KR20110026704A - Method for manufacturing fish liquefied fertilizer from marine wastes - Google Patents

Abstract

PURPOSE: A liquid fish fertilizer based on aquatic waste and a method for forming the same are provided to mass-produce the fish liquid fertilizer by biologically and physically aquatic waste from fish. CONSTITUTION: A method for forming liquid fish fertilizer includes the following: aqueous waste is sterilized at low temperature and is pulverized. The pulverized aqueous waste is rapidly proteolysed based on aerobes. Particle fragmentation is processed based on underwater vortex. The aqueous waste is aged and stabilized based on anaerobes. Uniform liquid fertilizer is obtained.

Description

Fish liquid fertilizer using fishery waste as raw material and its manufacturing method {Method for manufacturing Fish Liquefied Fertilizer from Marine wastes}

The present invention relates to a fish liquid fertilizer using fish as a raw material of the marine waste and a method of manufacturing the same, and more particularly, to sterilization of aquatic waste consisting of fish head and internal organs separated and discharged during processing, sterilization, aerobic decomposition. It is a liquid substance obtained by processing the process, the fine grinding process, the anaerobic process, and the separate packaging process step by step. The preparation is free from secondary pollution to the environment during distribution or fertilization process, and it is relatively simple to process compared to the chemical preparation method. It relates to a method for producing a liquid liquid fertilizer containing a uniform and uniform fish amino acid.

Traditionally, coastal folks have long known fish amino acid production methods using fish and their wastes, and have been used for farming to broaden crops and improve fruit sugar.

Recently, as interest in eco-friendly and organic farming and organic products has increased, there has been an increasing interest and demand for organic fertilizers, which can replace conventionally used chemical fertilizers. ,

Fish amino acids are of greater interest, and in areas where fish waste is generated in large quantities, each has its own facilities and tries to produce them.

There are many methods and techniques for producing fish wastes from water-soluble fish amino acids, that is, liquid fertilizers. Representative methods include thermochemical production methods of hydrolysis with proteolytic enzymes and biological degradation production methods using microorganisms.

Recently, as the interest of eco-friendly and organic farm products has increased, eco-friendly materials with high organic content are being promoted as a fertilizer to reduce and replace the use of chemical fertilizers, which show various harmful effects, and contain fish amino acids that can be used as eco-friendly liquid fertilizers. Fish liquid fertilizers are getting more attention as organic materials made of natural products that can satisfy these conditions, and in the region where a large amount of marine waste is generated, they have facilities or produce them in their own way.

There are many techniques for producing fishery waste from water-soluble fish amino acids, ie, liquid liquid fertilizers. Representative methods are thermochemical production methods that hydrolyze with proteolytic enzymes and biological degradation production methods using microorganisms.

*. Thermochemical manufacturing method that hydrolyzes with protease

Published Patent No. 2002-0006548 is a patent describing amino acid liquid fertilizer or fusion fertilizer through low-cost thermochemical proteolysis prepared using animal blood, fish carcasses or fish by-products,

Publication No. 10-2004-0065201 relates to a method for producing an amino acid liquid fertilizer using an inexpensive and efficient protein hydrolysis and strong acid additive, which is manufactured using animal blood and carcasses, livestock processing, fish carcasses or fish by-products. In order to hydrolyze the protein of the organic material of the organic matter, a strong acid such as nitric acid or sulfuric acid is added together with chymotrypsin, which is a protease, and water, followed by heating and pressing to liquefy the workpiece.

Biodegradation method using microorganisms

A simple processing method for producing a marine anaerobic waste by mixing and sealing a small amount of indigenous microorganisms or complex microorganisms consisting of lactic acid bacteria, yeast, photosynthetic bacteria, and molasses for a long time anaerobic process;

As related to Bacillus subtilis strains 18-1 and 19-2, which are capable of decomposing a hydrophobic waste into a water-soluble amino acid as disclosed in Japanese Patent Application No. 2000-0007270, and particularly suitable for treating fish residue and fish oil,

The method relates to a method for preparing a liquid fertilizer using a fish waste waste solution, such as Patent 10-0822239 and Patent 10-0855338, wherein the fish waste waste solution is Bacillus tilis, Bacillus rickeniformis, Bacillus agri, Bacillus coagulans, Bacillus circulan To produce liquid fertilizers in a short period of time through the aerobic process using Bacillus, such as a method for producing a liquid fertilizer using fish pulmonary fluid, characterized in that it is decomposed into a fish pulmonary fluid decomposition bacteria consisting of Bacillus anrasis and Bacillus push form There is a way.

