KR20100137907A - Single nucleotide polymorphism marker for personal identification and its use - Google Patents

Abstract

PURPOSE: A polynucleotide marker containing SNP associated to individual identification is provided. CONSTITUTION: A polynucleotide marker for identifying individual contains polynucleotide consisting of 10 serial nucleotides with 101th base having SNP in a polynucleotide selected from nucleotide sequences of sequence numbers 1-92 or complementary polynucleotide thereof. Each individual is Korean, Japanese in Tokyo, Chinese in Beijing or Utah inhabitant derived from ancestor of North Europe and West Europe. A microarray for identifying individual contains the polynucleotide marker.

Description

Single nucleotide polymorphism marker for personal identification and its use}

The present invention relates to a single nucleotide polymorphism (SNP) marker for personal identification and its use.

Personal identification using short tandem repeat (STR) gene marker analysis, which has been established over the last decade through the development of gene recognition techniques and continued standardization, is now being applied to large database operations in many countries. However, despite the high discrimination ability and superior analysis ability, there is a possibility of misjudgment when checking family relations due to the relatively high mutation rate (-10 ^-3 ), especially when searching for large databases such as MIA business. Problems have been identified that do not show enough discrimination if family numbers are limited.

Therefore, the need for complementary testing system using more stable single nucleotide polymorphism (SNP) markers (mutation rate ~ 10 ^-6 ) has been raised to enhance the discrimination of personal identification using STR markers and to correct the result of mutation. Research development is underway.

Krawczak (Electrophoresis. 1999 Jun; 20 (8): 1676-81) reported that the paternity exclusion power of one STR marker was similar to 4.2 SNPs with an allele expression frequency of 0.5. Legal Med. 114 (2001) 204-210) reported that the negative exclusion power of 50 SNPs with an allelic expression frequency of 0.2 to 0.8 was 0.999, indicating a predictive power similar to 12 STRs.

In addition, Ayres et al. (Forensic Sci. Int. 154 (2005) 167-172) require 50-60 SNP markers with allele expression frequencies of 0.3 to 0.7 to determine the paternity index of a three-member family. For single-family households, 70-80 SNP markers were required.

Recently, the development of universal SNP panel kits with high heterozygosity (> 0.45) and similar frequency of expression in many races (Fst <0.01) has been actively studied due to the high commercial potential. A panel of 40 SNPs with similar expression frequency in 40 populations was developed using TaqMan probe DB (90483 SNPs) for four races of ABI. However, the SNP panel includes a large number of SNPs distributed in the genetic locus and has a limitation that extensive SNP search cannot be performed.

Vallone et al. (Forensic Sci Int. 2005 May 10; 149 (2-3): 279-86) confirmed the final 12 identifications by analyzing 70 SNP markers in Caucasian, African-American and Latin Americans. Although SNP markers have been selected for use, there is a suggestion that screening more than 35 SNP markers can enhance discrimination in personal identification using SNPs.

As a result of the SNPforID project in five European countries, including Germany, racial frequency information for 52 individual SNP markers has been published in the SNPforID DB. However, markers were selected mainly for Westerners, and about 30% of SNPs contained SNPs with relatively low discrimination with a minor allele frequency (MAF) of less than 20% in the Hapmap Asian race. Therefore, there is a limitation in applying the SNP marker to Koreans.

Selecting valid SNP markers requires extensive screening through large-scale SNP data, and in particular, developing SNP markers that can distinguish Koreans with high discrimination power. Therefore, the present inventors analyzed the entire genome of Koreans using Affymetrix 500K to identify the frequency of expression of 500,000 SNPs and developed a personal identification SNP marker suitable for application in identification in 4 HapMap races and Korean populations. .

It is an object of the present invention to provide polynucleotides or their complementary polynucleotides comprising a single nucleotide polymorphism (SNP) associated with personal identification.

It is still another object of the present invention to provide amplification primers or personal identification probes for personal identification consisting of polynucleotides or their complementary polynucleotides comprising a single base polymorphism related to personal identification.

Still another object of the present invention is to provide a personal identification composition or microarray comprising the probe.

It is still another object of the present invention to provide a method of identifying an individual using a polynucleotide comprising a single base polymorphism associated with personal identification or a complementary polynucleotide thereof.

In order to achieve the above object, the present invention provides a polynucleotide selected from the group consisting of nucleotide sequences of SEQ ID NOs: 1 to 92, or a complementary polynucleotide thereof, wherein the SNP (monobasic polymorphism) The present invention provides a personally identifiable polynucleotide marker consisting of a polynucleotide consisting of 10 or more contiguous nucleotides, including the 101 st base.

In one embodiment of the invention, the polynucleotide marker is a polynucleotide of SEQ ID NO: 1 to 45 and 48 to 90 or a polynucleotide consisting of 10 or more contiguous nucleotides including the SNP located in the 101th position in the complementary polynucleotide thereof May be a combination.

The present invention also provides a polynucleotide selected from the group consisting of nucleotide sequences of SEQ ID NOs: 1 to 92, or a complementary polynucleotide thereof, wherein the SNP is present at the 101 st position of the polynucleotide, and includes 10 or more bases including the 101 st base. It provides a personal identification composition comprising a personal identification polynucleotide marker consisting of a polynucleotide consisting of consecutive nucleotides.

In one embodiment of the present invention, the personally identifiable composition comprises at least 10 SNPs comprising the polynucleotides of SEQ ID NOs: 1 to 45 and 48 to 90 or the complementary polynucleotides thereof at the 101st position as the personally identifiable polynucleotide markers. Combinations of polynucleotides consisting of contiguous nucleotides.

The present invention also provides a polynucleotide selected from the group consisting of nucleotide sequences of SEQ ID NOs: 1 to 92, or a complementary polynucleotide thereof, wherein the SNP is present at the 101 st position of the polynucleotide, and includes 10 or more bases including the 101 st base. Provided are compositions for identifying paternity of three or more families consisting of parents, parents and children, including a personally identifiable polynucleotide marker consisting of polynucleotides consisting of contiguous nucleotides.

In one embodiment of the present invention, the composition for identifying paternity comprises a polynucleotide marker of SEQ ID NOs: 1 to 45 and 48 to 90 or the SNP located at the 101st position in the complementary polynucleotide thereof as a personal identification polynucleotide marker. Combinations of polynucleotides consisting of ten or more contiguous nucleotides.

The present invention also provides a polynucleotide selected from the group consisting of SEQ ID NOs: 46, 47, 91 and 92, or a complementary polynucleotide thereof, wherein the SNP is present at the 101 st position of the polynucleotide and includes at least 10 bases including the 101 st base. Provided is a gender-identified polynucleotide marker composed of polynucleotides consisting of contiguous nucleotides.

In one embodiment of the invention, the polynucleotide marker is a polynucleotide of SEQ ID NO: 46, 47, 91 and 92 or a polynucleotide consisting of 10 or more contiguous nucleotides including the SNP located in the 101 position in the complementary polynucleotide thereof May be a combination.

The present invention also provides a polynucleotide selected from the group consisting of SEQ ID NOs: 46, 47, 91, and 92, or a complementary polynucleotide thereof, wherein the SNP is present at the 101 st position of the polynucleotide, and includes the 10 th base including the 101 st base. It provides a sex confirming composition comprising a sex confirming polynucleotide marker composed of a polynucleotide consisting of the above consecutive nucleotides.

In one embodiment of the present invention, the sex identification composition is a sex identification polynucleotide marker containing at least 10 polynucleotides of SEQ ID NOs: 46, 47, 91 and 92 or the SNP located in the 101th complementary polynucleotide thereof Combinations of polynucleotides consisting of contiguous nucleotides.

The usefulness of monobasic polymorphic markers used to identify individuals can be determined by "personal identification index" or "matching probability (PI)" and "power of exclusion".

As used herein, "personal identification" refers to the identification of individual samples not derived from the same person through DNA profiling, broadly including paternity and gender identification.

In one embodiment of the invention, the individual may be Korean or HapMap four races. The four HapMap races (see www.hapmap.org) are Utah residents from the Yoruba (YRI) in Ibadan, Japan (JPT) in Tokyo, the Han Chinese (CHB) in Beijing, China, and the ancestors of Northern and Western Europe. (CEU).

"Personal Identification Index" is one of the criteria for judging the usefulness as a marker for personal identification, and refers to the ratio of the probability that the DNA of the sample is derived from the same person and that the DNA profile is coincident, but that the DNA profile is coincidentally. . Personal identification is the inverse of the pair probability in DNA analysis from a single source.

"Matching probability (PI)" refers to the probability that a particular genotype will be found by chance in any population. For a combination of several markers, its usefulness as a marker for personal identification is determined by cumulative pair probability.

As used herein, "Power of Exclusion" (PE) means the ability to exclude relatives of men, especially fathers, from the possibility of being a biological father. Parental exclusion depends on the genetics of both mother and child and the ethnic background of the mother and father.

For accurate personal identification, markers with high personal identification indexes, ie, low probability of pairing or cumulative pairing, and high derogatory power are required.

Polynucleotide, which is a monobasic polymorphic marker for personal identification according to an embodiment of the present invention, has a pair probability of 0.345 to 0.465, and has a power of exclusion (PE), as shown in Table 1 below. . In addition, the cumulative pair probability of the personal identification polynucleotide marker consisting of 10 or more contiguous nucleotides including the SNPs located at the 101st positions of SEQ ID NOs: 1 to 45 and 48 to 90 is 3.61E-37. Lower than the kit (4.9E-20) consisting of 17 short tandem repeat (STR) markers being used.

In the present invention, "polynucleotide" may be DNA or RNA. The polynucleotide may also be in single- or double-stranded form. The polynucleotide may also be composed of natural nucleotides, as well as natural nucleotides, analogs of natural nucleotides, sugars, bases or phosphoric acid sites of natural nucleotides, provided that they have the property of hybridizing to complementary nucleotides by hydrogen bonding. Nucleotides selected from the group consisting of nucleotides and combinations thereof (Scheit, Nucleotide Analogs, John Wiley, New York (1980); Uhlman and Peyman, Chemical Reviews , 90: 543-584 (1990)). . In addition, the polynucleotide may comprise a peptide nucleic acid (PNA). The polynucleotide may, for example, have a detectable label (e.g., Cy3, Cy5 fluorescent material), e.g., at the 3 'end, for ease of detection of the polynucleotide or complex to which it is bound in an assay. Or it may be attached to the 5 'end.

In one embodiment of the invention, the polynucleotides exhibit monobasic polymorphism at the SNP site. When one single-stranded polynucleotide is associated with a personal identification, it may be determined that the polynucleotide complementary to the single-stranded polynucleotide is also naturally associated with the personal identification. Thus, a polynucleotide according to one embodiment of the present invention includes a single stranded polynucleotide associated with a personal identification having one specific sequence and a polynucleotide having a sequence complementary thereto. For example, the polynucleotide of SEQ ID NO: 1 has the "C or T" nucleotide 101 (SNP position). In this case, the polynucleotide comprises the "C or T" nucleotide of the 101st (SNP position) and corresponds to the 101st (SNP position) as well as 10 or more consecutive nucleotides selected from the polynucleotide of SEQ ID NO: 1. Complementary single-stranded polynucleotides having “G or A” nucleotides in position. In this aspect, all sequences herein are indicated based on the sequences in the sense strand in genomic DNA, unless otherwise noted.

In the present invention, single nucleotide polymorphism (SNP) is used in the meaning commonly known in the art. SNPs may represent single nucleotide polymorphisms present in the genome within a population. The SNP may be one or more frequency of the minor allele of the SNP in the population.

In one embodiment of the invention, the polynucleotide may be from 10 to 100 nucleotides in length. For example, the polynucleotide may be 10 to 50 nucleotides in length, or 10 to 30 nucleotides in length.

In one embodiment of the invention, the polynucleotide may be a primer or a probe. "Primer" refers to a single-stranded polynucleotide that can act as a starting point in the polymerization of nucleotides by polymerases. For example, the primers can serve as a starting point for template-directed DNA synthesis in suitable conditions and in suitable buffers, i.e., in the presence of four different nucleoside triphosphates and polymerases. Single-stranded polynucleotides. Suitable length of the primer can vary depending on various factors, such as temperature and the use of the primer. The primer may be 15 to 30 nucleotides in length. In general, the shorter the length of the primer, the more capable of forming a hybrid composite that is sufficiently stable with the template at low annealing temperatures.

The sequence of the primer does not need to have a sequence that is completely complementary to some sequences of the template, and it is sufficient to have complementarity within a range capable of hybridizing with the template to perform primer-specific functions. Thus, primers include not only the polynucleotide itself but also those which can act as starting points in a polymerization reaction as sequences that specifically hybridize to the polynucleotide. For example, not only sequences perfectly complementary to the polynucleotide of SEQ ID NO: 1, but also sequences having complementarity within a range capable of hybridizing to the sequence and acting as a primer. The design of the primer can be easily carried out by those skilled in the art with reference to the given sequence of the target nucleic acid to be amplified. For example, it can be designed using a commercially available primer design program. Commercially available primer design programs include, but are not limited to, for example, the PRIMER 3 program.

In one embodiment of the invention, when a polynucleotide is used as a PCR primer, in addition to the polynucleotide, it may include a primer that specifically binds to its complementary strand.

"Probe" refers to a polynucleotide that specifically binds to a specific target sequence. The polynucleotide may be DNA or RNA. The polynucleotide may be in the form of a single strand. The polynucleotide may also be composed of natural nucleotides, as well as natural nucleotides, analogues of natural nucleotides, sugars, bases or phosphoric acid sites of natural nucleotides, provided that they have the property of hybridizing to complementary nucleotides by hydrogen bonding. Nucleotides selected from the group consisting of nucleotides and combinations thereof. The polynucleotide includes PNA. In addition, the polynucleotide may have a detectable label (e.g., a Cy3, Cy5 fluorescent substance), e.g., at the 3 'end, for example, for ease of detection of the polynucleotide or complex to which it is bound in an assay. It may be attached to the 5 'end.

The probe may be a sequence that is completely complementary to the polynucleotide comprising a SNP site. In addition, the probe may have a sequence that is substantially complementary within a range that does not prevent specific hybridization to the polynucleotide including the SNP site. In addition, the probe may be one having a modified nucleotide within a range that does not impair specific hybridization to the polynucleotide including the SNP site. Examples of the probes include a perfect match probe consisting of a sequence completely complementary to the polynucleotide comprising a SNP site and a complete match for all sequences except the SNP site, for the polynucleotide comprising a SNP site. It may be selected from the group consisting of mismatch probes having complementary sequences.

The primers and probes can be used to amplify or confirm the presence of a nucleotide sequence having an allele specific for the SNP site.

Another embodiment of the present invention, in the polynucleotide selected from the group consisting of the nucleotide sequence of SEQ ID NO: 1 to 92 or a complementary polynucleotide thereof, SNP is present at position 101 of the polynucleotide, and the 101 base It provides a microarray for personal identification comprising a personal identification polynucleotide marker consisting of a polynucleotide consisting of 10 or more consecutive nucleotides.

In one embodiment of the present invention, the personally identifiable polynucleotide marker consists of 10 or more contiguous nucleotides, including the SNP located 101st in the polynucleotides of SEQ ID NOs: 1 to 45 and 48 to 90 or complementary polynucleotides thereof. It can be a combination of polynucleotides.

The polynucleotide has the characteristics as described above.

In the present invention, "microarray" means that the polynucleotide is immobilized to a high density in the separated region of the substrate surface. The microarray may be one in which the region is arranged on a substrate at a density of, for example, 400 / cm ² or more, 10 ³ / cm ² , or 10 ⁴ / cm ² .

Another embodiment of the present invention, in the polynucleotide selected from the group consisting of the nucleotide sequence of SEQ ID NO: 1 to 92 or a complementary polynucleotide thereof, SNP is present at position 101 of the polynucleotide, and the 101 base It provides a kit for personal identification comprising a personal identification polynucleotide marker consisting of a polynucleotide consisting of 10 or more consecutive nucleotides.

The polynucleotide marker has the characteristics as described above. The polynucleotide markers in the kit may be in the form of microarrays that are densely arranged in discrete regions on the substrate surface.

