KR101731619B1 - Polynucleotide marker composition for identifying father and daughter and its use - Google Patents

Abstract

The present invention relates to a marker composition for identifying female and female relationships, a microarray containing the same, and a method for identifying a female-female relationship using a marker composition for identifying female and female relationships. According to the marker composition for confirming the female-female relationship according to one embodiment, the female-female relationship can be effectively confirmed.

Description

[0001] The present invention relates to a marker composition for identifying female and female relationships, and a polynucleotide marker composition for identifying father and daughter,

The present invention relates to a marker composition for identifying female and female relationships, a microarray containing the same, and a method for identifying a female-female relationship using a marker composition for identifying female and female relationships.

Since the development of DNA profiling and DNA fingerprinting for personal identification in 1985 by JEFFREIS in the UK, progress has been made, and through the standardization process over the last decade, the current STR gene marker analysis Has been established and used, and is now widely used in many countries due to its excellent analytical ability and discrimination. However, despite the high discriminatory power and excellent analytical ability, there is a possibility of misidentification due to the relatively high mutation rate (~ 10 ^-3 ), and in particular, the number of missing persons It is confirmed that there are some problems in the discrimination power.

As a part of efforts to solve these problems, it is possible to estimate physical characteristics such as race estimation, blood type, eye color as well as individual identification with a low mutation rate (2 to 2.5 X 10 ^-8 ) Research and development on SNP markers capable of system that can read out is progressing steadily.

Krawczak reported that the paternity exclusion power of one STR marker is similar to that of SNPs with a frequency of allelic expression of 0.5 and that of Gill et al. Indicates that 50 SNPs with allelic frequencies between 0.2 and 0.8 0.999, which is similar to 12 STRs.

In addition, Ayres et al. Suggested that 50-60 paternity indexes for family members with three SNP markers between 0.3-0.7 were needed, and 70-80 for single parent family members.

Since the female X chromosome is constituted by one female in the female relationship, one X chromosome of the female with the normal genotype is the same as the father X chromosome. Therefore, by comparing the results of X chromosome genetic tests with those of men and women,

The X chromosome is relatively small in size compared to the 22 trichomes, and therefore has relatively low genetic variation. Based on this, mutation of X chromosome has been mainly used for statistical study of human. Amanda M Casto et al. Identified the differences among the 14 ethnic groups based on the results of the 16,297 X chromosome SNPs reported in the CEPH (human genome diversity projects in the Center D'etude du Polymorphism).

To date, there have been few studies on the identification of individuals using the X chromosome or the identification of women. In order to select a valid X chromosome SNP marker in Korean, a large screening process using large-scale SNP data is required. Therefore, it is required to develop a single nucleotide polymorphic genetic marker for discriminating female females for a Korean population.

One aspect is to provide a marker composition for identifying female and female relationships.

Another aspect is to provide a microarray comprising a marker composition for identifying female and female relationships.

Another aspect is to provide a method for identifying a female-female relationship using a marker composition for identifying a female-female relationship.

In one aspect, at least one polynucleotide or a complementary polynucleotide thereof selected from the group consisting of the nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 54 has a single nucleotide polymorphism (SNP) at the 101st position of the polynucleotide, And a polynucleotide consisting of at least 10 consecutive nucleotides including a base sequence.

According to one embodiment, the marker composition may be for identifying a kinship between a father and a daughter.

The utility of a single nucleotide polymorphic marker composition used for identification of female relationships according to one embodiment can be judged by the "matching probability (PI)" and "power of exclusion".

The term "matching probability (PI)" means the probability that a particular genotype will be found by chance in any population. For a combination of several markers, the cumulative match probability is used to determine the usefulness of the marker as a marker for confirming the female relationship. A marker with a lower probability of success or a lower probability of cumulative probability is required for accurate determination of female and female relationships.

The term "Power of Exclusion (PE)" means the ability to exclude the possibility that a relative, especially a father, of a man is a biological father. Paternity exclusion depends on the genetics of both mother and child, and on the ethnic background of the mother and the presumed father.

The polynucleotide marker for identifying female partnership consisting of 10 or more consecutive nucleotides including the SNP located at position 101 of SEQ ID NO: 1 to SEQ ID NO: 54 has a minimum exclusion rate of 99.9999349% to 99.9999589% when all 54 markers are used, (Overall EP: overall exclusion probability) .

In the present invention, "polynucleotide" may be DNA or RNA. The polynucleotide may also be in single stranded or double stranded form. The polynucleotide may also be modified so that the sugar, base or phosphate site of a natural nucleotide, an analogue of a natural nucleotide, or a natural nucleotide is modified, as long as it has a property of hybridizing to a complementary nucleotide by hydrogen bonding, (Scheit, Nucleotide Analogs, John Wiley, New York (1980); Uhlman and Peyman, Chemical Reviews, 90: 543-584 (1990)), and nucleotides selected from the group consisting of . In addition, the polynucleotide may comprise a peptide nucleic acid (PNA). The polynucleotide can be detected, for example, in a manner such that a detectable label (for example, a fluorescent substance such as Cy3 or Cy5) is changed to a 3 'position in order to facilitate detection of the polynucleotide or a complex to which the polynucleotide is bound, Terminal or 5 ' end.

According to one embodiment, the polynucleotide exhibits a single base polymorphism at the SNP site. If one single-stranded polynucleotide is associated with the discrimination of the female relationship, it can be judged that the polynucleotide complementary to the single-stranded polynucleotide is naturally associated with the discrimination of the female-female relationship. Thus, a polynucleotide according to one embodiment of the invention may comprise a single-stranded polynucleotide and a polynucleotide having a complementary sequence thereof, which are associated with the discrimination of a female-female relationship with one particular sequence. For example, the polynucleotide of SEQ ID NO: 1 is the 101st (SNP position) nucleotide is "A or C ". In this case, the polynucleotide includes not only 10 or more consecutive nucleotides selected from the polynucleotide of SEQ ID NO: 1 but also the 101st (SNP position) nucleotide corresponding to the 101st (SNP position) Quot; T < / RTI > or G "nucleotides at a single site. In this regard, all sequences herein are referred to by reference to sequences on the sense strand in the genomic DNA, unless otherwise noted. In the present invention, single nucleotide polymorphism (SNP) is used as is commonly known in the art. SNPs can represent a single nucleotide polymorphism present in the genome within a population. The SNP may have a frequency of a minor allele of the SNP in the population of 1% or more.

In one embodiment of the invention, the polynucleotide may be 10-100 nucleotides in length. For example, the polynucleotide may be 10 to 50 nucleotides in length, or 10 to 30 nucleotides in length.

According to one embodiment, the polynucleotide may be a primer or a probe.