Until now, anaerobic methods of biodegradation methods are not economical because they are manufactured over a long period of time, and if they are not liquefied, such as fish waste, aerobic methods can produce liquid fertilizers in a short time. In order to produce a lot of residues such as scales and gills and require secondary treatment or to produce a large amount of liquid fertilizer uniformly and stably, the intermediate treatment process may be complicated or expensive equipment may be used.

Accordingly, the present invention minimizes the risk of secondary pollution due to residues by eliminating any emissions or exports other than the resource cargo produced as a product in the process of treating fishery waste, and manufacturing eco-friendly fish liquid fertilizer in a uniform and stable manner. I would like to present.

The main object of the present invention is to provide a method for producing a uniform and stable eco-friendly fish liquid fertilizer by fast and simple treatment by treating a large amount of fishery waste generated in a fish wholesale market or a large distribution center by predetermined process steps. .

Therefore, an object of the present invention is to produce a large amount of fish liquid fertilizer containing a high quality fish amino acid in a fast and simple process.

Another object of the present invention is to provide a method for producing an environmentally friendly fish liquid fertilizer to reduce the pollution and reduce the pollution of the input amount to the entire amount of the input material as a fertilizer so that there is no residue of the existing manufacturing method.

First, the present invention is to process the marine wastes step by step by classifying the physical methods such as heat treatment, crushing, underwater vortex and biological methods through the anaerobic and anaerobic microorganisms,

A process of pasteurizing the marine waste by pasteurization in a first step;

Fast protein degradation by aerobic microorganisms in a second process;

Particle segmentation by vortices in water in a third process;

Fermentation and stabilization by anaerobic microorganisms as a fourth process;

Obtaining a homogeneous liquid fertilizer in a fifth step;

The sixth step relates to a method for producing a fish liquid fertilizer in which the fish liquid fertilizer commercialization step is processed step by step.

Secondly, the present invention minimizes the emission of secondary pollutants for the purpose of recycling the oil produced by the three-phase separation in the fifth process as a separate fish oil, and the remaining sludge is returned to the third process to reprocess. It relates to a manufacturing method of environmentally friendly fish liquid fertilizer.

Fish liquid fertilizer according to the present invention contains a sufficient amount of homogeneous amino acids and organic acids, enabling economic mass production, thereby reducing the cost of farming materials for farmers growing eco-friendly organic crops, improving the price competitiveness and quality of crops Can contribute to In addition, since the recycling of fishery wastes increases the yield of recycling resources and minimizes secondary pollution, there is a great advantage not only in terms of waste resource utilization and economic aspects but also in terms of environment.

Hereinafter, the present invention will be described in detail.

When listed step by step the process for producing a liquid liquid fertilizer in accordance with the present invention.

(1) The first step: The first step is to recover the head and viscera of fish separated into inedible parts that are not suitable for food in a fresh state without decay, to remove harmful bacteria causing rancidity and polymerize at the same time. It is a process to expand the contact area of microorganisms and to make biodegradation easier by disintegration after sterilization at 60 ~ 80 ℃ for 1 hour, which is considered sufficient for sterilization, for the purpose of loosening and decomposing the hard meat protein.

(2) Second step: In the second step, incubate the aerobic microorganisms with 0.1 to 0.5% by weight of the crushed fish after mixing 20-50% by weight of purified water (water) with the pasteurized and crushed crushed fish. In the early stage of fermentation promoting liquid, molasses, which is a natural product, is added to 5 to 20% by weight of crushed fish meat. At the same time, it is an aerobic decomposition process for 12 to 24 hours to achieve the purpose of stirring to prevent precipitation.

① molasses is a by-product of sucrose manufacturing process, there is no particular limitation on the type of molasses used in the present invention, any one of sugar cane molasses or sugar beet molasses, waste molasses, refined molasses, ice molasses, Any edible molasses can be used.

② On the other hand, microorganisms used in aerobic digestion are Bacillus subtilis , Bacillus Iicheniformis and Bacillus coagulans which are lactic acid producing bacteria. Actinomycetes Streptomyces griseus are used to prevent early rot.

(3) Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans are preferably used in combination of 1: 1: 1, and Streptomyces griceus at a concentration of 1-3% with respect to all microorganisms.

On the other hand, the total number of microorganisms of the total microorganisms is preferably 1 X 10 ⁷ cfu / ml or more.

(3) Third process: The third process is a process to more finely split the residues which are not decomposed by microorganisms in the second process, and aerospace for fertilizing tank is partitioned by aeration for oxygen supply, and corrosive and hard rock granite This is a process for further subdividing the particles of fine fish by arranging the crossroads and passing strong vortices of high velocity generated through the axial stirrer for 36 to 48 hours continuously.