In one embodiment of the invention, the polynucleotide marker is a polynucleotide of SEQ ID NO: 1 to 45 and 48 to 90 or a polynucleotide consisting of 10 or more contiguous nucleotides including the SNP located in the 101 position in the complementary polynucleotide thereof May be a combination.

The kit may be a kit for specifically amplifying the polynucleotide and identifying an individual or confirming paternity through the presence or absence of an amplification product. In this case, the kit may include the polynucleotide as a primer, and at the same time, include a reagent required for amplification. The amplification reagent may include, for example, dNTPs, polymerases, and appropriate buffers. The primers are, for example, wherein the nucleotide sequence corresponding to the SNP site forms the 3 'terminal nucleotide of the primer, and the 3' terminal nucleotide is complementary (specific primer) or not complementary to the nucleotide of the SNP site. (Nonspecific primer). The nonspecific primer may include sequences that are not complementary to the 3 ′ terminal nucleotides as well as other sites. The kit may also include instructions for use. The instructions may be used, for example, when a target sequence is amplified in an amplification reaction using the specific primer, and a target sequence is not amplified in the amplification reaction using the non-specific primer, a target associated with personal identification among samples used for amplification. It may include a description of the outcome determination, including a description of determining that the sequence exists and identifying the individual from the results.

The kit may be a kit for hybridizing the polynucleotide or a probe derived therefrom with a nucleic acid in a sample, and identifying an individual from the hybridization result. In this case, the kit may include the probe and a reagent required for hybridization. Reagents required for hybridization may include, for example, hybridization buffers. The nucleic acid may be amplified or not amplified. Thus, the kit may further comprise a reagent for amplifying the nucleic acid. The nucleic acid may be labeled with a detectable label. The kit may comprise a perfect match probe to the polynucleotide or a mismatch probe complementary at all sites except the SNP site in the polynucleotide. The probe may be in the form of a microarray fixed to a plurality of divided regions on the substrate. The kit may also include instructions for use. The instructions are for example a profile obtained by each probe for each sample if the target sequence is detected in a hybridization reaction using the fully complementary probe and the target sequence is not detected in the hybridization reaction using the mismatch probe. May include a description of the result determination, including a description of the comparison and the identification of the individual from the results, or the determination of paternity.

In another embodiment of the present invention, the kit may be a kit for confirming paternity of three or more families consisting of parents and children.

Another embodiment of the present invention is a polynucleotide selected from the group consisting of SEQ ID NOs: 46, 47, 91, and 92 or a complementary polynucleotide thereof, wherein the SNP is present at position 101 of the polynucleotide, and Provided is a kit for identifying a gender of an individual, comprising a gender-identifying polynucleotide marker consisting of a polynucleotide consisting of 10 or more consecutive nucleotides.

In one embodiment of the present invention, the gender-identifying polynucleotide marker is composed of 10 or more contiguous nucleotides including the SNP located at the 101st position in the polynucleotides of SEQ ID NOs: 46, 47, 91 and 92 or their complementary polynucleotides It can be a combination of polynucleotides.

The polynucleotide has the characteristics as described above. In the kit, the polynucleotides may be in the form of microarrays that are densely arranged in discrete regions on the substrate surface.

Another embodiment of the present invention,

Providing an isolated nucleic acid sample; And

In a polynucleotide selected from the group consisting of the nucleotide sequences of SEQ ID NOs: 1 to 92, or a complementary polynucleotide thereof, SNP is present at position 101 of the polynucleotide, and is composed of 10 or more consecutive nucleotides including the 101st base. Provided is a method of identifying an individual comprising determining the nucleotide of the SNP position of a personally identifiable polynucleotide marker composed of polynucleotides.

In one embodiment of the present invention, the personally identifiable polynucleotide marker consists of 10 or more contiguous nucleotides, including the SNP located 101st in the polynucleotides of SEQ ID NOs: 1 to 45 and 48 to 90 or complementary polynucleotides thereof. It can be a combination of polynucleotides.

According to one embodiment of the present invention, a method for identifying an individual includes providing an isolated nucleic acid sample. Methods of separating nucleic acids from an individual are known in the art. For example, it can be isolated by directly isolating DNA from tissue or cells or by amplifying a specific region by nucleic acid amplification methods such as PCR. The isolated nucleic acid sample includes not only purely isolated nucleic acid but also cell lysates containing crude separated nucleic acid, for example, nucleic acid. Such nucleic acid amplification methods include PCR, ligase chain reaction (LCR), transcription amplification, self-sustained sequence replication, and nucleic acid based sequence amplification (NASBA). The isolated nucleic acid may be DNA or RNA. The DNA may be genomic DNA, cDNA or recombinant DNA. The RNA may be mRNA.

The method also includes determining a nucleotide at the SNP position of the personally identifiable polynucleotide marker.

Methods of determining nucleotides of SNP positions are well known in the art. For example, the nucleotide at the SNP position can be determined directly by known nucleotide sequencing methods. Nucleotide sequencing methods may include a staggered (or dideoxy) method or a Maksam-Gilbert (chemical cleavage) method. In addition, the nucleotide of the SNP position can be determined by hybridizing a probe including the sequence of the SNP position with the polynucleotide of interest and analyzing the hybridization result. The degree of hybridization can be confirmed, for example, by labeling the detectable label to the target nucleic acid, detecting the hybridized target nucleic acid, or by an electrical method or the like. In addition, a single base primer extension (SBE) method may be used.

In the method, the step of determining the nucleotide of the SNP site may include hybridizing the nucleic acid sample to a microarray to which the polynucleotide marker is immobilized; And detecting the hybridization result.

The method of identifying an individual according to an embodiment of the present invention may further comprise comparing the nucleotide of the SNP position of the polynucleotide marker for the determined personal identification with the result of the control sample. The control sample may be the DNA of the individual to be compared or the registered DNA profile of the individual.

The method of identifying an individual according to an embodiment of the present invention may further include identifying a nucleotide at the SNP position of the polynucleotide marker for the determined personal identification and calculating a personal identification index.

In the polynucleotide markers used in the method of identifying the individual, it is determined that the genotypes between the samples to be compared are unequal, i.e., when the nucleotides of the SNP positions are different, they are not the same person, in which case the personal identification index is zero. On the other hand, when all of the nucleotides at the SNP positions of the polynucleotide markers used match, it is determined as genotype matching, and the personal identification index is calculated. The personal identification index can be calculated as follows:

Homozygousity =

Heterojunction =

Power of discrimination (PD) =

Discrimination Index (DI) = 1-PD

In the above, pi means the allele frequency.

The identification power increases as the number of markers increases, and the probability of appearing a person having the same genotype as a specific person can be expressed as 1 / DI.

Another embodiment of the invention is a method of identifying the sex of an individual,

Providing an isolated nucleic acid sample; And

In a polynucleotide selected from the group consisting of SEQ ID NOs: 46, 47, 91, and 92, or a complementary polynucleotide thereof, SNP is present at position 101 of the polynucleotide and is selected from the group consisting of 10 or more consecutive nucleotides including the 101st base. It provides a method comprising the step of determining the nucleotide of the SNP position of the sex identification polynucleotide marker consisting of a polynucleotide consisting of.

According to one embodiment of the present invention, a method for identifying a gender of an individual includes providing an isolated nucleic acid sample. Methods of separating nucleic acids from an individual are known in the art. For example, it can be isolated by directly isolating DNA from tissue or cells or by amplifying a specific region by nucleic acid amplification methods such as PCR. The isolated nucleic acid sample includes not only purely isolated nucleic acid but also cell lysates containing crude separated nucleic acid, for example, nucleic acid. Such nucleic acid amplification methods include PCR, ligase chain reaction (LCR), transcriptional amplification, self-retaining sequence replication, and nucleic acid based sequence amplification (NASBA). The isolated nucleic acid may be DNA or RNA. The DNA may be genomic DNA, cDNA or recombinant DNA. The RNA may be mRNA.

The method also includes determining a nucleotide at the SNP position of the gender identifying polynucleotide.

Methods of determining nucleotides of SNP positions are well known in the art. For example, nucleotide sequencing of known nucleic acids can directly determine the nucleotides of the SNP positions. Nucleotide sequencing methods may include a staggered (or dideoxy) method or a Maksam-Gilbert (chemical cleavage) method. In addition, the nucleotide of the SNP position can be determined by hybridizing a probe including the sequence of the SNP position with the polynucleotide of interest and analyzing the hybridization result. The degree of hybridization can be confirmed, for example, by labeling the detectable label to the target nucleic acid, detecting the hybridized target nucleic acid, or by an electrical method or the like. In addition, a single base extension method may be used.

In the method, determining the polynucleotide of the SNP site may include hybridizing the nucleic acid sample to a microarray in which the polynucleotide is immobilized; And detecting the hybridization result.

According to one embodiment of the present invention, a method for identifying a gender of an individual may further include comparing a nucleotide at the SNP position of the determined polynucleotide for identification with a control sample. The control sample may be genotype of the SNP position of the sex-identifying polynucleotide used above obtained from male and female. The male control sample has a genotype of AG and GG for the markers of SEQ ID NOs: 46 and 47, respectively, and a genotype of AA for the markers of SEQ ID NOs: 91 and 92, respectively. On the other hand, the female control sample has a genotype of GG for the marker of SEQ ID NO: 46, but no results are detected for the markers of SEQ ID NOs: 47, 91 and 92. Therefore, gender can be confirmed by comparing the analysis result of the sample for the marker for confirming sex with the male and female controls.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, an Example is for illustrating this invention and this invention is not limited by these.

Polynucleotides comprising a monobasic polymorphism or a complementary polynucleotide marker thereof and a composition comprising the same according to the present invention can be usefully used to identify an individual. In addition, primers or probes composed of polynucleotides or complementary polynucleotides thereof, and kits comprising the same may be usefully used to identify individuals.

Example 1 Selection of SNP Marker for Personal Identification

(1) Target classification

Genotyping of Affymetrix 500K SNPs was performed on 70 Korean specimens to set the minimum level of call rate (sample rate at which total genotypes were obtained) to 95%, with an average of 99.5% The data of rate was obtained.

70 people were analyzed based on p-value cut-off (p> 0.05) by constructing DB of frequency of each SNP and performing Hardy-Weinberg Equilibrium (HWE) test. The GRR analysis reconfirmed that the sample was independent of the chromosome (Graphical Representation of Relationships http://www.sph.umich.edu/csg/abecasis/GRR).

(2) SNP marker selection

Korean and HapMap four races (see www.hapmap.org), namely the Yoruba (YRI) in Ibadan, Nigeria (JPT) in Tokyo, the Han Chinese (CHB) in Beijing, China, and the ancestors of Northern and Western Europe In the result of analysis of Affimetrix 500K SNP chip of Utah resident (CEU), we selected SNP markers with 40-50% of minor allele frequency (MAF) and genotype distribution of 1: 2: 1. Markers were selected that were not associated with a specific gene or disease (selection of non-genetic SNPs not included in the gene region ± 2 kb) (except SNPs on X and Y chromosomes).

13,663 SNPs with PCR annealing temperature of 55 ° C. and SNP kidney mix (CA / GT, CT / AG) were selected (AT / CG mutation and triallele resulting in allele confusion depending on the strand) Except for the case where two or more products were amplified by In silico PCR among the highly homologous SNPs and designed SNPs through the sequence blast. Afterwards, SNPs on the same chromosome were analyzed by linkage disequilibrium (LD) to select SNPs without LD relations, except when the distance between the two SNPs was greater than 500 kb, except when r ² > 0.3. Identifying flanking sequences up to 250 nucleotides in both directions and designing assay panels using information provided by Beckman coulter (www.autoprimer.com) for the design of multiplex PCR primers 135 markers and 4 gender identification SNP markers were selected as candidates. 92 markers for which SNP-IT analysis was completed using the dual SNPstream® UHT system are shown in Table 1.

Table 1. Selected SNP Markers for Personal Identification

No rs_number chromosome allele_A allele_B Freq_A Freq_B AA AB BB One rs515603 chr1 A G 0.478 0.522 19 47 23 2 rs10785736 chr1 A G 0.593 0.407 30 42 14 3 rs10920379 chr1 A G 0.494 0.506 21 46 22 4 rs7527185 chr1 A G 0.522 0.478 24 45 20 5 rs902329 chr1 A G 0.530 0.470 24 41 19 6 rs10926595 chr1 A G 0.562 0.438 28 44 17 7 rs10884796 chr10 A G 0.416 0.584 14 46 29 8 rs12286769 chr11 A G 0.506 0.494 25 40 24 9 rs10791403 chr11 A G 0.506 0.494 22 45 21 10 rs1487602 chr12 A G 0.427 0.573 14 48 27 11 rs12876644 chr13 A G 0.483 0.517 23 40 26 12 rs12585235 chr13 A G 0.489 0.511 17 53 19 13 rs9540071 chr13 A G 0.382 0.618 16 36 37 14 rs9523953 chr13 A G 0.567 0.433 27 47 15 15 rs2402341 chr14 A G 0.438 0.562 20 38 31 16 rs4906699 chr15 A G 0.545 0.455 23 50 15 17 rs691 chr15 A G 0.528 0.472 27 40 22 18 rs424165 chr16 A G 0.612 0.388 35 39 15 19 rs1812521 chr16 A G 0.433 0.567 16 45 28 20 rs4407150 chr18 A G 0.483 0.517 17 50 20 21 rs12965342 chr18 A G 0.511 0.489 19 53 17 22 rs1943338 chr18 A G 0.584 0.416 31 42 16 23 rs1563172 chr2 A G 0.559 0.441 29 37 19 24 rs1427446 chr2 A G 0.449 0.551 21 38 30 25 rs2241225 chr2 A G 0.403 0.597 12 47 29 26 rs6744937 chr2 A G 0.580 0.420 26 49 12 27 rs6442180 chr3 A G 0.466 0.534 18 47 24 28 rs6549566 chr3 A G 0.539 0.461 25 46 18 29 rs1709915 chr3 A G 0.461 0.539 17 48 24 30 rs6447614 chr4 A G 0.539 0.461 29 38 22 31 rs970022 chr4 A G 0.556 0.444 32 35 22 32 rs2613019 chr4 A G 0.444 0.556 14 51 24 33 rs4501321 chr5 A G 0.545 0.455 25 46 17 34 rs10075131 chr5 A G 0.433 0.567 19 39 31 35 rs11952230 chr5 A G 0.449 0.551 16 48 25 36 rs7707575 chr5 A G 0.415 0.585 15 43 30 37 rs1883181 chr6 A G 0.483 0.517 15 56 18 38 rs6900780 chr6 A G 0.517 0.483 25 42 22 39 rs2736966 chr7 A G 0.568 0.432 25 50 13 40 rs6947796 chr7 A G 0.478 0.522 23 39 27 41 rs624667 chr7 A G 0.500 0.500 22 45 22 42 rs12546986 chr8 A G 0.545 0.455 27 43 19 43 rs1875067 chr8 A G 0.590 0.410 35 35 19 44 rs4876357 chr8 A G 0.478 0.522 23 39 27 45 rs10811474 chr9 A G 0.404 0.596 15 42 32 46 rs2444398 chrX, Y A G One 40 48 47 rs2521574 chrY A G 0 One 0 0 41 48 rs7921967 chr10 A G 0.472 0.528 17 50 22 49 rs11599718 chr10 A G 0.489 0.511 24 40 26 50 rs7298551 chr12 A G 0.483 0.517 21 45 24 51 rs1166235 chr12 A G 0.517 0.483 26 40 23 52 rs12820285 chr12 A G 0.550 0.450 26 47 17 53 rs11060771 chr12 A G 0.517 0.483 25 42 22 54 rs2039623 chr13 A G 0.483 0.517 18 50 21  55 rs9523089 chr13 A G 0.539 0.461 27 43 20 56 rs408283 chr14 A G 0.449 0.551 20 40 29 57 rs7160372 chr14 A G 0.444 0.556 19 42 29 58 rs11634785 chr15 A G 0.389 0.611 15 40 35 59 rs31042 chr16 A G 0.461 0.539 19 44 26 60 rs11076262 chr16 A G 0.556 0.444 24 52 14 61 rs7192715 chr16 A G 0.511 0.489 22 48 20 62 rs8058011 chr16 A G 0.417 0.583 14 47 29 63 rs2470209 chr17 A G 0.411 0.589 13 48 29 64 rs12607426 chr18 A G 0.517 0.483 26 41 23 65 rs6507244 chr18 A G 0.635 0.365 35 43 11 66 rs4926111 chr19 A G 0.640 0.360 32 50 7 67 rs8102497 chr19 A G 0.444 0.556 19 42 29 68 rs4027132 chr2 A G 0.500 0.500 22 46 22 69 rs330625 chr2 A G 0.456 0.544 20 42 28 70 rs6711114 chr2 A G 0.428 0.572 18 41 31 71 rs4349283 chr2 A G 0.428 0.572 20 37 33 72 rs12613725 chr2 A G 0.511 0.489 20 51 18 73 rs3761161 chr20 A G 0.399 0.601 17 37 35 74 rs6014763 chr20 A G 0.411 0.589 15 44 31 75 rs6006426 chr22 A G 0.572 0.428 32 39 19 76 rs7288176 chr22 A G 0.439 0.561 15 49 26 77 rs12695977 chr3 A G 0.477 0.523 17 50 21 78 rs2127657 chr4 A G 0.589 0.411 31 44 15 79 rs4141387 chr4 A G 0.410 0.590 15 43 31 80 rs6850503 chr4 A G 0.389 0.611 12 46 32 81 rs2642781 chr5 A G 0.539 0.461 22 53 15 82 rs6596805 chr6 A G 0.466 0.534 20 43 26 83 rs1321167 chr6 A G 0.500 0.500 19 52 19 84 rs9371835 chr6 A G 0.494 0.506 20 49 21 85 rs11971741 chr7 A G 0.517 0.483 22 49 19 86 rs4722616 chr7 A G 0.600 0.400 33 42 15 87 rs7792547 chr7 A G 0.506 0.494 22 47 21 88 rs2468177 chr8 A G 0.456 0.544 19 44 27 89 rs7038176 chr9 A G 0.461 0.539 19 45 26 90 rs7027501 chr9 A G 0.494 0.506 20 49 21 91 rs2032654 chrY A G One 0 41 0 0 92 rs9341273 chrY A G One 0 41 0 0