The term "primer " refers to a single-stranded polynucleotide capable of acting as a starting point in the polymerization of a nucleotide by a polymerase. For example, the primer can serve as a starting point for template-directed DNA synthesis in the presence of suitable conditions, i.e., four different nucleoside triphosphates and polymerases, at the appropriate temperature and in the appropriate buffer Lt; RTI ID = 0.0 > polynucleotides < / RTI > The appropriate length of the primer may vary depending on various factors, for example, temperature and use of the primer. The primer may be 10 to 50 nucleotides in length. In general, the shorter the length of the primer, the more stable hybrid complex can be formed with the template at lower annealing temperatures.

The sequence of the primer does not need to have a sequence completely complementary to a partial sequence of the template, and it is sufficient if the primer has complementarity within a range capable of hybridizing with the template to perform the primer-specific function. Therefore, the primer includes not only the polynucleotide itself but also a sequence that specifically hybridizes to the polynucleotide, and can act as a starting point in the polymerization reaction. For example, it may be a sequence completely complementary to the polynucleotide of SEQ ID NO: 1, or a sequence complementary to such a sequence that hybridizes to this sequence and can perform a primer action. The design of the primer can be readily carried out by those skilled in the art by reference to the sequence of the target nucleic acid to be amplified. For example, it can be designed using a commercially available program for primer design. Commercially available primer design programs include, but are not limited to, the PRIMER 3 program, for example.

According to one embodiment, when the polynucleotide is used as a PCR primer, in addition to the polynucleotide, it may include a primer that specifically binds to its complementary strand.

The term "probe" refers to a polynucleotide that specifically binds to a specific target sequence. The polynucleotide may be DNA or RNA. The polynucleotide may be in single stranded form. The polynucleotide may also be a polynucleotide which is not only composed of a natural nucleotide but also a natural nucleotide, an analogue of a natural nucleotide, a sugar, a base or a phosphate site of a natural nucleotide, which is capable of being hybridized to a complementary nucleotide by hydrogen bonding And nucleotides selected from the group consisting of combinations thereof. The polynucleotide includes PNA. The polynucleotide may be a detectable label (for example, a fluorescent substance such as Cy3 or Cy5), for example, in order to detect the polynucleotide or a complex to which the polynucleotide is bound in the assay reaction, Terminal or 5 ' end.

The probe may be an entirely complementary sequence to the polynucleotide comprising the SNP site. In addition, the probe may have a substantially complementary sequence within a range that does not interfere with specific hybridization to the polynucleotide including the SNP site. In addition, the probe may have a modified nucleotide within a range that does not impair the specific hybridization to the polynucleotide including the SNP site. Examples of such probes include a perfect probe consisting of a sequence complementary to the polynucleotide including a SNP site, and a polynucleotide comprising a SNP site, And a mismatch probe having a complementary sequence.

The primers and probes can be used to amplify or confirm the presence of nucleotide sequences with specific alleles at the SNP site.

Another aspect provides a microarray for identifying a female relationship comprising the marker composition for identifying the female relationship.

The term "microarray" in the present invention means that the polynucleotide is immobilized at a high density in the divided region of the substrate surface. The microarray may be such that the region is arranged on the substrate at a density of, for example, 400 / cm ² or more, 10 ³ / cm ² , or 10 ⁴ / cm ² .

Another embodiment of the present invention provides a kit for confirming a female relationship comprising a marker composition for identifying the female relationship.

The polynucleotide markers in the kit may be in the form of microarrays that are arranged at high density in distinct regions on the substrate surface. In one embodiment of the present invention, the polynucleotide marker comprises at least one polynucleotide selected from the group consisting of the nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 54 or a complementary polynucleotide thereof, wherein a polynucleotide having a single base Polymorphism (SNP) exists and may be a combination of polynucleotides consisting of 10 or more consecutive nucleotides, including the 101st nucleotide.

The kit may be a kit for specifically amplifying the polynucleotide and confirming the female-female relationship through the presence or absence of the amplification product. In this case, the kit may include the polynucleotide as a primer and the reagent necessary for amplification. The amplification reagent may comprise, for example, dNTPs, polymerases, and suitable buffers. For example, the primer may be one in which the nucleotide sequence corresponding to the SNP site forms the 3'-terminal nucleotide of the primer, the 3'-terminal nucleotide is complementary to the nucleotide at the SNP site (specific primer) (Non-specific primers). The non-specific primer may include a sequence that is not complementary to the 3 'terminal nucleotide as well as other sites. The kit may also include instructions for use. For example, in the case where the target sequence is amplified in the amplification reaction using the specific primer and the target sequence is not amplified in the amplification reaction using the non-specific primer, May include an explanation of the result determination, including determining that the sequence is present and identifying individuals from the results.

The kit may be a kit for hybridizing the polynucleotide or a probe derived therefrom with a nucleic acid in a sample and identifying an individual from the hybridization result. In this case, the kit may include the probe and a reagent necessary for hybridization. Reagents necessary for hybridization include, for example, a hybridization buffer. The nucleic acid may not be amplified or amplified. Thus, the kit may further comprise reagents necessary for amplification of the nucleic acid. The nucleic acid may be labeled with a detectable label. The kit may comprise a perferct match probe that is completely complementary to the polynucleotide or a mismatch probe that is complementary at all sites except for the SNP site in the polynucleotide. The probes may be in the form of microarrays fixed to a plurality of discrete regions on the substrate. The kit may also include instructions for use. For example, when the target sequence is detected in the hybridization reaction using the fully complementary probe and the target sequence is not detected in the hybridization reaction using the mismatch probe, the user's manual may include a profile obtained by each probe , And a description of the result determination, including an explanation of determining that the result is a female-female relationship.

According to one embodiment, the kit may be a kit for confirming a kinship relationship between a father and a daughter.

Yet another aspect provides a method for detecting nucleic acid, comprising: providing an isolated nucleic acid sample; And

And determining a nucleotide at a SNP position of the polynucleotide contained in the marker composition for identifying female and female relationships.

A method for identifying a female-female relationship according to one embodiment may include providing a separate nucleic acid sample. Methods for separating nucleic acids from an individual are known in the art. For example, DNA can be isolated directly from tissues or cells by amplifying specific regions by nucleic acid amplification methods such as PCR. The separated nucleic acid sample includes not only the purely isolated nucleic acid but also a cell lysate containing a crude nucleic acid, for example, a nucleic acid. The nucleic acid amplification method may include PCR, ligase chain reaction (LCR), transcription amplification, self-sustained sequence replication, and nucleic acid-based sequence amplification (NASBA). The separated nucleic acid may be DNA or RNA. The DNA may be a genomic DNA, a cDNA, or a recombinant DNA. The RNA may be mRNA.

The method may further include the step of determining the nucleotide at the SNP position of the polynucleotide contained in the marker-identifying marker composition.

According to one embodiment, the step of determining the nucleotide at the SNP position of the polynucleotide marker can be performed by sequencing, hybridization with a probe, or single base primer extension.

