <210> 50 <211> 376 <212> DNA <213> pig <400> 50 gctggcacgc ggctctcacg tggacgtcct gtgggccccg ctttcaatga cgggtttctt 60 tcccagggat ggtgaagcac attacaggca gttacaaggt tacctaccay ccagatggcc 120 cagagggtca agcctatgag attgacttca cgcccccgtt ccggaaaatc agcatggtgg 180 aagagcttga gaaagccctg ggaatgaagc tgccggacac taacctcttt gaaacggaag 240 gtaaagtgat gcgygygctt ctctgctgta gggcctgcct tcctttkcag ctctgagtta 300 gatgaggtcg ggactagaat gtcccctgct cttctctttg tgtacttttt aaggggtggt 360 atgatgtctt ctgtcc 376 <210> 51 <211> 484 <212> DNA <213> pig <400> 51 cctcttcctc atcacaggcg gctccctcag ggagcacccg gtgttgggag gccaggaagt 60 tgaacatgtc aaaggtgcac ttcctgcagg ggaaggaagg aaaggaggga gggtcagagc 120 cattctttta agaagatgct ccccctccat tctttggcct gggcctgccr ctggyggcgt 180 aagaagaata ctgcttctac tgaagacaca gcaaaagggg aacagatggg attcattctt 240 gtaacgtgaa ctctagaaat aatttctgga gcagtcgagt gtcttctccc tagacctccg 300 tttcctttaa ttcccaccct gagccaggca ccacacccca gacctctcac cggagataga 360 cctcagcgcg ggctgcmcca tggggattca ggggtggttc ctcttggccc tctccctgct 420 ggtggtagcg gaacttatag tgctggcagc gctgggctcc aggcagctgc tctgccagga 480 agat 484  

Claims (2)

264 nucleotides of SEQ ID NO: 1, 287 nucleotides of SEQ ID NO: 2, 496 nucleotides of SEQ ID NO: 3, 219 nucleotides of SEQ ID NO: 4, 256 nucleotides of SEQ ID NO: 5, 290 nucleotides of SEQ ID NO: 6, 267 nucleotides of SEQ ID NO: 7, 256 nucleotides of SEQ ID NO: 8, 370 nucleotides of SEQ ID NO: 9, 117 nucleotides of SEQ ID NO: 10, 171 nucleotides of SEQ ID NO: 11, 143 nucleotides of SEQ ID NO: 12, 196 nucleotides of SEQ ID NO: 13, 295 nucleotides of SEQ ID NO: 14, 228 nucleotides of SEQ ID NO: 15, 143 nucleotide of SEQ ID NO: 16 sequence, 131 nucleotides of SEQ ID NO: 17 sequence, 423 nucleotides of SEQ ID NO: 18 sequence, 256 nucleotides of SEQ ID NO: 19 sequence, 230 nucleotides of SEQ ID NO: 20 sequence, 247 nucleotides of SEQ ID NO: 21 sequence, 225 nucleotides of SEQ ID NO: 22, 384 nucleotides of SEQ ID NO: 23, 431 nucleotides of SEQ ID NO: 24, 272 nucleotides of SEQ ID NO: 25, 306 nucleotides of SEQ ID NO: 26, 327 nucleotides of SEQ ID NO: 27, 344 nucleotides of SEQ ID NO: 28, 109 nucleotides of SEQ ID NO: 29, 349 nucleotides of SEQ ID NO: 30, 115 nucleotides of SEQ ID NO: 31, 256th nucleo of SEQ ID NO: 32 sequence Tide, the 411th nucleotide of SEQ ID NO: 33 sequence, the 198 nucleotide of SEQ ID NO: 34 sequence, the 171th nucleotide of SEQ ID NO: 35 sequence, the 378 nucleotide of SEQ ID NO: 36 sequence, the 237th of SEQ ID NO: 37 sequence Nucleotide, 239th nucleotide of SEQ ID NO: 38 sequence, 61 nucleotide of SEQ ID NO: 39 sequence, 251 nucleotide of SEQ ID NO: 40 sequence, 256 nucleotide of SEQ ID NO: 41 sequence, 215th of SEQ ID NO: 42 sequence Nucleotide, 269 nucleotides of SEQ ID NO: 43 sequence, 290 nucleotides of SEQ ID NO: 44 sequence, 256 nucleotides of SEQ ID NO: 45 sequence, 58 nucleotides of SEQ ID NO: 46 sequence, 263th of SEQ ID NO: 47 sequence Nucleotide, the 184th nucleus of SEQ ID NO: 48 sequence Consisting of 8-100 consecutive nucleotides comprising a leotard, the 171th nucleotide of SEQ ID NO: 49 sequence, the 287 nucleotide of SEQ ID NO: 50 sequence, or the 175th nucleotide of SEQ ID NO: 51 sequence, to determine the origin of pork Useful polynucleotides or their complementary polynucleotides. How to determine the origin of pork, including the following steps: (a) isolating nucleic acid molecules from pork to be traced to origin; (b) identifying the base type of the marker of Single Nucleotide Polymorphism (SNP) corresponding to the nucleotide of the designated position of SEQ ID NO: 1 to 51 sequence described in paragraph 1 above and storing it in the database Doing; And (c) Analyzing the origin of the pork using the markers of the parents already stored.