Table 1. (continued)

No fail total Call
rate AA_freq AB_freq BB_freq panel PI PE One One 89 98.89 0.213 0.528 0.258 AG1 0.391 0.213 2 4 86 95.56 0.349 0.488 0.163 AG1 0.387 0.178 3 One 89 98.89 0.236 0.517 0.247 AG1 0.384 0.203 4 One 89 98.89 0.270 0.506 0.225 AG1 0.379 0.192 5 6 84 93.33 0.286 0.488 0.226 AG1 0.371 0.177 6 One 89 98.89 0.315 0.494 0.191 AG1 0.380 0.183 7 One 89 98.89 0.157 0.517 0.326 AG1 0.398 0.203 8 One 89 98.89 0.281 0.449 0.270 AG1 0.354 0.147 9 2 88 97.78 0.250 0.511 0.239 AG1 0.381 0.198 10 One 89 98.89 0.157 0.539 0.303 AG1 0.408 0.224 11 One 89 98.89 0.258 0.449 0.292 AG1 0.354 0.147 12 One 89 98.89 0.191 0.596 0.213 AG1 0.437 0.286 13 One 89 98.89 0.180 0.404 0.416 AG1 0.369 0.117 14 One 89 98.89 0.303 0.528 0.169 AG1 0.399 0.213 15 One 89 98.89 0.225 0.427 0.348 AG1 0.354 0.131 16 2 88 97.78 0.261 0.568 0.170 AG1 0.420 0.254 17 One 89 98.89 0.303 0.449 0.247 AG1 0.355 0.147 18 One 89 98.89 0.393 0.438 0.169 AG1 0.375 0.139 19 One 89 98.89 0.180 0.506 0.315 AG1 0.387 0.192 20 3 87 96.67 0.195 0.575 0.230 AG1 0.421 0.262 21 One 89 98.89 0.213 0.596 0.191 AG1 0.437 0.286 22 One 89 98.89 0.348 0.472 0.180 AG1 0.376 0.164 23 5 85 94.44 0.341 0.435 0.224 AG1 0.356 0.137 24 One 89 98.89 0.236 0.427 0.337 AG1 0.352 0.131 25 2 88 97.78 0.136 0.534 0.330 AG1 0.412 0.219 26 3 87 96.67 0.299 0.563 0.138 AG1 0.426 0.249 27 One 89 98.89 0.202 0.528 0.270 AG1 0.393 0.213 28 One 89 98.89 0.281 0.517 0.202 AG1 0.387 0.203 29 One 89 98.89 0.191 0.539 0.270 AG1 0.400 0.224 30 One 89 98.89 0.326 0.427 0.247 AG1 0.350 0.131 31 One 89 98.89 0.360 0.393 0.247 AG1 0.345 0.110 32 One 89 98.89 0.157 0.573 0.270 AG1 0.426 0.260 33 2 88 97.78 0.284 0.523 0.193 AG1 0.391 0.208 34 One 89 98.89 0.213 0.438 0.348 AG1 0.359 0.139 35 One 89 98.89 0.180 0.539 0.281 AG1 0.402 0.224 36 2 88 97.78 0.170 0.489 0.341 AG1 0.384 0.178 37 One 89 98.89 0.169 0.629 0.202 AG1 0.465 0.327 38 One 89 98.89 0.281 0.472 0.247 AG1 0.363 0.164 39 2 88 97.78 0.284 0.568 0.148 AG1 0.425 0.254 40 One 89 98.89 0.258 0.438 0.303 AG1 0.351 0.139 41 One 89 98.89 0.247 0.506 0.247 AG1 0.378 0.192 42 One 89 98.89 0.303 0.483 0.213 AG1 0.371 0.173 43 One 89 98.89 0.393 0.393 0.213 AG1 0.355 0.110 44 One 89 98.89 0.258 0.438 0.303 AG1 0.351 0.139 45 One 89 98.89 0.169 0.472 0.360 AG1 0.380 0.164 46 One 89 98.89 0.01 0.45 0.54 AG1 47 49 41 45.5 0 0 1.00 AG1 48 One 89 98.89 0.191 0.562 0.247 AG2 0.413 0.248 49 0 90 100 0.267 0.444 0.289 AG2 0.352 0.143 50 0 90 100 0.233 0.500 0.267 AG2 0.376 0.188 51 One 89 98.89 0.292 0.449 0.258 AG2 0.354 0.147 52 0 90 100 0.289 0.522 0.189 AG2 0.392 0.208 53 One 89 98.89 0.281 0.472 0.247 AG2 0.363 0.164 54 One 89 98.89 0.202 0.562 0.236 AG2 0.412 0.248  55 0 90 100 0.300 0.478 0.222 AG2 0.368 0.169 56 One 89 98.89 0.225 0.449 0.326 AG2 0.359 0.147 57 0 90 100 0.211 0.467 0.322 AG2 0.366 0.160 58 0 90 100 0.167 0.444 0.389 AG2 0.377 0.143 59 One 89 98.89 0.213 0.494 0.292 AG2 0.375 0.183 60 0 90 100 0.267 0.578 0.156 AG2 0.429 0.265 61 0 90 100 0.244 0.533 0.222 AG2 0.394 0.218 62 0 90 100 0.156 0.522 0.322 AG2 0.401 0.208 63 0 90 100 0.144 0.533 0.322 AG2 0.409 0.218 64 0 90 100 0.289 0.456 0.256 AG2 0.356 0.151 65 One 89 98.89 0.393 0.483 0.124 AG2 0.403 0.173 66 One 89 98.89 0.360 0.562 0.079 AG2 0.451 0.248 67 0 90 100 0.211 0.467 0.322 AG2 0.366 0.160 68 0 90 100 0.244 0.511 0.244 AG2 0.381 0.197 69 0 90 100 0.222 0.467 0.311 AG2 0.364 0.160 70 0 90 100 0.200 0.456 0.344 AG2 0.366 0.151 71 0 90 100 0.222 0.411 0.367 AG2 0.353 0.121 72 One 89 98.89 0.225 0.573 0.202 AG2 0.420 0.260 73 One 89 98.89 0.191 0.416 0.393 AG2 0.364 0.124 74 0 90 100 0.167 0.489 0.344 AG2 0.385 0.178 75 0 90 100 0.356 0.433 0.211 AG2 0.359 0.136 76 0 90 100 0.167 0.544 0.289 AG2 0.408 0.229 77 2 88 97.78 0.193 0.568 0.239 AG2 0.417 0.254 78 0 90 100 0.344 0.489 0.167 AG2 0.385 0.178 79 One 89 98.89 0.169 0.483 0.348 AG2 0.383 0.173 80 0 90 100 0.133 0.511 0.356 AG2 0.405 0.197 81 0 90 100 0.244 0.589 0.167 AG2 0.434 0.278 82 One 89 98.89 0.225 0.483 0.292 AG2 0.369 0.173 83 0 90 100 0.211 0.578 0.211 AG2 0.423 0.265 84 0 90 100 0.222 0.544 0.233 AG2 0.400 0.229 85 0 90 100 0.244 0.544 0.211 AG2 0.401 0.229 86 0 90 100 0.367 0.467 0.167 AG2 0.380 0.160 87 0 90 100 0.244 0.522 0.233 AG2 0.387 0.208 88 0 90 100 0.211 0.489 0.300 AG2 0.374 0.178 89 0 90 100 0.211 0.500 0.289 AG2 0.378 0.188 90 0 90 100 0.222 0.544 0.233 AG2 0.400 0.229 91 49 41 45.55 1.00 0 0 AG2 92 49 41 45.55 1.00 0 0 AG2

In Table 1, the number represents the sequence number on the sequence list, and rs_number represents a reference sequence, which is a single sequence in the database of the National Institute of Biotechnology Information (NCBI) under the National Institute of Health, after the Human Genome Project. Name given to distinguish nucleotide polymorphism.

The chromosome represents the number of the chromosome in which the single nucleotide polymorphism is located.

allele_A and allele_B arbitrarily named alleles for convenience of experiments in genotyping, and Freq_A and Freq_B represent the frequencies of alleles A and B, respectively. AA, AB and BB are the number of subjects with genotypes, and AA_freq, AB_freq, and BB_freq indicate their frequency.

total is the total number of subjects analyzed, fail is the number of subjects with no clear genotyping results for each SNP, and call rate is the percentage of subjects with genotyping results detected for each SNP. Indicates.

The panel is an assay group consisting of 46 SNPs, and genotyping primers using a genotyping primer at the same time by pooling the product obtained by performing PCR using PCR primers for each SNP. This represents a possible group. PI and PE represent the pair probability and the negative power for each SNP and are calculated using the following formula.

Where Gi is the fraction of the sample having genotype "i" and n is the number of markers.

Negative exclusion force (PE) = h ² (1-2 * h * H ² )

In the above, H and h means homozygotes and heterozygotes, respectively.

The paired probability of the markers selected above was 0.345 to 0.465, and the cumulative paired probability of 88 personal identification markers (SEQ ID NOs: 1 to 45 and SEQ ID NOs: 48 to 90) was calculated to be 3.61E-37.

Table 2 below shows the cumulative pair probability and the average negative exclusion power of the personal identification test using the kit currently used in the National Institute of Forensic Investigation and the kit including the polynucleotide marker according to one embodiment of the present invention.

Table 2. Comparison of Pair Probability and Average Declining Power of Individual Identification

Inspection tool Inspection items Cumulative Pair Probability (PI _T ) Mean Booking Exclusion (MEC _T ) Noodles Kit 17 STR markers 4.9E-20 99.9998% Panel 1 * 45 SNP Markers 2.04E-19 99.9930% Panel 2 * 43 SNP Markers 1.77E-18 99.9905% DL Kit * 88 SNP Markers 3.61E-37 99.99999933%

* Panels 1 and 2 consist of SNP markers labeled AG1 and AG2 in Table 1, respectively, and the DL kit consists of 88 SNP markers of Panel 1 (AG1) and Panel 2 (AG2).

The 17 STR markers used in kits currently used in Korean fruit trees are subject to the National Institute of Scientific Investigations, in accordance with the Act on the Protection and Support of Missing Children, etc. (Law No. 7560, 31 May 2005, 1 December 2005). 17 STR markers (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, Penta D, Penta E, TH01, TPOX, VWA, D2SD2S1338, D19S433).

As a criterion for personal identification, the purpose of SNP marker analysis, the general international guidelines (SWGDAM, AABB guideline) were applied. As can be seen in Table 2, the cumulative pair probability and the average derogatory power of the personal identification SNP markers selected in the present embodiment, in particular, the DL kit composed of 88 SNP markers are higher than those of the markers currently used. Confirmed that it is excellent.

Example 2 Verification of Discrimination of Personal Identification Markers

(1) genotyping through polarization detection

Of the markers selected in Example 1, 88 individual identification SNP markers (SEQ ID NOs: 1 to 45 and SEQ ID NOs: 48 to 90) and 4 gender identification SNP markers (SEQ ID NOs: 46, 47, 91, and 92) were used. Genotyping was performed on 90 individual samples, 75 families of three families, and 165 people in total.

In addition, three households consisting of three parents and children were reconfirmed the genotyping results by varying the DNA concentration.

Genomic DNA was extracted from 5 ml of blood using a commercially available DNA separation kit (Gentra Genomic DNA purification kit, Minneapolis, MN, USA) following the protocol provided by the manufacturer. The DNA of the confirmation of the genotype of the SNP site is GenomeLab ^TM SNPstream ^® system; was carried out using (Ultra-high throughput UHT system) . The system utilizes tag array single base extension gene identification techniques (Beckman Coulter, Fullerton, CA, USA) and thus SNPstream software in connection with multiplex PCR.

The genotype analysis method of the SNP region using the UHT system is summarized as follows. First, sequences surrounding the 92 SNP sites shown in Table 1 from genomic DNA (10 ng-48plex criteria) were amplified by PCR. PCR was performed simultaneously in each well of 384 well plates using the PCR primer pairs shown in Table 3 as primers, genomic DNA as template and Taq Gold polymerase as polymerase. The thermocycle was repeated 30 times at 94 ° C, 1 minute 30 seconds at 55 ° C, and 1 minute 30 seconds at 72 ° C. Exonucleases were used to remove unreacted PCR primers and dNTPs.

Next, a single base primer extension reaction (also called an SNP-IT reaction) was performed using a primer annealing to the SNP to the side (hereinafter also referred to as "SNP-IT primer"). The sequence of the SNP-IT primer for each SNP is shown in Table 2. SNP-IT primers comprise a "tag" sequence at the 5 'end of the primer. This tag sequence is annealed to the complementary probe nucleic acid (hereinafter referred to as the "tag probe") immobilized on the solid phase at the end of the multiplex SNP-IT analysis. Next, a single base extension reaction was performed in each well of a 384 well plate in the presence of the otherwise fluorescently labeled acyclonucleotide, SNP-IT primer and DNA polymerase. The thermocycle was repeated 45 times at 94 ° C. for 20 seconds and at 40 ° C. for 11 seconds.