Methods for determining the nucleotides at SNP positions are well known in the art. For example, the nucleotide at the SNP position can be determined directly by a nucleotide sequencing method of known nucleic acids. The nucleotide sequence determination method may include a sanguine (or dideoxy) method or a curd-gilbert (chemical cleavage) method. In addition, the nucleotide at the SNP position can be determined by hybridizing a probe containing the sequence of the SNP position with the polynucleotide of interest, and analyzing the hybridization result. The degree of hybridization can be confirmed, for example, by marking a detectable label on the target nucleic acid, detecting the hybridized target nucleic acid, or confirming it by an electrical method or the like. In addition, a single base primer extension (SBE) method can be used.

In the above method, the step of determining the nucleotide at the SNP site comprises: hybridizing the nucleic acid sample to a microarray in which the polynucleotide marker is immobilized; And detecting the hybridization result.

According to one embodiment, the method for confirming the female relationship may further include comparing the nucleotide at the SNP position of the polynucleotide included in the determined female relationship confirmation marker composition with the result of the control sample. According to one embodiment, the control sample may be a DNA of an individual to be compared or a DNA profile of a registered individual.

According to one embodiment, the method for confirming the female-female relationship may further include identifying a nucleotide at a SNP position of the polynucleotide included in the determined female-female relationship-identifying marker composition and calculating a female-female relationship determination index.

It is judged that the polynucleotide marker is not a female-female relation when the polynucleotide marker which can be determined in the method of confirming the female-female relationship is definitely different even if at least one genotype exists among the samples to be compared, that is, when the nucleotide at the SNP position is different. In this case, The exponent is zero. On the other hand, when all the nucleotides at the SNP positions of the polynucleotide markers used coincide with each other, the genotyping is determined and the total EP is calculated. The calculation method of the exclusion power is as follows:

If we assume that allele a _i is located in the locus of the X chromosome in the presumptive part, the probable genotypes of the progeny are a _i a _i and a _i a _j (i ≠ j). In this case, each Exclusion probability (EP) is (1-p _i ) ² and (1-p _j ) ² . Where p _i is the allele frequency of a _i (frqeuency). The exclusion probability from Locus l is EP _l , The overall exclusion probability of combining K SNP information is as follows.

Overall EP =

According to the marker composition for confirming the female-female relationship according to one embodiment, the female-female relationship can be effectively confirmed.

Hereinafter, one or more embodiments will be described in more detail by way of examples. However, these embodiments are intended to illustrate one or more embodiments, and the scope of the invention is not limited to these embodiments.

Example  1: For checking the relationship between women and women SNP Marker  selection

(1) Subject classification

Selection of SNPs on the X chromosome was performed using the results of 8842 affymetrix 5.0 array chips from KAREI consortium. Among the 10,249 markers on the X chromosome, the Minor allele frequency was 40-50% and LD r ² > 0.3.

(2) SNP Marker  selection

The SNP markers with the Minor allele frequency of 40 to 50% were selected first in Korea, and the SNPs with PCR annealing 55 ° and SNP extension mix (CA / GT, CT / AG) Except for the AT / CG mutation and triallele resulting in chaos.) Sequence blast was used to exclude more than two products in the homology-high SNP and the designed SNP by In silico PCR. Thereafter, except for the case of r ² > 0.3, 54 SNPs without LD relation were selected including the case where the distance between two SNPs was 500 KB or more, by analyzing LDs between SNPs on the same chromosome.

A total of 54 SNPs were selected from the X chromosome based on the above criteria, and a list of selected SNPs is shown in Table 1 below

number rs_number chromosome position allele_A allele_B Freq_A Freq_B AA_freq AB_freq BB_freq One RS11091032 X 114935973 C A 0.50 0.50 0.25 0.51 0.24 2 RS11091235 X 48249579 G A 0.50 0.50 0.25 0.50 0.26 3 RS11094826 X 22223688 A G 0.50 0.50 0.25 0.50 0.25 4 RS1151983 X 144426841 A G 0.53 0.47 0.27 0.50 0.22 5 RS1157461 X 142449461 C T 0.47 0.53 0.23 0.49 0.29 6 RS1194563 X 3152515 G A 0.47 0.53 0.22 0.50 0.28 7 RS12398872 X 87570286 C A 0.49 0.51 0.24 0.51 0.25 8 RS12556842 X 117589676 T C 0.53 0.47 0.28 0.50 0.22 9 RS12558151 X 152255911 T C 0.43 0.57 0.19 0.49 0.32 10 RS12689108 X 103798511 T A 0.44 0.56 0.19 0.50 0.31 11 RS12862958 X 43288888 T C 0.51 0.49 0.25 0.52 0.23 12 RS1353456 X 78829377 A C 0.55 0.45 0.30 0.50 0.21 13 RS1409993 X 69182937 G A 0.60 0.40 0.36 0.47 0.16 14 RS1417426 X 139748649 T C 0.60 0.40 0.36 0.48 0.16 15 RS1417427 X 139764074 C G 0.53 0.47 0.27 0.51 0.22 16 RS1455393 X 108002021 C T 0.59 0.41 0.36 0.47 0.17 17 RS1538568 X 145195174 C T 0.55 0.45 0.30 0.50 0.20 18 RS1602407 X 7562529 A G 0.59 0.41 0.35 0.48 0.17 19 RS1716765 X 117329584 T C 0.58 0.42 0.34 0.49 0.18 20 RS17222293 X 9064639 A G 0.40 0.60 0.16 0.48 0.36 21 RS17280844 X 9807916 A G 0.48 0.52 0.23 0.50 0.27 22 RS17319502 X 144835250 A T 0.53 0.47 0.28 0.49 0.23 23 RS17332568 X 152720304 A G 0.59 0.41 0.35 0.48 0.16 24 RS17337757 X 95713460 A G 0.54 0.46 0.29 0.50 0.21 25 RS180497 X 150081028 C G 0.51 0.49 0.26 0.49 0.25 26 RS1918560 X 23283726 T G 0.52 0.48 0.27 0.51 0.23 27 RS198781 X 38546033 C G 0.45 0.55 0.20 0.50 0.30 28 RS209766 X 43707868 T C 0.42 0.58 0.18 0.49 0.33 29 RS2107528 X 23141280 T G 0.44 0.56 0.20 0.49 0.31 30 RS217989 X 118436563 G C 0.59 0.41 0.35 0.47 0.17 31 RS2238926 X 24132176 T A 0.47 0.53 0.22 0.50 0.27 32 RS2239425 X 9849979 A G 0.46 0.54 0.22 0.48 0.30 33 RS225049 X 29078003 C T 0.50 0.50 0.25 0.51 0.25 34 RS2285633 X 13644460 T C 0.40 0.60 0.17 0.47 0.37 35 RS2428676 X 23051183 C T 0.44 0.56 0.19 0.49 0.31 36 RS2522942 X 16474673 C T 0.59 0.41 0.35 0.48 0.17 37 RS261713 X 117873108 T C 0.55 0.45 0.30 0.50 0.20 38 RS2858769 X 47315109 C T 0.55 0.45 0.29 0.51 0.19 39 RS2980062 X 152434081 T C 0.46 0.54 0.22 0.49 0.29 40 RS3125986 X 112142357 A G 0.48 0.52 0.22 0.51 0.27 41 RS3131264 X 128605307 G T 0.50 0.50 0.25 0.50 0.25 42 RS3813932 X 118104748 A G 0.54 0.46 0.29 0.50 0.21 43 RS3827416 X 151753107 C T 0.56 0.44 0.32 0.49 0.19 44 RS3915920 X 68354065 G A 0.51 0.49 0.27 0.50 0.24 45 RS41537844 X 68530863 T C 0.54 0.46 0.30 0.49 0.21 46 RS42897 X 119476138 T C 0.60 0.40 0.36 0.48 0.16 47 RS4460557 X 29529775 A G 0.56 0.44 0.31 0.50 0.19 48 RS4474149 X 122458057 T C 0.43 0.57 0.18 0.49 0.33 49 RS4481738 X 47162675 T C 0.41 0.59 0.17 0.48 0.35 50 RS4825476 X 122269160 G A 0.41 0.59 0.16 0.49 0.35 51 RS4827139 X 39070580 T G 0.47 0.53 0.21 0.51 0.28 52 RS4829851 X 135767761 T C 0.46 0.54 0.22 0.49 0.29 53 RS4969678 X 95702574 A G 0.44 0.56 0.19 0.50 0.31 54 RS500294 X 149415656 C T 0.45 0.55 0.21 0.49 0.30