(4) 4th process: 4th process adds microorganisms required for anaerobic process to 0.1 ~ 3% by weight of the initial fishery waste in the intermediate composition solution in which a significant amount of sludge is reduced and matures for 72 hours at 30 ° C. in complete anaerobic condition. to be.

As the microorganism used in anaerobic process, it is preferable to mix lactic acid bacteria, yeast and photosynthetic bacteria at a concentration ratio of 1 to 2: 1 to 2: 4 to 8, and actinomycetes to 1 to 3% of the total microorganism. It is more preferable to mix lactic acid bacteria, yeast and photosynthetic bacteria at a concentration ratio of 1.5: 1.5: 7 and actinomycetes at a concentration of 0.1 to 2% with respect to all microorganisms.

More specifically, the lactic acid bacteria used in the anaerobic process is used in a mixture of two or more of Lactobacillus ashidophyllus, Lactobacillus plantarum, Lactobacillus casei or Lactobacillus rhamnosus 1: 1 ratio, in particular Lactobacillus Preference is given to using Ashdophilus (KCTC 3164), Lactobacillus plantarum (KCTC 3108), Lactobacillus casei (KCTC 2180), and Lactobacillus rhamnosus M21 (KCTC 10965BP).

Yeast may also be used in Saccharomyces cerevisiae, in particular Saccharomyces cerevisiae (KCTC 7286), and as a photosynthetic bacterium, Rhodoschodomonas capellata, in particular Rhodoshudomonas capellata (KCTC 2583), Actinomycetes may be used Streptomyces gliseus, particularly Streptomyces gliseus (KCTC 1072).

On the other hand, the total number of microorganisms of the total mixed microorganism is preferably 1 X 10 ⁷ cfu / ml or more.

(5) 5th process: 5th process is anaerobic process, using a three-phase separator from the aging liquid stabilized with amino acids to separate the micron solids into undigested fats and undegraded sludge, and to remove fish liquid fertilizer. In the extraction process, the oil-fat separated from oily oil is purified by distillation process to remove water and provided as a fish oil semi-finished product for feed or industrial use.The separated sludge is returned to the third process for re-decomposition and homogeneous fish liquid Fertilizer production process.

(6) 6th process: 6th process is the final packaging process. The final finishing process is to dilute or concentrate the total amino acid content to meet the labeling content of the registered product according to the provisions of the fertilizer's process standard without sedimentation. to be.

Hereinafter, the present invention will be described in more detail with reference to Examples, which are intended to aid the understanding of the present invention but are not intended to limit the scope of the present invention in any way.

Example 1 Preparation of Fish Liquid Fertilizer

In the first process, 2 Kg of fish head and 2 Kg of mackerel waste are put in a prepared heat reactor, and sterilized at 80 ° C for 1 hour, and then crushed in a state where only the form is lost by using a blender to make bulk sterilized crushed fish meat. .

In the second step, the crushed fish meat and water made in the first process are mixed in a weight ratio of 1: 1, and the aerobic microorganism is added at 0.5% by weight of the crushed fish, molasses is added 20% by weight of the crushed fish, and the internal temperature is 40. The crushed fish meat was decomposed while stirring at 150-200 rpm for 24 hours at ℃. Meanwhile, air supply is omitted.

The aerobic microorganism used herein is a mixture of Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans in a concentration ratio of 1: 1: 1 and Streptomyces griceus in a concentration of 2% by weight based on the total microorganisms. . In addition, the total number of microorganisms used is preferably 1 X 10 ⁷ cfu / ml or more.

After 24 hours, about 8L of the result (Fig. 2) was obtained, and it was estimated to be partially steamed by CO ₂ and heat as a fermentation gas because it was decomposed with stirring at an internal temperature of 40 ° C.

In order to further refine the unresolved residue of the result obtained in the second process to the third process,

At the bottom of the 20 liter barrel, the bubble and submersible pump are installed so that the flow direction of water flows upwards, and the coarse granite gravel and the corrosive gravel having a particle size of 5 to 10 cm are placed in the bag. It was set to about 5cm to prepare a microparticle decomposition device by underwater vortex. The schematic is as shown in FIG. 4.

The amount of gravel in the granite and humus is not important here, but the area and length of the water and the residues will be important.

On the other hand, the mixture of 8L and 8L of the water of the second process was mixed in a 20-liter cylinder prepared by a microparticle decomposition device and vortexed under water continuously for 48 hours to obtain a result of about 16L (Fig. 3).

In the fourth step, the resultant was added to a 20L barrel, and the anaerobic microorganisms were added at 0.3% by weight of the shredded fish from the first step, mixed, sealed, and aged at 30 ° C. for 72 hours. .