Table 3. PCR primers and SNP-IT primers for genotyping of SNP sites

order
number rs number PCR primers
(SEQ ID NO) PCR primers
(SEQ ID NO) SNP-IT Primer
(SEQ ID NO) One rs515603 SEQ ID NO: 93 SEQ ID NO: 94 SEQ ID NO: 95 2 rs10785736 SEQ ID NO: 96 SEQ ID NO: 97 SEQ ID NO: 98 3 rs10920379 SEQ ID NO: 99 SEQ ID NO: 100 SEQ ID NO: 101 4 rs7527185 SEQ ID NO: 102 SEQ ID NO: 103 SEQ ID NO: 104 5 rs902329 SEQ ID NO: 105 SEQ ID NO: 106 SEQ ID NO: 107 6 rs10926595 SEQ ID NO: 108 SEQ ID NO: 109 SEQ ID NO: 110 7 rs10884796 SEQ ID NO: 111 SEQ ID NO: 112 SEQ ID NO: 113 8 rs12286769 SEQ ID NO: 114 SEQ ID NO: 115 SEQ ID NO: 116 9 rs10791403 SEQ ID NO: 117 SEQ ID NO: 118 SEQ ID NO: 119 10 rs1487602 SEQ ID NO: 120 SEQ ID NO: 121 SEQ ID NO: 122 11 rs12876644 SEQ ID NO: 123 SEQ ID NO: 124 SEQ ID NO: 125 12 rs12585235 SEQ ID NO: 126 SEQ ID NO: 127 SEQ ID NO: 128 13 rs9540071 SEQ ID NO: 129 SEQ ID NO: 130 SEQ ID NO: 131 14 rs9523953 SEQ ID NO: 132 SEQ ID NO: 133 SEQ ID NO: 134 15 rs2402341 SEQ ID NO: 135 SEQ ID NO: 136 SEQ ID NO: 137 16 rs4906699 SEQ ID NO: 138 SEQ ID NO: 139 SEQ ID NO: 140 17 rs691 SEQ ID NO: 141 SEQ ID NO: 142 SEQ ID NO: 143 18 rs424165 SEQ ID NO: 144 SEQ ID NO: 145 SEQ ID NO: 146 19 rs1812521 SEQ ID NO: 147 SEQ ID NO: 148 SEQ ID NO: 149 20 rs4407150 SEQ ID NO: 150 SEQ ID NO: 151 SEQ ID NO: 152 21 rs12965342 SEQ ID NO: 153 SEQ ID NO: 154 SEQ ID NO: 155 22 rs1943338 SEQ ID NO: 156 SEQ ID NO: 157 SEQ ID NO: 158 23 rs1563172 SEQ ID NO: 159 SEQ ID NO: 160 SEQ ID NO: 161 24 rs1427446 SEQ ID NO: 162 SEQ ID NO: 163 SEQ ID NO: 164 25 rs2241225 SEQ ID NO: 165 SEQ ID NO: 166 SEQ ID NO: 167 26 rs6744937 SEQ ID NO: 168 SEQ ID NO: 169 SEQ ID NO: 170 27 rs6442180 SEQ ID NO: 171 SEQ ID NO: 172 SEQ ID NO: 173 28 rs6549566 SEQ ID NO: 174 SEQ ID NO: 175 SEQ ID NO: 176 29 rs1709915 SEQ ID NO: 177 SEQ ID NO: 178 SEQ ID NO: 179 30 rs6447614 SEQ ID NO: 180 SEQ ID NO: 181 SEQ ID NO: 182 31 rs970022 SEQ ID NO: 183 SEQ ID NO: 184 SEQ ID NO: 185 32 rs2613019 SEQ ID NO: 186 SEQ ID NO: 187 SEQ ID NO: 188 33 rs4501321 SEQ ID NO: 189 SEQ ID NO: 190 SEQ ID NO: 191 34 rs10075131 SEQ ID NO: 192 SEQ ID NO: 193 SEQ ID NO: 194 35 rs11952230 SEQ ID NO: 195 SEQ ID NO: 196 SEQ ID NO: 197 36 rs7707575 SEQ ID NO: 198 SEQ ID NO: 199 SEQ ID NO: 200 37 rs1883181 SEQ ID NO: 201 SEQ ID NO: 202 SEQ ID NO: 203 38 rs6900780 SEQ ID NO: 204 SEQ ID NO: 205 SEQ ID NO: 206 39 rs2736966 SEQ ID NO: 207 SEQ ID NO: 208 SEQ ID NO: 209 40 rs6947796 SEQ ID NO: 210 SEQ ID NO: 211 SEQ ID NO: 212 41 rs624667 SEQ ID NO: 213 SEQ ID NO: 214 SEQ ID NO: 215 42 rs12546986 SEQ ID NO: 216 SEQ ID NO: 217 SEQ ID NO: 218 43 rs1875067 SEQ ID NO: 219 SEQ ID NO: 220 SEQ ID NO: 221 44 rs4876357 SEQ ID NO: 222 SEQ ID NO: 223 SEQ ID NO: 224 45 rs10811474 SEQ ID NO: 225 SEQ ID NO: 226 SEQ ID NO: 227 46 rs2444398 SEQ ID NO: 228 SEQ ID NO: 229 SEQ ID NO: 230 47 rs2521574 SEQ ID NO: 231 SEQ ID NO: 232 SEQ ID NO: 233 48 rs7921967 SEQ ID NO: 234 SEQ ID NO: 235 SEQ ID NO: 236 49 rs11599718 SEQ ID NO: 237 SEQ ID NO: 238 SEQ ID NO: 239 50 rs7298551 SEQ ID NO: 240 SEQ ID NO: 241 SEQ ID NO: 242 51 rs1166235 SEQ ID NO: 243 SEQ ID NO: 244 SEQ ID NO: 245 52 rs12820285 SEQ ID NO: 246 SEQ ID NO: 247 SEQ ID NO: 248 53 rs11060771 SEQ ID NO: 249 SEQ ID NO: 250 SEQ ID NO: 251 54 rs2039623 SEQ ID NO: 252 SEQ ID NO: 253 SEQ ID NO: 254  55 rs9523089 SEQ ID NO: 255 SEQ ID NO: 256 SEQ ID NO: 257 56 rs408283 SEQ ID NO: 258 SEQ ID NO: 259 SEQ ID NO: 260 57 rs7160372 SEQ ID NO: 261 SEQ ID NO: 262 SEQ ID NO: 263 58 rs11634785 SEQ ID NO: 264 SEQ ID NO: 265 SEQ ID NO: 266 59 rs31042 SEQ ID NO: 267 SEQ ID NO: 268 SEQ ID NO: 269 60 rs11076262 SEQ ID NO: 270 SEQ ID NO: 271 SEQ ID NO: 272 61 rs7192715 SEQ ID NO: 273 SEQ ID NO: 274 SEQ ID NO: 275 62 rs8058011 SEQ ID NO: 276 SEQ ID NO: 277 SEQ ID NO: 278 63 rs2470209 SEQ ID NO: 279 SEQ ID NO: 280 SEQ ID NO: 281 64 rs12607426 SEQ ID NO: 282 SEQ ID NO: 283 SEQ ID NO: 284 65 rs6507244 SEQ ID NO: 285 SEQ ID NO: 286 SEQ ID NO: 287 66 rs4926111 SEQ ID NO: 288 SEQ ID NO: 289 SEQ ID NO: 290 67 rs8102497 SEQ ID NO: 291 SEQ ID NO: 292 SEQ ID NO: 293 68 rs4027132 SEQ ID NO: 294 SEQ ID NO: 295 SEQ ID NO: 296 69 rs330625 SEQ ID NO: 297 SEQ ID NO: 298 SEQ ID NO: 299 70 rs6711114 SEQ ID NO: 300 SEQ ID NO: 301 SEQ ID NO: 302 71 rs4349283 SEQ ID NO: 303 SEQ ID NO: 304 SEQ ID NO: 305 72 rs12613725 SEQ ID NO: 306 SEQ ID NO: 307 SEQ ID NO: 308 73 rs3761161 SEQ ID NO: 309 SEQ ID NO: 310 SEQ ID NO: 311 74 rs6014763 SEQ ID NO: 312 SEQ ID NO: 313 SEQ ID NO: 314 75 rs6006426 SEQ ID NO: 315 SEQ ID NO: 316 SEQ ID NO: 317 76 rs7288176 SEQ ID NO: 318 SEQ ID NO: 319 SEQ ID NO: 320 77 rs12695977 SEQ ID NO: 321 SEQ ID NO: 322 SEQ ID NO: 323 78 rs2127657 SEQ ID NO: 324 SEQ ID NO: 325 SEQ ID NO: 326 79 rs4141387 SEQ ID NO: 327 SEQ ID NO: 328 SEQ ID NO: 329 80 rs6850503 SEQ ID NO: 330 SEQ ID NO: 331 SEQ ID NO: 332 81 rs2642781 SEQ ID NO: 333 SEQ ID NO: 334 SEQ ID NO: 335 82 rs6596805 SEQ ID NO: 336 SEQ ID NO: 337 SEQ ID NO: 338 83 rs1321167 SEQ ID NO: 339 SEQ ID NO: 340 SEQ ID NO: 341 84 rs9371835 SEQ ID NO: 342 SEQ ID NO: 343 SEQ ID NO: 344 85 rs11971741 SEQ ID NO: 345 SEQ ID NO: 346 SEQ ID NO: 347 86 rs4722616 SEQ ID NO: 348 SEQ ID NO: 349 SEQ ID NO: 350 87 rs7792547 SEQ ID NO: 351 SEQ ID NO: 352 SEQ ID NO: 353 88 rs2468177 SEQ ID NO: 354 SEQ ID NO: 355 SEQ ID NO: 356 89 rs7038176 SEQ ID NO: 357 SEQ ID NO: 358 SEQ ID NO: 359 90 rs7027501 SEQ ID NO: 360 SEQ ID NO: 361 SEQ ID NO: 362 91 rs2032654 SEQ ID NO: 363 SEQ ID NO: 364 SEQ ID NO: 365 92 rs9341273 SEQ ID NO: 366 SEQ ID NO: 367 SEQ ID NO: 368

Next, included a 384-well and added to the product of the single base extension reaction in each well of a plate, which SNPstream ^® Tag Array Hybridization. Hybridization was achieved by incubating at 42 ° C. for about 2 hours in a perspiration incubator. The SNPstream ^® Array Tag, and the plate surface 52 delimited area in the each well of the segment region has a tag probe is fixed that comprises a tag sequence. Four of these have tag sequences that are complementary to control sequences, and 48 have tag sequences that are complementary to 12 single base extension reaction products. Each SNP is distinguished by well location.

Next, the hybridization results SNPstream ^® UHT Array Imager using the ^TM the SNPstream ^® Array Tag plate was added to the excitation light to the segment regions 52 to the surface of each well of a measure the radiation coming out from it. The measured light intensity was stored in SNPstream ^® UHT computer, and genotyping results for each SNP of each sample were confirmed.

(2) Determination of family relations (father, mother, lost child, etc.)

The SNP marker first limited its scope to family relationships between vertical generations where common alleles existed in Mendelian genetic law. As for the standard, the 19.9% of the American Association of Blood Bank's guidelines, 99.9%, are used as the primary criteria for the three-person test. In addition, more than 99.99% is applied as a secondary standard in cases of missing persons, missing children, and mass disaster cases that require family relationship verification in a database of more than 1,000 people.

Table 4 shows the analysis results using the SNP markers selected in Example 1 for three or more families using 75 households of 35 households consisting of three parents, parents, and children. Probability of discrimination (POD), Probability of Exclusion (POE), and Probability of Paternity (POP): Probability of not being excluded because of paternity versus genetic testing As a result, the probability of not being excluded is assumed to be 50% prior to the Korean population).

Table 4. Family Relationship Determination of Three-person Family

Fam ID No. of Share allele Marker POD POE POP Family-1 0 One 0.999999997 100 Family-2 0 One 0.999999993 100 Family-3 0 One 0.999999997 100 Family-5 0 One 0.999999997 100 Family-6 0 One 0.999999996 100 Family-7 0 One 0.999999997 100 Family-8 0 One 0.999999996 100 Family-11 0 One 0.999999995 100 Family-12 0 One 0.999999997 100 Family-13 0 One 0.999999997 100 Family-14 0 One 0.999999997 100 Family-15 0 One 0.999999997 100 Family-16 0 One 0.999999997 100 Family-17 0 One 0.999999997 99.99999 Family-20 0 One 0.999999997 100 Family-23 0 One 0.999999997 100 Family-24 0 One 0.999999997 100 Family-25 0 One 0.999999997 100 Family-26 0 One 0.999999997 100 Family-27 0 One 0.999999997 100 Family-28 0 One 0.999999996 100 Family-29 0 One 0.999999995 100 Family-30 0 One 0.999999994 99.99988 Family-31 0 One 0.999999997 100 Family-32 0 One 0.999999997 100

In 35 households consisting of three parents, three parents, two parents using two children were shown in Table 5, and as expected, the number of paternity indexes decreased slightly compared to that of three children. However, all show accurate results exceeding the standard.

Table 5. Determination of Sub-Child Family Relations

Fam ID No. of Share allele Marker POD POE POP Family-1 0 One 0.999999997 99.99998 Family-2 0 One 0.999999995 99.99974 Family-3 0 One 0.999999997 100 Family-5 0 One 0.999999997 100 Family-6 0 One 0.999999997 100 Family-7 0 One 0.999999997 99.99917 Family-8 0 One 0.999999996 99.99997 Family-11 0 One 0.999999996 99.99997 Family-12 0 One 0.999999997 99.99999 Family-13 0 One 0.999999997 99.9999 Family-14 0 One 0.999999997 99.99998 Family-15 0 One 0.999999997 100 Family-16 0 One 0.999999997 99.99962 Family-17 0 One 0.999999997 99.99989 Family-20 0 One 0.999999997 99.99998 Family-23 0 One 0.999999997 100 Family-24 0 One 0.999999997 99.99935 Family-25 0 One 0.999999997 99.99993 Family-26 0 One 0.999999997 99.99961 Family-27 0 One 0.999999997 99.99996 Family-28 0 One 0.999999997 100 Family-29 0 One 0.999999995 99.99997 Family-30 0 One 0.999999994 99.91293 Family-31 0 One 0.999999997 99.99723 Family-32 0 One 0.999999997 99.99984

Nos. In Tables 4 and 5 above. of Share allele Marker refers to the number of genotypes that cannot be detected by heredity in family relationships.

(3) Confirmation of family relations exclusion

Eight pairs of samples obtained from four families composed of single parent or flagella were used for SNP markers obtained in Example 1 (Panel 1 (SEQ ID NOs: 1 to 47) and Panel 2 (SEQ ID NOs: 48 to 92)). This study examined whether it is possible to discriminate non-family relationships.

As shown in Table 6 below, in the four families, there are 8 to 12 genotypes (mismatched markers) that cannot be detected by genetic law in the family relationship, and thus the biological family is detected. Could not be confirmed.

Table 7. Family Relationship Exclusion

part
character
Matched
Number of markers panel 1 panel 2 Total discrepancy
Number of markers
Total number of markers Inconsistency
Number of markers Inconsistency
Number of markers 1B-17 8191-F 76 5 7 12 88 1A-M 5623-F 80 3 5 8 88 1A-M 1727-M 76 8 4 12 88 1A-M 4404-M 76 6 6 12 88

(4) Gender determination using SNP marker

The determination of sex was based on the principle that SNP analysis of chromosome Y confirmed male and SNPs located on chromosomes X and Y always showed heterozygote in male and homozygote in female. Determine.

If SEQID 46 is AG, 47 is GG, 91 is AA, 92 is AA, the result is male. As a result of the analysis of the 77 males and 88 females, it was confirmed that all of them were judged correctly.