In Table 1, the numbers indicate the sequence numbers on the sequence listing, and rs_number means the reference sequence. After the human genome project, the single nucleotide sequence (SEQ ID NO: 1) in the database of the National Institute of Biotechnology Information (NCBI) This is the name given to distinguish the base polymorphism. The chromosome represents the number of the chromosome in which the single nucleotide polymorphism is located. Position indicates the position where each sequence number exists on each chromosome.

Allele_A and allele_B are randomly named alleles for ease of experiment in genotyping, and Freq_A and Freq_B represent frequencies of alleles A and B, respectively. AA, AB and BB are the number of subjects with a genotype, and AA_freq, AB_freq and BB_freq represent the frequency.

(3) Criteria for determining the relationship between women and women

If all of the alleles of the sample and the daughter of all the markers do not contain any of the father's alleles, it is a principle to judge that it is not a female-female relationship. In this case, 0, and the exclusion rate of the pseudonym is estimated as follows. If it is more than 99.99%, it is judged that the possibility of "female relationship" can not be excluded.

When we assume that allele a _i is located in the locus of the X chromosome of the presumptive part, the probable genotypes of the presumptive woman are a _i a _i and a _i a _j (i ≠ j). In this case, each Exclusion probability (EP) is (1-p _i ) ² and (1-p _j ) ² . Where p _i is the allele frequency of a _i (frqeuency). The exclusion probability of locus in EP _l , The overall exclusion probability of combining K SNP information is as follows.

Overall EP =

Example  2: Example and Results of Determining Female-Female Relationship

Table 3 shows the results of analyzing the selected markers for three pairs of female and female relationships.

number rs_number Female-female relationship 1 genotype Female relationship 2 genotype Female-female relationship 3 genotype Part 1 Girl 1 Exclusion rate 1-exclusion rate Part 2 Girl 2 Exclusion rate 1-exclusion rate Part 3 Woman 3 Exclusion rate 1-exclusion rate One RS11091032 A A C 0.250 0.750 A C A 0.250 0.750 C C A 0.250 0.750 2 RS11091235 A A A 0.250 0.750 A A A 0.250 0.750 G G A 0.250 0.750 3 RS11094826 G G G 0.250 0.750 A A G 0.250 0.750 A A G 0.250 0.750 4 RS1151983 G A G 0.221 0.779 A A G 0.281 0.719 A A A 0.221 0.779 5 RS1157461 C C C 0.281 0.719 C C T 0.221 0.779 T T T 0.221 0.779 6 RS1194563 G G A 0.221 0.779 G G G 0.281 0.719 A G A 0.281 0.719 7 RS12398872 - - A A A 0.240 0.760 A C A 0.240 0.760 8 RS12556842 T T T 0.221 0.779 T T C 0.281 0.719 T T C 0.281 0.719 9 RS12558151 C T C 0.325 0.675 T T C 0.185 0.815 T T C 0.185 0.815 10 RS12689108 A A A 0.194 0.806 T T T 0.314 0.686 T T T 0.314 0.686 11 RS12862958 T T T 0.240 0.760 C C C 0.260 0.740 C C C 0.260 0.740 12 RS1353456 A A C 0.303 0.698 C A C 0.203 0.798 A A C 0.303 0.698 13 RS1409993 A G A 0.160 0.840 G G A 0.360 0.640 A G A 0.160 0.840 14 RS1417426 T T T 0.160 0.840 C T C 0.160 0.840 T T C 0.360 0.640 15 RS1417427 G G G 0.281 0.719 C C C 0.221 0.779 C C C 0.221 0.779 16 RS1455393 T C T 0.168 0.832 T T T 0.348 0.652 T T T 0.348 0.652 17 RS1538568 T C T 0.203 0.798 T C T 0.203 0.798 C C T 0.303 0.698 18 RS1602407 A A G 0.348 0.652 G A G 0.168 0.832 G A G 0.168 0.832 19 RS1716765 T T T 0.176 0.824 - - T T T 0.176 0.824 20 RS17222293 G G G 0.160 0.840 A A A 0.360 0.640 A A A 0.360 0.640 21 RS17280844 G A G 0.270 0.730 G G G 0.230 0.770 G G G 0.230 0.770 22 RS17319502 A A T 0.281 0.719 A A T 0.281 0.719 A A T 0.281 0.719 23 RS17332568 G G G 0.348 0.652 A A G 0.348 0.652 A A A 0.168 0.832 24 RS17337757 A A G 0.292 0.708 G A G 0.212 0.788 A A A 0.212 0.788 25 RS180497 C C G 0.260 0.740 G C G 0.240 0.760 G G G 0.260 0.740 26 RS1918560 T T G 0.270 0.730 G T G 0.230 0.770 T T G 0.270 0.730 27 RS198781 C C C 0.303 0.698 C C C 0.303 0.698 G C G 0.303 0.698 28 RS209766 C C C 0.176 0.824 C C C 0.176 0.824 T T C 0.176 0.824 29 RS2107528 G T G 0.314 0.686 T T G 0.194 0.806 T T G 0.194 0.806 30 RS217989 G G C 0.348 0.652 G G C 0.348 0.652 C G C 0.168 0.832 31 RS2238926 T T A 0.221 0.779 T T T 0.281 0.719 T T T 0.281 0.719 32 RS2239425 A A G 0.212 0.788 G G G 0.212 0.788 A A G 0.212 0.788 33 RS225049 C C C 0.250 0.750 C C T 0.250 0.750 C C T 0.250 0.750 34 RS2285633 C C C 0.160 0.840 T T C 0.160 0.840 - - 35 RS2428676 C C T 0.194 0.806 T C T 0.314 0.686 C C T 0.194 0.806 36 RS2522942 C C T 0.348 0.652 C C C 0.168 0.832 C C C 0.168 0.832 37 RS261713 C C C 0.303 0.698 C T C 0.203 0.798 C C C 0.303 0.698 38 RS2858769 T C T 0.203 0.798 T C T 0.203 0.798 C C T 0.303 0.698 39 RS2980062 T T C 0.212 0.788 T T C 0.212 0.788 T T T 0.292 0.708 40 RS3125986 A A A 0.270 0.730 A A A 0.270 0.730 A A A 0.270 0.730 41 RS3131264 T T T 0.250 0.750 T T T 0.250 0.750 T T T 0.250 0.750 42 RS3813932 A A G 0.292 0.708 A A G 0.292 0.708 G A G 0.212 0.788 43 RS3827416 T C T 0.194 0.806 C C T 0.314 0.686 C C T 0.314 0.686 44 RS3915920 G G A 0.260 0.740 G G G 0.240 0.760 G G A 0.260 0.740 45 RS41537844 T T C 0.292 0.708 C C C 0.292 0.708 T T C 0.292 0.708 46 RS42897 T T T 0.160 0.840 T T C 0.360 0.640 T T C 0.360 0.640 47 RS4460557 A A A 0.194 0.806 A A G 0.314 0.686 G A G 0.194 0.806 48 RS4474149 C C C 0.185 0.815 T T C 0.185 0.815 C C C 0.185 0.815 49 RS4481738 T T C 0.168 0.832 T T T 0.348 0.652 C T C 0.348 0.652 50 RS4825476 G G A 0.168 0.832 A A A 0.168 0.832 A A A 0.168 0.832 51 RS4827139 T T G 0.221 0.779 T T G 0.221 0.779 T T G 0.221 0.779 52 RS4829851 C T C 0.292 0.708 T T C 0.212 0.788 C T C 0.292 0.708 Exclusionary power
(Overall EP) 99.9999349% 99.9999688% 99.9999648%