Here, lactic acid bacteria among anaerobic microorganisms are prepared with Lactobacillus ashdophyllus (KCTC 3164) and Lactobacillus rhamnosus M21 (KCTC 10965BP) in a ratio of 1: 1, which is the yeast Saccharomyces cerevisiae (KCTC). 7286) and the photosynthetic bacterium Rhodoshudomonas encapsulata (KCTC 2583) and lactic acid bacteria: yeast: photosynthetic bacteria were mixed at a concentration ratio of 1.5: 1.5: 7, and the actinomycetes Streptomyces griceus (KCTC 1072) were finally added to the whole microorganism. It is prepared by blending at a concentration of 2% relative to.

Since this embodiment is a simple experiment, the fifth and sixth steps are omitted below.

Experimental Example 1: Component Analysis of the Liquid Liquid Fertilizer Prepared in Example 1

In this experiment, the components of the fish liquid fertilizer prepared in Example 1 were analyzed by the Science and Technology Analysis Center.

As a result of analysis, the amino acid content of the liquid liquid fertilizer prepared in Example 1 is shown in Table 1.

Experimental Example 2 Comparison of Growth of Fish Liquid Fertilizer Prepared in Example 1 and Various Amino Acid Products

In this experiment, the fish liquid fertilizer prepared in Example 1 (hereinafter referred to as "fish amino acid"), gelatin decomposed amino acid (hereinafter referred to as "giyoung amino acid"), gelatin decomposed amino acid and fertilizer (NPK: 2-4-8) Cabbage seedlings were treated with a mixture (hereinafter referred to as "superaminogen") to analyze grass height, leaves, live weight and dry weight.

The experimental group was treated with untreated, macroyoung amino acid, superaminogen and fish amino acid. In addition, the spray method was sprayed 500 times dilution to 72 trays at weekly intervals, the spraying time was observed while spraying at intervals of irrigation, 11 days, 18 days, 25 days immediately after sowing.

As a result of the experiment, the symptom of the spleen (lack of nutrients) occurred only in the untreated group (water supply only), and the rest of the test groups did not develop any abnormal symptoms. The growth of superaminogen was the best, and the growth of the untreated group was the lowest. The area of Chinese cabbage was in the order of superaminogen〉 bigyoung = fish amino acid〉 untreated, and the color of green cabbage was superaminogen〉 huge young〉 fish amino acid〉 untreated. The rooting state of showed a similar tendency (Fig. 5, Fig. 6).

1 is a step-by-step process of manufacturing a fish liquid fertilizer using fishery waste as a raw material.

Figure 2 is a photograph of the result generated after the second process in Example 1.

Figure 3 is a photograph of the result generated after the fourth process in Example 1.

Figure 4 is a schematic diagram of a microparticle decomposition device consisting of (1) submersible pump, (2) bubbler, (3) corrosive bag, (4) granite bag

5 to 6 is a growth photograph of the liquid fertilizer prepared in Example 1 and commercially available amino acid products of Experimental Example 2

Claims (6)

In the method of manufacturing fish liquid fertilizer by processing fish waste such as fish, fish head, internal organs, etc. generated after processing, The manufacturing process, the first step of the seafood waste pasteurization and shredding process; Fast protein degradation by aerobic microorganisms in a second process; Particle segmentation by vortices in water in a third process; Fermentation and stabilization by anaerobic microorganisms as a fourth process; After the homogeneous liquid fertilizer is obtained in the fifth step, the residue is returned to the third step for reprocessing; The manufacturing method, characterized in that the sixth step to prepare the liquid liquid fertilizer commercialization process. The method of claim 1, The manufacturing method characterized in that it includes both a physical process such as sterilization, crushing, underwater vortex and biological processes using microorganisms in the manufacturing process. The method according to claim 1 or 2, In the biological process of the manufacturing process, the manufacturing method characterized in that it includes both aerobic treatment process using aerobic microorganisms and anaerobic treatment process using anaerobic microorganisms The method according to claim 1, 2 or 3, Actinomycetes are added to the aerobic microorganisms and anaerobic microorganisms added during the second step and the third step of the manufacturing process, 0.1 to 2% by weight of the total added microorganisms, respectively. The method according to claim 1 or 2, It is characterized in that it comprises a step of further subdividing the residue into fine particles without damage by mechanical friction by artificially colliding the residue remaining after the protein decomposition by the microorganism in the manufacturing process using water vortex together with water Process for producing fish liquid fertilizer The method of claim 1, The eco-friendly manufacturing method of the fish liquid fertilizer characterized in that the secondary contamination is minimized by returning the residue after the fifth step of the manufacturing process to the third step and reprocessed.

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