<110> DNA LINK, INC.          NISI <120> Single nucleotide polymorphism marker for personal identification          and its use <160> 368 <170> KopatentIn 1.71 <210> 1 <211> 200 <212> DNA <213> Homo sapiens <400> 1 tttcctccac ctctttccaa ctccaactca aagtttctct ctcacacaag aaagcacatg 60 aggtgtttat cagttgatat tagtagttgc aaatctgcag gagctgttaa tgcacccagc 120 actctaattc cagagcatcg gacagtccct gtgtctccaa tgacatagct gactggcact 180 gggccctggc tgccccggag 200 <210> 2 <211> 200 <212> DNA <213> Homo sapiens <400> 2 tacttcaaat aacatctaca ctttttaaag aagaagattc aatctcagag aaactggttt 60 ggtttctcag ctgggaatat ttatttggtc atactaaaca ttgagccagt ggatcagcag 120 tagctgattg caagattctt aagtagacac acattacatt tcgtagggga tcaaaatatg 180 tcattctcaa gtatgctaat 200 <210> 3 <211> 200 <212> DNA <213> Homo sapiens <400> 3 aatgcttaat tgactaacag aattacaact ctaaaataat tgtagaaatt gtgttttctc 60 ggccatctgc tctctttaca ttaccacagt taggcatctt ctgtctgcac ctcacactcc 120 acacagcatt tagggcatgc actgcatcta aattatcacc taattagaga attagcctga 180 ggttacatag tatgaatcaa 200 <210> 4 <211> 200 <212> DNA <213> Homo sapiens <400> 4 tcaaaataag actgtgtcca cagattatcc ctgctcctat tttaagattg gaatctaata 60 aacagcacag gctgctctac catcatttgc tgccttacca ttgtttgtat ctatttttct 120 gttactattt ttggctatgc ttttagccac aggattgagt ctgaaagcag gcagatatgg 180 actcaagaaa gataaatcat 200 <210> 5 <211> 190 <212> DNA <213> Homo sapiens <400> 5 gttatttaga aaactgaaag gtgttctaat atcaattgaa attatcattt gttctcttac 60 aaatacattt taagaaatga ttttgatcaa tatagactga ttggttattc tgccccaatt 120 catattggga ttatattttc taatttggat atttacagta atcacagtag tgatgaggta 180 ggtcaaggca 190 <210> 6 <211> 200 <212> DNA <213> Homo sapiens <400> 6 gggaaggcat ctcctctggg aatccagaga aggcagagaa ggtagaggaa ggctgtaatt 60 ttgagcactg gggcaggacc aggagtgact tggcttctta cacacttttc taaaagttta 120 atattatttc caaattaaaa tttttttaat tatgaagcat tcagaggtaa tcacatccat 180 cactaattat tattattttt 200 <210> 7 <211> 190 <212> DNA <213> Homo sapiens <400> 7 ttaccttcag ttgtactcac tcaaatcttt cctaagttac cctgatcaga cagcttgcag 60 ccagatcatg aggtacatgt aggacatggc atgaagtttt gtttcattct gaaccaagga 120 aagccactga ggaattctga gctgcagaaa gtcactttga ctgatttatg gaaagtggat 180 tgaaggggaa 190 <210> 8 <211> 200 <212> DNA <213> Homo sapiens <400> 8 cagggagagg ggagccctgt ttggaactga agcatatcca cttaggataa gggtgacaac 60 atgtataaga ggacattctt ttgtgtattc aagaaatagt agcaagtgcc tatatgggat 120 atagttgtgg tgaagacaca aaagagtcct ttcatcatgg agcttccacg cgtccatccc 180 aaagctaagt cctggagaaa 200 <210> 9 <211> 200 <212> DNA <213> Homo sapiens <400> 9 gctgcgctct ccacaatgag gccttttcga ctgactcctg cagggtgcat gtggaccttt 60 ccctttggtt tgcatagccc tgggaggcta tttattttca tctcgaagcc attttctgta 120 ccttcaccac cagtttacac ttcaggaaac taagacattg aaagctaagt gagctgacca 180 aggctgcatt ttgagaaatg 200 <210> 10 <211> 200 <212> DNA <213> Homo sapiens <400> 10 gaaagtgcct agacggaaac atctttatag ctctgtaatg taaataatct taaagtcaaa 60 atttcaacaa cataaagcaa tgtaaatggg agactgtgca gaacgggatg gagaagagat 120 tttagctgca cccagttgta caaggatctg gctaatagtg caagcctcag cccacctaga 180 aagagaggac cgaagaaggg 200 <210> 11 <211> 200 <212> DNA <213> Homo sapiens <400> 11 tcgctaggtc ttatcttctc tggttttagc ttttctaggt aatacccaaa tgcatttgac 60 ttatggaccg tgccccacca cccttctagg gaatctccga gtaaattgtg tctgcagctt 120 aacaacgatt aatgactttc cacttataag taacagtttt attttgattc attttaacaa 180 atatatattg gcacttaaca 200 <210> 12 <211> 200 <212> DNA <213> Homo sapiens <400> 12 aagaaacagt gctcaagtaa aattgcagag aaatggtgta atttattata ttcctgccgg 60 acgttaatga tgtgctagca agtaatagca cttttactca ggcagatgaa gataccagtc 120 taagattacc ggtgggaaga aaacatgatg aaaaggtcta tttcaaaata acatatccaa 180 attagaaatc ctttcagtga 200 <210> 13 <211> 200 <212> DNA <213> Homo sapiens <400> 13 atacaaatat tttatttggt cttaccttcc tcttaaactt ttacaaactt ctttgctaaa 60 atgccagtag caacacttaa ctaaaatctc agatattttg gctagtcact tttattgata 120 ccactgcaaa gattaaagag gactttattg agattagtac tttgggaact gacttctatt 180 tatttattgc tttctagagt 200 <210> 14 <211> 200 <212> DNA <213> Homo sapiens <400> 14 aaagaaagag acagacaggg agggagaatg aatgattggt cttctgattt atctctttct 60 ctcttttatt gctggtttat gagttgaagt ctggctggga atatctgatt agtggtgttc 120 tggctacatg cctatgcctt agctaggaga aagaccggaa ctacaagtac ctaccatttt 180 cagccacaac aggtaaggaa 200 <210> 15 <211> 200 <212> DNA <213> Homo sapiens <400> 15 ataggcgtga gccaccgcgc ccagcctagc ccttgcttta aaggattgtt ctaatgactg 60 aaagacataa ttcagaaccc ctttcacaca gtacccgtca ataataggct ccataaatgt 120 tagctttctc tcttaatggt gaattgaggt aacttttcga ggcatgtaga tagtgagttg 180 ctttaaccag agcagttttt 200 <210> 16 <211> 200 <212> DNA <213> Homo sapiens <400> 16 gatcttggga gatttttgga gatggcaaag tgcttctaaa catgtttggc atggtctcct 60 tcaggctggt ggatacctca ggatggtaag tattggtttt caaaggcacc taatgttcca 120 tgtttgcagg tatgaggact gtgcatggat caatgatgac ttccatacgt gggttccttg 180 gaaagttgaa caaaatgagt 200 <210> 17 <211> 200 <212> DNA <213> Homo sapiens <400> 17 tacatgcatt cttttagtgg atagatgcac acaaacacac aagccattat ggggaaggat 60 ccacgtgtgt ggccatattg taacacattt ttctgcaaat acctctttca tttaacagcc 120 cttattcaat ggcctttttc tttttcagta gtacatacac atctgtgtca tttgttgaat 180 gacgacatga atgttttgta 200 <210> 18 <211> 200 <212> DNA <213> Homo sapiens <400> 18 aagggcttat tactgggcta catagatttc gaatccttta aaaaattaca taggcatatt 60 tttaaaagac atttaagaaa agaaatgaag atctatgaca aaaaggcttc gtcttcctca 120 cctgggacta tggagcataa acgctgcttg ccgcagcctc tctggctggc aagtgtagcc 180 tcgggctggc cctaagggcc 200 <210> 19 <211> 200 <212> DNA <213> Homo sapiens <400> 19 tgataagttc cccccattac catgcttcta attgggatga gagggggagc catttgtgca 60 aacccccaga aagctggagt ggggttggga tccaggaaag gtcaaatcca actattgacc 120 actctctgaa atgggtggga tccttagtaa tggtgttttt aactccaaag caacagggaa 180 actctccctt acagactcct 200 <210> 20 <211> 200 <212> DNA <213> Homo sapiens <400> 20 gtgaagggtt gagcacgttc catcttttca gagaacaaag ctagaaaaca tgtattttaa 60 taaaataata ttcatcatga attcatgatg tttcctattc aattcaagta gaggactttt 120 ttcacagtgt ccttttccct gtcaaatttc aaattttatt gacatgaata taattattca 180 tttgctgtat tcataataca 200 <210> 21 <211> 200 <212> DNA <213> Homo sapiens <400> 21 aagaccaaaa tgcagctgcc ttcatcaggc cctacttcca gagatgcacg ggtggatgga 60 gcttttccct ctgacccagt ccttcaactg catgtgatgc tttgggaccc acgcattcca 120 tcctttctgc cctgtccttc tcctggatgt catacattgg gatccaggtg ttcagttttc 180 tctgccttgt tcttgctctg 200 <210> 22 <211> 200 <212> DNA <213> Homo sapiens <400> 22 cctaggaggt tctccagtca actttgccat ttgaaatcat actccttaca ggcaaccaat 60 ggtgctcaaa gcatctcttt cagcatcaat cttcctactt gtgatatttg ggggatgtct 120 taattagatc agttactttg ccattccttt aagtattata tctcaagtta cctgttggct 180 tcttacaggt ctcatgaaga 200 <210> 23 <211> 150 <212> DNA <213> Homo sapiens <400> 23 tgcaccacac tgcagtacta ggcatcattt tacacctaag caaaagatca cagaacatca 60 ctaatggatg aacaaaaaaa aaaacctcca ataaaagcac tcttatttcc aggatcacaa 120 gaaataccaa gtggctaggt tgtcacctac 150 <210> 24 <211> 200 <212> DNA <213> Homo sapiens <400> 24 cactcagaaa tggtttagat ttctaaaggg aagaatagag aatcacctgg agagtaacag 60 gcagttaatc tggaataaat gaagaagact atctgataca tggctgtagg gatgtggaga 120 tttttttcta tacgtagact gtaagagtac ttggctgttt caccagtctt gcctctctac 180 ctgcattaac tctcatcctt 200 <210> 25 <211> 200 <212> DNA <213> Homo sapiens <400> 25 attttcccat ctcttttagc ttacatctgc actgtgtgat ttgataggga agcaccagag 60 aagcagcaat gggatttggg atatttgctt atgtagctta ttctagcttt gctcatgcct 120 cattctggat gttccacttc catcttaaaa tgaattgctt ctggacctcc ctttttgtat 180 gatttaactc aaaatcagta 200 <210> 26 <211> 200 <212> DNA <213> Homo sapiens <400> 26 gattgttcca gtaattaatc taagtaaaaa cgtgtccagg cctgcaatca ctcaaacatt 60 ccactccaag atattctatg ctggacagga gtcaaactca tttggaataa tgcagtcagt 120 ttcagaagtt ttaaacgtgc aaaagtccac agcctacaaa agctaattta aatccagatg 180 ccttatgtaa ataatttatt 200 <210> 27 <211> 200 <212> DNA <213> Homo sapiens <400> 27 ggagctggga ttctgcgcaa taagacccca tctgcctgac tccagcagga ggcaagacag 60 ggaggtagca cgctgagaac cctctcccca tatccacttc ttaccacctt cagcctcccc 120 tacaaatgat gcctgtgtgt ccccctcacc caccttagcc ggacacctcc ttcccttggg 180 cccaccctgc ccagcccctc 200 <210> 28 <211> 200 <212> DNA <213> Homo sapiens <400> 28 tgtctgggac tctgaaaacc tgatcattct tcttttacta atcactccat tctcaaaaat 60 caacattgtt tttggttagg tccttgtatg acagctggat gtgcattctc ttatcattag 120 ctttaagact tatccctgtt ctttgcacac atgataaaca acatttttaa taacaatttc 180 cagtgaacga atgtgaccaa 200 <210> 29 <211> 200 <212> DNA <213> Homo sapiens <400> 29 agtatgtaca gaaagtagag ggctgtgatt aacatgttaa gtcccataag aatgatggtg 60 agattggatc aagagaataa ggctatggat atgaagagtt cgctactaaa ttaatggtag 120 ggggtagggc aaggttggtg aggctagcta aaagtcatca catggccatt agtgggagta 180 gtgtctgaag gccttgagta 200 <210> 30 <211> 200 <212> DNA <213> Homo sapiens <400> 30 tatgtcataa tattaacctc ccttccatct gcttttgctg tttagtagtc tcatgtcttc 60 ttgagctaga ctatcttatg taaattcatg ttatgtgaat gaggtctcag taataacagc 120 cttctgtctt tcctcaccgt aacaggagtc ataggaaagg gggtgtgata gtctcagaaa 180 cagcctagca tctcatacaa 200 <210> 31 <211> 200 <212> DNA <213> Homo sapiens <400> 31 ttactcaagg caaaagaaag cattagagtc agaaataagg ctggactcca gtggaatgcc 60 ttgttctgca atcaggccca gcttatgaac tgcctactga ttaggaagat gaagagagtc 120 cagacaatca ctattattct ccccatatca ttaactggaa gtaaattagg tcagctcttg 180 caggaagtat tcattttttc 200 <210> 32 <211> 200 <212> DNA <213> Homo sapiens <400> 32 ttgagtgtct tttcctgatc tataaagtag tctctctctg tctctgtgga tatttcttgc 60 acaacctttc tcccttggtc ctatatgtgt atatatgatg gggagttttg acagagcatg 120 ttaggggaca acatcaagtc agggtatttc ttaaatgtct ggtctgactg ataaaaatat 180 tgtgatatta tcctataact 200 <210> 33 <211> 200 <212> DNA <213> Homo sapiens <400> 33 gtacatgtgc aggttttgtt atataggtaa actcatgtca tgggggtttg ttaattaaag 60 aagagtgtgc acatgatcca tctctagttg cttttcaaca agattcagga taaagatcaa 120 gtttccagga ctgactacat atgtgcttat cctttgggaa catgggaaag gaactctaat 180 gatgccaaat attcatagag 200 <210> 34 <211> 200 <212> DNA <213> Homo sapiens <400> 34 gaggagtggg aaggaaaatt aaaaaaaaaa aatgatactg cctagctaca agctgcagag 60 ggaaattaat attcaaaagg ttaaggtgtc tgtgaaatgt tgtaatgagt aacactcata 120 gtttgttact ataggttcat tgcctctaat gttgaggacg gcacatggat aatttacgat 180 tcccattgtc ccctctgcag 200 <210> 35 <211> 200 <212> DNA <213> Homo sapiens <400> 35 aattcctgct tcatttcatc atgttttttg cctcacaaac tacaattctt attcccaaat 60 tcaaattttt tcttgagtga tagttgtctt tctcttcttt tgcacaccca aagatctcaa 120 gtaggatatt tggggactga tgaaggggag aactcaagga aatgattggg gcctcaaaga 180 agcttctagg ccaggtgtgg 200 <210> 36 <211> 200 <212> DNA <213> Homo sapiens <400> 36 tgcacttttc aaagattttc agaccttgat acttccaagg cttattctat agggtttttg 60 tacttaatat aatacaattt tacaaggctt agaggcaaac gttagaaaca tatattaagc 120 agaaaatagg aatacttgta tcttttttct atgtgttagt tttaccctct aactagatag 180 ctccttggca tcttgaagtt 200 <210> 37 <211> 200 <212> DNA <213> Homo sapiens <400> 37 atgtctgttg ggacaaaaca tggccagttg agtgcactgt tctagattgc caactcctta 60 agagacaagg tatcttttca cccttgcttt tcctatagtg cttgcacagt atttctgaac 120 caatcagatg ttgcttaaag gaaaataaat aaataaatgg caatagcatt tataagcagg 180 tattcctaca gctttatgcc 200 <210> 38 <211> 200 <212> DNA <213> Homo sapiens <400> 38 ttttagagta ggatatagaa ccactaatac attaatcttg actttaacat ttcctcaaac 60 tctcgaaaag tatttatcct gtgtgtttga ttttagagcg ttcacctgat tctcctttag 120 gagttgataa tgcaaaacag tgaaatttat gtaattgtaa aggtagaaaa cactccttca 180 tattccatat catttctaga 200 <210> 39 <211> 200 <212> DNA <213> Homo sapiens <400> 39 gaagggaact ttgcacccca agggcatggt gtggagtcaa tagtggcaag ttcagtctgg 60 ttttttacat cagccggttc agaggaagag tgaaatcccc agatcagcag ggcaaaatgc 120 tggaagttgt cgaggatgca gagagtagca ggaagacacc tctgagaact cactcacaca 180 ccctgcccct gtgtagagat 200 <210> 40 <211> 200 <212> DNA <213> Homo sapiens <400> 40 tcagaatccc aggattaaaa agaaagagag gaaggaagga aggaaggaaa gaagaaaagg 60 aaggagggag gaaagaaggg agaatggtca aaatatatga agatgaggtc cagaatatgc 120 aaatttgttg cacgttttca gccactggta gaatatgaga aaagagaatc tgtatgtgag 180 catttaaaat atacatttga 200 <210> 41 <211> 193 <212> DNA <213> Homo sapiens <400> 41 aaattagcac caagaacaag aaatacagtc tttgaaaatt agaaattgga acttactaga 60 gcataagtgg ctgttttaaa atgtttctgg tggcacttgt tattgttacc atgtttttaa 120 cctacatcat tggggtttga tcactttcta acatcatcat ctctgaaaca cacattttct 180 agtaattata aaa 193 <210> 42 <211> 200 <212> DNA <213> Homo sapiens <400> 42 tgtcatagaa ctttagatgt catttgtcat ttatttaaag gacaaaactg aaggaagtta 60 ggttaggttt aggaatcatc aaaagaaaag aaagtgtcag ataaggagca tcacaggttc 120 ctatatccta taagttataa tatacattgg agatcagaac tttttctttt ctatttctaa 180 tatttagatt acagatgagc 200 <210> 43 <211> 200 <212> DNA <213> Homo sapiens <400> 43 taatggcgag ggtccagcta tgcctctctt tggtttgttg acagtggttc acgttcctaa 60 gcatctttct ggatgggtca tctccatttg tgtccttttg gcatcttgtt ggcttctgct 120 ccttttctac gctgtgctcc ttcctgtgga gccacaggaa ctgctgaagt tccctcccac 180 acctttgaag gctacaagga 200 <210> 44 <211> 200 <212> DNA <213> Homo sapiens <400> 44 ttcttaattt aaggtgtgaa tataaaataa aaaggcagag cttcttagaa aagagtttca 60 aaggcaattc cctaaacatc ttgaaaaata gcagcagaat gacctcagtt caggatttta 120 aagaataaca ttcattgaga catagacata attgttcagg tgattatttt tcattagtta 180 aaaaaaatgg caaatccatc 200 <210> 45 <211> 200 <212> DNA <213> Homo sapiens <400> 45 tatagcactt attagactta attctctctg tcctaacaca cacgtgagca cgcatgcaca 60 catgcacaca cacacacaca cacacacaca tgctttttac aaatatgaca acctgtatca 120 atacaggctg caaggtaatt attgcgtcaa tactattata tctcacaaag tacaagtaaa 180 gattaaacaa tcaggcctca 200 <210> 46 <211> 200 <212> DNA <213> Homo sapiens <400> 46 tttctaaagt ggctctccca aacctacctt tgtcctaact cagttgtctg tgattctcaa 60 tatagtaacg ataagcctct ttgaatatgg aggccgctgc gacggcccgg ctgagaccca 120 aagcccggtg gaaaaagaca gcccggcgaa gacccaaagc ccagcccaag acacctcaat 180 catgtcgaga aataacgcag 200 <210> 47 <211> 200 <212> DNA <213> Homo sapiens <400> 47 gaaactacaa cggcaagatt aaagatcaga ttcctttaaa aaaagtgtgc ctcccaatct 60 tacctccagt tcatttagcc tttggacgcg aggagtaaat tgaaattgtc aacttctact 120 gcaaaaggag gctgccaatc ctagaagaaa gtgtaaggac ctggtaacta tggcacagta 180 aaggatgaag atagtgaaaa 200 <210> 48 <211> 199 <212> DNA <213> Homo sapiens <400> 48 agtgtgattg caatcaagaa agttacaaaa ctttattggc atataaaatg tacacaaaaa 60 ataacataca catttgaaca atatcatatt cctgaatata aaatgactgt tgtgagaata 120 tcagttgttc ctagattaat gcacacattt ggtgtaggtc atttcaatat cctcctggct 180 attttagaaa cagaacaaa 199 <210> 49 <211> 200 <212> DNA <213> Homo sapiens <400> 49 ctttatcaca gaaaaccagt tgttccagtc agacccatac ctggaatttt taccaaagcc 60 tctctgttaa tggcatccac cagggaggac gctggtgtcc gtttaagcaa gcacttagct 120 ttgcttccac gtggcagtac ctgtatataa gaattcactg aagtgtcaaa caaatgctcc 180 caagtctaca gttcacatat 200 <210> 50 <211> 200 <212> DNA <213> Homo sapiens <400> 50 ctcttggctg gatgcatttg tgaaaagtac aatctgcaaa atcatacaat tgattttgga 60 gagttcatcg gatttttctg aatcttgaat acagtattac ctggactctt tttttggtgt 120 tggagggcag tatctgccag gctcacaatg aagtcattaa ttttgttatt acatctcttc 180 caccaccact tcccacccct 200 <210> 51 <211> 200 <212> DNA <213> Homo sapiens <400> 51 aacgtgaaat acattatgct tccattacac tagttatcat gggaaaacac ctcaaaaaaa 60 taaaaacaga ccaacaaaag acaataaaga cctttgtgcc tccattattt ttctgtccta 120 gtatttgtgg ccgggtcaaa aacaaactag acagggaccc tgagggaggg agatttcgct 180 gacatctgta ggtaggatca 200 <210> 52 <211> 200 <212> DNA <213> Homo sapiens <400> 52 gtttcctttc ttcctagtta aaaatgcacc atctctctaa ggaggtgcct gttcattggg 60 tgttcttccc caggaatgac gtttcctgtt ttatgatggg aaacaagacg ttcacactcc 120 tgagattctg ggcatcaggt ctacatctct gtcatcatcc tggccagcgt ctaccttgcg 180 aaatgatcaa aatgatgacc 200 <210> 53 <211> 200 <212> DNA <213> Homo sapiens <400> 53 ctatccaacc tgcctcgcca agataaaggc cagtgagacg tgatactggc acacttgctg 60 ctgtagcaat gtttttctgt tttgctctcc tcattgaggc aaagttttaa aattatcctt 120 actgtttttg ttgttggcct gaaaggttaa aattcatttg ggtgagcttc caacaatgac 180 tggtttctct gcagccaata 200 <210> 54 <211> 193 <212> DNA <213> Homo sapiens <400> 54 ggtccttcag gctaacattt ctttagttta cagttctacc aattagcatc attaaattac 60 catttatgtt tccttaaaaa gtggagattg catctttttt ggagatttgg ttctaaagtc 120 agtatgtgag gcaaagagtg aaagcctctt atcttatcct aaagtagaca gcagtatatg 180 tggtttgtgt aca 193 <210> 55 <211> 200 <212> DNA <213> Homo sapiens <400> 55 ttcacatggt ttaaaaagtg gaaactctga aagtgtttat aatttaaata aaatgtttat 60 ttctgtagtc aaaaggctag tgaaatttgg agacagattt ttaagcaata ttctaaattt 120 aatgttactg taaagagcaa tctgggaata tttacttctt tccagctatt gatagaaata 180 ggccacaaag agaaatatat 200 <210> 56 <211> 200 <212> DNA <213> Homo sapiens <400> 56 ctcagtatct gttctgttcc caatggtctt tagcaagagt tgcaaagcgc cctcctgtta 60 cagtaaagaa ttctccttta tatcaaatct aaattacttc gtattaggca gaaccctttt 120 aattgcaagt aacagaaact atacaaatta gcttaagcat aaagggactt tattggcttg 180 tgttcctgga tagagctggc 200 <210> 57 <211> 200 <212> DNA <213> Homo sapiens <400> 57 caggcttaca ttcagtaaga ttcatcaggc ttactattct ctctgccacc tcacactcct 60 tttcttgggt acctgttcct gtgggagaca agtattcttt agtgagtgat actggagcgt 120 tcttttcttc ctctactccc ttgctgccac ccaccaacct gccccaactt ctgaaattcc 180 catcctgggg gaggttccca 200 <210> 58 <211> 200 <212> DNA <213> Homo sapiens <400> 58 gtggaaataa cagagattag tacatggaga actatggcca aaaaaaaaaa aaaaaaaaat 60 cctgccagaa tgtattctta acttcgaaca aatgccagaa tattggtacc tttggcttcc 120 tcctcagtgc ttgtgcctat cagaacagat aaaacatgct gttttggtta gttccctacg 180 ggtctctctg tccctccggg 200 <210> 59 <211> 196 <212> DNA <213> Homo sapiens <400> 59 actgttacct caacaaataa ctaatgtaaa aagcaaagga cgtcatttga gttattgaag 60 ttcagaagta tttcaggaca catttaccag tgagttaggc tccgcactct tgggaaacaa 120 tgcccaggta attattttaa atgagcatta attatgtcat tattagaaat ggggagaaaa 180 tgcaatttcc ttctgt 196 <210> 60 <211> 200 <212> DNA <213> Homo sapiens <400> 60 agcatgcagg gcctctgctg ggtttcttaa aggagaagac cagaaatagc atccattctg 60 ctgcatttta ttggtcaagg aaagtcacgg tgccagtctc agtcaagaga aaaggtaatg 120 aacttaatgg gtgaagtggc acacacttat aggcaaggag gggattgaca gcaacccttt 180 ttggaaagta tctcccacag 200 <210> 61 <211> 200 <212> DNA <213> Homo sapiens <400> 61 taagtcttta atcaatttgg ggttgtgttt tgcattgtat atagtgtgaa gggtcctcca 60 tggtgaaggc ttctggggtc ttcctgctct cctcttaccc gcacaaagaa gtcacagttg 120 tgatgtgtct tcttggcacc cagaagatga tgcagactaa atgctttcta catttttctg 180 tgtggcttct ttggtctttg 200 <210> 62 <211> 200 <212> DNA <213> Homo sapiens <400> 62 tagatatttt tacaagtata ttacctccta tttgcataat gccttctagt tctcaaagac 60 tttttacaca cttcatcggt ttgatcttta ccatgacacc taatggaaat attatcacca 120 tcttgatttt acagatcagg aaaccaaggc tgaggaaagg gaatgacttc ctcaggaccg 180 caagatgttc agtttctcag 200 <210> 63 <211> 200 <212> DNA <213> Homo sapiens <400> 63 ctcaattgcc agatgcatat gccctgtgca catgcctttt cctctaggga aaccctgttc 60 agggtctcaa tgtcccctgg cccagctgcc ttaacttagc caaatgcaaa cttgccctgt 120 atctccagtg gctcagaact gccctgctag ctctcaaccc tcctccacgc tttttctttt 180 tccttgcttc ctggtcctgc 200 <210> 64 <211> 200 <212> DNA <213> Homo sapiens <400> 64 aaactcaatt ttcatttcaa tatccttgaa gtagaaaagt agtctttgaa caaatcactg 60 tatcttagag gcatgtgttt aaaatactgg cctagctacc