"-" in the above table is the case where the analysis result is not derived and is excluded from the calculation in this case.

In the case of each pair of female partnership, the analysis showed that all negative alleles were observed in the family. In this case, it was confirmed that the exclusion power for paternity was at least 99.9999349% (" ) There were many unmatched alleles among the women of other families, and it was confirmed that they were not female - female relations.

&Lt; 110 > DNA LINK, INC. <120> Single nucleotide polymorphism marker composition for          identification of father and daughter and its use <130> PN096235 <160> 54 <170> Kopatentin 1.71 <210> 1 <211> 201 <212> DNA <213> Homo sapiens <400> 1 catgccatgt tggatctggc ctaattccac ctccaataca gaggaggcat ggagatgtct 60 gggttaatgt gccaggtcat agcaccattt acagcgtttt atgagtgtac cccagcagtc 120 ctttctggca tgtagcaggc tctctccaca agaagcccaa ttggttcttc cttctgcatg 180 ctcttggctt cctggacttc a 201 <210> 2 <211> 201 <212> DNA <213> Homo sapiens <400> 2 ttttcctata caaggacact attgacatgc ccaccagggt ggctagaacc aaaaagtcag 60 ataacaagta tttgtgagga tgtggagcaa tggaaacctc atactatgct ggtagcaatg 120 taaaaaaaga aaaaaaagtc cttgctctta ggatattcat actgaagtat ttagttaaaa 180 gggtgtcata tccgcaacca c 201 <210> 3 <211> 201 <212> DNA <213> Homo sapiens <400> 3 atgtgatatat atatcaggat ttgttgagct tcctggccct ggtggcacaa aagaagcacc 60 atattactta gtgcttcatg gctagcttga aatgcaacga agatttgtcc agccaggctc 120 cacaccccca aaaagctcct ggctgtgtca tttgctcgtt tgcctttaaa ggtactaata 180 taacagcaag taccttacat g 201 <210> 4 <211> 201 <212> DNA <213> Homo sapiens <400> 4 agaacaagat gcacagaaag agcttcagag atctgcagtg tccctcttaa atattcagca 60 gagtattttg aatgcatgtg aggaaactcg cctaggtaaa agaaaggact actcaagaag 120 attaagaaca atcccaagtt tcaaacaggg ctgggtgtag tggacaaagt attctatagc 180 acacaaggta gagggttttg c 201 <210> 5 <211> 201 <212> DNA <213> Homo sapiens <400> 5 aagtgttcac atccacccgt atctgtgctg cacggttcaa atggagcaat tgcctgggtt 60 aaggcgcata atacctataa caaaggggaa gtcaggatga cgcattgaag tccacagagg 120 taggctcgag tcccagcaag gccacttgct acctatgtga cttggggcac attcactaac 180 tctcagcctg tttcctcagg a 201 <210> 6 <211> 201 <212> DNA <213> Homo sapiens <400> 6 aagacgtgtg cccactgagc ctttccaagt gctttactct gagatggtcc aagaggtgtt 60 tctgagaagg ctatttttat tgtctttggt gacacattat atgatgcaga tgtggtttcc 120 atcaatccat ctagaaagtc tatgcatagg gttatgtctc actttcccta ttagtgacct 180 ggaggaagca ctgcattttt g 201 <210> 7 <211> 201 <212> DNA <213> Homo sapiens <400> 7 acaaaaaagt aacaaaaaag taacttcttt tgatttcata ggctcacaac tggagggaca 60 tttgcttcag gatgaattat gtcttgagtc tcactgatat atgatttgga taagaccctg 120 gactttagag ttgatgctga aatgagttaa gacttctgag gctattggac tggaatggat 180 gcattttgca tgtaaggagg a 201 <210> 8 <211> 201 <212> DNA <213> Homo sapiens <400> 8 agcgaaattc cctcctcggt ctacatatta gttttgatgc ttcccatacc cattcctgtc 60 aattttgcat cctgcacagc tcaaaaacga aaccaaattt cgactgtcct ggaacatata 120 ttgtgtgggg aagacaaaga ataaacacat ataaaaagta atattagaaa gataaatgag 180 agaaagggag tatagactgt c 201 <210> 9 <211> 201 <212> DNA <213> Homo sapiens <400> 9 accccttgga agcttctgga accttccaga ggagccttat ttgggtggtc ttgctgcctt 60 tcccctgccc agggaaggac agcgcacctg ttagagaatg ctgcttggct atttgtgcga 120 taaaatcagt tgaacgctgg tctttaggct cagaagctac cgttagccag tagccaaaca 180 tccagcagtg aggcagggat g 201 <210> 10 <211> 201 <212> DNA <213> Homo sapiens <400> 10 tctctgtggg tgtgagttat gagagctgta ttgtaagtgt gctctcccta aatggttaca 60 gacaagaaac acatggtttt aagaggcacc cattaaaccc ataagatgaa tggcatgtat 120 ttggcatctc tactttgtgg agaaacaaga cagtggtttc ctgaaatcaa attctgccaa 180 ggattgtctt agaaataggg c 201 <210> 11 <211> 201 <212> DNA <213> Homo sapiens <400> 11 gagaatggca gaaggggaga gatagacagt tagaaaatat ccaagatata gaattatcca 60 gtcctcacaa ttctagctgt aggttactct tatgcagagt catgcttctt gaggatgata 120 cccagaaatg tggtcacagc tcatctagcc ctgccttctg gagaggactt catcttattt 180 taaccctcta ctaccaccat g 201 <210> 12 <211> 201 <212> DNA <213> Homo sapiens <400> 12 gttatctttg tcctgttgga aatatatata aaacaggtct agggaaacgc agctggttag 60 aatgcaaaac ccttttacaa cagagaaacg tcgctataga actgcatttt gtgagcacac 120 ggccttcatg ctaaatcctg cacttttttc ttagttaatt tcacttgtac cttggcgact 180 attatgcaga atgatccata c 201 <210> 13 <211> 201 <212> DNA <213> Homo sapiens <400> 13 tgagtaaggc tacaactaga ctttctgaat gaaatgaatg atttttctaa tactttgtgg 60 taagaaaccc accccctata tggctttagg atgcttcact atgaaatgtt aaatttgtgt 120 gtctgtttca agaccactgt actcaatttt tttttttttt ttggacaggg tctcactctg 180 tcacctaggt tggagtgcag t 201 <210> 14 <211> 201 <212> DNA <213> Homo sapiens <400> 14 ctccatctgg cccactaggc ccaaggctgc tggcttgctg ggatttgaac tcttctcttg 60 ctaatgcata taaaaacaat ttcctgctta tatcccattt cgttactttc tgaagcaagc 120 ttctgctgtg gcttataaga agttgtttta cattttgtca tacaggtata catgttgaga 180 gtggggatgg agtgatattt t 201 <210> 15 <211> 201 <212> DNA <213> Homo sapiens <400> 15 gcagaaatct aatggctgag aaagagccag ccattgaagg caggaggaat gtgggcaagc 60 taaacaggat tccaggcttt gggaacacca tagcgagaga ctcaaaggca ggaaagagcg 120 tggtgttttc caggaaatga aaggaggcaa aatgtggaag atatgaatga gggagaggga 180 caatttatta caaaacagta g 201 <210> 16 <211> 201 <212> DNA <213> Homo sapiens <400> 16 aaatggttgt gagaatctcc ttaagtccta ctcctctact aatgcagatc ctttgagctc 60 tcttttacta tttctccttt attttcctct cgatgattaa ctcaaatgcc attctgaagt 120 gtgctcaaca cataactaga tgctgaagag caggtggggg gaacttgcag aaaaagccct 180 gtaagtgtga cagtaagtga g 201 <210> 17 <211> 201 <212> DNA <213> Homo sapiens <400> 17 gccaagtctg tgaggactgg gcttgtccaa gtgtaacatt acatgtcgta ttgcattgat 60 gtcatgcagc actacaatat ggcagtttca gttatagttg caagagcata ggaatgtctt 120 ctgaagctaa tgaagtggta tttatattac ttcatgggaa cggccattaa aataattgct 180 cactatatct taaaaaatat c 201 <210> 18 <211> 201 <212> DNA <213> Homo sapiens <400> 18 ggccacatgc tcagtcataa agcaagtgtt aatgattttt aaaaaaatca aaatcatacc 60 aagcacactc ttgaaccaca gtgcaataaa accagaagtc aatatcaaga aggtctctca 120 aaactatgca aatacatgga aattaaacaa cttgttcctg aatgactcct gggtgaacat 180 caaaattaag gcagaaatca a 201 <210> 19 <211> 201 <212> DNA <213> Homo sapiens <400> 19 tatattacct tactctttca cctactttct taaggaatct ggctttggtt gaagttaagt 60 acatactgtc ttccaggtag agatatacta ctttaaagtc ctcaagtcct tgtgattcta 120 aggtcagaca ccattgacta agtaactagg caaatccaaa gactcagttt ttgacaaaca 180 gagtagtatt tgtagagatg t 201 <210> 20 <211> 201 <212> DNA <213> Homo sapiens <400> 20 tccacattgc ctgagcaaac tataagggga aattggtgtt tatttacatc ccatttctct 60 tcccaaggga tttgaaagac caggaggaag ctattaagga aacagtcgta ttttttccat 120 tgttatcctc atttatgata tagccattgc cccgtcacta aaatagctgc tgagccactt 180 ggtaaggcaa aagggaattc t 201 <210> 21 <211> 201 <212> DNA <213> Homo sapiens <400> 21 tctctggagg acctgtatgt gctatgcagt gggatccaca ctgtacaggt taagtttatt 60 ttgaggttca aggaattgag aagttagcat tagccttaac aatgaagttt aaatgacttt 120 tattttttta tcggttccac aaatgatgta gacttttatt ttttaaaaca gagcagctgt 180 aatgagatcc accaaattga a 201 <210> 22 <211> 201 <212> DNA <213> Homo sapiens <400> 22 ttaattttaa ataagtattc aaaaaattca cacacaaatt atattttttc ccaggaaaag 60 ataaatattg ccaatgtact gctatttgac ctcaaacaag acagattagg agtctcctca 120 aacaaggaag cttttgactt ggagaagtta actgttgttt ttagtttatg attgattgag 180 acaaatatat gaagatacct t 201 <210> 23 <211> 201 <212> DNA <213> Homo sapiens <400> 23 gaggccctga ccctgcccag cattgagcat ggctcctagg acttgataga gcccgaaggt 60 ttgctgagag ctcccaggat ggaagggccc tggcaacgac acggttattc atctatctgg 120 ccaagacatc cactaggtgc cttctgcaaa ctgagcacct tgctaggcac tgtgggatca 180 ccaaatgggg agggcagctt c 201 <210> 24 <211> 201 <212> DNA <213> Homo sapiens <400> 24 tctgacctca aatatgctta tatagcactg tgtgaactat gtgagtgtgt acattctcct 60 tttctcctcc ctttctctct gaaaagtgga ccagtagaac aaacagatgt ttcagagtta 120 cacagatgtg ggtttgaatc tcaattccac cacttctcag tttcttattt tctctttgtc 180 tctaaaatgg agataataac a 201 <210> 25 <211> 201 <212> DNA <213> Homo sapiens <400> 25 aaactgcact tagcacttgg caggagtggc ccttgccctg gaacctgccc tttaaggggt 60 ggcaaggctg gaggagggcc agagcaatat ctgttaaagt caatgactca tggcagggtc 120 aacgagtcca aaggatcaag aggttgcact agaccagagc tgatacctct ccccgcactt 180 ctagaccatg ctttagttgc c 201 <210> 26 <211> 201 <212> DNA <213> Homo sapiens <400> 26 gctactgcta tgttccaggt tcttctatta cattatgtct cagctttcta tgagctatgt 60 tatttacaag gtaaaagaat aacacctaag ttcatccatt gcttaagtga acccaaacgt 120 agtttgagat ctgtaggccg gtttaaattg agtctggtaa agctacttca gcctggaaaa 180 ggagagaaga aatgatagac t 201 <210> 27 <211> 201 <212> DNA <213> Homo sapiens <400> 27 atccttttgc tagatccctt ggatttccgt tttccgtgtg ttatcacact gttggaactg 60 ggaagaagag aaaaagaggc aagattttgg catttagtaa cttaagtctg aataccgagt 120 tttcatccgg gtgcatacgt acttaaaata ccaaatgggt ccaggaaggg ggacaaagta 180 cgctttaatt tatgagaaga t 201 <210> 28 <211> 201 <212> DNA <213> Homo sapiens <400> 28 acatcaaaga tttataagtg gaagaaagct tgctagataa ccctagataa aacttcatac 60 agtactagga tcagaaaagc aagaaactct aggtctaatt cagaaagtgt gaagtgttaa 120 gtagcccctt cccaggaact caaaggattc caattggatc tagtcaacta gatgccttgg 180 agaataagtc aaatgcctct t 201 <210> 29 <211> 201 <212> DNA <213> Homo sapiens <400> 29 attcatttat ggttttgctt ttcctaaata aaagcatgta tgcagagcag gcacatgtgg 60 gtagcttgtt tttactaatc tttatttaac actaaatatc ggatctgacg ttctcatata 120 ctatcaaatg tggtaaccat atcattcagc caacatagtg tcccttttcc ccaggaactg 180 ccacagaata gtattcttct t 201 <210> 30 <211> 201 <212> DNA <213> Homo sapiens <400> 30 tccctgcact gactggcccc tttgtctgga tgtttctttc tcttaatatg cttttctcta 60 gacacacaca gcccatttaa tggcacatca ggttggaact cttttgaaca taatgacagt 120 aatcagaagt cttgtttaac cagctgagcc cttccttcac catgcttaca ctcattttat 180 ccctttttcc ttttcttgcc t 201 <210> 31 <211> 201 <212> DNA <213> Homo sapiens <400> 31 tggttttgaa actgaaggta tattaaaatg tagtttgttg attagtgata tcaaaaatag 60 accaaaacaa caactgcttt cagtgaccca gagtttggga atcttgaact ttgcatttga 120 aacaagctga ttaaatttta taatttttat gtttgtaaat gacaaaggtt tggggctgcc 180 attttcaagc ttttctttgc a 201 <210> 32 <211> 201 <212> DNA <213> Homo sapiens <400> 32 actcatctgt caatgtctgc ttaggtggct tccatgcctt ggcattcaca atataaatag 60 ttcacacaag tgaactattc acaatagttc atgtcgtagt aaacatgtga gttctgccat 120 attttgcttg ttcaaaagga gtcagagtcc ggcctacttc taacaagctc cacttgtttg 180 aaggaaggag tttcaaagga a 201 <210> 33 <211> 201 <212> DNA <213> Homo sapiens <400> 33 atggcttggc atcagaagtg gaaaggaaag tagagtgttg tagaatgagt gctggatttg 60 aggtcagaag acctctttta attcccggta ctgtcaccga ctagatgtga cctatggcat 120 aacccttaaa tatccttgac ctcctctgtt tccttttatg ttaaaatggg aataataatg 180 catgctttga ccatcattta g 201 <210> 34 <211> 201 <212> DNA <213> Homo sapiens <400> 34 actctttttg tgtatgagac ttagttttaa aattagctat taaacaaaat gtttttcccc 60 cctaaaaaaa gaaaagtagc cccataaatg gaaacttaca ctttcacctg actgtgaagt 120 ctacagactc agagcctggc atctatgttc catgttcttc tggtttgtga gcccaaactt 180 tagttagtta cctttactaa g 201 <210> 35 <211> 201 <212> DNA <213> Homo sapiens <400> 35 atttcacttt cttcgtcctc tcgtcacagg tcttaacttt ctttctttgt tttcacatga 60 accctagcaa tgtcctcccc tatcctttat gttttataag caacgaatca cacagcactc 120 aattaagcat gtaagtctgt ctccctgtat tgtggtcagt ttgttacttg ttagaatcca 180 ggtatcattt cctgatttcc t 201 <210> 36 <211> 201 <212> DNA <213> Homo sapiens <400> 36 attgcataag gatccctgta acctagtgaa cacaagcaga gaaggcaaag aacatgcaag 60 tcaatgaaat atatgtgata tgcatcactg tttgctgaca cggtagatga catttaattc 120 caatgctctt cccgccattt tgacaacttg cttatcccaa tgaactttat cactgtcatt 180 ctggatttac aagaaaattc c 201 <210> 37 <211> 201 <212> DNA <213> Homo sapiens <400> 37 ttgtgtccag tgccatcagt aaaatgggaa gtagcagaag gagatgatag gtacctagga 60 agagatttca tgagctattc aataaatttt ggatgctttt cgatatttgg aagatgtgtg 120 tccttaggag agatggcctc tacacttgaa gttactaatt catggcaaac cattctcctt 180 caaagacaaa gttttgctga a 201 <210> 38 <211> 201 <212> DNA <213> Homo sapiens <400> 38 tacaatcctt gacaacccca aatttaaatc cctctgttct gaaagcagag aatcctccca 60 agtcctcaag gacagagaat cctctgggcc ttgatactga cagtacccca gctcgctaaa 120 aatgaacctt ccaagtccct caatacaaaa ttccttgggc ctcccagccc ctgaccccag 180 atcacccctt tcctgccccc g 201 <210> 39 <211> 201 <212> DNA <213> Homo sapiens <400> 39 tgtcagctct cacagccact cctaagcctt taagtgtggg tctccccagc cccacacctg 60 ccctcccaga gctgcccaca catgtctgct aagaggtgct cgtcttcagc cgggacccaa 120 cccttgccca agggatgctc ctgctggggg ccacgaaagg aggccagctc agcagcctgc 180 ttctgccttt gctcactcca g 201 <210> 40 <211> 201 <212> DNA <213> Homo sapiens <400> 40 ttggcctccc aaagtgctgg tattataggc atgagccacc acacccaacc caatactcta 60 cattcactat tagatcctct tagtagaaac cagtatccta aaacagatgc agagtgagat 120 ttacctaaca atttagtaaa aaatatagag ctgatgtatt atggagctga tttctttatc 180 tacactggta aaaatagtga a 201 <210> 41 <211> 201 <212> DNA <213> Homo sapiens <400> 41 tctaattatc tttacagaag ccaccaactt tcttttcctt atcccctgcc ccaaggaggt 60 cacacttgga tccacccaat agtatccagt tgactacgta ggttccatag accctggtgg 120 ctgctccccc ttccttgtcc ccgccgtctc tgcccatcat cctttcacca acttaaccaa 180 atgctattgt gccttctagg g 201 <210> 42 <211> 201 <212> DNA <213> Homo sapiens <400> 42 gcccctggga agtgcttctg attttatgtc ccctgcctac agaggatgaa attgggcccg 60 agggcactga agcaagctgg gtgaagttat cttgtttctc actcttatac ttctgcgagg 120 gaggggctct tttcagtcga actccaaaaa gcttttcaac atcatcctga ttggataggt 180 ttgcatgctg gttattatta c 201 <210> 43 <211> 201 <212> DNA <213> Homo sapiens <400> 43 ctcccaccct agatggcggg ctccttgaag gttggcagtg tgtctttgtc agcttctgcc 60 tggtagtggc tggcatttga taacttgtga atgaggaaat caatgcatga ggtgacacct 120 gggggttctt ttacaagtta cagctcccgc tttaaccctg ggggtttgtt tgagcctcac 180 agtagctctg aggtagggaa g 201 <210> 44 <211> 201 <212> DNA <213> Homo sapiens <400> 44 ctcaggctgg gccagtttcc attcccccac cttatctcag actgaggtga ggtgggacat 60 gacctggtgg gacatcattt ctgaccagta gcccttccct agaatatttc tgatgctcct 120 gccaactccc tccccaattt ggtttaggat agggatgccc tctcccagga tgcagcacaa 180 ctacaatggg ttggagggcc t 201 <210> 45 <211> 201 <212> DNA <213> Homo sapiens <400> 45 gactcttggc tctgctgtag ccatcacaga taatctcttg cactggggtg ggagcatctg 60 attggctgta tttaggtcat gtgaatgtgt ctgtaactgt cagggagctg aaagaactac 120 ctgtctccct ttggcttctg tactgcttcc cacttatctt gagagtgccc ctaaacaggc 180 aaggtgttca ggtactagtc a 201 <210> 46 <211> 201 <212> DNA <213> Homo sapiens <400> 46 agctccaact tacaatttta atgaactgga ggtttgcttt ttatatgcag tcttttctcg 60 gtcttgttta aggtctctag ctttagtata ttattctcct cgtattcaaa acattcaaaa 120 gtattgaaaa ttaccaagaa aagcctcaca tgtattaatc attttctttc caaatatatt 180 agaataacag gaagaagacc a 201 <210> 47 <211> 201 <212> DNA <213> Homo sapiens <400> 47 taattaataa tatctgaatg agactctgct aggagtctta aaaaaatgag acatgctttg 60 attaaagtaa aagaatatag aatgcttctg tctataagtg aaatgacttt tgttccagaa 120 taagaaagag gatggtgaag ggcaacacaa catatagaga tctgtttgag gttcacagaa 180 agtgaatctg aaagggacca c 201 <210> 48 <211> 201 <212> DNA <213> Homo sapiens <400> 48 tttggcctct caaattgtta ggattacagg catgagccac cagctcttta ttttaccata 60 aaagttgctt ataatctgcc acctgccaat ttcttcaccc ctgttgctat tcactaagac 120 ctagtcatat gcaacttttt atttttattt tttgtttcaa actcatttta ggttcaggga 180 gtacatgtgc ggtttttcta t 201 <210> 49 <211> 201 <212> DNA <213> Homo sapiens <400> 49 atgcaatagt catcaagtgg gtaaacttaa ggtttatttg ttttattgta tataaattat 60 atctcaattg aaatgtgaaa acaaagcaat aaaacatccc ctgttcccat ttcaggagtc 120 atgtctgggc tgcctgggag gacaacagga atactgatcg ctagagcaaa taaaagactc 180 ttggcagtgg gcaacttggt c 201 <210> 50 <211> 201 <212> DNA <213> Homo sapiens <400> 50 acttcaagca gggcagagac acaactaagg ggaagcaatt ctacagttct tcctctgatg 60 actttaactg tcctttcccc acctctgagc aggttttaga atttcatatc agatgcccag 120 gcagatagga ctgatgactg tcctctggat ctcttgcccc tccctactct aggattcagt 180 ccagcatctt cactacatgc a 201 <210> 51 <211> 201 <212> DNA <213> Homo sapiens <400> 51 ttatgccctg agccccatgc tgtgggttct tgctaggaga catactctgg ggaccactcc 60 tgggccaata gttagaagtg gttgggaagc aggtcaaata gttaataggc cagacaatgg 120 cccagattgc tagtgactcc cagacagcca aagatagaaa aattttcaaa taaaaaaatt 180 acataagtag ttgggatttt g 201 <210> 52 <211> 201 <212> DNA <213> Homo sapiens <400> 52 tacttctgct cttaacgtcc cgtgactttt tggattccat tctccctcat cctaagactt 60 cagtaatcca gtcatcccct cattctcgtg tcaatttctg cccctttact ggaacagtcc 120 caaaaaccta tattctagta tctcccattt attaaaaata cctcccttaa ccccacatcc 180 tcctgctttt ctctgtcctt c 201 <210> 53 <211> 201 <212> DNA <213> Homo sapiens <400> 53 ttattaaaat gtaactttcc agagatattg acacaagtaa atatacttgt tttgaattga 60 cattcca tcctacttgc gatacatcat agtgcccttt ttctagctaa gtggtcagta ctgttagcac 180 acttaaattt tctctgtgta g 201 <210> 54 <211> 201 <212> DNA <213> Homo sapiens <400> 54 aaactctaga ctctggagag ccttgatgcc cttcctcttt taccccgtcc ctgatgctcc 60 cccaagtagc atgatgccca gtggcccacc aacctcatta cccagcattg ctgacaacag 120 gcacagcctt ggtcactgtg aaaatgcccc acagcctcaa cctcagaagt gggaagtata 180 ctgcagattc actctatgcc a 201