agaactttta aagattgaca 120 tttccatgat gtgcctgaca atttaatcga ccttataaag tgcttctcag gcttcctggg 180 acaatgtttc ccaaagtgta 200 <210> 65 <211> 200 <212> DNA <213> Homo sapiens <400> 65 ctggtaaaac cccgagaaaa attaagaaaa agatggagag atgtagcatc aagtattctg 60 tagggatgaa ccaaggtcat tttttgaggt cttttggtct ttggtctatt tattggggat 120 tcctaacttg caagtttctt aagtgtagca tttaacacat tccataattt aactctttca 180 taaatttcca tgctacttac 200 <210> 66 <211> 200 <212> DNA <213> Homo sapiens <400> 66 ctcactctgg acaaaaccac tattatgacc agaatgcagc atcactgggc agctatgacc 60 tatatttgaa atacattctt agaaaggttc catttggaag gagtgcaacg tgcacagcac 120 ttttggggag caacttggca gagtgcatta aaacatgaag tgcaggccga gtgcaatggc 180 tcatgtctgt aatcccagca 200 <210> 67 <211> 200 <212> DNA <213> Homo sapiens <400> 67 aaggtgttga acagtaaaca cagtatactt catttattaa ataaaaagag aaacacactg 60 tatacatatt tgcttctata tgcatggaat aacactggaa gatattcaca aaactattga 120 cattggttat cttttttttt ttctttttct gactcaaaca catcttctga gacactggtt 180 atctatagag agggaaagat 200 <210> 68 <211> 200 <212> DNA <213> Homo sapiens <400> 68 cctttaattg taatgtaagt ctatcaccat ctggctgcct ctccttccat tctttatggt 60 tagctgttta tgctctggct atgacaaatt caaagttcaa tcgtatttct ggctaattct 120 tttttttttt ttttaaacat ttatttagga ggacttgaga agaaagagtg ggtactttta 180 aagtctagag tggtcagaac 200 <210> 69 <211> 200 <212> DNA <213> Homo sapiens <400> 69 tacaagtatg aagcaccaat tctcttagaa agaataatct ggctgcatgg tatcaatgac 60 acctctttta taattaaatc tataattttt tttgaatacc tctaggtccc atccttcacc 120 accatggatg catttgtgtc taatattagt gattgtttca acagaatttc aggccttttt 180 tcctgatctc tcttcctccc 200 <210> 70 <211> 200 <212> DNA <213> Homo sapiens <400> 70 tggaaataaa cagttttggg gaagatttaa tccctcactg gggaaaaaac aagcagataa 60 aatcagggtc ctctaactgt ccataatctg caacaagtac attttaattc tctgcataaa 120 atttttttat ttttaaaaaa gtaggacttc aagtgtggag atatggaatt cctcaatccc 180 tgcagcccgc cttaaagaca 200 <210> 71 <211> 200 <212> DNA <213> Homo sapiens <400> 71 tagaggatac cttgtgccat cctggttcct tcatacactg gaggtggcac cgcagtctaa 60 agagctatta tgccagctca cgtgtgtggc ttaagcccag aatcggcaga gtcctgaatt 120 gttctgtgtc cctccaaagt cagggtcact gtcaccctaa gacagcatgt cagcaccatt 180 ccagaggccc aattttatcc 200 <210> 72 <211> 200 <212> DNA <213> Homo sapiens <400> 72 tgccccattg tttgtctggt atagagctct cagcaaatgt gttttcctca gaaattgcca 60 actttgctcc acactgcatt tggaatttgg tattggagac agaattccga tgtcagtcca 120 cttctttttc ccttgtaggc aaacggttta ttgtgtttgt cagtttggaa aacacatttt 180 atcctgagaa atcaaatgta 200 <210> 73 <211> 200 <212> DNA <213> Homo sapiens <400> 73 ggcaccccaa aagtcagtgt cttcaaatag cagccattat tagttctcaa ggttacaggt 60 cagtgtgtga cccttttgcc ctgggccctt ctctgttgtt tcagctgggc tcatatggga 120 gtcattggtc aactagagga gtgctgctcc aggatggtct tgcccacatg tatggtggtc 180 agctggctgt tttggggggg 200 <210> 74 <211> 200 <212> DNA <213> Homo sapiens <400> 74 aggcactgca ccaggatttg actattgatg catctccaag ctgagcctcg gtaggcctgt 60 ggtcccatgc tagagagcag ctgccagtgc ctccatctgc gcaaagcttg gctgtgtgtg 120 gatgggtggg acaagtcact ccagaagctt ctccccttaa gacatcctga cattaaggga 180 tcttcttcct ttgctcctct 200 <210> 75 <211> 200 <212> DNA <213> Homo sapiens <400> 75 ggcagagccc cttagcggct ctaagcacta aaatactatg ctaactaaaa actaccctca 60 gcatcaaaca aagataagga agtctagtct gacttttctc tgatgacaac ataccatgcc 120 tattttgaaa cgtcagagtc tggccaaaaa tgtttgggtg aatggggtac cagtcatcat 180 tatcgggaat atttctggag 200 <210> 76 <211> 200 <212> DNA <213> Homo sapiens <400> 76 tattagatgc tgtcaaagtc taagcgaccc ttcacatttc gcattccccc gagtagtgtt 60 cagggtttac ttggtgcaca cttggccaac acgggatgtc ttctatttct agccactctg 120 gtgagtcatc agtggtgcac accatggttt caagttcatt tgcactttct cagtgtctat 180 aaaaattgag caactagcag 200 <210> 77 <211> 200 <212> DNA <213> Homo sapiens <400> 77 gaaaactgac aaggacacat tagagctgca cttcactagc agcatgaatg tggtaatggt 60 gaaaggagaa caatatctga aaggaaatga aggtcttatc taggagccac agagatttca 120 tttttgtttt tctgaaaatt atcattatca gctgtgactg ctgatgaagg accacttagc 180 attcccccca aggaataatt 200 <210> 78 <211> 199 <212> DNA <213> Homo sapiens <400> 78 taaacagact cagcctcata gggcctttcc tgagctcagg ccattcaaac ctgagcaagg 60 cgttttctca tgagcattgc cagctgagtt tcacaaaaat tattacactc tgttcttccc 120 accatgagaa catattcagt tattggattg gaaataaaga ctccagctcc ctcagctgag 180 ctgctgatgt ttgaatgtg 199 <210> 79 <211> 200 <212> DNA <213> Homo sapiens <400> 79 gcaatgcact ccaggcctta ttcaatcagg ctgtgtctca tttccttttg cacaaacctt 60 tggggaaatg tgctcgttct gaatctggtc tctataaccc ttaatgtccc tcaagggaat 120 agggatgtta ggtctctata ctgcagcgtg cacgagcaac actagcttct gctctctaag 180 cccagctctt tgccaacatg 200 <210> 80 <211> 200 <212> DNA <213> Homo sapiens <400> 80 cttttgaggt ataaataaaa tattttaaat taagcaaaca ttttaaactt tagattaaac 60 ctcattgagc atggagtaac atacatgtca gtttcaggct ataagaggca gaggcatttg 120 gaaaaacagc aaattagcat ttgtttaacc accacttttg gcacctactt tattttggtc 180 aaggtgcaaa aagttaagac 200 <210> 81 <211> 200 <212> DNA <213> Homo sapiens <400> 81 cacctgaata ttttagttaa aaagtaaaag agtagttgtc ttttaatatt agtgcttatt 60 tgagtttaga tattatttta taacttacat tgtacctctc tcagcgaaat agtttgaaac 120 tgtctcactg taagtgtata tattcttttt tcaaaatatg tacatgtagt attgtcattc 180 tgtatattaa gtattaagaa 200 <210> 82 <211> 200 <212> DNA <213> Homo sapiens <400> 82 ggcttttaaa aatagtaaaa gataaagaga acatcgaaaa ggacctacaa tttgctataa 60 ttgacatcag catcagggta aaccctgcag ccctcaaacc gttactgctg aattgtgcat 120 tccccatggg atcccacact gcttccagtg ccacagccag atccacacta aacccttaac 180 acatggaaat ggaatgccag 200 <210> 83 <211> 198 <212> DNA <213> Homo sapiens <400> 83 ataacactaa ttttagtcct gtgtgaaatt cctcaaaatt tgtgattaaa taacatgcag 60 tacaacccat ttgttcttca gccaacatat accaagtgta attgtgtgct aggcattgtg 120 caatgtgcgc tcttgccctc atggatctga cagtcaagtg ggcatgacag tcgaatcagg 180 gaactgccca ggagagaa 198 <210> 84 <211> 200 <212> DNA <213> Homo sapiens <400> 84 atgtggtttc tttccctgag acaattgagt tgttcatcta tttctagctt gttggaaaga 60 atgataccac ctcatactca gagataaggc tattgtgttc tctgtttttc cagggtgcgg 120 tgggtctgcc cagggctttg tgaacaggat ctgcctggca gataaatccg aggagtattg 180 ggaagtggac tctttgacaa 200 <210> 85 <211> 200 <212> DNA <213> Homo sapiens <400> 85 aaaaacaaag ggaaaaaaaa aaagacccaa atacttggag actgaattta attaaaatgg 60 gattgtaatg gccttaaaca tgcatgctca tgacctcatg tgaatcttcc acctctctag 120 attgtcacca tgcgtaggtt cctgagctgg tgttgctgca attttggggc atctctaggg 180 gccatgaggg ccccatgagt 200 <210> 86 <211> 200 <212> DNA <213> Homo sapiens <400> 86 ttcctggcag tgaaggtgtt ggggttaatg atgaagacca gggaaaaggg gttcacagaa 60 ttggctgagg gcttatgtgt gcagaggtct tctggaaggc tccatcagta atggatgaaa 120 gagcttatca gatcattagc agcgccccat actaatgaga tgttgcattc ctgctcagta 180 gagaccccct ccattggaaa 200 <210> 87 <211> 200 <212> DNA <213> Homo sapiens <400> 87 tatacccacc ttgttatatg taaaaaatgg gttagcacac agtttgtgtc atagtaatta 60 cccaagaaaa tgctgttggt attggtatct actgataacc gtcattgcca ggtcccgctt 120 tcatgaccca attaggttca cttaaaagta gcaatatact catacgtaaa ccagaaatgt 180 taaaatctcc tcccccacaa 200 <210> 88 <211> 200 <212> DNA <213> Homo sapiens <400> 88 tgcatattta taaataagca cagggtacta cagaagtaca tggtgaggaa ggcacttggg 60 tcagccatag gagattcagc agagacttcc tggaagaagc acttccaaac ttggatgtaa 120 aatagaagta ggagtttgag ggtaaatagg gtgcatagag aaaaacattg cagataggat 180 gcatgccatt tacaaagatc 200 <210> 89 <211> 200 <212> DNA <213> Homo sapiens <400> 89 tataccaaaa tcagttgtga aaaaaactca taaattttca aaaaaagtta gatactcaaa 60 acttatcaca taatatctaa acttctcttt cacattaatc tataaaattc aagtgaatgc 120 gtgtcagttg cattatgcca aaatgacttt tagcatgcaa ggatttttga accatttgcc 180 gttttctgga catggactta 200 <210> 90 <211> 199 <212> DNA <213> Homo sapiens <400> 90 gaaagtgaaa ttgctcatag gaaatcaaag caaatggcct taagagtggc tccagtggac 60 cgtcctctga aaagtgaccc acaattctgc atgaatcttc caggacactg aattggagcc 120 agctgatgta aggagcttac cttggttgcc accttctggt ctctgtttga ggaacaactg 180 catgtgatta agggtaaca 199 <210> 91 <211> 198 <212> DNA <213> Homo sapiens <400> 91 tctaaaatga tactgtagta aagaaatatt ctcaaactgt tggtaaattt tagagaaaat 60 aaaaatatta tacatacttg ctgcattaag acaaactgct ttctaactgt tccagctgat 120 gcttctgtgc tggatttaaa ttatctctat ttgctcgcag ttgttccaag tgctagaaga 180 aaagagatta atataatc 198 <210> 92 <211> 200 <212> DNA <213> Homo sapiens <400> 92 ttcccggaga gtatcgccag ccaaccaggc gggtgatgga ggtgcgtacc tgtccatgcc 60 accaagcgcc tccctttcct cgactgtcag gctaacagac sytcttcact ctcgcggctc 120 gcttttcctt ccgccatttt ctttgcctca tcaccgaagg caacagcggc ggtagtgagc 180 gacactgcgc aggatttcat 200 <210> 93 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 93 tcaaagtttc tctctcacac aaga 24 <210> 94 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 94 tatgtcattg gagacacagg g 21 <210> 95 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 95 aataagctca ccaccgtcaa agagtgctgg gtgcattaac agctc 45 <210> 96 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 96 tggtttggtt tctcagctg 19 <210> 97 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 97 cttaagaatc ttgcaatcag ctact 25 <210> 98 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 98 acagatcact caccgactaa aatatttatt tggtcatact aaaca 45 <210> 99 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 99 tttctcggcc atctgctc 18 <210> 100 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 100 agtgcatgcc ctaaatgct 19 <210> 101 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 101 ctcagactac gaatccacgt tgtgtggagt gtgaggtgca gacag 45 <210> 102 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 102 tgtccacaga ttatccctgc 20 <210> 103 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 103 taaaagcata gccaaaaata gtaaca 26 <210> 104 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 104 agaccgacaa gcaatctaca gtaacagaaa aatagataca aacaa 45 <210> 105 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 105 ttgttattta gaaaactgaa aggtgtt 27 <210> 106 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 106 ccaaattaga aaatataatc ccaatatg 28 <210> 107 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 107 cagaatagcc acgcctagat atatgaattg gggcagaata accaa 45 <210> 108 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 108 taattttgag cactggggc 19 <210> 109 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 109 atgtgattac ctctgaatgc ttc 23 <210> 110 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 110 cagccatcca ttcactatct aatattaaac ttttagaaaa gtgtg 45 <210> 111 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 111 ttaccttcag ttgtactcac tcaaat 26 <210> 112 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 112 agctcagaat tcctcagtgg 20 <210> 113 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 113 cgatcacctc actagaacaa catgtaggac atggcatgaa gtttt 45 <210> 114 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 114 tgtttggaac tgaagcatat cc 22 <210> 115 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 115 atgatgaaag gactcttttg tgtc 24 <210> 116 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 116 caacaagtaa tccgcagact actatatccc atataggcac ttgct 45 <210> 117 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 117 acctttccct ttggtttgc 19 <210> 118 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 118 taaagaaaag ccatttctca aaat 24 <210> 119 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 119 cagcactatt accatcacgt gaaggtacag aaaatggctt cgaga 45 <210> 120 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 120 atagctctgt aatgtaaata atcttaaagt ca 32 <210> 121 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 121 ttgtacaact gggtgcagc 19 <210> 122 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 122 agcaagacca cctagaccag ctaaaatctc ttctccatcc cgttc 45 <210> 123 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 123 tttgacttat ggaccgtgc 19 <210> 124 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 124 gtggaaagtc attaatcgtt gttaa 25 <210> 125 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 125 agacttctac gcaagcactg ttgttaagct gcagacacaa tttac 45 <210> 126 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 126 tgccggacgt taatgatg 18 <210> 127 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 127 ttttcttccc accggtaatc 20 <210> 128 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 128 tacaagcacg cactagacat tcttagactg gtatcttcat ctgcc 45 <210> 129 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 129 tttgctaaaa tgccagtagc a 21 <210> 130 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 130 aataaagtcc tctttaatct ttgcag 26 <210> 131 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 131 agccgaacta ccactgagta agtggtatca ataaaagtga ctagc 45 <210> 132 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 132 atttatctct ttctctcttt tattgctg 28 <210> 133 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <133> 133 aaaatggtag gtacttgtag ttccg 25 <210> 134 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 134 ctcactatct gacaagccac agccagaaca ccactaatca gatat 45 <210> 135 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 135 attgttctaa tgactgaaag acataattc 29 <210> 136 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 136 aattcaccat taagagagaa agctaa 26 <210> 137 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 137 gatccatcaa cagacatcac agctaacatt tatggagcct attat 45 <210> 138 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 138 aacatgtttg gcatggtctc 20 <139> <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 139 tcatcattga tccatgcaca 20 <210> 140 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 140 cactacatac gaccgcagaa aaacatggaa cattaggtgc ctttg 45 <210> 141 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 141 aaggatccac gtgtgtgg 18 <210> 142 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 142 aaagaaaaag gccattgaat aag 23 <210> 143 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 143 acgtaagacc actcaagacc ataagggctg ttaaatgaaa gaggt 45 <210> 144 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 144 agcgtttatg ctccatagt 19 <210> 145 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 145 gaatccttta aaaaattaca taggca 26 <210> 146 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 146 caacaagaca taacaacgca agaaaagaaa tgaagatcta tgaca 45 <210> 147 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 147 tgtgcaaacc cccagaaa 18 <210> 148 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 148 ggatcccacc catttcag 18 <210> 149 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 149 ctaactaagc tacgccgaca agagtggtca atagttggat ttgac 45 <210> 150 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 150 ttttcagaga acaaagctag aaaac 25 <210> 151 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 151 aaatttgaca gggaaaagga c 21 <210> 152 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 152 tacctatgac cagcaagcac catgaattca tgatgtttcc tattc 45 <210> 153 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 153 tggatggagc ttttccct 18 <210> 154 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 154 atcccaatgt atgacatcca g 21 <210> 155 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 155 ccgccagtaa gacctagacg aaggatggaa tgcgtgggtc ccaaa 45 <210> 156 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 156 accaatggtg ctcaaagc 18 <210> 157 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 157 tggcaaagta actgatctaa ttaaga 26 <210> 158 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 158 aacatccacg caactcatac aattaagaca tcccccaaat atcac 45 <210> 159 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 159 ttacatatca atcagcttac ctaaatg 27 <210> 160 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 160 aacatcacta atggatgaac aaaa 24 <210> 161 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 161 caacaatacg agccagcaag aaaaaaacct ccaataaaag cactc 45 <210> 162 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 162 tgaaacagcc aagtactctt acag 24 <210> 163 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 163 agtaacaggc agttaatctg gaata 25 <210> 164 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 164 tccagaatag acaacagacg taaatgaaga agactatctg ataca 45 <210> 165 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 165 cagagaagca gcaatggg 18 <210> 166 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 166 atggaagtgg aacatccaga 20 <210> 167 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 167 acaactaccg acgacaagac gaatgaggca tgagcaaagc tagaa 45 <210> 168 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 168 aatcactcaa acattccact cc 22 <210> 169 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 169 acttttgcac gtttaaaact tctg 24 <210> 170 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 170 caccgctatc aacagacttg ctgaaactga ctgcattatt ccaaa 45 <210> 171 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 171 aagacaggga ggtagcacg 19 <210> 172 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 172 gggacacaca ggcatcat 18 <210> 173 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 173 cagaacatcc tcagaagcaa ttgtagggga ggctgaaggt ggtaa 45 <210> 174 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 174 taatcactcc attctcaaaa atca 24 <175> 175 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 175 aaagaacagg gataagtctt aaagc 25 <210> 176 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 176 cgcagaagca actcacttct taaagctaat gataagagaa tgcac 45 <210> 177 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 177 attagtatgt acagaaagta gagggctg 28 <210> 178 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 178 cctcaccaac cttgcccta 19 <210> 179 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 179 aacatacaga cgcactcctc accccctacc attaatttag tagcg 45 <210> 180 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 180 tatgtcataa tattaacctc ccttcc 26 <210> 181 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 181 tgttacggtg aggaaagaca g 21 <210> 182 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 182 caagcaacga cctactacaa agaaggctgt tattactgag acctc 45 <210> 183 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 183 aatgccttgt tctgcaatca 20 <210> 184 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 184 gggagaataa tagtgattgt ctgg 24 <210> 185 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 185 cactagtcat aacgcagcct gtctggactc tcttcatctt cctaa 45 <210> 186 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 186 tcttgcacaa cctttctcc 19 <210> 187 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 187 cctgacttga tgttgtccc 19 <210> 188 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 188 gcaacataag accgctcaac cctaacatgc tctgtcaaaa ctccc 45 <210> 189 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 189 tgctctctat gaatatttgg cat 23 <210> 190 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 190 attaaagaag agtgtgcaca tg 22 <210> 191 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 191 ccataacaac ttaccagcca atccatctct agttgctttt caaca 45 <210> 192 