Claims (9)

(SNP) exists at the 101st position of the polynucleotide in 54 polynucleotides or complementary polynucleotides thereof each consisting of the nucleotide sequence of SEQ ID NO: 1 to SEQ ID NO: 54, and the 10 &lt; th &gt; A polynucleotide comprising a polynucleotide consisting of more than two consecutive nucleotides. The marker composition according to claim 1, wherein the marker composition is for confirming a kinship between a father and a daughter. A microarray for identifying a female relationship comprising the marker composition of claim 1 or claim 2. Providing an isolated nucleic acid sample; And
Determining the nucleotide at the SNP position of the polynucleotide included in the marker composition of claim 1 or 2. 5. The method of claim 4, wherein the method is for identifying a kinship between a father and a daughter. 5. The method of claim 4, wherein determining the nucleotide at the SNP position of the polynucleotide marker is performed by sequencing, hybridization with a probe, or single base primer extension. 5. The method of claim 4, wherein the method further comprises comparing the nucleotide at the SNP position of the polynucleotide included in the determined female relationship confirmation marker composition to the result of a control sample. 8. The method of claim 7, wherein the control sample is a DNA of an individual to be compared or a DNA profile of a registered individual. 5. The method according to claim 4, wherein the method further comprises identifying nucleotides at SNP positions of the polynucleotides included in the determined marker sequence composition and calculating a female-female relationship determination index.