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 192 aagctgcaga gggaaattaa tat 23 <210> 193 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 193 gcaatgaacc tatagtaaca aactatga 28 <210> 194 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 194 caagaccgca actagataca aaaggttaag gtgtctgtga aatgt 45 <210> 195 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 195 acaattctta ttcccaaatt caaa 24 <210> 196 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 196 ttcatcagtc cccaaatatc c 21 <210> 197 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 197 atctaacgca cctacgacct cctacttgag atctttgggt gtgca 45 <210> 198 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 198 aaggcttatt ctatagggtt tttgtac 27 <210> 199 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 199 aaagatacaa gtattcctat tttctgct 28 <210> 200 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 200 ccagatcctc accatgtaag tttctgctta atatatgttt ctaac 45 <210> 201 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 201 ttctagattg ccaactcctt aaga 24 <210> 202 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 202 tatttatttt cctttaagca acatctg 27 <210> 203 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 203 atacctacca cgctacagcc gattggttca gaaatactgt gcaag 45 <210> 204 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 204 atttcctcaa actctcgaaa agtat 25 <210> 205 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 205 aatttcactg ttttgcatta tcaa 24 <206> 206 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 206 accgcactaa gcaatgtatc aactcctaaa ggagaatcag gtgaa 45 <210> 207 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 207 tgtgtgagtg agttctcaga gg 22 <210> 208 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 208 tgtggagtca atagtggcaa 20 <210> 209 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 209 agtagcctaa cagcactcga ggttcagagg aagagtgaaa tcccc 45 <210> 210 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 210 attcagaatc ccaggattaa 20 <210> 211 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 211 tggctgaaaa cgtgcaac 18 <210> 212 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 212 cacgacaaga caacagatac aatttgcata ttctggacct catct 45 <210> 213 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 213 agtgatcaaa ccccaatgat 20 <210> 214 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 214 tagagcataa gtggctgttt taaa 24 <210> 215 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 215 cagtcaacaa tccagatcaa tttaaaatgt ttctggtggc acttg 45 <210> 216 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 216 aaaactgaag gaagttaggt taggtt 26 <210> 217 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 217 atattataac ttataggata taggaacctg tga 33 <210> 218 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 218 acaactcacg caagtaccat tataggaacc tgtgatgctc cttat 45 <210> 219 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 219 tttggtttgt tgacagtggt t 21 <210> 220 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 220 aacttcagca gttcctgtgg 20 <210> 221 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 221 acaatcaaca tacgaacagc aaaggagcag aagccaacaa gatgc 45 <210> 222 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 222 aaaagagttt caaaggcaat tc 22 <210> 223 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 223 gtctatgtct caatgaatgt tattcttta 29 <210> 224 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 224 gcaagccatc agctaataca ttctttaaaa tcctgaactg aggtc 45 <210> 225 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 225 ttaattctct ctgtcctaac acacac 26 <210> 226 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 226 aataattacc ttgcagcctg tatt 24 <210> 227 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 227 acaagaactc catgactcaa acacacacac acacatgctt tttac 45 <210> 228 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 228 tttgtcctaa ctcagttgtc tgtg 24 <210> 229 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 229 ggctgtcttt ttccaccg 18 <210> 230 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 230 aagtaccacg tcaacgtcac cctcttkgaa tatggaggcc gctgc 45 <210> 231 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 231 ccaatyttac ctccagttca t 21 <210> 232 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 232 taggattggc agcctcct 18 <210> 233 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 233 gcagacaacg aacaactacc tttgcagtag aagttgacaa tttca 45 <210> 234 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 234 ggcatataaa atgtacacaa aaaataac 28 <210> 235 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 235 tgcattaatc taggaacaac tgatat 26 <210> 236 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 236 caagaccgca actagataca tgaacaatat catattcctg aatat 45 <210> 237 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 237 aatttttacc aaagcctctc tgt 23 <210> 238 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 238 aatatgtgaa ctgtagactt gggag 25 <210> 239 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 239 cagccatcca ttcactatct tccaccaggg aggacrctgg tgtcc 45 <210> 240 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 240 aattgatttt ggagagttca tcg 23 <210> 241 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 241 tgagcctggc agatactgc 19 <210> 242 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 242 ctcactatct gacaagccac ttctgaatct tgaatacagt attac 45 <210> 243 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 243 acgtgaaata cattatgctt cca 23 <210> 244 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 244 tctagtttgt ttttgacccg g 21 <210> 245 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 245 caacaagtaa tccgcagact aaaagacaat aaagaccttt gtgcc 45 <210> 246 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 246 tttcttccta gttaaaaatg cacc 24 <210> 247 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 247 atgtagacct gatgcccaga 20 <210> 248 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 248 tccagaatag acaacagacg atgacgtttc ctgttttatg atggg 45 <210> 249 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 249 tgatactggc acacttgctg 20 <210> 250 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 250 agagaaacca gtcattgttg ga 22 <210> 251 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 251 cagcactatt accatcacgt tctgttttgc tctcctcatt gaggc 45 <210> 252 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 252 gctttcactc tttgcctca 19 <210> 253 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 253 ttaccattta tgtttcctta aaaagtg 27 <210> 254 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 254 caacaatacg agccagcaag atactgactt tagaaccaaa tctcc 45 <210> 255 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 255 tttaaaaagt ggaaactctg aaagt 25 <210> 256 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 256 cagattgctc tttacagtaa cattaaat 28 <210> 257 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 257 cgatcacctc actagaacaa gctagtgaaa tttggagaca gattt 45 <210> 258 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 258 gaggccagct ctatccagg 19 <210> 259 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 259 gttacagtaa agaattctcc tttatatcaa a 31 <210> 260 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 260 agaccgacaa gcaatctaca caattaaaag ggttctgcct aatac 45 <210> 261 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 261 atgaaaacca ggcttacatt ca 22 <210> 262 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 262 caagggagta gaggaagaaa aga 23 <210> 263 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 263 ccagatcctc accatgtaag aaagaacgct ccagtatcac tcact 45 <210> 264 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 264 aaataacaga gattagtaca tggagaacta t 31 <210> 265 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 265 ttttatctgt tctgataggc acaa 24 <210> 266 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 266 atctaacgca cctacgacct tcttaacttc gaacaaatgc cagaa 45 <210> 267 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 267 gtcatttgag ttattgaagt tcagaa 26 <210> 268 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 268 ttaatgctca tttaaaataa ttacctgg 28 <210> 269 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 269 gatccatcaa cagacatcac ggacacattt accagtgagt taggc 45 <210> 270 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 270 tgctgggttt cttaaaggag 20 <210> 271 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 271 ttgcctataa gtgtgtgcca 20 <210> 272 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 272 ctcagactac gaatccacgt caaggaaagt cacggtgcca gtctc 45 <210> 273 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 273 aaaatgtaga aagcatttag tctgc 25 <210> 274 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 274 ccatggtgaa ggcttctg 18 <210> 275 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 275 aagtaccacg tcaacgtcac catcacaact gtgacttctt tgtgc 45 <210> 276 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 276 ttctcaaaga ctttttacac acttc 25 <210> 277 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 277 tctgcgaatg gaaagagc 18 <210> 278 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 278 ctaactaagc tacgccgaca tcggtttgat ctttaccatg acacc 45 <210> 279 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 279 ttttcctcta gggaaaccct 20 <210> 280 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 280 ttgagagcta gcagggca 18 <210> 281 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 281 gcagacaacg aacaactacc cctggcccag ctgccttaac ttagc 45 <210> 282 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 282 ttgaagtaga aaagtagtct ttgaacaa 28 <210> 283 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 283 attaaattgt caggcacatc atg 23 <210> 284 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 284 tacaagcacg cactagacat tgtttaaaat actggcctag ctacc 45 <210> 285 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 285 tattctgtag ggatgaacca a 21 <210> 286 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 286 aatgctacac ttaagaaact tgca 24 <210> 287 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 287 cgcagaagca actcacttct gtcatttttt gaggtctttt ggtct 45 <210> 288 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 288 ggcagctatg acctatattt gaa 23 <210> 289 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 289 ttttaatgca ctctgccaag t 21 <210> 290 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 290 cagaacatcc tcagaagcaa ttcttagaaa ggttccatty ggaag 45 <210> 291 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 291 tgttgaacag taaacacagt atacttca 28 <210> 292 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 292 tgtttgagtc agaaaaagaa aaaa 24 <210> 293 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 293 acagatcact caccgactaa ctatatgcat ggaataacac tggaa 45 <210> 294 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 294 tctccttcca ttctttatgg ttag 24 <210> 295 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 295 tctagacttt aaaagtaccc actctttc 28 <210> 296 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 296 ccataacaac ttaccagcca tggctatgac aaattcaaag ttcaa 45 <210> 297 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 297 aatcactaat attagacaca aatgcatc 28 <210> 298 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 298 gctgcatggt atcaatgaca 20 <210> 299 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 299 gcaacataag accgctcaac atggtggtga aggatgggac ctaga 45 <210> 300 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 300 aagatttaat ccctcactgg g 21 <210> 301 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 301 atatctccac acttgaagtc ctactt 26 <210> 302 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 302 cagaatagcc acgcctagat actgtccata atctgcaaca agtac 45 <210> 303 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 303 cagtctaaag agctattatg cc 22 <210> 304 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 304 gtttttatat gagaccgtga aagatt 26 <210> 305 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 305 cactacatac gaccgcagaa gctcacgtgt gtggcttaag cccag 45 <210> 306 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 306 ttgccaactt tgctccac 18 <210> 307 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 307 tttgcctaca agggaaaaag a 21 <210> 308 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 308 agacttctac gcaagcactg gcatttggaa tttggtattg gagac 45 <210> 309 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 309 cagcactcct ctagttgacc 20 <210> 310 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 310 ggtcagtgtg tgaccctttt 20 <210> 311 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 311 agtagcctaa cagcactcga atgactccca tatgagccca gctga 45 <210> 312 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 312 cctgtggtcc catgctag 18 <210> 313 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 313 ttctggagtg acttgtccca 20 <210> 314 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 314 acaatcaaca tacgaacagc ccatccacac acagccaagc tttgc 45 <210> 315 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 315 taaaatacta tgctaactaa aaactaccct c 31 <210> 316 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 316 ataatgatga ctggtacccc att 23 <210> 317 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 317 caagcaacga cctactacaa aaggaagtct agtctgactt ttctc 45 <210> 318 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 318 atttttatag acactgagaa agtgca 26 <210> 319 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 319 gttcagggtt tacttggtgc 20 <210> 320 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 320 tacctatgac cagcaagcac ctcaccagag tggctagaaa tagaa 45 <210> 321 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 321 agcatgaatg tggtaatggt g 21 <210> 322 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 322 cagtcacagc tgataatgat aatttt 26 <210> 323 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 323 ccgccagtaa gacctagacg tctgaaagga aatgaaggtc ttatc 45 <210> 324 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 324 attcaaacct gagcaaggc 19 <210> 325 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 325 attcaaacat cagcagctca g 21 <210> 326 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 326 aacatacaga cgcactcctc cattgccagc tgagtttcac aaaaa 45 <210> 327 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 327 atttcctttt gcacaaacct t 21 <210> 328 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 328 ttagagagca gaagctagtg ttgc 24 <210> 329 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 329 gcaagccatc agctaataca gttctgaatc tggtctctat aaccc 45 <210> 330 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 330 ttaaacttta gattaaacct cattgagc 28 <210> 331 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 331 ttgaccaaaa taaagtaggt gcc 23 <210> 332 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 332 caccgctatc aacagacttg gtaacataca tgtcagtttc aggct 45 <210> 333 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 333 atttaaagtt cgatttaaga atcacct 27 <210> 334 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 334 aaaaaagaat atatacactt acagtgagac a 31 <210> 335 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 335 aacatccacg caactcatac ttttataact tacattgtac ctctc 45 <210> 336 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 336 aaaaggacct acaatttgct ataatt 26 <210> 337 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 337 tggaagcagt gtgggatc 18 <210> 338 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 338 cactagtcat aacgcagcct gggtaaaccc tgcagccctc aaacc 45 <210> 339 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 339 tgggcagttc cctgattc 18 <210> 340 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 340 aacatgcagt acaacccatt t 21 <210> 341 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 341 agcaagacca cctagaccag ttgcacaatg cctagcacac aatta 45 <210> 342 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 342 tttctttccc tgagacaatt ga 22 <210> 343 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 343 tgttcacaaa gccctggg 18 <210> 344 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 344 acaagaactc catgactcaa actcagagat aaggctattg tgttc 45 <210> 345 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 345 aaatgggatt gtaatggcct 20 <210> 346 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 346 aggaacctac gcatggtg 18 <210> 347 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 347 acaactcacg caagtaccat aaacatgcat gctcatgacc tcatg 45 <210> 348 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 348 ttggggttaa tgatgaagac c 21 <210> 349 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 349 aatgcaacat ctcattagta tggg 24 <210> 350 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 350 aataagctca ccaccgtcaa tgtgtgcaga ggtcttctgg aaggc 45 <210> 351 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 351 tcatagtaat tacccaagaa aatgc 25 <210> 352 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <352> 352 tatattgcta cttttaagtg aacctaattg 30 <210> 353 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotying primer <400> 353 agccgaacta ccactgagta ttggtattgg tatctactga taacc 45 <210> 354 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 354 acagtgtaat tttttgctgc atatt 25 <210> 355 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 355 tatttaccct caaactccta cttctatt 28 <210> 356 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 356 atacctacca cgctacagcc tcagcagaga cttcctggaa gaagc 45 <210> 357 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 357 gatatcatat accaaaatca gttgtga 27 <210> 358 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 358 ttttggcata atgcaactga 20 <210> 359 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 359 accgcactaa gcaatgtatc tctaaacttc tctttcacat taatc 45 <210> 360 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 360 aaatcaaagc aaatggcctt 20 <210> 361 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 361 aaggtaagct ccttacatca gct 23 <210> 362 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 362 caacaagaca taacaacgca agctggctcc aattcagtgt cctgg 45 <210> 363 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 363 caaactgttg gtaaatttta gagaaaa 27 <210> 364 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 364 atttaaatcc agcacagaag ca 22 <210> 365 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 365 acgtaagacc actcaagacc atacttgctg cattaagaca aactg 45 <210> 366 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 366 atgccaccaa gcgcctcc 18 <210> 367 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 367 aaagaaaatg gcggaagg 18 <210> 368 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> genotyping primer <400> 368 cagtcaacaa tccagatcaa aaagcgagcc gcgagagtga agars 45  

Claims (15)

In a polynucleotide selected from the group consisting of the nucleotide sequences of SEQ ID NOs: 1 to 92, or a complementary polynucleotide thereof, SNP (single nucleotide polymorphism) is present at the 101 st position of the polynucleotide, and 10 or more including the 101 st base. A personal identification polynucleotide marker consisting of polynucleotides consisting of contiguous nucleotides. In a polynucleotide selected from the group consisting of SEQ ID NOs: 46, 47, 91, and 92, or a complementary polynucleotide thereof, SNP is present at position 101 of the polynucleotide and is selected from the group consisting of 10 or more consecutive nucleotides including the 101st base. Gender identification polynucleotide marker consisting of a polynucleotide consisting of. The method of claim 1 or 2, wherein the individual is derived from a Korean, Yorba (YRI) of Ibadan, Nigeria (JPT) of Tokyo, Han Chinese (CHB) of Beijing, China, or ancestors of Northern and Western Europe. Polynucleotide marker that is a Utah resident (CEU). Personal identification microarray comprising the polynucleotide marker of claim 1. Personal identification composition comprising the polynucleotide marker of claim 1. The polynucleotide marker according to claim 5, wherein the polynucleotide marker is a combination of polynucleotides consisting of 10 or more contiguous nucleotides, including the SNP located at 101st in the polynucleotides of SEQ ID NOs: 1 to 45 and 48 to 90 or complementary polynucleotides thereof. Composition. Gender identification composition comprising the polynucleotide marker of claim 2. 8. The polynucleotide marker of claim 7 wherein the polynucleotide marker is a combination of polynucleotides consisting of 10 or more contiguous nucleotides including the SNP located at 101st in the polynucleotide of SEQ ID NOs: 46, 47, 91 and 92 or its complementary polynucleotide Composition. Composition for confirming paternity of three or more families consisting of parents, parents and children comprising the polynucleotide marker of claim 1. The polynucleotide marker according to claim 9, wherein the polynucleotide marker is a combination of polynucleotides consisting of 10 or more contiguous nucleotides including the SNP located at 101st in the polynucleotides of SEQ ID NOs: 1 to 45 and 48 to 90 or complementary polynucleotides thereof. Composition. Providing an isolated nucleic acid sample; And A method of identifying an individual comprising determining the nucleotide of the SNP position of the polynucleotide marker as defined in claim 1. 12. The polynucleotide marker according to claim 11, wherein the polynucleotide marker is a combination of polynucleotides consisting of 10 or more contiguous nucleotides, including the SNP located 101st in the polynucleotides of SEQ ID NOs: 1 to 45 and 48 to 90 or complementary polynucleotides thereof. That is. The method of claim 11, wherein determining the nucleotide at the SNP position of the polynucleotide marker is performed by sequencing, hybridization with a probe, or single base primer extension. The method of claim 11, wherein the method may further comprise comparing the nucleotides of the determined SNP positions of the polynucleotide markers with the results of the control sample. The method of claim 14, wherein the control sample is DNA of a comparison individual or DNA profile of a registered individual.